17,183 results match your criteria measured separation

TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos.

J Anim Sci Biotechnol 2021 Aug 3;12(1):89. Epub 2021 Aug 3.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China.

Background: Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion. To derive greater economic benefit, farmers require more female offspring (does); however, the buck-to-doe offspring sex ratio is approximately 50%. At present, artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring. Read More

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Transport Diffusion of Linear Alkanes (C-C) through Thin Films of ZIF-8 as Assessed by Quartz Crystal Microgravimetry.

Langmuir 2021 Aug 2. Epub 2021 Aug 2.

Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.

We report uptake capacities and transport diffusivities, , for each of eight linear alkanes (ranging from C to C) in quartz crystal-supported films of solvent-evacuated ZIF-8. Analyses of the alkane uptake profiles revealed that the transport dynamics are governed by guest diffusion through metal-organic framework (MOF) (ZIF-8) crystallites rather than by rates of entry into films at the MOF/vapor interface. The obtained diffusivities range from just over 10 m/s to just under 10 m/s. Read More

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Clinical utility of Abbott Alinity hq extended red blood cell parameters in differentiating β-thalassemia trait and iron deficiency anemia.

Int J Lab Hematol 2021 Aug 1. Epub 2021 Aug 1.

Clinical Pathology Department, "San Giovanni Calibita" Fatebenefratelli Hospital, Rome, Italy.

Introduction: The objective of the study was to evaluate the performance of the Abbott Alinity hq advanced multi angle polarized scatter separation (MAPSS )-based optical RBC technology, for the differentiation between iron deficiency anemia (IDA) and β-thalassemia carrier status.

Methods: Four hundred and sixty-four samples were analyzed. 228 were healthy controls, 30 were β-thalassemia carriers, and 40 were IDA. Read More

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[NMR Study of the Discrimination of Enantiomers of Methamphetamine and Its Raw Materials Using Chiral Solvating Agents].

Yakugaku Zasshi 2021 ;141(8):1041-1048

Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences.

Some controlled substances, such as stimulants and narcotics, have asymmetric carbons in their molecules. Because the enantiomers do not always show the same pharmacological effects, and there are substances with different controls due to differences in their stereochemistry, a simple and unambiguous method for assessment of the composition of enantiomers is necessary. In this study, to develop a simple and rapid stereoscopic identification method for methamphetamine and its raw materials (ephedrine and pseudoephedrine), the H-NMR method was studied using three commercially available chiral solvating agents (CSAs); 1,1'-bi(2-naphthol)(BINOL), 2,2,2-trifluoro-1-(9-anthryl)ethanol (TFAE) and α-methoxy-α-(trifluoromethyl)phenylacetic acid (MTPA). Read More

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January 2021

Determinants of perceived health and unmet healthcare needs in universal healthcare systems with high gender equality.

BMC Public Health 2021 Jul 31;21(1):1488. Epub 2021 Jul 31.

Research Institute of McGill University Health Centre, Division of Clinical Epidemiology McGill University, Montreal, Canada.

Background: Patient attitudes about health and healthcare have emerged as important outcomes to assess in clinical studies. Gender is increasingly recognized as an intersectional social construct that may influence health. Our objective was to determine potential sex differences in self-reported overall health and access to healthcare and whether those differences are influenced by individual social factors in two relatively similar countries. Read More

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Looping-in complexation and ion partitioning in nonstoichiometric polyelectrolyte mixtures.

Sci Adv 2021 Jul 30;7(31). Epub 2021 Jul 30.

Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.

A wide variety of intracellular membraneless compartments are formed via liquid-liquid phase separation of charged proteins and nucleic acids. Understanding the stability of these compartments, while accounting for the compositional heterogeneity intrinsic to cellular environments, poses a daunting challenge. We combined experimental and theoretical efforts to study the effects of nonstoichiometric mixing on coacervation behavior and accurately measured the concentrations of polyelectrolytes and small ions in the coacervate and supernatant phases. Read More

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Amorphous NiS and FeOOH as bifunctional co-catalysts for oxygen reduction and phenol (water) oxidation over BiVO under visible light.

J Hazard Mater 2021 Jul 21;421:126650. Epub 2021 Jul 21.

State Key Laboratory of Silicon Materials and Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China. Electronic address:

Monoclinic BiVO (BiV) has been widely used as a photoanode for water oxidation, but rarely as a photocatalyst for organic oxidation due to slow reaction of O. In this work, BiV has been modified with poorly crystallized sFe and sNi, where sFe is FeOOH, and sNi is a mixture of Ni(OH) and polysulfide. Under light, sFe/BiV and sNi/BiV in aqueous solution were more active than BiV, respectively, not only for phenol oxidation but also for O reduction. Read More

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Scanning Thermal Microscopy and Ballistic Phonon Transport in Lateral Spin Valves.

Phys Rev Lett 2021 Jul;127(3):035901

School of Physics and Astronomy, University of Leeds, LS2 9JT Leeds, United Kingdom.

Using scanning thermal microscopy, we have mapped the spatial distribution of temperatures in an operating nanoscale device formed from a magnetic injector, an Ag connecting wire, and a magnetic detector. An analytical model explained the thermal diffusion over the measured temperature range (2-300 K) and injector-detector separation (400-3000 nm). The characteristic diffusion lengths of the Peltier and Joule heat differ remarkably below 60 K, a fact that can be explained by the onset of ballistic phonon heat transfer in the substrate. Read More

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Deuterium double quantum-filtered NMR studies of peripheral and optic nerves.

MAGMA 2021 Jul 30. Epub 2021 Jul 30.

School of Chemistry, Tel Aviv University, Tel Aviv, Israel.

Objective: Characterization of the nerve components by deuterium double quantum-filtered magnetization transfer (DQF-MT) NMR.

Methods: Nerves were equilibrated in deuterated saline and H single-pulse and H DQF-MT NMR spectra were measured, enabling the separation of the different water compartments, according to their quadrupolar splittings.

Results: Rat sciatic and brachial nerves and porcine optic nerve immersed in deuterated saline yielded H DQF spectra composed of three pairs of quadrupolar-split signals assigned to the water in the collagenous compartments and the myelin bilayer and one narrow signal assigned to the axonal water. Read More

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Association of Long Working Hours with Marital Status Changes in South Korea: A Cohort Study.

J Occup Environ Med 2021 Jul 27. Epub 2021 Jul 27.

Department of Occupational and Environmental Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Objective: This study investigated the relationship between long working hours and the incidence of divorce or separation among Korean female and male workers.

Methods: A cohort study was conducted in 125,108 Korean workers, married at baseline, who attended health examinations between 2012 and 2017 and had been followed annually or biennially until December 31, 2018. The weekly working hours and marital status changes were measured using self-administered questionnaires. Read More

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Assessing stress variations in children during the strange situation procedure: comparison of three widely used respiratory sinus arrhythmia estimation methods.

Physiol Meas 2021 Jul 29. Epub 2021 Jul 29.

Politecnico di Milano Dipartimento di Elettronica Informazione e Bioingegneria, Milano, ITALY.

Objective: The respiratory sinus arrhythmia (RSA) is a well-known marker of vagal activity that can be exploited to measure stress changes. RSA is usually estimated from heart rate variability (HRV). This study aims to compare the RSA obtained with three widely adopted methods showing their strengths and potential pitfalls. Read More

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Facile open-well immunofluorescence enhancement with coplanar-electrodes-enabled optoelectrokinetics and magnetic particles.

Biosens Bioelectron 2021 Jul 21;193:113527. Epub 2021 Jul 21.

Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan; Core Facility Center, National Cheng Kung University, Tainan, 701, Taiwan; Medical Device Innovation Center, National Cheng Kung University, Tainan, 701, Taiwan. Electronic address:

Electrokinetic manipulation has been proven powerful in enhancing the sensing capability of general-purpose biochips. However, the close-form configuration of biochips and the required use of low electric conductivity limit their practicability. In this study, an open-well microfluidic system facilitated with coplanar-electrodes-enabled optoelectrokinetic concentration and magnetic particles were therefore developed to overcome these challenges. Read More

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CuCoOx/BiVO multifunctional catalyst for organics degradation, water oxidation, and O reduction under visible light.

J Hazard Mater 2021 Jun 29;419:126515. Epub 2021 Jun 29.

State Key Laboratory of Silicon Materials and Department of Chemistry, Zhejiang University, Hangzhou 310027, China. Electronic address:

Monoclinic BiVO (BiV) is an excellent photoanode for water oxidation, but it is a poor photocatalyst for organic oxidation due to slow O reduction. Herein we report a 94-fold increased photocatalytic activity of BiV through a surface deposited CuCoOx. The model reaction was phenol degradation in aqueous solution under visible light. Read More

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Charge-transfer electronic states in organic solar cells: a TDDFT study.

Phys Chem Chem Phys 2021 Jul 29. Epub 2021 Jul 29.

Facultad de Química, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, CDMX 04510, Mexico.

The prediction of new organic photovoltaic materials in organic solar cells (OSCs) must include a precise description of charge-transfer states because they are involved in electron-transfer processes such as charge separation and charge recombination which govern the device efficiency. Also, as the experimental performance of an optoelectronic device is measured for nonequilibrium nanostructures, computational approaches need models that can incorporate morphology effects. Usually, this aspect is treated by molecular dynamics simulation (MDS) methodologies; however, methodologies and formalisms to calculate the electron-transfer processes are still controversial and sometimes do not connect their information with the phase morphologies. Read More

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Prevalence and course of anxiety disorders and symptoms from preschool to adolescence: a 6-wave community study.

J Child Psychol Psychiatry 2021 Jul 27. Epub 2021 Jul 27.

Department of Psychology, Norwegian University of Science and Technology, Trondheim, Norway.

Background: The rate of various anxiety disorders in early childhood and whether they continue into middle childhood or adolescence is not known. We therefore report on the prevalence and stability of DSM-5-defined anxiety disorders and their symptoms, capturing the period from preschool to adolescence.

Methods: By means of interviewer-based clinical interviews, anxiety was measured in a sample of Norwegian children at six measurement points from age 4 to 14 (n = 1,041). Read More

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An opportunity for primary prevention research in psychotic disorders.

Schizophr Res 2021 Jul 24. Epub 2021 Jul 24.

Rosalind Franklin University of Medicine and Science, United States of America. Electronic address:

An opportunity has opened for research into primary prevention of psychotic disorders, based on progress in endophenotypes, genetics, and genomics. Primary prevention requires reliable prediction of susceptibility before any symptoms are present. We studied a battery of measures where published data supports abnormalities of these measurements prior to appearance of initial psychosis symptoms. Read More

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Plutonium isotopes and radionuclides in corals around Weizhou land in Beibu Gulf, China.

Appl Radiat Isot 2021 Jul 22;176:109873. Epub 2021 Jul 22.

Guangxi Key Laboratory for Relativistic Astrophysics, School of Physical Science and Technology, Guangxi University, Nanning, 530004, China.

Plutonium isotopes in the coral were determined with chemical separation method using AG 1-X8 and AG-MP-1M anion exchange resins and sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) in order to elucidate the activity concentration and source of Pu around Weizhou land in Beibu Gulf, China. Furthermore, the activity concentrations of other radionuclides (U, Ra, Th, Cs, K and Pb) were measured by a HPGe spectrometer. The activity concentration of Pu in the coral is determined to be in the range of 8. Read More

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Effect of sugar metabolite methylglyoxal on equine lamellar explants: An ex vivo model of laminitis.

PLoS One 2021 27;16(7):e0253840. Epub 2021 Jul 27.

Department of Veterinary Science, University of Turin, Grugliasco (TO), Italy.

Laminitis is one of the most devastating diseases in equine medicine, and although several etiopathogenetic mechanisms have been proposed, few clear answers have been identified to date. Several lines of evidence point towards its underlying pathology as being metabolism-related. In the carbonyl stress pathway, sugars are converted to methylglyoxal (MG)-a highly reactive α-oxoaldehyde, mainly derived during glycolysis in eukaryotic cells from the triose phosphates: D-glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Read More

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Current Challenges and Recent Developments in Mass Spectrometry-Based Metabolomics.

Annu Rev Anal Chem (Palo Alto Calif) 2021 07 27;14(1):467-487. Epub 2021 Jul 27.

Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802, USA; email:

High-resolution mass spectrometry (MS) has advanced the study of metabolism in living systems by allowing many metabolites to be measured in a single experiment. Although improvements in mass detector sensitivity have facilitated the detection of greater numbers of analytes, compound identification strategies, feature reduction software, and data sharing have not kept up with the influx of MS data. Here, we discuss the ongoing challenges with MS-based metabolomics, including de novo metabolite identification from mass spectra, differentiation of metabolites from environmental contamination, chromatographic separation of isomers, and incomplete MS databases. Read More

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DNAzyme Sensor Uses Chemiluminescence Resonance Energy Transfer for Rapid, Portable, and Ratiometric Detection of Metal Ions.

Anal Chem 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

DNAzymes have emerged as an important class of sensors for a wide variety of metal ions, with florescence DNAzyme sensors as the most widely used in different sensing and imaging applications because of their fast response time, high signal intensity, and high sensitivity. However, the requirements of an external excitation light source and its associated power increase the cost and size of the fluorometer, making it difficult to be used for portable detections. To overcome these limitations, we report herein a DNAzyme sensor that relies on chemiluminescence resonance energy transfer (CRET) without the need for external light. Read More

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In silico process characterization for biopharmaceutical development following the Quality by Design concept.

Biotechnol Prog 2021 Jul 26:e3196. Epub 2021 Jul 26.

Karlsruhe Institute of Technology (KIT), Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe, Germany.

With the Quality by Design (QbD) initiative, regulatory authorities demand a consistent drug quality originating from a well-understood manufacturing process. This study demonstrates the application of a previously published mechanistic chromatography model to the in silico process characterization (PCS) of a monoclonal antibody polishing step. The proposed modeling workflow covered the main tasks of traditional PCS studies following the QbD principles, including criticality assessment of 11 process parameters and establishment of their proven acceptable ranges (PARs) of operation. Read More

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Antibacterial Properties of a Honeycomb-like Pattern with Cellulose Acetate and Silver Nanoparticles.

Materials (Basel) 2021 Jul 20;14(14). Epub 2021 Jul 20.

Department of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech Republic.

This study involved the preparation and characterization of structures with a honeycomb-like pattern (HCP) formed using the phase separation method using a solution mixture of chloroform and methanol together with cellulose acetate. Fluorinated ethylene propylene modified by plasma treatment was used as a suitable substrate for the formation of the HCP structures. Further, we modified the HCP structures using silver sputtering (discontinuous Ag nanoparticles) or by adding Ag nanoparticles in PEG into the cellulose acetate solution. Read More

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Influence of the Undercut Anchor Head Angle on the Propagation of the Failure Zone of the Rock Medium-Part II.

Materials (Basel) 2021 Jul 12;14(14). Epub 2021 Jul 12.

Department of Machine Design and Mechatronics, Faculty of Mechanical Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin, Poland.

Problems concerning the influence of the geometric parameters of an undercutting anchor on the range of the failure zone of rock medium during the pulling out of the anchor constitute one of the aspects that arouse the interest of authors due to attempts to use undercutting anchors in the process of rock lump separation. This method is considered an alternative to the existing methods of separation, especially in special cases of mining technologies. This article presents the results of numerical investigations into the effect of changes in the head geometry that occur as a result of wear on the conical part of the undercutting anchor and the extent of failure of the rock medium during its pulling out. Read More

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The aerodynamics of flying snake airfoils in tandem configuration.

J Exp Biol 2021 Jul 23;224(14). Epub 2021 Jul 23.

Department of Biomedical Engineering and Mechanics, Virginia Tech, Blacksburg, VA 24061, USA.

Flying snakes flatten their body to form a roughly triangular cross-sectional shape, enabling lift production and horizontal acceleration. While gliding, they also assume an S-shaped posture, which could promote aerodynamic interactions between the fore and the aft body. Such interactions have been studied experimentally; however, very coarse models of the snake's cross-sectional shape were used, and the effects were measured only for the downstream model. Read More

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A derivatization and microextraction procedure with organic phase solidification on a paper template: Spectrofluorometric determination of formaldehyde in milk.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 8;263:120160. Epub 2021 Jul 8.

Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, St. Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya nab., St. Petersburg 199034, Russia.

A derivatization and air-assisted dispersive liquid-liquid microextraction procedure with organic phase solidification on a paper template was developed for the first time. The procedure was used for the spectrofluorometric determination of formaldehyde in milk samples. The Hantzsch reaction of formaldehyde with acetylacetone in the presence of ammonia to form a derivative (3,5-diacetyl-1,4-dihydrolutidine) was implemented for the microextraction and detection of analyte. Read More

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Geometric Optimization of Bismuth Vanadate Core-Shell Nanowire Photoanodes using Atomic Layer Deposition.

ACS Appl Mater Interfaces 2021 Jul 20. Epub 2021 Jul 20.

Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

In this study, systematic geometric tuning of core-shell nanowire (NW) architectures is used to decouple the contributions from light absorption, charge separation, and charge transfer kinetics in photoelectrochemical water oxidation. Core-shell-shell NW arrays were fabricated using a combination of hydrothermal synthesis of ZnO and atomic layer deposition (ALD) of SnO and BiVO. The length and spacing of the NW scaffold, as well as the BiVO film thickness, were systematically tuned to optimize the photoelectrochemical performance. Read More

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Simultaneous quantitation of inorganic ions in oligonucleotides using mixed-mode liquid chromatography coupled with a charged aerosol detector.

J Pharm Biomed Anal 2021 Jul 8;204:114244. Epub 2021 Jul 8.

Department of Analytical Development, Biogen, 225 Binney St, Cambridge, MA 02142, USA.

The amounts of inorganic ions (e.g. sodium, potassium, magnesium, calcium, chloride, and phosphate) in intrathecally administered oligonucleotide drug products need to be controlled in order to meet the final target formulation composition. Read More

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Biocompatibility Parameters with Standard and Increased Dose of Citrate in Hemodialysis-A Randomized Trial.

J Clin Med 2021 Jul 4;10(13). Epub 2021 Jul 4.

Department of Nephrology, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia.

Background: The dose of citrate needed in regional citrate anticoagulation (RCA) to achieve optimal biocompatibility is unknown. We performed a randomized trial comparing two doses (ACTRN12613001340729).

Methods: In 30 patients a single hemodialysis with either standard (2. Read More

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Effect of patient thickness on acute gastrointestinal toxicities following radiotherapy for prostate cancer.

Rep Pract Oncol Radiother 2021 9;26(3):352-359. Epub 2021 Jun 9.

Department of Radiography, Faculty of Health Sciences, University of Malta, Msida, Malta.

Background: There is conflicting literature regarding the effect of patient size on radiotherapy toxicities. This study aimed to determine whether there is any association between patient thickness and severity and incidence of acute GI toxicities of prostate cancer patients receiving VMAT radiotherapy. The impact of confounding factors was also examined: rectal dose, age and lymph node irradiation. Read More

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Smoothing temperature and ratio of casein to whey protein: Two tools to improve nonfat stirred yogurt properties.

J Dairy Sci 2021 Jul 15. Epub 2021 Jul 15.

Department of Food Sciences, Université Laval, Quebec City, QC, Canada, G1V 0A6; STELA Dairy Research Centre and Institute of Nutrition and Functional Foods, Université Laval, Quebec City, QC, Canada, G1V 0A6. Electronic address:

Consumers are not always ready to compromise on the loss of texture and increased syneresis that nonfat stirred yogurts display compared with yogurts that contain fat. In this study, we investigated milk protein composition and smoothing temperature as a means to control nonfat yogurt microstructure, textural properties, and syneresis. Yogurts were prepared with different ratios of casein to whey protein (R1. Read More

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