909 results match your criteria matrix cellularity


Differential remodelling of visceral and pericardial adipose tissue in response to stress and exercise.

FASEB J 2022 May;36 Suppl 1

West Virginia University School of Medicine, University of West Virginia, Margantown, WV.

Obesity is driven by an imbalance between caloric intake and energy expenditure, causing excessive storage of nutrients in adipose tissue at different sites around the body. Increased visceral adipose tissue (VAT) is associated with diabetes, whilst pericardial adipose tissue (PAT) is associated with cardiac pathology. Adipose tissue can expand either through cellular hypertrophy or hyperplasia, with the former correlating with decreased metabolic health in obesity. Read More

View Article and Full-Text PDF

Cytomolecular Classification of Thyroid Nodules Using Fine-Needle Washes Aspiration Biopsies.

Int J Mol Sci 2022 Apr 9;23(8). Epub 2022 Apr 9.

Department of Medicine and Surgery, Pathology, University of Milano-Bicocca, 20900 Monza, Italy.

Fine-needle aspiration biopsies (FNA) represent the gold standard to exclude the malignant nature of thyroid nodules. After cytomorphology, 20-30% of cases are deemed "indeterminate for malignancy" and undergo surgery. However, after thyroidectomy, 70-80% of these nodules are benign. Read More

View Article and Full-Text PDF

Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors, and their implantation regenerated the intervertebral disc degeneration.

World J Stem Cells 2022 Feb;14(2):163-182

Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Sindh, Pakistan.

Background: Intervertebral disc degeneration (IVDD) is the leading cause of lower back pain. Disc degeneration is characterized by reduced cellularity and decreased production of extracellular matrix (ECM). Mesenchymal stem cells (MSCs) have been envisioned as a promising treatment for degenerative illnesses. Read More

View Article and Full-Text PDF
February 2022

An In Vivo Stable Isotope Labeling Method to Investigate Individual Matrix Protein Synthesis, Ribosomal Biogenesis, and Cellular Proliferation in Murine Articular Cartilage.

Function (Oxf) 2022 25;3(2):zqac008. Epub 2022 Feb 25.

Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.

Targeting chondrocyte dynamics is a strategy for slowing osteoarthritis progression during aging. We describe a stable-isotope method using in vivo deuterium oxide labeling and mass spectrometry to measure protein concentration, protein half-life, cell proliferation, and ribosomal biogenesis in a single sample of murine articular cartilage. We hypothesized that a 60-d labeling period would capture age-related declines in cartilage matrix protein content, protein synthesis rates, and cellular proliferation. Read More

View Article and Full-Text PDF
February 2022

Effects of oral losartan administration on homeostasis of articular cartilage and bone in a rabbit model.

Bone Rep 2022 Jun 28;16:101526. Epub 2022 Mar 28.

Department of Orthopaedic Surgery, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, USA.

Background And Aims: Previous work has shown that oral losartan can enhance microfracture-mediated cartilage repair in a rabbit osteochondral defect injury model. In this study, we aimed to determine whether oral losartan would have a detrimental effect on articular cartilage and bone homeostasis in the uninjured sides.

Methods: New Zealand rabbits were divided into 4 groups including normal uninjured (Normal), contralateral uninjured side of osteochondral defect (Defect), osteochondral defect plus microfracture (Microfracture) and osteochondral defect plus microfracture and losartan oral administration (10 mg/kg/day) (Losartan). Read More

View Article and Full-Text PDF

Elevated levels of active Transforming Growth Factor β1 in the subchondral bone relate spatially to cartilage loss and impaired bone quality in human knee osteoarthritis.

Osteoarthritis Cartilage 2022 Jun 21;30(6):896-907. Epub 2022 Mar 21.

Centre for Orthopaedic & Trauma Research, The University of Adelaide, Adelaide, South Australia 5000, Australia. Electronic address:

Objective: The association between the spatially distributed level of active TGFβ1 in human subchondral bone, and the characteristic structural and cellular parameters of human knee OA, was assessed.

Design: Paired subchondral bone samples from 35 OA arthroplasty patients, (15 men and 20 women, aged 69 ± 9 years) were obtained from beneath macroscopically present (CA+) or denuded cartilage (CA-) to determine the concentration of active TGFβ1 (ELISA) and its relationship to bone quality (synchrotron micro-CT), cellularity, and vascularization (histology).

Results: Bone samples beneath (CA-) regions had significantly increased concentrations of active TGFβ1 protein (mean difference: 26. Read More

View Article and Full-Text PDF

Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis.

Int J Mol Sci 2022 Mar 20;23(6). Epub 2022 Mar 20.

Unidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, Spain.

Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. Read More

View Article and Full-Text PDF

Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model.

Bioengineering (Basel) 2022 Mar 15;9(3). Epub 2022 Mar 15.

Department of Biomedical Sciences for Health, Università degli Studi di Milano, 20133 Milano, Italy.

The analysis of the morphological, structural, biochemical, and mechanical changes of the Extracellular Matrix (ECM), which occur during meniscus development, represents the goal of the present study. Medial fully developed menisci (FD, 9-month-old pigs), partially developed menisci (PD, 1-month-old piglets), and not developed menisci (ND, from stillbirths) were collected. Cellularity and glycosaminoglycans (GAGs) deposition were evaluated by ELISA, while Collagen 1 and aggrecan were investigated by immunohistochemistry and Western blot analyses in order to be compared to the biomechanical properties of traction and compression tensile forces, respectively. Read More

View Article and Full-Text PDF

Teratocarcinosarcoma-Like and Adamantinoma-Like Head and Neck Neoplasms Harboring NAB2::STAT6: Unusual Variants of Solitary Fibrous Tumor or Novel Tumor Entities?

Head Neck Pathol 2022 Mar 18. Epub 2022 Mar 18.

Department of Pathology, University of Texas Southwestern Medical Center, 6201 Harry Hines Blvd, Dallas, TX, 75390, USA.

The archetypal solitary fibrous tumor (SFT) features fibroblastic cells with varying cellularity without any particular architectural arrangement in a collagenous matrix, with staghorn vessels, CD34 and STAT6 expression, and NAB2::STAT6. To date, this fusion is thought to be specific for SFT. With more routine use of fusion gene panels, the histologic diversity of NAB2::STAT6-positive tumors is increasingly appreciated. Read More

View Article and Full-Text PDF

Research perspectives-Pipelines to human tendon transcriptomics.

J Orthop Res 2022 05 16;40(5):993-1005. Epub 2022 Mar 16.

Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Science, University of Oxford, Oxford, UK.

Tendon transcriptomics is a rapidly growing field in musculoskeletal biology. The ultimate aim of many current tendon transcriptomic studies is characterization of in vitro, ex vivo, or in vivo, healthy, and diseased tendon microenvironments to identify the underlying pathways driving human tendon pathology. The transcriptome interfaces between genomic, proteomic, and metabolomic signatures of the tendon cellular niche and the response of this niche to stimuli. Read More

View Article and Full-Text PDF

Corneal Opacity: Cell Biological Determinants of the Transition From Transparency to Transient Haze to Scarring Fibrosis, and Resolution, After Injury.

Invest Ophthalmol Vis Sci 2022 01;63(1):22

Department of Ophthalmology, University of Sao Paulo, Sao Paulo, Brazil.

Purpose: To highlight the cellular, matrix, and hydration changes associated with opacity that occurs in the corneal stroma after injury.

Methods: Review of the literature.

Results: The regulated transition of keratocytes to corneal fibroblasts and myofibroblasts, and of bone marrow-derived fibrocytes to myofibroblasts, is in large part modulated by transforming growth factor beta (TGFβ) entry into the stroma after injury to the epithelial basement membrane (EBM) and/or Descemet's membrane. Read More

View Article and Full-Text PDF
January 2022

Detection of invasive in patient blood by a fungal PCR array.

Access Microbiol 2021 3;3(12):000285. Epub 2021 Dec 3.

Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

Rare invasive fungal infections are increasingly emerging in hosts with predisposing factors such as immunodeficiency. Their timely diagnosis remains difficult, as their clinical picture may initially mimic infections with more common fungal species and species identification may be difficult with routine methods or may require time-consuming subcultures. This often results in ineffective drug administration and fatal outcomes. Read More

View Article and Full-Text PDF
December 2021

RNA isolation from micro-quantity of articular cartilage for quantitative gene expression by microarray analysis.

Int J Med Sci 2022 1;19(1):98-104. Epub 2022 Jan 1.

Division of Arthritis and Rheumatic Diseases, Oregon Health & Science University, Portland, Oregon 97239.

Isolation of quality RNA from articular cartilage has been challenging due to low cellularity and the high abundance of extracellular matrix and proteoglycan proteins. Recently developed methods for isolation of high quality RNA from cartilage are more applicable to larger cartilage specimens typically weighing at least 25 mg. While these methods generate RNA suitable for analysis, they are less successful with smaller tissue inputs. Read More

View Article and Full-Text PDF

Upregulated expression of transforming growth factor-β receptor I/II in an endemic Osteoarthropathy in China.

BMC Musculoskelet Disord 2021 Dec 20;22(1):1051. Epub 2021 Dec 20.

The Institute of Endemic Disease, School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R. China.

Background: Kashin-Beck disease (KBD) is a chronic, deforming, endemic osteochondropathy that begins in patients as young as 2-3 years of age. The pathogenesis of KBD remains unclear, although selenium (Se) deficiency and T-2 toxin food contamination are both linked to the disease. In the present study, we evaluated transforming growth factor-β receptor (TGF-βR I and II) levels in clinical samples of KBD and in pre-clinical disease models. Read More

View Article and Full-Text PDF
December 2021

Recellularization and Integration of Dense Extracellular Matrix by Percolation of Tissue Microparticles.

Adv Funct Mater 2021 Aug 23;31(35). Epub 2021 Jun 23.

Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO.

Cells embedded in the extracellular matrix of tissues play a critical role in maintaining homeostasis while promoting integration and regeneration following damage or disease. Emerging engineered biomaterials utilize decellularized extracellular matrix as a tissue-specific support structure; however, many dense, structured biomaterials unfortunately demonstrate limited formability, fail to promote cell migration, and result in limited tissue repair. Here, we developed a reinforced composite material of densely packed acellular extracellular matrix microparticles in a hydrogel, termed , that can be molded and crosslinked to mimic native tissue architecture. Read More

View Article and Full-Text PDF

Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder.

Int J Mol Sci 2021 Nov 17;22(22). Epub 2021 Nov 17.

Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.

The extracellular matrix of the bladder consists mostly of type I and III collagen, which are required during loading. During bladder injury, there is an accumulation of collagen that impairs bladder function. Little is known about the genes that regulate production of collagens in the bladder. Read More

View Article and Full-Text PDF
November 2021

The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial.

Int J Mol Sci 2021 Nov 16;22(22). Epub 2021 Nov 16.

Center for Applied NanoBioscience and Medicine, College of Medicine Phoenix, University of Arizona, 475 North 5th Street, Phoenix, AZ 85004, USA.

The decellularization of plant-based biomaterials to generate tissue-engineered substitutes or in vitro cellular models has significantly increased in recent years. These vegetal tissues can be sourced from plant leaves and stems or fruits and vegetables, making them a low-cost, accessible, and sustainable resource from which to generate three-dimensional scaffolds. Each construct is distinct, representing a wide range of architectural and mechanical properties as well as innate vasculature networks. Read More

View Article and Full-Text PDF
November 2021

The emerging PRRX1-NCOA fibroblastic neoplasm: a combined reappraisal of published tumors and two new cases.

Virchows Arch 2021 Oct 14. Epub 2021 Oct 14.

Department of Anatomic Pathology , Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123, Ta-Pei Rd., Niao-Sung District, Kaohsiung, 83301, Taiwan.

Nuclear receptor coactivator (NCOA) family gene fusions have been increasingly discovered in diverse mesenchymal neoplasms, while PRRX1-NCOA-fused fibroblastic tumors still remain insufficiently characterized. We herein present two additional PRRX1-NOCA1-positive cases sharing lobulated hypocellular growth of innocuous spindle-to-stellate cells in a fibromyxoid stroma enriched with polymorphous vessels. A constellation of low cellularity, alternating myxocollagenous matrix, bland cytomorphology, and, especially, unusual collagenous rosettes in one case were morphologically reminiscent of low-grade fibromyxoid sarcoma. Read More

View Article and Full-Text PDF
October 2021

Amniotic fluid allograft enhances the host response to ventral hernia repair using acellular dermal matrix.

J Tissue Eng Regen Med 2021 12 11;15(12):1092-1104. Epub 2021 Oct 11.

Department of Surgery, Division of Trauma, Acute, and Critical Care Surgery, Duke University Medical Center, Durham, North Carolina, USA.

Ventral hernia repair (VHR) with acellular dermal matrix (ADM) has high rates of recurrence that may be improved with allogeneic growth factor augmentation such as amniotic fluid allograft (AFA). We hypothesized that AFA would modulate the host response to improve ADM incorporation in VHR. Lewis rats underwent chronic VHR with porcine ADM alone or with AFA augmentation. Read More

View Article and Full-Text PDF
December 2021

CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4.

Int J Mol Sci 2021 Sep 8;22(18). Epub 2021 Sep 8.

Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK.

The interfascicular matrix (IFM) binds tendon fascicles and contains a population of morphologically distinct cells. However, the role of IFM-localised cell populations in tendon repair remains to be determined. The basement membrane protein laminin-α4 also localises to the IFM. Read More

View Article and Full-Text PDF
September 2021

Cell-Based Therapies for Trabecular Meshwork Regeneration to Treat Glaucoma.

Biomolecules 2021 08 24;11(9). Epub 2021 Aug 24.

Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, USA.

Glaucoma is clinically characterized by elevated intraocular pressure (IOP) that leads to retinal ganglion cell (RGC) and optic nerve damage, and eventually blindness if left untreated. Even in normal pressure glaucoma patients, a reduction of IOP is currently the only effective way to prevent blindness, by either increasing aqueous humor outflow or decreasing aqueous humor production. The trabecular meshwork (TM) and the adjacent Schlemm's canal inner wall play a key role in regulating IOP by providing resistance when aqueous humor drains through the tissue. Read More

View Article and Full-Text PDF

Focal segmental glomerulosclerosis and concurrent glomerular microangiopathy after long-term imatinib administration.

CEN Case Rep 2022 02 9;11(1):134-140. Epub 2021 Sep 9.

Division of Nephrology and Rheumatology, Department of Internal Medicine, Faculty of Medicine, Fukuoka University, Nanakuma 7-45-1, Jonan-ku, Fukuoka, 814-0180, Japan.

A 79-year-old Japanese man was admitted to our hospital because of proteinuria and kidney dysfunction. He was diagnosed with chronic myeloid leukemia 13 years before and was treated with imatinib. Deep molecular response was achieved but he developed 1+ proteinuria in the first year, which gradually worsened thereafter. Read More

View Article and Full-Text PDF
February 2022

Comparison of properties determined using electromechanical assessment (Arthro-BST™) with macroscopic and histological properties in symptomatic human articular cartilage of the hip.

Arthritis Res Ther 2021 08 31;23(1):227. Epub 2021 Aug 31.

Department of Orthopedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Bohseidai, Isehara, Kanagawa, 259-1193, Japan.

Background: Cartilage degeneration is assessed using various methods. Although macroscopic evaluation can directly measure cartilage degeneration, it cannot accurately assess cartilage properties. Histological examination is one of the most accurate methods for evaluating cartilage degeneration. Read More

View Article and Full-Text PDF

Recombinant Interleukin-19 Suppresses the Formation and Progression of Experimental Abdominal Aortic Aneurysms.

J Am Heart Assoc 2021 09 28;10(17):e022207. Epub 2021 Aug 28.

Divison of Vascular Surgery Department of Surgery Stanford University School of Medicine Stanford CA.

Background Interleukin-19 is an immunosuppressive cytokine produced by immune and nonimmune cells, but its role in abdominal aortic aneurysm (AAA) pathogenesis is not known. This study aimed to investigate interleukin-19 expression in, and influences on, the formation and progression of experimental AAAs. Methods and Results Human specimens were obtained at aneurysm repair surgery or from transplant donors. Read More

View Article and Full-Text PDF
September 2021

Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression.

Stem Cell Res Ther 2021 08 28;12(1):481. Epub 2021 Aug 28.

Department of Medical and Surgical Sciences for Children and Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy.

Introduction: Adipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Read More

View Article and Full-Text PDF

Comparison of the function and structural integrity of cryopreserved pulmonary homografts versus decellularized pulmonary homografts after 180 days implantation in the juvenile ovine model.

Cell Tissue Bank 2021 Aug 28. Epub 2021 Aug 28.

Department of Cardiothoracic Surgery, Faculty of Health Sciences, Internal Box G32), University of the Free State (UFS), P.O. Box 339, Bloemfontein, 9300, South Africa.

Homograft availability and durability remain big challenges. Increasing the post-mortem ischaemic harvesting time beyond 24 h increases the potential donor pool. Cryopreservation, routinely used to preserve homografts, damages the extracellular matrix (ECM), contributing to valve degeneration. Read More

View Article and Full-Text PDF

Deletion of Glut1 in early postnatal cartilage reprograms chondrocytes toward enhanced glutamine oxidation.

Bone Res 2021 Aug 23;9(1):38. Epub 2021 Aug 23.

Department of Orthopaedic Surgery, School of Medicine, Washington University, St. Louis, MO, USA.

Glucose metabolism is fundamental for the functions of all tissues, including cartilage. Despite the emerging evidence related to glucose metabolism in the regulation of prenatal cartilage development, little is known about the role of glucose metabolism and its biochemical basis in postnatal cartilage growth and homeostasis. We show here that genetic deletion of the glucose transporter Glut1 in postnatal cartilage impairs cell proliferation and matrix production in growth plate (GPs) but paradoxically increases cartilage remnants in the metaphysis, resulting in shortening of long bones. Read More

View Article and Full-Text PDF

PRIMUS: Comprehensive proteomics of mouse intervertebral discs that inform novel biology and relevance to human disease modelling.

Matrix Biol Plus 2021 Dec 24;12:100082. Epub 2021 Jul 24.

School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong.

Mice are commonly used to study intervertebral disc (IVD) biology and related diseases such as IVD degeneration. Discs from both the lumbar and tail regions are used. However, little is known about compartmental characteristics in the different regions, nor their relevance to the human setting, where a functional IVD unit depends on a homeostatic proteome. Read More

View Article and Full-Text PDF
December 2021

Development of a standardized histopathology scoring system for intervertebral disc degeneration and regeneration in rabbit models-An initiative of the ORSspine section.

JOR Spine 2021 Jun 3;4(2):e1147. Epub 2021 Jun 3.

Leni and Peter W. May Department of Orthopaedics Ichan School of Medicine New York New York USA.

Background: The rabbit lumbar spine is a commonly utilized model for studying intervertebral disc degeneration and for the pre-clinical evaluation of regenerative therapies. Histopathology is the foundation for which alterations to disc morphology and cellularity with degeneration, or following repair or treatment are assessed. Despite this, no standardized histology grading scale has yet been established for the spine field for any of the frequently utilized animal models. Read More

View Article and Full-Text PDF

The Effect of a Triple Combination of Bevacizumab, Sodium Hyaluronate and a Collagen Matrix Implant in a Trabeculectomy Animal Model.

Pharmaceutics 2021 Jun 17;13(6). Epub 2021 Jun 17.

Innovation, Therapy and Pharmaceutical Development in Ophthalmology (InnOftal) Research Group (UCM 920415), 28040 Madrid, Spain.

Currently available anti-scarring treatments for glaucoma filtration surgery (GFS) have potentially blinding complications, so there is a need for alternative and safer agents. The effects of the intrableb administration of a new combination of the anti-VEGF bevacizumab, sodium hyaluronate and a collagen matrix implant were investigated in a rabbit model of GFS, with the purpose of modulating inflammation, angiogenesis, fibroblast migration and fibrogenesis in the wound healing process. A comparative-effectiveness study was performed with twenty-four rabbits, randomly assigned to the following treatments: (a) biodegradable collagen matrix implant (Olo), (b) bevacizumab-loaded collagen matrix implant (Olo-BVZ), (c) bevacizumab-loaded collagen matrix implant combined with sodium hyaluronate (Olo-BVZ-H5) and (d) sham-operated animals (control). Read More

View Article and Full-Text PDF