268 results match your criteria masp1 uain

Protective effect and mechanisms of action of Mongolian medicine Sulongga-4 on pyloric ligation-induced gastroduodenal ulcer in rats.

World J Gastroenterol 2021 Apr;27(16):1770-1784

School of Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia Autonomous Region, China.

Background: Sulongga-4 (SL-4) is a herbal formula used in traditional Mongolian medical clinics for the treatment of peptic ulcers and gastroenteritis, even though its pharmacological mechanism has not been well characterized.

Aim: To evaluate the protective effect and identify the mechanisms of action of SL-4 on gastroduodenal ulcer induced by pyloric ligation (PL) in rats.

Methods: PL was performed to induce gastric and duodenal ulcers in rats, which were then treated with oral SL-4 (1. Read More

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MASP1-related 3MC syndrome in a patient from Turkey.

Am J Med Genet A 2021 Mar 25. Epub 2021 Mar 25.

Department of Medical Genetics, University of Health Sciences, Kanuni Training and Research Hospital, Trabzon, Turkey.

3MC syndrome is a rare condition manifesting with typical facial appearance, postnatal growth deficiency, skeletal manifestations, and genitourinary tract anomalies. 3MC is caused by biallelic pathogenic variants in MASP1, COLEC11, or COLEC10. Here, we report an affected subject of Kurdish origin from Turkey presenting with facial dysmorphisms, such as, hypertelorism, blepharophimosis, blepharoptosis, highly arched eyebrows, umbilical hernia, and caudal appendage. Read More

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MASPs at the crossroad between the complement and the coagulation cascades - the case for COVID-19.

Genet Mol Biol 2021 17;44(1 Suppl 1):e20200199. Epub 2021 Mar 17.

Universidade Federal do Paraná (UFPR), Departamento de Genética, Laboratório de Genética Molecular Humana, Curitiba, PR, Brazil.

Components of the complement system and atypical parameters of coagulation were reported in COVID-19 patients, as well as the exacerbation of the inflammation and coagulation activity. Mannose binding lectin (MBL)- associated serine proteases (MASPs) play an important role in viral recognition and subsequent activation of the lectin pathway of the complement system and blood coagulation, connecting both processes. Genetic variants of MASP1 and MASP2 genes are further associated with different levels and functional efficiency of their encoded proteins, modulating susceptibility and severity to diseases. Read More

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Hepatitis B Virus Infection Among Leprosy Patients: A Case for Polymorphisms Compromising Activation of the Lectin Pathway and Complement Receptors.

Front Immunol 2020 11;11:574457. Epub 2021 Feb 11.

Laboratory of Molecular Immunopathology, Postgraduate Program in Internal Medicine and Health Sciences, Department of Clinical Pathology, Hospital de Clínicas, Federal University of Paraná, Curitiba, Brazil.

Thousands of leprosy patients not only suffer from physical deformities, but also either have or have had hepatitis B virus (HBV) coinfection. Polymorphisms of the complement system modulate susceptibility to leprosy, but genetic susceptibility to past or present HBV infection is unknown. We used sequencing and multiplex sequence-specific PCR to genotype 72 polymorphisms of seven genes (, ) encoding components of the lectin pathway, and two genes encoding complement receptors () in 190 patients, of which 74 were positive for HBsAg and/or anti-HBc (HBV+, 93. Read More

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February 2021

Recombinant Silk Proteins with Additional Polyalanine Have Excellent Mechanical Properties.

Int J Mol Sci 2021 Feb 3;22(4). Epub 2021 Feb 3.

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

This paper explores the structures of exogenous protein molecules that can effectively improve the mechanical properties of silkworm silk. Several transgenic vectors fused with the silkworm fibroin light chain and type 3 repeats in different multiples of the ampullate dragline silk protein 1 (MaSp1) from black widow spider with different lengths of the polyalanine motifs were constructed for this study. Transgenic silkworms were successfully obtained by Bac-mediated microinjection. Read More

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February 2021

Evaluation of MASP1, CMPF and U.A serum levels in pre-diabetic subjects in comparison to Normal individuals for early diagnosis of subjects with pre-diabetes, a case-control study.

J Diabetes Metab Disord 2020 Dec 21;19(2):1593-1598. Epub 2020 Nov 21.

Department of Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Background: The present study designed to evaluate the Serum levels of CMPF, MASP1 and UA in pre-diabetic subjects was compared to normal subjects

Methods: This research is a case-control study. We studied 44 pre-diabetics with 44 normal subjects and were evaluated serum concentration of CMPF, Masp1 and U.Ain both groups andfurthermore serum concentration FPG, BUN, Cr, Cho, TG, HDLc, LDLc, AST, ALT, ALP, HbA1c was examined and correlation between of CMPF, Masp1 and U. Read More

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December 2020

The common house spider, Parasteatoda tepidariorum, maintains silk gene expression on sub-optimal diet.

PLoS One 2020 9;15(12):e0237286. Epub 2020 Dec 9.

Department of Biology, Washington and Lee University, Lexington, VA, United States of America.

Cobweb weaving spiders and their relatives spin multiple task-specific fiber types. The unique material properties of each silk type result from differences in amino acid sequence and structure of their component proteins, primarily spidroins (spider fibrous proteins). Amino acid content and gene expression measurements of spider silks suggest some spiders change expression patterns of individual protein components in response to environmental cues. Read More

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January 2021

Unpredictable recombination of PB transposon in Silkworm: a potential risk.

Mol Genet Genomics 2021 Mar 17;296(2):271-277. Epub 2020 Nov 17.

State Key Laboratory of Silkworm Genome Biology, College of Textile and Garment, Southwest University, No. 2 Tiansheng Street, Beibei, Chongqing, 400715, China.

The piggyBac (PB) transposon is the most widely used vector for generating transgenic silkworms. The stability of the PB transposon in the receptor is a serious concern that requires attention because of biosafety concerns. In this study, we found that the transgene silkworm developed loss of reporter gene traits. Read More

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Comprehensive Identification of the Human Secretome as Potential Indicators in Treatment Outcome of HPV-Positive and -Negative Cervical Cancer Patients.

Gynecol Obstet Invest 2020 10;85(5):405-415. Epub 2020 Nov 10.

The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: The aim of this work was to explore the novel and promising biomarkers for the diagnosis and prognosis of cervical cancer patients.

Methods: The secretome of primary cervical tissues was extracted and then determined by the LC-MS/MS assay. The level of screened targets was confirmed using the RT-PCR and ELISA in cervical cancer tissue samples. Read More

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Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role.

Acta Neuropathol Commun 2020 10 28;8(1):174. Epub 2020 Oct 28.

Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via Mario Negri 2, 20156, Milan, Italy.

The lectin pathway (LP) of complement activation is believed to contribute to brain inflammation. The study aims to identify the key components of the LP contributing to TBI outcome as possible novel pharmacological targets. We compared the long-term neurological deficits and neuropathology of wild-type mice (WT) to that of mice carrying gene deletions of key LP components after experimental TBI. Read More

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October 2020

Meta-analysis reveals materiomic relationships in major ampullate silk across the spider phylogeny.

J R Soc Interface 2020 09 30;17(170):20200471. Epub 2020 Sep 30.

Evolution and Ecology Research Centre and School of Biological Earth and Environmental Sciences, UNSW, Sydney, Australia.

Spider major ampullate (MA) silk, with its combination of strength and extensibility, outperforms any synthetic equivalents. There is thus much interest in understanding its underlying materiome. While the expression of the different silk proteins (spidroins) appears an integral component of silk performance, our understanding of the nature of the relationship between the spidroins, their constituent amino acids and MA silk mechanics is ambiguous. Read More

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September 2020

Expanding Canonical Spider Silk Properties through a DNA Combinatorial Approach.

Materials (Basel) 2020 Aug 14;13(16). Epub 2020 Aug 14.

Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA.

The properties of native spider silk vary within and across species due to the presence of different genes containing conserved repetitive core domains encoding a variety of silk proteins. Previous studies seeking to understand the function and material properties of these domains focused primarily on the analysis of dragline silk proteins, MaSp1 and MaSp2. Our work seeks to broaden the mechanical properties of silk-based biomaterials by establishing two libraries containing genes from the repetitive core region of the native silk genome (Library A: genes , , , ; Library B: genes , , , ). Read More

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Nearly complete H, C and N chemical shift assignment of monomeric form of N-terminal domain of Nephila clavipes major ampullate spidroin 2.

Biomol NMR Assign 2020 10 6;14(2):335-338. Epub 2020 Aug 6.

Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Spider dragline silk is well recognized due to its excellent mechanical properties. Dragline silk protein mainly consists of two proteins, namely, major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2). The MaSp N-terminal domain (NTD) conformation displays a strong dependence on ion and pH gradients, which is crucial for the self-assembly behavior of spider silk. Read More

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October 2020

Association of Polymorphisms of , , and Genes with 3MC Syndrome.

Int J Mol Sci 2020 Jul 31;21(15). Epub 2020 Jul 31.

Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.

The Malpuech, Michels, Mingarelli, Carnevale (3MC) syndrome is a rare, autosomal recessive genetic- disorder associated with mutations in the , or genes. The number of 3MC patients with known mutations in these three genes reported so far remains very small. To date, 16 mutations in , 12 mutations in and three in associated with 3MC syndrome have been identified. Read More

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Structural Characterization of Black Widow Spider Dragline Silk Proteins CRP1 and CRP4.

Molecules 2020 Jul 14;25(14). Epub 2020 Jul 14.

Departments of Chemistry and Biological Sciences, University of the Pacific, Stockton, CA 95211, USA.

Spider dragline silk represents a biomaterial with outstanding mechanical properties, possessing high-tensile strength and toughness. In black widows at least eight different proteins have been identified as constituents of dragline silk. These represent major ampullate spidroins MaSp1, MaSp2, MaSp', and several low-molecular weight cysteine-rich protein (CRP) family members, including CRP1, CRP2, and CRP4. Read More

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Alterations in Systemic Inflammatory Response Following a Half-Marathon Race with a Combined Curcumin and Pomegranate Supplement: A Feasibility Study.

J Diet Suppl 2020 Jul 13:1-17. Epub 2020 Jul 13.

Department Kinesiology, Health Promotion, and Recreation, College of Education, Applied Physiology Laboratory, University of North Texas, Denton, TX, USA.

Endurance running training can lead to gradual accumulation of inflammation and soreness ultimately resulting in overuse injuries. Management of soreness and inflammation with pharmaceuticals (i.e. Read More

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Sacral protuberance with cleft lip and palate: Prenatal presentation of 3MC syndrome.

Am J Med Genet A 2020 07 22;182(7):1812-1814. Epub 2020 May 22.

Division of Maternal Fetal Medicine, School of Medicine, The Johns Hopkins University, Baltimore, Maryland, USA.

3MC syndromes are rare heterogeneous autosomal recessive conditions previously designated as Mingarelli, Malpuech, Michels, and Carnevale syndromes, characterized by dysmorphic facial features, facial clefts, growth restriction, and intellectual disability. 3MC is secondary to mutations in the MASP1, MASP3, COLEC11, and COLEC10 genes. The number of patients with 3MC syndrome with known mutations in the COLEC11 or MASP1 is, to date, less than 50. Read More

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iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma.

Allergy 2020 12 3;75(12):3171-3183. Epub 2020 Jul 3.

Department of Biochemistry and Molecular Biology, Faculty of Biology-Biological Research Centre (CIBUS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

Background: Asthma is heterogeneous disease with different phenotypes, endotypes and severities. Definition of these subgroups requires the identification of biomarkers in biological samples, and serum proteomics is a useful and minimally invasive method for this purpose. Therefore, the aim of this study was to detect serum proteins whose abundance is distinctively associated with different asthma phenotypes (allergic vs nonallergic) or severities. Read More

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December 2020

Properties of two spliceoforms of major ampullate spidroin 1 reveal unique functions of N-linker region.

Int J Biol Macromol 2020 Aug 24;157:67-74. Epub 2020 Apr 24.

Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, People's Republic of China. Electronic address:

Spiders produce diverse silk fibers with distinct properties for daily survival. Among these silk fibers, dragline silk spun by major ampullate gland is used for bridgelines and web radii, exhibiting both outstanding tensile strength and extensibility. Although more and more full-length major ampullate spidroin gene sequences have been reported, the research regarding alternative splicing events of spidroins are rare. Read More

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C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Sci Rep 2020 04 14;10(1):6391. Epub 2020 Apr 14.

Institute of Clinical Chemistry, Hannover Medical School, 30625, Hannover, Germany.

Gout is caused by crystallization of uric acid in the form of monosodium urate (MSU) crystals, which induce a sterile inflammatory response that is hardly distinguishable from microbe-induced inflammatory responses. It is unclear, if MSU crystals (like microbes) are recognized by specific pattern recognition receptors. To identify possible soluble pattern recognition molecules for MSU crystals, we purified MSU-binding proteins from human body fluids. Read More

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Activin A Determines Steroid Levels and Composition in the Fetal Testis.

Endocrinology 2020 07;161(7)

Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Victoria, Australia.

Activin A promotes fetal mouse testis development, including driving Sertoli cell proliferation and cord morphogenesis, but its mechanisms of action are undefined. We performed ribonucleic acid sequencing (RNA-seq) on testicular somatic cells from fetal activin A-deficient mice (Inhba KO) and wildtype littermates at embryonic day (E) E13.5 and E15. Read More

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Adding MASP1 to the lectin pathway-Leprosy association puzzle: Hints from gene polymorphisms and protein levels.

PLoS Negl Trop Dis 2020 04 2;14(4):e0007534. Epub 2020 Apr 2.

Laboratory of Molecular Immunopathology, Department of Clinical Pathology, Clinical Hospital, Federal University of Paraná, Curitiba, Brazil.

Background: Deposition of complement factors on Mycobacterium leprae may enhance phagocytosis. Such deposition may occur through the lectin pathway of complement. Three proteins of the lectin pathway are produced from the gene MASP1: Mannan-binding lectin-associated serine protease 1 (MASP-1) and MASP-3 and mannan-binding lectin-associated protein of 44 kDa (MAp44). Read More

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Complement Activation Is Associated With Mortality in Patients With Necrotizing Soft-Tissue Infections-A Prospective Observational Study.

Front Immunol 2020 31;11:17. Epub 2020 Jan 31.

Laboratory of Molecular Medicine, Department of Clinical Immunology, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark.

We assessed whether different complement factors and complement activation products were associated with poor outcome in patients with necrotizing soft-tissue infection (NSTI). We conducted a prospective, observational study in an intensive care unit where treatment of NSTI is centralized at a national level. In 135 NSTI patients and 65 control patients, admission levels of MASP-1, MASP-2, MASP-3, C4, C3, complement activation products C4c, C3bc, and terminal complement complex (TCC) were assessed. Read More

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February 2021

Cellular uptake, intracellular distribution and degradation of Her2-targeting silk nanospheres.

Int J Nanomedicine 2019 26;14:6855-6865. Epub 2019 Aug 26.

Department of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 60-806, Poland.

Background: The development of nanocarrier technology has attracted great interest in the last decade. Biodegradable spheres made of functionalized silk have considerable potential to be used as drug delivery systems for cancer treatment. A targeting ligand displayed at the surface of a carrier, with a specific affinity towards a particular receptor, can further enhance the accumulation and uptake of nanoparticles at the site of a tumor. Read More

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Genes Implicated in Rare Congenital Inner Ear and Cochleovestibular Nerve Malformations.

Ear Hear 2020 Jul/Aug;41(4):983-989

Neurogenomics Division and Center for Rare Childhood Disorders, Translational Genomics Research Institute, Phoenix, Arizona, USA.

Objective: A small subset of children with congenital hearing loss have abnormal cochleovestibular nerves (i.e., absent, aplastic, or deficient cochlear nerves), with largely unknown etiology. Read More

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January 2020

Structural characterization and mechanical properties of chimeric Masp1/Flag minispidroins.

Biochimie 2020 Jan 26;168:251-258. Epub 2019 Nov 26.

College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, China; Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address:

Dragline silk has the highest tensile strength among the seven types of spider silks due to its abundant polyalanine motifs. Whereas the flagelliform spider silk is most extensible as its composed spidroin is rich in GPGGX motifs. Most of the spider silk proteins are composed of an extensive repetitive domain flanked by N- and C-terminal domains. Read More

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January 2020

Complement lectin pathway protein levels reflect disease activity in juvenile idiopathic arthritis: a longitudinal study of the Nordic JIA cohort.

Pediatr Rheumatol Online J 2019 Sep 9;17(1):63. Epub 2019 Sep 9.

Department of Pediatrics, Aarhus University Hospital, Palle Juul-Jensens Blvd. 99, 8200, Aarhus N, Denmark.

Background: To determine the serum levels of the lectin pathway proteins early in the disease course and 17 years after disease onset and to correlate the protein levels to markers of disease activity in participants from a population-based Nordic juvenile idiopathic arthritis (JIA) cohort. Additionally, to assess the predictive value of lectin pathway proteins with respect to remission status.

Methods: A population-based cohort study of consecutive cases of JIA with a disease onset from 1997 to 2000 from defined geographical areas of Finland, Sweden, Norway and Denmark with 17 years of follow-up was performed. Read More

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September 2019

MASP-1 of the complement system alters fibrinolytic behaviour of blood clots.

Mol Immunol 2019 10 17;114:1-9. Epub 2019 Jul 17.

Experimental Haemostasis Group, Department for BioMedical Research, University of Bern, Bern, Switzerland. Electronic address:

Background: The lectin pathway serine protease mannan-binding lectin-associated serine protease 1 (MASP-1) has been demonstrated to be a major link between complement and coagulation, yet little is known about its interactions with the fibrinolytic system. The aim of this work was to assess the effects of MASP-1 on fibrin clot lysis in different experimental settings.

Methods: Rotational thrombelastometry was used to evaluate the effect of MASP-1 on the lysis of clots formed in whole blood under static conditions. Read More

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October 2019

Exome sequencing in multiple sclerosis families identifies 12 candidate genes and nominates biological pathways for the genesis of disease.

PLoS Genet 2019 06 6;15(6):e1008180. Epub 2019 Jun 6.

Red Española de Esclerosis Múltiple REEM, Madrid, Spain.

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system characterized by myelin loss and neuronal dysfunction. Although the majority of patients do not present familial aggregation, Mendelian forms have been described. We performed whole-exome sequencing analysis in 132 patients from 34 multi-incident families, which nominated likely pathogenic variants for MS in 12 genes of the innate immune system that regulate the transcription and activation of inflammatory mediators. Read More

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MASP-1 Increases Endothelial Permeability.

Front Immunol 2019 3;10:991. Epub 2019 May 3.

Research Laboratory, 3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary.

Pathologically increased vascular permeability is an important dysfunction in the pathomechanism of life-threatening conditions, such as sepsis, ischemia/reperfusion, or hereditary angioedema (HAE), diseases accompanied by uncontrolled activation of the complement system. HAE for example is caused by the deficiency of C1-inhibitor (the main regulator of early complement activation), which leads to edematous attacks threatening with circulatory collapse. We have previously reported that endothelial cells become activated during HAE attacks. Read More

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October 2020