73,392 results match your criteria membrane components


Electrochemical sensor for tricyclic antidepressants with low nanomolar detection limit: Quantitative Determination of Amitriptyline and Nortriptyline in blood.

Talanta 2021 Nov 15;239:123072. Epub 2021 Nov 15.

Department of Ophthalmology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37232, USA.

Amitriptyline and its metabolite, Nortriptyline are commonly used tricyclic antidepressant (TCA) drugs that are electrochemically active. In this work, the performance characteristics of a plasticized PVC membrane-coated glassy carbon (GC) electrode are described for the voltammetric quantification of Amitriptyline and Nortriptyline in whole blood. The highly lipophilic Amitriptyline and Nortriptyline preferentially partition into the plasticized PVC membrane where the free drug is oxidized on the GC electrode. Read More

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November 2021

Effect of the cholesterol on electroporation of planar lipid bilayer.

Bioelectrochemistry 2021 Nov 23;144:108004. Epub 2021 Nov 23.

University of Ljubljana, Faculty of Electrical Engineering, Slovenia.

Electroporation threshold depends on the membrane composition, with cholesterol being one of its key components already studied in the past, but the results were inconclusive. The aim of our study was to determine behaviour of planar lipid bilayers with varying cholesterol concentrations under electric field. This would give us a better insight into cholesterol effect on membrane properties during electroporation process, since cholesterol is one of the major components of biological membranes and plays a crucial role in membrane organisation, dynamics, and function. Read More

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November 2021

Long-term, synergistic and high-efficient antibacterial polyacrylonitrile nanofibrous membrane prepared by "one-pot" electrospinning process.

J Colloid Interface Sci 2021 Nov 18. Epub 2021 Nov 18.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China; College of Chemical Engineering, Sichuan University, Chengdu 610065, China. Electronic address:

Enhancing long-term antibacterial activity of membrane materials is an effective strategy to reduce biological contamination. Herein, we developed a long-term, synergistic antibacterial polyacrylonitrile (PAN) nanofiber membrane by a "one-pot" electrospinning process. In the reaction solution of PAN and N, N-dimethylformamide (DMF), silver-silicon dioxide nanoparticles ([email protected] NPs) are in-situ synthesized and stabilized using silane coupling agent; and [2-(methacryloyloxy)-ethyl] trimethylammonium chloride (MT) monomers are then in-situ cross-linked to obtain a polyquaternary ammonium salt (PMT). Read More

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November 2021

MSCs and their exosomes: a rapidly evolving approach in the context of cutaneous wounds therapy.

Stem Cell Res Ther 2021 Dec 4;12(1):597. Epub 2021 Dec 4.

Stem Cells and Regenerative Medicine Unit at King Fahad Medical Research Centre, Jeddah, Saudi Arabia.

Currently, mesenchymal stem/stromal stem cell (MSC) therapy has become a promising option for accelerating cutaneous wound healing. In vivo reports have outlined the robust competences of MSCs to offer a solid milieu by inhibition of inflammatory reactions, which in turn, enables skin regeneration. Further, due to their great potential to stimulate angiogenesis and also facilitate matrix remodeling, MSCs hold substantial potential as future therapeutic strategies in this context. Read More

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December 2021

Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET.

Curr Top Membr 2021 16;88:75-118. Epub 2021 Nov 16.

Department of Physics, University of York, York, United Kingdom; Department of Biology, University of York, York, United Kingdom. Electronic address:

Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these events are coordinated and how they integrate with regulation of molecular crowding. We use the budding yeast Saccharomyces cerevisiae as a model system to study these questions using recent progress in optical fluorescence microscopy and crowding sensing probe technology. Read More

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November 2021

Membrane tension.

Curr Top Membr 2021 2;88:189-203. Epub 2021 Nov 2.

Department of Physiology and Biophysics, University at Buffalo, The State University of New York, Buffalo, NY, United States. Electronic address:

The cell membrane serves as a barrier that restricts the rate of exchange of diffusible molecules. Tension in the membrane regulates many crucial cell functions involving shape changes and motility, cell signaling, endocytosis, and mechanosensation. Tension reflects the forces contributed by the lipid bilayer, the cytoskeleton, and the extracellular matrix. Read More

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November 2021

CRMP4-mediated fornix development involves semaphorin-3E signaling pathway.

Elife 2021 Dec 3;10. Epub 2021 Dec 3.

Inserm, U1216, CEA, Grenoble Institut Neurosciences, University Grenoble Alpes, Grenoble, France.

Neurodevelopmental axonal pathfinding plays a central role in correct brain wiring and subsequent cognitive abilities. Within the growth cone, various intracellular effectors transduce axonal guidance signals by remodeling the cytoskeleton. Semaphorin-3E (Sema3E) is a guidance cue implicated in development of the fornix, a neuronal tract connecting the hippocampus to the hypothalamus. Read More

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December 2021

Gene Expression in Amnion-Derived Cells Cultured on Recombinant Laminin 332-A Preliminary Study.

Front Med (Lausanne) 2021 10;8:719899. Epub 2021 Nov 10.

Department of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland.

Human amniotic cells (hAC) exhibit characteristics of undifferentiated cells and immunomodulatory properties. Recognition of the relationship between amniotic cells and components of the extracellular matrix is an important condition for their preparation and further successful clinical application in regenerative medicine and transplantology. Laminin 332 (LN-332), as a natural component of the basement membrane of amniotic epithelial cells and a ligand for integrin receptors, may strongly influence the phenotype and fate of amniotic cells. Read More

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November 2021

Fighting salt or enemies: shared perception and signaling strategies.

Curr Opin Plant Biol 2021 Nov 29;64:102120. Epub 2021 Nov 29.

Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, 6708 PB, the Netherlands. Electronic address:

Plants react to a myriad of biotic and abiotic environmental signals through specific cellular mechanisms required for survival under stress. Although pathogen perception has been widely studied and characterized, salt stress perception and signaling remain largely elusive. Recent observations, obtained in the model plant Arabidopsis thaliana, show that perception of specific features of pathogens also allows plants to mount salt stress resilience pathways, highlighting the possibility that salt sensing and pathogen perception mechanisms partially overlap. Read More

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November 2021

Deoxysphingolipids Upregulate MMP-1, Downregulate TIMP-1, and Induce Cytotoxicity in Human Schwann Cells.

Neuromolecular Med 2021 Dec 1. Epub 2021 Dec 1.

Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC, 29401, USA.

Sphingolipids are a heterogeneous class of lipids and essential components of the plasma membrane and plasma lipoproteins. Studies have shown that plasma deoxysphingolipid (DSL), a newly identified sphingolipid class, is increased in diabetic patients and associated with diabetic neuropathy. However, it remains unknown if there is a causal relationship between plasma DSL increase and diabetic neuropathy. Read More

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December 2021

Decreased dopaminergic inhibition of pyramidal neurons in anterior cingulate cortex maintains chronic neuropathic pain.

Cell Rep 2021 Nov;37(9):109933

Montréal Neurological Institute, Department of Neurology & Neurosurgery, Alan Edwards Centre for Research on Pain, McGill University, Montréal, QC H3A 2B4, Canada. Electronic address:

Pyramidal neurons in the anterior cingulate cortex (ACC), a prefrontal region involved in processing the affective components of pain, display hyperexcitability in chronic neuropathic pain conditions, and their silencing abolishes hyperalgesia. We show that dopamine, through D1 receptor (D1R) signaling, inhibits pyramidal neurons of mouse ACC by modulation of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Activation of G-coupled D1R by dopamine induces the opening of HCN channels at physiological membrane potentials, driving a significant decrease in input resistance and excitability. Read More

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November 2021

Chemistry of Lipid Nanoparticles for RNA Delivery.

Acc Chem Res 2021 Dec 1. Epub 2021 Dec 1.

Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Robertson Life Science Building, 2730 South Moody Avenue, Portland, Oregon 97201, United States.

ConspectusLipid nanoparticles (LNPs) are a type of lipid vesicles that possess a homogeneous lipid core. These vesicles are widely used in small-molecule drug and nucleic acid delivery and recently gained much attention because of their remarkable success as a delivery platform for COVID-19 mRNA vaccines. Nonetheless, the utility of transient protein expression induced by mRNA extends far beyond vaccines against infectious diseases─they also hold promise as cancer vaccines, protein replacement therapies, and gene editing components for rare genetic diseases. Read More

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December 2021

An extracellular matrix damage sensor signals through membrane-associated kinase DRL-1 to mediate cytoprotective responses in Caenorhabditis elegans.

Genetics 2021 Nov 27. Epub 2021 Nov 27.

Department of Biology and Genetics Institute, University of Florida, Gainesville, FL 32611, USA.

We and others previously identified circumferential bands of collagen named annular furrows as key components of a damage sensor in the cuticle of Caenorhabditis elegans that regulates cytoprotective genes. Mutation or loss of non-collagen secreted proteins OSM-7, OSM-8, and OSM-11 activate the same cytoprotective responses without obvious changes to the cuticle indicating that other extracellular proteins are involved. Here, we used RNAi screening to identify protein kinase DRL-1 as a key modulator of cytoprotective gene expression and stress resistance in furrow and extracellular OSM protein mutants. Read More

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November 2021

A distinct inner nuclear membrane proteome in Saccharomyces cerevisiae gametes.

G3 (Bethesda) 2021 Oct 16. Epub 2021 Oct 16.

Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

The inner nuclear membrane (INM) proteome regulates gene expression, chromatin organization, and nuclear transport; however, it is poorly understood how changes in INM protein composition contribute to developmentally regulated processes, such as gametogenesis. We conducted a screen to determine how the INM proteome differs between mitotic cells and gametes. In addition, we used a strategy that allowed us to determine if spores synthesize their INM proteins de novo, rather than inheriting their INM proteins from the parental cell. Read More

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October 2021

Interactions between a mechanosensitive channel and cell wall integrity signaling influence pollen germination in Arabidopsis thaliana.

J Exp Bot 2021 Nov 29. Epub 2021 Nov 29.

Department of Biology, Washington University in St. Louis, St. Louis, MO.

Cells employ multiple systems to maintain cellular integrity, including mechanosensitive (MS) ion channels and the cell wall integrity (CWI) pathway. Here, we use pollen as a model system to ask how these different mechanisms are interconnected at the cellular level. MscS-Like (MSL)8 is an MS channel required to protect Arabidopsis thaliana pollen from osmotic challenges during in vitro rehydration, germination and tube growth. Read More

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November 2021

Crystal structures of phosphatidyl serine synthase PSS reveal the catalytic mechanism of CDP-DAG alcohol O-phosphatidyl transferases.

Nat Commun 2021 Nov 30;12(1):6982. Epub 2021 Nov 30.

Department of Structural Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, 60438, Frankfurt am Main, Germany.

Phospholipids are the major components of the membrane in all type of cells and organelles. They also are critical for cell metabolism, signal transduction, the immune system and other critical cell functions. The biosynthesis of phospholipids is a complex multi-step process with high-energy intermediates. Read More

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November 2021

BAT1 Amino Acid Transporter Complexed With SARS-CoV-2 Receptor ACE2 Forms a Heterodimer Functional Unit: Conformation Using Radiation Inactivation Analysis.

Function (Oxf) 2021 13;2(4):zqab027. Epub 2021 May 13.

School of Biological Sciences, Illinois State University, Normal, IL, 61790, USA.

The SARS-CoV-2 receptor, angiotensin-converting enzyme-2 (ACE2), is expressed at levels of greatest magnitude in the small intestine as compared with all other human tissues. Enterocyte ACE2 is coexpressed as the apical membrane trafficking partner obligatory for expression and activity of the BAT1 sodium-dependent neutral amino acid transporter. These components are assembled as an [ACE2:BAT1] dimer-of-heterodimers quaternary complex that putatively steers SARS-CoV-2 tropism in the gastrointestinal (GI) tract. Read More

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Pharmacokinetics of Commonly Used Medications in Children Receiving Continuous Renal Replacement Therapy: A Systematic Review of Current Literature.

Clin Pharmacokinet 2021 Nov 30. Epub 2021 Nov 30.

School of Pharmacy, University of Waterloo, 10 Victoria St S. Kitchener, Waterloo, ON, N2G 1C5, Canada.

Background And Objective: The use of continuous renal replacement therapy (CRRT) for renal support has increased substantially in critically ill children compared with intermittent modalities owing to its preferential effects on hemodynamic stability. With the expanding role of CRRT, the quantification of extracorporeal clearance and the effect on primary pharmacokinetic parameters is of the utmost importance. Within this review, we aimed to summarize the current state of the literature and compare published pharmacokinetic analyses of commonly used medications in children receiving CRRT to those who are not. Read More

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November 2021