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Exploration of the potential roles of m6A regulators in the uterus in pregnancy and infertility.

J Reprod Immunol 2021 Jun 2;146:103341. Epub 2021 Jun 2.

Laboratory of Neurobiology, College of Veterinary Medicine, China Agricultural University, Beijing, Haidian, 100193, People's Republic of China; Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, China Agricultural University, Beijing, Haidian, 100193, People's Republic of China. Electronic address:

Infertility is a prevalent female reproductive disease worldwide. Currently, there are many unknown etiologies of infertility. N6-methyladenosine (m6A) is the most prevalent modification of eukaryotic mRNA. Read More

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Tetrabromobisphenol A (TBBPA) anaerobic biodegradation occurs during acidogenesis.

Chemosphere 2021 May 26;282:130995. Epub 2021 May 26.

Laboratory of Biological Processes, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering, University of São Paulo (USP), 1100, João Dagnone Ave., Santa Angelina, Zip Code, 13563-120, São Carlos, SP, Brazil. Electronic address:

This is the first study to bring evidence on the anaerobic biodegradation of TBBPA occurring during acidogenesis in domestic sewage at environmentally relevant concentrations by complex microbial communities. This was accomplished by continuously operating two anaerobic structured bed reactors (ASTBR) for over 100 days under acidogenic (Acidogenic Reactor, AR) and multistep methanogenic (Methanogenic Reactor, MR) conditions. In the AR, the temporal carbohydrates consumption and the acetic acid production were strongly correlated with TBBPA removal by the Pearson's test. Read More

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Evaluating CO emissions from continuous flow and batch growth systems under autotrophic mode: Implications for GHG accounting of biological nutrient removal.

J Environ Manage 2021 Jun 8;294:112928. Epub 2021 Jun 8.

Department of Chemistry and Biology, Ryerson University, 350 Victoria St., Toronto, ON, M5B 2K3, Canada; Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address:

The oxidation of ammonia by autotrophic bacteria is a central part of the nitrogen cycle and a fundamental aspect of biological nutrient removal (BNR) during wastewater treatment. Autotrophic ammonia oxidation produces protons and results in net-CO production due to the neutralizing effect of bicarbonate alkalinity. Attention must be paid to the propensity for this produced CO to be transferred to the atmosphere where it can act as a greenhouse gas (GHG). Read More

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Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR).

Food Chem Toxicol 2021 Jun 8:112329. Epub 2021 Jun 8.

Flanders Research Institute for Agriculture, Fisheries and Food, ILVO, Aquatic Environment and Quality, Ankerstraat 1, B-8400, Oostende, Belgium; Department of Biology, Ghent University, Krijgslaan 281, Building S8, B-9000 Ghent, Belgium. Electronic address:

Fishery products are often subject to substitution fraud, which is hard to trace due to a lack of morphologic traits when processed, gutted, or decapitated. Traditional molecular methods (DNA barcoding) fail to identify products containing multiple species and cannot estimate original weight percentages. As a proof of concept, an Atlantic salmon (Salmo salar) specific ddPCR assay was designed to authenticate mixed food products. Read More

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Association between abdominal fat and mortality in patients undergoing cardiovascular surgery.

Ann Thorac Surg 2021 Jun 8. Epub 2021 Jun 8.

Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, South Korea. Electronic address:

Background: Obesity is associated with reduced postoperative mortality in patients undergoing cardiovascular surgery. However, body mass index cannot differentiate abdominal fat composition. This study evaluated the relationships between total abdominal, subcutaneous, and visceral fat composition and postoperative mortality in East Asian patients undergoing cardiovascular surgery. Read More

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CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer.

FEBS Open Bio 2021 Jun 11. Epub 2021 Jun 11.

Department of Urology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, China.

The development of chemoresistance reduces the efficacy of anti-cancer drugs. Cervical cancer is still one of the most common cancer types in developing countries. The oncogene HMGA2 is involved in the development and progression of tumors, but its role in chemoresistance of cervical cancer remain unclear. Read More

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A Case Report of Subclinical Myasthenia Gravis Associated with Castleman's Disease.

Am J Case Rep 2021 Jun 11;22:e930948. Epub 2021 Jun 11.

Department of Thoracic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

BACKGROUND Castleman's disease is defined as a benign lymphoproliferative disorder of uncertain origin. It is most commonly found in the area of mediastinum. Castleman's disease is classified based on pathological features into hyaline-vascular, plasma cell, and mixed variants, which the hyaline-vascular variant is the commonest in association with Myasthenia Gravis. Read More

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Kinetic disposition of diazepam and its metabolites after intravenous administration of diazepam in the horse: Relevance for doping control.

J Vet Pharmacol Ther 2021 Jun 11. Epub 2021 Jun 11.

Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne, Germany.

In horses, the benzodiazepine diazepam (DIA) is used as sedative for pre-medication or as an anxiolytic to facilitate horse examinations. As the sedative effects can also be abused for doping purposes, DIA is prohibited in equine sports. DIA is extensively metabolized to several active metabolites such as nordazepam, temazepam and oxazepam (OXA). Read More

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Nasal Lavage Fluid as a Biomedical Sample for Verification of Chlorine Exposure.

J Anal Toxicol 2021 Jun 11. Epub 2021 Jun 11.

The Swedish Defence Research Agency, CBRN Defence and Security, Cementvägen 20, Umeå, Sweden.

Chlorine is a toxic chemical that has been used as a chemical warfare agent in recent armed conflicts. There is an urgent need for methods to verify alleged uses of chlorine, and phospholipid chlorohydrins (PL-HOCl) derived from the pulmonary surfactant of exposed victims have previously been proposed as biomarkers of chlorine exposure. Here we describe an improved protocol for the chemical analysis of these biomarkers and its applicability to biomedical samples from chlorine-exposed animals. Read More

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Size-Resolved Electron Solvation in Neutral Water Clusters.

J Phys Chem A 2021 Jun 11. Epub 2021 Jun 11.

Department of Chemistry and Applied Biosciences, ETH Zurich, Vladimir-Prelog-Weg 2, CH-8093 Zurich, Switzerland.

Cluster-size-resolved ultrafast dynamics of the solvated electron in neutral water clusters with = 3 to ∼200 molecules are studied with pump-probe time-of-flight mass spectrometry after below band gap excitation. For the smallest clusters, no longer-lived (>100-200 fs) hydrated electrons were detected, indicating a minimum size of ∼ 14 for being able to sustain hydrated electrons. Larger clusters show a systematic increase of the number of hydrated electrons per molecule on the femtosecond to picosecond time scale. Read More

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Untargeted metabolomics analysis by gas chromatography/time-of-flight mass spectrometry of human serum from methamphetamine abusers.

Addict Biol 2021 Jun 10:e13062. Epub 2021 Jun 10.

School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, School of Food Science Synergetic Innovation Center of Food Safety and Nutrition, Joint International Research Laboratory on Food Safety, Jiangnan University, Wuxi, China.

Methamphetamine (METH) abuse has become a global public health problem. However, the potential mechanisms involving METH-induced metabolic disorders have thus far remained poorly understood. Metabolomics can provide a clue for the cause of apparent changes and consequently be used to investigate the METH-induced dysregulation of metabolite expression and the mechanism of metabolic disorder mechanism. Read More

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Spatial relationship between the pulmonary trunk and the left coronaries: Systematic risk assessment based on automated three-dimensional distance measurements.

Heart Rhythm O2 2020 Apr 27;1(1):14-20. Epub 2020 Apr 27.

Informatics for Life, Heidelberg, Germany.

Background: Catheter ablation of right ventricular outflow tract ventricular arrhythmias from above the pulmonary valve is being increasingly reported.

Objective: The purpose of this study was to systematically analyze the spatial relationship between the pulmonary trunk and the left coronaries.

Methods: Contrast-enhanced computed tomographic scans from 58 patients were analyzed. Read More

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Immunoglobulin G4-related masses surrounding coronary arteries: a case report.

Eur Heart J Case Rep 2021 Mar 8;5(3):ytab055. Epub 2021 Mar 8.

Department of Cardiovascular Medicine, Nara Prefecture General Medical Centre, 2-897-5, Shichijonishimachi, Nara-City, Nara 630-8581, Japan.

Background: Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is an immune-mediated fibroinflammatory condition with high serum IgG4 levels affecting various organs, such as the pancreas, lacrimal and salivary glands, thyroid, kidney, and lung. Typical cardiovascular manifestations of IgG4-RD include periaortitis, coronary arteritis, and pericarditis. However, reports of IgG4-RD associated with coronary arteritis are rare. Read More

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A case report of takotsubo syndrome complicated by ischaemic stroke: the clinical dilemma of anticoagulation.

Eur Heart J Case Rep 2021 Mar 15;5(3):ytab051. Epub 2021 Mar 15.

Cardiothoracic and Vascular Department, Cardiology Unit, University Hospital "San Giovanni di Dio e Ruggi d'Aragona", Heart Tower-Room 807, Largo Città d'Ippocrate, 84131 Salerno, Italy.

Background: Takotsubo syndrome (TTS) is an acute and transient heart failure syndrome due to reversible myocardial dysfunction characterized by a wide spectrum of possible clinical scenarios. About one-fifth of TTS patients experience adverse in-hospital events. Thromboembolic complications, especially stroke, have been reported, albeit in a minority of patients. Read More

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Physiological Characteristics and Comparative Secretome Analysis of Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates.

Front Microbiol 2021 25;12:636344. Epub 2021 May 25.

Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan, China.

Morels ( sp.) are economically important edible macro-fungi, which can grow on various synthetic or semi-synthetic media. However, the complex nutritional metabolism and requirements of these fungi remain ill-defined. Read More

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Immunotherapy using IgE or CAR T cells for cancers expressing the tumor antigen SLC3A2.

J Immunother Cancer 2021 Jun;9(6)

St John's Institute of Dermatology, School of Basic and Medical Biosciences, King's College London, London, England, UK

Background: Cancer immunotherapy with monoclonal antibodies and chimeric antigen receptor (CAR) T cell therapies can benefit from selection of new targets with high levels of tumor specificity and from early assessments of efficacy and safety to derisk potential therapies.

Methods: Employing mass spectrometry, bioinformatics, immuno-mass spectrometry and CRISPR/Cas9 we identified the target of the tumor-specific SF-25 antibody. We engineered IgE and CAR T cell immunotherapies derived from the SF-25 clone and evaluated potential for cancer therapy. Read More

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Proteomic analysis validates previous findings on wounding-responsive plant hormone signaling and primary metabolism contributing to the biosynthesis of secondary metabolites based on metabolomic analysis in harvested broccoli (Brassica oleracea L. var. italica).

Food Res Int 2021 Jul 5;145:110388. Epub 2021 May 5.

College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioesources Utilization, Ministry of Education, Dalian 116600, China.

The plant wound-response is a complex process that generates physiological modifications for protecting the wounded tissue. In this study, tandem mass tag (TMT)-based quantitative proteomic analysis was performed to clarify the comprehensive molecular mechanism for the wound-response of broccoli subjected to two wounding intensities (0.04 and 1. Read More

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Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway.

Stem Cell Res Ther 2021 Jun 10;12(1):341. Epub 2021 Jun 10.

Department of Plastic Surgery, Tangdu Hospital, Airforce Military Medical University, Xi'an, 710038, China.

Background: Epidermal stem cells (EpSCs) play a vital role in wound healing and skin renewal. Although biomaterial scaffolds have been used for transplantation of EpSCs in wound healing, the ex vivo differentiation of EpSCs limits their application.

Methods: To inhibit the differentiation of EpSCs and maintain their stemness, we developed an electrospun polycaprolactone (PCL)+cellulose acetate (CA) micro/nanofiber for the culture and transplantation of EpSCs. Read More

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Influence of the energy and digestible lysine contents of the diet on performance and egg quality traits of brown-egg laying hens from 19 to 59 weeks of age.

Poult Sci 2021 Apr 21;100(7):101211. Epub 2021 Apr 21.

Departamento de Producción Agraria, Universidad Politécnica de Madrid, 28040 Madrid, Spain. Electronic address:

The influence of nutrient density and standardized ileal digestible lys (DLys) content of the diet on egg production and egg quality traits, was studied in brown-egg laying hens from 19 to 59 wk of age. The experimental design was completely randomized with eight treatments arranged as a 2×4 factorial with two AMEn concentrations (2,680 and 2,780 kcal/kg) and four levels of DLys (0.68, 0. Read More

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Characteristics of neonicotinoid and metabolite residues in Taiwanese tea leaves.

J Sci Food Agric 2021 Jun 10. Epub 2021 Jun 10.

Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taiwan.

Background: Neonicotinoids are widely used insecticides, and tea is a popular nonalcoholic beverage in Taiwan. However, the levels of neonicotinoids in Taiwanese tea leaves remain unclear. Therefore, this study aims to understand the characteristics of neonicotinoid and metabolite residues in Taiwanese tea leaves. Read More

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An Untargeted Thermogravimetric Analysis-Fourier Transform Infrared-Gas Chromatography-Mass Spectrometry Approach for Plastic Polymer Identification.

Environ Sci Technol 2021 Jun 10. Epub 2021 Jun 10.

School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom.

Reliable chemical identification of specific polymers in environmental samples represents a major challenge in plastic research, especially with the wide range of commercial polymers available, along with variable additive mixtures. Thermogravimetric analysis-Fourier transform infrared-gas chromatography-mass spectrometry (TGA-FTIR-GC-MS) offers a unique characterization platform that provides both physical and chemical properties of the analyzed polymers. This study presents a library of 11 polymers generated using virgin plastics and post-consumer products. Read More

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Electroblotting through Enzymatic Membranes to Enhance Molecular Tissue Imaging.

J Am Soc Mass Spectrom 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry and Biochemistry, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, United States.

MALDI-TOF mass spectrometry imaging (MSI) is a powerful tool for studying biomolecule localization in tissue. Protein distributions in tissue provide important histological information; however, large proteins exhibit a high limit of detection in MALDI-MS when compared to their corresponding smaller proteolytic peptides. As a result, several techniques have emerged to digest proteins into more detectable peptides for imaging. Read More

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Ovarian clear cell carcinoma with an immature teratoma component showing ARID1A deficiency and an identical PIK3CA mutation.

J Obstet Gynaecol Res 2021 Jun 9. Epub 2021 Jun 9.

Department of Pathology, Jichi Medical University, Tochigi, Japan.

We herein report a case of ovarian clear cell carcinoma with an immature teratoma component that exhibited aggressive behavior. A 47-year-old woman presented with abdominal distention, and computed tomography detected a cystic mass on the right ovary. The resected mass had mural nodules, most of which showed a pale-yellow appearance; some nodules had a heterogeneous cut surface with bright yellow and white areas. Read More

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Use of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Analysis of Serum Peptidome to Classify and Predict Coronavirus Disease 2019 Severity.

Open Forum Infect Dis 2021 Jun 2;8(6):ofab222. Epub 2021 May 2.

Servicios Científico-Técnicos, Universidad de las Islas Baleares, Palma de Mallorca, Spain.

Background: Classification and early detection of severe coronavirus disease 2019 (COVID-19) patients is required to establish an effective treatment. We tested the utility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to classify and predict the severity of COVID-19.

Methods: We used MALDI-TOF MS to analyze the serum peptidome from 72 patients with COVID-19 (training cohort), clinically classified as mild (28), severe (23), and critical (21), and 20 healthy controls. Read More

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Small Intestinal Bacterial Overgrowth in Subclinical Hypothyroidism of Pregnant Women.

Front Endocrinol (Lausanne) 2021 24;12:604070. Epub 2021 May 24.

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Objective: To evaluate the small intestinal bacterial overgrowth (SIBO) of subclinical hypothyroidism of pregnant women, and explore their possible relevance.

Methods: In total, 224 pregnant women with subclinical hypothyroidism during pregnancy (study group) and 196 pregnant women whose thyroid function was normal (control group) were enrolled in this study. Lactulose-based hydrogen and methane breath test was performed to evaluate the growth of intestinal bacteria. Read More

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Persistent or recurrent Barrett's neoplasia after an endoscopic therapy session is associated with DNA content abnormality and can be detected by DNA flow cytometric analysis of paraffin-embedded tissue.

Mod Pathol 2021 Jun 9. Epub 2021 Jun 9.

University of California at San Francisco, Department of Pathology, San Francisco, CA, USA.

Endoscopic therapy is currently the standard of care for the treatment of high-grade dysplasia (HGD) or intramucosal adenocarcinoma (IMC) in patients with Barrett's esophagus (BE). Visible lesions are treated with endoscopic mucosal resection (EMR), which is often coupled with radiofrequency ablation (RFA). However, endoscopic therapy may require multiple sessions (one session every 2-3 months) and does not always assure complete eradication of neoplasia. Read More

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is a newly isolated human commensal bacterium preventing diet-induced obesity and metabolic disorders in mice.

Gut 2021 Jun 8. Epub 2021 Jun 8.

Louvain Drug Research Institute (LDRI), Metabolism and Nutrition Research Group (MNUT), Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Brussels, Belgium

Objective: To investigate the abundance and the prevalence of J115, a novel butyrate-producing bacterium isolated from the human gut both in the general population and in subjects with metabolic syndrome. To study the impact of this bacterium on host metabolism using diet-induced obese and diabetic mice.

Design: We analysed the presence and abundance of the bacterium in 11 984 subjects using four human cohorts (ie, Human Microbiome Project, American Gut Project, Flemish Gut Flora Project and Microbes4U). Read More

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Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer.

J Exp Clin Cancer Res 2021 Jun 9;40(1):191. Epub 2021 Jun 9.

Experimental Pathology and Therapeutics Group, Research Center (CI-IPOP), Portuguese Institute of Oncology, 4200-072, Porto, Portugal.

Background: Muscle invasive bladder cancer (MIBC) remains amongst the deadliest genitourinary malignancies due to treatment failure and extensive molecular heterogeneity, delaying effective targeted therapeutics. Hypoxia and nutrient deprivation, oversialylation and O-glycans shortening are salient features of aggressive tumours, creating cell surface glycoproteome fingerprints with theranostics potential.

Methods: A glycomics guided glycoproteomics workflow was employed to identify potentially targetable biomarkers using invasive bladder cancer cell models. Read More

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The expression of platelet-derived growth factor, epidermal growth factor, and insulin-like growth factor-II in patients with polycystic ovary syndrome and its correlation with pregnancy outcomes.

Ann Palliat Med 2021 May;10(5):5671-5678

Department of Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder with complex pathogenesis. This study aimed to analyze the expression of platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and insulin-like growth factor-II (IGF-II) in patients with PCOS and its correlation with pregnancy outcomes.

Methods: A total of 104 PCOS patients admitted to the Cangzhou Central Hospital from January 2015 to February 2018 were selected as the PCOS group, and 92 healthy pregnant women who received health examinations in the hospital during the same period were selected as the control group. Read More

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Hydrothermal carbonisation of mechanically dewatered digested sewage sludge-Energy and nutrient recovery in centralised biogas plant.

Water Res 2021 May 25;201:117284. Epub 2021 May 25.

Faculty of Engineering and Natural Sciences, Tampere University, P.O.Box 541, 33104 Tampere University, Finland.

This study aimed to assess the role of hydrothermal carbonisation (HTC) in digestate processing in centralised biogas plants receiving dewatered sludge from regional wastewater treatment plants and producing biomethane and fertilisers. Chemically conditioned and mechanically dewatered sludge was used as such (total solids (TS) 25%) or as diluted (15% TS) with reject water in 30 min or 120 min HTC treatments at 210 °C, 230 °C or 250 °C, and the produced slurry was filtered to produce hydrochars and filtrates. The different hydrochars contributed to 20-55% of the original mass, 72-88% of the TS, 74-87% of the energy content, 71-92% of the carbon, above 86% of phosphorous and 38-64% of the nitrogen present in the original digestates. Read More

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