338 results match your criteria meshworks heterogeneous


Parasite-Mediated Remodeling of the Host Microfilament Cytoskeleton Enables Rapid Egress of Trypanosoma cruzi following Membrane Rupture.

mBio 2021 06 22;12(3):e0098821. Epub 2021 Jun 22.

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Chagas' disease arises as a direct consequence of the lytic cycle of Trypanosoma cruzi in the mammalian host. While invasion is well studied for this pathogen, study of egress has been largely neglected. Here, we provide the first description of T. Read More

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Structural insight into transmissive mutant huntingtin species by correlative light and electron microscopy and cryo-electron tomography.

Biochem Biophys Res Commun 2021 Jun 10;560:99-104. Epub 2021 May 10.

Department of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA; Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA. Electronic address:

Aggregates of mutant huntingtin (mHTT) containing an expanded polyglutamine (polyQ) tract are hallmarks of Huntington's Disease (HD). Studies have shown that mHTT can spread between cells, leading to the propagation of misfolded protein pathology. However, the structure of transmissive mHTT species, and the molecular mechanisms underlying their transmission remain unknown. Read More

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Membrane binding controls ordered self-assembly of animal septins.

Elife 2021 Apr 13;10. Epub 2021 Apr 13.

AMOLF, Department of Living Matter, Biological Soft Matter group, Amsterdam, Netherlands.

Septins are conserved cytoskeletal proteins that regulate cell cortex mechanics. The mechanisms of their interactions with the plasma membrane remain poorly understood. Here, we show by cell-free reconstitution that binding to flat lipid membranes requires electrostatic interactions of septins with anionic lipids and promotes the ordered self-assembly of fly septins into filamentous meshworks. Read More

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Actin bundle architecture and mechanics regulate myosin II force generation.

Biophys J 2021 05 31;120(10):1957-1970. Epub 2021 Mar 31.

James Franck Institute, University of Chicago, Chicago, Illinois; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois; Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois; Department of Physics, University of Chicago, Chicago, Illinois. Electronic address:

The actin cytoskeleton is a soft, structural material that underlies biological processes such as cell division, motility, and cargo transport. The cross-linked actin filaments self-organize into a myriad of architectures, from disordered meshworks to ordered bundles, which are hypothesized to control the actomyosin force generation that regulates cell migration, shape, and adhesion. Here, we use fluorescence microscopy and simulations to investigate how actin bundle architectures with varying polarity, spacing, and rigidity impact myosin II dynamics and force generation. Read More

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Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins.

J Cell Biol 2021 Apr;220(4)

Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using datasets prepared from subpixel and segmentation analyses of 3D-structured illumination microscopy images of WT and lamin isoform knockout mouse embryo fibroblasts, we determined with high precision the spatial association of NPCs with specific lamin isoform fibers. These relationships are retained in the enlarged lamin meshworks of Lmna-/- and Lmnb1-/- fibroblast nuclei. Read More

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Molecular Tuning of Filamin A Activities in the Context of Adhesion and Migration.

Front Cell Dev Biol 2020 20;8:591323. Epub 2020 Nov 20.

Centre de Physiopathologie de Toulouse Purpan, INSERM, CNRS, Université de Toulouse, UPS, Toulouse, France.

The dynamic organization of actin cytoskeleton meshworks relies on multiple actin-binding proteins endowed with distinct actin-remodeling activities. Filamin A is a large multi-domain scaffolding protein that cross-links actin filaments with orthogonal orientation in response to various stimuli. As such it plays key roles in the modulation of cell shape, cell motility, and differentiation throughout development and adult life. Read More

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November 2020

Multiscalar Temporality in Human Behaviour: A Case Study of Constraint Interdependence in Psychotherapy.

Front Psychol 2020 20;11:1685. Epub 2020 Aug 20.

Centre for Human Interactivity, Department of Language and Communication, University of Southern Denmark, Odense, Denmark.

Ecological psychology (EP) and the enactive approach (EA) may benefit from a more focused view of lived temporality and the underlying temporal multiscalar nature of human living. We propose multiscalar temporality (MT) as a framework that complements EP and EA, and moves beyond their current conceptualisation of timescales and inter-scale relationships in organism-environment dynamical systems. MT brings into focus the wide ranging and meshwork-like interdependencies at play in human living and the questions concerning how agents are intimately entangled in such meshworks, utilising them as resources for skilful living. Read More

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Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration.

World J Gastroenterol 2020 Jul;26(27):3899-3916

Faculty of Medicine, Ivane Javakhishvili Tbilisi State University, Tbilisi 0159, Georgia.

Background: The phenomenon of liver regeneration after partial hepatectomy (PH) is still a subject of considerable interest due to the increasing frequency of half liver transplantation on the one hand, and on the other hand, new surgical approaches which allow removal of massive space-occupying hepatic tumors, which earlier was considered as inoperable. Interestingly, the mechanisms of liver regeneration are extensively studied after PH but less attention is paid to the architectonics of the regenerated organ. Because of this, the question "How does the structure of regenerated liver differ from normal, regular liver?" has not been fully answered yet. Read More

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Sea star-inspired recombinant adhesive proteins self-assemble and adsorb on surfaces in aqueous environments to form cytocompatible coatings.

Acta Biomater 2020 08 2;112:62-74. Epub 2020 Jun 2.

Laboratory of Cell Biology, Research Institute for Biosciences, University of Mons, Place du Parc 23, 7000 Mons, Belgium. Electronic address:

Sea stars adhere to various underwater substrata using an efficient protein-based adhesive secretion. The protein Sfp1 is a major component of this secretion. In the natural glue, it is cleaved into four subunits (Sfp1 Alpha, Beta, Delta and Gamma) displaying specific domains which mediate protein-protein or protein-carbohydrate interactions. Read More

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Cell-type-specific mechanical response and myosin dynamics during retinal lens development in .

Mol Biol Cell 2020 06 22;31(13):1355-1369. Epub 2020 Apr 22.

MRC Laboratory for Molecular Cell Biology.

During organogenesis, different cell types need to work together to generate functional multicellular structures. To study this process, we made use of the genetically tractable fly retina, with a focus on the mechanisms that coordinate morphogenesis between the different epithelial cell types that make up the optical lens. Our work shows that these epithelial cells present contractile apical-medial MyosinII meshworks, which control the apical area and junctional geometry of these cells during lens development. Read More

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Multiple Morphometric Assessment of Microglial Cells in Deafferented Spinal Trigeminal Nucleus.

Front Neuroanat 2019 22;13:103. Epub 2020 Jan 22.

Department of Anatomy, Histology and Neuroscience, Medical School, Autonomous University of Madrid, Madrid, Spain.

Microglia (MG) are the first cells to react to the abnormal incoming signals that follow an injury of sensory nerves and play a critical role in the development and maintenance of neuropathic pain, a common sequel of nerve injuries. Here we present population data on cell number, soma size, and length of processes of MG in the caudal division of the spinal trigeminal nucleus (Sp5C) in control mice and at the peak of microgliosis (7 days) following unilateral transection of the infraorbital nerve (IoN). The study is performed combining several bias- and assumption-free imaging and stereological approaches with different immunolabeling procedures, with the objective of tackling some hard problems that often hinder proper execution of MG morphometric studies. Read More

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January 2020

Neutrophil extracellular traps promote liver micrometastasis in pancreatic ductal adenocarcinoma via the activation of cancer‑associated fibroblasts.

Int J Oncol 2020 Feb 24;56(2):596-605. Epub 2019 Dec 24.

Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812‑8582, Japan.

Cancer‑associated fibroblasts (CAFs) promote the progression of pancreatic ductal adenocarcinoma (PDAC) via tumor‑stromal interactions. Neutrophil extracellular traps (NETs) are extracellular DNA meshworks released from neutrophils together with proteolytic enzymes against foreign pathogens. Emerging studies suggest their contribution to liver metastasis in several types of cancer. Read More

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February 2020

Immunopathology of Kikuchi-Fujimoto disease: A reappraisal using novel immunohistochemistry markers.

Histopathology 2020 Aug 30;77(2):262-274. Epub 2020 Jul 30.

Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TV, USA.

Aims: Kikuchi-Fujimoto disease (KFD) is a self-limited disease characterised by destruction of the lymph node parenchyma. Few studies have assessed the immunohistological features of KFD, and most employed limited antibody panels that lacked many of the novel immunohistochemistry markers currently available.

Methods And Results: We used immunohistochemistry to reappraise the microanatomical distribution of plasmacytoid dendritic cells (pDCs), follicular helper T cells and cytotoxic T cells, B cells, follicular dendritic cell (FDC) meshworks, and histiocytes in lymph nodes involved by KFD. Read More

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The life and death of the germinal center.

Ann Diagn Pathol 2020 Feb 13;44:151421. Epub 2019 Nov 13.

NeoGenomics, Aliso Viejo, CA, United States of America; Department of Hematopathology, M.D. Anderson Cancer Center, Houston, TX, United States of America. Electronic address:

The formation, development and dissolution of germinal centers is a major part of immune system function. It is important to differentiate neoplastic processes from follicular hyperplasia and regressive follicular changes. Better understanding of germinal center development and dissolution also provides diagnostic clues to the underlying pathologic process. Read More

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February 2020

Altered fibrin clot properties and fibrinolysis in patients with atrial fibrillation: practical implications.

Authors:
Anetta Undas

Europace 2020 02;22(2):185-194

Institute of Cardiology, Jagiellonian University School of Medicine and John Paul II Hospital, 80 Prądnicka Str., 31-202 Cracow, Poland.

Compelling evidence indicates that a hypercoagulable state occurs in patients with atrial fibrillation (AF) including those in sinus rhythm following paroxysmal and persistent AF. Activation of blood coagulation in AF reflects heightened thrombin generation with the subsequent increased formation of fibrin as evidenced by elevated soluble fibrin monomers and D-dimer. Formation of denser fibrin meshworks, relatively resistant to plasmin-mediated lysis has been demonstrated in patients with AF. Read More

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February 2020

Application of a 5 Marker Panel to the Routine Diagnosis of Peripheral T-Cell Lymphoma With T-Follicular Helper Phenotype.

Am J Surg Pathol 2019 09;43(9):1282-1290

Division of Hematopathology.

The 2017 World Health Organization update introduced a new category of nodal peripheral T-cell lymphoma with T-follicular helper phenotype (PTCL-TFH) defined by expression of at least 2 or 3 TFH markers. Our study assesses the utility of an immunohistochemical panel of 5 TFH markers (CD10, BCL6, PD-1, CXCL13, and ICOS) for identification of TFH phenotype in angioimmunoblastic T-cell lymphoma (AITL) and PTCL not otherwise specified (NOS). Immunohistochemical for the 5 markers was performed on 22 cases of AITL and 29 cases of PTCL-NOS. Read More

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September 2019

Intussusceptive lymphangiogenesis in the sinuses of developing human foetal lymph nodes.

Ann Anat 2019 Nov 4;226:73-83. Epub 2019 Jul 4.

Department of Basic Medical Sciences, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

A meshwork of intraluminal processes in lymph node (LN) sinuses originates during LN development. Lymph flows through the meshwork, which has an important role in immunology and pathology. However, the formation mechanism of intraluminal processes has not been sufficiently studied. Read More

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November 2019

Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics.

Methods Mol Biol 2019 ;1992:135-149

Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.

The cortical microtubule and actin meshworks play a central role in the shaping of plant cells. Transgenic plants expressing fluorescent protein markers specifically tagging the two main cytoskeletal systems are available, allowing noninvasive in vivo studies. Advanced microscopy techniques, in particular confocal laser scanning microscopy (CLSM), spinning disk confocal microscopy (SDCM), and variable angle epifluorescence microscopy (VAEM), can be nowadays used for imaging the cortical cytoskeleton of living cells with unprecedented spatial and temporal resolution. Read More

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February 2020

On the cause of sleep: Protein fragments, the concept of sentinels, and links to epilepsy.

Proc Natl Acad Sci U S A 2019 05 13;116(22):10773-10782. Epub 2019 May 13.

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125

The molecular-level cause of sleep is unknown. In 2012, we suggested that the cause of sleep stems from cumulative effects of numerous intracellular and extracellular protein fragments. According to the fragment generation (FG) hypothesis, protein fragments (which are continually produced through nonprocessive cleavages by intracellular, intramembrane, and extracellular proteases) can be beneficial but toxic as well, and some fragments are eliminated slowly during wakefulness. Read More

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Telocytes constitute a widespread interstitial meshwork in the lamina propria and underlying striated muscle of human tongue.

Sci Rep 2019 04 10;9(1):5858. Epub 2019 Apr 10.

Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Florence, Italy.

Telocytes have recently emerged as unique interstitial cells defined by their extremely long, thin and moniliform prolongations termed telopodes. Despite growing evidence that these cells consistently reside in the stromal compartment of various organs from human beings, studies dealing with telocytes in structures of the oral cavity are scarce. Hence, the present morphologic study was undertaken to explore for the first time the presence and specific localization of telocytes within tissues of the normal human tongue, a complex muscular organ whose main functions include taste, speech, and food manipulation in the oral cavity. Read More

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Intussusceptive lymphangiogenesis in vascular transformation of lymph node sinuses.

Acta Histochem 2019 May 6;121(4):392-399. Epub 2019 Mar 6.

Department of Basic Medical Sciences, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

Numerous lymphatic anastomosing channels in the lymph nodes are the most demonstrative finding of the rare lesion termed "vascular transformation of lymph node sinuses" (VTS). The mechanism of lymphatic vessel formation in VTS has not been studied. Vessel intussusception contributes to vascular expansion, and intraluminal pillars/posts, interstitial tissue structures or larger pillars (ITSs) and folds are the hallmarks of this process in blood vessels. Read More

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Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting.

Nat Commun 2018 10 25;9(1):4450. Epub 2018 Oct 25.

Pathogenesis of Vascular Infections Unit, INSERM, Institut Pasteur, Paris, 75015, France.

The shape of cellular membranes is highly regulated by a set of conserved mechanisms that can be manipulated by bacterial pathogens to infect cells. Remodeling of the plasma membrane of endothelial cells by the bacterium Neisseria meningitidis is thought to be essential during the blood phase of meningococcal infection, but the underlying mechanisms are unclear. Here we show that plasma membrane remodeling occurs independently of F-actin, along meningococcal type IV pili fibers, by a physical mechanism that we term 'one-dimensional' membrane wetting. Read More

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October 2018

Protease-resistant cell meshworks: An indication of membrane nanotube-based syncytia formation.

Exp Cell Res 2018 11 21;372(2):85-91. Epub 2018 Sep 21.

Department of Neurobiology, Interdisciplinary Centre for Neurosciences (IZN), University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany. Electronic address:

Cell biology considers most animal tissues as assemblies of "individual" cells that rely on different contact-dependent communication mechanisms, including synapses, gap junctions or - a recent awareness - membrane nano- and microtubes. However, by protease-mediated singularization of dense 2D/ 3D cell cultures and tissue explants, we show here that cell collectives stay connected via a continuous meshwork of F-actin-based membrane tubes, resembling tunneling nanotube (TNT)-based networks observed between dispersed cell cultures. Fusion of respective tubes was accompanied by the ingrowth of microtubules and the invasion of mitochondria and lysosomes. Read More

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November 2018

Life lines: Loss, loneliness and expanding meshworks with an urban Walk and Talk group.

Health Place 2018 09 23;53:164-172. Epub 2018 Aug 23.

School of Psychology, University of East London, Water Lane, Stratford, London E15 4LZ, United Kingdom. Electronic address:

There is ample evidence that loneliness and social isolation are important in the development of distress, and harmful to recovery. UK mental health services, however, have been moving away from interventions which prioritise relationality, and towards professionally-led, individualised treatments. In addition, those experiencing distress experience multiple spatial exclusions which can compound isolation and loneliness. Read More

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September 2018

Topological remodeling of cortical perineuronal nets in focal cerebral ischemia and mild hypoperfusion.

Matrix Biol 2018 12 6;74:121-132. Epub 2018 Aug 6.

Department of Neurology, University Hospital Essen, Hufelandstraße 55, D-45122 Essen, Germany. Electronic address:

Despite the crucial role of perineuronal nets (PNNs) in neural plasticity and neurological disorders, their ultrastructural organization remains largely unresolved. We have developed a novel approach combining superresolution structured illumination microscopy (SR-SIM) and mathematical reconstruction that allows for quantitative analysis of PNN topology. Since perineuronal matrix is capable to restrict neural plasticity but at the same time is necessary to maintain synapses, we hypothesized that a beneficial post stroke recovery requires a reversible loosening of PNNs. Read More

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December 2018

Congenital hypodysfibrinogenemia associated with a novel deletion of three residues (γAla289_Asp291del) in fibrinogen.

J Thromb Thrombolysis 2018 Aug;46(2):211-218

Department of Internal Medicine, The First Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, 325000, China.

Hypodysfibrinogenemia is the least frequently reported congenital fibrinogen disorder, characterized by both quantity and quality defects of fibrinogen. In this study, we investigated the molecular basis of hypodysfibrinogenemia in a Chinese family. Functional fibrinogen was measured by Clauss method, and the antigenic fibrinogen was measured by immunoturbidimetry assay. Read More

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Angioimmunoblastic T-cell lymphoma: a rare subtype of peripheral T-cell lymphoma.

Clin Case Rep 2018 04 10;6(4):750-752. Epub 2018 Feb 10.

Michigan State University/Sparrow hospital B301, 788 Service Road East Lansing Michigan 48824.

Angioimmunoblastic T-cell lymphoma (AITL) is a rare form of NHL and usually presents in the late stage due to the atypical laboratory findings. Immunohistochemistry of the lymph node in AITL is characterized by positive CD2, CD3, CD4, CD10, CXCL-13, PD1 often BCL-6 and CD20 positive. Meshworks of follicular dendritic cells are seen outside follicles with CD21 and CD23 stains. Read More

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Neutrophil extracellular traps promote lipopolysaccharide-induced airway inflammation and mucus hypersecretion in mice.

Oncotarget 2018 Mar 8;9(17):13276-13286. Epub 2018 Jan 8.

Department of Geriatrics, Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, China.

Bacterial lipopolysaccharide (LPS) contributes to airway inflammation and mucus hypersecretion in chronic airway inflammatory diseases, such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF). Neutrophil extracellular traps (NETs) are extracellular meshworks composed of DNA fibers and antimicrobial proteins. Although NET formation has been detected in COPD and CF patients, how NETs contribute to these diseases is poorly understood. Read More

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Human LINE-1 retrotransposition requires a metastable coiled coil and a positively charged N-terminus in L1ORF1p.

Elife 2018 03 22;7. Epub 2018 Mar 22.

Department of Biochemistry, Max Planck Institute for Developmental Biology, Tübingen, Germany.

LINE-1 (L1) is an autonomous retrotransposon, which acted throughout mammalian evolution and keeps contributing to human genotypic diversity, genetic disease and cancer. L1 encodes two essential proteins: L1ORF1p, a unique RNA-binding protein, and L1ORF2p, an endonuclease and reverse transcriptase. L1ORF1p contains an essential, but rapidly evolving N-terminal portion, homo-trimerizes via a coiled coil and packages L1RNA into large assemblies. Read More

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Involvement of 11-ketotestosterone in hooknose formation in male pink salmon (Oncorhynchus gorbuscha) jaws.

Gen Comp Endocrinol 2018 05 17;260:41-50. Epub 2018 Feb 17.

Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan.

Mature male Pacific salmon (Oncorhynchus spp.) develop a hooknose, as a secondary male sexual characteristic, during the spawning period. It is likely that androgens regulate hooknose formation. Read More

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