26,986 results match your criteria matrix influence

Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium.

Arch Oral Biol 2021 Apr 24;127:105134. Epub 2021 Apr 24.

Department of Physiology and Pathology, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP, Brazil.

Objective: This study aimed to assess the influence of the bisphosphonates zoledronic acid and sodium alendronate on MMP-2 and MMP-9 synthesis and activity by gingival fibroblasts seeded onto titanium substrate.

Design: Titanium discs were placed in 24-well cell culture plates and gingival fibroblasts were seeded (1 × 10 cells/discs) on them using Dulbecco's Modified Eagle's Medium (DMEM) + 10 % fetal bovine serum (FBS) for 24 h. After this period, a fresh serum-free DMEM containing zoledronic acid or sodium alendronate at 0. Read More

View Article and Full-Text PDF

Diurnal evolutions and sources of water-soluble chromophoric aerosols over Xi'an during haze event, in Northwest China.

Sci Total Environ 2021 Apr 29;786:147412. Epub 2021 Apr 29.

School of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.

Atmospheric brown carbon and their chemical behavior potentially impacts the climate and air quality. Due to lack of researches on the atmospheric chromophores by using online experimental instrument, so using the offline EEM approaches to study their types, sources and chemical processes. In this study, PILS-EEM-TOC system (Particle into liquid sampler coupled with excitation-emission matrix and total organic carbon) was developed in order to distinguish the hourly evolutions and sources of water-soluble chromophoric organic matters in atmospheric fine particles. Read More

View Article and Full-Text PDF

UVC-induced degradation of cilastatin in natural water and treated wastewater.

Chemosphere 2021 Apr 27;280:130668. Epub 2021 Apr 27.

Laboratory of Aquatic Chemistry, School of Environmental Engineering, Technical University of Crete, GR-73100, Chania, Crete, Greece. Electronic address:

This work reports for the first time the UVC photodegradation of cilastatin, a renal dehydropeptidase inhibitor co-adminstered with the imipenem antibiotic. Initially, solutions of cilastatin at varying concentrations were prepared in ultra-pure water and the direct photolysis of cilastatin was monitored under 254-nm irradiation. Degradation was slower at higher initial cilastatin concentrations, due to absorption saturation. Read More

View Article and Full-Text PDF

Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.

PLoS One 2021 7;16(5):e0246358. Epub 2021 May 7.

Department of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, United States of America.

Nephrocystin (NPHP1) is a ciliary transition zone protein and its ablation causes nephronophthisis (NPHP) with partially penetrant retinal dystrophy. However, the precise requirements of NPHP1 in photoreceptors are not well understood. Here, we characterize retinal degeneration in a mouse model of NPHP1 and show that NPHP1 is required to prevent infiltration of inner segment plasma membrane proteins into the outer segment during the photoreceptor maturation. Read More

View Article and Full-Text PDF

MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting AP1-mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis.

Cell Death Dis 2021 May 6;12(5):451. Epub 2021 May 6.

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Metastasis-associated protein 2 (MTA2) is a transcription factor that is highly associated with matrix metalloproteinase 12 (MMP12). Thus, we hypothesized that MTA2 may regulate MMP12 expression and is involved in cervical cancer metastasis. Results showed that MTA2 and MMP12 were highly expressed in cervical cancer cells, and MTA2 knockdown reduced MMP12 expression and inhibited the metastasis of cervical cancer cells in xenograft mice. Read More

View Article and Full-Text PDF

Determination of tetrabromobisphenol-A/S and their eight derivatives in abiotic (soil/dust) samples using ultra-high performance liquid chromatography-tandem mass spectrometry.

J Chromatogr A 2021 Apr 20;1647:462152. Epub 2021 Apr 20.

State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Center for Excellence in Deep Earth Science, Guangzhou, 510640, China.

Tetrabromobisphenol-A and Tetrabromobisphenol-S (TBBPA/S) and their derivatives have attracted widespread attention owing to their environmental risks and adverse effects on human health. In this study, we developed an analytical method based on ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-MS/MS) for the simultaneous determination of TBBPA/S and their eight derivatives in soil samples. After ultrasonic extraction, TBBPA/S and their derivatives were purified using an LC-Si cartridge with 1 mL acetone and 30 mL dichloromethane/n-hexane (1/1, v/v) as the eluent. Read More

View Article and Full-Text PDF

SATB1 protein is associated with the epithelial‑mesenchymal transition process in non‑small cell lung cancers.

Oncol Rep 2021 Jun 6;45(6). Epub 2021 May 6.

Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50‑368 Wroclaw, Poland.

Lung cancer is one of the most frequently diagnosed neoplasms and the leading cause of cancer‑related mortality worldwide. Its predominant subtype is non‑small cell lung cancer (NSCLC), which accounts for over 80% of the cases. Surprisingly, the majority of lung cancer‑related deaths are caused not by a primary tumour itself, but by its metastasis to distant organs. Read More

View Article and Full-Text PDF

Reduced kinase D‑interacting substrate of 220 kDa (Kidins220) in pancreatic cancer promotes EGFR/ERK signalling and disease progression.

Int J Oncol 2021 Jun 6;58(6). Epub 2021 May 6.

Cardiff China Medical Research Collaborative, Division of Cancer and Genetics, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.

Kidins220 is a transmembrane scaffold protein involved in several types of cancer. The aim of the present study was to examine the role of Kidins220 in tumorigenesis and disease progression of pancreatic cancer. The relevant signalling pathways including EGFR, EMT, and MMP were also investigated. Read More

View Article and Full-Text PDF

Cuticle Collagen Expression Is Regulated in Response to Environmental Stimuli by the GATA Transcription Factor ELT-3 in Caenorhabditis elegans.

Genetics 2020 Jun;215(2):483-495

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada L8S 4K1.

Mesbahi et al. find that environmental factors, including diet, starvation, and population density can differentially influence the penetrance of collagen mutant phenotypes. Factors that decrease the penetrance of rolling in dominant. Read More

View Article and Full-Text PDF

Antibacterial composite coatings of MgB powders embedded in PVP matrix.

Sci Rep 2021 May 5;11(1):9591. Epub 2021 May 5.

Faculty of Biology and The Research Institute of the University of Bucharest (ICUB), University of Bucharest, Splaiul Independentei 91-95, Bucharest, Romania.

Three commercial powders of MgB were tested in vitro by MTS and LDH cytotoxicity tests on the HS27 dermal cell line. Depending on powders, the toxicity concentrations were established in the range of 8.3-33. Read More

View Article and Full-Text PDF

Affinity Enrichment Chemoproteomics for Target Deconvolution and Selectivity Profiling.

Methods Mol Biol 2021 ;2228:237-252

Cellzome GmbH, GlaxoSmithKline, Heidelberg, Germany.

In order to understand the full mechanism of action of candidate drug molecules, it is critical to thoroughly characterize their interactions with endogenously expressed pharmacological targets and potentially undesired off-targets. Here we describe a chemoproteomics approach that is based on functionalized analogs of the compound of interest to affinity enrich target proteins from cell or tissue extracts. Experiments are designed as competition binding assays where free parental compound is spiked at a range of concentrations into the extracts to compete specific binders off the immobilized compound matrix. Read More

View Article and Full-Text PDF
January 2021

A semi dynamic digestion study of milk protein concentrate dispersions structured with different polysaccharides.

Curr Res Food Sci 2021 6;4:250-261. Epub 2021 Apr 6.

Department of Food Science, CiFood Multidisciplinary Center, Aarhus University, 48 Agro Food Park, Aarhus N, 8200, Denmark.

Hydrocolloids are often added as functional ingredients in foods, to better design the structure of the matrix and ensure food quality and optimal sensory properties. However, much less is known about their influence on the physical and chemical changes during gastric digestion. In this study, semi-continuous gastric digestion was applied on a model food system, prepared with milk protein concentrate (MPC) (3% w/v) and 1% alginate, pectin, guar gum, as well as a 1:1 mixture of alginate and pectin. Read More

View Article and Full-Text PDF

Expansion of hay production and marketing in Brazil.

Heliyon 2021 Apr 16;7(4):e06787. Epub 2021 Apr 16.

Department of Animal Science, State University of West Paraná, Marechal Cândido Rondon, PR, 85960-000, Brazil.

In the last decade, advances in Brazilian hay production showed that the country has the potential to produce bulky dehydrated fodder. For many years, the Brazilian hay production scientific knowledge had been based on temperate climate species, even though the best hay material are tropical grasses, as Tifton 85 Bermudagrass. Researches that focused on the comprehension of yield systems, biochemical processes, physiology, composition, and nutritional quality of tropical species under dehydration and conservation have become important to hay yield in Brazil. Read More

View Article and Full-Text PDF

Super-resolved visualization of single DNA-based tension sensors in cell adhesion.

Nat Commun 2021 05 4;12(1):2510. Epub 2021 May 4.

Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.

Cell-extracellular matrix sensing plays a crucial role in cellular behavior and leads to the formation of a macromolecular protein complex called the focal adhesion. Despite their importance in cellular decision making, relatively little is known about cell-matrix interactions and the intracellular transduction of an initial ligand-receptor binding event on the single-molecule level. Here, we combine cRGD-ligand-decorated DNA tension sensors with DNA-PAINT super-resolution microscopy to study the mechanical engagement of single integrin receptors and the downstream influence on actin bundling. Read More

View Article and Full-Text PDF

Factors that influence clinical efficacy of live biotherapeutic products.

Eur J Med Res 2021 May 4;26(1):40. Epub 2021 May 4.

KidZ Health Castle, University Hospital Brussel, Brussels Health Campus, UZ Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium.

Traditional probiotics are increasingly being used in a medical context. The use of these products as drugs is considerably different from the traditional use as food or food supplements, as, obviously, the target population is different (diseased versus healthy or at risk population). Besides the target population, also the regulatory context is different, mainly with respect to production, administration regime and type of clinical studies required. Read More

View Article and Full-Text PDF

Effect of Nitrogen Ion Implantation on the Cavitation Erosion Resistance and Cobalt-Based Solid Solution Phase Transformations of HIPed Stellite 6.

Materials (Basel) 2021 Apr 29;14(9). Epub 2021 Apr 29.

Institute of Physics, Maria Curie-Sklodowska University in Lublin, pl. M. Curie-Sklodowskiej 1, 20-031 Lublin, Poland.

From the wide range of engineering materials traditional Stellite 6 (cobalt alloy) exhibits excellent resistance to cavitation erosion (CE). Nonetheless, the influence of ion implantation of cobalt alloys on the CE behaviour has not been completely clarified by the literature. Thus, this work investigates the effect of nitrogen ion implantation (NII) of HIPed Stellite 6 on the improvement of resistance to CE. Read More

View Article and Full-Text PDF

Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro.

Biomolecules 2021 Apr 30;11(5). Epub 2021 Apr 30.

Department of Management, Information and Production Engineering, University of Bergamo, 24044 Dalmine (BG), Italy.

Cell's microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of lung epithelial adenocarcinoma (A549) cells. Read More

View Article and Full-Text PDF

Inhomogeneity of stiffness and density of the extracellular matrix within the leukoplakia of human oral mucosa as potential physicochemical factors leading to carcinogenesis.

Transl Oncol 2021 May 1;14(7):101105. Epub 2021 May 1.

Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland; Department of Microbiology and Immunology, Institute of Medical Science, Collegium Medicum, Jan Kochanowski University in Kielce, PL-25317 Kielce, Poland. Electronic address:

Oral leukoplakia is a clinical term relating to various morphological lesions, including squamous cell hyperplasia, dysplasia and carcinoma. Leukoplakia morphologically manifested as hyperplasia with epithelial dysplasia is clinically treated as precancerous condition. Nevertheless, there is a lack of good markers indicating the transformation of premalignancies towards cancer. Read More

View Article and Full-Text PDF

Homogeneous triple-phase interfaces enabling one-pot route to metal compound/carbon composites.

J Colloid Interface Sci 2021 Apr 16;599:271-279. Epub 2021 Apr 16.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou, China. Electronic address:

Metal compounds (e.g., metal phosphides/sulfides/selenides) coupled with carbon materials have recently drawn great attraction for boosting the electrochemical performances because of their appealing synergistic effect and valuable structural stability. Read More

View Article and Full-Text PDF

Development of a maleic acid-based material to selectively solid-phase extract basic compounds from environmental samples.

J Chromatogr A 2021 Apr 21;1647:462165. Epub 2021 Apr 21.

Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Marcel•lí Domingo, 1, Campus Sescelades, 43007 Tarragona, Spain.

This study presents a novel mixed-mode weak cation-exchange (WCX) material. This material was prepared by means of the functionalization of a mesoporous divinylbenzene (DVB) resin with maleic acid (maleic acid-DVB), which yielded a high carboxylic moiety content resulting in WCX interactions as well as suitable specific surface area for reversed-phase interactions. After the optimization of the solid-phase extraction (SPE) protocol to enhance the selectivity of the sorbent, this material was evaluated as a novel WCX sorbent in the SPE of a group of drugs from environmental water samples. Read More

View Article and Full-Text PDF

Uremic endothelial-derived extracellular vesicles: mechanisms of formation and their role in cell adhesion, cell migration, inflammation, and oxidative stress.

Toxicol Lett 2021 May 1. Epub 2021 May 1.

Experimental Nephrology Laboratory, Basic Pathology Department, Universidade Federal do Paraná, 81.531-980, Curitiba, PR, Brazil. Electronic address:

p-Cresyl sulfate (PCS), indoxyl sulfate (IS), and inorganic phosphate (Pi) are uremic toxins found in chronic kidney disease (CKD) that are closely related to endothelial extracellular vesicles (EVs) formation. The present study aimed to understand the role of EVs and their role in cell adhesion and migration, inflammation, and oxidative stress. Human endothelial cells were treated with PCS, IS, and Pi in pre-established uremic and kinetic recommendations. Read More

View Article and Full-Text PDF

Sex, subtext, text: Freud, Dora and the suggestive text.

Adele Tutter

Int J Psychoanal 2020 Jun 2;101(3):523-548. Epub 2020 Jun 2.

Department of Psychiatry, Vagelos School of Medicine, Columbia University, New York, NY, US.

The intertextual analysis of illuminates an aspect of the cultural matrix that informed Freud's theory-building. Specifically, the trope of the suggestive text, a literal and symbolic agent of transgressive influence, signals an intertextual relationship between the case history and a vein of literary fiction that includes novels by some of Freud's favourite authors: Cervantes (), Flaubert () and Zola (). It is posited that the suggestive text in acts both as an literal agent of dangerous suggestion, and as a figurative symbol of the occult literary influence that intrudes upon the text, impacting Freud's formulation of his subject; his documentation of her case; and his ensuing conceptualization of the transference. Read More

View Article and Full-Text PDF

Calcyclin (S100A6) Attenuates Inflammatory Response and Mediates Apoptosis of Chondrocytes in Osteoarthritis via the PI3K/AKT Pathway.

Orthop Surg 2021 May 4. Epub 2021 May 4.

Institute of Orthopaedics, Tianjin Hospital, Tianjin, China.

Objective: To clarify the regulatory effect of Calcyclin (S100A6) on chondrocytes apoptosis and its relationship with progression of osteoarthritis in an effort to explore potential therapeutic targets for osteoarthritis.

Method: Immunofluorescence assay was produced to identify the rat chondrocyte sample and western blots assay was detected the expression changes of S100A6 between control group and osteoarthritis model which induced by interleukin-1β. Adenovirus were transfected into the chondrocytes in vitro, in order to regulate the S100A6 expression. Read More

View Article and Full-Text PDF

2,5TT genotype of the MMP-9-1562C/T polymorphism may be a risk factor for thrombolytic therapy-induced hemorrhagic complications after acute ischemic stroke.

Pharmacotherapy 2021 May 4. Epub 2021 May 4.

St. Sava Hospital, Belgrade, Serbia.

Introduction: Levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) influence recombinant tissue plasminogen activator (rtPA) therapy response in patients with acute ischemic stroke (AIS). Serum levels of MMPs and TIMPs along with the expression of genes coding these proteins are related to the recovery and appearance of adverse effects (AE) after AIS. Consequently, it is important to explore whether polymorphisms in regulatory sequences of MMPs and TIMPs are associated with rtPA response in AIS patients. Read More

View Article and Full-Text PDF

cell culture model of human nasal-associated lymphoid tissue (NALT) to evaluate the humoral immune response to SARS-CoV-2 spike proteins.

Saudi J Biol Sci 2021 Apr 24. Epub 2021 Apr 24.

Otorhinolaryngology, Head& Neck Surgery Department, Faculty of Medicine, Taibah University, Madinah, Saudi Arabia.

To date, coronavirus disease 2019 (COVID-19) continues to be considered a pandemic worldwide, with a mild to severe disease presentation that is sometimes associated with serious complications that are concerning to global health authorities. Scientists are working hard to understand the pathogenicity of this novel virus, and a great deal of attention and effort has been focused on identifying therapeutics and vaccines to control this pandemic.

Methods: This study used tonsils removed from twelve patients who underwent an elective tonsillectomy in the ear, nose, and throat (ENT) department at Saudi Germany Hospital, Madinah, Saudi Arabia. Read More

View Article and Full-Text PDF

Deep learning-based downscaling of tropospheric nitrogen dioxide using ground-level and satellite observations.

Manzhu Yu Qian Liu

Sci Total Environ 2021 Jun 6;773:145145. Epub 2021 Feb 6.

NSF Spatiotemporal Innovation Center, Department of Geography and GeoInformation Science, George Mason University, USA.

Air quality is one of the major issues within an urban area that affect people's living environment and health conditions. Existing observations are not adequate to provide a spatiotemporally comprehensive air quality information for vulnerable populations to plan ahead. Launched in 2017, TROPOspheric Monitoring Instrument (TROPOMI) provides a high spatial resolution (~5 km) tropospheric air quality measurement that captures the spatial variability of air pollution, but still limited by its daily overpass in the temporal dimension and relatively short historical records. Read More

View Article and Full-Text PDF

The effect of dough mixing speed and work input on the structure, digestibility and celiac immunogenicity of the gluten macropolymer within bread.

Food Chem 2021 Apr 20;359:129841. Epub 2021 Apr 20.

School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand; Department of Chemical and Materials Engineering University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address:

Modern high-speed mechanical dough development (MDD) alters the gluten macropolymer's (GMP) structure. Changes to both the protein and food matrix structure can influence protein digestibility and immunogenicity. This study investigated the relationship between protein structural changes imparted by MDD and gluten's digestibility plus celiac reactivity. Read More

View Article and Full-Text PDF

Synthesis, biological, and photophysical studies of molecular rotor-based fluorescent inhibitors of the trypanosome alternative oxidase.

Eur J Med Chem 2021 Apr 16;220:113470. Epub 2021 Apr 16.

Instituto de Química Médica, IQM-CSIC, Juan de la Cierva 3, E-28006, Madrid, Spain. Electronic address:

We have recently reported on the development and trypanocidal activity of a class of inhibitors of Trypanosome Alternative Oxidase (TAO) that are targeted to the mitochondrial matrix by coupling to lipophilic cations via C14 linkers to enable optimal interaction with the enzyme's active site. This strategy resulted in a much-enhanced anti-parasite effect, which we ascribed to the greater accumulation of the compound at the location of the target protein, i.e. Read More

View Article and Full-Text PDF

A Programmable Toolkit to Dynamically Signal Cells Using Peptide Strand Displacement.

ACS Appl Mater Interfaces 2021 May 3. Epub 2021 May 3.

Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

The native extracellular matrix communicates and interacts with cells by dynamically displaying signals to control their behavior. Mimicking this dynamic environment is essential in order to unravel how cell-matrix interactions guide cell fate. Here, we present a synthetic platform for the temporal display of cell-adhesive signals using coiled-coil peptides. Read More

View Article and Full-Text PDF

Event-triggering control scheme for discrete time Cyberphysical Systems in the presence of simultaneous hybrid stochastic attacks.

ISA Trans 2021 Apr 27. Epub 2021 Apr 27.

Computer Engineering Department, King Fahd University of Petroleum and Minerals, P.O. Box 1350, Dhahran 31261, Saudi Arabia. Electronic address:

Industry Revolution 4.0 pushes the industry to digitize all its operations. Cyberphysical Systems (CPSs), such as autonomous automobile systems and medical monitoring are examples of this revolution. Read More

View Article and Full-Text PDF