87,870 results match your criteria membranes time

Dinoprostone vaginal insert (DVI) versus adjunctive sweeping of membranes and DVI for term induction of labor.

J Obstet Gynaecol Res 2021 Jun 23. Epub 2021 Jun 23.

Department of Obstetrics & Gynaecology, St George's University of London, London, UK.

Aim: To compare the efficacy and safety of dinoprostone vaginal insert (DVI) alone versus DVI with adjunctive sweeping of membranes (ASM) for induction of labor (IOL).

Methods: Single-center, prospective, randomized controlled trial; women with singleton term pregnancies, cervical dilation ≥1 and <3 cm, intact membranes allocated to either DVI or DVI with ASM. The primary outcome was vaginal delivery within 24 h of insertion. Read More

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Clinical, randomized, double blind clinical trial to study the effect of parenteral supplementation with fish oil emulsion in the nutritional support in esophagectomized patients.

Medicine (Baltimore) 2021 Jun;100(25):e26426

Pharmacy Department.

Introduction: Esophagectomy is a major surgery with a high degree of catabolic and post-surgical inflammatory response accompanied by high morbidity and significant mortality. Post-surgical nutritional support via enteral administration of ω-3 fatty acids has been seen to be effective although its bad tolerance. There are few clinical trials with parenteral ω-3 fatty acids in these patients. Read More

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Ion track etching of polycarbonate membranes monitored by small angle X-ray scattering.

Phys Chem Chem Phys 2021 Jun 23. Epub 2021 Jun 23.

Department of Electronic Materials Engineering, Research School of Physics, Australian National University, Canberra ACT 2601, Australia.

In situ small angle X-ray scattering (SAXS) measurements of ion track etching in polycarbonate foils are used to directly monitor the selective dissolution of ion tracks with high precision, including the early stages of etching. Detailed information about the track etching kinetics and size, shape, and size distribution of an ensemble of nanopores is obtained. Time resolved measurements as a function of temperature and etchant concentration show that the pore radius increases almost linearly with time for all conditions and the etching process can be described by an Arrhenius law. Read More

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Extraction of Pregabalin in Urine Samples Using a Sulfonated Poly(ether ether ketone) Membrane.

Int J Anal Chem 2021 31;2021:3439242. Epub 2021 May 31.

Department of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi Arabia.

In this work, a simple polymer-assisted microextraction technique was developed to determine pregabalin (an anticonvulsant drug) in the urine sample. A sulfonated poly(ether ether ketone) membrane was used as a sorbent for pregabalin extraction, and the extraction performance was compared with that of the conventional polydimethylsiloxane membrane. The extraction device is free moving and tumbles continuously throughout the stirred sample solution during extraction to enhance the extraction efficiency. Read More

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Wiad Lek 2021 ;74(4):821-827


Objective: The aim is to identify the morphological features of the uterus layers in women at different time intervals of the postmortem period as diagnostic criteria for establishing the postmortem interval.

Patients And Methods: Materials and methods: In the study we used surgical and autopsy material - uterine tissue fragments. All materials were divided into two groups. Read More

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Low-dose oral misoprostol for induction of labour.

Cochrane Database Syst Rev 2021 Jun 22;6:CD014484. Epub 2021 Jun 22.

Department of Women's and Children's Health, The University of Liverpool, Liverpool, UK.

Background: Misoprostol given orally is a commonly used labour induction method. Our Cochrane Review is restricted to studies with low-dose misoprostol (initially ≤ 50 µg), as higher doses pose unacceptably high risks of uterine hyperstimulation.

Objectives: To assess the efficacy and safety of low-dose oral misoprostol for labour induction in women with a viable fetus in the third trimester of pregnancy. Read More

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Induction within or after 12 hours of ≥ 36 weeks prelabor rupture of membranes: a systematic review and meta-analysis.

Am J Obstet Gynecol MFM 2021 Jun 18:100425. Epub 2021 Jun 18.

Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Sidney Kimmel Medical College of Thomas Jefferson University, Philadelphia, PA, USA. Electronic address:

Objective: To evaluate the incidence of chorioamnionitis in women with singleton gestations with ≥36 weeks prelabor rupture of membranes (PROM) induced with oxytocin within or after 12 hours or PROM.

Data Sources: The search was conducted using MEDLINE, Web of Sciences, Scopus, ClinicalTrial.gov, OVID and Cochrane Library as electronic databases from their inception until May 2020. Read More

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Fabrication and performance evaluation of polymeric membrane using blood compatible hydroxyapatite for artificial kidney application.

Artif Organs 2021 Jun 21. Epub 2021 Jun 21.

Department of Chemistry, University of Gujrat, Punjab, Pakistan.

In the current study, a phase inversion scheme was employed to fabricate hydroxyapatite (HA)/Polysulfone (PSF) based asymmetric membranes through using a film applicator with water as solvent-nonsolvent exchanging medium. Fourier Transform Infrared (FTIR) and X-Ray Diffraction (XRD) spectroscopic studies were utilized to confirm the bonding chemistry and purity of filler. The inherent thick nature of PSF generated sponge-like shape while the instantaneous de-mixing process produced finger-like pore networks in HA/PSF-based asymmetric membranes as exhibited by Scanning Electron Microscope (SEM) micrographs. Read More

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Localization of PIP5Kγ selectively in proprioceptive peripheral fields and also in sensory ganglionic satellite cells as well as neuronal cell membranes and their central terminals.

J Anat 2021 Jun 20. Epub 2021 Jun 20.

Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

Based on a previous study by others reporting that PIP5Kγ (phosphatidylinositol 4-phosphate 5-kinase γ) and its product, phosphatidylinositol 4,5 bisphosphate (PIP ), are involved in the regulation of nociception, the present immunohistochemical study examined the localization of PIP5Kγ-immunoreactivity in dorsal root ganglia (DRG) and their peripheral and central terminal fields. PIP5Kγ-immunoreactivity was localized for the first time in the muscle spindles, in which it was found in I-bands of polar regions of intrafusal muscle fibers and also in sensory nerve terminals abutting on equatorial regions of the muscle fibers. This finding indicates the involvement of PIP5Kγ in the proprioception and suggests somehow complicated mechanisms of its involvement because of its heterogeneous localization in intra-I-band structures. Read More

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Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein Expression.

Front Bioeng Biotechnol 2021 4;9:679448. Epub 2021 Jun 4.

Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, United Kingdom.

Transient gene expression (TGE) in mammalian cells is a method of rapidly generating recombinant protein material for initial characterisation studies that does not require time-consuming processes associated with stable cell line construction. High TGE yields are heavily dependent on efficient delivery of plasmid DNA across both the plasma and nuclear membranes. Here, we harness the protein nucleoside diphosphate kinase (NDPK-A) that contains a nuclear localisation signal (NLS) to enhance DNA delivery into the nucleus of CHO cells. Read More

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Comparing the performance of the conventional and fixed-bed membrane bioreactors for treating municipal wastewater.

J Environ Health Sci Eng 2021 Jun 24;19(1):997-1004. Epub 2021 Apr 24.

Department of Environmental Health Engineering, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Membrane bioreactor (MBR) is relatively a new technology in wastewater treatment. It can efficiently remove soluble and suspended organics. However, it may constantly encounter bio-fouling and cannot efficiently remove nutrient pollutants. Read More

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Isolation Method and Characterization of Outer Membranes Vesicles of Grown in a Chemically Defined Medium.

Front Microbiol 2021 2;12:654193. Epub 2021 Jun 2.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

Outer membrane vesicles (OMVs) are small vesicles constitutively shed by all Gram-negative bacterium, which have been proposed to play a role in persistence and pathogenesis. The methods currently available for the isolation of OMVs are diverse and time-consuming, raising the need for a protocol standardization, which was the main aim of this study. Here, we showed that the chemically defined F12 medium, supplemented with cholesterol, nutritionally supports bacterial growth and maintains viability for at least 72 h. Read More

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Contact probing of prestressed adhesive membranes of living cells.

Philos Trans A Math Phys Eng Sci 2021 Aug 21;379(2203):20200289. Epub 2021 Jun 21.

School of Engineering, Cardiff University, Cardiff, UK.

Atomic force microscopy (AFM) studies of living biological cells is one of main experimental tools that enable quantitative measurements of deformation of the cells and extraction of information about their structural and mechanical properties. However, proper modelling of AFM probing and related adhesive contact problems are of crucial importance for interpretation of experimental data. The Johnson-Kendall-Roberts (JKR) theory of adhesive contact has often been used as a basis for modelling of various phenomena including cell-cell interactions. Read More

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Testosterone synthesis in testicular Leydig cells after long-term exposure to a static electric field (SEF).

Toxicology 2021 Jun 17:152836. Epub 2021 Jun 17.

Institute of Environmental Process, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, PR China.

China's clean energy and resources are mainly located in the west and north while electric load center is concentrated in the middle and east. Thus, these resources and energy need to be converted into electrical energy in situ and transported to electric load center through ultra-high voltage direct current (UHVDC) transmissions. China has built 25000 km UHVDC transmission lines of 800 kV and 1100 kV, near which the impact of electric field on health has attracted public attention. Read More

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Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.

Colloids Surf B Biointerfaces 2021 Jun 8;205:111916. Epub 2021 Jun 8.

Institut Galien Paris Saclay, CNRS UMR 8612, Université Paris-Saclay, Faculté de Pharmacie, 5 rue J-B Clément, 92296, Châtenay-Malabry, France. Electronic address:

Increasing valence by acting on nanomaterial morphology can enhance the ability of a ligand to specifically bind to targeted cells. Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions. Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners. Read More

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An insight into the mechanism of antibacterial activity by magnesium oxide nanoparticles.

J Mater Chem B 2021 Jun 18. Epub 2021 Jun 18.

Indian Institute of Technology Kharagpur, 721302, India.

The exact mechanism behind the antibacterial efficacy of nanoparticles has remained unexplored to date. This study aims to shed light the mechanism adopted using magnesium oxide nanoparticles prepared in ethyl alcohol against gram-negative and gram-positive bacterial cells, and the generation of reactive oxygen species (ROS) is proposed to be the dominant mechanism. This paradigm is supported by the quantification of the hydroxyl radical and superoxide anions produced in the nanoparticle treated and untreated bacterial solutions, and by the reduction of the antibacterial efficiency after the addition of a radical scavenger. Read More

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Cell-derived biomimetic nanocarriers for targeted cancer therapy: cell membranes and extracellular vesicles.

Drug Deliv 2021 Dec;28(1):1237-1255

Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai, China.

Nanotechnology provides synthetic carriers for cancer drug delivery that protect cargos from degradation, control drug release and increase local accumulation at tumors. However, these non-natural vehicles display poor tumor targeting and potential toxicity and are eliminated by the immune system. Recently, biomimetic nanocarriers have been widely developed based on the concept of 'mimicking nature. Read More

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December 2021

Preparation of lignosulfonate-based nanofiltration membranes with improved water desalination performance.

Eng Life Sci 2021 Jun 2;21(6):417-428. Epub 2021 Apr 2.

Beijing Key Laboratory of Lignocellulosic Chemistry Beijing Forestry University Beijing P. R. China.

Pulping and papermaking generate large amounts of waste in the form of lignosulfonates which have limited valorized applications so far. Herein, we report a novel lignosulfonate-based nanofiltration membrane, prepared by using polyethylenimine (PEI) and sodium lignosulfonate (SL) via a layer-by-layer (LbL) self-assembly. As a low-cost and renewable natural polyelectrolyte, SL is selected to replace the synthetic polyelectrolyte commonly used in the conventional LbL fabrication for composite membranes. Read More

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Nanofiltration membrane for bio-separation: Process-oriented materials innovation.

Eng Life Sci 2021 Jun 20;21(6):405-416. Epub 2021 Mar 20.

State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.

Nanofiltration (NF) with advantages of high efficiency and low-cost has attracted increasing attentions in bio-separation. However, the large-scale application is limited by the inferior molecular selectivity, low chemical stability and serious membrane fouling. Many efforts, thus, have been devoted in NF materials design for specific applications to enhance the separation efficiency of bio-products and increase membrane life-time, as well as reduce the operating cost. Read More

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Ginsenoside Rg1 can restore hematopoietic function by inhibiting Bax translocation-mediated mitochondrial apoptosis in aplastic anemia.

Sci Rep 2021 Jun 17;11(1):12742. Epub 2021 Jun 17.

Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250014, China.

The present study investigated, the anti-apoptotic activity of Ginsenoside Rg1 (Rg1) via inhibition of Bax translocation and the subsequent recovery of hematopoietic function. Mitochondrial apoptosis in bone marrow mononuclear cells (BMNCs) was observed in aplastic anemia (AA) patients. To establish a mouse model of AA, BALB/c mice were transplanted with lymph node cells from DBA/2 donor mice via vein injection after treatment with Co60 γ-radiation. Read More

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Recent advances in integrated solid-state nanopore sensors.

Lab Chip 2021 Jun 17. Epub 2021 Jun 17.

School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064 USA.

The advent of single-molecule probing techniques has revolutionized the biomedical and life science fields and has spurred the development of a new class of labs-on-chip based on powerful biosensors. Nanopores represent one of the most recent and most promising single molecule sensing paradigms that is seeing increased chip-scale integration for improved convenience and performance. Due to their physical structure, nanopores are highly sensitive, require low sample volume, and offer label-free, amplification-free, high-throughput real-time detection and identification of biomolecules. Read More

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Four-dimensional imaging and free-energy analysis of sudden pore-filling events in wicking of yarns.

Phys Rev E 2021 May;103(5-1):053101

Chair of Building Physics, Swiss Federal Institute of Technology Zurich (ETHZ), Stefano-Franscini-Platz 5, 8093 Zurich, Switzerland.

What are the mechanisms at play in the spontaneous imbibition dynamics in polyethylene terephthalate filament yarns at pore scale? Processes at pore scale such as waiting times between the filling of two neighboring pores, as observed in special irregular porous media, like yarns, may overrule the predicted behavior by well-known laws such as Washburn's law. While the imbibition physics are well known, classic models like Washburn's law cannot explain the dynamics observed for yarns. The stepwise dynamics is discussed in terms of the interplay of thermodynamic free energy and viscous dissipation. Read More

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Non-thermal plasma-based inactivation of bacteria in water using a microfluidic reactor.

Water Res 2021 Jun 4;201:117321. Epub 2021 Jun 4.

Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom. Electronic address:

Failure of conventional water treatment systems may lead to the contamination of water sources, which can cause outbreaks of waterborne healthcare associated infections. Advanced oxidation processing by non-thermal plasma has the potential to treat water without the addition of chemicals. Antibiotic resistant Pseudomonas aeruginosa and Escherichia coli were chosen to investigate the use of non-thermal plasma generated in a microfluidic reactor to disinfect bacteria contaminated water. Read More

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[Clinical features of very preterm infants with prelabor rupture of membranes and predictive factors for major adverse outcomes].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Jun;23(6):575-581

Department of Neonatology, Third Affiliated Hospital of Zhengzhou University/Henan Key Laboratory of Child Brain Injury/Henan Pediatric Clinical Research Center, Zhengzhou 450052, China.

Objective: To study the clinical features of very preterm infants with prelabor rupture of membranes (PROM) and predictive factors for early-onset sepsis (EOS) and death.

Methods: A retrospective analysis was performed for the clinical data of the very preterm infants with PROM (with a gestational age of < 32 weeks) who were admitted to the neonatal intensive care unit from January 2018 to May 2020. According to the time from membrane rupture to delivery, the infants were divided into four groups: < 18 hours (=107), 18 hours to < 3 days (=111), 3 days to < 14 days (=144), and ≥ 14 days (=37). Read More

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Risk Factors Associated with Preterm Prelabor Rupture of Membranes after Cord Occlusion in Monochorionic Diamniotic Twins.

Fetal Diagn Ther 2021 Jun 15:1-7. Epub 2021 Jun 15.

BCNatal Fetal Medicine Research Center, Hospital Clínic and Hospital Sant Joan de Déu, University of Barcelona, Barcelona, Spain.

Introduction: Preterm prelabor rupture of membranes (PPROM) is a common complication after fetal surgeries. The aim of this study was to assess risk factors for and outcomes after PPROM following cord occlusion (CO) in monochorionic diamniotic (MCDA) pregnancies.

Methods: This was a retrospective cohort study of 188 consecutive MCDA pregnancies treated by bipolar or laser CO, either primarily because of discordant malformation (dMF) or severe selective fetal growth restriction (sFGR), or secondarily when complete bichorionization was not possible in case of twin-to-twin transfusion syndrome (TTTS) or sFGR. Read More

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Overcoming the Trade-off between Water Permeation and Mechanical Strength of Ceramic Membrane Supports by Interfacial Engineering.

ACS Appl Mater Interfaces 2021 Jun 14. Epub 2021 Jun 14.

Department of Material Science and Engineering, Faculty of Engineering, National University of Singapore, Singapore 117574, Singapore.

Porous ceramic membrane supports with high mechanical strength and permeation are required for highly permeable ceramic membranes. The water permeation of a ceramic membrane support is largely dependent on its level of open porosity, which can be however generally detrimental to the mechanical strength. In this work, low-cost kaolin nanoflakes were rationally composited with coarse alumina particles, and multichannel flat-sheet ceramic supports were successfully fabricated by extrusion and subsequent partial sintering. Read More

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Evaluation of the hybrid system combining electrocoagulation, nanofiltration and reverse osmosis for biologically treated textile effluent: Treatment efficiency and membrane fouling.

J Environ Manage 2021 Jun 12;294:113042. Epub 2021 Jun 12.

Istanbul University-Cerrahpaşa, Faculty of Engineering, Department of Environmental Engineering, Avcilar Campus, Avcilar, 34320, Istanbul, Turkey. Electronic address:

The efficiency of the hybrid electrocoagulation-nanofiltration-reverse osmosis (EC-NF-RO) system for the treatment of biologically treated textile effluent was investigated. The treatment performances and membrane fouling behaviours of nanofiltration (NF) and hybrid EC-NF systems were compared. EC process was evaluated concerning mitigate the membrane fouling and increasing the removal efficiencies. Read More

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Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion.

Methods 2021 Jun 11. Epub 2021 Jun 11.

Central Laser Facility, Rutherford Appleton Laboratory, MRC-Research Complex at Harwell, Science and Technology Facilities Council, Harwell, OX11 0QX UK; National Heart and Lung Institute, Imperial College London, Sir Alexander Fleming Building, London, SW7 2AZ UK; NIHR Imperial Biomedical Research Centre, London, SW7 2AZ UK. Electronic address:

The primary cilium is a specialized plasma membrane protrusion with important receptors for signalling pathways. In polarized epithelial cells, the primary cilium assembles after the midbody remnant (MBR) encounters the centrosome at the apical surface. The membrane surrounding the MBR, namely remnant associated membrane patch (RAMP), once situated next to the centrosome, releases some of its lipid components to form a centrosome-associated membrane patch (CAMP) from which the ciliary membrane stems. Read More

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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique.

J Vis Exp 2021 May 26(171). Epub 2021 May 26.

Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka;

The Ussing chamber technique was first invented by the Danish scientist Hans Ussing in 1951 to study the transcellular transport of sodium across frog skin. Since then, this technique has been applied to many different tissues to study the physiological parameters of transport across membranes. The Ussing chamber method is preferable to other methods because native tissue can be used, making it more applicable to what is happening in vivo. Read More

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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions.

J Vis Exp 2021 May 27(171). Epub 2021 May 27.

Department of Physics, Virginia Tech; Center for Soft Matter and Biological Physics, Virginia Tech;

Lipid bilayers form the main matrix of cell membranes and are the primary platform for nutrient exchange, protein-membrane interactions, and viral budding, among other vital cellular processes. For efficient biological activity, cell membranes should be rigid enough to maintain the integrity of the cell and its compartments yet fluid enough to allow membrane components, such as proteins and functional domains, to diffuse and interact. This delicate balance of elastic and fluid membrane properties, and their impact on biological function, necessitate a better understanding of collective membrane dynamics over mesoscopic length and time scales of key biological processes, e. Read More

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