27 results match your criteria mcm6 licensing

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Cell Cycle Regulatory Protein Expression in Multinucleated Giant Cells of Giant Cell Tumor of Bone: do They Proliferate?

Pathol Oncol Res 2021 3;27:643146. Epub 2021 May 3.

1 Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary.

Cells of the monocyte macrophage lineage form multinucleated giant cells (GCs) by fusion, which may express some cell cycle markers. By using a comprehensive marker set, here we looked for potential replication activities in GCs, and investigated whether these have diagnostic or clinical relevance in giant cell tumor of bone (GCTB). GC rich regions of 10 primary and 10 first recurrence GCTB cases were tested using immunohistochemistry in tissue microarrays. Read More

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Stabilisation of half MCM ring by Cdt1 during DNA insertion.

Nat Commun 2021 03 19;12(1):1746. Epub 2021 Mar 19.

Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IDIBGI), Parc Hospitalari Martí i Julià de Salt, Catalunya, Spain.

Origin licensing ensures precise once per cell cycle replication in eukaryotic cells. The Origin Recognition Complex, Cdc6 and Cdt1 load Mcm2-7 helicase (MCM) into a double hexamer, bound around duplex DNA. The complex formed by ORC-Cdc6 bound to duplex DNA (OC) recruits the MCM-Cdt1 complex into the replication origins. Read More

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Gene set enrichment analysis and meta-analysis identified 12 key genes regulating and controlling the prognosis of lung adenocarcinoma.

Oncol Lett 2019 Jun 9;17(6):5608-5618. Epub 2019 Apr 9.

Department of Thoracic Surgery, Sichuan Cancer Hospital and Research Institute, Chengdu, Sichuan 610041, P.R. China.

The aim of the present study was to analyze lung adenocarcinoma-associated microarray data and identify potentially crucial genes. The gene expression profiles were downloaded from the Gene Expression Omnibus database and 6 datasets, of which 2 were discarded and 4 were retained, were preprocessed using packages in the R computing language. Subsequently, Gene Set Enrichment Analysis (GSEA) and meta-analysis was used to screen the common pathways and differentially expressed genes at the transcriptional level. Read More

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Function of the amino-terminal region of human MCM4 in helicase activity.

J Biochem 2018 Dec;164(6):449-460

College of Science, Ibaraki University, Mito, Ibaraki, Japan.

The amino-terminal region of eukaryotic MCM4 is characteristic of the presence of a number of phosphorylation sites for CDK and DDK, suggesting that the region plays regulatory roles in the MCM2-7 helicase function. However, the roles are not fully understood. We analyzed the role of the amino-terminal region of human MCM4 by using MCM4/6/7 helicase as a model for MCM2-7 helicase. Read More

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December 2018

MCM family in HCC: MCM6 indicates adverse tumor features and poor outcomes and promotes S/G2 cell cycle progression.

BMC Cancer 2018 02 20;18(1):200. Epub 2018 Feb 20.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Minichromosome Maintenance family (MCMs), as replication licensing factors, is involved in the pathogenesis of tumors. Here, we investigated the expression of MCMs and their values in hepatocellular carcinoma (HCC).

Methods: MCMs were analyzed in 105 samples including normal livers (n = 15), cirrhotic livers (n = 40), HCC (n = 50) using quantitative polymerase chain reaction (qPCR) (Cohort 1). Read More

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February 2018

Cell cycle analysis can differentiate thin melanomas from dysplastic nevi and reveals accelerated replication in thick melanomas.

Virchows Arch 2014 May 30;464(5):603-12. Epub 2014 Mar 30.

1st Department of Pathology and Experimental Cancer Research and MTA-SE Tumor Progression Research Group, Semmelweis University, Ulloi ut 26, Budapest, 1085, Hungary.

Cell replication integrates aberrations of cell cycle regulation and diverse upstream pathways which all can contribute to melanoma development and progression. In this study, cell cycle regulatory proteins were detected in situ in benign and malignant melanocytic tumors to allow correlation of major cell cycle fractions (G1, S-G2, and G2-M) with melanoma evolution. Dysplastic nevi expressed early cell cycle markers (cyclin D1 and cyclin-dependent kinase 2; Cdk2) significantly more (p < 0. Read More

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Over expression of minichromosome maintenance genes is clinically correlated to cervical carcinogenesis.

PLoS One 2013 17;8(7):e69607. Epub 2013 Jul 17.

Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, Uttar Pradesh, India.

Minichromosome Maintenance (MCM) proteins play important roles in cell cycle progression by mediating DNA replication initiation and elongation. Among 10 MCM homologues MCM 2-7 form a hexamer and assemble to the pre-replication complex acting as replication licensing factors. Binding and function of MCM2-7 to pre-replication complex is regulated by MCM10 mediated binding of RECQL4 with MCM2-7. Read More

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An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly.

Mol Cell 2013 May 18;50(4):577-88. Epub 2013 Apr 18.

DNA Replication Group, MRC Clinical Sciences Centre, Imperial College, London W12 0NN, UK.

In Saccharomyces cerevisiae and higher eukaryotes, the loading of the replicative helicase MCM2-7 onto DNA requires the combined activities of ORC, Cdc6, and Cdt1. These proteins load MCM2-7 in an unknown way into a double hexamer around DNA. Here we show that MCM2-7 recruitment by ORC/Cdc6 is blocked by an autoinhibitory domain in the C terminus of Mcm6. Read More

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Wolbachia-induced aae-miR-12 miRNA negatively regulates the expression of MCT1 and MCM6 genes in Wolbachia-infected mosquito cell line.

PLoS One 2012 16;7(11):e50049. Epub 2012 Nov 16.

School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia.

Background: Best recognized for its role in manipulating host reproduction, the parasitic gram-negative Wolbachia pipientis is known to colonize a wide range of invertebrates. The endosymbiotic bacterium has recently been shown to cause a life-shortening effect as well as inhibiting replication of arboviruses in Aedes aegypti; although the molecular mechanisms behind these effects are largely unknown. MicroRNAs (miRNAs) have been determined to have a wide range of roles in regulating gene expression in eukaryotes. Read More

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Plant MCM proteins: role in DNA replication and beyond.

Plant Mol Biol 2011 Dec 25;77(6):537-45. Epub 2011 Oct 25.

International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.

Mini-chromosome maintenance (MCM) proteins form heterohexameric complex (MCM2-7) to serve as licensing factor for DNA replication to make sure that genomic DNA is replicated completely and accurately once during S phase in a single cell cycle. MCMs were initially identified in yeast for their role in plasmid replication or cell cycle progression. Each of six MCM contains highly conserved sequence called "MCM box", which contains two ATPase consensus Walker A and Walker B motifs. Read More

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December 2011

Promoter of a salinity and cold stress-induced MCM6 DNA helicase from pea.

Plant Signal Behav 2011 Jul;6(7):1006-8

International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

The eukaryotic hetrohexameric mini-chromosome maintenance (MCM2-7) proteins complex provides DNA unwinding function during the DNA replication. The complex also functions as DNA replication licensing factor which ensures that the DNA in genome is replicated only once per cell division cycle. Recently, a single subunit MCM6 from pea has been shown to contain helicase and ATPase activities in vitro. Read More

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The interacting domains of hCdt1 and hMcm6 involved in the chromatin loading of the MCM complex in human cells.

Cell Cycle 2010 Dec 15;9(24):4848-57. Epub 2010 Dec 15.

Division of Life Science, Center for Cancer Research, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.

The stepwise assembly of pre-replicative complexes (pre-RCs) is essential for the initiation of DNA replication. Cdt1, a component of the pre-RC, is required for the loading of the minichromosome maintenance (MCM) complex onto chromatin. Cdt1 physically interacts with the MCM complex, and this interaction mainly occurs between Cdt1 and Mcm6 in human cells. Read More

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December 2010

Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex.

Nucleic Acids Res 2010 Sep 26;38(16):5409-18. Epub 2010 Apr 26.

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.

Mcm2-7 is recruited to eukaryotic origins of DNA replication by origin recognition complex, Cdc6 and Cdt1 thereby licensing the origins. Cdc6 is essential for origin licensing during DNA replication and is readily destabilized from chromatin after Mcm2-7 loading. Here, we show that after origin licensing, deregulation of Cdc6 suppresses DNA replication in Xenopus egg extracts without the involvement of ATM/ATR-dependent checkpoint pathways. Read More

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September 2010

Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point.

BMC Cell Biol 2010 Apr 16;11:26. Epub 2010 Apr 16.

Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA.

Background: Cytometric measurements of DNA content and chromatin-bound Mcm2 have demonstrated bimodal patterns of expression in G1. These patterns, the replication licensing function of Mcm proteins, and a correlation between Mcm loading and cell cycle commitment for cells re-entering the cell cycle, led us to test the idea that cells expressing a defined high level of chromatin-bound Mcm6 in G1 are committed--i.e. Read More

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Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.

Cell 2009 Nov 5;139(4):719-30. Epub 2009 Nov 5.

Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms EN6 3LD, UK.

The licensing of eukaryotic DNA replication origins, which ensures once-per-cell-cycle replication, involves the loading of six related minichromosome maintenance proteins (Mcm2-7) into prereplicative complexes (pre-RCs). Mcm2-7 forms the core of the replicative DNA helicase, which is inactive in the pre-RC. The loading of Mcm2-7 onto DNA requires the origin recognition complex (ORC), Cdc6, and Cdt1, and depends on ATP. Read More

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November 2009

Biochemical characterization of fragmented human MCM2.

FEBS J 2008 Feb 11;275(4):727-38. Epub 2008 Jan 11.

Mitsubishi Kagaku Institute of Life Sciences (MITILS), Machida, Tokyo, Japan.

The molecular dissection of human MCM2, a constituent of MCM2-7 licensing factor complex, was performed to identify the region responsible for its biochemical activities. Partial digestion with trypsin dissected the MCM2 protein into a central region (148-676) containing ATPase motifs and a C-terminal region (677-895). These two fragments, along with three other fragments (148-441, 442-676 and 442-895), were produced using the wheat germ cell-free system and were examined for their ability to inhibit MCM4/6/7 helicase activity. Read More

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February 2008

Accumulation and dynamics of proteins of the MCM family during mouse oogenesis and the first embryonic cell cycle.

Int J Dev Biol 2007 ;51(4):283-95

Department of Embryology, Institute of Zoology, Warsaw University, Warsaw, Poland.

We describe the localization of three proteins of the minichromosome maintenance (MCM) family, Mcm2, -6 and -7 in mouse ovarian oocytes. We showed that Mcm proteins are stored in two forms: soluble and insoluble. Soluble Mcm2, -6 and -7 were uniformly distributed in the nuclei of ovarian oocytes. Read More

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October 2007

Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.

Exp Cell Res 2006 Oct 13;312(17):3360-9. Epub 2006 Jul 13.

Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK.

Mcm2-7 proteins are generally considered to function as a heterohexameric complex, providing helicase activity for the elongation step of DNA replication. These proteins are loaded onto replication origins in M-G1 phase in a process termed licensing or pre-replicative complex formation. It is likely that Mcm2-7 proteins are loaded onto chromatin simultaneously as a pre-formed hexamer although some studies suggest that subcomplexes are recruited sequentially. Read More

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October 2006

Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development.

J Biol Chem 2005 May 4;280(20):19689-94. Epub 2005 Apr 4.

Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Wako, Saitama, Japan.

Cdt1 is an essential component for the assembly of a pre-replicative complex. Cdt1 activity is inhibited by geminin, which also participates in neural development and embryonic differentiation in many eukaryotes. Although Cdt1 homologues have been identified in organisms ranging from yeast to human, geminin homologues had not been described for Caenorhabditis elegans and fungi. Read More

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Human cytomegalovirus prevents replication licensing by inhibiting MCM loading onto chromatin.

EMBO Rep 2003 Jan;4(1):42-6

Department of Pediatrics, Laboratory for Molecular Biology, Charité, CCM-Ziegelstrasse, 5-9, Humboldt University, 10098 Berlin, Germany.

To allow DNA replication only once per cell cycle, origins of replication are reactivated ('licensed') during each G1 phase. Licensing is facilitated by assembly of the pre-replicative complex (pre-RC) at origins that concludes with loading the mini-chromosome maintenance (MCM) complex onto chromatin. Here we show that a virus exploits pre-RC assembly to selectively inhibit cellular DNA replication. Read More

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January 2003

Drosophila minichromosome maintenance 6 is required for chorion gene amplification and genomic replication.

Mol Biol Cell 2002 Feb;13(2):607-20

Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Duplication of the eukaryotic genome initiates from multiple origins of DNA replication whose activity is coordinated with the cell cycle. We have been studying the origins of DNA replication that control amplification of eggshell (chorion) genes during Drosophila oogenesis. Mutation of genes required for amplification results in a thin eggshell phenotype, allowing a genetic dissection of origin regulation. Read More

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February 2002

Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2.

J Biol Chem 2000 May;275(21):16235-41

Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.

A strong body of evidence indicates that cyclin-dependent protein kinases are required not only for the initiation of DNA replication but also for preventing over-replication in eukaryotic cells. Mcm proteins are one of the components of the replication licensing system that permits only a single round of DNA replication per cell cycle. It has been reported that Mcm proteins are phosphorylated by the cyclin-dependent kinases in vivo, suggesting that these two factors are cooperatively involved in the regulation of DNA replication. Read More

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Stepwise regulated chromatin assembly of MCM2-7 proteins.

J Biol Chem 2000 Mar;275(12):8426-31

Institute of Human Genetics, CNRS, Genome Dynamics and Development, 141 rue de la Cardonille, Montpellier, Cedex 5, France.

Acquisition of the competence to replicate requires the assembly of the MCM2-7 (minichromosome maintenance) protein complex onto pre-replicative chromatin, a step of the licensing reaction. This step is thought to occur through binding of a heterohexameric MCM complex containing the six related MCM subunits. Here we show that assembly of the MCM complex onto pre-replicative chromatin occurs through sequential stabilization of specific MCM subunits. Read More

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cDNA cloning and expression during development of Drosophila melanogaster MCM3, MCM6 and MCM7.

Gene 1998 Sep;217(1-2):177-85

Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-8681, Japan.

cDNAs encoding three Drosophila melanogaster MCM proteins, DmMCM3, DmMCM6 and DmMCM7, candidates of DNA replication-licensing factors, were cloned and sequenced. The deduced amino-acid sequences displayed 60, 59 and 68% identities with the respective Xenopus laevis homologues, XMCM3, XMCM6 and XMCM7. Six members of the D. Read More

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September 1998

HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5.

Genes Cells 1997 Jun;2(6):381-99

Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, Suita City, Japan.

Background: The tight regulatory mechanism that prevents more than one round of chromosomal DNA replication per cell cycle is thought to require the function of Mcm/P1 proteins. We report here the structural and functional analyses of HsMcm6, a human homologue of the Mis5 of Schizosaccharomyces pombe.

Results: We demonstrate here that the transcription of the HsMCM6 gene was repressed in quiescent cells but was rapidly induced at the G1/S phase by growth factor stimulation. Read More

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Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21.

FEBS Lett 1996 Dec;398(2-3):135-40

MRC Human Biochemical Genetics Unit, Wolfson House (UCL), London, UK.

Four exons of a human homologue of a yeast cell division cycle gene (MCM6/mis5, which is thought to encode a DNA replication licensing factor) have been identified 3.3 kb upstream from the start of transcription of the intestinal lactase gene on human chromosome 2q21, initially by similarity to a rat 'intestinal crypt-cell replication factor'. RT-PCR analysis shows, that unlike lactase, MCM6 is not restricted in its tissue distribution and does not show person-to-person variation in the level of expression in adult intestine. Read More

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December 1996

Mouse MCM proteins: complex formation and transportation to the nucleus.

Genes Cells 1996 Nov;1(11):977-93

Research Centre for Molecular Genetics, Hokkaido University, Kita, Sapporo, Japan.

Background: The members of the MCM protein family, including MCM2, MCM3, Cdc21, CDC46, Mis5 and CDC47, are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes. They bind to chromatin during G1 and detach from it during S phase as if they license the chromatin to replicate. However, unlike the originally proposed 'licensing factor' and the budding yeast homologues, mammalian MCM2 and P1MCM3 proteins appeared to be localized in the nucleus during the interphase. Read More

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November 1996
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