564 results match your criteria metacyclic forms

Characterization and functional analysis of the proteins Prohibitin 1 and 2 in Trypanosoma cruzi.

PLoS Negl Trop Dis 2021 Apr 8;15(4):e0009322. Epub 2021 Apr 8.

Instituto de Biotecnología. Departamento de Parasitología, Grupo CTS183, Universidad de Granada, Spain.

Background: Chagas disease is the third most important neglected tropical disease. There is no vaccine available, and only two drugs are generally prescribed for the treatment, both of which with a wide range of side effects. Our study of T. Read More

View Article and Full-Text PDF

Interaction of Gp82 With Host Cell LAMP2 Induces Protein Kinase C Activation and Promotes Invasion.

Front Cell Infect Microbiol 2021 12;11:627888. Epub 2021 Mar 12.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

The surface molecule gp82 of metacyclic trypomastigote (MT) forms of , the protozoan parasite that causes Chagas disease, mediates the host cell invasion, a process critical for the establishment of infection. Gp82 is known to bind to the target cell in a receptor-dependent manner, triggering Ca signal, actin cytoskeleton rearrangement and lysosome spreading. The host cell receptor for gp82 was recently identified as LAMP2, the major lysosome membrane-associated protein. Read More

View Article and Full-Text PDF

Metacyclogenesis of Trypanosoma cruzi in B. ferroae (Reduviidae: Triatominae) and feces infectivity under laboratory conditions

Biomedica 2021 03 19;41(1):179-186. Epub 2021 Mar 19.

Grupo de Investigaciones en Ciencias Básicas y Aplicadas para la Sostenibilidad, Facultad de Ciencias Exactas, Naturales y Agropecuarias, Universidad de Santander, Bucaramanga, Colombia.

Introduction: Belminus ferroae is a triatominae with entomophagous behavior. However, it may occasionally feed on vertebrates. Currently, there is no evidence of natural infection with Trypanosoma cruzi or the occurrence of metacyclogenesis in this species. Read More

View Article and Full-Text PDF

All Roads Lead to Cytosol: Multi-Strategic Approach to Invasion.

Front Cell Infect Microbiol 2021 5;11:634793. Epub 2021 Mar 5.

CONICET-Universidad de Buenos Aires, IQUIBICEN, Ciudad de Buenos Aires, Argentina.

has a complex life cycle involving four developmental stages namely, epimastigotes, metacyclic trypomastigotes, amastigotes and bloodstream trypomastigotes. Although trypomastigotes are the infective forms, extracellular amastigotes have also shown the ability to invade host cells. Both stages can invade a broad spectrum of host tissues, in fact, almost any nucleated cell can be the target of infection. Read More

View Article and Full-Text PDF

Stage-Specific Differential Gene Expression of Glutathione Peroxidase in and .

Rep Biochem Mol Biol 2020 Oct;9(3):324-330

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: and are the etiological agents of cutaneous leishmaniosis. Leishmania species cause a board spectrum of phenotypes. A small number of genes are differentially expressed between them that have likely an important role in the disease phenotype. Read More

View Article and Full-Text PDF
October 2020

A Nuclear Magnetic Resonance-Based Metabolomic Study to Identify Metabolite Differences between Iranian Isolates of major and .

Iran J Med Sci 2021 Jan;46(1):43-51

Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Cutaneous leishmaniasis caused by species () is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between , we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of and by proton nuclear magnetic resonance (H-NMR).

Methods: and were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. Read More

View Article and Full-Text PDF
January 2021

New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs.

PLoS Negl Trop Dis 2020 12 28;14(12):e0009015. Epub 2020 Dec 28.

Vector Behavior and Pathogen Interaction Group, Instituto René Rachou, Fundação Oswaldo Cruz-FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.

Trypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host's skin during a bloodmeal. Infected mammals behave as parasite reservoirs for several months and despite intensive research, some major aspects of T. Read More

View Article and Full-Text PDF
December 2020

The tissue specific tropism in Trypanosoma cruzi. Is it true?

Acta Trop 2021 Jan 4;213:105736. Epub 2020 Nov 4.

Investigaciones Parasitológicas "J.F. Torrealba", Departamento de Biología, Facultad de Ciencias, Universidad de Los Andes, Mérida, 5101, Venezuela.

Systematic microscopical observations on tissues from mice, inoculated with different Trypanosoma cruzi isolates, were carried out in order to assess whether the parasite expresses tissue-specific tropism, or if it can invade tissues pervasively within the mammal host. A total of ninety mice were included in the study. Sixty, subcutaneously-inoculated with 15 × 10T. Read More

View Article and Full-Text PDF
January 2021

Quantitative proteomic analysis reveals differentially expressed proteins in Leishmania major metacyclogenesis.

Microb Pathog 2020 Dec 2;149:104557. Epub 2020 Oct 2.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Iran. Electronic address:

Leishmaniasis is an infectious disease caused by Leishmania that widespread in 98 countries. The differentiation of Leishmania (L) from procyclic to metacyclic promastigote has occurred along with morphological and biochemical changes in proteome scale. We aim here to identify the proteomes of two successive developmental forms (procyclic and metacyclic promastigotes) from Leishmania major isolates using SWATH-MS quantitative proteomics technique. Read More

View Article and Full-Text PDF
December 2020

Halictine-2 antimicrobial peptide shows promising anti-parasitic activity against Leishmania spp.

Exp Parasitol 2020 Nov 3;218:107987. Epub 2020 Sep 3.

Cell Death and Differentiation Research Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address:

The protozoan parasite Leishmania spp. causes leishmaniases, a group of diseases creating serious health problems in many parts of the world with significant resistance to existing drugs. Insect derived antimicrobial peptides are promising alternatives to conventional drugs against several human disease-causing pathogens because they do not generate resistance. Read More

View Article and Full-Text PDF
November 2020

Ectonucleotidases from trypomastigotes from different sources and various genetic backgrounds of Trypanosoma cruzi potentiate their infectivity and host inflammation.

Cytokine 2020 Dec 28;136:155255. Epub 2020 Aug 28.

Laboratory of Immunobiology of the Inflammation, Department of Biological Sciences, Universidade Federal de Ouro Preto, MG, Brazil. Electronic address:

Distinct populations of Trypanosoma cruzi interact with mammalian cardiac muscle cells causing different inflammation patterns and low heart functionality. During T. cruzi infection, the extracellular ATP is hydrolyzed to tri- and/or diphosphate nucleotides, based on the infectivity, virulence, and regulation of the inflammatory response. Read More

View Article and Full-Text PDF
December 2020

Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei.

Nucleic Acids Res 2020 09;48(15):8704-8723

Department of Microbiology and Immunology, University at Buffalo - Jacobs School of Medicine and Biomedical Sciences, Buffalo, NY 14203, USA.

Trypanosoma brucei is a parasitic protozoan that undergoes a complex life cycle involving insect and mammalian hosts that present dramatically different nutritional environments. Mitochondrial metabolism and gene expression are highly regulated to accommodate these environmental changes, including regulation of mRNAs that require extensive uridine insertion/deletion (U-indel) editing for their maturation. Here, we use high throughput sequencing and a method for promoting life cycle changes in vitro to assess the mechanisms and timing of developmentally regulated edited mRNA expression. Read More

View Article and Full-Text PDF
September 2020

Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications: Study of changes in epigenetic marks through the parasite's metacyclogenesis and life cycle.

J Proteomics 2020 08 29;225:103847. Epub 2020 May 29.

Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil. Electronic address:

Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based (MS-based) data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite's histone sequences by modifying the software EpiProfile 2. Read More

View Article and Full-Text PDF

Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes.

Parasit Vectors 2020 May 14;13(1):255. Epub 2020 May 14.

Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.

Background: Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease. This parasite has high genetic variability and can be divided into six discrete typing units (DTUs). Read More

View Article and Full-Text PDF

Cytokine Gene Expression Alterations in Human Macrophages Infected by .

Cell J 2021 Jan 22;22(4):476-481. Epub 2020 Apr 22.

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic Address:

Objective: Leishmaniasis is caused by members of the species and constitute a group of infective diseases that range from cutaneous lesions to lethal visceral forms. In infected persons, macrophages recognize and eliminate the parasites via phagocytosis. In order to change a hostile environment into an environment adequate for survival and reproduction, the engulfed Leishmania species needs to modulate the function of its host macrophage. Read More

View Article and Full-Text PDF
January 2021

Comparison of gene expression of pyruvate kinase and tryparedoxin peroxidase in metacyclic promastigote forms of Leishmania (L.) tropica and L. major by real-time PCR

Ann Parasitol 2020 ;66(1):13-18

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Two predominant forms of cutaneous leishmaniosis are anthroponotic CL (ACL) and zoonotic CL (ZCL) caused by Leishmania (L.) tropica and L. major in Iran and many countries, respectively. Read More

View Article and Full-Text PDF

The Influence of Environmental Cues on the Development of in Triatominae Vector.

Front Cell Infect Microbiol 2020 21;10:27. Epub 2020 Feb 21.

Laboratório de Bioquímica de Tryps (LaBTryps), Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.

, a hemoflagellate parasite, is the etiological agent of Chagas disease that affects about 6-7 million people worldwide, mostly in Latin America. The parasite life cycle is complex and alternates between an invertebrate host-Triatominae vector-and a mammalian host. The parasite adaptation to the several microenvironments through which it transits is critical to success in establishing infection. Read More

View Article and Full-Text PDF
February 2020

Distinct gene expression patterns in vector-residing Leishmania infantum identify parasite stage-enriched markers.

PLoS Negl Trop Dis 2020 03 3;14(3):e0008014. Epub 2020 Mar 3.

Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America.

Background: Leishmaniasis is a vector-borne neglected disease. Inside the natural sand fly vector, the promastigote forms of Leishmania undergo a series of extracellular developmental stages to reach the infectious stage, the metacyclic promastigote. There is limited information regarding the expression profile of L. Read More

View Article and Full-Text PDF

Endocytosis and Exocytosis in Are Modulated by Bromoenol Lactone.

Front Cell Infect Microbiol 2020 7;10:39. Epub 2020 Feb 7.

Centro de Ciências da Saúde, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.

In the protozoan pathogen , endocytosis, and exocytosis occur mainly in the small area of the flagellar pocket membrane, which makes this parasite an interesting model of strikingly polarized internalization and secretion. Moreover, little is known about vesicle recognition and fusion mechanisms, which are essential for both endo/exocytosis in this parasite. In other cell types, vesicle fusion events require the activity of phospholipase A (PLA), including Ca-independent iPLA and soluble, Ca-dependent sPLA. Read More

View Article and Full-Text PDF
February 2020

Nuclear export of replication protein A in the nonreplicative infective forms of Trypanosoma cruzi.

FEBS Lett 2020 05 2;594(10):1596-1607. Epub 2020 Mar 2.

Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, Brazil.

Replication protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Read More

View Article and Full-Text PDF

Single-cell RNA sequencing of from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens.

Proc Natl Acad Sci U S A 2020 02 21;117(5):2613-2621. Epub 2020 Jan 21.

Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, CT 06520;

Tsetse-transmitted African trypanosomes must develop into mammalian-infectious metacyclic cells in the fly's salivary glands (SGs) before transmission to a new host. The molecular mechanisms that underlie this developmental process, known as metacyclogenesis, are poorly understood. Blocking the few metacyclic parasites deposited in saliva from further development in the mammal could prevent disease. Read More

View Article and Full-Text PDF
February 2020

Transmission potential of paromomycin-resistant Leishmania infantum and Leishmania donovani.

J Antimicrob Chemother 2020 04;75(4):951-957

Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium.

Objectives: Former studies demonstrated quick selection of paromomycin resistance for Leishmania infantum and Leishmania donovani accompanied by increased fitness. The present study aimed to interpret these findings in an epidemiological context by comparing infection of WT and experimentally derived paromomycin-resistant strains in the sand fly vector.

Methods: Depending on the Leishmania species, Lutzomyia longipalpis and Phlebotomus perniciosus or Phlebotomus argentipes sand flies were artificially infected with procyclic promastigotes of WT and paromomycin-resistant L. Read More

View Article and Full-Text PDF

Engineering a single-chain antibody against Trypanosoma cruzi metacyclic trypomastigotes to block cell invasion.

PLoS One 2019 16;14(10):e0223773. Epub 2019 Oct 16.

Departamento de Bioquímica e Biologia Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná,Curitiba, Brasil.

Trypanosoma cruzi is a flagellate protozoan pathogen that causes Chagas disease. Currently there is no preventive treatment and the efficiency of the two drugs available is limited to the acute phase. Therefore, there is an unmet need for innovative tools to block transmission in endemic areas. Read More

View Article and Full-Text PDF

Ascorbate-Dependent Peroxidase (APX) from Leishmania amazonensis Is a Reactive Oxygen Species-Induced Essential Enzyme That Regulates Virulence.

Infect Immun 2019 12 18;87(12). Epub 2019 Nov 18.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA

The molecular mechanisms underlying biological differences between two species that cause cutaneous disease, and , are poorly understood. In , reactive oxygen species (ROS) signaling drives differentiation of nonvirulent promastigotes into forms capable of infecting host macrophages. Tight spatial and temporal regulation of HO is key to this signaling mechanism, suggesting a role for ascorbate-dependent peroxidase (APX), which degrades mitochondrial HO Earlier studies showed that -null parasites are viable, accumulate higher levels of HO, generate a greater yield of infective metacyclic promastigotes, and have increased virulence. Read More

View Article and Full-Text PDF
December 2019

Leishmania amazonensis ferric iron reductase (LFR1) is a bifunctional enzyme: Unveiling a NADPH oxidase activity.

Free Radic Biol Med 2019 11 22;143:341-353. Epub 2019 Aug 22.

Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro (UFRJ), CCS, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil; Institute of National Science and Technology of Structural Biology and Bioimage (INCTBEB), CCS, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, RJ, Brazil. Electronic address:

Leishmania amazonensis is one of leishmaniasis' causative agents, a disease that has no cure and leads to the appearance of cutaneous lesions. Recently, our group showed that heme activates a Na/K ATPase in these parasites through a signaling cascade involving hydrogen peroxide (HO) generation. Heme has a pro-oxidant activity and signaling capacity, but the mechanism by which this molecule increases HO levels in L. Read More

View Article and Full-Text PDF
November 2019

Depletion of Host Cell Focal Adhesion Kinase Increases the Susceptibility to Invasion by Metacyclic Forms.

Front Cell Infect Microbiol 2019 26;9:231. Epub 2019 Jun 26.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase (PTK), is implicated in diverse cellular processes, including the regulation of F-actin dynamics. Host cell F-actin rearrangement is critical for invasion of , the protozoan parasite that causes Chagas disease. It is unknown whether FAK is involved in the internalization process of metacyclic trypomastigote (MT), the parasite form that is important for vectorial transmission. Read More

View Article and Full-Text PDF
February 2020

Further in vivo evidence implying DNA apurinic/apyrimidinic endonuclease activity in Trypanosoma cruzi oxidative stress survival.

J Cell Biochem 2019 10 17;120(10):16733-16740. Epub 2019 May 17.

Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.

Trypanosoma cruzi is under the attack of reactive species produced by its mammalian and insect hosts. To survive, it must repair its damaged DNA. We have shown that a base excision DNA repair (BER)-specific parasite TcAP1 endonuclease is involved in the resistance to H O . Read More

View Article and Full-Text PDF
October 2019

Functional genomics in sand fly-derived Leishmania promastigotes.

PLoS Negl Trop Dis 2019 05 9;13(5):e0007288. Epub 2019 May 9.

Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas), Madrid, Spain.

Background: Leishmania development in the sand fly gut leads to highly infective forms called metacyclic promastigotes. This process can be routinely mimicked in culture. Gene expression-profiling studies by transcriptome analysis have been performed with the aim of studying promastigote forms in the sand fly gut, as well as differences between sand fly-and culture-derived promastigotes. Read More

View Article and Full-Text PDF

Laboratory transmission of an Asian strain of Leishmania tropica by the bite of the southern European sand fly Phlebotomus perniciosus.

Int J Parasitol 2019 05 30;49(6):417-421. Epub 2019 Mar 30.

Unit of Vector-borne Diseases, Department of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Electronic address:

Imported cases of anthroponotic cutaneous leishmaniasis due to Leishmania tropica are increasingly documented in Europe. We investigated the ability of Phlebotomus perniciosus, a competent vector of Leishmania infantum widespread in southwestern Europe, to support the growth and transmissibility of an Asian strain of L. tropica recently isolated from a refugee. Read More

View Article and Full-Text PDF

Nutritional stress targets LeishIF4E-3 to storage granules that contain RNA and ribosome components in Leishmania.

PLoS Negl Trop Dis 2019 03 14;13(3):e0007237. Epub 2019 Mar 14.

Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Leishmania parasites lack pathways for de novo purine biosynthesis. The depletion of purines induces differentiation into virulent metacyclic forms. In vitro, the parasites can survive prolonged periods of purine withdrawal changing their morphology to long and slender cells with an extended flagellum, and decreasing their translation rates. Read More

View Article and Full-Text PDF