Biomed Environ Sci 2021 Sep;34(9):683-692
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Center for Global Public Health, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, Hubei China;China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective: To find the different electrophoretic profiles of prion protein in carcinous and individual pericarcinous tissues in lysates of gastric, colon, liver, lung, thyroid, and laryngeal cancers.
Methods: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot were used to test the amounts and electrophoretic patterns of total PrP and the tolerance of PK (protease K) digestion among six various cancer tissue types.
Results: A mass of PrP signals with a large molecular weight were identified in the homogenates of peripheral tissues. Read More