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Reconfigurable Chirality of DNA-Bridged Nanorod Dimers.

ACS Nano 2021 Jul 22. Epub 2021 Jul 22.

Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, United States.

Gold nanorods assembled in a side-by-side chiral configuration have potential applications in sensing due to their strong chiroptical surface plasmon resonances. Recent experiments have shown that dimers of gold nanorods bridged by double-stranded DNA exhibit variable chiral configurations depending on the chemical and ionic properties of the solvent medium. Here, we uncover the underlying physics governing this intriguing chiral behavior of such DNA-bridged nanorods by theoretically evaluating their configurational free energy landscape. Read More

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Spatial and Temporal Variations in Pigment and Species Compositions of Snow Algae on Mt. Tateyama in Toyama Prefecture, Japan.

Front Plant Sci 2021 5;12:689119. Epub 2021 Jul 5.

Graduate School of Science, Chiba University, Chiba, Japan.

Snow algae are photosynthetic microbes that inhabit the melting snow surface in alpine and polar regions. We analyzed the pigment and species composition of colored snow collected on Mt. Tateyama in Japan during the melting seasons of 2015 and 2016. Read More

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Biosynthesis of Lactobionic Acid in Whey-Containing Medium by Microencapsulated and Free Bacteria of .

Kamila Goderska

Indian J Microbiol 2021 Sep 11;61(3):315-323. Epub 2021 May 11.

Department of Food Technology of Plant Origin, Department of Fermentation and Biosynthesis, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland.

The aim of this research was to develop a method of its production from whey using bacteria of the species . Analyses of the lactobionic acid production method from whey showed that the following factors have a significant effect on its efficiency: the frequency of whey batch feeding, pH and the type of bacteria application, i.e. Read More

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September 2021

Characterization of a traditional ripened cheese, Kurdish Kope: Lipolysis, lactate metabolism, the release profile of volatile compounds, and correlations with sensory characteristics.

J Food Sci 2021 Jul 19. Epub 2021 Jul 19.

Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Kope cheese has been characterized based on gross chemical composition, free fatty acids (FFAs), organic acids (OAs), volatile compounds (VCs), and sensory attributes (SAs) during 187 days of the ripening period. C16:0, C18:1cisΔ9, and C14:0 were the most abundant FFAs. Lactic and acetic acids were the most prevalent OAs affecting sensory properties. Read More

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SwabExpress: An end-to-end protocol for extraction-free covid-19 testing.

Clin Chem 2021 Jul 21. Epub 2021 Jul 21.

Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Background: The urgent need for massively scaled clinical testing for SARS-CoV-2, along with global shortages of critical reagents and supplies, has necessitated development of streamlined laboratory testing protocols. Conventional nucleic acid testing for SARS-CoV-2 involves collection of a clinical specimen with a nasopharyngeal swab in transport medium, nucleic acid extraction, and quantitative reverse transcription PCR (RT-qPCR) (1). As testing has scaled across the world, the global supply chain has buckled, rendering testing reagents and materials scarce (2). Read More

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Acceleration of DNA Hybridization Chain Reactions on 3D Nanointerfaces of Magnetic Particles and Their Direct Application in the Enzyme-Free Amplified Detection of microRNA.

ACS Appl Mater Interfaces 2021 Jul 21. Epub 2021 Jul 21.

Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8573, Japan.

Accelerated DNA hybridization chain reactions (HCRs) using DNA origami as a scaffold have received considerable attention in dynamic DNA nanotechnology. However, tailor-made designs are essential for DNA origami scaffolds, hampering the practical application of accelerated HCRs. Here, we constructed the semilocalized HCR and localized HCR systems using magnetic beads (MBs) as a simple scaffold to explore them for the enzyme-free miR-21 detection. Read More

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Application of the inverted classroom model in the teaching module "new classification of periodontal and peri-implant diseases and conditions" during the COVID-19 pandemic.

GMS J Med Educ 2021;38(5):Doc89. Epub 2021 Jun 15.

Hannover Medical School, Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover, Germany.

Due to the need for patient-free dental education during the COVID-19 pandemic, Hannover Medical School (MHH) implemented a new periodontology module. Its didactic structure was based on the "inverted classroom model" (ICM) in combination with elements of case-based learning. The educational objective was to increase the diagnostic confidence of dental students in the classification of periodontal patients (staging & grading), based on 33 digitized patient cases. Read More

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Cell-Free Biomimetic Osteochondral Scaffold for the Treatment of Knee Lesions: Clinical and Imaging Results at 10-Year Follow-up.

Am J Sports Med 2021 Jul 20:3635465211029292. Epub 2021 Jul 20.

SC II Clinica Ortopedica, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.

Background: Cell-free devices have been introduced to restore osteochondral defects, avoiding the limitations of cell-based procedures. Among these, an osteochondral scaffold made of type I collagen and hydroxyapatite has been investigated with promising results up to medium-term follow-up. However, the clinical and imaging results over time still need to be documented. Read More

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Mechanistic Insight into the O Evolution Catalyzed by Copper Complexes with Tetra- and Pentadentate Ligands.

J Phys Chem A 2021 Jul 20. Epub 2021 Jul 20.

Department of Chemistry, Indian Institute of Technology Hyderabad, Sangareddy 502285, Telangana, India.

The mononuclear complexes ([(bztpen)Cu] (BF) (bztpen = -benzyl-,','-tris (pyridin-2-yl methyl ethylenediamine))) and ([(dbzbpen)Cu(OH)] (BF) (dbzbpen = ,'-dibenzyl-,'-bis(pyridin-2-ylmethyl) ethylenediamine)) have been reported as water oxidation catalysts in basic medium (pH = 11.5). We explore the O evolution process catalyzed by these copper catalysts with various ligands (L) by applying the first-principles molecular dynamics simulations. Read More

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Study of a Current and Voltage Polarization Sensor Network.

Sensors (Basel) 2021 Jul 1;21(13). Epub 2021 Jul 1.

CPQD-Research and Development Center in Telecommunications, Campinas 13086-902, Brazil.

Sensors based on polarization are suitable for application in power grids due to their excellent characteristics, such as high electrical insulation, non-magnetic saturation, oil-free, no risk of explosive failures, and high bandwidth. Utility companies are incorporating new technologies that are driving the evolution of electrical systems. Thus, it is interesting to evaluate the possibility of using polarization sensors in a network configuration. Read More

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Elevated S-adenosylhomocysteine induces adipocyte dysfunction to promote alcohol-associated liver steatosis.

Sci Rep 2021 Jul 19;11(1):14693. Epub 2021 Jul 19.

Research Service (151), Veterans Affairs Nebraska-Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE, 68105, USA.

It has been previously shown that chronic ethanol administration-induced increase in adipose tissue lipolysis and reduction in the secretion of protective adipokines collectively contribute to alcohol-associated liver disease (ALD) pathogenesis. Further studies have revealed that increased adipose S-adenosylhomocysteine (SAH) levels generate methylation defects that promote lipolysis. Here, we hypothesized that increased intracellular SAH alone causes additional related pathological changes in adipose tissue as seen with alcohol administration. Read More

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Claudin-7 deficiency promotes stemness properties in colorectal cancer through Sox9-mediated Wnt/β-catenin signalling.

J Transl Med 2021 Jul 19;19(1):311. Epub 2021 Jul 19.

Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background: Colorectal cancer (CRC) is a common malignant tumour of the digestive tract that is characterized by high patient morbidity and mortality rates. Claudin-7 (Cldn7), a tight junction protein, was recently reported to function as a candidate tumour suppressor gene in CRC. Our previous study demonstrated that the large intestine of C57/BL6 mice showed intestinal adenomas and abnormal Ki67 expression and distribution in the intestinal crypt when Cldn7 was knocked out. Read More

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Febuxostat reduces muscle wasting in tumor-bearing mice with LM8 osteosarcoma cells via inhibition of reactive oxygen species generation.

Free Radic Res 2021 Jul 19:1-11. Epub 2021 Jul 19.

Department of Orthopaedic Surgery, Mie university Graduate School of Medicine, Tsu, Japan.

Cachexic condition due to malignant tumors has been a challenging problem. The aim of this study is to analyze effects of febuxostat on both and models of the wasting of skeletal muscles, due to LM8 osteosarcoma cells. C2C12 myotubes were incubated in the conditioned medium of LM8. Read More

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Novel Cell Culture Paradigm Prolongs Mouse Corneal Epithelial Cell Proliferative Activity and .

Front Cell Dev Biol 2021 30;9:675998. Epub 2021 Jun 30.

Eye Institute & Affiliated Xiamen Eye Center, School of Pharmaceutical Sciences, School of Medicine, Xiamen University, Xiamen, China.

It has been a long-standing challenge to obtain from cell cultures adequate amounts of mouse corneal epithelial cells (mCEC) to perform transplantation surgery. This limitation is attributable to the passage dependent declines in their proliferative activity. We describe here development of a novel 6C medium that contains six different modulators of different signaling pathways, which control proliferative mCEC activity. Read More

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Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant.

Front Cell Dev Biol 2021 2;9:672862. Epub 2021 Jul 2.

Department of Obstetrics and Gynaecology, Medical Faculty, Cologne University, Cologne, Germany.

Mitochondria-targeted antioxidants have great potential to counterbalance the generated reactive oxygen species (ROS) because they cross the inner membrane of the mitochondria. Still, their use was not reported in vitrified human spermatozoa. Our laboratory has successfully vitrified spermatozoa without the use of permeable cryoprotectants, but subcellular-level evidence was missing. Read More

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Identification of Flap Endonuclease 1 With Diagnostic and Prognostic Value in Breast Cancer.

Front Oncol 2021 30;11:603114. Epub 2021 Jun 30.

Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, China.

Objective: This study aims to identify the potential value of flap endonuclease 1 (FEN1) as a diagnostic and prognostic marker for breast cancer (BC).

Methods: ELISA was used to measure serum FEN1 levels and ECLIA for CA153 and CEA levels. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value. Read More

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Medium long-term follow-up outcomes of BalMedic bovine pericardial bioprosthetic valve in the mitral position.

J Thorac Dis 2021 Jun;13(6):3652-3659

Department of Cardiac Surgery, The First People's Hospital of Yulin, Yulin, China.

Background: In China, where Rheumatic heart disease is still prevalent, two thirds of valve replacements are in mitral position. Before a valve replacement operation is conducted, most patients have reached Class III/IV heart function under the New York Heart Association (NYHA) classification system. As the background to this disease is quite different to that of degenerated cases, which are more prevalent in Western countries, the development of an artificial heart valve that is suitable for local patients is very significant for China. Read More

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Internet-delivered cognitive-behaviour therapy (ICBT) for obsessive-compulsive disorder when delivered as routine clinical care: A phase IV clinical trial.

J Anxiety Disord 2021 Jul 9;82:102444. Epub 2021 Jul 9.

MindSpot, Macquarie University, Sydney, NSW, Australia; eCentreClinic, Department of Psychology, Macquarie University, Sydney, NSW, Australia.

Cognitive-behaviour therapy (CBT) is an effective treatment for obsessive-compulsive disorder (OCD), but many patients experience difficulty accessing this treatment. Internet-delivered CBT (ICBT) enhances access to CBT for individuals with OCD and has been shown to be efficacious in Phase I, II, and III clinical trials. However, there are fewer studies investigating ICBT for OCD in Phase IV clinical trials, which demonstrate the effectiveness of the intervention when provided as part of routine care. Read More

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Trajectories of cardiovascular disease risk and their association with the incidence of cardiovascular events over 18 years of follow-up: The Tehran Lipid and Glucose study.

J Transl Med 2021 Jul 16;19(1):309. Epub 2021 Jul 16.

Department of Epidemiology and Biostatistics, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Understanding long-term patterns (trajectories) of cardiovascular diseases (CVD) risk and identifying different sub-groups with the same underlying risk patterns could help facilitate targeted cardiovascular prevention programs.

Methods: A total of 3699 participants of the Tehran Lipid and Glucose Study (TLGS) (43% men, mean age = 53.2 years), free of CVD at baseline in 1999-2001 and attending at least one re-examination cycle between the second (2002-2005) and fourth cycles (2009-2011) were included. Read More

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LncRNA KLK8 modulates stem cell characteristics in colon cancer.

Pathol Res Pract 2021 Apr 8;224:153437. Epub 2021 Apr 8.

Department of Digestive Endoscopy, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Background: Colon cancer, one of the most common and aggressive human malignancies, is the third leading cause of cancer-related death worldwide. Despite advances in systemic therapy, the 5-year survival rate of colon cancer patients remains at 30 % due to recurrence and metastasis. The poor prognosis is related to the presence of cancer stem cells (CSCs), and long non-coding RNAs (lncRNAs) play a significant role in CSCs. Read More

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Isolation and long-term expansion of murine epidermal stem-like cells.

PLoS One 2021 16;16(7):e0254731. Epub 2021 Jul 16.

Department of Environmental and Public Health Sciences, College of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of America.

Epidermis is the most outer layer of the skin and a physical barrier protecting the internal tissues from mechanical and environmental insults. The basal keratinocytes, which, through proliferation and differentiation, supply diverse cell types for epidermal homeostasis and injury repair. Sustainable culture of murine keratinocyte, however, is a major obstacle. Read More

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Immobilized lipase-catalyzed transesterification for synthesis of biolubricant from palm oil methyl ester and trimethylolpropane.

Bioprocess Biosyst Eng 2021 Jul 16. Epub 2021 Jul 16.

Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

The present study reports the effects of three commercial immobilized lipases namely Novozyme 435 from Candida antarctica lipase B (CALB), Lipozyme TL IM from Thermomyces lanuginosus and Lipozyme RM IM from Rhizomucor miehei on the production of trimethylolpropane (TMP) ester from high oleic palm methyl ester (HO-PME) and TMP. The TMP ester is a promising base oil for biolubricants that are easily biodegradable and non-toxic to humans and the environment. Enzymatic catalysts are insensitive to free fatty acid (FFA) content, hence able to mitigate the side reactions and consequently reduce product separation cost. Read More

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Peripheral Neurectomy for Trigeminal Neuralgia: A Report of Seventeen Cases and Review of the Literature.

Asian J Neurosurg 2021 Apr-Jun;16(2):281-287. Epub 2021 May 28.

Department of Neurosurgery, Armed Forces Hospital Southern Region, Khamis Mushait, Saudi Arabia.

Background: Surgical intervention for trigeminal neuralgia (TN) is indicated if there is a failure of the medical treatment. Peripheral neurectomy is one of the oldest surgical procedures for TN.

Objective: The aim is to evaluate the clinical outcome and the recurrence rate following peripheral neurectomy for the management of TN. Read More

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Establishment and Dissemination of Radiological Standards in the Field of Diagnostic Radiology in India.

J Med Phys 2021 Jan-Mar;46(1):33-40. Epub 2021 May 5.

Radiation Safety Systems Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.

Background: The increased use of ionizing radiation for diagnostic purpose has resulted in an increase in the world population dose. Patient dosimetry in X-ray diagnostic radiology is required to establish diagnostic reference levels (DRLs) and to assess the average dose received by organs and tissues. International bodies have recommended DRLs to be based on dosimetric quantities. Read More

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The Role Silver Nanoparticles Plays in Silver-Based Double-Perovskite Nanocrystals.

Chem Mater 2021 Apr 17;33(7):2370-2377. Epub 2021 Mar 17.

Department of Materials Science and Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.

Lead-free double perovskites are studied as an optional replacement to lead halide perovskites in optoelectronic applications. Recently, double-perovskite materials in which two divalent lead cations are replaced with an Ag and a trivalent cation have been demonstrated. The presence of a reactive silver cation and observations of metallic silver nanodecorations raised concerns regarding the stability and applicability of these materials. Read More

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Essential roles of plexin-B3 oligodendrocyte precursor cells in the pathogenesis of Alzheimer's disease.

Commun Biol 2021 Jul 15;4(1):870. Epub 2021 Jul 15.

Affective Disorders Research Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, Japan.

The role of oligodendrocyte lineage cells, the largest glial population in the adult central nervous system (CNS), in the pathogenesis of Alzheimer's disease (AD) remains elusive. Here, we developed a culture method for adult oligodendrocyte progenitor cells (aOPCs). Fibroblast growth factor 2 (FGF2) promotes survival and proliferation of NG2 aOPCs in a serum-free defined medium; a subpopulation (~5%) of plexin-B3 aOPCs was also found. Read More

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Planar refraction and lensing of highly confined polaritons in anisotropic media.

Nat Commun 2021 Jul 15;12(1):4325. Epub 2021 Jul 15.

Department of Physics, University of Oviedo, Oviedo, Spain.

Refraction between isotropic media is characterized by light bending towards the normal to the boundary when passing from a low- to a high-refractive-index medium. However, refraction between anisotropic media is a more exotic phenomenon which remains barely investigated, particularly at the nanoscale. Here, we visualize and comprehensively study the general case of refraction of electromagnetic waves between two strongly anisotropic (hyperbolic) media, and we do it with the use of nanoscale-confined polaritons in a natural medium: α-MoO. Read More

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Superluminal light propagation in a normal dispersive medium.

Opt Express 2021 Jun;29(13):20463-20476

The propagation of a Laguerre-Gaussian (LG) beam through a dispersive medium is investigated. The effect of the doughnut-like intensity profile of the probe LG beam on the group velocity is studied. We find an analytical expression for the group velocity out of the optical axis and compare with its projection onto the propagation axis. Read More

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Spatio-temporal controlled filamentation using higher order Bessel-Gaussian beams integrated in time.

Opt Express 2021 Jun;29(13):19362-19372

We demonstrate a new method for a systematic, dynamic, high-speed, spatio-temporal control of femtosecond light filamentation in BK7 as a particular example of nonlinear medium. This method is based on using coherent conjugate asymmetric Bessel-Gaussian beams to control the far-field intensity distribution and in turn control the filamentation location. Such spatio-temporal control allows every femtosecond pulse to have a unique intensity distribution that results in the generation of structured filamentation patterns on demand. Read More

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Agronomic performance and food safety of strawberry cultivated on a remediated sediment.

Sci Total Environ 2021 Jul 2;796:148803. Epub 2021 Jul 2.

Department of Agriculture, Food, Environment and Forestry, University of Florence, viale delle Idee 30, 50019 Sesto Fiorentino, Florence, Italy.

A marine sediment phytoremediated and homogenized by landfarming was tested for its potential recycle as growing media in horticulture. Two strawberry cultivars, Camarosa and Monterey, were grown on remediated sediment alone (TS100), commercial peat/pumice based growing medium (TS0) and a mixture 1:1 in volume of sediment and peat (TS50). Chemical fertility and strawberry production and safety of produced food were monitored for three consecutive productive seasons on the same growing media. Read More

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