1,059 results match your criteria mg-63 osteoblasts

Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells.

Polymers (Basel) 2021 Jul 14;13(14). Epub 2021 Jul 14.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. Read More

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Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks.

J Contemp Dent Pract 2021 Feb 1;22(2):107-110. Epub 2021 Feb 1.

Department of Periodontology, Bapuji Dental College and Hospital, Davangere, Karnataka, India.

Aim: Biomimetic implant surface coatings can improve osteoblast response and enhance osseointegration. This study aimed to assess the response of osteoblast-like cell line (MG-63) coating of injectable platelet-rich fibrin on titanium discs.

Materials And Methods: Injectable-PRF (i-PRF) was prepared by centrifugation of blood at 700 rpm for 3 minutes without any anti-coagulant in i-PRF tubes. Read More

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February 2021

Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence.

Microbiologyopen 2021 03;10(2):e1178

Department of Biomedical and Biotechnological Sciences (BIOMETEC), Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMARLab, University of Catania, Catania, Italy.

The ability of S. aureus to infect bone and osteoblasts is correlated with its incredible virulence armamentarium that can mediate the invasion/internalization process, cytotoxicity, membrane damage, and intracellular persistence. We comparatively analyzed the interaction, persistence, and modulation of expression of selected genes and cell viability in an ex vivo model using human MG-63 osteoblasts of two previously studied and well-characterized S. Read More

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The impact of E-cigarette vaping and vapour constituents on bone health.

J Inflamm (Lond) 2021 May 5;18(1):16. Epub 2021 May 5.

Institute of Inflammation and Ageing, MRC-ARUK Centre for Musculoskeletal Ageing Research, University of Birmingham, Birmingham, B15 2TT, UK.

Background: In contrast to cigarettes, electronic cigarette use (E-cigarettes) has grown substantially over the last decade. This is due to their promotion as both a safer alternative to cigarettes and as an aide to stop smoking. Critically, upon E-cigarette use, the user may be exposed to high doses of nicotine in addition to other compounds including flavouring chemicals, metal particulates and carbonyl compounds, particularly in highly vascularised tissues such as bone. Read More

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An organic-inorganic hybrid scaffold with honeycomb-like structures enabled by one-step self-assembly-driven electrospinning.

Mater Sci Eng C Mater Biol Appl 2021 May 27;124:112079. Epub 2021 Mar 27.

Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland; Helsinki Institute of Life Science (HiLIFE), University of Helsinki, FI-00014 Helsinki, Finland. Electronic address:

Electrospun organic/inorganic hybrid scaffolds have been appealing in tissue regeneration owing to the integrated physicochemical and biological performances. However, the conventional electrospun scaffolds with non-woven structures usually failed to enable deep cell infiltration due to the densely stacked layers among the fibers. Herein, through self-assembly-driven electrospinning, a polyhydroxybutyrate/poly(ε-caprolactone)/58S sol-gel bioactive glass (PHB/PCL/58S) hybrid scaffold with honeycomb-like structures was prepared by manipulating the solution composition and concentration during a one-step electrospinning process. Read More

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Synthesis and characterization of nano-hydroxyapatite from Sardinella longiceps fish bone and its effects on human osteoblast bone cells.

J Mech Behav Biomed Mater 2021 07 14;119:104501. Epub 2021 Apr 14.

CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, India.

Organic debris in the form of fish bone wastes account to several thousand tons annually. In recent years, researchers have turned attention towards the bioconversion of organic debris into materials with biomedical applications. Accordingly, the present study synthesized nano-Hydroxyapatite (n-HAP) from bones of discarded Sardinella longiceps by the alkaline hydrolysis method. Read More

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Investigation of Mg-xLi-Zn alloys for potential application of biodegradable bone implant materials.

J Mater Sci Mater Med 2021 Apr 6;32(4):43. Epub 2021 Apr 6.

School of Materials Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mold Technology (Ministry of Education), Zhengzhou University, Zhengzhou, 450001, China.

Implant therapy after osteosarcoma surgery is a major clinical challenge currently, especially the requirements for mechanical properties, degradability of the implants, and their inhibition of residual tumor cells. Biodegradable magnesium (Mg) alloy as medical bone implant material has full advantages and huge potential development space. Wherein, Mg-lithium (Li) based alloy, as an ultra-light alloy, has good properties for implants under certain conditions, and both Mg and Li have inhibitory effects on tumor cells. Read More

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The Impacts of Crystalline Structure and Different Surface Functional Groups on Drug Release and the Osseointegration Process of Nanostructured TiO.

Molecules 2021 Mar 19;26(6). Epub 2021 Mar 19.

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30387 Krakow, Poland.

In implantable materials, surface topography and chemistry are the most important in the effective osseointegration and interaction with drug molecules. Therefore, structural and surface modifications of nanostructured titanium dioxide (TiO) layers are reported in the present work. In particular, the modification of annealed TiO samples with -OH groups and silane derivatives, confirmed by X-ray photoelectron spectroscopy, is shown. Read More

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Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Kraków, Poland.

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Read More

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Effects of Er:YAG laser irradiation of different titanium surfaces on osteoblast response.

J Mater Sci Mater Med 2021 Mar 6;32(3):22. Epub 2021 Mar 6.

Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.

The aim of this in vitro study was to evaluate the effects of erbium-doped yttrium aluminum garnet (Er:YAG) laser irradiation on titanium surface topography and the proliferation and differentiation of osteoblasts using standard clinical treatment settings. Er:YAG laser irradiation at two levels ((1): 160 mJ, pulse at 20 Hz; (2): 80 mJ, pulse at 20 Hz) was applied to moderately rough and smooth titanium disks before MG-63 osteoblast-like cells were cultured on these surfaces. Titanium surface and cell morphology were observed by scanning electron microscopy. Read More

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Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?

Pathogens 2021 Feb 19;10(2). Epub 2021 Feb 19.

Medical Molecular Microbiology and Antibiotic Resistance Laboratory (MMARLab), Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95125 Catania, Italy.

Bacterial internalization is a strategy that non-intracellular microorganisms use to escape the host immune system and survive inside the human body. Among bacterial species, showed the ability to interact with and infect osteoblasts, causing osteomyelitis as well as bone and joint infection, while also becoming increasingly resistant to antibiotic therapy and a reservoir of bacteria that can make the infection difficult to cure. Despite being a serious issue in orthopedic surgery, little is known about the mechanisms that allow bacteria to enter and survive inside the osteoblasts, due to the lack of consistent experimental models. Read More

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February 2021

Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties.

Materials (Basel) 2021 Feb 8;14(4). Epub 2021 Feb 8.

Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.

The surface modification of titanium substrates and its alloys in order to improve their osseointegration properties is one of widely studied issues related to the design and production of modern orthopedic and dental implants. In this paper, we discuss the results concerning Ti6Al4V substrate surface modification by (a) alkaline treatment with a 7 M NaOH solution, and (b) production of a porous coating (anodic oxidation with the use of potential U = 5 V) and then treating its surface in the abovementioned alkaline solution. We compared the apatite-forming ability of unmodified and surface-modified titanium alloy in simulated body fluid (SBF) for 1-4 weeks. Read More

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February 2021

Suturable elastomeric tubular grafts with patterned porosity for rapid vascularization of 3D constructs.

Biofabrication 2021 Jan 22. Epub 2021 Jan 22.

ICPEES, University of Strasbourg, 25 rue Bécquerel, Strasbourg, 67087, FRANCE.

Vascularization is considered to be one of the key challenges in engineering functional 3D tissues. Engineering suturable vascular grafts containing pores with diameter of several tens of microns in tissue engineered constructs may provide an instantaneous blood perfusion through the grafts improving cell infiltration and thus, allowing rapid vascularization and vascular branching. The aim of this work was to develop suturable tubular scaffolds to be integrated in biofabricated constructs, enabling the direct connection of the biofabricated construct with the host blood stream, providing an immediate blood flow inside the construct. Read More

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January 2021

Study of the Synergistic Effect of an Enzyme Cocktail and Antibiotics against Biofilms in a Prosthetic Joint Infection Model.

Antimicrob Agents Chemother 2021 03 18;65(4). Epub 2021 Mar 18.

Université catholique de Louvain, Louvain Drug Research Institute, Pharmacologie cellulaire et moléculaire, Brussels, Belgium

Prosthetic joint infections (PJI) are frequent complications of arthroplasties. Their treatment is made complex by the rapid formation of bacterial biofilms, limiting the effectiveness of antibiotic therapy. In this study, we explore the effect of a tri-enzymatic cocktail (TEC) consisting of an endo-1,4-β-d-glucanase, a β-1,6-hexosaminidase, and an RNA/DNA nonspecific endonuclease combined with antibiotics of different classes against biofilms of , , and grown on Ti-6Al-4V substrates. Read More

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Different Modulatory Effects of Four Methicillin-Resistant Clones on MG-63 Osteoblast-Like Cells.

Biomolecules 2021 01 7;11(1). Epub 2021 Jan 7.

Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95125 Catania, Italy.

is a Gram-positive bacterium responsible for a variety of mild to life-threatening infections including bone infections such as osteomyelitis. This bacterium is able to invade and persist within non-professional phagocytic cells such as osteoblasts. In the present study, four different strains, namely, 2SA-ST239-III (ST239), 5SA-ST5-II (ST5), 10SA-ST228-I (ST228), and 14SA-ST22-IVh (ST22), were tested for their ability to modulate cell viability in MG-63 osteoblast-like cells following successful invasion and persistence. Read More

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January 2021

MACC1 Contributes to the Development of Osteosarcoma Through Regulation of the HGF/c-Met Pathway and Microtubule Stability.

Front Cell Dev Biol 2020 23;8:825. Epub 2020 Dec 23.

Department of Orthopedics, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.

Osteosarcoma (OS) is the most prevalent human bone malignancy, and presents a global annual morbidity of approximately five cases per million. Notably, precise and efficient targeted therapy has become the most promising strategy for the treatment of OS; however, there is still an urgent need for the identification of suitable therapeutic targets. Metastasis-associated in colon cancer 1 (MACC1) was first identified in colon tumors by differential display RT-PCR, and was shown to be involved in the regulation of colon tumor growth and metastasis through the hepatocyte growth factor (HGF)/c-Met signaling pathway. Read More

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December 2020

Osteogenic differentiation and mineralization potential of zinc oxide nanoparticles from Scutellaria baicalensis on human osteoblast-like MG-63 cells.

Mater Sci Eng C Mater Biol Appl 2021 Feb 21;119:111656. Epub 2020 Oct 21.

Department of Biochemistry, Panimalar Medical College Hospital & Research Institute, (Affiliated to The Tamil Nadu Dr. MGR Medical University), Varadharajapuram, Poonamallee, Chennai 600 123, India. Electronic address:

Development of biologically inspired green synthesis of silver nanoparticles has been extensively scrutinized owing to its uses in biomedical industry. In the last two decades, the demands of nanomaterial in bone remodelling have increased. Scutellaria baicalensis is a flowering plant usually used for many ailments. Read More

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February 2021

Toxic mechanisms of Roth801, Canals, microparticles and nanoparticles of ZnO on MG-63 osteoblasts.

Mater Sci Eng C Mater Biol Appl 2021 Feb 17;119:111635. Epub 2020 Oct 17.

School of Dentistry, National Taiwan University Medical College, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Electronic address:

ZnO eugenol-based materials are widely used for restoration of caries cavity, apical retrograde filling and root canal sealer. Their effects on apical bone healing await investigation. The toxic mechanisms of ZnO particles and nanoparticles to MG-63 osteoblastic cells were studied. Read More

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February 2021

Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide.

Nanomaterials (Basel) 2020 Dec 7;10(12). Epub 2020 Dec 7.

School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.

Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α-TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. Read More

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December 2020

The interactions between mTOR and NF-κB: A novel mechanism mediating mechanical stretch-stimulated osteoblast differentiation.

J Cell Physiol 2020 Dec 2. Epub 2020 Dec 2.

Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.

Mechanical stretch is known to promote osteoblast differentiation in vitro and accelerate bone regeneration in vivo, whereas the relevant mechanism remains unclear. Recent studies have shown the importance of reciprocal interactions between mammalian target of rapamycin (mTOR) and nuclear factor kappa B (NF-κB; two downstream molecules of Akt) in the regulation of tumor cells. Thus, we hypothesize that mTOR and NF-κB as well as their interconnection play a critical role in mediating stretch-induced osteogenic differentiation in osteoblasts. Read More

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December 2020

Bioactivity in SBF versus trace element effects: The isolated role of Mg and Zn in osteoblast behavior.

Mater Sci Eng C Mater Biol Appl 2021 Jan 4;118:111320. Epub 2020 Aug 4.

Department of Materials Science and Engineering, Federal University of Sergipe, Av. Marechal Rondon, s/n, São Cristóvão, 49100-000 Sergipe, Brazil. Electronic address:

The bioactivity assay originally proposed by Kokubo is one of the most commonly used tests to indirectly evaluate the biocompatibility of bioactive glasses. However, extensive evidence has shown that trace elements present in biomaterials may stimulate cellular behavior in different ways even when no apatite formation is observed, i.e. Read More

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January 2021

The Anchorage of Bone Cells onto an Yttria-Stabilized Zirconia Surface with Mild Nano-Micro Curved Profiles.

Dent J (Basel) 2020 Nov 10;8(4). Epub 2020 Nov 10.

Department of Cell Biology, University Medical Center Rostock, D-18057 Rostock, Germany.

The high biocompatibility, good mechanical properties, and perfect esthetics of ceramic dental materials motivate investigation into their suitability as an endosseous implant. Osseointegration at the interface between bone and implant surface, which is a criterion for dental implant success, is dependent on surface chemistry and topography. We found out earlier that osteoblasts on sharp-edged micro-topographies revealed an impaired cell phenotype and function and the cells attempted to phagocytize these spiky elevations in vitro. Read More

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November 2020

Expression profiles of the Wnt/β-catenin signaling-related extracellular antagonists during proliferation and differentiation in human osteoblast-like cells.

Exp Ther Med 2020 Dec 23;20(6):254. Epub 2020 Oct 23.

Genomics of Bone Metabolism Laboratory, National Institute of Genomic Medicine (INMEGEN), Mexico City 14610, Mexico.

Bone formation is a dynamic process directed by osteoblast activity. The transition from the proliferation to differentiation stage during osteoblast maturation involves the downregulation of the Wnt/β-catenin signaling pathway, and extracellular antagonists are important for the regulation of Wnt signaling. However, the expression levels of Wnt antagonists in these stages of human osteoblast maturation have not been fully elucidated. Read More

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December 2020

Porous silicon carbide coated with tantalum as potential material for bone implants.

Regen Biomater 2020 Oct 18;7(5):453-459. Epub 2020 Jun 18.

Orthopaedic Department Affiliated ZhongShan Hospital of Dalian University, No. 6 Jiefang Street, Zhongshan District, Dalian, Liaoning 116001, China.

Porous silicon carbide (SiC) has a specific biomorphous microstructure similar to the trabecular microstructure of human bone. Compared with that of bioactive ceramics, such as calcium phosphate, SiC does not induce spontaneous interface bonding to living bone. In this study, bioactive tantalum (Ta) metal deposited on porous SiC scaffolds by chemical vapour deposition was investigated to accelerate osseointegration and improve the bonding to bones. Read More

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October 2020

Lanthanides-Substituted Hydroxyapatite/ Composite Coated Titanium Plate for Bone Tissue Regeneration.

Int J Nanomedicine 2020 27;15:8261-8279. Epub 2020 Oct 27.

Orthopaedic Department of Xijing Hospital of the Fourth Military Medical University, Xi'an, Shaanxi, 710032, Mainland China.

Purpose: To develop the surface-treated metal implant with highly encouraged positive properties, including high anti-corrosiveness, bio-activeness and bio-compatibleness for orthopedic applications.

Methods: In this work, the surface of commercially pure titanium (Ti) metal was treated with bio-compatible polydopamine (PD) by merely immersing the Ti plate in PD solution. The composite of trivalent lanthanide minerals (La, Ce and Gd)-substituted hydroxyapatite (MHAP) with (AV) gel was prepared and coated on the PD-Ti plate by electrophoretic deposition (EPD) method. Read More

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December 2020

Administration of mircoRNA-135b-reinforced exosomes derived from MSCs ameliorates glucocorticoid-induced osteonecrosis of femoral head (ONFH) in rats.

J Cell Mol Med 2020 12 22;24(23):13973-13983. Epub 2020 Oct 22.

Department of Internal Medicine of TCM, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Zhejiang University of Traditional Chinese Medicine, Wenzhou, China.

Exosomes were found to exert a therapeutic effect in the treatment of osteonecrosis of the femoral head (ONFH), while miR-135b was shown to play an important role in the development of ONFH. In this study, we investigated the effects of concomitant administration of exosomes and miR-135b on the treatment of ONFH. A rat mode of ONFH was established. Read More

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December 2020

MicroRNA-150-5p inhibits proliferation and invasion of osteosarcoma cells by down-regulating VEGFA.

Y Qin B Zhang B-J Ge

Eur Rev Med Pharmacol Sci 2020 09;24(18):9265-9273

Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Objective: The aim of this study was to investigate whether microRNA-150-5p was involved in osteosarcoma cell proliferation and invasiveness via modulating vascular endothelial growth factor A (VEGFA) expression.

Patients And Methods: 10 pairs of osteosarcoma tissues and para-cancerous tissues were collected from patients with osteosarcoma in our center from February 2012 to July 2018. Relative expression levels of microRNA-150-5p and VEGFA in tissues and cell lines were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Read More

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September 2020

Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells.

Sci Rep 2020 09 16;10(1):15219. Epub 2020 Sep 16.

Division of Life Sciences, College of Life Science and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon, 22012, Republic of Korea.

In this work, the influence of parylene N film on the spheroid formation of osteoblast-like cells (MG-63) was determined and compared with that of high-hydrophilicity microenvironments, such as hydrophilic culture matrix and ultraviolet-treated parylene N film. To elucidate the change in cell properties due to the microenvironment of parylene N film, global gene expression profiles of MG-63 cells on parylene N film were analyzed. We confirmed the upregulated expression of osteoblast differentiation- and proliferation-related genes, such as Runx2, ALPL, and BGLAP and MKi67 and PCNA, respectively, using the real-time polymerase chain reaction. Read More

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September 2020

Agar/κ-carrageenan/montmorillonite nanocomposite hydrogels for wound dressing applications.

Int J Biol Macromol 2020 Dec 12;164:4591-4602. Epub 2020 Sep 12.

Akdeniz University, Faculty of Science, Department of Chemistry, Antalya, Turkey. Electronic address:

In this study, agar/κ-carrageenan/montmorillonite (MMT) hydrogels were prepared to examine their usability as wound dressing materials and to see the effect of MMT amount on some properties of agar/κ-carrageenan hydrogel materials. Hydrogels were characterized by SEM-EDX, TEM and DSC analyses. By increasing the MMT content within hydrogel matrix from 0% to 5%, the decomposition temperature of the hydrogel material was increased from 256. Read More

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December 2020

Efficacy of Plasma-Polymerized Allylamine Coating of Zirconia after Five Years.

J Clin Med 2020 Aug 27;9(9). Epub 2020 Aug 27.

Biomaterials and Technology, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, 4058 Basel, Switzerland.

Plasma-polymerized allylamine (PPAAm) coatings of titanium enhance the cell behavior of osteoblasts. The purpose of the present study was to evaluate a PPAAm nanolayer on zirconia after a storage period of 5 years. Zirconia specimens were directly coated with PPAAm (ZA0) or stored in aseptic packages at room temperature for 5 years (ZA5). Read More

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