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CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli.

J Hazard Mater 2022 Jan 6;428:128225. Epub 2022 Jan 6.

College of Plant Protection, Hunan Agricultural University, Changsha 410128, China. Electronic address:

Long-term and excessive herbicide use has led to some environmental concerns and especially, herbicide resistance evolution in weeds. Here, we confirmed acetolactate synthase (ALS) inhibiting herbicide penoxsulam resistance and cross resistance to acetyl-coenzyme carboxylase (ACCase) inhibiting herbicides (cyhalofop-butyl and metamifop) in a global weed Echinochloa crus-galli population resistant to these herbicides (R). Penoxsulam metabolism study indicated that degradation rate was significantly higher in R than susceptible E. Read More

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January 2022

HRPIF data mining based on data-dependent/independent acquisition for Rhei Radix et Rhizoma metabolite screening in rats.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 Jan 10;1190:123095. Epub 2022 Jan 10.

Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cell Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China. Electronic address:

In traditional Chinese medicine (TCM), components with identical nuclei often share structural similarity, indicating the possibility of similar second-level mass spectrometry (MS/MS) fragments. High-resolution product-ion filter (HRPIF) technique can be utilized to identify metabolites, with similar fragments, in vivo. In principle, this technique applies to TCM; however, its application has been restricted due to the limitations of traditional MS/MS data acquisition. Read More

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January 2022

Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 Jan 6;1190:123100. Epub 2022 Jan 6.

Drug Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsuruta-machi, Utsunomiya, Tochigi 320-0851, Japan.

Nicotine is classified as a stimulant, and its use is banned in horse racing and equestrian sports by the International Federation of Horseracing Authorities and the Fédération Équestre Internationale, respectively. Because nicotine is a major alkaloid of tobacco leaves, there is a potential risk that doping control samples may be contaminated by tobacco cigarettes or smoke during sample collection. In order to differentiate the genuine doping and sample contamination with tobacco leaves, it is necessary to monitor unique metabolites as biomarkers for nicotine administration and intake. Read More

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January 2022

Metabolomics analysis of urine from rats given long-term high-protein diet using ultra-high-performance liquid chromatography-mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Dec 30;1190:123082. Epub 2021 Dec 30.

National Key Discipline Laboratory, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, PR China. Electronic address:

Previous studies have indicated high-protein diet (HPD) promotes weight loss and improves metabolic parameters, but most of these studies have focused on the impact of short-term, long-term effects remain unclear. In this study, male Wistar rats were fed two diets for 88 weeks: normal control diet (NCD, 20.5% of energy as protein) or HPD (30. Read More

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December 2021

Study on the stability and oral bioavailability of curcumin loaded (-)-epigallocatechin-3-gallate/poly(N-vinylpyrrolidone) nanoparticles based on hydrogen bonding-driven self-assembly.

Food Chem 2022 Jan 8;378:132091. Epub 2022 Jan 8.

College of Food Science, Southwest University, Chongqing 400715, People's Republic of China; Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, People's Republic of China. Electronic address:

The biological activity and absorption of curcumin (Cur) is limited in application due to its low water solubility, poorstabilityand rapid metabolism. In this work, Cur loaded (-)-epigallocatechin-3-gallate (EGCG)/poly(N-vinylpyrrolidone) (PVP) nanoparticles (CEP-NPs) was successfully fabricated via self-assembly driven by hydrogen bonding, providing with desirable Cur-loading efficiency, high stability, strong antioxidant capacity, and pH-triggered intestinal targeted release properties. Molecular dynamics simulations further indicated the Cur was coated with EGCG and PVP in CEP-NPs and high acid prolonged release property was attribute to low ionization degree of EGCG. Read More

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January 2022

Mycoplasma synoviae dihydrolipoamide dehydrogenase is an immunogenic fibronectin/plasminogen binding protein and a putative adhesin.

Vet Microbiol 2021 Dec 31;265:109328. Epub 2021 Dec 31.

Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, PR China. Electronic address:

Mycoplasma synoviae (M. synoviae) is an important avian pathogen that causes arthritis and airsacculitis in young chickens and turkeys. Infection by M. Read More

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December 2021

The impact of motion artifacts on quantitative optical coherence tomography angiography analysis in Parkinson's disease.

Parkinsonism Relat Disord 2022 Jan 10;95:57-58. Epub 2022 Jan 10.

Department of Neurology, University of Lübeck, Germany; Institute of Neurogenetics, University of Lübeck, Germany; Center for Brain, Behavior and Metabolism (CBBM), University of Lübeck, Germany.

We have previously shown that OCTA imaging in PD patients can be challenging. Our data suggest that retinal perfusion is reduced in both plexuses in PD, which may serve as a noninvasive biomarker in the future. Yet, control of motion artifacts in OCTA measurements is critical in this motor-impaired cohort. Read More

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January 2022

Integrated hepatic single-cell RNA sequencing and untargeted metabolomics reveals the immune and metabolic modulation of Qing-Fei-Pai-Du decoction in mice with coronavirus-induced pneumonia.

Phytomedicine 2022 Jan 2;97:153922. Epub 2022 Jan 2.

School of Pharmacy, Second Military Medical University, Shanghai 200433, China; The Research Center for Traditional Chinese Medicine, Shanghai Institute of Infectious Diseases and Biosafety, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Background: Although Qing-Fei-Pai-Du decoction (QFPDD) is extensively used clinically to treat COVID-19 patients, the mechanism by which it modulates the immunological and metabolic functions of liver tissue remains unknown.

Purpose: The purpose of this study is to investigate the mechanism of action of QFPDD in the treatment of mice with coronavirus-induced pneumonia by combining integrated hepatic single-cell RNA sequencing and untargeted metabolomics.

Methods: We developed a human coronavirus pneumonia model in BALB/c mice by infecting them with human coronavirus HCoV-229E with stimulating them with cold-damp environment. Read More

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January 2022

Untargeted GC-MS metabolomics reveals the metabolic responses in the gills of Chinese mitten crab (Eriocheir sinensis) subjected to air-exposure stress.

Ecotoxicol Environ Saf 2022 Jan 12;231:113159. Epub 2022 Jan 12.

Department of Marine Technology, School of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng 224051, China.

Desiccation is a common stress experienced by crabs during aquaculture and transportation. In China, the crustacean, Chinese mitten crab (Eriocheir sinensis), is economically important. However, little is known about the molecular pathways underlying physiological stress. Read More

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January 2022

Relaxed control of sugar utilization in Parageobacillus thermoglucosidasius DSM 2542.

Microbiol Res 2021 Dec 30;256:126957. Epub 2021 Dec 30.

Department of Biology and Biochemistry, University of Bath, UK.

Though carbon catabolite repression (CCR) has been intensively studied in some more characterised organisms, there is a lack of information of CCR in thermophiles. In this work, CCR in the thermophile, Parageobacillus thermoglucosidasius DSM 2542 has been studied during growth on pentose sugars in the presence of glucose. Physiological studies under fermentative conditions revealed a loosely controlled CCR when DSM 2542 was grown in minimal medium supplemented with a mixture of glucose and xylose. Read More

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December 2021

Single-cell Transcriptomic Profiling of the Hypothalamic Median Eminence during Aging.

J Genet Genomics 2022 Jan 12. Epub 2022 Jan 12.

State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100101, China; Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Beijing 100101, China; Chinese Institute for Brain Research, Beijing 102206, China; Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address:

Aging is a slow and progressive natural process that compromises the normal functions of cells, tissues, organs and systems. The aging of the hypothalamic median eminence (ME), a structural gate linking neural and endocrine systems, may impair hormone release, energy homeostasis and central sensing of circulating molecules, leading to systemic and reproductive aging. However, the molecular and cellular features of ME aging remain largely unknown. Read More

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January 2022

Influence of pH on physiological and behavioral responses of Pomacea canaliculata.

Comp Biochem Physiol A Mol Integr Physiol 2022 Jan 12:111153. Epub 2022 Jan 12.

Graduate Program in Pharmacology, Federal University of Santa Maria, 97105-900 Santa Maria, RS, Brazil; Department of Physiology and Pharmacology, Federal University of Santa Maria, 97105-900 Santa Maria, RS, Brazil. Electronic address:

The physiological and behavioral responses of Pomacea canaliculata exposed to different pH values (4, 5, 6, 7, 8, 9, and 10) were evaluated. Survival, behavior (avoidance), metabolites (mantle), net ion fluxes (Na and K), and ATPase activity (gills) were the parameters analyzed. The final survival rates were 100% (pH 4-9) and 90% (pH 10), and the groups did not differ significantly. Read More

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January 2022

Tanshinone IIA and its derivative activate thermogenesis in adipocytes and induce "beiging" of white adipose tissue.

Mol Cell Endocrinol 2022 Jan 12:111557. Epub 2022 Jan 12.

Department of Biochemistry, Molecular Cancer Research Center, School of Medicine, Sun yat-sen University, Shenzhen, 518107, Guangdong Province, China. Electronic address:

Tanshinone IIA (TAN2A) is a major active ingredient of Salvia miltiorrhiza used in traditional Chinese medicine and tanshinone 20 (TAN20) is a derivative of TAN2A. In this study, we examined the effects of TAN2A and TAN20 on adipogenesis, lipid metabolism, and thermogenesis. Our experiments showed that both TAN2A and TAN20 increased mitochondria content in adipose tissue, enhanced energy expenditure, reduced body weight, and improved insulin sensitivity and metabolic homeostasis in obese and diabetic mouse models. Read More

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January 2022

Therapeutic effect of Patrinia villosa on TNBS-induced ulcerative colitis via metabolism, vitamin D receptor and NF-κB signaling pathways.

J Ethnopharmacol 2022 Jan 12:114989. Epub 2022 Jan 12.

School of Pharmaceutical Science, Liaoning University, No.66 Chongshan Middle Road, Shenyang, 110036, PR China. Electronic address:

Ethnopharmacological Relevance: Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon. Patrinia villosa Juss. (P. Read More

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January 2022

Hypoglycemic and hypolipidemic effects of Epigynum auritum in high fat diet and streptozotocin-induced diabetic rats.

J Ethnopharmacol 2022 Jan 12:114986. Epub 2022 Jan 12.

Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China. Electronic address:

Ethnopharmacological Relevance: Epigynum auritum is mainly distributed in Southwest China, and has been used as a "dai" folk medicine with promising Besides, the leaves and barks of E. auritum have detoxifying, analgesic and relieving swelling effects. Previous studies evidenced that E. Read More

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January 2022

Thermal and wine processing enhanced Clematidis Radix et Rhizoma ameliorate collagen Ⅱ induced rheumatoid arthritis in rats.

J Ethnopharmacol 2022 Jan 12:114993. Epub 2022 Jan 12.

State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, China Pharmaceutical University, Nanjing, 210009, PR China; School of Pharmacy, Xinjiang Medical University, Urumqi, 830011, PR China. Electronic address:

Ethnopharmacological Relevance: Clematidis Radix et Rhizoma, a kind of traditional Chinese medicine, is derived from Clematis chinensis Osbeck, Clematis hexapetala Pall. and Clematis manshurica Rupr. This herb shows great effects on expelling wind and dispelling dampness in ancient and it has anti-inflammatory and analgesic activity in modern clinical application. Read More

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January 2022

Mild calorie restriction, but not 17α-estradiol, extends ovarian reserve and fertility in female mice.

Exp Gerontol 2022 Jan 12:111669. Epub 2022 Jan 12.

Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Electronic address:

Calorie restriction (CR) (25-40%) is the most commonly studied strategy for curtailing age-related disease and has also been found to extend reproductive lifespan in female mice. However, the effects of mild CR (10%), which is sustainable, on ovarian aging has not yet been addressed. 17α-estradiol (17α-E2) is another intervention shown to positively modulate healthspan and lifespan in mice but its effects on female reproduction remain unclear. Read More

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January 2022

Transcriptomic changes in the hypothalamus of ovariectomized mice: Data from RNA-seq analysis.

Ann Anat 2022 Jan 12:151886. Epub 2022 Jan 12.

Department of Anatomy and Histoembryology, School of Basic Medical Science, Peking University, Beijing 100191, China. Electronic address:

Background: Menopausal symptoms can affect the physical and mental health of females and are often related to abnormal function of the hypothalamus. In this study, we evaluated changes in the hypothalamus transcriptome in ovariectomized mice to identify key mRNAs, and systematically elucidated the possible molecular mechanisms underlying the menopausal syndrome to provide a theoretical basis for clinical diagnosis and treatment.

Methods: Forty-six adult female C57BL/6J mice were randomly divided into SHAM and OVX groups, 23 mice per group. Read More

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January 2022

Factors Associated with Progression of Different Prediabetic Status to Diabetes: A Community-based Cohort Study.

Diabetes Res Clin Pract 2022 Jan 12:109193. Epub 2022 Jan 12.

Department of Endocrinology and Metabolism, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China. Electronic address:

Aims: To examine the predictive factors associated with the progression of different prediabetic status to diabetes.

Methods: A two-year retrospective cohort study was conducted on 5741 participants aged 40 years or older. Finally, 1685 participants with prediabetes defined by IFG (impaired fasting glucose), IGT (impaired glucose tolerance) and CGI (combined IFG and IGT) were included. Read More

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January 2022

Expanded substrate specificity supported by P1' and P2' residues enables bacterial dipeptidyl-peptidase 7 to degrade bioactive peptides.

J Biol Chem 2022 Jan 12:101585. Epub 2022 Jan 12.

Department of Oral Molecular Biology, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan. Electronic address:

Dipeptide production from extracellular proteins is crucial for Porphyromonas gingivalis, a pathogen related to chronic periodontitis, because its energy production is entirely dependent on the metabolism of amino acids predominantly incorporated as dipeptides. These dipeptides are produced by periplasmic dipeptidyl-peptidase (DPP)4, DPP5, DPP7, and DPP11. Although the substrate specificities of these four DPPs cover most amino acids at the penultimate position from the N-terminus (P1), no DPP is known to cleave penultimate Gly, Ser, Thr, or His. Read More

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January 2022

Associations of variability in body weight and glucose levels with the risk of hip fracture in people with diabetes.

Metabolism 2022 Jan 12:155135. Epub 2022 Jan 12.

Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea; Department of Medical Informatics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea. Electronic address:

Background: Diabetes is associated with a high risk of fragility fracture. However, there are controversies regarding the effect of fluctuations in metabolic parameters on the risk of fracture. We aimed to investigate the associations of body weight or glucose variability or their combination with the risk of hip fracture in people with diabetes. Read More

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January 2022

The effect of dietary patterns on non-alcoholic fatty liver disease diagnosed by biopsy or magnetic resonance in adults: a systematic review of randomised controlled trials.

Metabolism 2022 Jan 12:155136. Epub 2022 Jan 12.

Department of Medicine, Division of Endocrinology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA; Department of Endocrinology, Boston VA Healthcare System, Boston, MA, USA.

Adhering to specific dietary patterns might hold promise as a lifestyle modification treatment of non-alcoholic fatty liver disease (NAFLD). The aim of this systematic review was to examine the effect of dietary patterns on changes in hepatic fat content, liver enzymes and metabolic syndrome components. We searched Pubmed, Embase, CINAHL and Web of Science for randomised controlled trials published in English until April 2020, comparing a specific dietary pattern with no treatment, usual care, or a different diet in adults with NAFLD. Read More

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January 2022

Gestational insulin resistance is mediated by the gut microbiome-indoleamine 2,3-dioxygenase axis.

Gastroenterology 2022 Jan 12. Epub 2022 Jan 12.

Division of Endocrinology, Diabetes, and Metabolism and UIC, Chicago-IL, U.S.A.; Jesse Brown Veterans Affair Medical Center, Chicago-IL, U.S.A.. Electronic address:

Background And Aims: Normal gestation involves reprogramming of maternal gut microbiome (GM) that contributes to maternal metabolic changes by unclear mechanisms. This study aimed to understand the mechanistic underpinnings of GM - maternal metabolism interaction.

Methods: The GM and plasma metabolome of CD1, NIH-Swiss and C57 mice were analyzed using 16S rRNA sequencing and untargeted LC-MS throughout gestation. Read More

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January 2022

Hyperammonemia-induced changes in the cerebral transcriptome and proteome.

Anal Biochem 2022 Jan 12:114548. Epub 2022 Jan 12.

Clinic for Gastroenterology, Hepatology and Infectious Diseases, Germany. Electronic address:

Molecular alterations underlying cerebral impairment in hyperammonemic disorders such as in hepatic encephalopathy (HE) are only poorly understood. Using transcriptomics and proteomics on brains of mice with systemic hyperammonemia resulting from knockout of hepatic glutamine synthetase (LGS-KO) we identified up to 214 genes and 34 proteins whose expressions were altered in brains of LGS-KO mice in a brain region-specific way. Differentially expressed genes were enriched for those related to oxidative stress, cell proliferation, heme metabolism and others. Read More

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January 2022

Complement activation induces excessive T cell cytotoxicity in severe COVID-19.

Cell 2021 Dec 28. Epub 2021 Dec 28.

Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Center for Infection Research (HZI), Würzburg, Germany.

Severe COVID-19 is linked to both dysfunctional immune response and unrestrained immunopathology, and it remains unclear whether T cells contribute to disease pathology. Here, we combined single-cell transcriptomics and single-cell proteomics with mechanistic studies to assess pathogenic T cell functions and inducing signals. We identified highly activated CD16 T cells with increased cytotoxic functions in severe COVID-19. Read More

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December 2021

Adaptive capacity to dietary Vitamin B12 levels is maintained by a gene-diet interaction that ensures optimal life span.

Aging Cell 2022 Jan 8;21(1):e13518. Epub 2021 Dec 8.

Molecular Aging Laboratory, National Institute of Immunology, New Delhi, India.

Diet regulates complex life-history traits such as longevity. For optimal lifespan, organisms employ intricate adaptive mechanisms whose molecular underpinnings are less known. We show that Caenorhabditis elegans FLR-4 kinase prevents lifespan differentials on the bacterial diet having higher Vitamin B12 levels. Read More

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January 2022

Eukaryotic elongation factor 2 kinase regulates foam cell formation via translation of CD36.

FASEB J 2022 Feb;36(2):e22154

Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.

Eukaryotic elongation factor 2 kinase (eEF2K) is an atypical protein kinase that controls protein synthesis in cells under stress. Although well studied in cancer, less is known about its roles in chronic inflammatory diseases. Here, we examined its regulation of macrophage cholesterol handling in the context of atherosclerosis. Read More

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February 2022

Time-restricted feeding during the inactive phase abolishes the daily rhythm in mitochondrial respiration in rat skeletal muscle.

FASEB J 2022 Feb;36(2):e22133

Laboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology, Endocrinology, and Metabolism, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Shift-workers show an increased incidence of type 2 diabetes mellitus (T2DM). A possible mechanism is the disruption of the circadian timing of glucose homeostasis. Skeletal muscle mitochondrial function is modulated by the molecular clock. Read More

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February 2022

The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.

FASEB J 2022 Feb;36(2):e22142

Centre for Biomedical Technologies, Queensland University of Technology, Brisbane, Queensland, Australia.

The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone. The hallmark of CCZ characteristics in osteoarthritis (OA) is less well known. The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity. Read More

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February 2022

Paired related homeobox 1 attenuates autophagy via acetyl-CoA carboxylase 1-regulated fatty acid metabolism in salivary adenoid cystic carcinoma.

FEBS Open Bio 2022 Jan 15. Epub 2022 Jan 15.

Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao, 266003, China.

Autophagy can affect the invasion and metastasis of carcinoma. Our previous study has shown that invasion and epithelial-mesenchymal transition in salivary adenoid cystic carcinoma (SACC) can be promoted by the metabolic reprogramming of free fatty acids (FFAs). However, the effect of FFA metabolism on autophagy in SACC remains unknown. Read More

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January 2022