22,785 results match your criteria measured dose-dependent

Pro-oxidant and degenerative effects of haloperidol under inflammatory conditions in rat; the involvement of SIRT1 and NF-κB signaling pathways.

Vet Res Forum 2021 15;12(2):175-183. Epub 2021 Jun 15.

Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

The present study was conducted to evaluate the effects of different doses of haloperidol (HP) on induction of oxidative stress in blood and liver cell degeneration in comparison with influences of HP pre-treatment on inflammatory process induced by intraperitoneal (IP) administration of lipopolysaccharide (LPS). One hundred twenty male albino Wistar rats were randomly divided into eight groups (15 in each), including: Control group, LPS group, three groups as HP administration in three divided doses (0.50, 1. Read More

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Liraglutide improves pancreatic islet β cell apoptosis in rats with type 2 diabetes mellitus by inhibiting the IKKε/NF-κB pathway.

Eur Rev Med Pharmacol Sci 2021 Jul;25(14):4818-4828

Department of Endocrinology, Affiliated Ren He Hospital of China Three Gorges University, The Second Clinical Medical College of China Three Gorges University, Yichang, China.

Objective: The purpose of this study was to explore the effect of liraglutide on pancreatic islet β cell apoptosis in rats with type 2 diabetes mellitus (T2DM) and the potential mechanisms.

Materials And Methods: SD rats were randomly divided into control group, model group, and liraglutide groups (200 and 100 μg/(kg·d)). Rats were fed with high sugar and high-fat diet for 8 weeks, and then streptozotocin (STZ) 40 mg/kg was intraperitoneally injected to establish T2DM model. Read More

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Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro.

Expo Health 2021 Jul 25:1-11. Epub 2021 Jul 25.

Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME 04005 USA.

Microplastics are ubiquitous environmental pollutants that are a growing concern to many ecosystems, as well as human health. Many of the effects of microplastics on mammalian cells and tissues remain unknown. To address this, we treated L929 murine fibroblasts and Madin-Darby canine kidney (MDCK) epithelial cell lines with 1 μg/mL, 10 μg/mL, or 20 μg/mL of polyethylene (PE) or polystyrene (PS) microspheres in vitro for 6 and 24 h and measured the resulting changes in cell viability, metabolism, and transcriptional expression of inflammatory cytokines and antioxidant enzymes. Read More

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extract protects human neuroblastoma SH-SY5Y cells against oxidative glutamate toxicity by activating redoxosome-p66Shc.

Exp Ther Med 2021 Sep 5;22(3):951. Epub 2021 Jul 5.

Sydney Pharmacy School, The University of Sydney, Sydney, New South Wales 2006, Australia.

extract (GBE), a traditional Chinese herbal medicine component, is widely used to alleviate symptoms of neurodegenerative diseases. It has been confirmed that GBE exerts its pharmacological effect mainly due to its antioxidant activity; however, the molecular mechanism responsible for this effect remains unclear. The aim of the present study was to investigate the detailed mechanism of GBE, the main component of dropping medicine, against oxidative glutamate toxicity in human neuroblastoma SH-SY5Y cells. Read More

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September 2021

Topical treatment with mastic (resin from Pistacia lentiscus) elicits anti-inflammatory and anti-pruritic responses by modulating keratinocyte activation in a mouse model of allergic dermatitis.

Phytomedicine 2021 Jul 19;91:153679. Epub 2021 Jul 19.

Laboratory of Veterinary Pharmacology, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa, Japan. Electronic address:

Background: As the number of patients with skin allergies, including atopic dermatitis, has increased rapidly, therapeutic options such as anti-IL-31 antibody and Janus kinase inhibitor have been developed recently. However, many concerns remain regarding the adverse effects and cost of these drugs; therefore, development of supplements that could support the effect of therapeutic agents is always required.

Purpose: The aim of this study was to develop preventive and supportive options for skin allergies by focusing on a natural product called "Mastic". Read More

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Functional and Anatomical Characterization of Corticotropin-Releasing Factor Receptor Subtypes of the Rat Spinal Cord Involved in Somatic Pain Relief.

Mol Neurobiol 2021 Jul 31. Epub 2021 Jul 31.

Department of Anesthesiology and Operative Intensive Care Medicine, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charité - Universitätsmedizin Berlin, Charité Campus Benjamin Franklin, Hindenburgdamm 30, 12203, Berlin, Germany.

Corticotropin-releasing factor (CRF) orchestrates our body's response to stressful stimuli. Pain is often stressful and counterbalanced by activation of CRF receptors along the nociceptive pathway, although the involvement of the CRF receptor subtypes 1 and/or 2 (CRF-R1 and CRF-R2, respectively) in CRF-induced analgesia remains controversial. Thus, the aim of the present study was to examine CRF-R1 and CRF-R2 expression within the spinal cord of rats with Freund's complete adjuvant-induced unilateral inflammation of the hind paw using reverse transcriptase polymerase chain reaction, Western blot, radioligand binding, and immunofluorescence confocal analysis. Read More

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DNA damage assessment in peripheral blood of Swiss albino mice after combined exposure to volatile anesthetics and 1 or 2 Gy radiotherapy .

Int J Radiat Biol 2021 Jul 30:1-29. Epub 2021 Jul 30.

Institute for Medical Research and Occupational Health, Zagreb, Croatia.

Purpose: Patient immobilization by general volatile anesthesia (VA) may be necessary during medical radiology treatment, and its use has increased in recent years. Although ionizing radiation (IR) is a well-known genotoxic and cytotoxic agent, and VA exposure has caused a range of side effects among patients and occupationally exposed personnel, there are no studies to date comparing DNA damage effects from combined VA and single fractional IR dose exposure.

Material And Methods: We investigate whether there is a difference in white blood cells DNA damage response (by the alkaline comet assay) in 185 healthy Swiss albino mice divided into 37 groups, anesthetized with isoflurane/sevoflurane/halothane and exposed to 1 or 2 Gy of IR. Read More

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Chalcogenide nanoparticles and organic photosensitizers for synergetic antimicrobial photodynamic therapy.

J Mater Chem B 2021 Jul 30. Epub 2021 Jul 30.

Department of Microbiology, Hospital Universitario Miguel Servet, 50009 Zaragoza, Spain and Department of Microbiology, Pediatrics, Radiology and Public Health, University of Zaragoza, 50009 Zaragoza, Spain and Aragon Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.

Synergistic antimicrobial effects were observed for copper sulfide (CuS) nanoparticles together with indocyanine green (ICG) in the elimination of wild type pathogenic bacteria (Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853) and also opportunistic fungal infective yeast (Candida albicans ATCC 10231). Furthermore, large antibacterial effects were observed for clinical isolates of Methicillin-resistant S. aureus (MRSA) PFGE strain-type USA300. Read More

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COVID-19 vaccinations are associated with reduced fatality rates: Evidence from cross-county quasi-experiments.

J Glob Health 2021 17;11:05019. Epub 2021 Jul 17.

Research Center for Epidemic Prevention, National Yang Ming Chiao Tung University, Taipei, Taiwan.

Background: Scientists have demonstrated the efficacy of vaccines against severe acute respiratory syndrome coronavirus 2 in randomized controlled trials. However, the extent to which reductions in COVID-19 case fatality ratio (CFR) are attributable to mass vaccination in the real world remains unclear. This study evaluated the association of COVID-19 vaccine coverage with CFR on a global scale. Read More

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Decarboxylated osteocalcin, a possible drug for type 2 diabetes, triggers glucose uptake in MG63 cells.

World J Diabetes 2021 Jul;12(7):1102-1115

Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, Liaoning Province, China.

Background: Uncarboxylated osteocalcin (GluOC) has been reported to improve glucose metabolism, prevent type 2 diabetes, and decrease the severity of obesity in mice with type 2 diabetes. GluOC can increase glucose uptake in a variety of cells. Glucose metabolism is the main source of energy for osteoblast proliferation and differentiation. Read More

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Hemodynamic impact of increasing time between fentanyl and propofol administration during anesthesia induction: a randomised, clinical trial.

Braz J Anesthesiol 2021 Jul 26. Epub 2021 Jul 26.

Hospital Central de la Defensa Gómez Ulla-IMIDEF, Critical Care and Pain Unit, Department of Anesthesia, Madrid, Spain.

Background And Objective: Anesthesia induction can produce severe propofol dose-dependent hypotension. Fentanyl coadministration reduces the catecholaminergic response to orotracheal intubation allowing propofol dose reduction. The aim of this study is to determine whether the hemodynamic response is improved by increasing the time between fentanyl and propofol administration and reducing the dose of the latter without increasing the time to achieve optimal hypnosis. Read More

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[The Effects of Ginsenoside Compound K on PDGF-BB-Induced PASMCs Proliferation and Phenotypic Conversion of Pulmonary Arterial Smooth Muscle Cells].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Jul;52(4):643-648

Department of Pediatrics, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

Objective: To explore the inhibitory effects of ginsenoside compound K (CK) on pulmonary arterial smooth muscle cells (PASMCs) proliferation and phenotypic conversion and investigate its related mechanisms.

Methods: PASMCs cultured were examined in the study. They were induced with platelet-derived growth factor-BB (PDGF-BB) and then treated with CK. Read More

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Accommodative response to ocular surface pain.

Clin Exp Optom 2021 Jul 28:1-7. Epub 2021 Jul 28.

School of Optometry and Vision Science, University of Waterloo, Waterloo, Canada.

Clinical Relevance: The discovery of an accommodative response to ocular surface stimulation could inform clinicians and patients that optical effects may occur due to ocular discomfort and perhaps an assessment of the accommodative system after carrying out interventions impacting the ocular surface, may be warranted.

Background: There have been no previous reports evaluating the effect of noxious stimulation on accommodation. Here, the accommodative response of healthy participants after the application of noxious corneal stimulation is characterised. Read More

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Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells Through Akt/MDM2/p53 Signaling Pathway.

Chin J Integr Med 2021 Jul 28. Epub 2021 Jul 28.

Department of Biological science and Biotechnology, College of Life science and Nano Technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.

Objective: To determine the effect of Zanthoxylum piperitum extracet (ZPE) on apoptosis and analyze anticancer substances in ZPE, changes in proteins related to apoptosis, and pathological changes in tumors in mouse.

Methods: Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZPE dose, with 5 in each group. AGS gastric carcinoma cells (1 × 10 cells/200 µL) were subcutaneously injected into the flank of each mouse. Read More

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Anticancer Effects of Cinnamaldehyde through Inhibition of ErbB2/HSF1/LDHA Pathway in 5637 Cell Line of Bladder Cancer.

Anticancer Agents Med Chem 2021 Jul 26. Epub 2021 Jul 26.

Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: The growing prevalence of bladder cancer worldwide has become a major concern for the researchers, and the side effects of chemotherapy drugs have always been a major problem in cancer treatment. Cinnamaldehyde, the active ingredient in the Cinnamon plant, has long been considered with anti-oxidant and anti-inflammatory effects.

Methods: Bladder cancer 5637 cell lines were treated with the different concentrations of Cinnamaldehyde. Read More

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Taurine Upregulates miRNA-122-5p Expression and Suppresses the Metabolizing Enzymes of Glycolytic Pathway in Hepatocellular Carcinoma.

Mol Biol Rep 2021 Jul 27. Epub 2021 Jul 27.

Department of Zoology, Faculty of Science, Cairo University, Giza, 12613, Egypt.

Background: Hepatocellular carcinoma (HCC) is a complicated disease with a poor prognosis and high mortality rates. The prevention, control, diagnosis, and treatment of liver cancer have become vital focuses in healthcare research.

Aim: This study aimed to evaluate the in vitro effect of taurine (Tau) on the expression of miR-122-5p that targets some limiting glycolytic enzymes and affects the overall glycolytic pathway in HepG2 cells. Read More

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[Rose-scented geranium essential oil from Algeria (Pelargonium graveolens L'Hérit.): Assessment of antioxidant, anti-inflammatory and anticancer properties against different metastatic cancer cell lines].

Ann Pharm Fr 2021 Jul 23. Epub 2021 Jul 23.

The Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, 12144 NY, USA.

Introduction: The study of natural products is one of the strategies implemented for the discovery of new compounds that can be used in cancer therapy. Aromatic herbs and medicinal plants found in Algeria and their anti-angiogenesis and cytotoxic potentials against cancer have not been much explored.

Objectives: Our work aimed to evaluate the antioxidant, anti-inflammatory and anticancer properties of the essential oil (EO) extracted from rose-scented geranium (Pelargonium graveolens) and its major (citronellol) and characteristic (linalool) constituents. Read More

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Reversine inhibits proliferation, invasion and migration and induces cell apoptosis in gastric cancer cells by downregulating TTK.

Exp Ther Med 2021 Sep 30;22(3):929. Epub 2021 Jun 30.

Department of Liver-Gallbladder and Gastric Diseases, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, P.R. China.

Reversine (Rev) has been used for the treatment of a number of cancers. However, there have been no previous reports for the use of Rev for gastric cancer (GC). The aim of the present study was to investigate the effect of Rev on cell proliferation, migration, invasion and cell apoptosis in human GC cells and TTK expression. Read More

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September 2021

Novel Indigenous Probiotic Lactobacillus reuteri Strain Produces Anti-biofilm Reuterin against Pathogenic Periodontal Bacteria.

Eur J Dent 2021 Jul 24. Epub 2021 Jul 24.

Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Indonesia.

Objective:  The aim of this study was to evaluate the effect of reuterin produced by a novel probiotic strain of against periodontal biofilms.

Materials And Methods:   LC382415 (an indigenous Indonesian strain) was cultured in Man, Rogosa, and Sharpe (MRS) agar in anaerobic conditions for 24 hours. To isolate reuterin, was suspended in 300-mM glycerol in MRS broth and incubated under anaerobic conditions for 3 hours, and the supernatant fraction was filtered. Read More

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Effects of Chronic Low-Dose Internal Radiation on Immune-Stimulatory Responses in Mice.

Int J Mol Sci 2021 Jul 7;22(14). Epub 2021 Jul 7.

Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

The Linear-No-Threshold (LNT) model predicts a dose-dependent linear increase in cancer risk. This has been supported by biological and epidemiological studies at high-dose exposures. However, at low-doses (LDR ≤ 0. Read More

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Evaluation of Maghemite Nanoparticles-Induced Developmental Toxicity and Oxidative Stress in Zebrafish Embryos/Larvae.

Biol Trace Elem Res 2021 Jul 23. Epub 2021 Jul 23.

Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, 641046, India.

Maghemite nanoparticles ([Formula: see text] NPs) have a wide array of applications in various industries including biomedical field. There is an absence of legislation globally for the regulation of the production, use, and disposal of such NPs as they are eventually dumped into the environment where these NPs might affect the living systems. This study evaluates the effect of the [Formula: see text] NP-induced developmental toxicity in zebrafish embryos/larvae. Read More

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Fibromyalgianess and glucocorticoid persistence among patients with rheumatoid arthritis.

Rheumatology (Oxford) 2021 Jul 22. Epub 2021 Jul 22.

Internal Medicine/Rheumatology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Objectives: Over 1/3 of patients with rheumatoid arthritis (RA) exhibit evidence of fibromyalgianess, which is associated with higher rates of disability and inadequate responsiveness to RA treatment. Patients with RA often remain on glucocorticoids long-term, despite the known risk of dose-dependent morbidity. We undertook this study to examine the relationship between fibromyalgianess and glucocorticoid persistence among RA patients. Read More

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Population Pharmacokinetics of Favipiravir in Patients with COVID-19.

CPT Pharmacometrics Syst Pharmacol 2021 Jul 22. Epub 2021 Jul 22.

Department of Pharmacy, Kobe City Hospital Organization, Kobe City Medical Center General Hospital, Kobe, 650-0047, Japan.

The antiretroviral drug favipiravir inhibits RNA-dependent RNA polymerase. It has been developed for the treatment of novel coronavirus (SARS-CoV-2) infection disease (COVID-19). However, its pharmacokinetics in patients with COVID-19 is poorly understood. Read More

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Proteinase 3 contributes to endothelial dysfunction in an experimental model of sepsis.

Exp Biol Med (Maywood) 2021 Jul 22:15353702211029284. Epub 2021 Jul 22.

Centre for Critical Illness Research, 151158Lawson Health Research Institute, Lawson Health Research Institute, London, N6A 5W9, Canada.

In sepsis-induced inflammation, polymorphonuclear neutrophils (PMNs) contribute to vascular dysfunction. The serine proteases proteinase 3 (PR3) and human leukocyte elastase (HLE) are abundant in PMNs and are released upon degranulation. While HLE's role in inflammation-induced endothelial dysfunction is well studied, PR3's role is largely uninvestigated. Read More

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Anti-edema effect of leaf extract following traumatic brain injury: Role of pro-inflammatory cytokines.

Avicenna J Phytomed 2021 Jul-Aug;11(4):380-393

Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Objective: Based on anti-inflammatory effects of , the effect of aqueous extract of this plant on brain edema and changes in some pro-inflammatory cytokines was investigated after traumatic brain injury (TBI).

Materials And Methods: In this study, adult male Wistar rats were divided into 5 groups: Sham, TBI, vehicle (Veh), and low dose (LA) and high dose (HA) . The vehicle and aqueous extract of were injected intraperitoneally 30 min after induction of diffuse TBI by Marmarou's method. Read More

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September 2020

Cells Stimulated with More Than One Toll-Like Receptor-Ligand in the Presence of a MyD88 Inhibitor Augmented Interferon- via MyD88-Independent Signaling Pathway.

Viral Immunol 2021 Jul 20. Epub 2021 Jul 20.

Department of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.

Host exposure to pathogens engage multiple pathogen recognition receptors (PRRs) including toll-like receptors (TLRs); recruit intracellular signaling adaptor proteins primarily myeloid differentiation primary response protein 88 (MyD88) for activating downstream signaling cascades, which culminate in the production of type I interferons (IFNs), proinflammatory cytokines, and chemokines; and impede pathogen replication and dissemination. However, recent studies highlight that absence of MyD88 increased antiviral type I IFN induction, and MyD88 mice showed a higher survival rate compared with the low survival rate of the MyD88 mice, implicating MyD88 limits antiviral type I IFN response. As a single infectious agent may harbor multiple PRR agonists, which trigger different sets of TLR-initiated immune signaling, we examined whether MyD88 inhibition during stimulation of cells with more than one TLR-ligand would augment type I IFN. Read More

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Transcutaneous penetration of a single-chain variable fragment (scFv) compared to a full-size antibody: potential tool for atopic dermatitis (AD) treatment.

Allergy Asthma Clin Immunol 2021 Jul 19;17(1):73. Epub 2021 Jul 19.

Unité Mixte de Recherche CNRS, 7276-INSERM U1262-Université de Limoges, CBRS, 2 rue du Dr Marcland, 87025, Limoges, France.

Currently, several biologics are used for the treatment of cutaneous pathologies such as atopic dermatitis (AD), psoriasis or skin cancers. The main administration routes are subcutaneous and intravenous injections. However, little is known about antibody penetration through the skin. Read More

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Release of secretory immunoglobulin A by submandibular gland via β adrenergic receptor stimulation.

Arch Oral Biol 2021 Jul 14;129:105209. Epub 2021 Jul 14.

Laboratory of Molecular Biology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan. Electronic address:

Objective: Secretory immunoglobulin A (sIgA) is important for mucosal immunity due to the inhibition of pathogen infection. The submandibular gland is known to secrete more sIgA than the parotid and sublingual glands. In this study, we focused on the relationship between the secretion of accumulated intracellular sIgA and β-adrenergic receptor stimulation, and clarified the autonomic regulatory mechanism of sIgA secretion in submandibular gland cells using dispersed gland cells. Read More

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Aircraft noise exposure drives the activation of white blood cells and induces microvascular dysfunction in mice.

Redox Biol 2021 Jul 8;46:102063. Epub 2021 Jul 8.

Department of Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany; German Center for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Mainz, Germany. Electronic address:

Epidemiological studies showed that traffic noise has a dose-dependent association with increased cardiovascular morbidity and mortality. Whether microvascular dysfunction contributes significantly to the cardiovascular health effects by noise exposure remains to be established. The connection of inflammation and immune cell interaction with microvascular damage and functional impairment is also not well characterized. Read More

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Protective effects of Quercetin and Resveratrol on aging markers in kidney under high glucose condition: in vivo and in vitro analysis.

Mol Biol Rep 2021 Jul 17. Epub 2021 Jul 17.

Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Background: Resveratrol and Quercetin as powerful herbal antioxidant reduce kidney complications of diabetes. The purpose of this study was to evaluate the protective effects of Quercetin and Resveratrol on aging markers in high glucose condition.

Methods And Results: In this study, HEK-293 cells and kidney tissue of male Wistar rats were used in hyperglycemia condition. Read More

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