37 results match your criteria markets assembly

High-Quality Reference Genome Sequence for the Oomycete Vegetable Pathogen Phytophthora capsici Strain LT1534.

Microbiol Resour Announc 2021 May 27;10(21):e0029521. Epub 2021 May 27.

School of Integrative Plant Science, Cornell University, Geneva, New York, USA.

The oomycete Phytophthora capsici is a destructive pathogen of a wide range of vegetable hosts, especially peppers and cucurbits. A 94.17-Mb genome assembly was constructed using PacBio and Illumina data and annotated with support from transcriptome sequencing (RNA-Seq) reads. Read More

View Article and Full-Text PDF

Perspectives on the Management of Surplus Dairy Calves in the United States and Canada.

Front Vet Sci 2021 13;8:661453. Epub 2021 Apr 13.

Department of Population Medicine, University of Guelph, Guelph, ON, Canada.

The care of surplus dairy calves is a significant issue for the United States and Canadian dairy industries. Surplus dairy calves commonly experience poor welfare as evidenced by high levels of mortality and morbidity, and negative affective states resulting from limited opportunities to express natural behaviors. Many of these challenges are a result of a disaggregated production system, beginning with calf management at the dairy farm of origin and ending at a calf-raising facility, with some calves experiencing long-distance transportation and commingling at auction markets or assembly yards in the interim. Read More

View Article and Full-Text PDF

A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants.

Front Plant Sci 2021 5;12:626625. Epub 2021 Mar 5.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.

Nervonic acid (NA) is a very-long-chain monounsaturated fatty acid that plays crucial roles in brain development and has attracted widespread research interest. The markets encouraged the development of a refined, NA-enriched plant oil as feedstocks for the needed further studies of NA biological functions to the end commercial application. Plant seed oils offer a renewable and environmentally friendly source of NA, but their industrial production is presently hindered by various factors. Read More

View Article and Full-Text PDF

Genome assembly and population genomic analysis provide insights into the evolution of modern sweet corn.

Nat Commun 2021 02 23;12(1):1227. Epub 2021 Feb 23.

Horticultural Sciences Department, University of Florida, Gainesville, FL, USA.

Sweet corn is one of the most important vegetables in the United States and Canada. Here, we present a de novo assembly of a sweet corn inbred line Ia453 with the mutated shrunken2-reference allele (Ia453-sh2). This mutation accumulates more sugar and is present in most commercial hybrids developed for the processing and fresh markets. Read More

View Article and Full-Text PDF
February 2021

Accelerated air-assisted in-syringe extraction and needle spray ionization coupled with miniature mass spectrometry: A streamlined platform for rapid on-site analysis.

Anal Chim Acta 2020 Nov 28;1136:106-114. Epub 2020 Aug 28.

Chinese Academy of Inspection and Quarantine, Beijing 100176, China. Electronic address:

The present study reports on a streamlined analytical platform for rapid, straightforward, and on-site sample analysis using a miniature mass spectrometer with adequate tandem mass spectrometry (MS/MS) capability. An "all-in-one" workflow is developed combining accelerated air-assisted in-syringe extraction, sorbent-packed membrane clean-up, and needle spray ionization with an in-house built syringe assembly. A custom-made metal needle with a sharp, thin, and conical tip of micrometer-scale served as an integral component of the syringe assembly. Read More

View Article and Full-Text PDF
November 2020

Microbiota assembly, structure, and dynamics among Tsimane horticulturalists of the Bolivian Amazon.

Nat Commun 2020 07 29;11(1):3772. Epub 2020 Jul 29.

Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Selective and neutral forces shape human microbiota assembly in early life. The Tsimane are an indigenous Bolivian population with infant care-associated behaviors predicted to increase mother-infant microbial dispersal. Here, we characterize microbial community assembly in 47 infant-mother pairs from six Tsimane villages, using 16S rRNA gene amplicon sequencing of longitudinal stool and tongue swab samples. Read More

View Article and Full-Text PDF

Design of a Tri-Axial Surface Micromachined MEMS Vibrating Gyroscope.

Sensors (Basel) 2020 May 15;20(10). Epub 2020 May 15.

Department of Information, Electronic and Telecomunication Engineer, Sapienza University of Roma, 00184 Rome, Italy.

Gyroscopes are one of the next killer applications for the MEMS (Micro-Electro-Mechanical-Systems) sensors industry. Many mature applications have already been developed and produced in limited volumes for the automotive, consumer, industrial, medical, and military markets. Plenty of high-volume applications, over 100 million per year, have been calling for low-cost gyroscopes. Read More

View Article and Full-Text PDF

'The problem is small enough, the problem is big enough': a qualitative study of health technology assessment and public policy on drug funding decisions for children.

Int J Equity Health 2020 03 30;19(1):45. Epub 2020 Mar 30.

Centre for Health Economics and Policy Analysis, Department of Health Research Methods Evidence and Impact, McMaster University, Hamilton, Canada.

Background: Public policy approaches to funding paediatric medicines in developed public health systems remain understudied. Current approaches to HTA present a variety of conceptual, methodological and practical problems in the context of child health. This study explores the technical and sociopolitical determinants of public funding decisions on paediatric drugs, through the analysis of interviews with stakeholders involved in or impacted by HTA for child health technologies at the provincial and national levels in Canada. Read More

View Article and Full-Text PDF

Genetic and physical mapping of loci for resistance to blackleg disease in canola (Brassica napus L.).

Sci Rep 2020 03 10;10(1):4416. Epub 2020 Mar 10.

NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, 2650, Australia.

Sustainable canola production is essential to meet growing human demands for vegetable oil, biodiesel, and meal for stock feed markets. Blackleg, caused by the fungal pathogen, Leptosphaeria maculans is a devastating disease that can lead to significant yield loss in many canola production regions worldwide. Breakdown of race-specific resistance to L. Read More

View Article and Full-Text PDF

Talkin' About a Resolution: Issues in the Push for Greater Transparency of Medicine Prices.

Pharmacoeconomics 2020 02;38(2):125-134

Independent Economics Consultant, Madrid, Spain.

At the 2019 World Health Assembly, a significant new resolution was agreed by most countries to start publicly sharing information on the real net prices they pay for medicines in their health systems. The resolution also includes provisions for countries to support other transparency activities. However, an additional proposal to require pharmaceutical companies to submit information on their internal sales figures, internal research and development costs, clinical trial costs and marketing costs for each individual medicine as a condition of registration, and for governments to publish this, was not agreed. Read More

View Article and Full-Text PDF
February 2020

Construction of a High-Density Genetic Linkage Map and QTL Mapping for Growth-Related Traits in Takifugu bimaculatus.

Mar Biotechnol (NY) 2020 Feb 3;22(1):130-144. Epub 2020 Jan 3.

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

Takifugu bimaculatus is a euryhaline species, distributed ranging from the southern Yellow Sea to the South China Sea. Their tolerance to a wide range of salinity and temperature, coupled with a desirable firm texture, makes T. bimaculatus a strong candidate for Takifugu aquaculture in subtropics areas. Read More

View Article and Full-Text PDF
February 2020

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability.

Jens Ducrée

Micromachines (Basel) 2019 Dec 17;10(12). Epub 2019 Dec 17.

[email protected] Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Glasnevin, Dublin 9, Ireland.

The majority of commercially oriented microfluidic technologies provide novel point-of-use solutions for laboratory automation with important areas in the context of the life sciences such as health care, biopharma, veterinary medicine and agrifood as well as for monitoring of the environment, infrastructures and industrial processes. Such systems are often composed of a modular setup exhibiting an instrument accommodating rather conventional actuation, detection and control units which interfaces with a fluidically integrated "Lab-on-a-Chip" device handling (bio-)sample(s) and reagents. As the complex network of tiny channels, chambers and surface-functionalised zones can typically not be properly cleaned and regenerated, these microfluidic chips are mostly devised as single-use disposables. Read More

View Article and Full-Text PDF
December 2019

Manipulating nanoscale structure to control functionality in printed organic photovoltaic, transistor and bioelectronic devices.

Nanotechnology 2020 Feb 14;31(9):092002. Epub 2019 Nov 14.

School of Mathematical and Physical Sciences, Faculty of Science, University of Newcastle, Callaghan, NSW, 2308, Australia. Centre for Organic Electronics, University of Newcastle, Callaghan, NSW, 2308, Australia.

Printed electronics is simultaneously one of the most intensely studied emerging research areas in science and technology and one of the fastest growing commercial markets in the world today. For the past decade the potential for organic electronic (OE) materials to revolutionize this printed electronics space has been widely promoted. Such conviction in the potential of these carbon-based semiconducting materials arises from their ability to be dissolved in solution, and thus the exciting possibility of simply printing a range of multifunctional devices onto flexible substrates at high speeds for very low cost using standard roll-to-roll printing techniques. Read More

View Article and Full-Text PDF
February 2020

Large Plasmid Complement Resolved: Complete Genome Sequencing of MF1298, a Candidate Probiotic Strain Associated with Unfavorable Effect.

Microorganisms 2019 Aug 14;7(8). Epub 2019 Aug 14.

Nofima-Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210, N-1431 Ås, Norway.

Considerable attention has been given to the species Lactobacillus plantarum regarding its probiotic potential. strains have shown health benefits in several studies, and even nonstrain-specific claims are allowed in certain markets. strain MF1298 was considered a candidate probiotic, demonstrating in vitro probiotic properties and the ability to survive passage through the human intestinal tract. Read More

View Article and Full-Text PDF

A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production.

Biotechnol Biofuels 2019 15;12:180. Epub 2019 Jul 15.

1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China.

Background: DNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of microbial cell factories. Amino acids and derived biochemicals are important products in industrial biotechnology with wide application and huge markets. Read More

View Article and Full-Text PDF

Design, fabrication, and characterization of a multidimensional prism.

Appl Opt 2019 Feb;58(6):1419-1425

In this research, a novel multidimensional prism with three distinct 45°, 135°, and 225° inclined optical surfaces were realized by using inclined exposure technology and SU-8 polymer. To obtain a smooth surface, the solvent loss percentage and temperature of post-exposure bake (PEB) are two key factors that need to be well controlled during fabrication. The experimental results showed that surface roughness can achieve 42. Read More

View Article and Full-Text PDF
February 2019

From healing wounds to resorbable electronics, levan can fill bioadhesive roles in scores of markets.

Bioinspir Biomim 2018 11 20;14(1):011001. Epub 2018 Nov 20.

Montana Biopolymers Inc., 119 Cathcart Circle, Winnsboro, SC 29180, United States of America.

Levan is a fructose homopolysaccharide which gained attention recently for its unusual combination of properties distinguishing it from other natural biodegradable polysaccharides like chitosan, cellulose or starch. Among the strongest bioadhesives, film-forming levan is garnering interest for its role in some simple solutions to difficult problems. One of these is illustrated by the elegant research using laser-based techniques to construct levan films for healing wounds and burned tissue. Read More

View Article and Full-Text PDF
November 2018

Current strategies are inadequate to curb the rise of tobacco use in Africa.

N Peer

S Afr Med J 2018 Jun 26;108(7):551-556. Epub 2018 Jun 26.

Non-communicable Diseases Research Unit, South African Medical Research Council, Durban, South Africa.

Recently, there have been significant advances in the battle against tobacco use in Africa, with achievements including ratification of the World Health Organization Framework Convention on Tobacco Control (WHO FCTC) and the passing of tobacco control legislation in several countries. Many African countries have achieved measured success, while Uganda, South Africa and Mauritius have accomplished significantly more in their efforts to curb tobacco use. Nevertheless, few African countries meet the standards of the individual WHO FCTC articles with regard to comprehensive implementation. Read More

View Article and Full-Text PDF

[Identification of water buffalo horn and its adulterants using COI barcode].

Yao Xue Xue Bao 2017 Mar;52(3):494-9

Bubali cornu (water buffalo horn) has been used as the substitute for Cornu rhinoceri asiatici (rhino horn) in clinical applications, and is the essential ingredient of Angong Niuhuang Wan. In recent years, there are a number of adulterants on the commercial herbal medicine markets. An efficient tool is required for species identification. Read More

View Article and Full-Text PDF

Immobilization of Cellulase on Magnetic Nanocarriers.

ChemistryOpen 2016 Jun 29;5(3):183-187. Epub 2016 Apr 29.

Technical University of Munich Boltzmannstraße 15 85748 Garching bei München Germany.

The constant increase in the number of sustainable products on the global markets demands new biotechnological processing strategies such as the purification and recovery of biocatalysts. Superparamagnetic iron oxide nanoparticles exhibit excellent recovery properties as carrier materials in enzyme catalysis. We present the simple and fast electrostatic assembly of cellulase (CEL) and low-priced silica-coated magnetic nanoparticles, which demonstrates stable enzyme bonding and excellent colloidal stability. Read More

View Article and Full-Text PDF

Status of New Vaccine Introduction - Worldwide, September 2016.

MMWR Morb Mortal Wkly Rep 2016 Oct 21;65(41):1136-1140. Epub 2016 Oct 21.

Since the global Expanded Program on Immunization (EPI) was launched in 1974, vaccination against six diseases (tuberculosis, polio, diphtheria, tetanus, pertussis, and measles) has prevented millions of deaths and disabilities (1). Significant advances have been made in the development and introduction of vaccines, and licensed vaccines are now available to prevent 25 diseases (2,3). Historically, new vaccines only became available in low-income and middle-income countries decades after being introduced in high-income countries. Read More

View Article and Full-Text PDF
October 2016

De Novo Transcriptome Sequencing Analysis of cDNA Library and Large-Scale Unigene Assembly in Japanese Red Pine (Pinus densiflora).

Int J Mol Sci 2015 Dec 4;16(12):29047-59. Epub 2015 Dec 4.

Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishi Tokyo, Tokyo 188-0002, Japan.

Japanese red pine (Pinus densiflora) is extensively cultivated in Japan, Korea, China, and Russia and is harvested for timber, pulpwood, garden, and paper markets. However, genetic information and molecular markers were very scarce for this species. In this study, over 51 million sequencing clean reads from P. Read More

View Article and Full-Text PDF
December 2015

A microbial platform for renewable propane synthesis based on a fermentative butanol pathway.

Biotechnol Biofuels 2015 10;8:61. Epub 2015 Apr 10.

BBSRC/EPSRC Centre for Synthetic Biology of Fine and Speciality Chemicals, Manchester Institute of Biotechnology, Faculty of Life Sciences, 131 Princess Street, The University of Manchester, Manchester, M1 7DN UK.

Background: Propane (C3H8) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel, in addition to existing global markets and infrastructure for storage, distribution and utilization in a wide range of applications. Consequently, propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. This study focuses on the construction and evaluation of alternative microbial biosynthetic pathways for the production of renewable propane. Read More

View Article and Full-Text PDF

Trigger chemistries for better industrial formulations.

ACS Appl Mater Interfaces 2015 Apr 23;7(12):6369-82. Epub 2015 Mar 23.

⊥Formulation Science, Corporate Research and Development, The Dow Chemical Company, Collegeville, Pennsylvania 19426, United States.

In recent years, innovations and consumer demands have led to increasingly complex liquid formulations. These growing complexities have provided industrial players and their customers access to new markets through product differentiation, improved performance, and compatibility/stability with other products. One strategy for enabling more complex formulations is the use of active encapsulation. Read More

View Article and Full-Text PDF

New frontiers for encapsulation in the chemical industry.

ACS Appl Mater Interfaces 2015 Apr 23;7(12):6359-68. Epub 2015 Mar 23.

∥Formulation Science, Corporate Research and Development, The Dow Chemical Company, Collegeville, Pennsylvania 19426, United States.

Encapsulation of actives comprises an area of exploration undergoing rapid growth in both academic and industrial research settings. Encapsulation processes are employed as a part of product synthesis processes for improved efficiency, enhanced stability, active ingredient compatibility, increased safety, targeted delivery, and novel performance of the end product. Such technical benefits enable producers to offer products with increased formulation complexity, access new markets, differentiate products, and improve compatibility and stability, while meeting consumer demands with improved performance, reduced costs, and new actives. Read More

View Article and Full-Text PDF

Metabolism of acyl-lipids in Chlamydomonas reinhardtii.

Plant J 2015 May 3;82(3):504-522. Epub 2015 Mar 3.

School of Biological Sciences and Center for Biological Chemistry, University of Nebraska - Lincoln, Lincoln, NE, 68588, USA.

Microalgae are emerging platforms for production of a suite of compounds targeting several markets, including food, nutraceuticals, green chemicals, and biofuels. Many of these products, such as biodiesel or polyunsaturated fatty acids (PUFAs), derive from lipid metabolism. A general picture of lipid metabolism in microalgae has been deduced from well characterized pathways of fungi and land plants, but recent advances in molecular and genetic analyses of microalgae have uncovered unique features, pointing out the necessity to study lipid metabolism in microalgae themselves. Read More

View Article and Full-Text PDF

Hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins.

J Pharm Pharmacol 2015 Mar 22;67(3):338-50. Epub 2014 Sep 22.

Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, UK.

Objectives: Glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. This review will focus on the latest studies attempting to exploit bacterial N-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. The challenges that remain for these approaches to reach full biotechnological maturity will be discussed. Read More

View Article and Full-Text PDF

Mail-order microfluidics: evaluation of stereolithography for the production of microfluidic devices.

Lab Chip 2014 Apr;14(7):1294-301

Department of Bioengineering, University of Washington, Seattle, WA, USA.

The vast majority of microfluidic devices are developed in PDMS by molding ("soft lithography") because PDMS is an inexpensive material, has physicochemical properties that are well suited for biomedical and physical sciences applications, and design cycle lengths are generally adequate for prototype development. However, PDMS molding is tediously slow and thus cannot provide the high- or medium-volume production required for the commercialization of devices. While high-throughput plastic molding techniques (e. Read More

View Article and Full-Text PDF

De novo assembly and functional annotation of the olive (Olea europaea) transcriptome.

DNA Res 2013 Feb 7;20(1):93-108. Epub 2013 Jan 7.

Department of Integrated Bioinformatics, National Institute for Bioinformatics, University of Málaga, Campus de Teatinos, Málaga, Spain.

Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. Read More

View Article and Full-Text PDF
February 2013

Passive millimeter wave imaging sensors for commercial markets.

Appl Opt 2010 Jul;49(19):E7-12

Microelectronics Laboratory, HRL Laboratories, LLC, 3011 Malibu Canyon Road, Malibu, California 90265, USA.

We describe the development of passive millimeter wave imaging sensors, operating at W band, that are currently being manufactured for commercial markets using standard automated assembly processes. A description of HRL Laboratories' millimeter wave imaging chipset is presented, focusing on parameters that limit sensor performance, such as detector 1/f noise, low noise amplifier noise figure, and gain drift. We conclude with a discussion of ongoing research and development in passive millimeter wave imaging and performance improvements that can be expected for future imaging sensors. Read More

View Article and Full-Text PDF