61,776 results match your criteria membrane damage

Host lactosylceramide enhances Edwardsiella tarda infection.

Cell Microbiol 2021 May 14:e13365. Epub 2021 May 14.

The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.

Edwardsiella tarda is a Gram-negative bacterium causing economic damage in the aquaculture. The interaction of E. tarda with microdomains is an important step in invasion, but the target molecules in microdomains remain undefined. Read More

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Incubation with Cholesterol-loaded Cyclodextrin and Subsequent Dilution in Partially Deoxygenated Extender Improves the Freezability of Cross-bred Bull Sperm.

Cryo Letters 2020 Sep-Oct;41(5):257-266

Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.

Background: The cryopreservation process induces osmotic stress, membrane changes and production of reactive oxygen species resulting in damage to the spermatozoa. Together, the presence of oxygen in the extender aggravates the oxidative stress that further reduces the cryosurvival rate of sperm cells.

Objective: To study the combined effect of cholesterol loaded cyclodextrin (CLC) and partial deoxygenation on post-thaw semen quality in crossbred bulls. Read More

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Elastic Photonic Microcapsules Containing Colloidal Crystallites as Building Blocks for Macroscopic Photonic Surfaces.

ACS Nano 2021 May 14. Epub 2021 May 14.

Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.

Colloidal crystals develop structural colors through wavelength-selective diffraction. Recently, a granular format of colloidal crystals has emerged as building blocks to construct macroscopic photonic surfaces or architectures with high reconfigurability through the secondary assembly. Here, we design elastic photonic microcapsules containing colloidal crystallites along the inner wall as a building block. Read More

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extract causes precocious acrosome reaction and viability loss but low rate of membrane damage in mouse spermatozoa.

J Anim Sci Technol 2021 Jan 31;63(1):58-68. Epub 2021 Jan 31.

Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu 44618, Nepal.

Several herbs including are known to possess conceptive property. In the present study, mouse spermatozoa were incubated with ethanol extract of leaves. The effect of extract on acrosome exocytosis was studied by labeling spermatozoa with fluorescein isothiocyanate (FITC) peanut agglutinin and by staining with Coomassie blue. Read More

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January 2021

Potential of watermelon () to maintain oxidative stability of rooster semen for artificial insemination.

J Anim Sci Technol 2021 Jan 31;63(1):46-57. Epub 2021 Jan 31.

Animal Science Department, Ahmadu Bello University, Zaria, Kaduna State 810241, Nigeria.

Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon () fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1-7. Read More

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January 2021

Capsaicin protects cardiomyocytes against lipopolysaccharide-induced damage via 14-3-3γ-mediated autophagy augmentation.

Front Pharmacol 2021 27;12:659015. Epub 2021 Apr 27.

Institute of Cardiovascular Diseases, Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China.

The myocardium is susceptible to lipopolysaccharide (LPS)-induced damage in sepsis, and cardiac dysfunction is a leading cause of mortality in patients with sepsis. The changes in cardiomyocyte autophagy in sepsis and the effects and mechanism of action of capsaicin (Cap) remain unclear. The potential pathway of 14-3-3γ-dependent autophagy and the effects and mechanisms of Cap were studied in LPS-induced injury to primary cultured neonatal rat cardiomyocytes. Read More

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Juggling with lipids, a game of Russian roulette.

Trends Endocrinol Metab 2021 May 10. Epub 2021 May 10.

Helmholtz Zentrum München, Institute of Metabolism and Cell Death, 85764 Neuherberg, Germany; Pirogov Russian National Research Medical University, Laboratory of Experimental Oncology, Moscow 117997, Russia. Electronic address:

Lipid peroxidation (LPO) is the molecular mechanism involved in oxidative damage of cellular membranes and the hallmark of a nonapoptotic form of cell death, known as ferroptosis. This iron-dependent cell death is an emerging strategy in cancer treatment and one of the central cell death mechanisms accounting for early cell loss and organ dysfunction in both neurodegenerative disease and ischemia-reperfusion injury. Although the biological roles of LPO products have attracted considerable attention, not only for their pathological mechanisms but also for their potential clinical application as biomarkers, the existence of a common lethal lipid death signal generated during ferroptosis remains poorly explored. Read More

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Removal of antibiotic-resistant genes during drinking water treatment: A review.

J Environ Sci (China) 2021 Jun 30;104:415-429. Epub 2020 Dec 30.

Zhejiang Key Laboratory of Drinking Water Safety and Distribution Technology, Zhejiang University, Hangzhou 310058, China; Ocean College, Zhejiang University, Hangzhou 310058, China. Electronic address:

Once contaminate the drinking water source, antibiotic resistance genes (ARGs) will propagate in drinking water systems and pose a serious risk to human health. Therefore, the drinking water treatment processes (DWTPs) are critical to manage the risks posed by ARGs. This study summarizes the prevalence of ARGs in raw water sources and treated drinking water worldwide. Read More

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Toxic response of the freshwater green algae Chlorella pyrenoidosa to combined effect of flotation reagent butyl xanthate and nickel.

Environ Pollut 2021 May 3;286:117285. Epub 2021 May 3.

School of Water Resources and Environment, Research Center of Environmental Science and Engineering, China University of Geosciences (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.

Butyl Xanthate (BX) is a typical flotation reagent used to extract non-ferrous nickel ores, discharged into the surrounding environment of mining areas in large quantities. However, few studies have focused on the toxicity of combined pollution of BX and nickel (Ni) on aquatic plants, especially phytoplankton, the main producer of aquatic ecosystems. The toxicity and potential mechanism of single and combined pollution of BX and Ni at different concentrations (0-20 mg L) on typical freshwater algae (Chlorella pyrenoidosa) were studied. Read More

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Mechanistic insight of simultaneous removal of tetracycline and its related antibiotic resistance bacteria and genes by ferrate(VI).

Sci Total Environ 2021 May 3;786:147492. Epub 2021 May 3.

College of the Environment and Ecology, Xiamen University, Xiamen 361102, China. Electronic address:

The emergence of antibiotics and their corresponding antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have posed great challenges to the public health. The paper demonstrates the removal of co-existing tetracycline (TC), its resistant Escherichia coli (E. coli), and ARGs (tetA and tetR) in a mixed system by applying ferrate(VI) (FeO, Fe(VI)) at pH 7. Read More

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Inhibition of human sperm motility and capacitation by ziram is mediated by decreasing tyrosine protein kinase.

Ecotoxicol Environ Saf 2021 May 10;218:112281. Epub 2021 May 10.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou, Zhejiang 325027, China. Electronic address:

Many endocrine disruptors may interfere with sperm motility, hyperactivation, and capacitation, thereby leading to male infertility. In the current study, we screened 14 endocrine disruptors, including plant ingredients, cigarette ingredients, minerals, insecticides and fungicides, plastics, and plasticizers, to inhibit human sperm motility and forward motility. Only ziram, a dithiocarbamate fungicide, can effectively inhibit sperm motility, forward motility, hyperactivation, capacitation, and spontaneous acrosome reaction of normal human spermatozoa. Read More

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Anabolic-androgenic steroids impair mitochondrial function and redox status in the heart and liver of mice.

Steroids 2021 May 10:108861. Epub 2021 May 10.

Laboratory of Neurotrauma and Biomarkers, PPG-Ciências Biológicas Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brasil. Electronic address:

Supraphysiological doses of anabolic-androgenic steroids (AAS) may cause long-term functional abnormalities, particularly in the heart and liver, which may only represent the later-stage of the cumulative damage caused by dysfunctional organelles. We investigated whether mid-term supraphysiological doses of Testosterone and Nandrolone impair mitochondrial Ca and membrane potential (ΔΨm) dynamics, and redox machinery in the heart and liver of mice. CF1 albino mice were treated daily with 15 mg/kg of Nandrolone (ND) or Testosterone (T), or oil (vehicle) for 19 days. Read More

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The peptidoglycan-associated protein NapA plays an important role in the envelope integrity and in the pathogenesis of the lyme disease spirochete.

PLoS Pathog 2021 May 13;17(5):e1009546. Epub 2021 May 13.

Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, United States of America.

The bacterial pathogen responsible for causing Lyme disease, Borrelia burgdorferi, is an atypical Gram-negative spirochete that is transmitted to humans via the bite of an infected Ixodes tick. In diderms, peptidoglycan (PG) is sandwiched between the inner and outer membrane of the cell envelope. In many other Gram-negative bacteria, PG is bound by protein(s), which provide both structural integrity and continuity between envelope layers. Read More

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Influences and mechanisms of nanoparticles on pentachloronitrobenzene accumulation by earthworms.

Environ Sci Pollut Res Int 2021 May 13. Epub 2021 May 13.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.

Pesticides and nanoparticles may coexist in soil; however, influences of nanoparticles on accumulation of pesticides in terrestrial organisms are still unclear. This study aims to investigate the influences and mechanisms of metal oxide nanoparticles (nano ZnO and nano CuO) on accumulation of pentachloronitrobenzene (PCNB) in earthworms and their combined toxicity. The earthworms were cultivated in the soil spiked with nanoparticles (10, 50, 250 mg/kg) and PCNB (100 μg/kg) for 21 days. Read More

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[Protective effects of Dendrobium officinale superfine powder against LPS-induced intestinal mucosal injury in mice].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(7):1667-1673

Zhejiang Chinese Medical University Hangzhou 310053, China.

This study aims to investigate the preventive effect of Dendrobium officinale in LPS-induced intestinal mucosal damage. Forty SPF-grade C57 BL/6 J male mice were randomly divided into normal group(NC), model group(LPS), and two superfine powder groups of Dendrobium officinale(DOF)(DOF-L, 0.30 g·kg~(-1)and DOF-H, 0. Read More

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Effects of Physical Agents on Muscle Healing with a Focus on Animal Model Research.

Miki Sakamoto

Phys Ther Res 2021 24;24(1):1-8. Epub 2021 Feb 24.

Department of Physical Therapy, School of Allied Health Sciences, Kitasato University, Japan.

Skeletal muscle injury is caused by a variety of events, such as muscle laceration, contusions, or strain. Muscle fibers respond to minor damage with immediate repair mechanisms that reseal the cell membrane. On the other hand, repair of irreversibly damaged fibers is achieved by activation of muscle precursor cells. Read More

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February 2021

Antioxidant, histopathological and biochemical outcomes of short-term exposure to acetamiprid in liver and brain of rat: The protective role of N-acetylcysteine and S-methylcysteine.

Saudi Pharm J 2021 Mar 21;29(3):280-289. Epub 2021 Feb 21.

Department of Molecular and Cell Biology, Faculty of Basic Science. University of Mazandaran, Babolsar, Iran.

The present study was conducted to investigate the protective effects of N-Acetyl-L-cysteine (NAC) and S-methyl- L-cysteine (SMC) against hepatic oxidative stress and brain damage induced by acetamiprid (ACP) in rats, which were evaluated by histopathological changes, measuring serum biomarkers and antioxidant defense systems. In this study, 42 rats were randomly divided into 6 groups and administered by intraperitoneally for one week: the control group, the sham group (normal saline), ACP alone (5 mg/kg) (group1), NAC alone (160 mg/kg) (group2), ACP + SMC (100 mg/kg) (group3), ACP + NAC (group 4) and ACP + NAC + SMC (group 5). Our results showed that acetamiprid induces liver injures including infiltration of inflammatory cells, congestion and altered histo-architecture and brain damages including gliosis, hyperemia and necrosis. Read More

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Effects of Silver Nanoparticles and Silver Nitrate on mRNA and microRNA Expression in Human Hepatocellular Carcinoma Cells (HepG2).

J Nanosci Nanotechnol 2021 Nov;21(11):5414-5428

US Environmental Protection Agency, Retired from EPA, Durham NC 27709, USA.

In order to understand toxicity of nano silver, human hepatocellular carcinoma (HepG2) cells were treated either with silver nitrate (AgNO₃) or with nano silver capped with glutathione (Ag-S) at various concentration. Differentially expressed genelists for mRNA and microRNA were obtained through Illumina RNA sequencing and DEseq data analyses. Both treatments showed non-linear dose response relationships for mRNA and microRNA. Read More

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November 2021

Determination of the LD with the chick embryo chorioallantoic membrane (CAM) assay as a promising alternative in nanotoxicological evaluation.

Nanotoxicology 2021 May 12:1-16. Epub 2021 May 12.

Department of Otorhinolaryngology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany.

Toxicity tests in rodents are still considered a controversial topic concerning their ethical justifiability. The chick embryo chorioallantoic membrane (CAM) assay may offer a simple and inexpensive alternative. The CAM assay is easy to perform and has low bureaucratic hurdles. Read More

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Crystalline ruthenium polypyridine nanoparticles: a targeted treatment of bacterial infection with multifunctional antibacterial, adhesion and surface-anchoring photosensitizer properties.

J Mater Chem B 2021 May;9(18):3808-3825

School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.

Photodynamic antibacterial therapy employs nanocomposites as an alternative to traditional antibiotics for the treatment of bacterial infections. However, many of these antibacterial materials are less effective towards bacteria than traditional drugs, either due to poor specificity or antibacterial activity. This can result in needless and excessive drug use in treatments. Read More

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Topological Reorganizations in the Rat Brain-Isolated Mitochondria after 72 Hours of Paradoxical Sleep Deprivation Revealed by Electron Cryo-Tomography.

Am J Physiol Cell Physiol 2021 May 12. Epub 2021 May 12.

Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, Chinagrid.43169.39.

Sleep deprivation has profound influence on several aspects of health and disease. Mitochondria dysfunction has been implicated to play an essential role in the neuronal cellular damage induced by sleep deprivation, but little is known about how neuronal mitochondrial ultrastructure is affected under sleep deprivation. In this report, we utilized electron cryo-tomography to reconstruct the three-dimensional (3D) mitochondrial structure and extracted morphometric parameters to quantitatively characterize its reorganizations. Read More

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Ferroptosis in infection, inflammation, and immunity.

J Exp Med 2021 Jun 12;218(6). Epub 2021 May 12.

Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, The Third Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.

Ferroptosis is a type of regulated necrosis that is triggered by a combination of iron toxicity, lipid peroxidation, and plasma membrane damage. The upstream inducers of ferroptosis can be divided into two categories (biological versus chemical) and activate two major pathways (the extrinsic/transporter versus the intrinsic/enzymatic pathways). Excessive or deficient ferroptotic cell death is implicated in a growing list of physiological and pathophysiological processes, coupled to a dysregulated immune response. Read More

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Identification of Key Genes and Pathways Associated with Hepatosplenic T-Cell Lymphoma (HSTCL) by Bioinformatics Analysis.

Clin Lab 2021 May;67(5)

Background: Prognosis of Hepatosplenic T-Cell Lymphoma (HSTCL) is very poor, while the molecular mechanism of this disease has rarely been investigated and remains mysterious. The aim of the study is to screen differentially expressed genes (DEGs) of patients with HSTCL and normal controls, explore the pathogenesis, and provide guidance for the gene diagnosis and precise treatment of HSTCL.

Methods: The genetic chip data GSE57520 of HSTCL was searched from the GEO database, and the quality control and DEGs screening were performed through BART online tools. Read More

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Efficient Killing of Multidrug-Resistant Internalized Bacteria by AIEgens In Vivo.

Adv Sci (Weinh) 2021 05 2;8(9):2001750. Epub 2021 Mar 2.

Center for AIE Research College of Materials Science and Engineering Shenzhen University Shenzhen 518061 China.

Bacteria infected cells acting as "Trojan horses" not only protect bacteria from antibiotic therapies and immune clearance, but also increase the dissemination of pathogens from the initial sites of infection. Antibiotics are hard and insufficient to treat such hidden internalized bacteria, especially multidrug-resistant (MDR) bacteria. Herein, aggregation-induced emission luminogens (AIEgens) such as ,-diphenyl-4-(7-(pyridin-4-yl) benzo [] [1,2,5] thiadiazol-4-yl) aniline functionalized with 1-bromoethane (TBP-1) and (3-bromopropyl) trimethylammonium bromide (TBP-2) (TBPs) show potent broad-spectrum bactericidal activity against both extracellular and internalized Gram-positive pathogens. Read More

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Resveratrol against 6-OHDA-induced damage of PC12 cells via PI3K/Akt.

Transl Neurosci 2021 Jan 19;12(1):138-144. Epub 2021 Apr 19.

Key Laboratory of Basic Pharmacology and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, No. 6, Xuefu West Road, Xinpu New District, Zunyi 563000, Guizhou, China.

Objective: Our previous study found that resveratrol (Res), which is a phytoalexin, attenuated 6-hydroxydopamine (6-OHDA)-induced motor dysfunction by activating the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway in rats. Therefore, we further explored the protective effect of Res on 6-OHDA-induced damage to PC12 cells with respect to the PI3K/Akt signaling pathway.

Methods: We incubated PC12 cells with 75 μM 6-OHDA for 24 h, and Res was then added at a final concentration of 25 μM; the protective effect was examined via MTT and lactate dehydrogenase (LDH) assays. Read More

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January 2021

Bilateral Traumatic Maculopathy after Closed-Globe Injury in Context of National Lockdown: A Case Report.

Case Rep Ophthalmol 2021 Jan-Apr;12(1):159-163. Epub 2021 Apr 12.

Department of Ophthalmology 2, Quinze-Vingts National Ophthalmology Hospital, IHU FOReSIGHT, University Paris Descartes, Paris, France.

The aim of this study is to report a case of bilateral traumatic maculopathy in a 19-year-old male following blunt trauma during 2020 coronavirus disease-2019 national lockdown. Descriptive case report based on data from clinical records, patient observation, and analysis of diagnostic tests. A previously healthy, 19-year-old male presented for a visit in our department at 15-20 National Ophthalmology Institute with complaints of bilateral decreased visual acuity. Read More

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A novel thermostable beetle luciferase based cytotoxicity assay.

Sci Rep 2021 May 11;11(1):10002. Epub 2021 May 11.

Jane Anne Nohl Division of Hematology and Center for the Study of Blood Diseases, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Cytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the development of a new cytotoxicity assay termed 'Matador-Glo assay' which takes advantage of a thermostable variant of Click Beetle Luciferase (Luc146-1H2). Read More

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Proteomic and Transcriptomic Analysis of φX174 Infection Reveals Broad Upregulation of Host Escherichia coli Membrane Damage and Heat Shock Responses.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia

Measuring host-bacteriophage dynamics is an important approach to understanding bacterial survival functions and responses to infection. The model bacteriophage φX174 is endemic to the human gut and has been studied for over 70 years, but the host response to infection has never been investigated in detail. To address this gap in our understanding of this important interaction within our microbiome, we have measured host C proteomic and transcriptomic response to φX174 infection. Read More

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Selenium nanoparticles ameliorate Brassica napus L. cadmium toxicity by inhibiting the respiratory burst and scavenging reactive oxygen species.

J Hazard Mater 2021 Apr 16;417:125900. Epub 2021 Apr 16.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China. Electronic address:

Cadmium (Cd) is a widely distributed soil contaminant which induces oxidative damage and is therefore toxic to plants. Although selenium oxyanions such as selenite (SeO) and selenate (SeO) can alleviate Cd stress to plants, it is not known whether selenium nanoparticles (SeNPs) are able to do the same. The present study demonstrated the positive impact of both SeNPs and SeO on Brassica napus L. Read More

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Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia.

Cell 2021 Apr 24. Epub 2021 Apr 24.

QBI COVID-19 Research Group (QCRG), San Francisco, CA, USA; Department of Pharmaceutical Chemistry, University of California, San Francisco (UCSF), San Francisco, CA, USA. Electronic address:

Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is initiated by binding of the viral Spike protein to host receptor angiotensin-converting enzyme 2 (ACE2), followed by fusion of viral and host membranes. Although antibodies that block this interaction are in emergency use as early coronavirus disease 2019 (COVID-19) therapies, the precise determinants of neutralization potency remain unknown. We discovered a series of antibodies that potently block ACE2 binding but exhibit divergent neutralization efficacy against the live virus. Read More

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