1,065 results match your criteria lsr


A one-step deconstruction-separation organosolv fractionation of lignocellulosic biomass using acetone/phenoxyethanol/water ternary solvent system.

Bioresour Technol 2021 Dec 20;342:125963. Epub 2021 Sep 20.

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address:

A novel ternary solvent system for organosolv fractionation of lignocellulosic biomass, named APW process, which is composed of acetone, phenoxyethanol and water with the advantages of monophasic deconstruction and biphasic separation of components was developed. Through fractionation of amorpha as a case study, a monophasic APW solution (acetone/phenoxyethanol/water = 5:11:4, volume ratio) with the best lignin affinity was constructed based on Hansen solubility parameters. According to Taguchi experimental design, the optimal conditions were 130 °C, 70 min, 0. Read More

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December 2021

Construction of an AI-2 quorum sensing induced heterologous protein expression system in .

PeerJ 2021 16;9:e12497. Epub 2021 Nov 16.

Anhui Agricultural University, School of Life Sciences, Hefei, Anhui, China.

Background: The pET expression system based on T7 promoter which is induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) is by far the most commonly used system for production of heterogeneous proteins in . However, this system was limited by obvious drawbacks including the host toxicity and metabolic burden imposed by the presence of IPTG.

Methods: In this study, we incorporated the autoinducer-2 (AI-2) quorum sensing system to realize autoinduction of the pET expression system. Read More

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November 2021

Tracheal intubation in the critically ill patient.

Eur J Anaesthesiol 2021 Nov 18. Epub 2021 Nov 18.

From the Department of Anesthesia and Intensive Care, University Hospital San Luigi Gonzaga, University of Turin, Italy (VR), Department of Emergency and Intensive Care, University Hospital San Gerardo, Monza (GB), University of Milano-Bicocca, Milan, Italy (GB), Department of Anaesthesiology, Critical Care and Pain Medicine, Children's Health Ireland at Temple Street, Dublin, Ireland (LSR), Department of Anesthesiology and Pain Medicine; Interdepartmental Division of Critical Care Medicine, University of Toronto (MP), Department of Anesthesia and Pain Management, Toronto General Hospital, Toronto, Canada (MP), Regenerative Medicine Institute at CURAM Centre for Medical Devices, School of Medicine, National University of Ireland (JGL) and Anaesthesia and Intensive Care Medicine, University Hospital Galway, Galway, Ireland (JGL).

Tracheal intubation is among the most commonly performed and high-risk procedures in critical care. Indeed, 45% of patients undergoing intubation experience at least one major peri-intubation adverse event, with cardiovascular instability being the most common event reported in 43%, followed by severe hypoxemia in 9% and cardiac arrest in 3% of cases. These peri-intubation adverse events may expose patients to a higher risk of 28-day mortality, and they are more frequently observed with an increasing number of attempts to secure the airway. Read More

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November 2021

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

J Hazard Mater 2021 Oct 20;424(Pt C):127548. Epub 2021 Oct 20.

Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Arsenic (As), a widespread environmental contaminant, can induce serious male reproductive injury; however, the underlying mechanisms remain unclear. Multi-omics analyses, including transcriptome, proteome, and phosphoproteome could promote our understanding of As-induced male reproductive toxicity. Here, we established the reproductive injured mice model by intraperitoneal injection of NaAsO2 (8 mg/kg body weight), which was validated by reduced reproductive cells, sperm motility, and litter size. Read More

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October 2021

CRISPA: A Non-viral, Transient Cas9 Delivery System Based on Reengineered Anthrax Toxin.

Front Pharmacol 2021 18;12:770283. Epub 2021 Oct 18.

Institute of Pharmacology and Toxicology, Ulm University Medical Center, Ulm, Germany.

Translating the CRISPR/Cas9 genome editing technology into clinics is still hampered by rather unspecific, unsafe and/or inconvenient approaches for the delivery of its main components - the Cas9 endonuclease and a guide RNA - into cells. Here, we describe the development of a novel transient and non-viral Cas9 delivery strategy based on the translocation machinery of the anthrax toxin, PA (protective antigen). We show that Cas9 variants fused to the N-terminus of the lethal factor or to a hexahistidine tag are shuttled through channels formed by PA into the cytosol of human cells. Read More

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October 2021

Evaluation of the Stability of a Novel Lateral Plate Internal Fixation: An In Vitro Biomechanical Study.

World Neurosurg 2021 Oct 30. Epub 2021 Oct 30.

Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. Electronic address:

Background: This study aims to evaluate the biomechanical stability of a novel lateral plate (NLP) that can be used in oblique lateral lumbar fusion (OLIF).

Methods: In vitro biomechanical tests were performed on 6 fresh calf lumbar vertebrae specimens. The surgical segment was set at L3-L4. Read More

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October 2021

The Nonoperative Management of Minimally Displaced Lateral Compression Type 1 (LC1) Pelvic Ring Injuries With and Without Occult Instability.

J Orthop Trauma 2021 Oct 20. Epub 2021 Oct 20.

Department of Orthopaedics, Denver Health Medical Center, University of Colorado School of Medicine, Denver, Colorado.

Objectives: To determine the association of pelvic fracture displacement on lateral stress radiographs (LSR) with the hospital course of patients with minimally displaced lateral compression type 1 (LC1) pelvic injuries.

Design: Retrospective review.

Setting: Level one trauma centerPatients/Participants: 28 adult patients with minimally displaced (<1cm) LC1 injuries. Read More

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October 2021

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes.

Materials (Basel) 2021 Oct 11;14(20). Epub 2021 Oct 11.

Department of Tropical Medicine and Infectious Diseases, Center of Internal Medicine II, University Medical Center Rostock, 18057 Rostock, Germany.

The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host's immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. Read More

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October 2021

Species interactions, environmental gradients and body size shape population niche width.

J Anim Ecol 2021 Oct 17. Epub 2021 Oct 17.

Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.

Competition for shared resources is commonly assumed to restrict population-level niche width of coexisting species. However, the identity and abundance of coexisting species, the prevailing environmental conditions, and the individual body size may shape the effects of interspecific interactions on species' niche width. Here we study the effects of interspecific and intraspecific interactions, lake area and altitude, and fish body size on the trophic niche width and resource use of a generalist predator, the littoral-dwelling large, sparsely rakered morph of European whitefish (Coregonus lavaretus; hereafter LSR whitefish). Read More

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October 2021

Association of whole blood viscosity with thrombus presence in patients undergoing transoesophageal echocardiography.

Int J Cardiovasc Imaging 2021 Oct 15. Epub 2021 Oct 15.

Department of Cardiology, Sultan II. Abdülhamid Han Training and Research Hospital, Health Sciences University, Tibbiye Street, 34678, Üsküdar, Istanbul, Turkey.

Whole blood viscosity (WBV) is considered as a reasonable proxy measure of blood flow, and it has been investigated in different cohort settings, including in patients with deep venous thrombosis, arterial thrombosis, acute stent thrombosis, and left ventricular apical thrombus formation following acute coronary syndrome. To determine the association between WBV and the presence of thrombus in the left atrium (LA) or left atrial appendage (LAA) in individuals who had transoesophageal echocardiography (TEE). The clinical data from 262 consecutive patients who had TEE at our facility were included in this retrospective cohort study. Read More

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October 2021

Coronal knee alignment measurements differ on long-standing radiographs vs. by navigation.

Orthop Traumatol Surg Res 2021 Oct 11:103112. Epub 2021 Oct 11.

Centre hospitalier de Versailles, Le Chesnay, France. Electronic address:

Background: The long-standing radiograph (LSR) is the reference tool for assessing knee alignment after total knee arthroplasty (TKA). However, its value is debatable, as many factors can influence measurement accuracy. Computer-assisted surgery (CAS) provides accurate measurements. Read More

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October 2021

Do Hospital Workers Feel They Are Ready to Manage a Sanitary Crisis in a Pre-Crisis Context?

Disaster Med Public Health Prep 2021 Oct 14:1-7. Epub 2021 Oct 14.

LSR - Laboratoire des Sciences du Risque (Risk Science Laboratory), IMT Mines Alès, Alès, France.

Objective: The aim of this study was to measure the perception of readiness to manage a sanitary crisis for hospital workers and to study the factors related to this perception.

Methods: This study was a cross-sectional study; 408 French hospital workers responded to an online questionnaire. The variables studied concerned the perceived personal preparedness, the perception of colleagues' and hospital's preparedness, perception of the situation, and preparatory behavioral acts. Read More

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October 2021

Hatchability and growth performances of normal feathered local, Sasso-RIR and their F1-cross chickens managed under on-station condition in southern Ethiopia.

Trop Anim Health Prod 2021 Oct 13;53(5):513. Epub 2021 Oct 13.

Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia.

The Normal feathered local chicken (LL), Sasso-RIR (SRSR) and their F1-cross (LSR) chickens were hatched to evaluate for egg hatchability, body weight, feed efficiency, and survival rate. After 14 days of brooding, 150 chicks of each genotype were randomly selected and further replicated into five pens in a deep litter grower house consisting of 30 chicks each in a completely randomized design, and evaluated for a period of 16 weeks. Hatchability of fertile eggs was highest for LL (80. Read More

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October 2021

Undergraduate entomology field excursions are a valuable source of biodiversity data: a case for spider (Araneae) bycatches in ecological studies.

Biodivers Conserv 2021 Oct 7:1-24. Epub 2021 Oct 7.

Department of Zoology & Entomology, University of the Free State, P.O. Box 339, Bloemfontein, 9300 South Africa.

As part of an undergraduate Entomology module, field excursions were undertaken to a mixed livestock farm in central South Africa, during March-April 2015, 2016 and 2018-2020. The aim was for groups to determine and compare terrestrial arthropod biodiversity in three strata of three contrasting biotopes, with particular emphasis on insects. To determine the contributions such excursions make to documenting biodiversity of a non-target taxon, the spider (Arachnida: Araneae) data generated by students was compared with the local species richness (LSR) for the area. Read More

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October 2021

Living Systematic Reviews.

Methods Mol Biol 2022 ;2345:121-134

Cochrane Australia, Monash University, Melbourne, VIC, Australia.

Systematic reviews are difficult to keep up to date, but failure to do so leads to poor review currency and accuracy. "Living systematic review" (LSR) is an approach that aims to continually update a review, incorporating relevant new evidence as it becomes available. LSRs may be particularly important in fields where research evidence is emerging rapidly, current evidence is uncertain, and new research may change policy or practice decisions. Read More

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January 2022

Dysfunction of epithelial permeability barrier induced by HMGB1 in 2.5D cultures of human epithelial cells.

Tissue Barriers 2021 Sep 18:1972760. Epub 2021 Sep 18.

Department of Cell Science, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.

Airway and intestinal epithelial permeability barriers are crucial in epithelial homeostasis. High mobility group box 1 (HMGB1), increased by various stimuli, is involved in the induction of airway inflammation, as well as the pathogenesis of inflammatory bowel disease. HMGB1 enhances epithelial hyperpermeability. Read More

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September 2021

Myocardial deformation analysis in late-onset small-for-gestational-age and growth-restricted fetuses using two-dimensional speckle tracking echocardiography: a prospective cohort study.

J Perinat Med 2021 Sep 17. Epub 2021 Sep 17.

Department of Obstetrics and Gynecology, Technical University of Munich, School of Medicine, University Hospital rechts der Isar, Munich, Germany.

Objectives: An association between fetal growth restriction (FGR) and increased predisposition to cardiovascular disease (CVD) is suggested. The aim of this study was to evaluate subclinical signs of fetal cardiac remodeling in late-onset small-for-gestational-age (SGA) and growth-restricted fetuses using two-dimensional speckle tracking echocardiography (2D-STE).

Methods: This is a prospective cohort study, including 117 late-onset (≥32 weeks) SGA (birthweight≤10th centile) fetuses and 102 gestational age matched controls. Read More

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September 2021

Methodological challenges for living systematic reviews conducted during the COVID-19 pandemic: A concept paper.

J Clin Epidemiol 2021 Sep 12;141:82-89. Epub 2021 Sep 12.

Department I of Internal Medicine, Evidence-based Oncology Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany.

Background: A living systematic review (LSR) is an emerging review type that makes use of continual updating. In the COVID-19 pandemic, we were confronted with a shifting epidemiological landscape, clinical uncertainties and evolving evidence. These unexpected challenges compelled us to amend standard LSR methodology. Read More

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September 2021

The novel prognostic value of postoperative follow-up lateral spread response after microvascular decompression for hemifacial spasm.

J Neurosurg 2021 Sep 3:1-5. Epub 2021 Sep 3.

Departments of1Neurosurgery.

Objective: The lateral spread response (LSR) is an aberrant electrophysiological response in which a stimulus on one branch of the facial nerve spills over to other branches of the nerve, which can be captured by electrodes near each branch. The authors performed this study to evaluate the prognostic value of the follow-up LSR with a sufficient time interval from intraoperative LSR (IO-LSR) after microvascular decompression (MVD) for hemifacial spasm (HFS), excluding the interference of various intraoperative situations.

Methods: A total of 247 patients treated with MVD for HFS between June 2011 and March 2019 were enrolled in this study. Read More

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September 2021

A Randomized Double-Blind Parallel-Group Study to Evaluate the Long-Term Effects of a Medical Device Containing 0.3% Octatrienoic Acid in the Treatment of Grade III Actinic Keratosis.

Dermatol Ther (Heidelb) 2021 Oct 2;11(5):1751-1762. Epub 2021 Sep 2.

Dermatology Unit, University of Campania Luigi Vanvitelli, Naples, Italy.

Introduction: Actinic keratosis (AK) consists of skin lesions with a milder degree of keratinocytic atypia. It can be also referred to as "field of cancerization," which can potentially evolve to cutaneous squamous cell carcinoma (SCC). Several therapeutic options are currently available, but not all are indicated on hyperkeratotic lesions. Read More

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October 2021

Association between radicular low back pain and constipation: a retrospective cohort study using a real-world national database.

Pain Rep 2021 Sep-Oct;6(3):e954. Epub 2021 Aug 26.

Connor Integrative Health Network, University Hospitals Cleveland Medical Center, Cleveland, OH, USA.

Introduction: Previous observational studies have reported an association between lumbosacral radiculopathy (LSR), a form of low back pain (LBP) with nerve root involvement, and constipation. However, it is unclear whether this association is due to confounding variables such as comorbidities and medications.

Objectives: This study explores the possible association between LSR and constipation, with the hypothesis that adults with LSR have increased odds of developing constipation compared with those with nonradicular LBP. Read More

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Whole blood viscosity in patients with aortic stenosis.

Blood Coagul Fibrinolysis 2021 Oct;32(7):491-495

University of Health Sciences, Department of Cardiology, Dr Abdurrahman Yurtaslan Ankara Onkoloji Education and Research Hospital, Ankara, Turkey.

Whole blood viscosity (WBV) may promote endothelial shear stress, inflammation, and can accelerate the atherosclerotic process. We aimed to evaluate the relationship between WBV and aortic stenosis. The study included 209 participants of whom 49 patients had severe aortic stenosis, 98 patients had mild-to-moderate aortic stenosis and 62 patients served as control. Read More

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October 2021

Correlation of liver enhancement in gadoxetic acid-enhanced MRI with liver functions: a multicenter-multivendor analysis of hepatocellular carcinoma patients from SORAMIC trial.

Eur Radiol 2021 Aug 31. Epub 2021 Aug 31.

Department of Radiology, University Hospital, Ludwig Maximilian University of Munich, Marchioninistrasse 15, 81377, Munich, Germany.

Objectives: To evaluate the correlation between liver enhancement on hepatobiliary phase and liver function parameters in a multicenter, multivendor study.

Methods: A total of 359 patients who underwent gadoxetic acid-enhanced MRI using a standardized protocol with various scanners within a prospective multicenter phase II trial (SORAMIC) were evaluated. The correlation between liver enhancement on hepatobiliary phase normalized to the spleen (liver-to-spleen ratio, LSR) and biochemical laboratory parameters, clinical findings related to liver functions, liver function grading systems (Child-Pugh and Albumin-Bilirubin [ALBI]), and scanner characteristics were analyzed using uni- and multivariate analyses. Read More

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Lateral spread response of different facial muscles during microvascular decompression in hemifacial spasm.

Clin Neurophysiol 2021 10 12;132(10):2503-2509. Epub 2021 Aug 12.

Department of Neurosurgery, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Republic of Korea. Electronic address:

Objective: Interpreting lateral spread response (LSR) during microvascular decompression (MVD) for hemifacial spasm (HFS) is difficult when LSRs observed in different muscles do not match. We aimed to analyze LSR patterns recorded in both the orbicularis oris (oris) and mentalis muscles and their relationships with clinical outcomes.

Methods: The data of 1288 HFS patients who underwent MVD between 2015 and 2018 were retrospectively reviewed. Read More

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October 2021

Effects of HMGB1 on Tricellular Tight Junctions via TGF-β Signaling in Human Nasal Epithelial Cells.

Int J Mol Sci 2021 Aug 4;22(16). Epub 2021 Aug 4.

Department of Cell Science, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.

The airway epithelium of the human nasal mucosa acts as a physical barrier that protects against inhaled substances and pathogens via bicellular and tricellular tight junctions (bTJs and tTJs) including claudins, angulin-1/LSR and tricellulin. High mobility group box-1 (HMGB1) increased by TGF-β1 is involved in the induction of nasal inflammation and injury in patients with allergic rhinitis, chronic rhinosinusitis, and eosinophilic chronic rhinosinusitis. However, the detailed mechanisms by which this occurs remain unknown. Read More

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Correction to: CRISPR/Cas13a targeting of RNA virus in plants.

Authors:
Kul Bhushan

Plant Cell Rep 2021 Oct;40(10):2013

Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.

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October 2021

Values of Apparent Diffusion Coefficient and Lesion-to-Spinal Cord Signal Intensity in Diagnosing Solitary Pulmonary Lesions: Turbo Spin-Echo versus Echo-Planar Imaging Diffusion-Weighted Imaging.

Biomed Res Int 2021 10;2021:3345953. Epub 2021 Aug 10.

Department of Radiology, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou, China 510120.

Objective: This study is aimed at comparing the image quality and diagnostic performance of mean apparent diffusion coefficient (ADC) and lesion-to-spinal cord signal intensity ratio (LSR) derived from turbo spin-echo diffusion-weighted imaging (TSE-DWI) and echo-planar imaging- (EPI-) DWI in patients with a solitary pulmonary lesion (SPL).

Methods: 33 patients with SPL underwent chest imaging using EPI-DWI and TSE-DWI with = 600 s/mm in free breathing. A comparison of the distortion ratio (DR), signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) was drawn between the two techniques using a Wilcoxon signed-rank test. Read More

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October 2021

Endoscopic Microvascular Decompression for Hemifacial Spasm: A Technical Case Report Demonstrating the Benefits of the Angled Endoscope and Intraoperative Neuromonitoring.

Cureus 2021 Jul 23;13(7):e16586. Epub 2021 Jul 23.

Neurosurgery, Hospital of the University of Pennsylvania, Philadelphia, USA.

A 57-year-old female with eight years of hemifacial spasm (HFS) underwent endoscopic microvascular decompression (MVD) of the facial nerve. Baseline stimulation of the zygomatic branch of the facial nerve activated at 1.2 mA. Read More

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Quality-Controlled Sputum Analysis by Flow Cytometry.

J Vis Exp 2021 08 9(174). Epub 2021 Aug 9.

bioAffinity Technologies;

Sputum, widely used to study the cellular content and other microenvironmental features to understand the health of the lung, is traditionally analyzed using cytology-based methodologies. Its utility is limited because reading the slides is time-consuming and requires highly specialized personnel. Moreover, extensive debris and the presence of too many squamous epithelial cells (SECs), or cheek cells, often renders a sample inadequate for diagnosis. Read More

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Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition.

FASEB J 2021 09;35(9):e21742

Department of Cell Science, Research Institute for Frontier Medicine, Sapporo Medical University, Sapporo, Japan.

Withdrawal from contact inhibition is necessary for epithelial cancer precursor cells to initiate cell growth and motility. Nevertheless, little is understood about the mechanism for the sudden initiation of cell growth under static conditions. We focused on cellular junctions as one region where breaking out of contact inhibition occurs. Read More

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September 2021