105 results match your criteria mcf‑7 cells

The comparison between light-scattering detectors based on LED and photodiode for immunoprecipitation assays of transferrin and ferritin.

Anal Chim Acta 2021 Aug 11;1175:338753. Epub 2021 Jun 11.

University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093, Warsaw, Poland.

Undoubtedly, light-emitting diodes (LEDs) and photodiodes (PDs) are indispensable optoelectronic devices in modern analytical chemistry. LEDs can serve as either light emitters or detectors, thus being an alternative to the most popular detection systems consisted of PD. In this contribution, a comparison between LED-LED and LED-PD detectors, operating in turbidimetric and nephelometric modes, has been carried out for immunoprecipitation detection of transferrin and ferritin. Read More

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Decoy bypass for appetite suppression in obese adults: role of synergistic nutrient sensing receptors GPR84 and FFAR4 on colonic endocrine cells.

Gut 2021 Jun 3. Epub 2021 Jun 3.

Centre for Neuroscience, Surgery and Trauma, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.

Objective: Colonic enteroendocrine cells (EECs) store and release potent anorectic hormones that are key regulators of satiety. EECs express multiple nutrient sensing receptors, particularly for medium-chain fatty acids (MCFAs): GPR84 and FFAR4. Here we show a non-surgical approach with targeted colonic delivery of MCFA, which induces EEC and neuronal activation leading to anorectic effects. Read More

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Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation.

Methods Mol Biol 2021 ;2277:125-132

University of Giessen and Marburg Lung Center (UGMLC), Justus-Liebig- University of Giessen, Giessen, Germany.

Mitochondrial dysfunction is regarded as a key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The influence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under inflammatory conditions has thus far not been investigated. In the following protocol we describe the assessment of mitochondrial respiration using high-resolution respirometry under inflammatory and baseline conditions. Read More

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Medium-chain acyl CoA dehydrogenase protects mitochondria from lipid peroxidation in glioblastoma.

Cancer Discov 2021 May 26. Epub 2021 May 26.

Genomic Medicine, The University of Texas MD Anderson Cancer Center

Glioblastoma (GBM) is highly resistant to chemo- and immune-based therapies and targeted inhibitors. To identify novel drug targets, we screened orthotopically implanted, patient-derived glioblastoma sphere-forming cells (GSCs) using an RNAi library to probe essential tumor cell metabolic programs. This identified high dependence on mitochondrial fatty acid metabolism. Read More

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The medium-chain fatty acid decanoic acid reduces oxidative stress levels in neuroblastoma cells.

Sci Rep 2021 03 17;11(1):6135. Epub 2021 Mar 17.

Biosciences Zoology/Physiology-Neurobiology, ZHMB (Center of Human and Molecular Biology) Faculty NT-Natural Science and Technology, Saarland University, 66123, Saarbrücken, Germany.

Enhanced oxidative stress is a contributing factor in the pathogenesis of several neurodegenerative disorders such as Alzheimer´s disease. Beneficial effects have been demonstrated for medium-chain fatty acids (MCFAs) nutritionally administered as medium-chain triglycerides (MCTs) or coconut oil (CO). The observed effects on cognitive impairment are generally attributed to the hepatic metabolism of MCFAs, where resulting ketone bodies serve as an alternate energy source to compensate for the impaired glucose utilisation in the human brain. Read More

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Microbial-derived antigens and metabolites in spondyloarthritis.

Semin Immunopathol 2021 04 10;43(2):163-172. Epub 2021 Feb 10.

Department of Pathology, NYU School of Medicine, 522 First Ave. Smilow Research Bldg 511, New York, NY, 10016, USA.

Spondyloarthritis (SpA) is a group of chronic, immune-mediated, inflammatory diseases affecting the bone, synovium, and enthesis. Microbiome, the community of microorganisms that has co-evolved with human hosts, plays a pivotal role in human health and disease. This invisible "essential organ" supplies the host with a myriad of chemicals and molecules. Read More

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Hepatic synthesis of triacylglycerols containing medium-chain fatty acids is dominated by diacylglycerol acyltransferase 1 and efficiently inhibited by etomoxir.

Mol Metab 2021 03 23;45:101150. Epub 2020 Dec 23.

LIMES Life and Medical Sciences Institute, Rheinische Friedrich-Wilhelms-Universität Bonn, Carl-Troll-Str. 31, 53115 Bonn, Germany. Electronic address:

Objective: Medium-chain fatty acids (MCFAs) play an increasing role in human nutrition. In the liver, one fraction is used for synthesis of MCFA-containing triacylglycerol (MCFA-TG), and the rest is used for oxidative energy production or ketogenesis. We investigated which enzymes catalyse the synthesis of MCFA-TG and how inhibition of MCFA-TG synthesis or fatty acid (FA) oxidation influences the metabolic fate of the MCFAs. Read More

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Lauric acid alleviates insulin resistance by improving mitochondrial biogenesis in THP-1 macrophages.

Mol Biol Rep 2020 Dec 1;47(12):9595-9607. Epub 2020 Dec 1.

Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900, Kampar, Perak, Malaysia.

Mitochondrial dysfunction plays a crucial role in the central pathogenesis of insulin resistance and type 2 diabetes mellitus. Macrophages play important roles in the pathogenesis of insulin resistance. Lauric acid is a 12-carbon medium chain fatty acid (MCFA) found abundantly in coconut oil or palm kernel oil and it comes with multiple beneficial effects. Read More

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December 2020

Dynamic Metabolome Analysis Reveals the Metabolic Fate of Medium-Chain Fatty Acids in AML12 Cells.

J Agric Food Chem 2020 Oct 19;68(43):11997-12010. Epub 2020 Oct 19.

Department of Systems Life Sciences, Graduate School of Systems Life Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

Several studies in hepatocyte cell lines reported that medium-chain fatty acids (MCFAs) with 6-12 carbons showed different metabolic properties from long-chain fatty acids (LCFAs). However, these studies reported unclear effects of different fatty acid molecules on hepatocyte metabolism. This study is aimed to capture the metabolic kinetics of MCFA assimilation in AML12 cells treated with octanoic acid (FA 8:0), decanoic acid (FA 10:0), or lauric acid (FA12:0) [LCFA; oleic acid (FA 18:1)] metabolic profiling and dynamic metabolome analysis with C-labeling. Read More

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October 2020

20 Years an Orphan: Is GPR84 a Plausible Medium-Chain Fatty Acid-Sensing Receptor?

DNA Cell Biol 2020 Nov 1;39(11):1926-1937. Epub 2020 Oct 1.

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.

GPR84 is an inflammation-induced receptor highly expressed on immune cells, yet its endogenous ligand is still unknown. This makes any interpretation of its physiological activity difficult. However, experiments with potent synthetic agonists have highlighted what the receptor can do, namely, enhance proinflammatory signaling and macrophage effector functions such as phagocytosis. Read More

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November 2020

Reactivation of fatty acid oxidation by medium chain fatty acid prevents myocyte hypertrophy in H9c2 cell line.

Mol Cell Biochem 2021 Jan 30;476(1):483-491. Epub 2020 Sep 30.

Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, 695 011, India.

Metabolic shift is an important contributory factor for progression of hypertension-induced left ventricular hypertrophy into cardiac failure. Under hypertrophic conditions, heart switches its substrate preference from fatty acid to glucose. Prolonged dependence on glucose for energy production has adverse cardiovascular consequences. Read More

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January 2021

Decanoic Acid and Not Octanoic Acid Stimulates Fatty Acid Synthesis in U87MG Glioblastoma Cells: A Metabolomics Study.

Front Neurosci 2020 23;14:783. Epub 2020 Jul 23.

Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.

Medium-chain fatty acids (MCFA) are dietary components with a chain length ranging from 6 to 12 carbon atoms. MCFA can cross the blood-brain barrier and in the brain can be oxidized through mitochondrial β-oxidation. As components of ketogenic diets, MCFA have demonstrated beneficial effects on different brain diseases, such as traumatic brain injury, Alzheimer's disease, drug-resistant epilepsy, diabetes, and cancer. Read More

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Alterations in Circulating Fatty Acid Are Associated With Gut Microbiota Dysbiosis and Inflammation in Multiple Sclerosis.

Front Immunol 2020 7;11:1390. Epub 2020 Jul 7.

IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.

Butyric acid (BA) is a short-chain fatty acid (SCFA) with anti-inflammatory properties, which promotes intestinal barrier function. Medium-chain fatty acids (MCFA), including caproic acid (CA), promote TH1 and TH17 differentiation, thus supporting inflammation. Since most SCFAs are absorbed in the cecum and colon, the measurement of BA in peripheral blood could provide information on the health status of the intestinal ecosystem. Read More

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Effects of medium- and long-chain fatty acids on acetaminophen- or rifampicin-induced hepatocellular injury.

Food Sci Nutr 2020 Jul 19;8(7):3590-3601. Epub 2020 May 19.

State Key Laboratory of Food Science and Technology Nanchang University Nanchang China.

Drug-induced liver injury (DILI) is one of the common adverse effects of drug therapy, which is closely associated with oxidative stress, apoptosis, and inflammation response. Medium-chain fatty acids (MCFA) were reported to relieve inflammation and attenuate oxidative stress. However, little has been known about the hepatoprotective effects of MCFA in DILI. Read More

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Structure-guided reshaping of the acyl binding pocket of 'TesA thioesterase enhances octanoic acid production in E. coli.

Metab Eng 2020 09 24;61:24-32. Epub 2020 Apr 24.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Medium-chain fatty acids (C6-C10) have attracted much attention recently for their unique properties compared to their long-chain counterparts, including low melting points and relatively higher carbon conversion yield. Thioesterase enzymes, which can catalyze the hydrolysis of acyl-ACP (acyl carrier protein) to release free fatty acids (FAs), regulate both overall FA yields and acyl chain length distributions in bacterial and yeast fermentation cultures. These enzymes typically prefer longer chain substrates. Read More

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September 2020

The Medium-Chain Fatty Acid Receptor GPR84 Mediates Myeloid Cell Infiltration Promoting Steatohepatitis and Fibrosis.

J Clin Med 2020 Apr 16;9(4). Epub 2020 Apr 16.

Department of Hepatology & Gastroenterology, Charité University Medicine Berlin, 13353 Berlin, Germany.

Medium-chain fatty acids (MCFAs) have been associated with anti-steatotic effects in hepatocytes. Expression of the MCFA receptor GPR84 (G protein-coupled receptor 84) is induced in immune cells under inflammatory conditions and can promote fibrogenesis. We aimed at deciphering the role of GPR84 in the pathogenesis of non-alcoholic steatohepatitis (NASH), exploring its potential as a therapeutic target. Read More

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Multidimensional Metabolic Engineering for Constructing Efficient Cell Factories.

Jianhui Liu Jin Hou

Trends Biotechnol 2020 05 19;38(5):468-469. Epub 2020 Mar 19.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, PR China. Electronic address:

Microbial production of medium-chain fatty acids (MCFAs) is challenging, as they are rare metabolites and toxic to cells. Recently, Zhu et al. performed multidimensional engineering in Saccharomyces cerevisiae, including engineering fatty acid synthases and improving cellular tolerance to MCFAs, to build an efficient MCFA cell factory. Read More

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Effects of glycerol-esters of saturated short- and medium chain fatty acids on immune, health and growth variables in veal calves.

Prev Vet Med 2020 May 5;178:104983. Epub 2020 Apr 5.

Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium.

In veal and dairy beef production systems, Holstein bull calves experience many stressors and excessive pathogen exposure, necessitating the use of antimicrobials for welfare and production reasons. The aim of this randomized clinical trial was to explore the effects of esterified fatty acids used as feed supplement on health, production and immune variables in veal calves. Different glycerol-esters of fatty acids were used: short chain fatty acid (SCFA)-based glycerol-mono- (C4) and tributyrate (C4), and medium chain fatty acid (MCFA)-based glycerol-monocaprylate/monocaprinate (C8/C10) and glycerol-monolaurate (C12) in two different doses. Read More

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Effects of dietary fatty acids on gut health and function of pigs pre- and post-weaning.

J Anim Sci 2020 Apr;98(4)

Department of Animal Science, Faculty of Technical Sciences, Aarhus University, Tjele, Denmark.

Fatty acids (FA) play a major role in relation to mucosal immune responses, epithelial barrier functions, oxidative stress, and inflammatory reactions. The dietary FA composition and the molecular structures (chain length and number of double bonds) influence digestion, absorption and metabolism, and the bioactivity of the FA. Piglets post-weaning having an immature intestine and not fully formed immune functions are very vulnerable to invading microorganisms. Read More

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A Neural basis for Octanoic acid regulation of energy balance.

Mol Metab 2020 04 9;34:54-71. Epub 2020 Jan 9.

Department of Physiology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, 3010, VIC, Australia. Electronic address:

Objectives: Nutrient sensing by hypothalamic neurons is critical for the regulation of food intake and energy expenditure. We aimed to identify long- and medium-chain fatty acid species transported into the brain, their effects on energy balance, and the mechanisms by which they regulate activity of hypothalamic neurons.

Methods: Simultaneous blood and cerebrospinal fluid (CSF) sampling was undertaken in rats and metabolic analyses using radiolabeled fatty acid tracers were performed on mice. Read More

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Effects of Medium Chain Fatty Acids on Intestinal Health of Monogastric Animals.

Curr Protein Pept Sci 2020 ;21(8):777-784

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China

Medium-chain fatty acids (MCFAs) are the main form of Medium Chain Triglycerides (MCTs) utilized by monogastric animals. MCFAs can be directly absorbed and supply rapid energy to promote the renewal and repair of intestinal epithelial cells, maintain the integrity of intestinal mucosal barrier function, and reduce inflammation and stress. In our review, we pay more attention to the role of MCFAs on intestinal microbiota and mucosa immunity to explore MCFA's positive effect. Read More

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Evaluating Strategies to Reduce Ruminal Protozoa and Their Impacts on Nutrient Utilization and Animal Performance in Ruminants - A Meta-Analysis.

Front Microbiol 2019 15;10:2648. Epub 2019 Nov 15.

Department of Animal Sciences, University of Florida, Gainesville, FL, United States.

Several studies have evaluated the effects of complete or partial ruminal protozoa (RP) inhibition; however, to this date, no practical suppressant has been identified and used in large scale. This meta-analysis quantitatively evaluates the effectiveness of multiple strategies on inhibiting RP numbers and their influence on ruminal fermentation and animal performance. This study compared 66 peer-reviewed articles (16 manuscripts for complete and 50 manuscripts for partial RP inhibition that used supplemental phytochemicals and lipids, published from 2000 to 2018, to inhibit RP . Read More

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November 2019

Binding to medium and long chain fatty acyls is a common property of HEAT and ARM repeat modules.

Sci Rep 2019 10 2;9(1):14226. Epub 2019 Oct 2.

Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Covalent post-translational modification (PTM) of proteins with acyl groups of various carbon chain-lengths regulates diverse biological processes ranging from chromatin dynamics to subcellular localization. While the YEATS domain has been found to be a prominent reader of acetylation and other short acyl modifications, whether additional acyl-lysine reader domains exist, particularly for longer carbon chains, is unclear. Here, we employed a quantitative proteomic approach using various modified peptide baits to identify reader proteins of various acyl modifications. Read More

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October 2019

Regulation of mitochondrial metabolism in murine skeletal muscle by the medium-chain fatty acid receptor Gpr84.

FASEB J 2019 11 15;33(11):12264-12276. Epub 2019 Aug 15.

Department of Pharmacology, School of Medical Sciences, University of New South Wales (UNSW) Sydney, Sydney, New South Wales, Australia.

Fatty acid receptors have been recognized as important players in glycaemic control. This study is the first to describe a role for the medium-chain fatty acid (MCFA) receptor G-protein-coupled receptor (Gpr) 84 in skeletal muscle mitochondrial function and insulin secretion. We are able to show that Gpr84 is highly expressed in skeletal muscle and adipose tissue. Read More

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November 2019

Effects of a feed additive blend on broilers challenged with heat stress.

Avian Pathol 2019 Dec 7;48(6):582-601. Epub 2019 Aug 7.

Institute for Risk Assessment Sciences, Division of Veterinary Pharmacy, Pharmacotherapy and Toxicology, Faculty of Veterinary Medicine, Utrecht University , Utrecht , Netherlands.

We evaluated a blend of medium-chain fatty acids (MCFA), organic acids, and a polyphenol antioxidant on gut integrity. Eighty Ross Broilers were exposed to 20-22°C (control - normothermic) or to 35-39.5°C (heat stress) for eight hours a day for a period of 1 or 5 days. Read More

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December 2019

Chemical-nutritional characteristics and aromatic profile of milk and related dairy products obtained from goats fed with extruded linseed.

Asian-Australas J Anim Sci 2019 Apr 15:148-156. Epub 2019 Apr 15.

Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.

Objective: This study aimed to evaluate the effect of dietary integration with extruded linseed (EL) on fatty acid (FA) and aromatic profile of goat cheese after 60 (T60) days of ripening.

Methods: Thirty goats were divided in two groups. The control group (CG) was fed with conventional diet, whereas the experimental group (EL+) was fed with conventional diet supplemented with 10% of EL. Read More

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Synthesis and characterization of cyclohexane-1R,2R-diamine-based Pt(iv) dicarboxylato anticancer prodrugs: their selective activity against human colon cancer cell lines.

Dalton Trans 2019 Jan;48(2):435-445

Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Viale Michel 11, 15121 Alessandria, Italy.

Three pairs of asymmetric dicarboxylato derivatives based on the cisplatin and oxaliplatin-like skeletons have been synthesized de novo or re-synthesized. The axial ligands consist of one medium-chain fatty acid (MCFA), namely clofibrate (i.e. Read More

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January 2019

Improving medium chain fatty acid production in Escherichia coli by multiple transporter engineering.

Food Chem 2019 Jan 23;272:628-634. Epub 2018 Aug 23.

College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

Medium-chain fatty acids (C-C, MCFAs) represent valuable molecules for food and pharmaceutical industries. However, MCFAs in Escherichia coli induce stresses and toxicity, reducing final yields. These phenotypes could be alleviated if MCFA export from cells was accelerated, yet information on proteins responsible for exporting MCFAs remains limited. Read More

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January 2019

Hexanoic, Octanoic and Decanoic Acids Promote Basal and Insulin-Induced Phosphorylation of the Akt-mTOR Axis and a Balanced Lipid Metabolism in the HepG2 Hepatoma Cell Line.

Molecules 2018 Sep 11;23(9). Epub 2018 Sep 11.

Molecular Metabolism of Lipids Laboratory, BioMed Research Center, Biological Sciences Department, University of Quebec in Montreal (UQAM), Montreal, QC H3C 3P8, Canada.

Metabolic illnesses such as non-alcoholic fatty liver disease (NAFLD) are in constant increase worldwide. Highly consumed long chain fatty acids (LCFA) are among the most obesogenic and steatogenic nutrients. Hepatic steatosis is associated with several complications such as insulin resistance. Read More

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September 2018

d-Polyarginine Lipopeptides as Intestinal Permeation Enhancers.

ChemMedChem 2018 10 20;13(19):2045-2052. Epub 2018 Aug 20.

Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 10, 08028, Barcelona, Spain.

An estimated 285 million people were living with diabetes in 2010, and this number is expected to reach 440 million by 2030. Current treatment of this disease involves the intradermal injection of insulin analogues. Many alternative administration routes have been proposed, the oral route being the most widely studied. Read More

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October 2018