106,183 results match your criteria methods discuss

Improving laboratory diagnostics in myasthenia gravis.

Expert Rev Mol Diagn 2021 May 10. Epub 2021 May 10.

Autoimmunity Laboratory, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, 16132 Genova (GE), Italy.

: Myasthenia gravis (MG) is a prototypical autoimmune disease, characterized by pathogenic autoantibodies targeting structures of the neuromuscular junction. Radioimmunoprecipitation assays (RIPAs) represent the gold standard for their detection. However, new methods are emerging to complement, or overcome RIPAs, also with the perspective of eliminating the use of radioactive reagents. Read More

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Participants' unspoken thoughts and feelings negatively influence the therapeutic alliance; a qualitative study in a multidisciplinary pain rehabilitation setting.

Disabil Rehabil 2021 May 10:1-11. Epub 2021 May 10.

Faculty of Medical Sciences, Section Health Psychology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.

Purpose: This study aimed to explore factors that influence participants' perceptions of the therapeutic alliance with healthcare professionals; their participation in the alliance; and their commitment to treatment in a multidisciplinary pain rehabilitation setting.

Materials And Methods: A qualitative research-design was used and 26 participants in a multidisciplinary pain rehabilitation program were interviewed in-depth.

Results: Initially, participants reported to be satisfied with their healthcare professionals. Read More

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[Imaging-based patient selection for mechanical thrombectomy based on time since symptom onset].

B Cheng G Broocks

Nervenarzt 2021 May 10. Epub 2021 May 10.

Klinik und Poliklinik für Neuroradiologische Diagnostik und Intervention, Universitätsklinikum Hamburg-Eppendorf, Martinistraße 52, 20251, Hamburg, Deutschland.

Background: In ischemic stroke due to large vessel occlusion (LVO), the indications for patient selection for endovascular mechanical thrombectomy (MT) are based on findings from brain imaging. Several imaging protocols from computed tomography (CT) or magnetic resonance imaging (MRI) are available to guide treatment decisions.

Objective: To recommend the optimal choice of imaging modalities and protocols for MT with respect to time windows from symptom onset. Read More

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Visual Exploration of Large Metabolic Models.

Bioinformatics 2021 May 10. Epub 2021 May 10.

Department of Computer and Information Science, University of Konstanz, Konstanz, Germany.

Motivation: Large metabolic models, including genome-scale metabolic models (GSMMs), are nowadays common in systems biology, biotechnology and pharmacology. They typically contain thousands of metabolites and reactions and therefore methods for their automatic visualisation and interactive exploration can facilitate a better understanding of these models.

Results: We developed a novel method for the visual exploration of large metabolic models and implemented it in LMME (Large Metabolic Model Explorer), an add-on for the biological network analysis tool VANTED. Read More

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Visit Content Analysis: Doctor-Patient Communication in Patients with Type 2 Diabetes.

Perm J 2021 May;25

Division of Research, Kaiser Permanente Northern California, Oakland, CA.

Introduction: The primary care visit is an important opportunity to discuss and modify diabetes management.

Objective: To gain insight into doctor-patient communication during primary care visits among English and Spanish speaking patients with type 2 diabetes and suboptimal glycemic control (HbA1c > 7%).

Methods: We conducted a quantitative content analysis of audiotaped primary care visits in 2 patient cohorts. Read More

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Review of multi-omics data resources and integrative analysis for human brain disorders.

Brief Funct Genomics 2021 May 8. Epub 2021 May 8.

Department of Biostatistics, Virginia Commonwealth University.

In the last decade, massive omics datasets have been generated for human brain research. It is evolving so fast that a timely update is urgently needed. In this review, we summarize the main multi-omics data resources for the human brains of both healthy controls and neuropsychiatric disorders, including schizophrenia, autism, bipolar disorder, Alzheimer's disease, Parkinson's disease, progressive supranuclear palsy, etc. Read More

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The Dynamic Entropy of Tumor Immune Infiltrates: The Impact of Recirculation, Antigen-Specific Interactions, and Retention on T Cells in Tumors.

Front Oncol 2021 22;11:653625. Epub 2021 Apr 22.

Earle A. Chiles Research Institute, Providence Cancer Institute, Providence Portland Medical Center, Portland, OR, United States.

Analysis of tumor infiltration using conventional methods reveals a snapshot view of lymphocyte interactions with the tumor environment. However, lymphocytes have the unique capacity for continued recirculation, exploring varied tissues for the presence of cognate antigens according to inflammatory triggers and chemokine gradients. We discuss the role of the inflammatory and cellular makeup of the tumor environment, as well as antigen expressed by cancer cells or cross-presented by stromal antigen presenting cells, on recirculation kinetics of T cells. Read More

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Acceleration of PDE-Based Biological Simulation Through the Development of Neural Network Metamodels.

Curr Pathobiol Rep 2020 Dec 6;8(4):121-131. Epub 2020 Nov 6.

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907.

Purpose Of Review: Partial differential equation (PDE) mathematical models of biological systems and the simulation approaches used to solve them are widely used to test hypotheses and infer regulatory interactions based on optimization of the PDE model against the observed data. In this review, we discuss the ability of powerful machine learning methods to accelerate the parametric screening of biophysical informed- PDE systems.

Recent Findings: A major shortcoming in more broad adaptation of PDE-based models is the high computational complexity required to solve and optimize the models and it requires many simulations to traverse the very high-dimensional parameter spaces during model calibration and inference tasks. Read More

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December 2020

Community Detection in Large-Scale Bipartite Biological Networks.

Front Genet 2021 21;12:649440. Epub 2021 Apr 21.

Centre for Molecular Medicine Norway, University of Oslo, Oslo, Norway.

Networks are useful tools to represent and analyze interactions on a large, or genome-wide scale and have therefore been widely used in biology. Many biological networks-such as those that represent regulatory interactions, drug-gene, or gene-disease associations-are of a bipartite nature, meaning they consist of two different types of nodes, with connections only forming between the different node sets. Analysis of such networks requires methodologies that are specifically designed to handle their bipartite nature. Read More

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Co-Isolation of Cytokines and Exosomes: Implications for Immunomodulation Studies.

Front Immunol 2021 19;12:638111. Epub 2021 Apr 19.

Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States.

Exosomes play a vital role in intercellular communication and their immunomodulatory potential have become an important focus in cancer research. Various methods have been developed for the isolation although each method differs in the number and purity of exosomes they yield. In melanoma, tumor-derived exosomes drive immunosuppression within the tumor microenvironment. Read More

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Strategies to Refine Gastric Stimulation and Pacing Protocols: Experimental and Modeling Approaches.

Front Neurosci 2021 22;15:645472. Epub 2021 Apr 22.

Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.

Gastric pacing and stimulation strategies were first proposed in the 1960s to treat motility disorders. However, there has been relatively limited clinical translation of these techniques. Experimental investigations have been critical in advancing our understanding of the control mechanisms that innervate gut function. Read More

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ECMO in cardiogenic shock and bridge to heart transplant.

Indian J Thorac Cardiovasc Surg 2021 Apr 11;37(Suppl 2):319-326. Epub 2020 Feb 11.

Department of Cardiothoracic Surgery, National Heart Centre Singapore, 5 Hospital drive, Singapore, 169609 Singapore.

Purpose: The aim of this review is to discuss the role of extracorporeal membrane oxygenation (ECMO) in cardiogenic shock and its use to bridge patients to heart transplantation.

Methods And Results: Consideration of published literature reveals indications for ECMO in cardiogenic shock and tools for patient selection, adequate evidence of its efficacy, its advantages when compared with other temporary mechanical circulatory support devices and details of its use as a bridge to decision, bridge to recovery, bridge to bridge (durable ventricular assist device) and bridge to heart transplant.

Conclusion: ECMO is invaluable in treating patients with medically refractory profound cardiogenic shock and allows for cardiac recovery or planning for permanent heart replacement treatments. Read More

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Diagnostic value of endometrial volume and flow parameters under 3D ultrasound acquisition in combination with serum CA125 in endometrial lesions.

Taiwan J Obstet Gynecol 2021 May;60(3):492-497

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China.

Objective: This study aims to discuss the differential diagnosis value of endometrial volume and flow parameters in combination with serum carbohydrate antigen 125 (CA125) in endometrial benign and malignant lesions.

Materials And Methods: The data of 250 patients with endometrial lesions were retrospectively analyzed. Carbohydrate antigen 125 (CA125) was determined before the operation. Read More

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How did individuals on Instagram discuss COVID-19 in the month following official pandemic status? A examination of user content.

J Prev Interv Community 2021 May 8:1-9. Epub 2021 May 8.

Department of Public Health, William Paterson University, Wayne, NJ, USA.

Objectives: Instagram is a popular social media site used by high numbers of young people. This study was designed to investigate COVID-19 content on Instagram during the month following official pandemic status from the World Health Organization (WHO).

Methods: A total of 200 posts were collected and analyzed over a four-week period between mid-February and mid-March 2020. Read More

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Challenges in Conducting Sexual Health and Violence Research in Older Adults Beyond the General Data Protection Regulation: A Belgian Case Study.

J Interpers Violence 2021 May 8:8862605211015256. Epub 2021 May 8.

International Centre for Reproductive Health, Department of Public Health and Primary Care, Ghent University, Ghent, Belgium.

Because of a growing older population, the sexual health (SH) of older adults, including sexual violence (SV), is becoming an increasingly important public health concern. Yet, reliable SV prevalence rates and risk factors are lacking, due to methodological shortcomings in current studies. SV research involves challenges regarding safety and disclosure, especially in older adults. Read More

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Physics-based reconstruction methods for magnetic resonance imaging.

Philos Trans A Math Phys Eng Sci 2021 Jun 10;379(2200):20200196. Epub 2021 May 10.

Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen, Germany.

Conventional magnetic resonance imaging (MRI) is hampered by long scan times and only qualitative image contrasts that prohibit a direct comparison between different systems. To address these limitations, model-based reconstructions explicitly model the physical laws that govern the MRI signal generation. By formulating image reconstruction as an inverse problem, quantitative maps of the underlying physical parameters can then be extracted directly from efficiently acquired k-space signals without intermediate image reconstruction-addressing both shortcomings of conventional MRI at the same time. Read More

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Social media and vision therapy: perspectives of providers and patients on Instagram.

J AAPOS 2021 May 6. Epub 2021 May 6.

Department of Ophthalmology, Byers Eye Institute, Stanford University, Palo Alto, California. Electronic address:

Purpose: To formally assess the content, intent, and authorship of the most popular Instagram hashtags related to pediatric ophthalmology.

Methods: Public Instagram posts with over 20 likes, using one or more of 6 vision therapy (VT) hashtags and containing English content were identified. A categorical classification system was used to analyze each post for the target audience: post owner, primary intent of post, diagnosis addressed, whether advice was provided, and whether references to studies were provided. Read More

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Evaluation of health care providers' use of the 'Exercise and Depression Toolkit': a case study.

BMC Psychiatry 2021 May 8;21(1):243. Epub 2021 May 8.

Department of Kinesiology, Faculty of Education, University of British Columbia, 210-6081 University Boulevard, Vancouver, BC, V6T 1Z1, Canada.

Background: Exercise is now recommended as a primary treatment for mild-moderate depression in Canada. The 'Exercise and Depression Toolkit' was developed to help health care providers (HCP) integrate these treatment guidelines into practice. The purpose of this study was to evaluate acceptability and perceived effectiveness of the toolkit in practice by HCPs working with individuals with depression. Read More

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Global prevalence and pathogenesis of headache in COVID-19: A systematic review and meta-analysis.

F1000Res 2020 12;9:1316. Epub 2020 Nov 12.

Medical Research Unit, School of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia.

: This study was conducted to determine the prevalence of headache in coronavirus disease 2019 (COVID-19) and to assess its association as a predictor for COVID-19. This study also aimed to discuss the possible pathogenesis of headache in COVID-19. : Available articles from PubMed, Scopus, and Web of Science were searched as of September 2 , 2020. Read More

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November 2020

Motor learning methods that induce high practice variability reduce kinematic and kinetic risk factors of non-contact ACL injury.

Hum Mov Sci 2021 May 6;78:102805. Epub 2021 May 6.

Centre for Health Sciences Research, School of Health & Society, University of Salford, UK.

The prevention of non-contact anterior cruciate ligament (ACL) injuries often involves movement training, but the effectiveness of different motor learning methods has not been fully investigated. The purpose of this study was therefore to examine the effects of linear pedagogy (LP), nonlinear pedagogy (NLP) and differential learning (DL) motor learning methods on changing kinetic and kinematic factors during expected sidestep cutting related to non-contact ACL injuries. These methods primarily differ in the amount and type of movement variability they induce during practice. Read More

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Recent advances in synthesis and applications of room temperature phosphorescence carbon dots.

Talanta 2021 Aug 31;231:122350. Epub 2021 Mar 31.

School of Chemistry and Chemical Engineering, and Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China. Electronic address:

Recently, room temperature phosphorescence (RTP) feature of carbon dots (CDs) has gradually diverted researchers' attention from fluorescence and sparks new research boom due to its ultra-long luminescence lifetime and large Stokes shift. Some attempts have been made to construct CDs-based RTP materials, and had seen some important progress. However, few review articles were published to systematically summarize them. Read More

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In-situ at a distance? challenges and opportunities for health and place research methods in a post-COVID-19 world.

Health Place 2021 May 6;69:102572. Epub 2021 May 6.

Centre for Healthy Community, School of Public Health, University of Alberta, Alberta, Canada.

In-situ methodologies, including go-along and photo-elicited interviews, are ideal for harnessing people's lived experiences of place and their meanings for health and health equity. Their immersive nature means that the COVID-19 pandemic has impacted their use. Physical distancing measures combined to anxiety over the sharing of physical space have created ethical and practical challenges to the conduct of in-person in-situ methodologies. Read More

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A method for measuring dynamic respiratory sinus arrhythmia (RSA) in infants and mothers.

Infant Behav Dev 2021 May 5;63:101569. Epub 2021 May 5.

Department of Psychological and Brain Sciences, Indiana University, United States.

The measurement of respiratory sinus arrythmia (RSA) in infants, children and adults is critical to the study of physiological regulation, and more recently, interpersonal physiological covariation, but it has been impeded by methods that limit its resolution to 30 s or longer. Recent analytical developments have suggested methods for studying dynamic RSA in adults, and we have extended this work to the study of infants and mothers. In the current paper, we describe a new analytical strategy for estimating RSA time series for infants and adults. Read More

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Methods and techniques for in vitro subcellular localization of radiopharmaceuticals and radionuclides.

Nucl Med Biol 2021 Apr 22;98-99:18-29. Epub 2021 Apr 22.

Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London, London SE1 7EH, United Kingdom. Electronic address:

In oncology, the holy grail of radiotherapy is specific radiation dose deposition in tumours with minimal healthy tissue toxicity. If used appropriately, injectable, systemic radionuclide therapies could meet these criteria, even for treatment of micrometastases and single circulating tumour cells. The clinical use of α and β particle-emitting molecular radionuclide therapies is rising, however clinical translation of Auger electron-emitting radionuclides is hampered by uncertainty around their exact subcellular localisation, which in turn affects the accuracy of dosimetry. Read More

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Cryo-EM: a new dawn in thyroid biology.

Mol Cell Endocrinol 2021 May 5:111309. Epub 2021 May 5.

University of Ljubljana, Faculty of Medicine, Institute of Biochemistry and Molecular Genetics, Vrazov trg 2, 1000, Ljubljana, Slovenia.

The thyroid gland accumulates the rare dietary element iodine and incorporates it into iodinated thyroid hormones, utilising several tightly regulated reactions and molecular mechanisms. Thyroid hormones are essential in vertebrates and play a central role in many biological processes, such as development, thermogenesis and growth. The control of these functions is exerted through the binding of hormones to nuclear thyroid hormone receptors that rule the transcription of numerous metabolic genes. Read More

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Health-Related Goal Setting and Achievement Among Veterans with High Technology Adoption.

J Gen Intern Med 2021 May 7. Epub 2021 May 7.

eHealth Partnered Evaluation Initiative, VA Bedford Healthcare System, Bedford, MA, USA.

Background: There is increasing recognition of the importance of supporting patients in their health-related goals. Patient-provider discussions and health-related mobile applications (apps) can support patients to pursue health goals; however, their impact on patient goal setting and achievement is not well understood.

Objective: To examine the relationships between the following: (1) patient demographics, patient-provider discussions, and health-related goal setting and achievement, and (2) patient mobile health app use and goal achievement. Read More

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The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort.

J Public Health (Oxf) 2021 May 8. Epub 2021 May 8.

Instituto de Virología Dr. J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina.

Background: To analyze the infectious extent of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) in different settings where prevention strategies are critical to limit infection spread, we evaluated SARS-COV-2 viability to guide public health policies regarding isolation criteria and infection control.

Methods: We attempted viral isolation in 82 nasopharyngeal swabs from 72 patients with confirmed SARS-COV-2 infection. Study population was divided into four groups: (i) Patients during the first week of symptoms; (ii) Patients with prolonged positive PCR; (iii) Healthcare workers from a hospital participating of an outbreak investigation, with SARS-COV-2 infection confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) and (iv) Recipients of convalescent immune plasma (CIP). Read More

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Implantable cardioverter defibrillator devices: when, how and who should discuss deactivation with patients: a systematic literature review.

BMJ Support Palliat Care 2021 May 7. Epub 2021 May 7.

Wolfson Palliative Care Research Centre, Hull York Medical School, University of Hull, Hull, UK.

Background: Implantable cardioverter defibrillators (ICDs) are used to treat life-threatening cardiac arrhythmias and prevent sudden cardiac arrest. As recipients age they may develop greater risk of dying as a result of progressive multimorbidity rather than sudden cardiac death. Defibrillation shocks may prolong an uncomfortable dying process. Read More

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[Pheochromocytomas and paragangliomas in pregnancy: about four cases and key messages on management].

Gynecol Obstet Fertil Senol 2021 May 4. Epub 2021 May 4.

CHU Lille, Clinique d'obstétrique, Univ. Lille, EA 2694 Metrics, Environnement périnatal et santé, F-59000 - Hôpital Jeanne de Flandre, avenue Eugène Avinée, 59000 Lille, France.

Objectifs: The diagnosis of a pheochromocytoma or paraganglioma secreting during pregnancy is a rare and serious situation, involving maternal-fetal prognosis. The purpose of this case series is to discuss the management of these patients.

Methods: This is a retrospective study of cases of pheochromocytoma (n=2) or paraganglioma (n=2) managed during pregnancy between 2013 and 2020 in one center (Lille, France). Read More

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Orbital Optimized Density Functional Theory for Electronic Excited States.

J Phys Chem Lett 2021 May 7:4517-4529. Epub 2021 May 7.

Kenneth S. Pitzer Center for Theoretical Chemistry, Department of Chemistry, University of California, Berkeley, California 94720, United States.

Density functional theory (DFT) based modeling of electronic excited states is of importance for investigation of the photophysical/photochemical properties and spectroscopic characterization of large systems. The widely used linear response time-dependent DFT (TDDFT) approach is, however, not effective at modeling many types of excited states, including (but not limited to) charge-transfer states, doubly excited states, and core-level excitations. In this perspective, we discuss state-specific orbital optimized (OO) DFT approaches as an alterative to TDDFT for electronic excited states. Read More

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