10,966 results match your criteria lps-stimulated raw2647


Cell-Free Lactobacillus casei 21L10 Modulates Nitric Oxide Release and Cell Proliferation/Cell Death in Lipopolysaccharide-Challenged HT-29 Cells.

Inflammation 2021 Jul 29. Epub 2021 Jul 29.

Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Kosice, Slovakia.

Lactobacillus casei (L. casei) is one of the probiotic strains that may influence intestinal injury and inflammation in nonspecific intestinal diseases. We aimed to evaluate the effect of cell-free Lactobacillus casei 21L10 supernatant (LC) on the cell line HT-29 challenged with lipopolysaccharide (LPS) in order to modulate production of NO, cell proliferation, and apoptosis. Read More

View Article and Full-Text PDF

Antifracture, Antibacterial, and Anti-inflammatory Hydrogels Consisting of Silver-Embedded Curdlan Nanofibrils.

ACS Appl Mater Interfaces 2021 Jul 29. Epub 2021 Jul 29.

Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, People's Republic of China.

The bacterial exopolysaccharide Curdlan has a unique collagen-like triple helical structure and immune-modulation activities. Although there have been several types of Curdlan gels reported for antibacterial or wound healing purposes, none of them exhibit favorable mechanical properties for clinically applicable wound healing materials. Herein, we present a two-step approach for preparing Ag-embedded Curdlan hydrogels that are highly soft but are very stretchable compared with common polysaccharide-based hydrogels. Read More

View Article and Full-Text PDF

Chemical Constituents from a Mangrove-Derived Actinobacteria Isoptericola chiayiensis BCRC 16888 and Evaluation of Their Anti-NO Activity.

Chem Biodivers 2021 Jul 29. Epub 2021 Jul 29.

Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.

Cultivation of the actinobacteria strain Isoptericola chiayiensis, a mangrove-derived actinobacteria that was isolated from a mangrove soil collected in Chiayi County, resulted in the isolation of one new 2-furanone derivative, isopterfuranone (1), one new sesquiterpenoid, isopterchiayione (2), one new benzenoid derivative, isopterinoid (3), five new flavonoids, chiayiflavans A-E (4-8), and 4 metabolites isolated for the first time from nature source, methyl 3-(4-methyl-2,5-dioxopyrrolidin-3-yl)propanoate (9), 3-ethyl-4-methylpyrrolidine-2,5-dione (10), chiayiensol (11) and chiayiensic acid (12). Their structures were determined through in-depth spectroscopic and mass-spectrometric analyses. Most of the isolates showed potent inhibitory effects on NO production in LPS-stimulated RAW 264. Read More

View Article and Full-Text PDF

Exosomal miR-193b-5p as a regulator of LPS-induced inflammation in dairy cow mammary epithelial cells.

In Vitro Cell Dev Biol Anim 2021 Jul 26. Epub 2021 Jul 26.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing, 163319, People's Republic of China.

Exosomes are a type of extracellular vesicle that act as shuttles, transporting certain genetic information to other cells. MiRNA cargo within exosomes can regulate gene expression at the transcriptional level. The objective of this study was to investigate the exosomal miRNAs that regulate lipopolysaccharide (LPS)-induced inflammation in dairy cow mammary alveolar (Mac-T) cells. Read More

View Article and Full-Text PDF

The natural compound puerarin alleviates inflammation and apoptosis in experimental cell and rat preeclampsia models.

Int Immunopharmacol 2021 Jul 24;99:108001. Epub 2021 Jul 24.

Department of Obstetrics and Gynecology, the First Affiliated Hospital of University of Science and Technology of China Anhui Provincial Hospital, Hefei City, Anhui Province 230001, PR China. Electronic address:

Puerarin (Pue), an isoflavone derived from Radix puerariae, exerts anti-apoptosis and anti-inflammatory effects. However, the protective effect of Pue on PE is still unknown. The present study aimed to investigate whether Pue alleviates symptoms of PE and suppresses inflammation and apoptosis in vitro and in vivo. Read More

View Article and Full-Text PDF

Bioassay-guided study of the anti-inflammatory effect of Anoectochilus burmannicus ethanolic extract in RAW 264.7 cells.

J Ethnopharmacol 2021 Jul 23:114452. Epub 2021 Jul 23.

Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Muang Chiang Mai, Chiang Mai, 50200, Thailand. Electronic address:

Ethnopharmacological Relevance: Anoectochilus species is a small terrestrial orchid found in tropical and subtropical rain forest. These orchids are traditionally used extensively in China, Taiwan, and Vietnam due to their medicinal properties and therapeutic benefits. They are employed for treatment in different systems, such as stomach disorders, chest pain, arthritis, tumor, piles, boils, menstrual disorders, and inflammation. Read More

View Article and Full-Text PDF

Meloxicam Inhibited the Proliferation of LPS-Stimulated Bovine Endometrial Epithelial Cells Through Wnt/β-Catenin and PI3K/AKT Pathways.

Front Vet Sci 2021 9;8:637707. Epub 2021 Jul 9.

College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.

Meloxicam is a non-steroidal anti-inflammatory drug and has been used to relieve pain and control inflammation in cows with metritis and endometritis. Meloxicam has been found to be effective in inhibiting tissue or cell growth when it is used as an anti-inflammatory therapy. However, the influence of meloxicam on bovine endometrial regeneration has not been reported. Read More

View Article and Full-Text PDF

Butorphanol Promotes Macrophage Phenotypic Transition to Inhibit Inflammatory Lung Injury κ Receptors.

Front Immunol 2021 7;12:692286. Epub 2021 Jul 7.

Department of Anesthesiology, Affiliated Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is characterized by diffuse inflammation of the lung parenchyma and refractory hypoxemia. Butorphanol is commonly used clinically for perioperative pain relief, but whether butorphanol can regulate LPS-induced alveolar macrophage polarization is unclear. In this study, we observed that butorphanol markedly attenuated sepsis-induced lung tissue injury and mortality in mice. Read More

View Article and Full-Text PDF

Diterpenoids Isolated from Inhibited the Inflammatory Mediators in LPS-Induced HT-29 and RAW 264.7 Cells.

Molecules 2021 Jul 17;26(14). Epub 2021 Jul 17.

College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon-si 57922, Jeonnam, Korea.

Species of are used traditionally in their native areas for the treatment of fevers, asthma, coughs, cholera, chest pain, arthritis, rheumatism, and sexually transmitted diseases. To identify natural products having efficacy against inflammatory bowel disease (IBD), we identified a new, 16-hydroxy-4β-carboxy--β-D-glucopyranosyl-19-nor-totarol () together with three known diterpenoids from . Furthermore, all the extracts, fractions, and isolates - were investigated for their anti-inflammatory effects by assessing the expression on nitric oxide (NO) production and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264. Read More

View Article and Full-Text PDF

Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells.

Int J Mol Sci 2021 Jul 13;22(14). Epub 2021 Jul 13.

College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Korea.

Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of L. The dried flowers of were extracted with 70% EtOH, and the obtained extract was divided into CHCl, EtOAc, -BuOH, and HO fractions. Read More

View Article and Full-Text PDF

Sulfatides are endogenous ligands for the TLR4-MD-2 complex.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

Center for the Genetics of Host Defense, University of Texas Southwestern Medical Center, Dallas, TX 75390;

Many endogenous molecules, mostly proteins, purportedly activate the Toll-like receptor 4 (TLR4)-myeloid differentiation factor-2 (MD-2) complex, the innate immune receptor for lipopolysaccharide (LPS) derived from gram-negative bacteria. However, there is no structural evidence supporting direct TLR4-MD-2 activation by endogenous ligands. Sulfatides (3--sulfogalactosylceramides) are natural, abundant sulfated glycolipids that have variously been shown to initiate or suppress inflammatory responses. Read More

View Article and Full-Text PDF

Gallic acid mitigates LPS-induced inflammatory response via suppressing NF-κB signalling pathway in IPEC-J2 cells.

J Anim Physiol Anim Nutr (Berl) 2021 Jul 20. Epub 2021 Jul 20.

Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China.

Gallic acid is a phenolic compound that exhibits antibacterial, antioxidative and anti-inflammatory functions. In a previous study, we found that dietary supplementation with gallic acid decreased incidence of diarrhoea and protected intestinal integrity in weaning piglets. However, the underlying mechanism remains unclear. Read More

View Article and Full-Text PDF

Aloe-emodin prevents nerve injury and neuroinflammation caused by ischemic stroke the PI3K/AKT/mTOR and NF-κB pathway.

Food Funct 2021 Jul 20. Epub 2021 Jul 20.

Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangzhou 510006, China. and Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangzhou 510006, China and School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.

Ischemic stroke (IS) caused by cerebral arterial occlusion is the leading cause of global morbidity and mortality. Cellular oxidative stress and inflammation play a vital role in the pathological process of neural damage in IS. It is necessary to develop functional food or drugs, which target neuroinflammation and oxidation mechanisms against IS. Read More

View Article and Full-Text PDF

Isoginkgetin treatment attenuated lipopolysaccharide-induced monoamine neurotransmitter deficiency and depression-like behaviors through downregulating p38/NF-κB signaling pathway and suppressing microglia-induced apoptosis.

J Psychopharmacol 2021 Jul 19:2698811211032473. Epub 2021 Jul 19.

Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong, China.

Background: Microglia activation-induced neuroinflammation may contribute to the etiology of depression. containing high concentration of isoginkgetin could effectively treat mental diseases in ancient times. However, the therapeutic role, peculiarly in the brain-immune modulation in depression is still unclear. Read More

View Article and Full-Text PDF

A novel indazole derivative, compound Cyy-272, attenuates LPS-induced acute lung injury by inhibiting JNK phosphorylation.

Toxicol Appl Pharmacol 2021 Jul 16:115648. Epub 2021 Jul 16.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China; Institute of Chronic Kidney Disease, Wenzhou Medical University, Wenzhou, China. Electronic address:

Acute lung injury (ALI) is a diffuse lung dysfunction disease characterized by high prevalence and high mortality. Thus far, no effective pharmacological treatment has been made for ALI in clinics. Inflammation is critical to the development of ALI. Read More

View Article and Full-Text PDF

Anti-inflammatory effect of Ailanthus altissima (Mill.) Swingle leaves in lipopolysaccharide-stimulated astrocytes.

J Ethnopharmacol 2021 Jul 13:114258. Epub 2021 Jul 13.

Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Activated astrocytes are involved in the progression of neurodegenerative diseases. Traditionally, Ailanthus altissima (Mill.) Swingle, widely distributed in East Asia, has been used as a medicine for the treatment of fever, gastric diseases, and inflammation. Read More

View Article and Full-Text PDF

Inflammation protein quantification by MRM mass spectrometry in LPS stimulated THP-1 cells.

Rapid Commun Mass Spectrom 2021 Jul 16:e9166. Epub 2021 Jul 16.

Department of Chemical Sciences, University of Naples Federico II, Naples, Italy.

Rationale: Inflammation is a cascade of events mediated by a cytokine network triggering the cellular response. In order to monitor the modulation of the crucial inflammatory proteins, e.g. Read More

View Article and Full-Text PDF

Nargenicin A1 attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB signaling pathway.

EXCLI J 2021 28;20:968-982. Epub 2021 May 28.

Anti-Aging Research Center, Dong-eui University, Busan, Republic of Korea.

Inflammation caused by the excessive production of pro-inflammatory mediators and cytokines in abnormally activated macrophages promotes the initiation and progression of many diseases along with oxidative stress. Previous studies have suggested that nargenicin A1, an antibacterial macrolide isolated from sp. may be a potential treatment for inflammatory responses and oxidative stress, but the detailed mechanisms are still not well studied. Read More

View Article and Full-Text PDF

Sulfated extract of Abelmoschus esculentus: A potential cancer chemopreventive agent.

Curr Pharm Biotechnol 2021 Jul 14. Epub 2021 Jul 14.

Radiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

Background: Abelmoschus esculentus (AE) (okra), is an edible plant used in many food applications.

Objective: This study explored whether sulfated AE (SAE) has promising cancer chemopreventive activities that may recommend it as a functional food supplement ‎instead of (or in addition to) AE for the population at risk of cancer and in the health food industry.

Methods: Cytochrome P450-1A (CYP1A)‎ was estimated by fluorescence enzymatic reaction, using β-naphthoflavone-treated cells (CYP1A inducer). Read More

View Article and Full-Text PDF

Effects of Eugenol and Dehydrodieugenol B from against Lipopolysaccharide (LPS)-Induced Experimental Acute Lung Inflammation.

J Nat Prod 2021 Jul 15. Epub 2021 Jul 15.

Department of Biological Science, Federal University of São Paulo, Diadema, SP, 09913-030, Brazil.

Acute lung injury (ALI) is an important public health problem. The present work investigated whether dehydrodieugenol B treatment, a compound isolated from Brazilian plant (Lauraceae), modulates experimental ALI and compared the observed effects to eugenol. Effects of dehydrodieugenol B in lipopolysaccharide (LPS)-stimulated RAW 264. Read More

View Article and Full-Text PDF

Fisetin Ameliorates the Inflammation and Oxidative Stress in Lipopolysaccharide-Induced Endometritis.

J Inflamm Res 2021 5;14:2963-2978. Epub 2021 Jul 5.

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, People's Republic of China.

Purpose: Fisetin is a natural flavone of polyphenol, which widely exists in many fruits and vegetables and has many pharmacological activities. However, the mechanism involved remains largely unknown. Here, we investigate the mechanisms of fisetin on the inflammatory response and oxidative stress in LPS-induced endometritis model and bovine endometrial epithelial cell line (BEND). Read More

View Article and Full-Text PDF

In-vitro, in-vivo, and in-silico assessment of radical scavenging and cytotoxic activities of Oliveria decumbens essential oil and its main components.

Sci Rep 2021 Jul 12;11(1):14281. Epub 2021 Jul 12.

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

We aimed to explore and compare new insights on the pharmacological potential of Oliveria decumbence essential oil (OEO) and its main components highlighting their antioxidant activity in-vitro, in-vivo, and in-silico and also cytotoxic effects of OEO against A549 lung cancer cells. At first, based on GC-MS analysis, thymol, carvacrol, p-cymene, and γ-terpinene were introduced as basic ingredients of OEO and their in-vitro antioxidant capacity was considered by standard methods. Collectively, OEO exhibited strong antioxidant properties even more than its components. Read More

View Article and Full-Text PDF

Naringenin inhibits pro‑inflammatory cytokine production in macrophages through inducing MT1G to suppress the activation of NF‑κB.

Mol Immunol 2021 Jul 9;137:155-162. Epub 2021 Jul 9.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China. Electronic address:

Naringenin (Nar) is a flavanone that has been suggested to provide human health benefits such as anti-inflammatory, anti-oxidant and anti-cancer properties. However, the mechanisms underlying these benefits are complex and still not fully understood. In this study, we investigated the effect of Nar on the inflammatory response of macrophages and its underlying mechanism. Read More

View Article and Full-Text PDF

New oligomeric neolignans from the leaves of Magnolia officinalis var. biloba.

Chin J Nat Med 2021 Jul;19(7):491-499

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Six new oligomeric neolignans including two trimeric neolignans (1 and 2) and four dimeric neolignans (3-6) were isolated from the leaves of Magnolia officinalis var. biloba. Their structures were determined based on HR-ESIMS and NMR data, as well as electronic circular dichroism (ECD) calculations. Read More

View Article and Full-Text PDF

TAK1 in the AP-1 pathway is a critical target of Saururus chinensis (Lour.) Baill in its anti-inflammatory action.

J Ethnopharmacol 2021 Jul 7;279:114400. Epub 2021 Jul 7.

Department of Integrative Biotechnology, Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Saururus chinensis (Lour.) Baill (Saururaceae), also known as Asian lizard's tail, is a plant commonly found in East Asia. Its leaves have been used in traditional medicine to treat many diseases such as edema, pneumonia, hypertension, leproma, jaundice, gonorrhea, and rheumatoid arthritis. Read More

View Article and Full-Text PDF

Hydrogen alleviates cell damage and acute lung injury in sepsis via PINK1/Parkin-mediated mitophagy.

Inflamm Res 2021 Aug 10;70(8):915-930. Epub 2021 Jul 10.

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin Research Institute of Anesthesiology, 154 Anshan Road, Tianjin, 300052, People's Republic of China.

Background: Multiple organ failure (MOF) is the main cause of early death in septic shock. Lungs are among the organs that are affected in MOF, resulting in acute lung injury. Inflammation is an important factor that causes immune cell dysfunction in the pathogenesis of sepsis. Read More

View Article and Full-Text PDF

HIPK2 phosphorylates HDAC3 for NF-κB acetylation to ameliorate colitis-associated colorectal carcinoma and sepsis.

Proc Natl Acad Sci U S A 2021 Jul;118(28)

Institute of Geriatrics, Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Shanghai 200444, China;

Although inflammation is critical for the clearance of pathogens, uncontrolled inflammation also contributes to the development of multiple diseases such as cancer and sepsis. Since NF-κB-mediated transactivation in the nucleus is pivotal downstream of various stimuli to induce inflammation, searching the nuclear-localized targets specifically regulating NF-κB activation will provide important therapeutic application. Here, we have identified that homeodomain-interacting protein kinase 2 (HIPK2), a nuclear serine/threonine kinase, increases its expression in inflammatory macrophages. Read More

View Article and Full-Text PDF

lncRNA NEAT1 aggravates sepsis-induced lung injury by regulating the miR-27a/PTEN axis.

Lab Invest 2021 Jul 8. Epub 2021 Jul 8.

NHC Key Laboratory of Pulmonary Immune-related Diseases, Guizhou Provincial People's Hospital GZU, Guiyang, P. R. China.

Sepsis is an acute inflammatory reaction and a cause of acute respiratory distress syndrome (ARDS). In the present study, we explored the roles and underlying mechanism of the lncRNA Nuclear enriched abundant transcript 1 (NEAT1) in ARDS. The expression levels of genes, proteins and pro-inflammatory cytokines in patients with ARDS, LPS-stimulated cells and septic mouse models were quantified using qPCR, western blotting and ELISA assays, respectively. Read More

View Article and Full-Text PDF

Antibacterial and anti-inflammatory activity of valproic acid-pyrazole conjugates as a potential agent against periodontitis.

Drug Dev Res 2021 Jul 6. Epub 2021 Jul 6.

School of Stomatology, Anhui Medical College, Heifei, Anhui, China.

Periodontitis is a serious global concern. Therefore, in the present study, we intend to synthesize novel valproic-acid pyrazole conjugates as a novel agent against periodontitis. The molecules were developed in a facile synthetic route and obtained in excellent yields. Read More

View Article and Full-Text PDF

Revisiting bupropion anti-inflammatory action: involvement of the TLR2/TLR4 and JAK2/STAT3.

Inflammopharmacology 2021 Aug 3;29(4):1101-1109. Epub 2021 Jul 3.

Department of Pharmacology, School of Pharmacy, Shahid Sadoughi University of Medical Sciences and Health Services, Shohaday-e-Gomnam Blvd., Alem Sq., Yazd, Iran.

There are accumulating reports regarding poor response to common antidepressant therapy. Antidepressant resistance is often linked to inflammatory system activation and patients displaying inflammation prior to the treatment are less responsive to antidepressants. We hypothesized that the inefficacy of antidepressant therapy in some patients may be attributable to the drugs' inflammatory mode of action, which has been overlooked because of their substantial therapeutic benefit. Read More

View Article and Full-Text PDF