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OPTIMIZING A PLATELET-RICH PLASMA CONCENTRATION PROTOCOL FOR SOUTH AMERICAN SEA LIONS ().

J Zoo Wildl Med 2021 Sep;52(3):956-965

Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera (Científic)1B, 46013 Valencia, Spain.

Accelerated healing in wild or captive South American sea lions () is a key tool to help minimize infection and complications associated with open wounds, dental disease, and ocular pathology. Platelet-rich plasma (PRP) is an autogenous source for growth factors based on platelet concentration, which can be obtained by centrifuging whole blood collected in sodium citrate anticoagulant. Currently, there are well-defined PRP concentration protocols for humans and most domestic companion animal species. Read More

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September 2021

Simple oligonucleotide-based multiplexing of single-cell chromatin accessibility.

Mol Cell 2021 Oct;81(20):4319-4332.e10

Department of Genetics, UT MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate School of Biological Sciences, UT MD Anderson Cancer Center, Houston, TX 77030, USA; Department of Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston, TX 77030, USA. Electronic address:

Microdroplet single-cell ATAC-seq is widely used to measure chromatin accessibility, however, highly scalable and simple sample multiplexing procedures are not available. Here, we present a transposome-assisted single nucleus barcoding approach for ATAC-seq (SNuBar-ATAC) that utilizes a single oligonucleotide adaptor for multiplexing samples during the existing tagmentation step and does not require a pre-labeling procedure. The accuracy and scalability of SNuBar-ATAC was evaluated using cell line mixture experiments. Read More

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October 2021

Development of an Automated, Non-Enzymatic Nucleic Acid Amplification Test.

Micromachines (Basel) 2021 Sep 30;12(10). Epub 2021 Sep 30.

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USA.

Among nucleic acid diagnostic strategies, non-enzymatic tests are the most promising for application at the point of care in low-resource settings. They remain relatively under-utilized, however, due to inadequate sensitivity. Inspired by a recent demonstration of a highly-sensitive dumbbell DNA amplification strategy, we developed an automated, self-contained assay for detection of target DNA. Read More

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September 2021

Prevalence of Locomotive Organ Impairment and Associated Factors among Middle-Aged and Older People in Nan Province, Thailand.

Int J Environ Res Public Health 2021 Oct 15;18(20). Epub 2021 Oct 15.

Graduate School of Public Health, Teikyo University, Tokyo 173-8605, Japan.

While locomotive organ impairment among older people is attracting worldwide attention, this issue has not yet been widely investigated in Thailand. This study aimed to measure locomotive organ impairment prevalence and identify the determinants of locomotive function decline among middle-aged and older people in Nan Province, Thailand. This cross-sectional study included anthropometric measurements, a two-step test to investigate locomotive function, and a structured questionnaire to obtain socio-demographic and related information. Read More

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October 2021

Dynein-Inspired Multilane Exclusion Process with Open Boundary Conditions.

Entropy (Basel) 2021 Oct 14;23(10). Epub 2021 Oct 14.

Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Motivated by the sidewise motions of dynein motors shown in experiments, we use a variant of the exclusion process to model the multistep dynamics of dyneins on a cylinder with open ends. Due to the varied step sizes of the particles in a quasi-two-dimensional topology, we observe the emergence of a novel phase diagram depending on the various load conditions. Under high-load conditions, our numerical findings yield results similar to the TASEP model with the presence of all three standard TASEP phases, namely the low-density (LD), high-density (HD), and maximal-current (MC) phases. Read More

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October 2021

Results of 15 Years of Precision Feeding of Hyper Prolific Gestating Sows.

Authors:
Nathalie Quiniou

Animals (Basel) 2021 Oct 8;11(10). Epub 2021 Oct 8.

IFIP-Institut du Porc, BP35104, CEDEX, 35651 Le Rheu, France.

The increase in prolificacy at weaning is less than that at farrowing due to increased loss of piglets. As a result, farmers focus more on solutions that can prevent difficult parturition or a decrease in milk production. The body condition of the sow influences both factors. Read More

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October 2021

Time-Driven Activity-Based Costing of CT-Guided vs MR-Guided Prostate SBRT.

Appl Radiat Oncol 2021 Sep 5;10(3):33-40. Epub 2021 Oct 5.

Department of Radiation Oncology, University of California Los Angeles.

Background And Purpose: Stereotactic body radiation therapy (SBRT) has become a standard-of-care option for localized prostate cancer. While prostate SBRT has traditionally been delivered using computed-tomography-guided radiation therapy (CTgRT), MR-imaging-guided radiation therapy (MRgRT) is now available. MRgRT offers real-time soft-tissue visualization and ease of adaptive planning, obviating the need for fiducial markers, and potentially allowing for smaller planning target volume (PTV) margins. Read More

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September 2021

Measuring protein biomarker concentrations using antibody tagged magnetic nanoparticles.

Biomed Phys Eng Express 2020 Nov 3;6(6). Epub 2020 Nov 3.

Thayer School of Engineering, Dartmouth College, Hanover, United States of America.

Under physiological conditions biomarker concentrations tend to rise and fall over time e.g. for inflammation. Read More

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November 2020

Reconciling validity and challenges of patient comfort and understanding: Guidelines to patient-oriented questionnaires.

Health Expect 2021 Oct 20. Epub 2021 Oct 20.

Faculté des sciences infirmières, Université de Montréal, Montreal, Canada.

Background: Patient-reported outcome measures (PROMs) are widely recognized as important tools for achieving a patient-centred approach in health research. While PROMs are subject to several stages of validation during development, even questionnaires with robust psychometric properties may challenge patient comfort and understanding.

Aim: Building on the experience of patient engagement in the PriCARE research programme, this paper outlines the team's response to concerns raised by patient partners regarding the administration of the questionnaire. Read More

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October 2021

Ensemble classification and segmentation for intracranial metastatic tumors on MRI images based on 2D U-nets.

Sci Rep 2021 Oct 19;11(1):20634. Epub 2021 Oct 19.

Institute of Statistics, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.

The extraction of brain tumor tissues in 3D Brain Magnetic Resonance Imaging (MRI) plays an important role in diagnosis before the gamma knife radiosurgery (GKRS). In this article, the post-contrast T1 whole-brain MRI images had been collected by Taipei Veterans General Hospital (TVGH) and stored in DICOM format (dated from 1999 to 2018). The proposed method starts with the active contour model to get the region of interest (ROI) automatically and enhance the image contrast. Read More

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October 2021

Heat insulation effect in solar radiation of polyurethane powder coating nanocomposite.

Sci Rep 2021 Oct 19;11(1):20665. Epub 2021 Oct 19.

Department of Resin and Additives, Institute for Color Science and Technology, Tehran, 1668836471, Iran.

This study aims to improve polyurethane-based coating by modified zirconium oxide and aluminum oxide nanoparticles for preparing thin polymeric heat insulation coatings. In the first step, the nanoparticles were chemically modified with the silane coupling agent. Then, three different weight percent of modified nanoparticles (1, 3, and 5% w/w) were mixed with polyurethane, to prepare the nanocomposites, which were coated on metallic plate samples. Read More

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October 2021

Development of a tool to measure holistic reflection in midwifery students and midwives.

Women Birth 2021 Oct 16. Epub 2021 Oct 16.

School of Nursing and Midwifery, Griffith University, University Drive, Meadowbrook, Queensland, 4131, Australia.

Background: Holistic reflection encompasses reflection as a continuum, incorporating critical reflection, reflexivity, critical thinking and a whole-of-person approach. The development of holistic reflection in midwifery students and midwives is rarely measured in a standardised way.

Aim: To develop and test a tool to measure holistic reflection in midwifery students and midwives, for use in pre-registration and post-graduate education, and research. Read More

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October 2021

An exploration of potential output measures to assess efficiency and productivity for labour and birth in Australia.

BMC Pregnancy Childbirth 2021 Oct 19;21(1):703. Epub 2021 Oct 19.

College of Public Health, Medical and Veterinary Science, James Cook University, 1 James Cook Drive, Townsville, Queensland, 4811, Australia.

Background: In maternity services, as in other areas of healthcare, increasing emphasis is placed on improving "efficiency" or "productivity". The first step in any efficiency and productivity analysis is the selection of relevant input and output measures. Within healthcare quantifying what is produced (outputs) can be difficult. Read More

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October 2021

Graduate training during the COVID-19 pandemic: North American genetic counseling students' challenges, intolerance of uncertainty, and psychological well-being.

J Genet Couns 2021 Oct 19. Epub 2021 Oct 19.

Department of Human Genetics, University of Michigan, Ann Arbor, Michigan, USA.

The COVID-19 pandemic has upended genetic counseling (GC) graduate students' lives, as they have been forced to transition, manage, and acclimate to life during a pandemic. The current study is a first step in understanding their lived experiences during this historic, global event. We investigated academic and personal challenges, intolerance of uncertainty, and psychological well-being of GC students (n = 248) who trained during the pandemic (GC-COV cohort) and those who did not (GC-NoCOV cohort). Read More

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October 2021

RgCop-A regularized copula based method for gene selection in single cell rna-seq data.

PLoS Comput Biol 2021 Oct 19;17(10):e1009464. Epub 2021 Oct 19.

Machine Intelligence Unit, Indian Statistical Institute, Kolkata, India.

Gene selection in unannotated large single cell RNA sequencing (scRNA-seq) data is important and crucial step in the preliminary step of downstream analysis. The existing approaches are primarily based on high variation (highly variable genes) or significant high expression (highly expressed genes) failed to provide stable and predictive feature set due to technical noise present in the data. Here, we propose RgCop, a novel regularized copula based method for gene selection from large single cell RNA-seq data. Read More

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October 2021

Did You Say My Name? Congruency of 12-month-old Infants' Behavioral and Cardiac Responses to Name.

Biol Psychol 2021 Oct 15:108207. Epub 2021 Oct 15.

Department of Psychological Sciences, Purdue University 703 Third Street, West Lafayette, IN 47907, USA. Electronic address:

Reduced orienting to name is an early behavioral risk marker for neurodevelopmental disorders. However, individual instances of infants' behavioral responses to name are limited in both reliability and predictive validity. Physiological responses such as heart rate (HR) deceleration may serve as more sensitive metrics than behavioral methods. Read More

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October 2021

Preoperative Psychosocial Factors and Short-term Pain and Functional Recovery After Hip Arthroscopy for Femoroacetabular Impingement Syndrome.

J Athl Train 2021 Oct;56(10):1064-1071

University of Kentucky, Lexington.

Context: Low mental health scores, depression, and anxiety are associated with worse pain and functional outcomes after hip arthroscopy for patients with femoroacetabular impingement syndrome (FAIS). Preoperative screening of psychosocial factors such as self-efficacy, kinesiophobia, and pain catastrophizing may further aid in identifying patients at risk for poorer outcomes.

Objective: To compare preoperative function and psychosocial factors between patients with and those without elevated postoperative pain 3 months after hip arthroscopy for FAIS. Read More

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October 2021

Psychometric evaluation of the Danish language version of the field practice experiences questionnaire for students in teacher education (FPE-DK) using item analysis according to the Rasch model.

Authors:
Tine Nielsen

PLoS One 2021 18;16(10):e0258459. Epub 2021 Oct 18.

Department of Applied Research in Education and Social Sciences, UCL University College [UCL Erhvervsakademi og Professionshoejskole], Odense, Denmark.

The aim of the study was to conduct a first validation of three field practice experience scales intended to measure students' opportunities to learn through observation of other teachers, own practice and feedback on own practice of 12 key teaching activities while in field practice placement as part of teacher education programs. The scales were translated and adapted from the elementary teaching candidate survey from the Development of Ambitious Instruction project. Items were adapted to refer to the teaching subject students were training in, and the response scale was modified. Read More

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October 2021

Patient Satisfaction With Remote Consultations in a Primary Care Setting.

Cureus 2021 Sep 8;13(9):e17814. Epub 2021 Sep 8.

Faculty of Health Sciences, University of Bristol, Bristol, GBR.

Introduction In recent years, the use of remote consultations has increased considerably. Many patient encounters in general practice are now conducted by phone or computer as opposed to traditional face-to-face appointments. The aim of this study was to measure patient satisfaction with remote consultations in a primary care setting. Read More

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September 2021

Relationship Between Brain Volumes and Objective Balance and Gait Measures in Parkinson's Disease.

J Parkinsons Dis 2021 Oct 13. Epub 2021 Oct 13.

Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA.

Background: Instrumented measures of balance and gait measure more specific balance and gait impairments than clinical rating scales. No prior studies have used objective balance/gait measures to examine associations with ventricular and brain volumes in people with Parkinson's disease (PD).

Objective: To test the hypothesis that larger ventricular and smaller cortical and subcortical volumes are associated with impaired balance and gait in people with PD. Read More

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October 2021

Gait asymmetry is associated with performance-based physical function among adults with lower-limb amputation.

Physiother Theory Pract 2021 Oct 17:1-11. Epub 2021 Oct 17.

Delaware Limb Loss Studies, Department of Physical Therapy, University of Delaware, Newark DE, USA.

Background: Adults with lower-limb amputation walk with an asymmetrical gait and exhibit poor functional outcomes, which may negatively impact quality-of-life.

Objective: To evaluate associations between gait asymmetry and performance-based physical function among adults with lower-limb amputation.

Methods: A cross-sectional study involving 38 adults with a unilateral transtibial (N = 24; 62. Read More

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October 2021

A systematic, concept-based method of developing the exposure measure for drug safety and effectiveness studies.

Pharmacoepidemiol Drug Saf 2021 Oct 17. Epub 2021 Oct 17.

Collaboration for Outcomes Research and Evaluation (CORE), Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada.

Purpose: In drug safety and effectiveness studies based on secondary data, the choice of an appropriate exposure measure for a given outcome can be challenging. Different measures of exposure can yield different estimates of treatment effect and safety. There is a knowledge gap with respect to developing and refining measures of drug exposure, to ensure that the exposure measure addresses the study question and is suitable for statistical analysis. Read More

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October 2021

Going round in circles - the Papez Circuit in Alzheimer's Disease.

Eur J Neurosci 2021 Oct 16. Epub 2021 Oct 16.

Norwich Medical School, University of East Anglia, Norwich, UK.

The hippocampus is regarded as the pivotal structure for episodic memory symptoms associated with Alzheimer's disease (AD) pathophysiology. However, what is often overlooked is that the hippocampus is 'only' one part of a network of memory critical regions, the Papez circuit. Other Papez circuit regions are often regarded as less relevant for AD as they are thought to sit 'downstream' of the hippocampus. Read More

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October 2021

Recovery Home Environment Characteristics Associated with Recovery Capital.

J Drug Issues 2020 Apr 11;51(2):253-267. Epub 2020 Dec 11.

Alcohol Research Group, Public Health Institute, Emeryville, California.

Background: Recovery capital refers to internal and external resources that facilitate recovery from alcohol and drug disorders. Examples include support from friends and family, access to health and other services, stable housing and finances, and internal assets, such as self-esteem and motivation. Recovery capital is receiving increased emphasis as an integral component of addiction services. Read More

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[Comparative study on radio-opacity of dental composite resin materials'determination using film imaging and digital imaging].

Authors:
Y Li H Lin T J Zhang

Beijing Da Xue Xue Bao Yi Xue Ban 2021 Oct;53(5):995-1001

Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & NMPA Key Laboratory for Dental Materials, Beijing 100081, China.

Objective: To compare the effects of different imaging methods on radio-opacity. Digital imaging and traditional film imaging were used to determine the radio-opacity of dental composite resin materials.

Methods: Fourteen dental composite resins commonly used in clinic were prepared for disc samples with thickness of 1 mm and diameter of 15 mm respectively. Read More

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October 2021

Does it pay to go green? The environmental innovation effect on corporate financial performance.

J Environ Manage 2021 Dec 24;300:113695. Epub 2021 Sep 24.

University of Tunis, ISG-T, LR GEF-2A, Tunis, Tunisia.

The current global economy demands synergies between ecological responsiveness and business models. To analyse this dynamic, this study investigates the relationship between green innovation and corporate financial performance for German HDAX companies from 2008 to 2019 by constructing an green innovation measure. A two-step GMM system and penalised-spline estimation are used to test the linear relationship between green innovation and financial proxies (return on assets, return on invested capital, and the market-to-book ratio). Read More

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December 2021

Phenotypic association among performance, feed efficiency and methane emission traits in Nellore cattle.

PLoS One 2021 14;16(10):e0257964. Epub 2021 Oct 14.

Institute of Animal Science, Beef Cattle Research Center, Sertãozinho, SP, Brazil.

Enteric methane (CH4) emissions are a natural process in ruminants and can result in up to 12% of energy losses. Hence, decreasing enteric CH4 production constitutes an important step towards improving the feed efficiency of Brazilian cattle herds. The aim of this study was to evaluate the relationship between performance, residual feed intake (RFI), and enteric CH4 emission in growing Nellore cattle (Bos indicus). Read More

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October 2021

Development and Proof of Concept of an Audit Toolkit for the Safe Handling of Cytotoxic Drugs in Low- and Middle-Income Countries.

JCO Glob Oncol 2021 Sep;7:1480-1489

Pharmacy, Geneva University Hospitals, Geneva, Switzerland.

Purpose: Chemotherapies are considered high-risk drugs for patient and staff safety. Considering the rising burden of cancer and the increasing use of chemotherapy drugs in low- and middle-income countries (LMICs), promoting continuous improvements in the safety and quality of practices in these settings is essential. This paper describes the development and proof of concept of a toolkit to audit chemotherapy handling practices in the health care facilities of LMICs. Read More

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September 2021

Kinomics platform using GBM tissue identifies BTK as being associated with higher patient survival.

Life Sci Alliance 2021 Dec 13;4(12). Epub 2021 Oct 13.

Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK

Better understanding of GBM signalling networks in-vivo would help develop more physiologically relevant ex vivo models to support therapeutic discovery. A "functional proteomics" screen was undertaken to measure the specific activity of a set of protein kinases in a two-step cell-free biochemical assay to define dominant kinase activities to identify potentially novel drug targets that may have been overlooked in studies interrogating GBM-derived cell lines. A dominant kinase activity derived from the tumour tissue, but not patient-derived GBM stem-like cell lines, was Bruton tyrosine kinase (BTK). Read More

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December 2021

Supporting Self-Directed Learning: Development of a Faculty Evaluation Scale.

Teach Learn Med 2021 Oct 13:1-10. Epub 2021 Oct 13.

Department of Science Education, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York, USA.

We sought to assess medical education faculty members' ability to support students in the development of self-regulated learning skills. It is broadly agreed that medical students should become self-directed lifelong learners to succeed as physicians. To that end, many instruments have been developed that measure student attributes of self-directed learning (SDL). Read More

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October 2021