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A ROS-responsive fluorescent probe detecting experimental colitis by functional polymeric nanoparticles.

Int J Pharm 2021 Sep 21:121125. Epub 2021 Sep 21.

Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, Shanghai 200237, China; Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China; Department of Pharmaceutics, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Current evidence shows that oxidative stress plays an essential role in the pathogenesis and progression of inflammatory bowel disease (IBD). TotalROX (λabs/λem = 425/525 nm) is a ratiometric probe with high detection sensitivity and a superior capacity to monitor total cellular oxidative capacity. Herein, we investigated the potential of combining totalROX with an oral nanoparticle delivery system to detect the degree of colitis. Read More

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September 2021

Sulforaphane-Dependent Up-Regulation of NRF2 Activity Alleviates Both Systemic Inflammatory Response and Lung Injury After Hemorrhagic Shock/Resuscitation In Mice.

Shock 2021 Sep 23. Epub 2021 Sep 23.

Department of Orthopedics, Trauma and Reconstructive Surgery, University Hospital RWTH Aachen, Aachen/Germany Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany Department of Bone and Joint Surgery, The First Affiliated Hospital of Shandong First Medical University, Jinan City, Shandong province, P R China.

Abstract: Hemorrhagic shock/resuscitation (HS/R) is closely associated with overwhelming oxidative stress and systemic inflammation. As an effective activator of the nuclear factor-erythroid factor 2 related factor 2 (Nrf2) pathway, sulforaphane (SFN) exerts antioxidant and anti-inflammatory effects. We explored SFN's effects on alveolar macrophages (AMs), systemic inflammation, and pulmonary damage in an isolated murine HS/R model. Read More

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September 2021

NUAK2 silencing inhibits the proliferation, migration and epithelial‑to‑mesenchymal transition of cervical cancer cells via upregulating CYFIP2.

Mol Med Rep 2021 Nov 24;24(5). Epub 2021 Sep 24.

Department of Obstetrics and Gynecology, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430010, P.R. China.

NUAK family kinase 2 (NUAK2) has been reported to be involved in various cancer cell processes, including proliferation, apoptosis and invasion, by targeting multiple genes. However, to the best of our knowledge, its biological function in cervical cancer (CC) has not yet been elucidated. Therefore, the present study aimed to measure the expression of NUAK2 and to evaluate its functions in CC. Read More

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November 2021

B-Cell Immunophenotyping to Predict Vaccination Outcome in the Immunocompromised - A Systematic Review.

Front Immunol 2021 7;12:690328. Epub 2021 Sep 7.

Department of Immunology, Leiden University Medical Center (LUMC), Leiden, Netherlands.

Vaccination is the most effective measure to prevent infections in the general population. Its efficiency strongly depends on the function and composition of the immune system. If the immune system lacks critical components, patients will not be fully protected despite a completed vaccination schedule. Read More

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September 2021

Serum lncRNA-ANRIL and SOX9 expression levels in glioma patients and their relationship with poor prognosis.

World J Surg Oncol 2021 Sep 23;19(1):287. Epub 2021 Sep 23.

Department of Neurosurgery, Guangrao County People's Hospital, No. 180 Huayuan Road, Dongying, Guangrao County, 257300, P.R. China.

Background: lncRNA-CDKN2B antisense RNA 1 (ANRIL) and SRY-box transcription factor 9 (SOX9) has abnormal expression in many tumors including glioma, but the underlying molecular mechanism is unclear. This study set out to investigate the serum lncRNA-ANRIL and SOX9 levels in glioma patients and their effects on prognosis.

Methods: We enrolled 142 glioma patients admitted to our hospital from May 2014 to May 2016 into the research group (RG) and 120 healthy subjects receiving concurrent physical examinations into the control group (CG). Read More

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September 2021

Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure.

Ecotoxicol Environ Saf 2021 Sep 21;225:112796. Epub 2021 Sep 21.

Department of Environmental Health, School of Public Health, Zhengzhou University, Environment and Health Innovation Team, Zhengzhou, Henan 450001, PR China. Electronic address:

To identify the role of the Hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure. Environmental response genes (ERGs) of bone injury induced by fluoride exposure were obtained from the Comparative Toxicogenomics Database, and annotated by STRING for KEGG pathway enrichment analysis. The CCK-8 kit was used to measure the proliferation of ATDC5 cells. Read More

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September 2021

Adherence is a key factor for interpreting the results of exercise interventions.

Physiotherapy 2021 Sep 20;113:8-11. Epub 2021 Sep 20.

School of Kinesiology, Faculty of Health Sciences, University of Western Ontario, London, N6A 3K7, Canada.

Adherence may be an important factor to consider when interpreting the results of an exercise intervention. This article will discuss the importance of measuring and reporting adherence to exercise interventions that are designed to improve targeted health outcomes. Additionally, we will propose and describe a universal method to measure and report adherence to exercise interventions. Read More

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September 2021

Axonal growth on surfaces with periodic geometrical patterns.

PLoS One 2021 23;16(9):e0257659. Epub 2021 Sep 23.

Department of Physics and Astronomy, Tufts University, Medford, Massachusetts, United States of America.

The formation of neuron networks is a complex phenomenon of fundamental importance for understanding the development of the nervous system, and for creating novel bioinspired materials for tissue engineering and neuronal repair. The basic process underlying the network formation is axonal growth, a process involving the extension of axons from the cell body towards target neurons. Axonal growth is guided by environmental stimuli that include intercellular interactions, biochemical cues, and the mechanical and geometrical features of the growth substrate. Read More

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September 2021

Real-Time Monitoring of Changes in Cardiac Contractility Using Silicon Cantilever Arrays Integrated with Strain Sensors.

ACS Sens 2021 Sep 23. Epub 2021 Sep 23.

MEMS and Nanotechnology Laboratory, School of Mechanical Systems Engineering, Chonnam National University, Gwangju 61186, Korea.

This paper proposes the use of sensor-integrated silicon cantilever arrays to measure drug-induced cardiac toxicity in real time. The proposed cantilever sensors, unlike the conventional electrophysiological methods, aim to evaluate cardiac toxicity by measuring the contraction force of the cardiomyocytes corresponding to the target drugs. The surface of the silicon cantilever consists of microgrooves to maximize the alignment and the contraction force of the cardiomyocytes. Read More

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September 2021

Extracorporeal membrane oxygenation in Jehovah's Witness patients: Case report, literature review, and summary of recommendations.

Perfusion 2021 Sep 23:2676591211047774. Epub 2021 Sep 23.

Intensive Care Medicine Department, Vall d'Hebron University Hospital, Barcelona, Spain.

Extracorporeal Membrane Oxygenation (ECMO) is commonly associated with a high blood transfusion requirement. Jehovah's Witness patients present a particular challenge. The impossibility of transfusing blood cells and starting anticoagulation treatment are common contraindications for this supportive measure. Read More

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September 2021

[Restatment. Biological management of diabetes for patients with hemoglobinopathy].

Rev Prat 2021 Jun;71(6):653-658

Biologistes médicaux, laboratoire Laborizon Maine Anjou - Biogroup, Le Mans, France.

For a better biological management of diabetes for patients with hemoglobinopathy. Glycated hemoglobin, HbA1c, as a cumulative retrospective marker of glycemic control in diabetic patients could be faulty in patients with hemoglobinopathy. Abnormal hemoglobin could introduce bias analytically during HbA1c measure but also due to its physiopathology by altering the lifespan of red blood cells and/or the normal glycation process. Read More

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Consensus-based clustering of single cells by reconstructing cell-to-cell dissimilarity.

Brief Bioinform 2021 Sep 21. Epub 2021 Sep 21.

School of Mathematics and Statistics at Shandong University, Weihai, China.

The development of single-cell ribonucleic acid (RNA) sequencing (scRNA-seq) technology has led to great opportunities for the identification of heterogeneous cell types in complex tissues. Clustering algorithms are of great importance to effectively identify different cell types. In addition, the definition of the distance between each two cells is a critical step for most clustering algorithms. Read More

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September 2021

Short Assay Design for Micronucleus Detection in Human Lymphocytes.

Biomed Res Int 2021 11;2021:2322257. Epub 2021 Sep 11.

Applied Science, Antonio Nariño University, Bogotá, Colombia.

There has been a constant need to develop new and faster cytogenetic assays to measure the instability induced by genotoxic agents in the field of cytogenetic research, an example of which is the micronucleus assay. Micronuclei are fragments or complete chromosomes that remain in the cytoplasm during mitosis. With their high sensitivity and specificity detection, their presence can indicate environmental and occupational genotoxic effects. Read More

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September 2021

Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces.

Front Cell Dev Biol 2021 6;9:706126. Epub 2021 Sep 6.

Advanced Imaging Center, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United States.

The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface. Consequently, many force measurement techniques rely on optical microscopy to measure forces being applied by cells on their environment, to visualize specimen deformations due to external forces, or even to directly apply a physical perturbation to the sample via photoablation or optogenetic tools. Recent developments in advanced microscopy offer improved approaches to enhance spatiotemporal resolution, imaging depth, and sample viability. Read More

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September 2021

Association between masticatory muscle activity and oral conditions in young female college students.

Anat Cell Biol 2021 Sep 23. Epub 2021 Sep 23.

Department of Dental Hygiene, Division of Health Sciences, Dongseo University, Busan, Korea.

The purpose of this study was to determine the characteristics of masticatory muscle activity and various oral condition factors, and to analyze their associations in order to improve the prevention and diagnosis of masticatory muscle-related diseases. This study included 30 Korean females (mean age of 20 years, age range of 19-21 years). Participants were instructed to complete a self-written questionnaire on factors that may affect their muscle activity. Read More

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September 2021

Transdermal vaccination via 3D-printed microneedles induces potent humoral and cellular immunity.

Proc Natl Acad Sci U S A 2021 09;118(39)

Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;

Vaccination is an essential public health measure for infectious disease prevention. The exposure of the immune system to vaccine formulations with the appropriate kinetics is critical for inducing protective immunity. In this work, faceted microneedle arrays were designed and fabricated utilizing a three-dimensional (3D)-printing technique called continuous liquid interface production (CLIP). Read More

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September 2021

FoXA2 promotes esophageal squamous cell carcinoma progression by ZEB2 activation.

World J Surg Oncol 2021 Sep 22;19(1):286. Epub 2021 Sep 22.

Department of Radiation Oncology, Tianjin Medical University Second Hospital, No. 23, Pingjiang Road, Hexi District, Tianjin, 300211, China.

Background: It has been reported that Forkhead transcription family member (FOXA2) regulates esophageal squamous cell carcinoma (ESCC) progression. However, the specific mechanism, by which FOXA2 promotes ESCC malignant progression, remains unclear.

Materials And Methods: QRT-PCR and western blotting were applied to measure FOXA2 expression in ESCC tissues, while CCK-8 assay and Transwell assays were used to investigate the effect of FOXA2 on ESCC. Read More

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September 2021

Dextran sulfate inhibits cell proliferation and induces apoptosis by regulating EZH2 in gastric carcinoma.

Curr Cancer Drug Targets 2021 Sep 22. Epub 2021 Sep 22.

Department of Gastroenterology, Henan NO.3 Provincial People's Hospital, Zhengzhou. China.

Background: Gastric cancer (GC) is one of the most common gastrointestinal malignancies. According to reports, the enhancer of zeste homolog 2 (EZH2) exhibits carcinogenic function in a variety of cancers. Therefore, EZH2 may be a potential therapeutic target for the treatment of human cancer. Read More

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September 2021

Development of a Cell-Based Biosensor for Residual Detergent Detection in Decellularized Scaffolds.

ACS Synth Biol 2021 Sep 22. Epub 2021 Sep 22.

Department of Medical Biotechnology, School of Paramedicine, Guilan University of Medical Sciences, 4256 Rasht, Iran.

Ex vivo engineering of organs that uses decellularized whole organs as a scaffold with autologous stem cells is a potential alternative to traditional transplantation. However, one of the main challenges in this approach is preparing cytocompatible scaffolds. So far, high-precision and specific evaluation methods have not been developed for this purpose. Read More

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September 2021

Use of Cocultures to Measure the Blood-Brain Barrier Permeability of Oxytocin.

Methods Mol Biol 2022 ;2384:247-255

Department of Basic Research on Social Recognition and Memory, Research Centre for Child Mental Development, Kanazawa University, Kanazawa, Japan.

Primary monkey brain capillary endothelial cell cultures, with rat pericytes and astrocytes, provide an assay system for predicting the ability of oxytocin (OT) to cross the blood-brain barrier (BBB), using a commercially available in vitro BBB kit. The integrity of the in vitro "BBB," which has a high transendothelial electrical resistance (TEER), can be established approximately 4 days after preparations for experiments. Dominant endothelial transport of OT is from the upper (luminal blood side) to lower (abluminal brain side) chambers, dose-dependently. Read More

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January 2022

Employing the Operational Model to Measure System-Independent OTR Efficacy.

Methods Mol Biol 2022 ;2384:201-220

Department of Biomedical Sciences, Macquarie University, Sydney, NSW, Australia.

Ideally, pharmacological analysis is based on quantitative measurements applied toward unveiling biological mechanisms and guiding medicinal chemistry efforts in drug discovery and basic research. The most robust analysis frameworks generate findings which can be dissociated from the specific experimental setting to provide insights of a broader scope. With insufficient efficacy being the leading cause of drug failures in late-stage clinical trials, more rigorous preclinical definitions might assist in better translation. Read More

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January 2022

DNA methylation-based signature of CD8+ tumor-infiltrating lymphocytes enables evaluation of immune response and prognosis in colorectal cancer.

J Immunother Cancer 2021 Sep;9(9)

Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

Background: Tumor-infiltrating lymphocytes (TILs), especially CD8+ TILs, can be used for predicting immunotherapy responsiveness and survival outcome. However, the evaluation of CD8+ TILs currently relies on histopathological methodology with high variability. We therefore aimed to develop a DNA methylation signature for CD8+ TILs (CD8+ MeTIL) that could evaluate immune response and prognosis in colorectal cancer (CRC). Read More

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September 2021

Hemoglobin A-Using Epidemiology to Guide Medical Practice: Kelly West Award Lecture 2020.

Authors:
Elizabeth Selvin

Diabetes Care 2021 Sep 21. Epub 2021 Sep 21.

Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD

The discovery that HbA was a valid and reliable measure of average glucose exposure was one of the most important advances in diabetes care. HbA was rapidly adopted for monitoring glucose control and is now recommended for the diagnosis of diabetes. HbA has several advantages over glucose. Read More

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September 2021

Increasing of matrix metalloproteinase 3 in bovine endometritis.

Theriogenology 2021 Sep 4;175:83-88. Epub 2021 Sep 4.

Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, 730050, PR China; Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou, 730050, PR China.

Matrix metalloproteinase 3 (MMP3), a key member of the MMPs family, is involved in the modulation of endometrial inflammation and innate immunity. However, the role of MMP3 in bovine endometritis remains unknown. To investigate the role of MMP3 in bovine endometritis, endometrial MMP3 expression were determined in uterine biopsies from twenty 40-60 days postpartum dairy cows, which six were healthy cows and fourteen were endometritic cows. Read More

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September 2021

Effects of palmitic acid and eicosapentaenoic acid on angiogenesis of porcine vascular endothelial cells.

Vet Med Sci 2021 Sep 21. Epub 2021 Sep 21.

Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou, China.

Restricted placental angiogenesis is an important cause of intrauterine growth retardation in piglets. During pregnancy, sow obesity can result in an increase in placental lipid deposition, subsequently inhibiting placental angiogenesis and fetal development. However, the effect of different types of fatty acids on placental angiogenesis is still unclear. Read More

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September 2021

Exosomal-mediated transfer of APCDD1L-AS1 induces 5-fluorouracil resistance in oral squamous cell carcinoma via miR-1224-5p/nuclear receptor binding SET domain protein 2 (NSD2) axis.

Bioengineered 2021 Dec;12(1):7188-7204

Department of Stomatology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China.

Oral squamous cell carcinoma (OSCC) poses a threat to public health worldwide. LncRNA APCDD1L-AS1 has been reported to participate in tumorigenesis and development of acquired chemoresistance. However, the role of APCDD1L-AS1 in 5-fluorouracil (5-FU) resistance regulation within OSCC is still obscure. Read More

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December 2021

Protective effects of Schisandrin B against D-GalN-induced cell apoptosis in human hepatocyte (L02) cells via modulating Bcl-2 and Bax.

Bioengineered 2021 Dec;12(1):7205-7214

School of Medicine, Tonghua Normal University, Tonghua, China.

Schisandrin B is a dibenzocyclooctadiene derivative extracted from (Turcz.) Baill., that exhibits anti-oxidation, anti-inflammation, anti-tumor and hepatoprotective activities. Read More

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December 2021

Expression and biological functions of Ancylostoma ceylanicum saposin-like protein.

Parasitol Res 2021 Sep 21. Epub 2021 Sep 21.

Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.

Ancylostoma ceylanicum is a common zoonotic nematode that inhabits the small intestine of humans, dogs, and cats. Saposin-like proteins (SLPs) have hemolytic and antibacterial activities and could be used as diagnostic or vaccine candidates. To explore the biological functions of Ancylostoma ceylanicum SLP (Ace-SLP-1), cDNA-encoding Ace-SLP-1 mature peptide was cloned into prokaryotic expression vector pET-28a and transformed into Escherichia coli BL21 (DE3) to induce expression. Read More

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September 2021

Peripheral blood mononuclear cells - Can they provide a clue to the pathogenesis of Graves' Orbitopathy?

Endocrine 2021 Sep 20. Epub 2021 Sep 20.

Department of Endocrinology and Metabolism, Institute of Post Graduate Medical Education and Research and SSKM Hospital, Kolkata, 700020, West Bengal, India.

Purpose: Graves' disease (GD) is an autoimmune disorder affecting primarily the thyroid gland. The most common extrathyroidal manifestation of GD is known as Graves' orbitopathy (GO). Bone marrow-derived fibrocytes represent a subset of monocytes in peripheral blood mononuclear cells (PBMCs), infiltrate the orbital tissues, and contribute to the pathogenesis of GO. Read More

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September 2021

Markers for Mitochondrial ROS Status.

Methods Mol Biol 2022 ;2363:199-213

Department of Plant Bioenergetics, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.

Mitochondria actively participate in oxygenic metabolism and are one of the major sources of reactive oxygen species (ROS) production in plant cells. However, instead of measuring ROS concentrations in organelles it is more worthwhile to observe active ROS generation or downstream oxidation products, because the steady state level of ROS is easily buffered. Here, we describe how to measure the in vitro production of superoxide anion radicals (O·) by mitochondria and the release of O· into the cytosol. Read More

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January 2022