6,011 results match your criteria longissimus

Kohonen Artificial Neural Network and Multivariate Analysis in the Identification of Proteome Changes during Early and Long Aging of Bovine Muscle Using SWATH Mass Spectrometry.

J Agric Food Chem 2021 Sep 15. Epub 2021 Sep 15.

Department of Biotechnology, University of Verona, Strada le Grazie 15, Verona 37134, Italy.

To study proteomic changes involved in tenderization of , Charolais heifers and bulls muscles were sampled after early and long aging (12 or 26 days). Sensory evaluation and instrumental tenderness measurement were performed. Proteins were analyzed by gel-free proteomics. Read More

View Article and Full-Text PDF
September 2021

Effects of extended postmortem aging and intramuscular location on protein degradation, muscle fiber morphometrics, and tenderness of beef longissimus lumborum and semitendinosus steaks.

J Anim Sci 2021 Sep 13. Epub 2021 Sep 13.

University of Georgia, Department of Animal and Dairy Sciences, Athens, GA, USA.

The objective of this study was to determine effects of extended aging and intramuscular location on Warner-Bratzler shear force (WBSF), muscle fiber cross-sectional area (CSA), and protein degradation of semitendinosus (ST) and longissimus lumborum (LL) steaks. Left ST and LL were removed from 40 carcasses at 6 d postmortem. The ST was fabricated into 5 locations (LOC), with LOC 1 being most proximal and LOC 5 being most distal. Read More

View Article and Full-Text PDF
September 2021

The effects of age, sex, and hot carcass weight on cooked lamb flavor and off-flavor in four muscle cuts.

Transl Anim Sci 2021 Jul 10;5(3):txab083. Epub 2021 May 10.

Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.

The present study used 48 lambs originating from three different locations in the Western United States (16 lambs per location; 8 ewes and 8 wethers per location). Each consisting of similar breed composition (Suffolk cross) that were selected to represent weight by age at harvest treatments: light weight carcasses at 5 mo (, 31.81 ± 1. Read More

View Article and Full-Text PDF

Dietary Betaine Addition Alters Carcass Traits, Meat Quality, and Nitrogen Metabolism of Bama Mini-Pigs.

Front Nutr 2021 27;8:728477. Epub 2021 Aug 27.

Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Betaine is widely used as feed additives in animal husbandry as it can cause many benefits such as improving antioxidant ability, growth performance, and carcass traits. However, there are limited studies about the effects of betaine on the Bama mini-pigs. The present study was conducted to evaluate the effects of dietary betaine on carcass traits, meat quality, and nitrogen metabolism of pigs. Read More

View Article and Full-Text PDF

HMEJ-mediated site-specific integration of a myostatin inhibitor increases skeletal muscle mass in porcine.

Mol Ther Nucleic Acids 2021 Dec 24;26:49-62. Epub 2021 Jun 24.

Key Laboratory of Zoonosis Research, Ministry of Education, Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 130062 Changchun, Jilin Province, People's Republic of China.

As a robust antagonist of myostatin (), follistatin () is an important regulator of skeletal muscle development, and the delivery of to muscle tissue represents a potential therapeutic strategy for muscular dystrophies. The N terminus and FSI domain of are the functional domains for binding. Here, we aimed to achieve site-specific integration of FSI-I-I, including the signal peptide, N terminus, and three FSI domains, into the last codon of the porcine gene using a homology-mediated end joining (HMEJ)-based strategy mediated by CRISPR-Cas9. Read More

View Article and Full-Text PDF
December 2021

Effect of Gender to Fat Deposition in Yaks Based on Transcriptomic and Metabolomics Analysis.

Front Cell Dev Biol 2021 24;9:653188. Epub 2021 Aug 24.

Animal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China.

Fat deposition in yaks plays an important part in survival, multiplication, and meat quality. In this work, the characteristic of fat deposition in male yaks (MYs) and female yaks (FYs) and the regulations of gender to yak fat deposition were explored by mRNA-Seq and non-targeted metabolomics analyses. FYs possessed a higher body fat rate (BFR) of visceral fat, fat content in longissimus dorsi (LD) and liver, and subcutaneous fat thickness ( < 0. Read More

View Article and Full-Text PDF

Dietary calcium supplementation promotes the accumulation of intramuscular fat.

J Anim Sci Biotechnol 2021 Sep 10;12(1):94. Epub 2021 Sep 10.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Guangxi Zhuang Autonomous Region, Nanning, 530004, People's Republic of China.

Background: In the livestock industry, intramuscular fat content is a key factor affecting meat quality. Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism. However, few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation. Read More

View Article and Full-Text PDF
September 2021

miR-10a-5p Inhibits the Differentiation of Goat Intramuscular Preadipocytes by Targeting KLF8 in Goats.

Front Mol Biosci 2021 13;8:700078. Epub 2021 Aug 13.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China.

Intramuscular fat contributes to the improvement of meat quality of goats. MicroRNAs (miRNAs) have been reported to regulate adipocyte differentiation and maturation. The aim of our study was to clarify whether miR-10a-5p regulates goat intramuscular preadipocyte (GIPC) differentiation and its direct downstream signaling pathway. Read More

View Article and Full-Text PDF

Dynamic transcriptome profiles of postnatal porcine skeletal muscle growth and development.

BMC Genom Data 2021 Sep 6;22(1):32. Epub 2021 Sep 6.

Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong Province, China.

Background: Skeletal muscle growth and development are closely associated with the quantity and quality of pork production. We performed a transcriptomic analysis of 12 Longissimus dorsi muscle samples from Tibetan piglets at four postnatal stages of 0, 14, 30, and 60 days using RNA sequencing.

Results: According to the pairwise comparisons between the libraries of the muscle samples at the four postnatal stages, a total of 4115 differentially expressed genes (DEGs) were identified in terms of |log(fold change)| ≥ 1 and an adjusted P value < 0. Read More

View Article and Full-Text PDF
September 2021

The expression pattern, polymorphisms and association analyses of the porcine NREP gene.

J Anim Breed Genet 2021 Sep 6. Epub 2021 Sep 6.

Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.

NREP (neuronal regeneration related protein homolog) plays a role in the transformation of neural, muscle, and fibroblast cells and in smooth muscle myogenesis. The NREP gene was selected for detailed study as an expressional and functional candidate gene on the basis of data from the expression microarray, which detected the differences in gene expression between Czech Large White pigs and wild boars in the longissimus lumborum et thoracis and biceps femoris muscle tissues. Quantitative real-time PCR results confirmed that porcine NREP was expressed in both skeletal muscles and significantly overexpressed in Czech Large White pigs compared with wild boars (14. Read More

View Article and Full-Text PDF
September 2021

Seasons affect the phosphorylation of pork sarcoplasmic proteins related to meat quality.

Anim Biosci 2021 Aug 25. Epub 2021 Aug 25.

Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiangsu Synergetic Innovation Center of Meat Production, Processing and Quality Control; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Objective: Sarcoplasmic proteins include proteins that play critical roles in biological processes of living organisms. How seasons influence biological processes and meat quality of postmortem muscles through the regulation of protein phosphorylation remain to be investigated. In this study, the phosphorylation of sarcoplasmic proteins in pork longissimus muscle was investigated in four seasons. Read More

View Article and Full-Text PDF

Detection of contamination levels in fresh pork samples using electronic nose smellprints in tandem with support vector machine regression and metaheuristic optimization algorithms.

J Food Sci Technol 2021 Oct 16;58(10):3861-3870. Epub 2020 Oct 16.

School of Food and Biological Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013 Jiangsu People's Republic of China.

Rapid detection and quantification of bacterial foodborne pathogens are crucial in reducing the incidence of diseases associated with meat products contaminated with pathogens. For the identification, discrimination and quantification of contamination in pork samples, a commercial electronic nose with ten (10) metal oxide semiconductor sensor array is applied. Principal component analysis was successfully applied for discrimination of inoculated samples and inoculated samples at different contaminant levels. Read More

View Article and Full-Text PDF
October 2021

Regulating glycolysis and heat shock proteins in Gannan yaks ( ) in response to hypoxia of the Qinghai-Tibet Plateau.

Arch Anim Breed 2021 19;64(2):345-353. Epub 2021 Aug 19.

Faculty of Animal Science and Technology, Gansu Agricultural University, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070, China.

Glycolysis and heat shock proteins (HSPs) play an important role in hypoxia-intolerant species during hypoxia conditions. This study was conducted to evaluate the differences of glycolysis and heat shock proteins (HSPs) in Gannan yaks (), with the main goal of understanding how the response to hypoxia changes with altitude. Here, the genes and enzymes of glycolysis and HSPs were detected in heart, liver, lung, kidney, and longissimus dorsi from Gannan yaks at different altitude (2500 and 3500  ) using qPCR, western blot, and enzyme kits. Read More

View Article and Full-Text PDF

Impact of Exposure to Chronic Light-Dark Phase Shifting Circadian Rhythm Disruption on Muscle Proteome in Periparturient Dairy Cows.

Proteomes 2021 Jul 29;9(3). Epub 2021 Jul 29.

Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.

Muscle tissue serves as a key nutrient reservoir that dairy cows utilize to meet energy and amino acid requirements for fetal growth and milk production. Circadian clocks act as homeostatic regulators so that organisms can anticipate regular environmental changes. The objective of this study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine how chronic circadian disruption in late gestation affected the muscle tissue proteome. Read More

View Article and Full-Text PDF

Effect of bis-glycinate bound zinc or zinc sulfate on zinc metabolism in growing lambs.

J Anim Sci 2021 Aug 27. Epub 2021 Aug 27.

Department of Animal Science, Iowa State University College of Agriculture and Life Sciences, Ames, IA, United States.

To assess efficacy of bis-glycinate bound Zn, 36 crossbred wethers (34 ± 2 kg) were sorted by body weight into three groups and stagger started on a Zn deficient diet (18 mg Zn/kg dry matter; 22.5% neutral detergent fiber) for 45 d prior to a 15-d metabolism period (10 d adaptation, 5 d collection). On d 46, lambs were randomly assigned to dietary treatments (4 lambs treatment-1group -1): no supplemental Zn (CON) or 15 mg supplemental Zn/kg dry matter (ZINC) as Zn sulfate (ZS) or bis-glycinate (GLY; Plexomin Zn, Phytobiotics). Read More

View Article and Full-Text PDF

Comparison of Nutritional and Meat Quality Characteristics between Two Primal Cuts from Aceh Cattle in Aceh Province, Indonesia.

Vet Med Int 2021 17;2021:8381849. Epub 2021 Aug 17.

Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia.

The Aceh cattle are local Indonesian beef cattle that are farmed in Aceh Province. This type of cattle is one of the sources of meat for the Aceh people. This study aims to analyze the quality of two primal cuts (longissimus lumborum and semitendinosus muscle) from Aceh cattle based on the muscle microstructure characteristics and MSTN gene expression. Read More

View Article and Full-Text PDF

Sensory Consumer and Descriptive Analysis of Steaks from Beef Animals Selected from Tough and Tender Animal Genotypes: Genetic Meat Quality Traits Can Be Detected by Consumers.

Foods 2021 Aug 17;10(8). Epub 2021 Aug 17.

Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, P61 P302 Co. Cork, Ireland.

The objective of the present study was to determine if animals who were genetically divergent in the predicted tenderness of their meat actually produced more tender meat, as well as what the implications were for other organoleptic properties of the meat. The parental average genetic merit for meat tenderness was used to locate 20 "Tough genotype" heifers and 17 "Tender genotype" heifers; M. longissimus thoracis steaks from all heifers were subjected to sensory affective analysis (140 consumers) and sensory profiling using two trained sensory panels. Read More

View Article and Full-Text PDF

Effect of Dietary Brown Seaweed ( Additive on Meat Quality and Nutrient Composition of Fattening Pigs.

Foods 2021 Jul 26;10(8). Epub 2021 Jul 26.

I+D Patagonia Biotecnología SA., Valdivia 5090000, Chile.

The objective of this study was to evaluate the effects of dietary brown seaweed () additive (SWA) on meat quality and nutrient composition of commercial fattening pigs. The treatments were: Regular diet with 0% inclusion of SWA (CON); Regular diet with 2% SWA (2%-SWA); Regular diet with 4% SWA (4%-SWA). After slaughtering, five carcasses from each group were selected, and (LL) samples were taken for meat quality and chemical composition analysis. Read More

View Article and Full-Text PDF

In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes.

Genes (Basel) 2021 Aug 19;12(8). Epub 2021 Aug 19.

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Impaired skeletal muscle growth in utero can result in reduced birth weight and poor carcass quality in pigs. Recently, we showed the role of microRNAs (miRNAs) and their target genes in prenatal skeletal muscle development and pathogenesis of intrauterine growth restriction (IUGR). In this study, we performed an integrative miRNA-mRNA transcriptomic analysis in longissimus dorsi muscle (LDM) of pig fetuses at 63 days post conception (dpc) to identify miRNAs and genes correlated to fetal weight. Read More

View Article and Full-Text PDF

Effects of Ferulic Acid Supplementation on Growth Performance, Carcass Traits and Histochemical Characteristics of Muscle Fibers in Finishing Pigs.

Animals (Basel) 2021 Aug 20;11(8). Epub 2021 Aug 20.

Centro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Hermosillo 83304, Sonora, Mexico.

FA dietary supplementation on the growth performance, carcass traits and histochemical characteristics of the muscle from finishing pigs was investigated. Four hundred and twenty pigs were used in this study, and 105 animals (with five replicate pens and 21 pigs per pen) were assigned to one of four treatments: basal diet (BD) without additives (C-); BD + 10 ppm ractopamine hydrochloride + 0.97% lysine (C+); BD + 25 ppm of FA (FA); and BD + 25 ppm of FA + 0. Read More

View Article and Full-Text PDF

Effects of the Duration of Zilpaterol Hydrochloride Supplementation and Days on Feed on Performance, Carcass Traits and Saleable Meat Yield of Nellore Bulls.

Animals (Basel) 2021 Aug 20;11(8). Epub 2021 Aug 20.

Department of Animal Science, College of Agriculture, University of São Paulo, Luiz de Queiroz, Piracicaba 13418-900, SP, Brazil.

This study evaluated the effects of the duration of ZH supplementation and days on feed (DOF) on performance, carcass characteristics, and saleable meat yield of Nellore young bulls. The fixed effects included the duration (0, 20, 30, or 40 d before slaughter plus a 3 d ZH withdrawal period-8.33 mg of ZH/kg of DM) and DOF (90 and 117 d). Read More

View Article and Full-Text PDF

Purple Corn Anthocyanin Affects Lipid Mechanism, Flavor Compound Profiles, and Related Gene Expression of Muscle in Goats.

Animals (Basel) 2021 Aug 14;11(8). Epub 2021 Aug 14.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The current study aimed to investigate the effect of anthocyanins on muscle flavor compound profiles in goats. Goats in three groups were fed a basic diet or a diet supplemented with 0.5 g/d or 1 g/d anthocyanin-rich purple corn pigment (PCP). Read More

View Article and Full-Text PDF

Chickpea Can Be a Valuable Local Produced Protein Feed for Organically Reared, Native Bulls.

Animals (Basel) 2021 Aug 9;11(8). Epub 2021 Aug 9.

Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy.

We assessed the effects of inclusion of chickpea from 24 to 21%, as feed basis, in diets for organically reared bulls. Sixteen young bulls (270 ± 6.4 days of age; 246 ± 0. Read More

View Article and Full-Text PDF

Multi-Probiotic Supplementation Improves Liver Function and Reduces Cholesterol Levels in Jeju Native Pigs.

Animals (Basel) 2021 Aug 5;11(8). Epub 2021 Aug 5.

Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju-si 63243, Korea.

We evaluated the dietary effects of multiple probiotics in Jeju native pigs, using basal diet and multi-probiotic (basal diet with 1% multi-probiotics) treatments ( = 9 each) for 3 months. We analyzed growth performance, feed efficiency, backfat thickness, blood parameters, hematological profiles, adipokines, and immune-related cytokines in pig tissues. Average daily gain, feed intake, feed efficiency, backfat thickness, and body weight were not significantly different between both groups. Read More

View Article and Full-Text PDF

Effect of Wet Aging on Color Stability, Tenderness, and Sensory Attributes of and Muscles from Water Buffalo Bulls.

Animals (Basel) 2021 Jul 30;11(8). Epub 2021 Jul 30.

Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.

The present study aimed to investigate the effect of wet aging on meat quality characteristics of (LL) and (GM) muscles of buffalo bulls. Meat samples from six aging periods, i.e. Read More

View Article and Full-Text PDF

Relative Late Gestational Muscle and Adipose Thickness Reflect the Amount of Mobilization of These Tissues in Periparturient Dairy Cattle.

Animals (Basel) 2021 Jul 21;11(8). Epub 2021 Jul 21.

Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.

Due to insufficient dry matter intake and heightened nutrient requirements in early lactation, periparturient dairy cows mobilize adipose and muscle tissues to bridge energy and amino acid gaps, respectively. Our objective was to evaluate the relationship between the relative muscle thickness of late pregnant cows and their early lactation performance. At 35 d before expected calving (BEC), muscle thickness (LDT) was measured in forty-one multiparous Holstein cows via ultrasound. Read More

View Article and Full-Text PDF

Genetic analysis of growth, visual scores, height, and carcass traits in Nelore cattle.

Anim Sci J 2021 Dec;92(1):e13611

Department of Animal Science, Federal University of Pelotas, Pelotas, Brazil.

Covariance components were estimated for growth traits (BW, birth weight; WW, weaning weight; YW, yearling weight), visual scores (BQ, breed quality; CS, conformation; MS, muscling; NS, navel; PS, finishing precocity), hip height (HH), and carcass traits (BF, backfat thickness; LMA, longissimus muscle area) measured at yearling. Genetic gains were obtained and validation models on direct and maternal effects for BW and WW were fitted. Genetic correlations of growth traits with CS, PS, MS, and HH ranged from 0. Read More

View Article and Full-Text PDF
December 2021

Influence of aging time, temperature and relative humidity on the sensory quality of dry-aged Belgian Blue beef.

Meat Sci 2021 Aug 19;183:108659. Epub 2021 Aug 19.

Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Sciences and Aquatic Ecology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium. Electronic address:

The objective of this study was to evaluate the effect of aging period (0, 3, 6 or 9 weeks), aging temperature (2 versus 6 °C at 75% relative humidity, experiment 1) and relative humidity (70 versus 90% at 2 °C, experiment 2) on the sensory traits, oxidative stability and proteolysis of Belgian Blue beef. For each experiment, eight loins (M. longissimus thoracis et lumborum) from four animals (left and right side) were assigned to one of the two treatments (n = 4). Read More

View Article and Full-Text PDF

Why cumulative loading calculated using non-weighted integration may not be suitable for assessing physical stress of the lower back: an empirical investigation of strain during lifting and lowering tasks.

Ergonomics 2021 Aug 24:1-13. Epub 2021 Aug 24.

Chair and Institute of Industrial Engineering and Ergonomics, RWTH Aachen University, Aachen, Germany.

When work-related physical stress is assessed using non-weighted integration, it is assumed that different loading conditions have a sufficiently comparable effect on the human body as long as the area under the loading curve is the same. Growing evidence cast doubt on whether this simple calculation can adequately estimate physical work-related strain. This study investigates in vivo, focussing on the lower back, whether the non-weighted method adequately reflects work-related physical strain of the lower back. Read More

View Article and Full-Text PDF

Electrical Impedance to Easily Discover Undeclared Freeze-thaw Cycles in Slaughtered Bovine Meat.

J Electr Bioimpedance 2021 Jan 30;12(1):3-10. Epub 2021 Mar 30.

Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy.

A portable electrical impedance spectroscopy device was developed to monitor the bioimpedance resistive component of bovine meat by injecting a sinusoidal current of 1 mA at 65 kHz. Both right and left longissimus dorsi muscles were trimmed from 4 slaughtered cows. The left muscle portions were frozen to -18 °C for 7 days while the right ones were meantime maintained at 5 °C. Read More

View Article and Full-Text PDF
January 2021