62,007 results match your criteria membrane increasing

2D confinement freestanding graphene oxide composite membranes with enriched oxygen vacancies for enhanced organic contaminants removal via peroxymonosulfate activation.

J Hazard Mater 2021 May 8;417:126028. Epub 2021 May 8.

Institute of Green Chemistry and Chemical Technology, Advanced Chemical Engineering Laboratory of Green Materials and Energy of Jiangsu Province, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:

Introducing membrane filtration into advanced oxidation processes to decrease energy and cost consumption has been considered as a promising direction in environmental remediation. In this work, we firstly developed a kind of novel lawn-like FeO@CoFe@nitrogen-doped reduced graphene oxide@carbon nanotube composites (FeCo@GCTs) through in-situ pyrolysis of self-assembly of Prussian blue analogues and GO, followed through a vacuum-assisted filtration strategy to fabricate 2D confinement freestanding GO composite membrane. Electrochemical analysis and H-TPR revealed the superiority of FeCo@GCTs as ideal electron acceptor, and this unique lawn-like structure concentrated active sites with a confined space and enriched oxygen vacancies that realized 98. Read More

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Differences in kinetic metabolomics in Eisenia fetida under single and dual exposure of imidacloprid and dinotefuran at environmentally relevant concentrations.

J Hazard Mater 2021 May 8;417:126001. Epub 2021 May 8.

Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China. Electronic address:

Metabolomic responses of earthworms to neonicotinoids are important for understanding their molecular-level toxicity and assessing their ecological risks, but little is known until now. We investigated impact of imidacloprid (IMI, 52.6 ng/g) and dinotefuran (DIN, 52. Read More

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Reduced Th1 response is associated with lower glycolytic activity in activated peripheral blood mononuclear cells after metabolic and bariatric surgery.

J Endocrinol Invest 2021 May 15. Epub 2021 May 15.

Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Cátedra de Cardiología y Medicina Vascular, Ave. Morones Prieto 3000, 64710, Monterrey, NL, Mexico.

Background: Obesity promotes cellular immunometabolism changes that trigger the activation of macrophages and lymphocytes, leading to systemic inflammation. Activated leukocytes undergo metabolic reprogramming, increasing glycolytic activity.

Objective: To examine whether the reduction in the inflammatory state associated with bariatric surgery is associated with decreased glycolytic activity in leukocytes. Read More

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Metabolic Flux Analysis of Simultaneous Production of Vitamin B and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii.

Appl Biochem Biotechnol 2021 May 15. Epub 2021 May 15.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

The metabolic processes involved in simultaneous production of vitamin B and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanism of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. Read More

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Structural traits of leaf epidermis correspond to metal tolerance in Rumex acetosella populations growing on metal-contaminated soils.

Protoplasma 2021 May 15. Epub 2021 May 15.

Cell Imaging and Ultrastructure Research, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria.

The pseudometallophyte Rumex acetosella L. occupies habitats with normal and high soil concentrations of zinc (Zn), lead (Pb), and copper (Cu). It remains unclear if the plants respond to the toxic metals by altering their morphology and increasing the resilience of their cells. Read More

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A spatial model of YAP/TAZ signaling reveals how stiffness, dimensionality, and shape contribute to emergent outcomes.

Proc Natl Acad Sci U S A 2021 May 14;118(20). Epub 2021 May 14.

Mechanical and Aerospace Engineering, Jacobs School of Engineering, University of California San Diego, La Jolla, CA 92093

YAP/TAZ is a master regulator of mechanotransduction whose functions rely on translocation from the cytoplasm to the nucleus in response to diverse physical cues. Substrate stiffness, substrate dimensionality, and cell shape are all input signals for YAP/TAZ, and through this pathway, regulate critical cellular functions and tissue homeostasis. Yet, the relative contributions of each biophysical signal and the mechanisms by which they synergistically regulate YAP/TAZ in realistic tissue microenvironments that provide multiplexed input signals remain unclear. Read More

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Adverse birth outcomes and associated factors among mothers who delivered in Bale zone hospitals, Oromia Region, Southeast Ethiopia.

J Int Med Res 2021 May;49(5):3000605211013209

Department of Anesthesia, School of Medicine, Negele Arsi General Hospital and Medical College, Shashemene, Ethiopia.

Objective: Adverse birth outcomes, which include stillbirth, preterm birth, low birthweight, congenital abnormalities, and stillbirth, are the leading cause of neonatal and infant mortality worldwide. We assessed adverse birth outcomes and associated factors among mothers who gave birth in Bale zone hospitals, Oromia, Southeast Ethiopia.

Methods: We used systematic random sampling in this cross-sectional study. Read More

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The role of hopanoids in fortifying rhizobia against a changing climate.

Environ Microbiol 2021 May 14. Epub 2021 May 14.

Division of Biology & Biological Engineering, California Institute of Technology, 1200 E California Blvd, Pasadena, CA, 91125, USA.

Bacteria are a globally sustainable source of fixed nitrogen, which is essential for life and crucial for modern agriculture. Many nitrogen-fixing bacteria are agriculturally important, including bacteria known as rhizobia that participate in growth-promoting symbioses with legume plants throughout the world. To be effective symbionts, rhizobia must overcome multiple environmental challenges: from surviving in the soil, to transitioning to the plant environment, to maintaining high metabolic activity within root nodules. Read More

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Automatic segmentation of retinal layers in OCT images with intermediate age-related macular degeneration using U-Net and DexiNed.

PLoS One 2021 14;16(5):e0251591. Epub 2021 May 14.

Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Age-related macular degeneration (AMD) is an eye disease that can cause visual impairment and affects the elderly over 50 years of age. AMD is characterized by the presence of drusen, which causes changes in the physiological structure of the retinal pigment epithelium (RPE) and the boundaries of the Bruch's membrane layer (BM). Optical coherence tomography is one of the main exams for the detection and monitoring of AMD, which seeks changes through the evaluation of successive sectional cuts in the search for morphological changes caused by drusen. Read More

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Effect of Glutathione Supplementation to Semen Extender on Post-thawed Rooster Sperm Quality Indices Frozen After Different Equilibration Times.

Cryo Letters 2020 Mar-Apr;41(2):92-99

Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Background: Avian sperm is susceptible to lipid peroxidation, compromising their fertility. The semen antioxidant system protects sperm plasma membrane against reactive oxygen species.

Objective: The study evaluates the effect of glutathione (GSH) addition to semen extender during different equilibration times (ET) on rooster sperm cryopreservation. Read More

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Survival and Outcomes after Cardiac Arrest with VA-ECMO Rescue Therapy.

Shock 2021 May 13. Epub 2021 May 13.

ECMO Research Group, Division of Cardiothoracic Surgery, Department of Surgery, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY USA.

Background: Extracorporeal membrane oxygenation (ECMO) use in patients with cardiac arrest is increasing. Utilization remains variable between centers using ECMO as a rescue therapy or early protocolized extracorporeal cardiopulmonary resuscitation.

Methods: Single-center, retrospective evaluation of cardiac arrest with cardiopulmonary resuscitation and rescue ECMO support from 2011 through 2019. Read More

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Template-Free Formation of Regular Macroporosity in Carbon Materials Made from a Folded Polymer Precursor.

Small 2021 May 13:e2100712. Epub 2021 May 13.

Schulich Faculty of Chemistry and the Grand Technion Energy Program, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

Porous carbon materials attract great interest in a wide range of applications such as batteries, fuel cells, and membranes, due to their large surface area, structural and compositional tunability, and chemical stability. While micropores are typically obtained when preparing carbon materials by pyrolysis, the fabrication of mesoporous, and especially macroporous carbons is more challenging, yet important for enhancing mass transport. Herein, template-free regular macroporous carbons are prepared from a mixture of unfolded (linear) and folded (single-chain nanoparticles, SCNP) polyvinylpyrrolidone chains. Read More

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Effects of Lipids and Surfactants on the Fermentation Production of Echinocandin B by Aspergillus nidulans.

J Appl Microbiol 2021 May 13. Epub 2021 May 13.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.

Aims: Echinocandin B (ECB) is a kind of lipopeptide antifungal antibiotic, as well as the key precursor of antifungal drug Anidulafungin. Its efficient bioproduction plays an important role in promoting the industrial production of Anidulafungin.

Methods And Results: In this study, methyl oleate and Tween 80 were firstly used to enhance the ECB fermentation by Aspergillus nidulans, the results showed that the ECB titer was significantly enhanced with the addition of methyl oleate and Tween 80. Read More

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extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa.

J Anim Sci Technol 2021 Jan 31;63(1):58-68. Epub 2021 Jan 31.

Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu 44618, Nepal.

Several herbs including are known to possess conceptive property. In the present study, mouse spermatozoa were incubated with ethanol extract of leaves. The effect of extract on acrosome exocytosis was studied by labeling spermatozoa with fluorescein isothiocyanate (FITC) peanut agglutinin and by staining with Coomassie blue. Read More

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January 2021

Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit αδ-1 but not αδ-3.

Sci Rep 2021 May 13;11(1):10256. Epub 2021 May 13.

Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK.

N-type voltage-gated calcium channels (Ca2.2) are predominantly expressed at presynaptic terminals, and their function is regulated by auxiliary αδ and β subunits. All four mammalian αδ subunits enhance calcium currents through Ca1 and Ca2 channels, and this increase is attributed, in part, to increased Ca expression at the plasma membrane. Read More

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Membrane Remodeling by DNA Origami Nanorods: Experiments Exploring the Parameter Space for Vesicle Remodeling.

Langmuir 2021 May 13. Epub 2021 May 13.

Department of Physics, University of Massachusetts Amherst, Hasbrouck Lab, 666 North Pleasant Street, Amherst, Massachusetts 01002, United States.

Inspired by the ability of cell membranes to alter their shape in response to bound particles, we report an experimental study of long, slender nanorods binding to lipid bilayer vesicles and altering the membrane shape. Our work illuminates the role of particle concentration, adhesion strength, and membrane tension in determining the membrane morphology. We combined giant unilamellar vesicles with oppositely charged nanorods, carefully tuning the adhesion strength, membrane tension, and particle concentration. Read More

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Controlling and Optimizing Photoinduced Charge Transfer across Ultrathin Silica Separation Membrane with Embedded Molecular Wires for Artificial Photosynthesis.

ACS Appl Mater Interfaces 2021 May 13. Epub 2021 May 13.

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, United States.

Ultrathin amorphous silica membranes with embedded organic molecular wires (oligo(-phenylenevinylene), three aryl units) provide chemical separation of incompatible catalytic environments of CO reduction and HO oxidation while maintaining electronic and protonic coupling between them. For an efficient nanoscale artificial photosystem, important performance criteria are high rate and directionality of charge flow. Here, the visible-light-induced charge flow from an anchored Ru bipyridyl light absorber across the silica nanomembrane to CoO water oxidation catalyst is quantitatively evaluated by photocurrent measurements. Read More

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Characterization of the multidrug efflux transporter styMdtMfrom Salmonella enterica serovar Typhi.

Proteins 2021 May 13. Epub 2021 May 13.

Drug Discovery and Structural Biology Group, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.

Salmonellae are foodborne pathogens and the major cause of gastroenteritis in humans. Salmonellae express multidrug efflux transporters that play a key role in their drug resistance, which is becoming an increasing problem for therapeutic intervention. Despite their biomedical importance, the mechanisms underlying substrate transport by multidrug efflux transporters remain poorly understood. Read More

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New advances in brain-targeting nano-drug delivery systems for Alzheimer's disease.

J Drug Target 2021 May 13:1-67. Epub 2021 May 13.

Xiangya School of Pharmaceutical Sciences in Central South University, Changsha 410013, Hunan, China.

Alzheimer's disease (AD) is the most common neurodegenerative disease worldwide and its incidence is increasing due to the aging population. Currently, the main limitations of AD treatment are low blood-brain barrier permeability, severe off-target of drugs, and immune abnormality. In this review, four hypotheses for Alzheimer's pathogenesis and three challenges for Alzheimer's drug delivery are discussed. Read More

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Isoquercitrin protects HUVECs against high glucose‑induced apoptosis through regulating p53 proteasomal degradation.

Int J Mol Med 2021 Jul 13;48(1). Epub 2021 May 13.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

High glucose (HG)‑induced endothelial apoptosis serves an important role in the vascular dysfunction associated with diabetes mellitus (DM). It has been reported that isoquercitrin (IQC), a flavonoid glucoside, possesses an anti‑DM effect, but the mechanism requires further investigation. The present study investigated the effect of IQC against HG‑induced apoptosis in human umbilical vein endothelial cells (HUVECs) and explored its molecular mechanism. Read More

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Scorpion Venom Antimicrobial Peptides Induce Siderophore Biosynthesis and Oxidative Stress Responses in Escherichia coli.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, United Kingdom

The increasing development of microbial resistance to classical antimicrobial agents has led to the search for novel antimicrobials. Antimicrobial peptides (AMPs) derived from scorpion and snake venoms offer an attractive source for the development of novel therapeutics. Smp24 (24 amino acids [aa]) and Smp43 (43 aa) are broad-spectrum AMPs that have been identified from the venom gland of the Egyptian scorpion and subsequently characterized. Read More

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Blocking FcγRIIB in Smooth Muscle Cells Reduces Hypertension.

Circ Res 2021 May 13. Epub 2021 May 13.

Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, CHINA.

Hypertension is a common chronic disease and its prevalence is increasing. Immunoglobulins (Igs) play crucial roles in adaptive immune response and have been implicated in the pathogenesis of hypertension. However, which Igs and receptors as well as underlying mechanisms participating in hypertension remains unknown. Read More

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Dual-Targeting Antiproliferation Hybrids Derived from 1-Deoxynojirimycin and Kaempferol Induce MCF-7 Cell Apoptosis through the Mitochondria-Mediated Pathway.

J Nat Prod 2021 May 12. Epub 2021 May 12.

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, People's Republic of China.

1-Deoxynojirimycin, an α-glucosidase inhibitor, possesses various biological activities such as antitumor, antidiabetic, and antiviral effects. However, the application of 1-deoxynojirimycin is restricted by its poor lipophilicity and low bioavailability. In this study, three 1-deoxynojirimycin derivatives (-) comprising 1-deoxynojirimycin and kaempferol were designed and synthesized to modify their pharmacokinetics and improve their antitumor efficacy. Read More

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Natural Compound from Olive Oil Inhibits S100A9 Amyloid Formation and Cytotoxicity: Implications for Preventing Alzheimer's Disease.

ACS Chem Neurosci 2021 May 12. Epub 2021 May 12.

Department of Medical Biochemistry and Biophysics, Umeå University, 90187 Umeå, Sweden.

Polyphenolic compounds in the Mediterranean diet have received increasing attention due to their protective properties in amyloid neurodegenerative and many other diseases. Here, we have demonstrated for the first time that polyphenol oleuropein aglycone (OleA), which is the most abundant compound in olive oil, has multiple potencies for the inhibition of amyloid self-assembly of pro-inflammatory protein S100A9 and the mitigation of the damaging effect of its amyloids on neuroblastoma SH-SY5Y cells. OleA directly interacts with both native and fibrillar S100A9 as shown by intrinsic fluorescence and molecular dynamic simulation. Read More

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Effects of Trehalose on Lipid Membranes under Rapid Cooling using All-Atom and Coarse-Grained Molecular Simulations.

J Phys Chem B 2021 May 12. Epub 2021 May 12.

Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States.

We investigate the effect of the cryopreservative α-α-trehalose on a model 1,2-dimyristoyl--glycero-3-phosphocholine (DMPC) lipid membrane undergoing cooling from 350 to 250 K using all-atom (AA) and coarse-grained (CG) molecular dynamics simulation. In the AA simulations, we find that the addition of trehalose alters the (liquid crystalline) to (ripple) phase transition, suppressing the major domain of the phase and increasing the degree of leaflet interdigitation (the minor domain) which yields a thinner membrane with a higher area per lipid. Calculation of dihedral angle distributions for the lipid tails shows a greater fraction of angles in the phase as trehalose concentration is increased, indicating that trehalose increases lipid disorder in the membrane. Read More

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Multivalent Affinity Profiling: Direct Visualization of the Superselective Binding of Influenza Viruses.

ACS Nano 2021 May 12. Epub 2021 May 12.

Department of Molecules & Materials, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.

The influenza A virus (IAV) interacts with the glycocalyx of host cells through its surface proteins hemagglutinin (HA) and neuraminidase (NA). Quantitative biophysical measurements of these interactions may help to understand these interactions at the molecular level with the long-term aim to predict influenza infectivity and answer other biological questions. We developed a method, called multivalent affinity profiling (MAP), to measure virus binding profiles on receptor density gradients to determine the threshold receptor density, which is a quantitative measure of virus avidity toward a receptor. Read More

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Endocytosis induction by high-pulsed magnetic fields to overcome cell membrane barrier and improve chemotherapy efficiency.

Electromagn Biol Med 2021 May 12:1-8. Epub 2021 May 12.

Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Cell membrane acts as a barrier to the entry of impermeable drugs into cells. Recent studies have suggested that using magnetic fields can enable molecules to overcome the cell membrane barrier. However, the mechanism of membrane permeabilization remains unclear. Read More

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Osmotic tolerance of rabbit spermatozoa is affected by extender composition and temperature.

Anim Reprod Sci 2021 May 6;229:106763. Epub 2021 May 6.

Faculty of Biosciences and Technologies for Agriculture Food and Environment, University of Teramo, via Balzarini 1, Teramo, 64100, Italy. Electronic address:

Sperm osmotic adaptability to anisosmotic conditions is important for sperm epididymal maturation, motility activation at ejaculation, and female tract colonization, or for conducting technological procedures such as cryopreservation. Several factors affect this adaptability, including the fluid composition that contributes to water flow dynamics, and the temperature at which osmotic stress is initiated. This study was designed to investigate the effect of medium composition (electrolyte- or sugar-based extender) and temperature (25 and 5 °C) on rabbit sperm adaptability to anisosmotic conditions. Read More

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Unexpected acceleration of Ultrasonic-Assisted iodide dosimetry in the catalytic presence of ionic liquids.

Ultrason Sonochem 2021 Apr 25;74:105576. Epub 2021 Apr 25.

LRP UMR 5520, Université de Savoie Mont-Blanc, 73000 Chambéry, France.

This study investigates the potential of using small amounts of ionic liquids (IL) to enhance ultrasound-assisted extraction of lipids content from green microalgae. Three imidazolium-based ILs (butyl, octyl and dodecyl), each of them with two anions (bromide and acetate) were tested as additives. Viscosity and surface tension of the ILs aqueous mixtures were analyzed to determine the influence of ILs' anions and alkyl chain length, whereas KI dosimetry experiments were used as an indicator of radicals formation. Read More

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Gene transfer by electroporation with high frequency bipolar pulses in vitro.

Bioelectrochemistry 2021 Mar 22;140:107803. Epub 2021 Mar 22.

University of Ljubljana, Faculty of Electrical Engineering, Tržaška 25, 1000 Ljubljana, Slovenia. Electronic address:

High-frequency bipolar pulses (HF-BP) have been demonstrated to be efficient for membrane permeabilization and irreversible electroporation. Since membrane permeabilization has been achieved using HF-BP pulses we hypothesized that with these pulses we can also achieve successful gene electrotransfer (GET). Three variations of bursts of 2 µs bipolar pulses with 2 µs interphase delay were applied in HF-BP protocols. Read More

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