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Doxorubicin suppresses chondrocyte differentiation by stimulating ROS production.

Eur J Pharm Sci 2021 Sep 18:106013. Epub 2021 Sep 18.

Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang City, Guizhou Province, 550004, P. R. China.

Background: Doxorubicin (DOX) is widely used as an effective chemotherapy agent in human cancer. Our study aimed to explore the specific mechanism of DOX in osteoarthritis (OA).

Methods: A mouse OA model was established by destabilizing the medial meniscus (DMM), and the role of DOX was determined by intraperitoneally injecting 5 or 10 mg/kg DOX. Read More

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September 2021

Multiplexed DNA-Directed Patterning of Antibodies for Applications in Cell Subpopulation Analysis.

ACS Appl Mater Interfaces 2021 Sep 21. Epub 2021 Sep 21.

California Institute of Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, California 94720, United States.

Antibodies provide the functional biospecificity that has enabled the development of sensors, diagnostic tools, and assays in both laboratory and clinical settings. However, as multimarker screening becomes increasingly necessary due to the heterogeneity and complexity of human pathology, new methods must be developed that are capable of coordinating the precise assembly of multiple, distinct antibodies. To address this technological challenge, we engineered a bottom-up, high-throughput method in which DNA patterns, comprising unique 20-base pair oligonucleotides, are patterned onto a substrate using photolithography. Read More

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September 2021

[Flow cytometry evaluation of the rhesus monkey cellular immunity following the Zaire ebolavirus (Filoviridae; Ebolavirus: Zaire ebolavirus) experimental infection].

Vopr Virusol 2021 Sep 18;66(4):289-298. Epub 2021 Sep 18.

Directorate of the Chief of the Radiation, Chemical, and Biological Defence Troops of the Armed Forces of the Ministry of Defence of the Russian Federation.

Introduction: The outbreaks of the Zaire ebolavirus (ZE) disease (ZED) that have arisen in the last decade determine the need to study the infection pathogenesis, the formation of specific immunity forming as well as the development of effective preventive and therapeutic means. All stages of fight against the ZED spread require the experimental infection in sensitive laboratory animals, which are rhesus monkeys in case of this disease .The aim of the study is to evaluate the rhesus monkey cellular immunity following the ZE experimental infection by the means of flow cytometry (cytofluorimetry). Read More

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September 2021

Evaluation of exposure to environmental stressors on heat-shock protein 70 expression in normal oral keratinocyte cells.

Clin Exp Dent Res 2021 Sep 20. Epub 2021 Sep 20.

School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Objectives: This study aimed to investigate the effect of cortisol, estrogen, and nicotine on heat shock protein 70 (HSP70) expressions at the level of normal oral mucosa keratinocyte cells.

Methods: In this in vitro study, keratinocytes were derived from rat oral cavity and cultured. Stressors were applied, including three groups, group 1: estrogen to simulate the postmenopausal state; group 2: cortisol to simulate psychological stress situation; group 3: nicotine to simulate smoking state. Read More

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September 2021

Scrotal wall leiomyosarcoma: a case report and review of the literature.

J Med Case Rep 2021 Sep 21;15(1):465. Epub 2021 Sep 21.

Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Background: Up to 30% of all scrotal masses are sarcomas. Leiomyosarcoma of the scrotal wall is rare, and its clinical significance and prognosis have not been well defined, since the most reported cases have little or no follow-up.

Case Presentation: We report a 45-year-old Caucasian man who was admitted with a firm, nontender, mobile scrotal wall mass from 15 months ago. Read More

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September 2021

Imaging mass cytometry for high-dimensional tissue profiling in the eye.

BMC Ophthalmol 2021 Sep 20;21(1):338. Epub 2021 Sep 20.

Faculty of Medicine, Eye Center, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany.

Background: Imaging mass cytometry (IMC) combines the principles of flow cytometry and mass spectrometry (MS) with laser scanning spatial resolution and offers unique advantages for the analysis of tissue samples in unprecedented detail. In contrast to conventional immunohistochemistry, which is limited in its application by the number of possible fluorochrome combinations, IMC uses isoptope-coupled antibodies that allow multiplex analysis of up to 40 markers in the same tissue section simultaneously.

Methods: In this report we use IMC to analyze formalin-fixed, paraffin-embedded conjunctival tissue. Read More

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September 2021

Increased LPS levels coexist with systemic inflammation and result in monocyte activation in severe COVID-19 patients.

Int Immunopharmacol 2021 Sep 9;100:108125. Epub 2021 Sep 9.

Laboratory of Cellular and Molecular Immunology, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil; Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil; Graduate Program in Biosciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil. Electronic address:

Mucosal barrier alterations may play a role in the pathogenesis of several diseases, including COVID-19. In this study we evaluate the association between bacterial translocation markers and systemic inflammation at the earliest time-point after hospitalization and at the last 72 h of hospitalization in survivors and non-survivors COVID-19 patients. Sixty-six SARS-CoV-2 RT-PCR positive patients and nine non-COVID-19 pneumonia controls were admitted in this study. Read More

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September 2021

Critical Evaluation of Different Lysosomal Labeling Methods Used to Analyze RNA Nanocarrier Trafficking in Cells.

Bioconjug Chem 2021 Sep 20. Epub 2021 Sep 20.

Department of Biomedical Engineering, University of Delaware, 161 Colburn Lab, Newark, Delaware 19716, United States.

The use of nucleic acids to regulate gene expression is a rapidly developing field with immense clinical potential. Nanomaterials are frequently used to deliver nucleic acids into cells as they can overcome the poor cellular uptake and endo/lysosomal degradation of bare nucleic acids. For these nanocarriers to be effective, they must escape endo/lysosomal compartments to deliver their nucleic acid cargo into the cytosol (for ribonucleic acid (RNA)) or nucleus (for deoxyribonucleic acid (DNA)). Read More

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September 2021

Endometriosis is associated with an increased whole-blood thrombogenicity detected by a novel automated microchip flow-chamber system (T-TAS®).

Ginekol Pol 2021 Sep 20. Epub 2021 Sep 20.

Division of Reproduction, Poznan University of Medical Sciences, Poland.

Objectives: Potential thrombotic and antifibrinolytic influence of endometriosis on haemostasis has been recently reported in the literature, as well as increased cardiovascular morbidity in women suffering from the disease. We performed a pilot study to assess the influence of endometriosis on the thrombus formation process underin vitroflow conditions.

Material And Methods: This study compared women with confirmed endometriosis (n = 23) surgically and control healthy subjects (n = 10). Read More

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September 2021

Isolation and Separation of Epithelial CD34 Cancer Stem Cells from -deficient Squamous Cell Carcinoma.

Bio Protoc 2017 Sep 5;7(17):e2524. Epub 2017 Sep 5.

Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation strategies must preserve their viability. The ability to isolate different populations of cells from the bulk of the tumor will continue to deepen our understanding of the biology of cancer stem cells. Read More

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September 2017

One-step White Blood Cell Extracellular Staining Method for Flow Cytometry.

Bio Protoc 2021 Aug 20;11(16):e4135. Epub 2021 Aug 20.

Department of Research and Development, Beckman Coulter Life Sciences-Immunotech, Marseille, France.

Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell lysis are carried out in a single step, using a gentle lysis solution mixed with fluorescent antibody conjugates or probes in a dry or liquid format. The blood sample may be obtained from a routine venipuncture or directly from a fingerprick, allowing for near-patient analysis. Read More

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Analysing "tear-drop" prints of acute promyelocytic leukemia (APML): immunophenotypic prognostication of APML by FCM.

Am J Blood Res 2021 15;11(4):446-457. Epub 2021 Aug 15.

ICMR-National Institute of Pathology, Safdarjung Hospital Campus Ansari Nagar, New Delhi, India.

Introduction: Acute promyelocytic leukemia (APML), although genetically and morphologically distinct from other AML (acute myeloid leukemia) subtypes, is one of the most best responsive acute myeloid leukemia. -Conventional diagnostic methods and morphological hints often fail in the majority of the cases in the peripheral laboratories owing to resource constraints, unavailability of cytogenetic work-up, hypogranular variants, morphological mimicry by AML-monocytic and myelo-monocytic, etc. Flowcytometry (FCM), however, can be utilized as a feasible and reliable immunophenotypic diagnostic and prognostic tool for prompt identification of APML. Read More

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LINC00473 exacerbates osteoarthritis development by promoting chondrocyte apoptosis and proinflammatory cytokine production through the miR-424-5p/LY6E axis.

Exp Ther Med 2021 Nov 2;22(5):1247. Epub 2021 Sep 2.

Department of Neonatology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China.

Osteoarthritis (OA) is a common degenerative joint disease that has been identified as one of the major health burdens in aging individuals. Long non-coding RNAs (lncRNAs) participate in the development of diverse diseases, including OA. Among them, lncRNA long intergenic non-protein coding RNA 473 (LINC00473) is one of the few upregulated lncRNAs. Read More

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November 2021

High Content Image Analysis as a Tool to Morphologically Distinguish Macrophage Activation and Determine Its Importance for Foamy Alveolar Macrophage Responses.

Front Immunol 2021 1;12:611280. Epub 2021 Sep 1.

Centre for Topical Drug Delivery and Toxicology School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom.

Introduction: Lung diseases are an increasing global health burden affecting millions of people worldwide. Only a few new inhaled medicines have reached the market in the last 30 years, in part due to foamy alveolar macrophage (FAM) responses observed in pre-clinical rat studies. The induction mechanism and signaling pathways involved in the development of highly vacuolated 'foamy' phenotype is not known. Read More

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September 2021

Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors.

Int Immunopharmacol 2021 Sep 17;100:108120. Epub 2021 Sep 17.

Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.

Aims: The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).

Methods And Results: In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Read More

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September 2021

A multi-locus approach to elucidating the evolutionary history of the clingfish Tomicodon petersii (Gobiesocidae) in the Tropical Eastern Pacific.

Mol Phylogenet Evol 2021 Sep 16:107316. Epub 2021 Sep 16.

Instituto Nacional de Biodiversidad (INABIO), Colección de Peces Calle Rumipamba 341, Av. De los Shyris, Parque "La Carolina", Quito, Ecuador; Laboratorio de Biología Acuática, Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Edificio "R" planta baja, Ciudad Universitaria, Morelia, Michoacán 58030, México. Electronic address:

Marine species that are widely distributed in the Tropical Eastern Pacific (TEP) has served as a model for studying biogeographic patterns resulting from the effects of intraregional habitat discontinuities and oceanographic processes on the diversification and evolution of cryptobenthic reef fishes. Tomicodon petersii, a clingfish (Gobiesocidae) endemic to the TEP, is found on very shallow rocky reefs from central Mexico to northern Peru, and in the Cocos and Galapagos islands. We evaluated the effect of likely biogeographic barriers in different parts of the TEP on the diversification process of this species. Read More

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September 2021

ASSESSMENT OF ENDOTHELIAL DYSFUNCTION IN PREGNANT WOMEN WITH OBESITY AND PREECLAMPSIA.

Wiad Lek 2021 ;74(8):1905-1909

POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE.

Objective: The aim: To assess the values of endothelial vascular growth factor (VEGF) in blood serum and circulating endothelial microparticles CD32+CD40+ in the peripheral blood of pregnant women depending on the severity of obesity and presence of preeclampsia.

Patients And Methods: Materials and methods: the study included 122 pregnant women divided into groups in accordance with their height and weight parameters and presence of preeclampsia. We studied the serum VEGF concentration by enzyme-linked immunosorbent assay, carried out the count of CD32+CD40+ circulating endothelial microparticles in the peripheral blood by using flow cytometry. Read More

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September 2021

Genomic DNA hydroxymethylation reveals potential role in identification of lung injury in coal-burning arsenicosis populations.

Environ Res 2021 Sep 15;204(Pt B):112053. Epub 2021 Sep 15.

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, PR China. Electronic address:

Arsenic (As) is a toxic metalloid element that causes lung cancer and multiple non-malignant respiratory diseases. The toxicity of arsenic is mediated in part by epigenetic mechanisms, such as alterations in DNA methylation. While increasing studies have highlighted the potential importance of arsenic exposure to DNA methylation patterns and the subsequent risks for arsenic toxicity, there has been little focus on DNA hydroxymethylation-a negative regulation mechanism of DNA methylation. Read More

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September 2021

Cytokine profile in Egyptian children and adolescents with COVID-19 pneumonia: a multicenter study.

Pediatr Pulmonol 2021 Sep 18. Epub 2021 Sep 18.

Department of Radiology, Texas Tech University Health Sciences Center El Paso, Texas, USA.

Background: To date, the cytokine profile in children and adolescent with novel coronavirus disease 2019 (COVID-19) has not been reported.

Objectives: we investigated serum levels of a panel of key cytokines in children and adolescent with COVID-19 pneumonia with a primary focus on "cytokine storm" cytokines such as IL-1β, IL-6, IL-17, IL-2, IL-4, IL-10, interferon (IFN-γ), tumor necrosis factor (TNF)-α and two chemokines interferon inducible protein-10 (IP-10) and IL-8]. We also studied whether these cytokines could be potential markers for illness severity in COVID-19 pneumonia. Read More

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September 2021

Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes.

J Cell Mol Med 2021 Sep 18. Epub 2021 Sep 18.

Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.

Mesenchymal stem/stromal cells isolated from human term placenta (pMSCs) have potential to treat clinically manifested inflammatory diseases. Atherosclerosis is a chronic inflammatory disease, and platelets play a contributory role towards its pathogenesis. During transplantation, MSCs interact with platelets and exert influence on their functional outcome. Read More

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September 2021

Effects of Hydroxychloroquine on endOthelial function in eLDerly with sleep apnea (HOLD): study protocol for a randomized clinical trial.

Trials 2021 Sep 17;22(1):638. Epub 2021 Sep 17.

Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Background: Sleep apnea and coronary artery disease are prevalent and relevant diseases. The mechanism by which sleep apnea leads to coronary artery disease remains unclear. Intermittent hypoxia, caused by sleep apnea, leads to inflammation and consequent endothelial dysfunction. Read More

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September 2021

, negatively regulated by miR-128-3p, promotes the progression of non-small-cell lung cancer.

Per Med 2021 Sep 17. Epub 2021 Sep 17.

Department of Thoracic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 201308, China.

To investigate the effects of SKA3 on cell proliferation and metastasis in non-small-cell lung cancer (NSCLC) and its underlying mechanism. Immunohistochemistry was employed to analyze the expression of SKA3 in NSCLC. CCK-8 assay, EdU assay, Transwell assay and flow cytometry analysis were employed to assess cell proliferation, metastatic potential and apoptosis , respectively. Read More

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September 2021

Mesenchymal stem cell-derived exosomes regulate the polarization and inflammatory response of macrophages via miR-21-5p to promote repair after myocardial reperfusion injury.

Authors:
Dafu Shen Zhiwei He

Ann Transl Med 2021 Aug;9(16):1323

Department of General Surgery, Shanghai Post and Telecommunication Hospital, Shanghai, China.

Background: Myocardial ischemia-reperfusion injury is a type of myocardial ischemia that has a significant impact on patients' health. We aimed to explore the protective effect of mesenchymal stem cell-derived exosomes (MSC-EXOs) on myocardial ischemia-reperfusion injury and their specific mechanism.

Methods: The effects of MSC-EXOs on myocardial ischemia-reperfusion injury were recorded. Read More

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Oxidative stress-induced impairment of trophoblast function causes preeclampsia through the unfolded protein response pathway.

Sci Rep 2021 Sep 16;11(1):18415. Epub 2021 Sep 16.

Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.

Pre-eclampsia (PE) is a pregnancy-specific disorder, characterized by hypertension and proteinuria. In PE, trophoblasts mediated inadequate remodeling of uterine spiral arteries seem to interrupt uteroplacental blood flow, one of the hallmarks in the early onset of PE (EO-PE). This, in turn, results in placental ischemia-reperfusion injury during hypoxia and reoxygenation episodes, leading to the generation of reactive oxygen species (ROS) and oxidative stress (OS). Read More

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September 2021

Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes.

J Immunother Cancer 2021 Sep;9(9)

Immunology, Okayama University of Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan

Background: Metformin (Met) is the first-line treatment for type 2 diabetes mellitus and plays an effective role in treating various diseases, such as cardiovascular disease, neurodegenerative disease, cancer, and aging. However, the underlying mechanism of Met-dependent antitumor immunity remains to be elucidated.

Methods: MitoTEMPO, a scavenger of mitochondrial superoxide, abolished the antitumor effect of Met, but not antiprogrammed cell death (PD-1) antibody (Ab) treatment. Read More

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September 2021

Germline and Somatic Variants Convergently Promote Endothelial-to-Mesenchymal Transition in Simplex Brain Arteriovenous Malformation.

Circ Res 2021 Sep 17. Epub 2021 Sep 17.

Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, CHINA.

Brain arteriovenous malformations (bAVMs) are abnormal entanglement of blood vessels in brain, with direct connections from arteries to veins, lacking functional capillary bed. Although several somatic mutations were reported, the molecular mechanism and genetic disposition of bAVM remain poorly understood. We aim to identify transcriptional anomalies and critical functional pathways in bAVM lesions, and explore their association with key de novo germline and somatic variants in bAVM patients. Read More

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September 2021

[CacyBP promotes the proliferation and invasion of non-small cell lung cancer].

Zhonghua Zhong Liu Za Zhi 2021 Sep;43(9):924-931

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

To investigate the effects and the mechanism of Calcyclin-binding protein (CacyBP) on the proliferation and invasion of non-small cell lung cancer (NSCLC) cells. Six lung cancer tissues and paired normal lung tissues were collected from NSCLC patients who underwent surgical treatment in Jinan Central Hospital during 2016. The expression of CacyBP in these tissues was examined by western blot. Read More

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September 2021

Protective effects of 17β-oestradiol on coagulation and systemic inflammation after total occlusion of the descending aorta in male rats.

Eur J Cardiothorac Surg 2021 Sep 16. Epub 2021 Sep 16.

Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.

Objectives: The surgical treatment for diseases of the descending aorta is related to a high mortality rate because of the activation of a systemic inflammatory process due to ischaemia and reperfusion (I/R) injury. Activation of coagulation can contribute to the inflammatory process, resulting in microcirculatory damage and multiple organ failure. Our goal was to evaluate the role of prophylactic intravenous 17β-oestradiol (E2) in coagulation, the inflammatory response and hepatic injury after occlusion of the descendent proximal aorta in male rats. Read More

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September 2021

Pulmonary vascular disease in Fontan circulation-is there a rationale for pulmonary vasodilator therapies?

Cardiovasc Diagn Ther 2021 Aug;11(4):1111-1121

Department of Congenital Heart Disease and Pediatric Cardiology, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany.

The Fontan circulation is a palliative concept for patients with univentricular hearts. The central veins are connected directly to the pulmonary arteries (cavo-pulmonary connection) to separate the pulmonary and the systemic circulation. There is no sub-pulmonary ventricle that generates pressure to drive blood through the pulmonary arteries. Read More

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Stromal infiltrating mast cells identify immunoevasive subtype high-grade serous ovarian cancer with poor prognosis and inferior immunotherapeutic response.

Oncoimmunology 2021 11;10(1):1969075. Epub 2021 Sep 11.

Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.

Tumor infiltrating mast cells (TIMs), with pro- or anti-tumorigenic role in different types of malignancies, have been implicated in resistance to anti-PD1 therapy. Here, we aimed to identify the relevance of TIMs with the prognosis, immune contexture, and immunotherapy in high-grade serous ovarian cancer (HGSOC). Tissue microarrays containing 197 HGSOC patients were assessed by immunohistochemistry (IHC) for detecting the expression of mast cell tryptase and other immune markers. Read More

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September 2021