35,163 results match your criteria methods gc-ms

Antimicrobial and anticancer properties of Carica papaya leaves derived di-methyl flubendazole mediated silver nanoparticles.

J Infect Public Health 2021 Feb 20;14(5):577-587. Epub 2021 Feb 20.

Department of Biotechnology, Prathyusha Engineering College, Chennai 602025, India. Electronic address:

Background: In this study, a biologically active molecule, di-methyl flubendazole isolated from the extract of Carica papaya leaves confirmed by using GC-MS, H NMR, and C NMR analysis was applied to synthesize silver nanoparticles (AgNPs). The AgNPs with plant sources an alternative therapeutic agent for synthetic compound used in cancer chemotherapy.

Methods: The AgNPs were characterized using UV, FT-IR, XRD, FESEM with EDX and TEM. Read More

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February 2021

Deep eutectic solvent-homogenate based microwave-assisted hydrodistillation of essential oil from Litsea cubeba (Lour.) Pers. fruits and its chemical composition and biological activity.

J Chromatogr A 2021 Mar 22;1646:462089. Epub 2021 Mar 22.

School of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China. Electronic address:

As an important natural product, the sufficient separation of plant essential oil (EO) is helpful to improve its utilization value. In this work, deep eutectic solvent-homogenate based microwave-assisted hydrodistillation (DES-HMAHD) was developed and applied to isolate EO from the fruits of Litsea cubeba (Lour.) Pers. Read More

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Antidiabetic effect of seeds extract in streptozotocin-induced diabetic mice: a statistical evaluation.

J Investig Med 2021 Apr 12. Epub 2021 Apr 12.

Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran.

Undoubtedly, identification of the chemical composition of organic extracts or secondary metabolites of plant materials and evaluation of their potential bioactivity are among the main objectives of natural products-based investigations. In the present study, we report the chemical composition and antidiabetic activity of (Family Fabaceae) seeds extract (SPE) for the first time. First, the plant seeds were macerated in ethanol. Read More

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Investigation of the idiosyncratic hepatotoxicity of Polygonum multiflorum Thunb. through metabolomics using GC-MS.

BMC Complement Med Ther 2021 Apr 12;21(1):120. Epub 2021 Apr 12.

College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.

Background: The idiosyncratic hepatotoxicity of Polygonum multiflorum (PM) has attracted considerable interest, but the idiosyncratically hepatotoxic components and endogenous metabolite changes resulting from idiosyncratic hepatotoxicity of PM are not well understood. The aim of this study was to identify the idiosyncratically hepatotoxic components and potential endogenous metabolic biomarkers for PM-induced liver injury.

Methods: Serum biochemical indicators and hematoxylin and eosin (H&E) staining were evaluated to identify pathological changes. Read More

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Effects of Sambucus Ebulus Extract on Cell Proliferation and Viability of Triple-Negative Breast Cancer: An In Vitro and In Vivo Study.

Anticancer Agents Med Chem 2021 Apr 11. Epub 2021 Apr 11.

Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran. Iran.

Background: Triple-negative breast cancer (TNBC) accounts for 15% of all breast cancer (BC) cases and is a severe type of BC. Since medicinal herbs containing biocompatible substances that are accepted by patient more than chemical therapeutics, they can be considered a safe option for treating BC.

Objective: This study evaluated the effect of Sambucus Ebulus (S. Read More

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Integrated metabolomics and network pharmacology approach to exploring the potential mechanism of Tianxiang Capsule for treating motion sickness.

J Ethnopharmacol 2021 Apr 9:114107. Epub 2021 Apr 9.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address:

Ethnopharmacological Relevance: Motion sickness is a multi-system syndrome caused by abnormal spatial environmental sensory conflicts. Tianxiang Capsule (TXC) is a traditional Chinese medicine (TCM) formula for the prevention and treatment of motion sickness for years. However, the main active components of TXC and mechanism of its therapeutic effects on motion sickness are still unclear. Read More

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Mapping metabolome changes in Mill fruits at different maturation stages MS-based metabolomics and chemometrics.

J Adv Res 2021 Mar 24;29:179-189. Epub 2019 Oct 24.

Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini St., P.B. 11562, Cairo, Egypt.

Introduction: Mill, sponge gourd or Egyptian cucumber, is grown worldwide for its edible fruit consumed as a vegetable like cucumber. Unlike young fruit (YF), the fully mature ripened fruit (MF) is strongly fibrous and is used as a cleanser to make scrubbing bath sponges. YF undergoes a complex series of physiological and biochemical changes during fruit ripening. Read More

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Identification and quantification of phenolic and fatty acid profiles in mill. seeds by LC-ESI-MS and GC: Effect of drying methods on chemical composition.

Food Sci Nutr 2021 Apr 25;9(4):1907-1916. Epub 2021 Feb 25.

Laboratory of Organic Chemistry LR17ES08 Sciences Faculty of Sfax University of Sfax Sfax Tunisia.

This study aimed to evaluate Mill. seeds oil as well as methanolic-aqueous extract on fatty acid and phenolic's composition as well as antioxidant activities with regard to the cones drying methods: convection and sun methods. The highest amounts of total phenols (14. Read More

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Leishmanicidal, Trypanocidal and Antioxidant Activity of Amyrin-Rich Extracts from Cambess.

Iran J Pharm Res 2020 ;19(4):343-353

Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel/PR, Brazil.

Aims: This study aimed to characterize and evaluate leishmanicidal and trypanocidal action as well as cytotoxicity on macrophages and antioxidant ability of extracts, obtained by supercritical CO and ultrasound-assisted extractions of Uvaia () leaves.

Methods: Leaves from were submitted to supercritical CO (E1) and ultrasound-assisted (E2) extractions. The characterization of extracts was done using GC-MS and HPLC. Read More

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January 2020

Clinical, Biochemical, and Molecular Analyses of Medium-Chain Acyl-CoA Dehydrogenase Deficiency in Chinese Patients.

Front Genet 2021 23;12:577046. Epub 2021 Mar 23.

Department of Pediatric Endocrinology/Genetics, Shanghai Institute for Pediatric Research, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Objective: Medium-chain acyl-CoA dehydrogenase deficiency (MCADD) is a rare inherited metabolic disorder of fatty acid β-oxidation. The present study aimed to evaluate clinical and biochemical manifestations, and the mutation spectrum of this disorder in a large cohort of Chinese patients.

Methods: A total of 24 patients were enrolled, and blood acylcarnitine and urinary organic acid levels were measured by tandem mass spectrometry and gas chromatography-mass spectrometry (GC-MS), respectively. Read More

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Excessive Intake of Longan Arillus Alters gut Homeostasis and Aggravates Colitis in Mice.

Front Pharmacol 2021 26;12:640417. Epub 2021 Mar 26.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.

Longan is the fruit of Lour. and the longan arillus has long been used in traditional Chinese medicine possessing various health benefits. However, the excessive intake of longan is found in daily life to cause "" syndrome. Read More

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Off-flavor in soy drink: Development, optimization, and validation of an easy and fast method to quantify the key odorants.

Talanta 2021 Jul 13;229:122251. Epub 2021 Mar 13.

Institute of Food Science and Biotechnology, Department of Flavor Chemistry, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany. Electronic address:

A detailed molecular flavor profile was necessary to understand the low acceptance of soy drink by Western consumers. Accordingly, key odor-active compounds were detected by means of aroma dilution analyses coupled with gas chromatography-mass spectrometry-olfactometry after application of suitable solvent-free volatile extraction techniques. Four quantification methods (standard addition, external calibration, internal standard, and stable isotope dilution assay) were developed and validated to measure the concentrations after direct immersion-stir bar sorptive extraction. Read More

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Metabolomic analysis of Schoenoplectus juncoides reveals common markers of acetolactate synthase inhibition among paddy weeds.

Pestic Biochem Physiol 2021 May 10;174:104827. Epub 2021 Mar 10.

Health & Crop Science Research Laboratory, Sumitomo Chemical Co., Ltd, Takarazuka, Hyogo 665-8555, Japan. Electronic address:

Despite the increase in pressure for reducing the use of chemical pesticides in agriculture, herbicides remain one of the efficient tools for augmenting food production. Various herbicide-resistant weeds against most herbicidal modes of action (MoA) are emerging worldwide, and therefore, the necessity of developing herbicides with novel MoA is increasing. Toward this, rigid methods of determining MOA that can be applied for various weeds species are required. Read More

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Hollow Electrode Capillary Plasma Ionization Source for Rapid On-line Detection of Gaseous Samples.

Rapid Commun Mass Spectrom 2021 Apr 9:e9099. Epub 2021 Apr 9.

Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, Ningbo University, Ningbo, 315211, China.

Rationale: Gas Chromatography Mass Spectrometry (GC-MS) with electron ionization (EI)is the most widely used analysis technique of gaseous samples, but it may be time-consuming for on-line monitoring of mixtures whose concentrations relatively change rapidly. On the other hand, current ionization methods, such as chemical ionization (CI) and proton transfer reaction (PTR), also have some disadvantages such as selectivity. Appropriate soft ionization sources therefore are searched for rapid on-line detection. Read More

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Characterization of Naturally Occurring Alpha-Diketone Emissions and Exposures at a Coffee Roasting Facility and Associated Retail Café.

Ann Work Expo Health 2021 Apr 10. Epub 2021 Apr 10.

Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, USA.

Background: Alpha-diketones such as diacetyl and 2,3-pentanedione have been used as artificial flavorings in a variety of industries and are produced naturally when food products such as coffee beans are roasted. Exposure to these compounds has been associated with bronchiolitis obliterans, a rare and severe respiratory disease. In the current paper, we (i) evaluate which steps in the coffee production process are associated with the highest alpha-diketone emissions at a small craft coffee roaster and associated café, (ii) determine the extent to which direct-reading measurements of CO, CO2, and total volatile organic compounds (VOCs) can serve as lower-cost surrogate indicators for diacetyl concentrations, and (iii) conduct a limited emissions study to quantify the effect that the process variable of roast type has on diacetyl emissions from grinding beans. Read More

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Improving the patient-centred care of children with life-altering skin conditions using feedback from electronic patient-reported outcome measures: protocol for a hybrid effectiveness-implementation study (PEDS-ePROM).

BMJ Open 2021 Apr 9;11(4):e041861. Epub 2021 Apr 9.

Centre for Children's Burns and Trauma Research, Child Health Research Centre, The University of Queensland, Saint Lucia, Queensland, Australia.

Introduction: Using patient-reported outcome measures (PROMs) with children have been described as 'giving a voice to the child'. Few studies have examined the routine use of these measures as potentially therapeutic interventions. This study aims to investigate: (1) the of feedback using graphical displays of information from electronic PROMs (ePROMs) that target health-related quality of life, to improve health outcomes, referrals and treatment satisfaction and (2) the of ePROMs and graphical displays by assessing acceptability, sustainability, cost, fidelity and context of the intervention and study processes. Read More

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Downregulation of inflammatory mediators by ethanolic extract of Bergenia ligulata (Wall.) in oxalate injured renal epithelial cells.

J Ethnopharmacol 2021 Apr 6:114104. Epub 2021 Apr 6.

Amity Institute of Biotechnology, Amity University, Noida, India. Electronic address:

Ethnopharmacological relevance-In the Indian traditional system of medicine, Bergenia ligulata (Wall.) Engl has been used for treatment of urolithiasis. Its efficacious nature has led to its incorporation in various commercial herbal formulations such as Cystone and Neeri, which are prescribed for kidney related ailments. Read More

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aromatic water: A traditional drink with considerable anti- activity.

Curr Med Mycol 2020 Sep;6(3):1-8

Department of Pharmacognosy, Shiraz University of Medical Sciences, Shiraz, Iran.

Background And Purpose: Aromatic waters (AWs) are therapeutic distillates, which harbor both essential oil and water-soluble components of a plant. Due to the dispersion of the light amount of essence through the AWs, they have their specific pleasant smell, taste, and medicinal properties. In Iranian traditional medicine, AW is used to treat gastrointestinal disorders. Read More

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September 2020

The Potential Regulatory Network of Glutamate Metabolic Pathway Disturbance in Chinese Han Withdrawal Methamphetamine Abusers.

Front Genet 2021 23;12:653443. Epub 2021 Mar 23.

Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objects: To explore the long-term influence of methamphetamine abuse on metabolomics character, with gas chromatography-mass spectrometry (GS-MS) technology, and the potential regulatory network using the bioinformatics method.

Methods: Forty withdrawal methamphetamine abusers (WMA) were recruited from Shanghai Gaojing Forced Isolation Detoxification Institute. Forty healthy controls (HC) were recruited from society. Read More

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A novel approach for lavender essential oil authentication and quality assessment.

J Pharm Biomed Anal 2021 Mar 27;199:114050. Epub 2021 Mar 27.

National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA. Electronic address:

Currently, the quality of lavender (Lavandula angustifolia Mill.) essential oil (LEO) is defined and regulated based on standards and methods established by regulatory authorities. Unfortunately, these existing standards and methods are not sufficient for LEO quality evaluation due to the complexity of LEO and adulteration encouraged by a burgeoning market. Read More

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Andrographis paniculata (Burm.f.) Nees: Traditional uses, phytochemistry, pharmacological properties and quality control/quality assurance.

J Ethnopharmacol 2021 Apr 5:114054. Epub 2021 Apr 5.

Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India. Electronic address:

Ethnopharmacological Relevance: Andrographis paniculata (Burm.f.) Nees is a medicinal herb of the Asian countries used in many traditional medicinal systems for the treatment of diarrhea, flu, leprosy, leptospirosis, malaria, rabies, upper respiratory infections, sinusitis, syphilis, tuberculosis and HIV/AIDS etc. Read More

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Snapshot of narcotic drugs and psychoactive substances in Kuwait: analysis of illicit drugs use in Kuwait from 2015 to 2018.

BMC Public Health 2021 Apr 7;21(1):671. Epub 2021 Apr 7.

Narcotic and Psychotropic Laboratory, General Department of Criminal Evidence, Ministry of Interior, Al-Dhajeej, Block 1, Al-Farwaniya City, Kuwait.

Background: The misuse of illicit substances is associated with increased morbidity and mortality; thus, substance abuse is a global health concern. The Arabian Gulf region is considered a crossing point and a consumer of illicit drugs. However, a lack of laboratory-based research has limited the scientific assessment of drug misuse in the Arabian Gulf region. Read More

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Efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.

Aybast Er Nder

J Tradit Chin Med 2021 04;41(2):293-300

Bursa Uludag University, Faculty of Science and Arts, Department of Chemistry, Bursa 16059, Turkey.

Objective: To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.

Methods: Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2'-azinobis (3-ethylbenzothiazoline-6-suphonic acid) di-ammonium salt (ABTS), Folin-Ciocalteu and high-performance liquid chromatography (HPLC) methods. The effect against oxidative DNA base damage was determined analyzing oxidative DNA base damage products by GC-MS/MS. Read More

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Caspase Cascade Activation During Apoptotic Cell Death of Human Lung Carcinoma Cells A549 Induced by Marine Sponge .

Drug Des Devel Ther 2021 29;15:1357-1368. Epub 2021 Mar 29.

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia.

Introduction: In this study, (CA), one of the most popular marine sponges for cancer therapy research, was investigated for its phytochemical compounds and evaluated for its anticancer activity in various cell lines. Since lung cancer is the most frequently diagnosed cancer, a solution from this marine source is a good choice to address the resistance to anticancer agents. Elucidation of the underlying mechanism of cell death elicited by a CA extract in human lung carcinoma cells A549 was undertaken. Read More

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Impact of gestational and postmenstrual age on excretion of fetal zone steroids in preterm infants determined by GC-MS.

J Clin Endocrinol Metab 2021 Apr 4. Epub 2021 Apr 4.

Neonatal Neuroprotection Laboratory, Department of Neonatology and Pediatric Intensive Care, University Medicine Greifswald, Sauerbruchstraße, Greifswald, Germany.

Context: Fetal zone steroids (FZS) are excreted in high concentrations in preterm infants. Experimental data suggest protective effects of FZS in models of neonatal disease.

Objective: We aimed to characterize the postnatal FZS metabolome of well preterm and term infants. Read More

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DNA methylation of tumour necrosis factor (TNF) alpha gene is associated with specific blood fatty acid levels in a gender-specific manner.

Mol Genet Genomic Med 2021 Apr 5:e1679. Epub 2021 Apr 5.

Translational Chemical Biology (TCB) Research Group, Loughborough, University, Loughborough, UK.

Background: Fatty acids, specifically polyunsaturated fatty acids (PUFAs) play an important role in inflammation and its resolution, however, their interaction with the epigenome is relatively unexplored. Here we investigate the relationship between circulating blood fatty acids and the DNA methylation of the cytokine encoding gene tumour necrosis factor (TNF, OMIM 191160).

Methods: Using a cross-sectional study approach, we collected blood samples from adults (N=88 (30 males, 58 females); 18-74 years old) for DNA methylation pyrosequencing analysis at four sites in TNF exon 1 and gas-chromatography mass-spectrometry analysis of the fatty acid profile of dried blood spots (DBS). Read More

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Evaluation of a Nanoparticle-Based Busulfan Immunoassay for Rapid Analysis on Routine Clinical Analyzers.

Ther Drug Monit 2021 Mar 9. Epub 2021 Mar 9.

Saladax Biomedical, Inc., Bethlehem, PA Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA Department of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX.

Background: Busulfan is an alkylating agent used in allogeneic hematopoietic stem cell transplantation for various malignant and non-malignant disorders. Therapeutic drug monitoring of busulfan is common, as busulfan exposure has been linked to veno-occlusive disease, disease relapse, and failed engraftment. The authors developed an automated immunoassay, along with stable calibrators and controls, and quantified busulfan in sodium heparin plasma. Read More

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Development of a new generic extraction method for the analysis of pesticides, mycotoxins, and polycyclic aromatic hydrocarbons in representative animal feed and food samples.

Food Chem 2021 Mar 22;356:129653. Epub 2021 Mar 22.

National Food Institute, Technical University of Denmark, Kemitorvet, 2800 Kgs. Lyngby, Denmark.

Various generic extraction methods have been used to determine pesticide residues, mycotoxins, and polycyclic aromatic hydrocarbons (PAHs) in food and animal feed to ensure consumer safety. However, these methods cannot extract all relevant compounds at an acceptable rate of recovery. This study presents a new extraction method. Read More

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Changing in Larch Sapwood Extractives Due to Distinct Ionizing Radiation Sources.

Materials (Basel) 2021 Mar 26;14(7). Epub 2021 Mar 26.

Department Forest Products Technology and Timber Constructions, Salzburg University of Applied Sciences, Markt 136a, 5431 Kuchl, Austria.

Wood extractives have an influence on different material properties. This study deals with the changes in wood extractives of larch sapwood due to two different low doses of energy irradiations. Electron beam irradiation (EBI) and γ-ray irradiation treatments were done by using two industrial processes. Read More

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A Toolbox for the Determination of Nitroaromatic Explosives in Marine Water, Sediment, and Biota Samples on Femtogram Levels by GC-MS/MS.

Toxics 2021 Mar 16;9(3). Epub 2021 Mar 16.

Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig-Holstein, Brunswiker Straße 10, 24105 Kiel, Germany.

To determine the amount of the explosives 1,3-dinitrobenzene, 2,4-dinitrotoluene, 2,4,6-trinitrotoluene, and its metabolites in marine samples, a toolbox of methods was developed to enhance sample preparation and analysis of various types of marine samples, such as water, sediment, and different kinds of biota. To achieve this, established methods were adapted, improved, and combined. As a result, if explosive concentrations in sediment or mussel samples are greater than 10 ng per g, direct extraction allows for time-saving sample preparation; if concentrations are below 10 ng per g, techniques such as freeze-drying, ultrasonic, and solid-phase extraction can help to detect even picogram amounts. Read More

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