Genomics Proteomics Bioinformatics 2021 Mar 10. Epub 2021 Mar 10.
Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary; Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged H-6726, Hungary. Electronic address:
Single-cell mass cytometry (SCMC) combines features of traditional flow cytometry (FACS) with mass spectrometry, making it possible to measure several parameters at the single-cell level for a complex analysis of biological regulatory mechanisms. In this study, weoptimizedSCMC to analyze hemocytes of the Drosophila innate immune system. We used metal-conjugated antibodies (H2, H3, H18, L1, L4, and P1 at the cell surface, intracellular 3A5 and L2) and anti-IgM (L6 at the cell surface) to detect the levels of antigens, while anti-GFP was used to detect crystal cells in the immune induced samples. Read More