169,143 results match your criteria method selective

Hydrophilic silicon nanoparticles as a turn-off and colorimetric fluorescent probe for curcuminoids detection in food samples and cell imaging.

Food Chem 2021 Jul 19;366:130629. Epub 2021 Jul 19.

Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:

Hydrophilic fluorescent silicon nanoparticles (SiNPs) with good pH stability, salt-tolerance and anti-photobleaching were for the first time prepared from hydrophobic 3-glycidoxypropyltrimethoxysilane. Employing SiNPs as the fluorescence probe, selective quantification of curcuminoids based on the quenching effect was realized with a linearity of 0.046-7. Read More

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Autocrine regulation of wound healing by ATP release and P2Y receptor activation.

Life Sci 2021 Jul 24:119850. Epub 2021 Jul 24.

Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address:

Aims: Application of exogenous nucleotides can modulate wound healing via the activation of purinergic receptors. However, evidence for the release of endogenous nucleotides and the subsequent activation of purinergic receptors in this process has not been well defined. Therefore, the current study aimed to investigate wound-mediated nucleotide release and autocrine purinergic signalling during HaCaT keratinocyte wound closure following scratch injury. Read More

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Radiosynthesis and in silico bioevaluation of I-Sulfasalazine as a highly selective radiotracer for imaging of ulcerative colitis.

Chem Biol Drug Des 2021 Jul 27. Epub 2021 Jul 27.

Department of Pharmaceutical Chemistry, Ahram Canadian University, Giza-12511, Egypt.

This study demonstrated the tracking of ulcerative colitis, which is considered a stressful immune disease. Although there are many ways to test for this disease including dependence on gases, dyes, and painful anal endoscopy, these treatment modalities have many disadvantages. Hence, it is the utmost need of time to discover new methods to detect this chronic immune disease and to avoid the defects of traditional methodologies. Read More

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Glycan Labeling and Analysis in Cells and In Vivo.

Annu Rev Anal Chem (Palo Alto Calif) 2021 Jun;14(1):363-387

College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China; email:

As one of the major types of biomacromolecules in the cell, glycans play essential functional roles in various biological processes. Compared with proteins and nucleic acids, the analysis of glycans in situ has been more challenging. Herein we review recent advances in the development of methods and strategies for labeling, imaging, and profiling of glycans in cells and in vivo. Read More

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Lewis Basic Salt-Promoted Organosilane Coupling Reactions with Aromatic Electrophiles.

J Am Chem Soc 2021 Jul 27. Epub 2021 Jul 27.

Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States.

Lewis basic salts promote benzyltrimethylsilane coupling with (hetero)aryl nitriles, sulfones, and chlorides as a new route to 1,1-diarylalkanes. This method combines the substrate modularity and selectivity characteristic of cross-coupling with the practicality of a base-promoted protocol. In addition, a Lewis base strategy enables a complementary scope to existing methods, employs stable and easily prepared organosilanes, and achieves selective arylation in the presence of acidic functional groups. Read More

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Reconsidering the role of selective sodium channel blockers in genetic generalized epilepsy.

Acta Neurol Scand 2021 Jul 27. Epub 2021 Jul 27.

Epilepsy Unit, Department of Human Neurosciences, Policlinico Umberto I, Sapienza University of Rome, Italy.

Objective: Selective sodium channel blockers (SSCBs) have a limited use in genetic generalized epilepsy (GGE), due to their well-known risk of seizure worsening. Although their therapeutic potential in GGE has been suggested by recent evidence, electro-clinical data supporting their prescription are lacking. We aimed to investigate SSCB safety and effectiveness in a GGE cohort. Read More

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Prevalence of Listeria monocytogenes fecal carriers in HIV-infected and -uninfected pregnant women from Brazil.

Braz J Microbiol 2021 Jul 27. Epub 2021 Jul 27.

Department Preventive Medicine and Instituto de Puericultura E Pediatria Martagão Gesteira, Instituto de Puericultura e Pediatria Martagão Gesteira - UFRJ, R Bruno Lobo, 50 - Ilha do Fundão, Rio de Janeiro, Brazil.

Objective: The aim of this study is to describe the prevalence of Listeria spp. in feces of HIV-infected and -uninfected pregnant women in Brazil.

Methods: Cross-sectional study. Read More

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LINC00667 Promotes Progression of Esophageal Cancer Cells by Regulating miR-200b-3p/SLC2A3 Axis.

Dig Dis Sci 2021 Jul 27. Epub 2021 Jul 27.

Department of Gastroenterology, Taishan Hospital, Taian, Shandong Province, China.

Background: Recently, more and more evidence indicated that the long non-coding RNA was strictly related to the occurrence and progression of human cancers, including esophageal cancer (EC). We observed that LINC00667 was increased in EC, but the function of LINC00667 was unclear. Therefore, the function and potential molecular mechanism of LINC00667 in the progression of EC need to be further studied. Read More

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Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant.

Plant Cell Rep 2021 Jul 27. Epub 2021 Jul 27.

Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA.

Key Message: We have defined the conditions for citrus transformations using glyphosate as selection agent. This protocol results in high transformation rate and low incidence of chimeric shoots. Glyphosate, the most widely used herbicide in the world, specifically inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), an essential enzyme of the shikimate pathway. Read More

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Acute alcohol intoxication and the cocktail party problem: do "mocktails" help or hinder?

Psychopharmacology (Berl) 2021 Jul 27. Epub 2021 Jul 27.

School of Psychology & Clinical Language Sciences, University of Reading, RG6 6AL, Reading, UK.

Rationale: To test the notion that alcohol impairs auditory attentional control by reducing the listener's cognitive capacity.

Objectives: We examined the effect of alcohol consumption and working memory span on dichotic speech shadowing and the cocktail party effect-the ability to focus on one of many simultaneous speakers yet still detect mention of one's name amidst the background speech. Alcohol was expected either to increase name detection, by weakening the inhibition of irrelevant speech, or reduce name detection, by restricting auditory attention on to the primary input channel. Read More

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Development and Validation of a Quantitative UHPLC-MS/MS Method for the Determination of Alpha-Chloralose in Feline Blood and Application on Blood Samples Collected From Cats With Symptoms of Alpha-Chloralose Poisoning.

J Anal Toxicol 2021 Jul 27. Epub 2021 Jul 27.

Swedish National Veterinary Institute (SVA), 75189 Uppsala, Sweden.

Alpha-chloralose (AC) is used as a rodenticide as well as an anaesthetic agent in laboratory animals. It was previously also used as an avicide. Detection of AC in blood samples or post-mortem in body tissues is key for diagnosis of clinical cases and a requirement for surveillance of secondary toxicosis, including potential cases in wild animals. Read More

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Systematic Review and Meta-analysis of Outcome after Mild Traumatic Brain Injury in Older People.

J Int Neuropsychol Soc 2021 Jul 27:1-20. Epub 2021 Jul 27.

Department of Psychology, Caulfield Hospital, Caulfield, Australia.

Objective: Older age is often identified as a risk factor for poor outcome from traumatic brain injury (TBI). However, this relates predominantly to mortality following moderate-severe TBI. It remains unclear whether increasing age exerts risk on the expected recovery from mild TBI (mTBI). Read More

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Partial depletion and repopulation of microglia have different effects in the acute MPTP mouse model of Parkinson's disease.

Cell Prolif 2021 Jul 26:e13094. Epub 2021 Jul 26.

Department of Translational Neuroscience, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Jing' an District Centre Hospital of Shanghai Institutes of Brain Science, Fudan University, Shanghai, China.

Objectives: Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive and selective degeneration of dopaminergic neurons. Microglial activation and neuroinflammation are associated with the pathogenesis of PD. However, the relationship between microglial activation and PD pathology remains to be explored. Read More

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2-Phenyl-3-(phenylselanyl)benzofuran elicits acute antidepressant-like action in male Swiss mice mediated by modulation of the dopaminergic system and reveals therapeutic efficacy in both sexes.

Psychopharmacology (Berl) 2021 Jul 27. Epub 2021 Jul 27.

Programa de Pós-Graduação em Bioquímica e Bioprospecção (PPGBBio), Laboratório de Bioquímica e Neurofarmacologia Molecular (LABIONEM), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ciências Químicas, Farmacêuticas e de Alimentos (CCQFA), Universidade Federal de Pelotas (UFPel), Pelotas, RS, CEP 96010-900, Brasil.

Rationale: Depression is a psychiatric disorder that constitutes one of the leading causes of disability worldwide. 2-Phenyl-3-(phenylselanyl)benzofuran (SeBZF1) has been studied as a potential antidepressant drug, but its pharmacological action needs more investigation.

Objectives And Methods: Our aim was to extend information about the antidepressant-like action of SeBZF1 using the mouse tail suspension test (TST). Read More

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An electrostatically regulated organic self-assembly for rapid and sensitive detection of heparin in serum.

Anal Methods 2021 Jul 27. Epub 2021 Jul 27.

Key Laboratory of Public Health Safety of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Public Health, Hebei University, Baoding 071002, P. R. China.

Heparin (Hep) is a highly negatively charged linear glycosaminoglycan involved in various physiological processes, especially blood coagulation. Hep is also a first-line drug for anticoagulation and prevention of thromboembolism, but its overdose will cause serious side effects. Herein, we designed a long-wavelength double-charged cationic fluorescent probe PYPN, and studied its aggregation state and detection performance for Hep. Read More

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Valley-selective optical Stark effect of exciton-polaritons in a monolayer semiconductor.

Nat Commun 2021 Jul 26;12(1):4530. Epub 2021 Jul 26.

Applied Physics Program, Northwestern University, Evanston, IL, 60208, USA.

Selective breaking of degenerate energy levels is a well-known tool for coherent manipulation of spin states. Though most simply achieved with magnetic fields, polarization-sensitive optical methods provide high-speed alternatives. Exploiting the optical selection rules of transition metal dichalcogenide monolayers, the optical Stark effect allows for ultrafast manipulation of valley-coherent excitons. Read More

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Generation of non-stabilized alkyl radicals from thianthrenium salts for C-B and C-C bond formation.

Nat Commun 2021 Jul 26;12(1):4526. Epub 2021 Jul 26.

State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.

Sulfonium salts bearing a positively charged sulfur atom with three organic substituents have intrigued chemists for more than a century for their unusual structures and high chemical reactivity. These compounds are known to undergo facile single-electron reduction to emerge as a valuable and alternative source of aryl radicals for organic synthesis. However, the generation of non-stabilized alkyl radicals from sulfonium salts has been a challenge for several decades. Read More

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Monitoring autophagy-dependent ferroptosis.

Methods Cell Biol 2021 18;165:163-176. Epub 2020 Nov 18.

Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, United States. Electronic address:

Ferroptosis is an iron-dependent form of regulated cell death, driven by the accumulation of lipid peroxidation. Autophagy is a lysosome-dependent degradation process that can be used to remove and recover intracellular components, such as dysfunctional proteins and damaged organelles. By regulating iron storage and oxidative stress, excessive autophagy is involved in the induction and execution of ferroptosis. Read More

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November 2020

Monitoring selective autophagy of mitochondria using super-resolution microscopy.

Methods Cell Biol 2021 18;165:153-161. Epub 2020 Nov 18.

Signaling Programme, Babraham Institute, Cambridge, United Kingdom. Electronic address:

Selective elimination of damaged mitochondria via macroautophagy (mitophagy) is a conserved cellular process that plays an important role in organismal health. In recent years mitophagy has been studied in parallel to the more general, non-selective autophagy pathway induced in response to amino acid starvation with important similarities and differences noted between the two. The elaborate sequence of membrane rearrangements that give rise to autophagosomes in the non-selective pathway have their counterpart in mitophagy, but with the addition of other factors, such as a ubiquitin mark and mitophagy receptors, which mediate cargo recognition. Read More

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November 2020

Exploring selective autophagy in Drosophila: Methods to identify Atg8-interacting proteins.

Methods Cell Biol 2021 18;165:13-29. Epub 2020 Nov 18.

School of Life Sciences, University of Warwick, Coventry, United Kingdom. Electronic address:

Autophagy has been described as a catabolic process in which cytoplasmic material is being recycled under various conditions of cellular stress, preventing cell damage and promoting cell survival. Drosophila has been demonstrated to provide an excellent animal model for the study of autophagy. Here, we provide a detailed experimental procedure for the identification of Atg8a interactors, exploiting the iLIR database, followed by the in vitro confirmation of interactions and in situ detection of the respective proteins. Read More

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November 2020

Incidence of major complications from embolo-sclerotherapy of head and neck vascular malformations in a single specialist centre.

Vascular 2021 Jul 26:17085381211035279. Epub 2021 Jul 26.

Department of Vascular Surgery, 4965Royal Free London NHS Foundation Trust, London, UK.

Objective: Current data on the nature and rate of major complications for embolo-sclerotherapy (EST) of vascular malformations are scarce. However, even fewer studies focus on vascular malformations specific to the head and neck, which confer an increased specific risk of airway compromise, neurologic and ophthalmologic injury. More understanding is required surrounding the type and incidence of complications to improve treatment planning and informed consent. Read More

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Mechanistic Insights into the Electrochemical Reduction of CO and N on the Regulation of a Boron Nitride Defect-Derived Two-Dimensional Catalyst using Density Functional Theory Calculations.

J Phys Chem Lett 2021 Jul 23:7151-7158. Epub 2021 Jul 23.

School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China.

Electrochemical reductions of CO (ECRR) and N (ENRR) can not only reduce CO emissions in the air but also make use of N and HO, the most extensive resources on earth, to produce high value-added chemicals, which has become one of the hot research directions. In this study, the formation energy () and dissolution potential () of 96 two-dimensional catalysts derived from different defect sites of monoclinic crystal boron nitride (BN) were calculated, and the catalysts with thermodynamic and electrochemical stability were selected. The suitable catalysts for producing HCOOH (Ga/[email protected]), CO ([email protected]), and CHOH ([email protected]) by ECRR and NH ([email protected]) by ENRR were predicated based on a selective calculation method. Read More

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Lipid-Coated MCM-41 Mesoporous Silica Nanoparticles Loaded with Berberine Improved Inhibition of Acetylcholine Esterase and Amyloid Formation.

ACS Biomater Sci Eng 2021 Jul 23. Epub 2021 Jul 23.

Centre of Experimental Medicine & Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.

Selective permeability of the blood-brain barrier limits effective treatment of neurodegenerative disorders. In the present study, brain-targeted lipid-coated mesoporous silica nanoparticles (MSNs) containing berberine (BBR) were synthesized for the effective treatment of Alzheimer's disease (AD). The study involved synthesis of Mobil Composition of Matter-41 (MCM-41) mesoporous silica nanoparticles (MSNs), BBR loading, and lipid coating of MSNs (MSNs-BBR-L) and and characterization of MSNs-BBR-L. Read More

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Selective blood-brain barrier permeabilisation of brain metastases by a type-1 receptor selective tumour necrosis factor mutein.

Neuro Oncol 2021 Jul 23. Epub 2021 Jul 23.

Cancer Research UK and Medical Research Council Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, UK.

Background: Metastasis to the brain is a major challenge with poor prognosis. The blood-brain barrier (BBB) is a significant impediment to effective treatment, being intact during the early stages of tumour development and heterogeneously permeable at later stages. Intravenous injection of tumour necrosis factor (TNF) selectively induces BBB permeabilisation at sites of brain micrometastasis, in a TNF type-1 receptor (TNFR1) dependent manner. Read More

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Spontaneous conversion of prenyl halides to acids: application in metal-free preparation of deuterated compounds under mild conditions.

Org Biomol Chem 2021 Jul 23. Epub 2021 Jul 23.

Chulabhorn Graduate Institute, Chemical Biology Program, Chulabhorn Royal Academy, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand. and Chulabhorn Research Institute, Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand and Center of Excellence on Environmental Health and Toxicology (EHT), CHE, Ministry of Education, Bangkok, Thailand.

Here we reveal a simple generation of deuterium halide (DX) from common and inexpensive reagents readily available in a synthetic chemistry laboratory, i.e. prenyl-, allyl-, and propargyl halides, under mild conditions. Read More

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Enzymatic Asymmetric Reduction of Unfunctionalized C=C Bonds with Archaeal Geranylgeranyl Reductases.

Chembiochem 2021 Jul 22. Epub 2021 Jul 22.

Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Albertstrasse 25, 79104, Freiburg, Germany.

The asymmetric reduction of activated C=C bonds such as enones is well established for non-enzymatic methods as well as in biocatalysis. However, the asymmetric reduction of unfunctionalized C=C bonds is mainly performed with transition metal catalysts whereas biocatalytic approaches are lacking. We have tested two FAD-dependent archaeal geranylgeranyl reductases (GGR) for the asymmetric reduction of isolated C=C bonds. Read More

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Application of tandem fast protein liquid chromatography to purify intact native monomeric/aggregated Tamm-Horsfall protein from human urine and systematic comparisons with diatomaceous earth adsorption and salt precipitation: yield, purity and time-consumption.

Anal Methods 2021 Jul 23. Epub 2021 Jul 23.

Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, 6th Floor - SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand.

Tamm-Horsfall protein (THP) is a high-abundance urinary protein. Although its functions have been studied for years, several aspects of these remain unclear. To achieve more knowledge on THP functions, an effective isolation/purification method providing a high yield and high purity is required. Read More

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Postbariatric hypoglycemia: symptom patterns and associated risk factors in the Longitudinal Assessment of Bariatric Surgery study.

Surg Obes Relat Dis 2021 May 19. Epub 2021 May 19.

Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.

Background: Postbariatric hypoglycemia (PBH) can be a devastating complication for which current therapies are often incompletely effective. More information is needed regarding frequency, incidence, and risk factors for PBH.

Objectives: To examine hypoglycemia symptoms following Roux-en-Y gastric bypass (RYGB) and laparoscopic adjustable gastric banding (LAGB) and baseline and in-study risk factors. Read More

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An assessment of the passivity of the fit of multiunit screw-retained implant frameworks manufactured by using additive and subtractive technologies.

J Prosthet Dent 2021 Jul 19. Epub 2021 Jul 19.

Assistant Professor, Department of Prosthetic Dentistry, Faculty of Dentistry, Near East University, Nicosia, Turkey.

Statement Of Problem: The lack of passive fit in implant-supported restorations can lead to mechanical and biological complications and compromise the longevity of the prosthesis. The manufacturing technique and evaluation site are factors that may affect the passive fit of multiunit screw-retained implant frameworks. However, scientific information regarding this issue is lacking. Read More

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Adhesion of veneering porcelain to cobalt-chromium dental alloys processed with casting, milling, and additive manufacturing methods: A systematic review and meta-analysis.

J Prosthet Dent 2021 Jul 19. Epub 2021 Jul 19.

Professor and Head, Division of Dental Biomaterials, Clinic for Reconstructive Dentistry, Center for Dental and Oral Medicine, University of Zürich, Zurich, Switzerland.

Statement Of Problem: Selective laser melting (SLM) additive manufacturing (AM) technologies provide an alternative to conventional casting and milling procedures in fabricating metal-ceramic dental prostheses. However, the quality of porcelain bond strength to the SLM AM cobalt-chromium (Co-Cr) metal framework of a dental restoration is unclear.

Purpose: The purpose of this systematic review and meta-analysis was to identify in vitro studies that reported the porcelain bond strength to SLM AM Co-Cr dental metal alloys and compare the porcelain bond strength values to cast, milled, and additively manufactured Co-Cr dental alloys. Read More

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