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Direct Reprogramming Induces Vascular Regeneration Post Muscle Ischemic Injury.

Mol Ther 2021 Jul 28. Epub 2021 Jul 28.

Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA, 10029; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA, 10029; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA, 10029. Electronic address:

Reprogramming non-cardiomyocytes (non-CMs) into cardiomyocyte (CM)-like cells is a promising strategy for cardiac regeneration in conditions such as ischemic heart disease. Here, we used a modified mRNA (modRNA) gene delivery platform to deliver a cocktail of four cardiac-reprogramming genes (Gata4 (G), Mef2c (M), Tbx5 (T) and Hand2 (H)) together with three reprogramming-helper genes (Dominant Negative (DN)-TGFβ, DN-Wnt8a and Acid ceramidase (AC)), termed 7G-modRNA, to induce CM-like cells. We showed that 7G-modRNA reprogrammed 57% of CM-like cells in vitro. Read More

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Defining the Role of Cellular Immune Signatures in Diagnostic Evaluation of Suspected Tuberculosis.

J Infect Dis 2021 Jul 31. Epub 2021 Jul 31.

TB Research Centre, National Heart and Lung Institute, Imperial College London, London, United Kingdom.

Background: Diagnosis of paucibacillary tuberculosis (TB) including extrapulmonary TB is a significant challenge, particularly in high-income, low-incidence settings. Measurement of Mycobacterium tuberculosis (Mtb)-specific cellular immune signatures by flow cytometry discriminates active TB from latent TB infection (LTBI) in case-control studies; however, their diagnostic accuracy and clinical utility in routine clinical practice is unknown.

Methods: Using a nested case-control study design within a prospective multicenter cohort of patients presenting with suspected TB in England, we assessed diagnostic accuracy of signatures in 134 patients who tested interferon-gamma release assay (IGRA)-positive and had final diagnoses of TB or non-TB diseases with coincident LTBI. Read More

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Sauna dehydration as a new physiological challenge model for intestinal barrier function.

Sci Rep 2021 Jul 30;11(1):15514. Epub 2021 Jul 30.

Nutrition-Gut-Brain Interactions Research Centre, Faculty of Medicine and Health, School of Medical Sciences, Örebro University, 701 82, Örebro, Sweden.

The intestinal barrier plays a crucial role in maintaining gut health, and an increased permeability has been linked to several intestinal and extra-intestinal disorders. There is an increasing demand for interventions aimed at strengthening this barrier and for in vivo challenge models to assess their efficiency. This study investigated the effect of sauna-induced dehydration on intestinal barrier function (clinicaltrials. Read More

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Novel method of real-time PCR-based screening for common fetal trisomies.

BMC Med Genomics 2021 Jul 30;14(1):195. Epub 2021 Jul 30.

Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Korea.

Background: The non-invasive prenatal test (NIPT) is based on next generation sequencing (NGS) and is used for screening for fetal trisomy. However, it is time-consuming and technically difficult. Recently, peptide nucleic acid (PNA) probe-based real-time polymerase chain reaction (RT-PCR) was developed. Read More

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Immune-Related lncRNA Signature for Predicting the Immune Landscape of Head and Neck Squamous Cell Carcinoma.

Front Mol Biosci 2021 13;8:689224. Epub 2021 Jul 13.

Department of Otorhinolaryngology, Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China.

Long non-coding RNA (lncRNA) plays a significant role in the development, establishment, and progression of head and neck squamous cell carcinoma (HNSCC). This article aims to develop an immune-related lncRNA (irlncRNA) model, regardless of expression levels, for risk assessment and prognosis prediction in HNSCC patients. We obtained clinical data and corresponding full transcriptome expression of HNSCC patients from TCGA, downloaded GTF files to distinguish lncRNAs from Ensembl, discerned irlncRNAs based on co-expression analysis, distinguished differentially expressed irlncRNAs (DEirlncRNAs), and paired these DEirlncRNAs. Read More

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Isolated optic neuropathy due to folate deficiency with associated iron overload.

BMJ Case Rep 2021 Jul 29;14(7). Epub 2021 Jul 29.

Clinical Sciences, Florida State University College of Medicine, Tallahassee, Florida, USA

Isolated optic neuropathy due to folate deficiency is rarely reported. Poor dietary practices, malabsorption, and tobacco/alcohol abuse are usually responsible. We examined a patient with blinding optic neuropathies and isolated folic acid deficiency. Read More

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Predictive protein markers for depression severity in mood disorders: A preliminary trans-diagnostic approach study.

J Psychiatr Res 2021 Jul 24;142:63-72. Epub 2021 Jul 24.

Department of Neuropsychiatry, Seoul National University Hospital, Seoul, Republic of Korea; Department of Psychiatry and Behavioral Science, Seoul National University College of Medicine, Seoul, Republic of Korea; Institute of Human Behavioral Medicine, Seoul National University Medical Research Center, Seoul, Republic of Korea. Electronic address:

Depression is a common symptom of many mental disorders, especially major depressive disorder (MDD) and bipolar disorder (BD). Previous studies have reported that these diseases share common pathophysiological pathways; therefore, this study elucidated whether the plasma levels of protein markers related to common depressive symptoms differed between patients with BD and those with MDD. Plasma samples of 71 patients with mood disorders and clinical manifestations were analyzed in this study. Read More

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Cross-diagnostic evaluation of minor physical anomalies in psychiatric disorders.

J Psychiatr Res 2021 Jul 20;142:54-62. Epub 2021 Jul 20.

Department of Psychiatry, National Institute for Mental Health and Neurosciences (NIMHANS), Bangalore, India. Electronic address:

Background: Minor physical anomalies (MPA) are markers of impaired neurodevelopment during the prenatal stage. Assessing MPA across psychiatric disorders may help understand their shared nature. In addition, MPA in family members would indicate a shared liability and endophenotype potential. Read More

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Prolonged anesthesia and decreased toxicity of enantiomeric-excess Bupivacaine loaded in Ionic gradient liposomes.

Int J Pharm 2021 Jul 26:120944. Epub 2021 Jul 26.

Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (Unicamp), Campinas, Brazil. Electronic address:

Bupivacaine is the most employed local anesthetic in surgical procedures, worldwide. Its systemic toxicity has directed the synthesis of the less toxic, S(-) enantiomer. This work describes a formulation of ionic gradient liposomes (IGL) containing BVC, an enantiomeric excess mixture of 75% S(-) and 25% R(+) bupivacaine. Read More

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The role of procalcitonin and presepsin in the septic febrile neutropenia in acute leukemia patients.

PLoS One 2021 29;16(7):e0253842. Epub 2021 Jul 29.

Department of Internal Medicine (Hematology), Faculty of Medicine, Damascus University, Damascus, Syria.

Background: The source of bacterial infection in neutropenic acute leukemia patients is detected in about 20-30% of cases. Bacterial cultures may require a long incubation period and risk false-positive and false- negative results. Therefore, biomarkers distinguishing septic febrile neutropenia from other etiologies in acute leukemia patients play the important role in patient assessment and treatment planning. Read More

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Wnt Family Member 9b (Wnt9b) Is a New Sensitive and Specific Marker for Breast Cancer.

Am J Surg Pathol 2021 Jul 29. Epub 2021 Jul 29.

Department of Pathology and Laboratory Medicine, Warren Alpert Medical School of Brown University, Providence, RI.

Confirming the tumor origin is often a diagnostic challenge in pathology and carries significant therapeutic impacts. Cytokeratin 7, estrogen receptor, and GATA binding protein 3 (GATA3) are well-established diagnostic markers frequently used to support a tumor's breast origin. However, their specificities still have room to improve. Read More

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Genome Instability-Derived Genes Are Novel Prognostic Biomarkers for Triple-Negative Breast Cancer.

Front Cell Dev Biol 2021 12;9:701073. Epub 2021 Jul 12.

Cancer Centre and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau, China.

Background: Triple-negative breast cancer (TNBC) is an aggressive disease. Recent studies have identified genome instability-derived genes for patient outcomes. However, most of the studies mainly focused on only one or a few genome instability-related genes. Read More

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Cecal Ligation and Puncture-Induced Sepsis Promotes Brown Adipose Tissue Inflammation Without Any Impact on Expression of Thermogenic-Related Genes.

Front Physiol 2021 12;12:692618. Epub 2021 Jul 12.

Department of Clinical Pharmacy and Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.

The negative effects of chronic low-level inflammation on adipose tissue physiology have been extensively demonstrated, whereas the effects of acute inflammation are less studied. Here, we aimed to investigate the effects of sepsis-induced acute inflammation on gene expression markers of brown and white adipose tissue functionality. Brown adipose tissue (BAT) and perirenal white adipose tissue (prWAT) gene expression markers were analyzed in cecal ligation and puncture (CLP)-induced sepsis mice model. Read More

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Genetic diversity and population structure of ridge gourd (Luffa acutangula) accessions in a Thailand collection using SNP markers.

Sci Rep 2021 Jul 28;11(1):15311. Epub 2021 Jul 28.

Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand.

This study explored a germplasm collection consisting of 112 Luffa acutangula (ridge gourd) accessions, mainly from Thailand. A total of 2834 SNPs were used to establish population structure and underlying genetic diversity while exploring the fruit characteristics together with genetic information which would help in the selection of parental lines for a breeding program. The study found that the average polymorphism information content value of 0. Read More

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Biomarkers for Impending Risk of Osteoporosis in Premenopausal Women.

J Coll Physicians Surg Pak 2021 Aug;31(8):910-915

Department of Biological & Biomedical Sciences, The Aga Khan University, Karachi, Pakistan.

Objective: To compare 'cytokines' and 'bone turnover markers' in pre- and post-menopausal women and identify their relationship with bone mineral density (BMD) in both groups.  Study Design: A cross-sectional study.

Place And Duration Of Study: Department of Biological & Biomedical Sciences, The Aga Khan University, Karachi, Pakistan, from June 2017 to August 2019. Read More

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Desmoplastic Small Round Cell Tumor of the Submandibular Gland: A Case Report and Literature Review.

ORL J Otorhinolaryngol Relat Spec 2021 Jul 28:1-6. Epub 2021 Jul 28.

Department of Pathology, Maoming People's Hospital, Maoming, China.

Desmoplastic small round cell tumor (DSRCT) is a rare and aggressively malignant tumor mostly occurring in the abdominal and pelvic cavity of young patients. However, few cases had been reported concerning DSRCT occurring in the head and neck region. We presented a rare case of DSRCT of the right submandibular in a 25-year-old man. Read More

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Different patterns in root and soil fungal diversity drive plant productivity of the desert truffle Terfezia claveryi in plantation.

Environ Microbiol 2021 Jul 28. Epub 2021 Jul 28.

Departamento Biología Vegetal, Facultad de Biología, CEIR Campus Mare Nostrum (CMN, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.

The desert truffle Terfezia claveryi is one of the few mycorrhizal fungi currently in cultivation in semiarid and arid areas. Agroclimatic parameters seem to affect its annual yield, but there is no information on the influence of biotic factors. In this study, fungal diversity was analyzed by high-throughput sequencing of the ITS2 rDNA region from soil and root samples to compare productive and non-productive mycorrhizal plants in a 4-years-old plantation (Murcia, Spain). Read More

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Activation and gut-homing of peripheral T cells in HIV immunologic non-responders despite long term viral suppression.

PLoS One 2021 28;16(7):e0254149. Epub 2021 Jul 28.

Department of Medicine, University of Toronto, Toronto, Canada.

Objective: Serious non-AIDS disease events (SNAE) are experienced disproportionately by immunologic non-responders (INRs), HIV-infected individuals who do not restore CD4 T cells in blood despite effective viral suppression. We aimed to characterize the inflammatory biomarker profile of the INR phenotype.

Methods: Blinded cross-sectional cohort study comparing markers of immune activation and gut homing between INR and non-INR individuals. Read More

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Original Article: Clinical Research.

Sarcoidosis Vasc Diffuse Lung Dis 2021 28;38(2):e2021020. Epub 2021 Jun 28.

Department of Internal Medicine, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.

Aim: In this study, we aimed to investigate the possible role of endotrophin, a profibrotic byproduct of collagen VI, in the complex process of fibrosis development in the disease group with pulmonary fibrosis among interstitial lung diseases.

Material And Method: When the patients' participation in the study were completed, smoking or alcohol drinking conditions, and family history were recorded. Their weights and heights were recorded and body mass index (BMI) was calculated. Read More

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Dihydrotanshinone-I modulates Epithelial Mesenchymal Transition (EMT) Thereby Impairing Migration and Clonogenicity of Triple Negative Breast Cancer Cells.

Asian Pac J Cancer Prev 2021 Jul 1;22(7):2177-2184. Epub 2021 Jul 1.

Department of Medical Oncology, Dr. BRA IRCH, AIIMS, New Delhi, India.

Background: Salvia miltiorrhiza Bunge (Danshen), has been used for its therapeutic value in Traditional Chinese Medicine (TCM), for almost a thousand years. Dihydrotanshinone-I (DHTS) is a lipophilic compound isolated from the plant Salvia miltiorrhiza that has been shown to induce anti-proliferative and apoptotic effects on breast cancer cells. In the present study, we investigated the anti-migratory effect of DHTS on TNBC cell lines by studying the Epithelial Mesenchymal Transition (EMT) changes. Read More

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The Association of the BRAF-V600E Mutation with the Expression of the Molecular Markers in the Primary Tumor and Metastatic Tissue in Papillary Thyroid Cancer.

Asian Pac J Cancer Prev 2021 Jul 1;22(7):2017-2024. Epub 2021 Jul 1.

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation.

Objective: The aim of this study was investigation the AKT / mTOR signaling pathway components, transcriptional and growth factors, as well as steroid hormone receptors and nuclear factors Brn-3α and TRIM16 expression in the tissue of the primary thyroid tumor and metastases, depending on the BRAF- V600E status.

Material And Methods: The study was enrolled 20 patients with PTCs, who underwent surgical treatment. They were divided into negative BRAF-V600E status (12 people), positive BRAF-V600E status (8 patients). Read More

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Assessment and Establishment of Correlation between Reactive Oxidation Species, Citric Acid, and Fructose Level in Infertile Male Individuals: A Machine-Learning Approach.

J Hum Reprod Sci 2021 Apr-Jun;14(2):129-136. Epub 2021 Jun 28.

Department of Studies in Zoology, University of Mysore, Mysore, Karnataka, India.

Background: Biochemical complexity of seminal plasma and obesity has an important role in male infertility (MI); so far, it has not been possible to provide evidence of clinical significance for all of them.

Aims: Our goal here is to evaluate the correlation between biochemical markers with semen parameters, which might play a role in MI.

Study Setting And Design: We enlisted 100 infertile men as patients and 50 fertile men as controls to evaluate the sperm parameters and biochemical markers in ascertaining MI. Read More

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A panel of miRNAs as prognostic markers for African-American patients with triple negative breast cancer.

BMC Cancer 2021 Jul 27;21(1):861. Epub 2021 Jul 27.

Department of Microbiology, Howard University Cancer Center, Howard University, Washington DC, USA.

Background: To investigate the global expression profile of miRNAs, their impact on cellular signaling pathways, and their association with poor prognostic parameters in African-American (AA) patients with triple negative breast cancer (TNBC).

Methods: Twenty-five samples of AA TNBC patients were profiled for global miRNA expression and stratified considering three clinical-pathological parameters: tumor size, lymph node (LN), and recurrence (REC) status. Differential miRNA expression analysis was performed for each parameter, and their discriminatory power was determined by Receiver Operating Characteristic (ROC) curve analysis. Read More

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A simple risk score for detecting radiological occult metastasis in patients with resectable or borderline resectable pancreatic ductal adenocarcinoma.

J Hepatobiliary Pancreat Sci 2021 Jul 27. Epub 2021 Jul 27.

Department of Surgery, Kansai Medical University, Osaka, Japan.

Background: We advocated carbohydrate antigen (CA) 19-9 ≥ 150 U/mL and tumor size ≥ 30 mm as "high-risk markers" for predicting unresectability among patients with radiologically resectable (R) or borderline resectable (BR) pancreatic ductal adenocarcinoma (PDAC). The main aim is to establish a risk scoring system for occult abdominal metastasis (OAM) in R/BR PDAC.

Methods: Predictors of OAM were investigated retrospectively in an experiment cohort from 2006 to 2018. Read More

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Expression of Friend Leukemia Integration-1 (Fli-1) and the Apoptosis Regulator B Cell Lymphoma-2 (BCL-2) in Gastric Carcinoma; an Immunohistochemical Study.

J Immunoassay Immunochem 2021 Jul 27. Epub 2021 Jul 27.

Pathology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, Egypt.

Fli-1 regulates multiple biological functions in different cancers particularly Ewing sarcoma and leukemias. There are controversial reports regarding function and prognostic significance of Fli-1 in epithelial cancer including gastric carcinoma (GC). No previous reports examined relationship between Fli-1 and BCL-2 in GC. Read More

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Contrary to Expectations: Does Context Influence the Processing Cost Associated with Negation?

J Psycholinguist Res 2021 Jul 27. Epub 2021 Jul 27.

Department of Psychology, University of Tübingen, Schleichstraße 4, 72076, Tübingen, Germany.

Negative sentences are hard to process when they are presented out of context. When embedded in a context of plausible denial their processing difficulty decreases or is completely eliminated. We investigated in six behavioral experiments whether the processing of negation is eased in a denial context triggered by discourse markers (e. Read More

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A Unique Epithelioid Vascular Neoplasm of Bone Characterized by EWSR1/FUS-NFATC1/2 Fusions.

Genes Chromosomes Cancer 2021 Jul 26. Epub 2021 Jul 26.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.

An increasing number of epithelioid vascular lesions, in particular tumors from the benign and low-grade end of the spectrum, have been characterized by recurrent gene fusions. As a result, the detection of these molecular markers have improved the classification of diagnostically challenging cases. However, despite the significant progress, there are occasional lesions that do not fit in known histologic or molecular groups. Read More

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Reactive Intralymphovascular Immunoblastic Proliferations Mimicking Aggressive Lymphomas.

Am J Surg Pathol 2021 Jul 26. Epub 2021 Jul 26.

Department of Hematopathology, The University of Texas MD Anderson Cancer Center Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center, Houston, TX Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA Department of Pathology, Advent Health-Orlando, Orlando, FL Division of Hematopathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.

Reactive intralymphovascular immunoblastic proliferations (ILVIPs) may mimic aggressive lymphomas and are rarely reported. Herein, we characterize the clinicopathologic features of 8 patients with ILVIPs. No patients had lymphadenopathy, hepatosplenomegaly, or other findings suggestive of lymphoma. Read More

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Detection of Alzheimer's disease amyloid beta 1-42, p-tau, and t-tau assays.

Alzheimers Dement 2021 Jul 26. Epub 2021 Jul 26.

Department of Neurology, Mayo Clinic, Rochester, Minnesota, USA.

Introduction: We aimed to provide cut points for the automated Elecsys Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers.

Methods: Cut points for Elecsys amyloid beta 42 (Aβ42), total tau (t-tau), hyperphosphorylated tau (p-tau), and t-tau/Aβ42 and p-tau/Aβ42 ratios were evaluated in Mayo Clinic Study of Aging (n = 804) and Mayo Clinic Alzheimer's Disease Research Center (n = 70) participants.

Results: The t-tau/Aβ42 and p-tau/Aβ42 ratios had a higher percent agreement with normal/abnormal amyloid positron emission tomography (PET) than the individual CSF markers. Read More

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LPS-induced autophagy in human dental pulp cells is associated with p38.

J Mol Histol 2021 Jul 26. Epub 2021 Jul 26.

Department of Operative Dentistry and Endodontics, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 56 Ling yuan Road West, Guangzhou, 510055, China.

Lipopolysaccharides (LPS), which are components of the cell wall of Gram-negative bacteria, are among the important factors that induce inflammation, including pulpitis. Autophagy in human dental pulp cells (hDPCs) acts as a protective mechanism that promotes cell survival under adverse conditions through different signaling pathways. In this study, we examined whether LPS increases autophagy in hDPCs and investigated the role of mitogen-activated protein kinases signaling and nuclear factor κB (NF-κB) in this process. Read More

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