101 results match your criteria metazoan caspases

Ancestral role of TNF-R pathway in cell differentiation in the basal metazoan .

J Cell Sci 2021 01 25;134(2). Epub 2021 Jan 25.

Ludwig-Maximilians-Universität München, Department Biologie II, Groβhaderner Str. 2, 82152 Planegg-Martinsried, Munich, Germany

Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activate signalling cascades. Read More

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January 2021

Maize metacaspases modulate the defense response mediated by the NLR protein Rp1-D21 likely by affecting its subcellular localization.

Plant J 2021 01 20;105(1):151-166. Epub 2020 Nov 20.

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, Shandong, 266237, PR China.

Plants usually employ resistance (R) genes to defend against the infection of pathogens, and most R genes encode intracellular nucleotide-binding, leucine-rich repeat (NLR) proteins. The recognition between R proteins and their cognate pathogens often triggers a rapid localized cell death at the pathogen infection sites, termed the hypersensitive response (HR). Metacaspases (MCs) belong to a cysteine protease family, structurally related to metazoan caspases. Read More

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January 2021

Non-lethal message from the Holy Land: The first international conference on nonapoptotic roles of apoptotic proteins.

FEBS J 2021 04 21;288(7):2166-2183. Epub 2020 Sep 21.

Pharmacology and Therapeutics, Biomedical Sciences, Dangan, NUI Galway, Ireland.

Apoptosis is a major form of programmed cell death (PCD) that eliminates unnecessary and potentially dangerous cells in all metazoan organisms, thus ensuring tissue homeostasis and many developmental processes. Accordingly, defects in the activation of the apoptotic pathway often pave the way to disease. After several decades of intensive research, the molecular details controlling the apoptosis program have largely been unraveled, as well as the regulatory mechanisms of caspase activation during apoptosis. Read More

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Intertwined Functions of Separase and Caspase in Cell Division and Programmed Cell Death.

Sci Rep 2020 04 9;10(1):6159. Epub 2020 Apr 9.

Department of Molecular, Cellular, and Developmental Biology, and Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, 93106, USA.

Timely sister chromatid separation, promoted by separase, is essential for faithful chromosome segregation. Separase is a member of the CD clan of cysteine proteases, which also includes the pro-apoptotic enzymes known as caspases. We report a role for the C. Read More

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Unraveling oxidative stress response in the cestode parasite Echinococcus granulosus.

Sci Rep 2019 11 4;9(1):15876. Epub 2019 Nov 4.

Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre, Brazil.

Cystic hydatid disease (CHD) is a worldwide neglected zoonotic disease caused by Echinococcus granulosus. The parasite is well adapted to its host by producing protective molecules that modulate host immune response. An unexplored issue associated with the parasite's persistence in its host is how the organism can survive the oxidative stress resulting from parasite endogenous metabolism and host defenses. Read More

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November 2019

The Proteasome as a Drug Target in the Metazoan Pathogen, .

ACS Infect Dis 2019 10 12;5(10):1802-1812. Epub 2019 Aug 12.

Center for Discovery and Innovation in Parasitic Diseases , University of California, San Diego , 9500 Gilman Drive , La Jolla , California 92093 , United States.

Proteases are fundamental to successful parasitism, including that of the schistosome flatworm parasite, which causes the disease schistosomiasis in 200 million people worldwide. The proteasome is receiving attention as a potential drug target for treatment of a variety of infectious parasitic diseases, but it has been understudied in the schistosome. Adult were incubated with 1 μM concentrations of the proteasome inhibitors bortezomib, carfilzomib, and MG132. Read More

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October 2019

The Challenge of the Sponge (Olivi, 1792) in the Presence of a Symbiotic Bacterium and a Pathogen Bacterium.

Genes (Basel) 2019 06 26;10(7). Epub 2019 Jun 26.

Laboratoire de Biotechnologie et de Chimie Marines, Université de Bretagne Sud, EA 3884-IUEM, 56100 Lorient, France.

Sponges, which are in close contact with numerous bacteria in prey/predator, symbiotic and pathogenic relationships, must provide an appropriate response in such situations. This starts with a discriminating recognition of the partner either by a physical contact or through secreted molecules or both. We investigated the expression of the Toll-like receptor, Caspase 3/7, Tumor Necrosis Factor receptor-associated factor 6, Bcl-2 homology protein-2 and macrophage expressed genes of axenic sponge cells in the presence of a symbiotic bacterium ( sp. Read More

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Evolution and structural diversity of metacaspases.

J Exp Bot 2019 04;70(7):2039-2047

Department of Chemistry, Umeå University, Umeå, Sweden.

Caspases are metazoan proteases, best known for their involvement in programmed cell death in animals. In higher plants genetically controlled mechanisms leading to the selective death of individual cells also involve the regulated interplay of various types of proteases. Some of these enzymes are structurally homologous to caspases and have therefore been termed metacaspases. Read More

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Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts by Legionella pneumophila.

Cell Microbiol 2019 01 15;21(1):e12971. Epub 2018 Nov 15.

Department of Microbiology and Immunology, College of Medicine, University of Louisville, Louisville, Kentucky.

Legionella pneumophila is a ubiquitous environmental bacterium that has evolved to infect and proliferate within amoebae and other protists. It is thought that accidental inhalation of contaminated water particles by humans is what has enabled this pathogen to proliferate within alveolar macrophages and cause pneumonia. However, the highly evolved macrophages are equipped with more sophisticated innate defence mechanisms than are protists, such as the evolution of phagotrophic feeding into phagocytosis with more evolved innate defence processes. Read More

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January 2019

Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it.

PLoS One 2018 31;13(1):e0190548. Epub 2018 Jan 31.

Department of Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana State, India.

Argonaute family proteins are well conserved among all organisms. Its role in mitotic cell cycle progression and apoptotic cell elimination is poorly understood. Earlier we have established the contribution of Ago-1 in cell cycle control related to G2/M cyclin in Drosophila. Read More

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February 2018

DNA-damage inducible protein 1 is a conserved metacaspase substrate that is cleaved and further destabilized in yeast under specific metabolic conditions.

FEBS J 2018 03 3;285(6):1097-1110. Epub 2018 Feb 3.

Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

Metacaspases, distant relatives of metazoan caspases, have been shown to participate in programmed cell death in plants and in progression of the cell cycle and removal of protein aggregates in unicellular eukaryotes. However, since natural proteolytic substrates have scarcely been identified to date, their roles in these processes remain unclear. Here, we report that the DNA-damage inducible protein 1 (Ddi1) represents a conserved protein substrate for metacaspases belonging to divergent unicellular eukaryotes (trypanosomes and yeasts). Read More

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Caspase signaling, a conserved inductive cue for metazoan cell differentiation.

Semin Cell Dev Biol 2018 10 11;82:96-104. Epub 2017 Nov 11.

Regenerative Medicine Program, Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, The Ottawa Hospital, Ottawa, Ontario, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada; Department of Medicine, Division of Cardiology, University of Ottawa, Ottawa, Ontario, Canada. Electronic address:

Caspase signaling pathways were originally discovered as conveyors of programmed cell death, yet a compendium of research over the past two decades have demonstrated that these same conduits have a plethora of physiologic functions. Arguably the most extensive non-death activity that has been attributed to this protease clade is the capacity to induce cell differentiation. Caspase control of differentiation is conserved across diverse metazoan organisms from flies to humans, suggesting an ancient origin for this form of cell fate control. Read More

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October 2018

Structural and functional diversity of caspase homologues in non-metazoan organisms.

Protoplasma 2018 Jan 25;255(1):387-397. Epub 2017 Jul 25.

Department of Chemistry, Umeå University, 901 87, Umeå, Sweden.

Caspases, the proteases involved in initiation and execution of metazoan programmed cell death, are only present in animals, while their structural homologues can be found in all domains of life, spanning from simple prokaryotes (orthocaspases) to yeast and plants (metacaspases). All members of this wide protease family contain the p20 domain, which harbours the catalytic dyad formed by the two amino acid residues, histidine and cysteine. Despite the high structural similarity of the p20 domain, metacaspases and orthocaspases were found to exhibit different substrate specificities than caspases. Read More

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January 2018

Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

Front Microbiol 2016 6;7:1043. Epub 2016 Jul 6.

Science for Life Laboratory, Department of Ecology, Environment and Plant Sciences, Stockholm University Solna, Sweden.

Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural setting. We here present data from the first microbial community-wide metacaspase survey; performed by querying metagenomic and metatranscriptomic datasets from the brackish Baltic Sea, a water body characterized by pronounced environmental gradients and periods of massive cyanobacterial blooms. Read More

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Modeling Metazoan Apoptotic Pathways in Yeast.

Methods Mol Biol 2016 ;1419:161-83

Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Melbourne, VIC, 3083, Australia.

This chapter describes techniques for characterizing metazoan apoptotic pathways using Saccharomyces cerevisiae. Active forms of the major apoptotic effectors-caspases, Bax and Bak-are all lethal to yeast. Using this lethality as a readout of caspase/Bax/Bak activity, proteins and small molecules that directly or indirectly regulate the activity of these effectors can be investigated in yeast, and apoptotic inhibitors can be identified using functional yeast-based screens. Read More

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December 2017

[Research on the Tomato Metacaspase Protein Interactions with Ca2+ by Spectroscopy and Molecular Probe].

Guang Pu Xue Yu Guang Pu Fen Xi 2015 Jun;35(6):1643-8

Metacaspases are cysteine-dependent proteases found in protozoa, fungi and plants and are distantly related to metazoan caspases. Most of MCPs activation are the calcium dependent, but the mechanisms are still unknown. Based on the techniques of CD spectroscopy, fluorescence spectroscopy, and Terbium Stains-all probe, we selected three purified recombinant proteins from key residues mutated in tomato metacaspase (LeMCA1), including conserved catalytic site (C139A) mutant, N-sequenced cleaved site (K223G) mutant and the predicted Ca2+ binding sites (D116A/D117A) mutant, to explore the interaction mechanism of LeMCA1 and Ca2+. Read More

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MALT1 is not alone after all: identification of novel paracaspases.

Cell Mol Life Sci 2016 Mar 16;73(5):1103-16. Epub 2015 Sep 16.

Inflammation Research Center, VIB, Ghent, Belgium.

Paracaspases and metacaspases are two families of caspase-like proteins identified in 2000. Up until now paracaspases were considered a single gene family with one known non-metazoan paracaspase in the slime mold Dictyostelium and a single animal paracaspase called MALT1. Human MALT1 is a critical signaling component in many innate and adaptive immunity pathways that drive inflammation, and when it is overly active, it can also cause certain forms of cancer. Read More

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Orthocaspases are proteolytically active prokaryotic caspase homologues: the case of Microcystis aeruginosa.

Mol Microbiol 2015 Oct 17;98(1):142-50. Epub 2015 Jul 17.

Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000, Ljubljana, Slovenia.

Caspases are a family of cysteine-dependent proteases known to be involved in the process of programmed cell death in metazoans. Recently, cyanobacteria were also found to contain caspase-like proteins, but their existence has only been identified in silico up to now. Here, we present the first experimental characterisation of a prokaryotic caspase homologue. Read More

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October 2015

Targeting the extrinsic apoptotic pathway in cancer: lessons learned and future directions.

Avi Ashkenazi

J Clin Invest 2015 Feb 2;125(2):487-9. Epub 2015 Feb 2.

Apoptosis is a metazoan process of controlled cell elimination that plays critical roles in embryonic development and adult tissue homeostasis. Apoptosis dysregulation contributes to several important diseases, including cancer. Two distinct yet interconnected signaling pathways control apoptosis by activating a core intracellular machinery of death proteases called caspases. Read More

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February 2015

The apoptotic initiator caspase-8: its functional ubiquity and genetic diversity during animal evolution.

Mol Biol Evol 2014 Dec 8;31(12):3282-301. Epub 2014 Sep 8.

Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

The caspases, a family of cysteine proteases, play multiple roles in apoptosis, inflammation, and cellular differentiation. Caspase-8 (Casp8), which was first identified in humans, functions as an initiator caspase in the apoptotic signaling mediated by cell-surface death receptors. To understand the evolution of function in the Casp8 protein family, casp8 orthologs were identified from a comprehensive range of vertebrates and invertebrates, including sponges and cnidarians, and characterized at both the gene and protein levels. Read More

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December 2014

Induction of mitochondrial dysfunction and oxidative stress in Leishmania donovani by orally active clerodane diterpene.

Antimicrob Agents Chemother 2014 Oct 28;58(10):5916-28. Epub 2014 Jul 28.

Electron Microscopy Unit, Sophisticated Analytical Equipment Facility, CSIR-Central Drug Research Institute, Lucknow, India Academy of Scientific and Innovative Research, New Delhi, India

This study was performed to investigate the mechanistic aspects of cell death induced by a clerodane diterpene (K-09) in Leishmania donovani promastigotes that was previously demonstrated to be safe and orally active against visceral leishmaniasis (VL). K-09 caused depolarization of the mitochondrion and the generation of reactive oxygen species, triggering an apoptotic response in L. donovani promastigotes. Read More

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October 2014

Genomewide survey and characterization of metacaspase gene family in rice (Oryza sativa).

J Genet 2014 Apr;93(1):93-102

National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, People's Republic of China.

Metacaspases (MCs), which are cysteine-dependent proteases found in plants, fungi, and protozoa, may be involved in programmed cell death processes, being distant relatives of metazoan caspases. In this study, we analysed the structures, phylogenetic relationship, genome localizations, expression patterns and domestic selections of eight MC genes identified in rice (OsMC). Alignment analysis of the corresponding protein sequences suggested OsMC proteins can be classified into two subtypes. Read More

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Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats.

Mol Cell Endocrinol 2014 Mar 4;383(1-2):10-20. Epub 2013 Dec 4.

Department of Zoology, University of Delhi, New Delhi 110007, India.

Quercetin, as a flavonoid, has been recognized to possess dual properties of an oxidant and antioxidant as well. The role of quercetin (QC), as an antioxidant in countering estradiol-3-benzoate (EB) induced adverse effects and germ cell apoptosis in adult rat testis was presently investigated. Adult rats received EB (0. Read More

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The Arabidopsis metacaspase9 degradome.

Plant Cell 2013 Aug 20;25(8):2831-47. Epub 2013 Aug 20.

Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.

Metacaspases are distant relatives of the metazoan caspases, found in plants, fungi, and protists. However, in contrast with caspases, information about the physiological substrates of metacaspases is still scarce. By means of N-terminal combined fractional diagonal chromatography, the physiological substrates of metacaspase9 (MC9; AT5G04200) were identified in young seedlings of Arabidopsis thaliana on the proteome-wide level, providing additional insight into MC9 cleavage specificity and revealing a previously unknown preference for acidic residues at the substrate prime site position P1'. Read More

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In-silico analysis of caspase-3 and -7 proteases from blood-parasitic Schistosoma species (Trematoda) and their human host.

Bioinformation 2013 25;9(9):456-63. Epub 2013 May 25.

Bioinformatics Centre, North-Eastern Hill University, Shillong 793022, Meghalaya, India ; Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India.

Proteolytic enzymes of the caspase family, which reside as latent precursors in most nucleated metazoan cells, are core effectors of apoptosis. Of them, the executioner caspases- 3 and -7 exist within the cytosol as inactive dimers and are activated by a process called dimerization. Caspase inhibition is looked upon as a promising approach for treating multiple diseases. Read More

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Unveiling interactions among mitochondria, caspase-like proteases, and the actin cytoskeleton during plant programmed cell death (PCD).

PLoS One 2013 6;8(3):e57110. Epub 2013 Mar 6.

Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.

Aponogeton madagascariensis produces perforations over its leaf surface via programmed cell death (PCD). PCD begins between longitudinal and transverse veins at the center of spaces regarded as areoles, and continues outward, stopping several cells from these veins. The gradient of PCD that exists within a single areole of leaves in an early stage of development was used as a model to investigate cellular dynamics during PCD. Read More

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September 2013

Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma.

Proc Natl Acad Sci U S A 2013 Mar 19;110(10):3943-8. Epub 2013 Feb 19.

Laboratory of Developmental Genetics, The Rockefeller University, New York, NY 10065, USA.

Cell death is a common metazoan cell fate, and its inactivation is central to human malignancy. In Caenorhabditis elegans, apoptotic cell death occurs via the activation of the caspase CED-3 following binding of the EGL-1/BH3-only protein to the antiapoptotic CED-9/BCL2 protein. Here we report a major alternative mechanism for caspase activation in vivo involving the F-box protein DRE-1. Read More

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New types of metacaspases in phytoplankton reveal diverse origins of cell death proteases.

C J Choi J A Berges

Cell Death Dis 2013 Feb 14;4:e490. Epub 2013 Feb 14.

Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA.

Metacaspases are evolutionarily distant homologs of caspases that are found outside the metazoan and are known to have key roles in programmed cell death (PCD). Two types of metacaspases (types I and II) have been defined in plants based on their domain structures; these have similarities to metazoan 'initiator' and 'executioner' caspases. However, we know little about metacaspases in unicellular organisms and even less about their roles in cell death. Read More

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February 2013

Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis.

Cell Death Differ 2012 Dec 24;19(12):2040-8. Epub 2012 Aug 24.

Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158-2330, USA.

Caspases, cysteine proteases with aspartate specificity, are key players in programmed cell death across the metazoan lineage. Hundreds of apoptotic caspase substrates have been identified in human cells. Some have been extensively characterized, revealing key functional nodes for apoptosis signaling and important drug targets in cancer. Read More

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December 2012

Cytoprotective effects of fruit pulp of Eugenia jambolana on H 2 O 2 -induced oxidative stress and apoptosis in rat Leydig cells in vitro.

Andrologia 2013 Jun 26;45(3):145-57. Epub 2012 Jun 26.

Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Delhi- 110067, India.

This study was undertaken to investigate the cytoprotective effect of the fruit pulp of Eugenia jambolana (50-250 μg ml(-1) ) against the damage induced by H 2 O 2 (100 μm) exposure to Leydig cells in vitro. Cell survival with extract was found comparable to similar effects by N-acetyl-l-cysteine. H 2 O 2 -induced rise in thiobarbituric acid reactive substance formation and decline in the activity and expression of antioxidant enzymes like superoxide dismutase, catalase and glutathione-s-transferase were effectively checked. Read More

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