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Validation of post-harvest antimicrobial interventions to control Shiga toxin-producing Escherichia coli (STEC) on market hog carcass surfaces.

Int J Food Microbiol 2021 Nov 24;358:109421. Epub 2021 Sep 24.

Animal Sciences and Industry Department, Kansas State University, Manhattan, KS 66506, USA. Electronic address:

Although swine are less associated with STEC foodborne disease outbreaks, the potential for swine to serve as a source of STEC infections in human beings cannot be disregarded. This study compared eight USDA-approved antimicrobial intervention technologies to quantify their ability to reduce STEC contamination on market hog carcasses. Hogs were harvested to provide skin-on carcass sides, and eight sides (per three replications) were inoculated with a 7-strain STEC cocktail (ca. Read More

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November 2021

The EyeFlowCell: Development of a 3D-Printed Dissolution Test Setup for Intravitreal Dosage Forms.

Pharmaceutics 2021 Sep 3;13(9). Epub 2021 Sep 3.

Center of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, Germany.

An in vitro dissolution model, the so-called EyeFlowCell (EFC), was developed to test intravitreal dosage forms, simulating parameters such as the gel-like consistency of the vitreous body. The developed model consists of a stereolithography 3D-printed flow-through cell with a polyacrylamide (PAA) gel as its core. This gel needed to be coated with an agarose sheath because of its low viscosity. Read More

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September 2021

Degradation of steroid estrogens by UV/peracetic acid: Influencing factors, free radical contribution and toxicity analysis.

Chemosphere 2021 Sep 15;287(Pt 3):132261. Epub 2021 Sep 15.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China. Electronic address:

Steroid estrogens (SEs) are a group of refractory organic micropollutants detected in secondary effluent frequently. The advanced oxidation processes (AOPs) are usually used to deep remove the SEs from the secondary effluent. Herein, we first investigated the UV/peracetic acid (PAA), a PAA-based AOP, to degrade SEs. Read More

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September 2021

Development of highly sensitive and selective bisphenol A sensor based on a cobalt phthalocyanine-modified carbon paste electrode: application in dairy analysis.

Anal Methods 2021 Oct 14;13(39):4674-4682. Epub 2021 Oct 14.

Kathleen Lonsdale Institute for Human Health Research, Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland.

The development of an accurate, sensitive and selective sensor for the detection of bisphenol A (BPA) based on the incorporation of a new phthalocyanine derivative, cobalt phthalocyanine, C,C,C,C-tetracarboxylic acid-polyacrylamide (CoPc-PAA) into a carbon-paste matrix is presented using voltammetry and constant potential techniques. The influence of measuring parameters such as pH and scan rate on the analytical performance of the sensor was evaluated. Several kinetic parameters such as electron transfer number (), charge transfer coefficient (), electrode surface area () and diffusion coefficient () were also calculated. Read More

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October 2021

Dry-fog decontamination of microbiological safety cabinets after activities with SARS-CoV-2: cycle development and process validation for dry fogging with peroxyacetic acid.

GMS Hyg Infect Control 2021 31;16:Doc26. Epub 2021 Aug 31.

Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.

Technical protection measures for laboratory activities involving biological agents include biological safety cabinets (BSC) that may be contaminated. In the case of diagnostic activities with SARS-CoV-2, this may also affect BSC that are operated at protection level 2; therefore, decontamination of all contaminated surfaces of the BSC may be required. In addition to fumigation with hydrogen peroxide (HO), dry fogging of HO-stabilized peroxyacetic acid (PAA) represents another alternative to fumigation with formalin. Read More

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The biosynthesis of ditropolonyl sulfide, an antibacterial compound produced by complex strain R-12632.

Appl Environ Microbiol 2021 Sep 15:AEM0116921. Epub 2021 Sep 15.

Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.

complex strain R-12632 produces ditropolonyl sulfide, an unusual sulfur-containing tropone, via a yet unknown biosynthetic pathway. Ditropolonyl sulfide purified from a culture of strain R-12632 inhibits the growth of various Gram-positive and Gram-negative multidrug resistant bacteria, with minimum inhibitory concentration (MIC) values as low as 16 μg/ml. In the present study we used a transposon mutagenesis approach combined with metabolite analyses to identify the genetic basis for antibacterial activity of strain R-12632 against Gram-negative bacterial pathogens. Read More

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September 2021

Oral delivery of proteins and peptides: Challenges, status quo and future perspectives.

Acta Pharm Sin B 2021 Aug 29;11(8):2416-2448. Epub 2021 Apr 29.

Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China.

Proteins and peptides (PPs) have gradually become more attractive therapeutic molecules than small molecular drugs due to their high selectivity and efficacy, but fewer side effects. Owing to the poor stability and limited permeability through gastrointestinal (GI) tract and epithelia, the therapeutic PPs are usually administered by parenteral route. Given the big demand for oral administration in clinical use, a variety of researches focused on developing new technologies to overcome GI barriers of PPs, such as enteric coating, enzyme inhibitors, permeation enhancers, nanoparticles, as well as intestinal microdevices. Read More

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In Vitro Antibiotic Resistance among Bacteria from the Cornea in the Antibiotic Resistance Monitoring in Ocular MicRoorganisms Surveillance Study.

Optom Vis Sci 2021 09;98(9):1113-1121

University of Tennessee Health Science Center, Knoxville, Tennessee.

Purpose: This study aimed to report on in vitro susceptibility patterns among corneal isolates collected in the Antibiotic Resistance Monitoring in Ocular micRoorganisms (ARMOR) study.

Methods: Each year, from 2009 to 2019, Staphylococcus aureus, coagulase-negative staphylococci (CoNS), Streptococcus pneumoniae, Pseudomonas aeruginosa, and Haemophilus influenzae isolates cultured from patients with ocular infections at participating ARMOR sites were submitted to a central laboratory for species confirmation and antibiotic susceptibility testing. In this analysis of corneal isolates, odds ratios for concurrent resistance were based on sample proportions, one-way ANOVA was used to evaluate resistance by patient age, and Cochran-Armitage tests were used to examine changes in antibiotic resistance over time. Read More

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September 2021

[Preparation of multi-functional magnetic nanoparticles for harvesting low-molecular-weight glycoproteins].

Se Pu 2021 Oct;39(10):1102-1110

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Low-molecular-weight glycoproteins (LMW-GPs) are considered promising candidates for disease biomarker discovery. Selective sorbents are essential for the extraction and enrichment of this class of compounds. Boronate affinity chromatography is a unique separation mode in liquid chromatography. Read More

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October 2021

Water-Preserving and Salt-Resistant Slow-Release Fertilizers of Polyacrylic Acid-Potassium Humate Coated Ammonium Dihydrogen Phosphate.

Polymers (Basel) 2021 Aug 24;13(17). Epub 2021 Aug 24.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

Polyacrylic acid (PAA) has high water absorbency but poor salt resistance. Humic acid (HA) extracted from lignite was introduced into the cross-linked copolymer systems of AA to improve the water absorbency and salt-tolerance. A polyacrylic acid-potassium humate (PAA-KHA) coated ammonium dihydrogen phosphate (ADP) fertilizer with water-preserving, salt-resistant and slow-release properties was prepared. Read More

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Efficient Fenton-Like Catalysis Boosting the Antifouling Performance of the Heterostructured Membranes Fabricated via Vapor-Induced Phase Separation and In Situ Mineralization.

ACS Appl Mater Interfaces 2021 Sep 3;13(36):43648-43660. Epub 2021 Sep 3.

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.

A photocatalytic membrane with significant degradation and antifouling performance has become an important part in wastewater treatment. However, the low catalyst loading on the polymer membrane limits its performance improvement. Herein, we fabricated poly(vinylidene fluoride) (PVDF) and poly(acrylic acid) (PAA) blend membranes with a rough surface via a vapor-induced phase separation (VIPS) process. Read More

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September 2021

The Value of Brain Resting-State Functional Magnetic Resonance Imaging on Image Registration Algorithm in Analyzing Abnormal Changes of Neuronal Activity in Patients with Type 2 Diabetes.

Contrast Media Mol Imaging 2021 13;2021:6951755. Epub 2021 Aug 13.

Department of Medical Imaging, Hebei Provincial People's Hospital, Shijiazhuang, Hebei 050051, China.

The aim of this paper was to analyze the application value of resting-state functional magnetic resonance imaging (FMRI) parameters and rigid transformation algorithm in patients with type 2 diabetes (T2DM), which could provide a theoretical basis for the registration application of FMRI. 107 patients confirmed pathologically as T2DM and 51 community medical healthy volunteers were selected and divided into an experimental group and a control group, respectively. Besides, all the subjects were scanned with FMRI. Read More

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Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation.

Int J Nanomedicine 2021 21;16:5675-5692. Epub 2021 Aug 21.

General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, People's Republic of China.

Purpose: Since immune cells in the tumor microenvironment (TME) can affect the development and progression of tumors, strategies modulating immune cells are considered to have an important therapeutic effect. As a TLR7/8 agonist, R848 effectively activates the innate immune cells to exert an anti-tumor effect. Mn has been reported to strongly promote the maturation of antigen-presenting cells (APCs), thereby enhancing the cytotoxicity of CD8 T cells. Read More

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Fabrication and In Vitro Evaluation of pH-Sensitive Polymeric Hydrogels as Controlled Release Carriers.

Gels 2021 Aug 5;7(3). Epub 2021 Aug 5.

School of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung City 80708, Taiwan.

The purpose of the current investigation was to develop chondroitin sulfate/carbopol-co-poly(acrylic acid) (CS/CBP-co-PAA) hydrogels for controlled delivery of diclofenac sodium (DS). Different concentrations of polymers chondroitin sulfate (CS), carbopol 934 (CBP), and monomer acrylic acid (AA) were cross-linked by ethylene glycol dimethylacrylate (EGDMA) in the presence of ammonium peroxodisulfate (APS) (initiator). The fabricated hydrogels were characterized for further experiments. Read More

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Identification of micropollutants from graywater of different complexity and remediation using multilayered membranes.

Environ Sci Pollut Res Int 2021 Aug 17. Epub 2021 Aug 17.

School of Environmental Studies, Cochin University of Science and Technology, Kochi, Kerala, 682022, India.

Graywater reuse is one of the important concepts in attaining water sustainability. A major challenge in this area is to realize various components present in graywater. The present study involves the identification of the chemical components of graywater collected from three different environments and to investigate the efficiency of removal of some of these chemical components using ultrafiltration membranes (polyelectrolyte multilayer (PEM) membranes). Read More

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Improving the Stability of Non-Noble-Metal M-N-C Catalysts for Proton-Exchange-Membrane Fuel Cells through M-N Bond Length and Coordination Regulation.

Adv Mater 2021 Oct 15;33(39):e2006613. Epub 2021 Aug 15.

State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.

An effective and universal strategy is developed to enhance the stability of the non-noble-metal M-N /C catalyst in proton exchange membrane fuel cells (PEMFCs) by improving the bonding strength between metal ions and chelating polymers, i.e., poly(acrylic acid) (PAA) homopolymer and poly(acrylic acid-maleic acid) (P(AA-MA)) copolymer with different AA/MA ratios. Read More

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October 2021

Plasma amino acid levels in individuals with bacterial pneumonia and healthy controls.

Hideki Ikeda

Clin Nutr ESPEN 2021 08 2;44:204-210. Epub 2021 Jul 2.

Department of Pulmonary Medicine, Sanyudo Hospital, Yonezawa, Japan. Electronic address:

Background & Aims: Amino acids play an important role in immune responses and as neurotransmitters. During the course of a bacterial pneumonia episode, from the onset to the recovery phase, immune responses dramatically change, as does the metabolism of amino acids, a concept referred to as immuno-nutrition. We investigated the differences in plasma amino acid levels (PAA) between the acute and recovery phases in individuals with community-acquired pneumonia (CAP) and healthy controls. Read More

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Comparative analysis of peracetic acid (PAA) and permaleic acid (PMA) in disinfection processes.

Sci Total Environ 2021 Nov 21;797:149206. Epub 2021 Jul 21.

Department of Medicine Surgery and Dentistry, University of Salerno, via S. Allende, 84081 Baronissi, SA, Italy.

The growing demand to reduce chlorine usage and control disinfection byproducts increased the development of new strategies in wastewater treatments. Organic peracids are increasingly attracting interest in disinfection activities as a promising alternative to chlorine and chlorine-based agents. In this study, we assessed the antimicrobial properties against Escherichia coli (E. Read More

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November 2021

Bio-inspired dual-functional phospholipid-poly(acrylic acid) brushes grafted porous poly(vinyl alcohol) beads for selective adsorption of low-density lipoprotein.

J Mater Chem B 2021 Aug 23;9(32):6364-6376. Epub 2021 Jul 23.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Elevated levels of low-density lipoproteins (LDL) are recognized as a crucial indicator of hyperlipidemia (HLP) and lowering of LDL levels represents an effective clinical treatment strategy. Inspired by the conjugation of phospholipid monolayers and the lipid content of the LDL particle, the current study describes the preparation of an innovative hemoperfusion adsorbent. The adsorbent was prepared by attachment of phosphatidyl ethanolamine to poly(acrylic acid) modified poly(vinyl alcohol-co-triallyl isocyanurate) beads ([email protected]). Read More

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Mycotic Popliteal Artery Aneurysm With Rapid Enlargement Post-Bypass.

Cureus 2021 Jun 18;13(6):e15746. Epub 2021 Jun 18.

Surgery, Brooklyn Veterans Affairs Medical Center, Brooklyn, USA.

Popliteal artery aneurysms (PAAs) are the most common type of peripheral artery aneurysms. Mycotic aneurysms involving the popliteal artery are quite rare and can occur as either a primary infection or a secondary infection from another site. To our knowledge, there are no previous case reports on mycotic PAA in which was the primary etiologic pathogen. Read More

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Resection and reconstruction of pancreatic artery aneurysms caused by the compression of the celiac trunk by the median arcuate ligament: a report of two cases.

Surg Case Rep 2021 Jul 16;7(1):167. Epub 2021 Jul 16.

Department of Surgery, Saiseikai Fukuoka General Hospital, Fukuoka, 810-0001, Japan.

Background: Some patients with the compression of the celiac trunk by the median arcuate ligament (MAL) suffer pancreatic artery aneurysms (PAAs) due to excessive blood flow from the superior mesenteric artery. These aneurysms are in peril because they are prone to rupture irrespective of size. Here, we present two cases of resection and reconstruction of PAAs caused by the compression of the celiac trunk by the MAL. Read More

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Mechanistic modeling of peracetic acid wastewater disinfection using computational fluid dynamics: Integrating solids settling with microbial inactivation kinetics.

Water Res 2021 Aug 11;201:117355. Epub 2021 Jun 11.

US Peroxide Technologies, 1375 Peachtree St. NE, Suite 300 N., Atlanta, GA, USA. Electronic address:

While the impact of suspended solids on chemical disinfection kinetics has been widely recognized, a detailed modeling framework for assessing their contribution on disinfection efficiency in municipal contact tanks is yet unavailable. In this paper, we conducted experimental and modeling studies to mechanistically describe the interplay between suspended solids (not removed by gravity settling in secondary clarifiers) and disinfection performance of an emerging disinfectant, peracetic acid, operated in a municipal contact tank. Specifically, we developed an integrated computational fluid dynamics (CFD) model to simultaneously predict the fate and transport of suspended solids, Escherichia coli and peracetic acid in a hypothetical reactor using an exposure-based (i. Read More

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Layer by layer assembled functionalized graphene oxide-based polymer brushes for superlubricity on steel-steel tribocontact.

Soft Matter 2021 Jul;17(29):7014-7031

Environmental Engineering Division, CSIR - Central Mechanical Engineering Research Institute, Durgapur - 713209, India. and Academy of Scientific and Innovative Research (AcSIR), Ghaziabad - 201002, India.

This study demonstrates a simple and multistep approach for a covalent functionalization of chemically-prepared graphene oxide (GO) using branched polyethylenimine (PEI) through nucleophilic addition reaction to prepare GO-PEI. Further layer-by-layer (LBL) assembly on functionalized GO-PEI with anionic polyelectrolyte, poly(acrylic acid sodium salt) (PAA) and poly(sodium 4-styrenesulfonate) (PSS) have been undertaken to fabricate polymer brushes (PB). The physicochemical structures of GO, GO-PEI and LBL assembled PB [GO-PEI-PAA and GO-PEI-PSS] have been explored using standard spectral and morphological analysis. Read More

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Penile Doppler ultrasound study in priapism: A systematic review.

Prog Urol 2021 Jul 3. Epub 2021 Jul 3.

Médecine Translationnelle et Epidémiologie, Laboratoire des Sciences de la Santé, Faculté de Médecine et de Pharmacie, Université Ibn Zohr, 80000 Agadir, Morocco; Department of Pharmacology, University Hospital of Agadir, 80000 Agadir, Morocco.

Background: Penile Doppler ultrasound (PDU) is suggested to be an alternative to blood gas analysis (BGA) from the corpora cavernosa in differentiating between high- and low-flow priapisms, with limited supportive evidence.

Aim: To compare penile Doppler ultrasound study and blood gas analysis in the diagnosis of priapism, through a systematic review of the literature.

Methods: Studies were identified by literature search of Medline, Scopus, Cochrane and ClinicalTrials. Read More

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A pH-responsive mesoporous silica nanoparticles-based drug delivery system with controlled release of andrographolide for OA treatment.

Regen Biomater 2021 Aug 30;8(4):rbab020. Epub 2021 Jun 30.

Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, No. 22 Shuangyong Road, Qingxiu District, Nanning 530021, China.

Andrographolide (AG) has favorable anti-inflammatory and antioxidative capacity. However, it has low bioavailability due to high lipophilicity and can be easily cleared by the synovial fluid after intra-articular injection, leading to low therapeutic efficiency in osteoarthritis (OA). Herein, we designed a nano-sized pH-responsive drug delivery system (DDS) for OA treatment by using modified mesoporous silica nanoparticles (MSNs) with pH-responsive polyacrylic acid (PAA) for loading of AG to form [email protected] nanoplatform. Read More

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Effects of bovine cancellous bone powder/poly amino acid composites on cellular behaviors and osteogenic performances.

Biomed Mater 2021 07 6;16(5). Epub 2021 Jul 6.

School of Automation and Information Engineering, Sichuan University of Science and Engineering, Zigong, People's Republic of China.

Xenogeneic bone has good biological activity, but eliminating immunogenicity, while retaining osteogenic abilities, is a challenge. By combining xenogeneic bone with poly amino acid (PAA) that has an amide bond structure, a new type of composite conforming to bionics and low immunogenicity may be obtained. In this study, according to the principles of component bionics, three composites of delipidized cancellous bone powder (DCBP) and PAA were designed and obtained by anpolycondensation method, an extrusion molding (EM) method, and a solution-blend method. Read More

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Nafion-Based Proton-Exchange Membranes Built on Cross-Linked Semi-Interpenetrating Polymer Networks between Poly(acrylic acid) and Poly(vinyl alcohol).

ACS Appl Mater Interfaces 2021 Jun 14;13(24):28188-28200. Epub 2021 Jun 14.

Organic Material Synthesis Laboratory, Department of Chemistry, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.

We report semi-interpenetrating polymer network (semi-IPN) membranes prepared easily from a cross-linked network using poly(acrylic acid) (PAA) and poly(vinyl alcohol) (PVA) with interpenetrated Nafion for both proton-exchange membrane fuel cell (PEMFC) and proton-exchange membrane water electrolyzer (PEMWE) applications. Thermal esterification between PAA and PVA induced three-dimensional cross-linking to improve mechanical toughness and reduce hydrogen crossover, while the hydrophilic nature of the PAA-PVA-based cross-linked matrix still enhanced the water uptake (WU) and hence conductivity of the Nafion penetrant. The semi-IPN membrane (NPP-95) composed of Nafion, PAA, and PVA with a ratio of 95:2. Read More

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Exposures of Phenylacetic Acid and Phenylacetylglutamine Across Different Subpopulations and Correlation with Adverse Events.

Clin Pharmacokinet 2021 Jun 14. Epub 2021 Jun 14.

Clinical Development, Mallinckrodt Pharmaceuticals, Hampton, NJ, 08827, USA.

Background: Elevated plasma ammonia is central to the pathogenesis of hepatic encephalopathy. Sodium phenylacetate or glycerol phenylbutyrate is approved for urea cycle disorders, but limited clinical data are available for hepatic encephalopathy. Phenylacetic acid (PAA) plasma exposure has been reported to correlate with neurologic adverse events in patients with cancer but not in patients with urea cycle disorders or hepatic encephalopathy. Read More

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Easy Surface Functionalization and Bioconjugation of Peptides as Capture Agents of a Microfluidic Biosensing Platform for Multiplex Assay in Serum.

Bioconjug Chem 2021 08 11;32(8):1593-1601. Epub 2021 Jun 11.

Center for Advanced Biomaterials for [email protected], Istituto Italiano di Tecnologia (IIT), Largo Barsanti e Matteucci 53, 80125 Naples, Italy.

The development of assays for protein biomarkers in complex matrices is a demanding task that still needs implementation of new approaches. Antibodies as capture agents have been largely used in bioassays but their low stability, low-efficiency production, and cross-reactivity in multiplex approaches impairs their larger applications. Instead, synthetic peptides, even with higher stability and easily adapted amino acid sequences, still remain largely unexplored in this field. Read More

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Influence of the Polyacrylic Acid Binder Neutralization Degree on the Initial Electrochemical Behavior of a Silicon/Graphite Electrode.

ACS Appl Mater Interfaces 2021 Jun 8;13(24):28304-28323. Epub 2021 Jun 8.

Université de Nantes, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes F-44000, France.

The role of the physicochemical properties of the water-soluble polyacrylic acid (PAA) binder in the electrochemical performance of highly loaded silicon/graphite 50/50 wt % negative electrodes has been examined as a function of the neutralization degree in PAAHLi at the initial cycle in an electrolyte not containing ethylene carbonate. Electrode processing in the acidic PAAH binder at pH 2.5 leads to a deep copper corrosion, resulting in a significant electrode cohesion and adhesion to the current collector surface, but the strong binder rigidity may explain the big cracks occurring on the electrode surface at the first cycle. Read More

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