440,758 results match your criteria method tested


Computer-assisted UHPLC method development and optimization for the determination of albendazole and its related substances.

J Pharm Biomed Anal 2021 Jun 11;203:114203. Epub 2021 Jun 11.

George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Gh. Marinescu 38, RO-540139, Tîrgu Mureș, Romania; Sz-imfidum Ltd, RO-525401, Lunga No. 504, Romania. Electronic address:

Computer-aided ultrahigh performance liquid chromatographic (UHPLC) method development and optimization was undertaken in order to replace an underperforming European Pharmacopoeia method for the determination of albendazole and its related substances. In the preliminary screening, a temperature-gradient time bidimensional model was chosen to aid selection of the proper stationary phase. Hereinafter temperature-gradient time-ternary composition and temperature-gradient time-pH tridimensional models were applied for the optimization of critical method parameters. Read More

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Lack of active SARS-CoV-2 virus in a subset of PCR-positive COVID-19 congregate care patients.

J Clin Virol 2021 Jun 4;141:104879. Epub 2021 Jun 4.

Departments of Immunology and Microbial Disease, Albany Medical Center, Albany, NY, United States. Electronic address:

Highly sensitive nucleic acid amplification tests (NAATs) designed to detect SARS-CoV-2 RNA are the standard of care for the diagnosis of COVID-19. However, the accuracy of these methods for the quantitation of active virus rather than non-infectious RNA fragments that can persist for extended periods of time has been unclear. This issue is particularly relevant for congregate care patients who are unable to return to their home residence until fully negative by NAATs. Read More

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A facile approach synthesis of benzoylaryl benzimidazole as potential α-amylase and α-glucosidase inhibitor with antioxidant activity.

Bioorg Chem 2021 Jun 12;114:105073. Epub 2021 Jun 12.

Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 52571, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

Synthetic routes to a series of benzoylarylbenzimidazol 3a-h have been derived from 3,4-diaminobenzophenone and an appropriate arylaldehyde in the presence of ammonium chloride or a mixture of ammonium chloride and sodium metabisulfite as catalyst. The antioxidant activity of targeted compounds 3a-h has been measured by four different methods and the overall antioxidant evaluation of the compounds indicated the significant MCA, FRAP, and (DPPH-SA) of the compounds except for the compound 3h. In vitro antidiabetic assay of α-amylase and α-glucosidase suggest a good to excellent activity for most tested compounds. Read More

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Electric impedance spectroscopy feature extraction for tissue classification with electrode embedded surgical needles through a modified forward stepwise method.

Authors:
B Kent C Rossa

Comput Biol Med 2021 Jun 11;135:104522. Epub 2021 Jun 11.

Ontario Tech University, 2000 Simcoe Street North, Oshawa, ON L1G 0C5, Canada. Electronic address:

There has been a growing interest in developing electric impedance sensing surgical tools for tissue identification during surgery. A key facet of this development is identifying distinct features that can be used to identify tissues from one another. This paper explores several feature extraction techniques and classification methods applied to electric impedance data. Read More

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Ultra-short echo-time magnetic resonance imaging lung segmentation with under-Annotations and domain shift.

Med Image Anal 2021 Jun 2;72:102107. Epub 2021 Jun 2.

Division of Respirology, Department of Medicine, Western University, London, ON, N6A 5B7, Canada; Robarts Research Institute, School of Biomedical Engineering, Western University, London, ON, N6A 5B7, Canada. Electronic address:

Ultra-short echo-time (UTE) magnetic resonance imaging (MRI) provides enhanced visualization of pulmonary structural and functional abnormalities and has shown promise in phenotyping lung disease. Here, we describe the development and evaluation of a lung segmentation approach to facilitate UTE MRI methods for patient-based imaging. The proposed approach employs a k-means algorithm in kernel space for pair-wise feature clustering and imposes image domain continuous regularization, coined as continuous kernel k-means (CKKM). Read More

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An improved method for the isolation of amarogentin, the bitter principle of yellow gentian roots.

Food Chem 2021 Jun 16;364:130383. Epub 2021 Jun 16.

Dipartimento di Farmacia, Università "G. d'Annunzio" Chieti - Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy.

Amarogentin is well known to be among the most bitter naturally occurring compound. Either as an individual one or extracts, amarogentin is used as a food additive and as a dietary supplement. The aim of the present investigation is to set-up a convenient process to selectively isolate amarogentin from the ethanolic roots extract of Gentiana lutea. Read More

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Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats.

J Ethnopharmacol 2021 Jun 18:114348. Epub 2021 Jun 18.

Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, 100029, China. Electronic address:

Ethnopharmacological Relevance: Fructus Ligustri Lucidi (FLL) is an edible herb with anti-osteoporotic activity, yet whether and how the aqueous extract of this herb affect calcium metabolism in preservation of bone quality remain unclear.

Aim Of The Study: To investigate the effects of FLL aqueous extract on calcium balance and short-chain fatty acids (SCFAs) production in ovariectomized (OVX) rats.

Materials And Methods: OVX rats were daily and orally administrated with FLL aqueous extract (3. Read More

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PPARγ mediates the anti-pulmonary fibrosis effect of icaritin.

Toxicol Lett 2021 Jun 18. Epub 2021 Jun 18.

Xiangya Nursing School, Central South University, Changsha, Hunan, China. Electronic address:

Background: Pulmonary fibrosis is a fatal lung disease with limited treatment options. Icaritin is the active ingredient derived from the traditional Chinese medical plant Epimedium and possesses many biomedical activities. This study aimed to investigate the effects and molecular mechanisms of icaritin on bleomycin-induced pulmonary fibrosis in mice. Read More

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Do additional testing locations improve the detection of macular perimetric defects in glaucoma?

Ophthalmology 2021 Jun 18. Epub 2021 Jun 18.

City, University of London, Optometry and Visual Sciences, London, United Kingdom. Electronic address:

Purpose: to evaluate the ability of additional central testing locations to improve detection of macular visual field (VF) defects in glaucoma.

Design: prospective cross-sectional study.

Participants: 440 healthy people and 499 patients with Glaucomatous Optic Neuropathy (GON) were tested with a fundus tracked perimeter (CMP, CenterVue, Italy) using a 24-2 grid with 12 additional macular locations (24-2+). Read More

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Pesticide mixture effects on liver protein abundance in HepaRG cells.

Toxicology 2021 Jun 18:152839. Epub 2021 Jun 18.

NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany; SIGNATOPE GmbH, 72770 Reutlingen, Germany. Electronic address:

Toxicological effects of chemicals are mostly tested individually. However, consumers encounter exposure to complex mixtures, for example multiple pesticide residues, by consuming food such as crops, fruits or vegetables. Currently, more than 450 active substances are approved in the European Union, and there is little data on effects after combined exposure to several pesticides. Read More

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Long-term exposure to fine particulate matter, lung function and cognitive performance: A prospective Dutch cohort study on the underlying routes.

Environ Res 2021 Jun 18:111533. Epub 2021 Jun 18.

Institute of Sociology and Demography, University of Rostock, Rostock, Germany; German Center for Neurodegenerative Diseases, Bonn, Germany.

Background: Exposure to fine particulate matter and black carbon is related to cognitive impairment and poor lung function, but less is known about the routes taken by different types of air pollutants to affect cognition.

Objectives: We tested two possible routes of fine particulate matter (PM) and black carbon (BC) in impairing cognition, and evaluated their importance: a direct route over the olfactory nerve or the blood stream, and an indirect route over the lung.

Methods: We used longitudinal observational data for 49,705 people aged 18+ from 2006 to 2015 from the Dutch Lifelines cohort study. Read More

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Patterns of drinking in Aboriginal and Torres Strait Islander peoples as self-reported on the Grog Survey App: A representative urban and remote sample.

Drug Alcohol Rev 2021 Jun 21. Epub 2021 Jun 21.

The University of Sydney, Faculty of Medicine and Health, Discipline of Addiction Medicine, NHMRC Centre of Research Excellence in Indigenous Health and Alcohol, Sydney, Australia.

Introduction: Measuring self-reported alcohol use is challenging in any population, including when episodic drinking may be common. Drinking among Indigenous Australians has been shown to vary greatly within and between communities. However, most survey methods assume 'regular' patterns of drinking. Read More

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Leveraging social media and deep learning to detect rare megafauna in video surveys.

Conserv Biol 2021 Jun 21. Epub 2021 Jun 21.

MARBEC (Univ Montpellier, CNRS, Ifremer, IRD), place Eugène Bataillon, bât 24, CC093 34095, Montpellier, France.

Deep learning has become a key tool for the automated monitoring of animal populations with video-surveys. However, obtaining large amounts of images to train such models is a major challenge for rare and elusive species since field video-surveys provide few sightings. We propose a methodological framework that takes advantage of videos accumulated on social media for training deep learning models to detect rare charismatic species in the field. Read More

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Deep learning for classification of pediatric chest radiographs by WHO's standardized methodology.

PLoS One 2021 21;16(6):e0253239. Epub 2021 Jun 21.

Department of International Health, International Vaccine Access Center, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.

Background: The World Health Organization (WHO)-defined radiological pneumonia is a preferred endpoint in pneumococcal vaccine efficacy and effectiveness studies in children. Automating the WHO methodology may support more widespread application of this endpoint.

Methods: We trained a deep learning model to classify pneumonia CXRs in children using the World Health Organization (WHO)'s standardized methodology. Read More

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Stunting in pre-school and school-age children in the Peruvian highlands and its association with Fasciola infection and demographic factors.

PLoS Negl Trop Dis 2021 Jun 21;15(6):e0009519. Epub 2021 Jun 21.

Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, United States of America.

Fascioliasis is a zoonotic trematode infection that is endemic in the highlands of Peru. Chronic fascioliasis can be asymptomatic and remain undiagnosed for years. Chronic malnutrition in children, as manifested by stunting, leads to delayed cognitive development and lost productivity. Read More

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Evaluation of improved coloured targets to control riverine tsetse in East Africa: A Bayesian approach.

PLoS Negl Trop Dis 2021 Jun 21;15(6):e0009463. Epub 2021 Jun 21.

Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

Background: Riverine tsetse (Glossina spp.) transmit Trypanosoma brucei gambiense which causes Gambian Human African Trypanosomiasis. Tiny Targets were developed for cost-effective riverine tsetse control, and comprise panels of insecticide-treated blue polyester fabric and black net that attract and kill tsetse. Read More

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Different long-term avidity maturation for IgG anti-spike and anti-nucleocapsid SARS-CoV-2 in hospitalized COVID-19 patients.

Acta Clin Belg 2021 Jun 21:1-5. Epub 2021 Jun 21.

Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.

Introduction: The high variability of SARS-CoV-2 serological response after COVID-19 infection hampers its use as indicator of the timing of infection. A potential alternative method is the determination of affinity maturation of SARS-CoV-2 IgG, expressed as the SARS-CoV-2 IgG avidity.

Methods: SARS-CoV-2 IgG concentration and avidity were measured in sera of hospitalized COVID-19 patients sampled at two weeks and ≥12 weeks post symptom onset using an in-house developed protocol based on EUROIMMUN (anti-spike) and EDI™ (anti-nucleocapsid) SARS-CoV-2 IgG ELISA protocols. Read More

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Telehealth outpatient coronavirus disease 2019 case management at a tertiary hospital in Sydney.

J Telemed Telecare 2021 Jun 21:1357633X211024097. Epub 2021 Jun 21.

Department of Infectious Diseases, 6804Prince of Wales Hospital and Community Health Services, New South Wales, Australia214154.

Introduction: Coronavirus disease 2019 is an acute respiratory illness caused by severe acute respiratory syndrome coronavirus 2. The coronavirus disease 2019 pandemic upended the traditional paradigm of face-to-face provision of healthcare in the Australian context; as such, a telehealth model of active case management was implemented in our public health system, even though there was little supporting data for the safety of delivering patient care remotely to home-isolation patients in the setting of a highly infectious and potentially fatal illness.

Methods: A retrospective, single-centre, observational cohort study was performed over 6 weeks commencing 12 March 2020, including patients with coronavirus disease 2019 undergoing home isolation and being actively monitored by a coronavirus disease 2019 telephone assessment clinic. Read More

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Cardiovascular and cortisol responses to experimentally-induced minority stress.

Health Psychol 2021 May;40(5):316-325

Department of Psychology.

Objective: Lesbian, gay, and bisexual (LGB) individuals who report greater minority stress (e.g., discrimination) are at an elevated risk for multiple health problems. Read More

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Spin-Scaled Range-Separated Double-Hybrid Density Functional Theory for Excited States.

J Chem Theory Comput 2021 Jun 21. Epub 2021 Jun 21.

Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, P.O. Box 91, H-1521 Budapest, Hungary.

Our recently presented range-separated (RS) double-hybrid (DH) time-dependent density functional approach [ 927 (2021)] is combined with spin-scaling techniques. The proposed spin-component-scaled (SCS) and scaled-opposite-spin (SOS) variants are thoroughly tested for almost 500 excitations including the most challenging types. This comprehensive study provides useful information not only about the new approaches but also about the most prominent methods in the DH class. Read More

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Surgical Masks for Protection of Health Care Personnel Against Covid-19: Results from an Observational Study.

Clin Invest Med 2021 Jun 14;44(2):E48-54. Epub 2021 Jun 14.

Department of Respiratory and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, P. R. China; Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, P. R. China.

Purpose: The aim of the study was to describe the use of masks among health care personnel (HCP) exposed to index cases of coronavirus disease 2019 (COVID-19), and to evaluate any association with infection rate.

Methods: We did a retrospective, observational study of HCP at Zhongnan Hospital of Wuhan University for the management of COVID-19 (before person-to-person transmission was official confirmed, no additional protection was provided). A questionnaire was given to all staff listed on the roster in the clinical regions providing care for index patients with COVID-19. Read More

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Effect of a High-calorie Diet and Constant Light Exposure on Female Reproduction, Metabolism and Immune Inflammation: A Comparative Study of Different Mouse Models.

Am J Reprod Immunol 2021 Jun 21:e13479. Epub 2021 Jun 21.

Department of Gynecology, Hospital and Institute of Obstetrics and Gynecology, Shanghai Medical College, Fudan University, Shanghai, 200011, China.

Problem: Excess caloric intake and irregular circadian rhythm could severely impair female reproductive, metabolic and immune function. However, the similarities and differences between their individual and combined effects and mechanisms have not been fully elucidated. Due to limitations and confounding factors in clinical research, we used these two kinds of unhealthy factors to intervene the mice singly or in combination to explore their effects on individuals. Read More

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Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans.

J Genet Eng Biotechnol 2021 Jun 21;19(1):92. Epub 2021 Jun 21.

Molecular Genetics Laboratory, School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala, 673601, India.

Background: Candida albicans is an opportunistic human fungal pathogen responsible for superficial and systemic life-threatening infections. Treating these infections is challenging as many clinical isolates show increased drug resistance to antifungals. Chromosome (Chr) 4 monosomy was implicated in a fluconazole-resistant mutant. Read More

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Clinical Profile and Risk Factors for Severe Disease in 402 Children Hospitalized with SARS-CoV-2 from India: Collaborative Indian Pediatric COVID Study Group.

J Trop Pediatr 2021 Jun 21. Epub 2021 Jun 21.

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, India.

Introduction: There is a lack of large multicentric studies in children with COVID-19 from developing countries. We aimed to describe the clinical profile and risk factors for severe disease in children hospitalized with COVID-19 from India.

Methods: In this multicentric retrospective study, we retrieved data related to demographic details, clinical features, including the severity of disease, laboratory investigations, and outcome. Read More

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Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation.

J Water Health 2021 Jun;19(3):436-447

Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Avenida Trabalhador São-Carlense 400, São Carlos, São Paulo 13566-590, Brazil E-mail:

Giardia duodenalis and Cryptosporidium spp. are two of the most prominent aetiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. Read More

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Exploratory Outlier Detection for Acceleromyographic Neuromuscular Monitoring: Machine Learning Approach.

J Med Internet Res 2021 May 31;23(6):e25913. Epub 2021 May 31.

Department of Anesthesiology and Perioperative Medicine, Universitair Ziekenhuis Brussel, Brussel, Belgium.

Background: Perioperative quantitative monitoring of neuromuscular function in patients receiving neuromuscular blockers has become internationally recognized as an absolute and core necessity in modern anesthesia care. Because of their kinetic nature, artifactual recordings of acceleromyography-based neuromuscular monitoring devices are not unusual. These generate a great deal of cynicism among anesthesiologists, constituting an obstacle toward their widespread adoption. Read More

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A likelihood-based convolution approach to estimate major health events in longitudinal health records data: an external validation study.

J Am Med Inform Assoc 2021 Jun 21. Epub 2021 Jun 21.

Medical School and Institute for Health Informatics, University of Minnesota, Minneapolis, USA.

Objective: In electronic health record data, the exact time stamp of major health events, defined by significant physiologic or treatment changes, is often missing. We developed and externally validated a method that can accurately estimate these time stamps based on accurate time stamps of related data elements.

Materials And Methods: A novel convolution-based change detection methodology was developed and tested using data from the national deidentified clinical claims OptumLabs data warehouse, then externally validated on a single center dataset derived from the M Health Fairview system. Read More

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Comparison of Next-Generation Sequencing-Based Human Leukocyte Antigen Typing with Clinical Flow Cytometry and Allele-Specific PCR Melting Assays for HLA-B27 Genotyping.

J Appl Lab Med 2021 Jun 21. Epub 2021 Jun 21.

Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT.

Background: Due to the strong association between ankylosing spondylitis and Human Leukocyte Antigen (HLA)-B27, accurate identification of HLA-B27 is important in the diagnosis of patients with suspected spondyloarthritides. For this study, we compared a high-resolution HLA-B typing method to the clinical flow cytometry and allele-specific PCR melting assays to determine clinical benefits of high-resolution testing.

Methods: Residual clinical samples submitted for HLA-B27 testing by flow cytometry were tested by single-locus HLA-B genotyping using next-generation sequencing (NGS), and PCR with melting curve analysis, currently used as a reflex test for indeterminate flow cytometry results. Read More

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PBP4-mediated β-lactam resistance among clinical strains of Staphylococcus aureus.

J Antimicrob Chemother 2021 Jun 21. Epub 2021 Jun 21.

Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA.

Background: PBP4, a low-molecular-weight PBP in Staphylococcus aureus, is not considered to be a classical mediator of β-lactam resistance. Previous studies carried out by our group with laboratory strains of S. aureus demonstrated the ability of PBP4 to produce β-lactam resistance through mutations associated with the pbp4 promoter and/or gene. Read More

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The positive rates of hepatitis B surface antibody in youths after booster vaccination: a 4-year follow-up study with large sample.

Biosci Rep 2021 Jun 21. Epub 2021 Jun 21.

Sichuan University West China Hospital, Chengdu, China.

Background: Hepatitis B virus (HBV) infection is still a public issue of the world. Hepatitis B vaccination is widely used as an effective measure to prevent HBV infection. This large-sample study aimed to evaluate the positive rates of hepatitis B surface antibody (anti-HBs) in youths after booster vaccination. Read More

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