J Mol Diagn 2021 May 31. Epub 2021 May 31.
Department of Internal Medicine - Hematology and Oncology, Faculty of Medicine, Masaryk University and University Hospital Brno, Czech Republic; Center of Molecular Medicine, CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Department of Medical Genetics and Genomics, Faculty of Medicine, Masaryk University and University Hospital Brno, Czech Republic. Electronic address:
B-cell neoplasms represent a clinically heterogeneous group of hematological malignancies with considerably diverse genomic architecture recently endorsed by next-generation sequencing (NGS) studies. Since multiple genetic defects have potential or confirmed clinical impact, a tendency toward more comprehensive testing of diagnostic, prognostic, and predictive markers is desired. In this study, we introduce the design, validation, and implementation of an integrative custom-designed capture-based NGS panel titled LYNX (LYmphoid NeXt-generation sequencing) for the analysis of standard and novel molecular markers in the most common lymphoid malignancies (chronic lymphocytic leukemia, acute lymphoblastic leukemia, diffuse large B-cell lymphoma, follicular lymphoma, mantle cell lymphoma). Read More