21,052 results match your criteria macrophages ability

IL-10 and TGF-β cytokines decrease immune activation during normothermic ex vivo machine perfusion of the rat liver.

Liver Transpl 2021 Jun 12. Epub 2021 Jun 12.

Department of Surgery, Division of Transplantation, University of Wisconsin, School of Medicine and Public Health, Madison.

Background: Normothermic ex vivo liver perfusion (NEVLP) is a novel system for organ preservation which may improve over static cold storage (CS) clinically and offers the chance for graft modification prior to transplantation. Although recent studies have shown the presence of inflammatory molecules during perfusion, none have yet shown the effects of NEVLP on liver-resident immune cell activation. We investigated the effects of NEVLP on liver-resident immune cell activation and assessed the ability of anti-inflammatory cytokines IL-10 and TGF-β to improve organ function and reduce immune activation during perfusion. Read More

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Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas.

Front Endocrinol (Lausanne) 2021 25;12:606175. Epub 2021 May 25.

Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.

The tissue microenvironment in the mouse pancreas has been shown to promote very different polarizations of resident macrophages with islet-resident macrophages displaying an inflammatory "M1" profile and macrophages in the exocrine tissue mostly displaying an alternatively activated "M2" profile. The impact of this polarization on tissue homeostasis and diabetes development is unclear. In this study, the ability of pancreas-resident macrophages to phagocyte bacterial and endogenous debris was investigated. Read More

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Macrophage biomimetic nanocarriers for anti-inflammation and targeted antiviral treatment in COVID-19.

J Nanobiotechnology 2021 Jun 10;19(1):173. Epub 2021 Jun 10.

Center for Infection and Immunity, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, 519000, Guangdong, China.

Background: The worldwide pandemic of COVID-19 remains a serious public health menace as the lack of efficacious treatments. Cytokine storm syndrome (CSS) characterized with elevated inflammation and multi-organs failure is closely correlated with the bad outcome of COVID-19. Hence, inhibit the process of CSS by controlling excessive inflammation is considered one of the most promising ways for COVID-19 treatment. Read More

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Identification of genes required for the fitness of Rhodococcus equi during the infection of mice via signature-tagged transposon mutagenesis.

J Vet Med Sci 2021 Jun 10. Epub 2021 Jun 10.

Laboratory of Animal Hygiene, School of Veterinary Medicine, Kitasato University.

Rhodococcus equi is a Gram-positive facultative intracellular bacterium that causes pyogranulomatous pneumonia in foals and immunocompromised people. In the present study, signature-tagged transposon mutagenesis was applied for the negative selection of R. equi mutants that cannot survive in vivo. Read More

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Extracellular Acidity Reprograms Macrophage Metabolism and Innate Responsiveness.

J Immunol 2021 Jun 9. Epub 2021 Jun 9.

Department of Immunology, Key Laboratory of Medical Molecular Virology (MOE, NHC, CAMS), School of Basic Medical Sciences, Fudan University, Shanghai, China;

Although organ hypofunction and immunosuppression are life-threatening features of severe sepsis, the hypofunctioning organs and immune cells usually regain normal functionality if patients survive. Because tissue interstitial fluid can become acidic during the septic response, we tested the hypothesis that low extracellular pH (pHe) can induce reversible metabolic and functional changes in peritoneal macrophages from C57BL/6J mice. When compared with macrophages cultured at normal pHe, macrophages living in an acidic medium used less glucose and exogenous fatty acid to produce ATP. Read More

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Zoonotic vaccinia virus strains belonging to different genetic clades exhibit immunomodulation abilities that are proportional to their virulence.

Virol J 2021 Jun 9;18(1):124. Epub 2021 Jun 9.

Laboratory of Basic and Applied Virology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

Background: The vaccinia virus (VACV) isolates, Guarani P1 virus (GP1V) and Passatempo virus (PSTV), were isolated during zoonotic outbreaks in Brazil. Each one of them belongs to two different VACV clades, defined by biological aspects that include virulence in mice and phylogenetic analysis. Considering that information about how vaccinia viruses from different groups elicit immune responses in animals is scarce, we investigated such responses in mice infected either by GP1V (group 2) or PSTV (group 1), using VACV Western Reserve strain (VACV-WR) as control. Read More

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Identification of an Alveolar Macrophage-Related Core Gene Set in Acute Respiratory Distress Syndrome.

J Inflamm Res 2021 1;14:2353-2361. Epub 2021 Jun 1.

Department of Emergency Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530007, People's Republic of China.

Purpose: Acute respiratory distress syndrome (ARDS) is a rapidly progressive diffuse lung injury that is characterized by high mortality and acute onset. The pathological mechanisms of ARDS are still unclear. But alveolar macrophages have been shown to play an important role in inflammatory responses during ARDS. Read More

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AXL inhibition in macrophages stimulates host-versus-leukemia immunity and eradicates naive and treatment resistant leukemia.

Cancer Discov 2021 Jun 8. Epub 2021 Jun 8.

Med. Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus.

Acute leukemias are systemic malignancies associated with a dire outcome. Due to low immunogenicity, leukemias display a remarkable ability to evade immune control and are often resistant to checkpoint blockade. Here, we discover that leukemia cells actively establish a suppressive environment to prevent immune attacks by co-opting a signaling axis that skews macrophages towards a tumor promoting tissue repair phenotype, namely the GAS6/AXL axis. Read More

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Boosting immune system against cancer by resveratrol.

Phytother Res 2021 Jun 8. Epub 2021 Jun 8.

Department of Medical Physics, Tehran University of Medical Sciences, Tehran, Iran.

Modulation of the immune system is a critical part of anticancer therapies including immunotherapy, chemotherapy, and radiotherapy. The aim of immunomodulation in cancer therapy is boosting immune system cells including CD8+ T lymphocytes and natural killer (NK) cells, as well as suppression of immunosuppressive responses by macrophages and regulatory T cells (Tregs). Usually, using single or dual modality can induce immune system responses against cancer. Read More

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Redox modifications of cysteine residues regulate the cytokine activity of HMGB1.

Mol Med 2021 Jun 7;27(1):58. Epub 2021 Jun 7.

Laboratory of Biomedical Science, The Feinstein Institute for Medical Research, Manhasset, NY, USA.

Background: High mobility group box 1 (HMGB1) is a nuclear protein with extracellular inflammatory cytokine activity. It is passively released during cell death and secreted by activated cells of many lineages. HMGB1 contains three conserved redox-sensitive cysteine residues: cysteines in position 23 and 45 (C23 and C45) can form an intramolecular disulfide bond, whereas C106 is unpaired and is essential for the interaction with Toll-Like Receptor (TLR) 4. Read More

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Typhimurium adhesin OmpV activates host-immunity to confer protection against systemic and gastro-intestinal infection in mice.

Infect Immun 2021 Jun 7:IAI0012121. Epub 2021 Jun 7.

Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Sector 81, SAS Nagar, Mohali, Manauli, Punjab 140306, India.

Typhimurium is a rod-shaped gram-negative bacterium that mostly enters the human body through contaminated food. It causes gastro-intestinal disorder called salmonellosis in humans and typhoid like systemic disease in mice. OmpV, an outer-membrane protein of Typhimurium helps in adhesion and invasion of bacteria to intestinal epithelial cells and thus play a vital role in the pathogenesis of Typhimurium. Read More

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Development of a compound oral liquid containing herbal extracts and its effect on immunity and gastric mucosa.

J Food Sci 2021 Jun 6. Epub 2021 Jun 6.

State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy and Food Company Ltd., Shanghai, China.

Nowadays, consumers have an increasing demand for health products. In this study, an oral liquid was developed using a compound extract consisting of three herbal extracts (Dendrobium nobile Lindl., Lycium barbarum, and Puerariae lobatae Radix) because the compound extract (a combination of all three extracts) was superior to every single extract in promoting the phagocytic capacity of RAW264. Read More

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Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for lectin (BambL).

Chem Sci 2020 Oct 21;11(47):12662-12670. Epub 2020 Oct 21.

Università di Firenze, Department of Chemistry via della Lastruccia, 3, 13, 50019 Sesto F.no FI Italy

Two orthogonal, metal free click reactions, enabled to glycosylate ubiquitin and its mutant A28C forming two protein scaffolds with high affinity for BambL, a lectin from the human pathogen . A new fucoside analogue, with high affinity with BambL, firstly synthetized and co-crystallized with the protein target, provided the insights for sugar determinants grafting onto ubiquitin. Three ubiquitin-based glycosides were thus assembled. Read More

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October 2020

An innovative NRF2 nano-modulator induces lung cancer ferroptosis and elicits an immunostimulatory tumor microenvironment.

Theranostics 2021 13;11(14):7072-7091. Epub 2021 May 13.

Department of Pharmacology, National Cheng Kung University, Tainan, 70101, Taiwan.

Simultaneous targeting of both the tumor microenvironment and cancer cells by a single nanomedicine has not been reported to date. Here, we report the dual properties of zero-valent-iron nanoparticle (ZVI-NP) to induce cancer-specific cytotoxicity and anti-cancer immunity. Cancer-specific cytotoxicity induced by ZVI-NP was determined by MTT assay. Read More

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Bi-directional regulation functions of lanthanum-substituted layered double hydroxide nanohybrid scaffolds activating osteogenesis and inhibiting osteoclastogenesis for osteoporotic bone regeneration.

Theranostics 2021 3;11(14):6717-6734. Epub 2021 May 3.

The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.

Osteoporotic patients suffer symptoms of excessive osteoclastogenesis and impaired osteogenesis, resulting in a great challenge to treat osteoporosis-related bone defects. Based on the positive effect of rare earth elements on bone metabolism and bone regeneration, we try to prove the hypothesis that the La dopants in lanthanum-substituted MgAl layered double hydroxide (La-LDH) nanohybrid scaffolds simultaneously activate osteogenesis and inhibit osteoclastogenesis. A freeze-drying technology was employed to construct La-LDH nanohybrid scaffolds. Read More

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Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis.

Biochim Biophys Acta Mol Basis Dis 2021 Jun 1;1867(10):166184. Epub 2021 Jun 1.

Jiangsu Provincial Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Science, Soochow University, Suzhou 215123, People's Republic of China. Electronic address:

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with unclear pathogenesis. We previously reported that syngenetic, activated lymphocyte-derived DNA (ALD-DNA) could robustly elicit macrophage activation, which plays an important role in the pathogenesis of murine lupus nephritis. In addition, extracellular HMGB1 obviously facilitated the accumulation of ALD-DNA in endosomes and promoted macrophage inflammation. Read More

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No Small Matter: emerging roles for exosomal miRNAs in the immune system.

FEBS J 2021 Jun 4. Epub 2021 Jun 4.

Divison of Microbiology and Immunology, Department of Pathology, University of Utah, Salt Lake City, UT, 84112, USA.

Extracellular communication is critical to the function of an organism. Exosomes, small lipid vesicles, have been recently appreciated to participate in this vital function. Within these vesicles lie critical bioactive molecules including mRNAs, proteins and a plethora of non-coding RNAs, including microRNAs (miRNAs). Read More

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Baicalin-loaded macrophage-derived exosomes ameliorate ischemic brain injury via the antioxidative pathway.

Mater Sci Eng C Mater Biol Appl 2021 Jul 23;126:112123. Epub 2021 Apr 23.

School of Pharmacy, Jinzhou Medical University, Jinzhou 121000, PR China. Electronic address:

Baicalin (BA), a strong free radical scavenger, has been demonstrated to exert neuroprotective effects in the treatment of ischemic stroke. However, its clinical application has been limited due to its inability to target the brain and its poor solubility. In this study, we designed novel brain-targeted BA-loaded macrophage-derived exosomes (Exo-BA) to induce neuroprotection against ischemic stroke in animal models. Read More

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NHS-IL12, a Tumor-Targeting Immunocytokine.

Immunotargets Ther 2021 27;10:155-169. Epub 2021 May 27.

Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

NHS-IL12 is a novel immunocytokine designed for delivery of IL-12 to the tumor microenvironment (TME). NHS-IL12 consists of two molecules of IL-12 fused to a human IgG1 (NHS76) recognizing DNA/histone complexes, which are often exposed in the necrotic portions of tumors. Preclinical studies demonstrated the tumor-targeting ability and longer plasma half-life for NHS-IL12 when compared with recombinant IL-12 (rIL-12). Read More

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Harmine Alleviates Titanium Particle-Induced Inflammatory Bone Destruction by Immunomodulatory Effect on the Macrophage Polarization and Subsequent Osteogenic Differentiation.

Front Immunol 2021 17;12:657687. Epub 2021 May 17.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, China.

Peri-prosthetic osteolysis (PPO) and following aseptic loosening are regarded as the prime reasons for implant failure after joint replacement. Increasing evidence indicated that wear-debris-irritated inflammatory response and macrophage polarization state play essential roles in this osteolytic process. Harmine, a β-carboline alkaloid primitively extracted from the seeds, has been reported to have various pharmacological effects on monoamine oxidase action, insulin intake, vasodilatation and central nervous systems. Read More

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Systemic blockade of Clever-1 elicits lymphocyte activation alongside checkpoint molecule downregulation in patients with solid tumors: Results from a phase I/II clinical trial.

Clin Cancer Res 2021 Jun 2. Epub 2021 Jun 2.

MediCity, University of Turku

Purpose: Macrophages are critical in driving an immunosuppressive tumor microenvironment that counteracts the efficacy of T-cell-targeting therapies. Thus, agents able to reprogram macrophages towards a proinflammatory state hold promise as novel immunotherapies for solid cancers. Inhibition of a macrophage scavenger receptor Clever-1 has shown benefit in inducing CD8 T-cell-mediated antitumor responses in mouse models of cancer, which supports the clinical development of Clever-1-targeting antibodies for cancer treatment. Read More

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Group B Is Required for Serotype V Capsule Production and Aids in Biofilm Formation and Ascending Infection of the Reproductive Tract during Pregnancy.

ACS Infect Dis 2021 Jun 2. Epub 2021 Jun 2.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37212, United States.

Group B (GBS) is an encapsulated Gram-positive pathogen that causes ascending infections of the reproductive tract during pregnancy. The capsule of this organism is a critical virulence factor that has been implicated in a variety of cellular processes to promote pathogenesis. Primarily comprised of carbohydrates, the GBS capsule and its synthesis is driven by the apsule olysaccharide ynthesis () operon. Read More

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HPV16 infection promotes an M2 macrophage phenotype to promote the invasion and metastasis of esophageal squamous cell carcinoma.

Clin Transl Oncol 2021 Jun 1. Epub 2021 Jun 1.

Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases (Ministry of Education), The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi , Xinjiang , 832000, China.

Objectives: High-risk human papillomavirus (HR-HPV) is an important risk factor for esophageal cancer. Macrophages constitute a crucial immune medium for regulating HPV-related tumors; however, the specific regulatory mechanisms remain unknown. Therefore, the purpose of our current study was to investigate the mechanism by which HPV16E6 regulates macrophages to promote the invasion and metastasis of esophageal cancer. Read More

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Gold Nanoparticles and Graphene Oxide Flakes Enhance Cancer Cells' Phagocytosis through Granzyme-Perforin-Dependent Biomechanism.

Nanomaterials (Basel) 2021 May 24;11(6). Epub 2021 May 24.

Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia.

The study aimed to investigate the roles of gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) as phagocytosis enhancers against cancer cells. The nanomaterials were characterized through SEM and UV-VIS absorptions. The GNPs and GOFs increased the macrophages' phagocytosis ability in engulfing, thereby annihilating the cancer cells in both in vitro and in vivo conditions. Read More

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SR-B1, a Key Receptor Involved in the Progression of Cardiovascular Disease: A Perspective from Mice and Human Genetic Studies.

Biomedicines 2021 May 27;9(6). Epub 2021 May 27.

Unidad Clínica y de Investigación en Lípidos y Arteriosclerosis, Instituto de Investigación Sanitaria Aragón (IIS Aragón), Hospital Universitario Miguel Servet, 50009 Zaragoza, Spain.

High plasma level of low-density lipoprotein (LDL) is the main driver of the initiation and progression of cardiovascular disease (CVD). Nevertheless, high-density lipoprotein (HDL) is considered an anti-atherogenic lipoprotein due to its role in reverse cholesterol transport and its ability to receive cholesterol that effluxes from macrophages in the artery wall. The scavenger receptor B class type 1 (SR-B1) was identified as the high-affinity HDL receptor, which facilitates the selective uptake of cholesterol ester (CE) into the liver via HDL and is also implicated in the plasma clearance of LDL, very low-density lipoprotein (VLDL) and lipoprotein(a) (Lp(a)). Read More

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Renal Cell Carcinoma-Infiltrating CD3 Vγ9Vδ1 T Cells Represent Potentially Novel Anti-Tumor Immune Players.

Curr Issues Mol Biol 2021 May 27;43(1):226-239. Epub 2021 May 27.

Samsung Medical Center, Department of Urology, Sungkyunkwan University School of Medicine, Seoul 06351, Korea.

Due to the highly immunogenic nature of renal cell carcinoma (RCC), the tumor microenvironment (TME) is enriched with various innate and adaptive immune subsets. In particular, gamma-delta (γδ) T cells can act as potent attractive mediators of adoptive cell transfer immunotherapy because of their unique properties such as non-reliance on major histocompatibility complex expression, their ability to infiltrate human tumors and recognize tumor antigens, relative insensitivity to immune checkpoint molecules, and broad tumor cytotoxicity. Therefore, it is now critical to better characterize human γδ T-cell subsets and their mechanisms in RCCs, especially the stage of differentiation. Read More

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Evaluation of the Photodynamic Therapy with Curcumin on and Amastigotes.

Antibiotics (Basel) 2021 May 25;10(6). Epub 2021 May 25.

Photobios-Photobiology Applied to Health, University of Vale do Paraíba, Urbanova, São José dos Campos 2911, SP, Brazil.

Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with and to PDT with curcumin. Read More

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The Effect of Inflammatory Priming on the Therapeutic Potential of Mesenchymal Stromal Cells for Spinal Cord Repair.

Cells 2021 May 25;10(6). Epub 2021 May 25.

Biologics Lab, Shirley Ryan Ability Lab, Chicago, IL 60611, USA.

Mesenchymal stromal cells (MSC) are used for cell therapy for spinal cord injury (SCI) because of their ability to support tissue repair by paracrine signaling. Preclinical and clinical research testing MSC transplants for SCI have revealed limited success, which warrants the exploration of strategies to improve their therapeutic efficacy. MSC are sensitive to the microenvironment and their secretome can be altered in vitro by exposure to different culture media. Read More

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Pivotal Role for Cxcr2 in Regulating Tumor-Associated Neutrophil in Breast Cancer.

Cancers (Basel) 2021 May 25;13(11). Epub 2021 May 25.

CNRS, SYS2DIAG-ALCEDIAG, Cap Delta, 1682 rue de la Valsière, 34184 Montpellier, France.

Chemokines present in the tumor microenvironment are essential for the control of tumor progression. We show here that several ligands of the chemokine receptor Cxcr2 were up-regulated in the PyMT (polyoma middle T oncogene) model of breast cancer. Interestingly, the knock-down of Cxcr2 in PyMT animals led to an increased growth of the primary tumor and lung metastasis. Read More

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Folate Receptor Beta as a Direct and Indirect Target for Antibody-Based Cancer Immunotherapy.

Int J Mol Sci 2021 May 25;22(11). Epub 2021 May 25.

Ovarian Cancer Research Center, Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Folate receptor beta (FRβ) is a folate binding receptor expressed on myeloid lineage hematopoietic cells. FRβ is commonly expressed at high levels on malignant blasts in patients with acute myeloid leukemia (AML), as well as on M2 polarized tumor-associated macrophages (TAMs) in the tumor microenvironment of many solid tumors. Therefore, FRβ is a potential target for both direct and indirect cancer therapy. Read More

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