12,814 results match your criteria long-term preservation


Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.

Sci Adv 2021 Jun 11;7(24). Epub 2021 Jun 11.

Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.

Space radiation may cause DNA damage to cells and concern for the inheritance of mutations in offspring after deep space exploration. However, there is no way to study the long-term effects of space radiation using biological materials. Here, we developed a method to evaluate the biological effect of space radiation and examined the reproductive potential of mouse freeze-dried spermatozoa stored on the International Space Station (ISS) for the longest period in biological research. Read More

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Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110.

Enzyme Microb Technol 2021 Aug 3;148:109811. Epub 2021 May 3.

Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan. Electronic address:

Microbial production of industrial chemicals is a sustainable approach to reduce the dependence on petroleum-based chemicals such as acids, alcohols, and amines, in which the cadaverine is a natural diamide and serves as one of the key monomers for biopolymer production. In this study, the constitutive promoter J23100 driven lysine decarboxylase (CadA) for cadaverine production was established and compared in different Escherichia coli strains. The best chassis designed as JW, expressed the highest amount of CadA by using J23100 promoter, showing stable and high copy numbers (i. Read More

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Empirical and Methodological Challenges to the Model-Based Inference of Diversification Rates in Extinct Clades.

Syst Biol 2021 Jun 10. Epub 2021 Jun 10.

Department of the Geophysical Sciences, University of Chicago, Chicago 60637, USA.

Changes in speciation and extinction rates are key to the dynamics of clade diversification, but attempts to infer them from phylogenies of extant species face challenges. Methods capable of synthesizing information from extant and fossil species have yielded novel insights into diversification rate variation through time, but little is known about their behavior when analyzing entirely extinct clades. Here, we use empirical and simulated data to assess how two popular methods, PyRate and Fossil BAMM, perform in this setting. Read More

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Long-term outcomes after pyeloplasty for pelvi-ureteric junction obstruction in adults associated with renal congenital anomalies: Age, sex and renal function matched analysis.

Arab J Urol 2020 Sep 2;19(2):173-178. Epub 2020 Sep 2.

Department of Urology, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.

: To assess the long-term outcomes after pyeloplasty for pelvi-ureteric junction obstruction (PUJO) associated with renal anomalies. : Data were collected for patients after pyeloplasty for PUJO associated with renal anomalies and analysed retrospectively. Long-term functional success was evaluated by comparing the renographic split renal function (SRF) and glomerular filtration rate (GFR) at last follow-up with baseline values. Read More

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September 2020

Patients' and Providers' Needs and Preferences When Considering Fertility Preservation Before Cancer Treatment: Decision-Making Needs Assessment.

JMIR Form Res 2021 Jun 7;5(6):e25083. Epub 2021 Jun 7.

Department of Gynecological Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, United States.

Background: As cancer treatments continue to improve, it is increasingly important that women of reproductive age have an opportunity to decide whether they want to undergo fertility preservation treatments to try to protect their ability to have a child after cancer. Clinical practice guidelines recommend that providers offer fertility counseling to all young women with cancer; however, as few as 12% of women recall discussing fertility preservation. The long-term goal of this program is to develop an interactive web-based patient decision aid to improve awareness, access, knowledge, and decision making for all young women with cancer. Read More

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Anastomosis near to the ileocecal valve in neonates with focal intestinal perforation, is it safe.

J Matern Fetal Neonatal Med 2021 Jun 6:1-4. Epub 2021 Jun 6.

Neonatal Surgery Unit, Pediatric Surgery Division, Vall d'Hebron University Hospital, Barcelona, Spain.

Background: Anastomosis near the ileocecal valve (ICV) are controversial due to the increased pressure on the suture; in this situation, the valve could be removed at a first stage or at the moment of stoma closure. However, preservation of the ICV has proved important benefits in the long term. The aim of this study is to evaluate its feasibility in neonates with focal intestinal perforation (FIP). Read More

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The Long-Term Presence of SARS-CoV-2 on Cold-Chain Food Packaging Surfaces Indicates a New COVID-19 Winter Outbreak: A Mini Review.

Front Public Health 2021;9:650493. Epub 2021 May 20.

General Practice Teaching and Research Section, Weifang Medical University, Weifang, China.

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is a highly infectious virus that is transmitted primarily through droplets or by coming in close contact with an infected person. In 2020, there was a global outbreak of COVID-19, resulting in an unprecedented global burden of disease, health care costs, and had a significant economic impact. Recently, SARS-CoV-2 was detected on the outer packaging of imported cold chain items in China and has led to virus transmission events, causing great concern. Read More

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Oncological and Functional Outcomes in Joint-sparing Resections of the Proximal Femur for Malignant Primary Bone Tumors.

J Pediatr Orthop 2021 Jun 7. Epub 2021 Jun 7.

Department of Orthopaedic Surgery, Boston Children's Hospital Department of Orthopaedic Surgery, Massachusetts General Hospital Section of Orthopaedic Oncology, Division of Orthopaedic Surgery, Lahey Hospital and Medical Center, Burlington Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Boston, MA Department of Orthopaedic Surgery, Tripler Army Medical Center, Honolulu, HI Heart and Vascular Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH Allegheny Orthopaedic Associates, Pittsburgh, PA.

Background: Joint-sparing resections (JSR) of the proximal femur allow for preservation of the proximal femoral growth plate and native hip joint, but whether this offers fewer complications or better function and longevity of the reconstruction remains unknown. In this study, we compared the functional outcomes of pediatric patients with bone sarcomas undergoing JSR of the proximal femur with intercalary allograft (ICA) reconstruction to those undergoing proximal femoral resections (PFR) with allograft-prosthetic composite (APC) reconstructions.

Methods: We retrospectively reviewed all patients undergoing JSR with ICA reconstruction and PFR with APC reconstructions between 1995 and 2013 at a tertiary pediatric referral center. Read More

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Short-Term Outcomes After Spleen-Preserving Minimally Invasive Distal Pancreatectomy With or Without Preservation of Splenic Vessels: A Pan-European Retrospective Study in High-Volume Centers.

Ann Surg 2021 Jun 2. Epub 2021 Jun 2.

Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, the Netherlands Department of General Surgery, Instituto Ospedaliero Fondazione Poliambulanza, Brescia, Italy Department of Surgery, Morriston Hospital, Swansea, United Kingdom Department of Surgery and Department of Biomedical and Clinical Sciences, Linköping University, Sweden Division of General and Transplant Surgery, University of Pisa, Pisa, Italy Department of Surgery, Pederzoli Hospital, Peschiera, Italy Department of Surgery, S. Orsola-Malpighi Hospital, Bologna, Italy Department of Surgery, Pancreas Institute, Verona University Hospital, Verona, Italy Department of Surgery, Hopital Saint Eloi, Montpellier, France Department of Surgery, Niguarda Ca' Granda Hospital, Milan, Italy Department of Surgery, Hospital of Beaujon, Clichy, France Department of Surgery, Royal Free Hospital NHS Foundation Trust, London, United Kingdom Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, the Netherlands Department of Surgery, Heidelberg University Hospital, Heidelberg, Germany Department of Digestive and Hepatobiliary/Pancreatic Surgery, Groeninge Hospital, Kortrijk, Belgium Department of Hepatobiliary and Pancreatic Surgery, Addenbrooke's Hospital, Cambridge, UK Department of Surgery, University Hospital Schleswig Holstein, Campus Lübeck, Lübeck, Germany Emergency and General Surgery Department, Azienda Ospedaliera Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy and General Surgery Department, Policlinico Abano Terme, Italy Department of Surgery, Regional Academic Cancer Center Utrecht, UMC Utrecht Cancer Center and St Antonius Hospital Nieuwegein, Utrecht University, Utrecht, the Netherlands Department of Visceral and Digestive Surgery, Nouvel Hôpital Civil, University hospital of Strasbourg Department of Surgery, University hospital Pavia, Pavia, Italy The Intervention Center, Department of HPB Surgery, Department of Research & Development, Division of Emergencies and Critical Care Oslo University Hospital and Institute for Clinical Medicine, University of Oslo, Norway Department of Research & Development, Division of Emergencies and Critical Care, Oslo University Hospital Department of Surgery, Oxford University Hospital NHS Foundation Trust, Oxford, United Kingdom Department of Surgery, the Freeman Hospital Newcastle Upon Tyne, Newcastle, United Kingdom Pancreatic Surgery, Humanitas IRCCS, Rozzano and Humanitas University, Department of Biomedical Sciences, Milan, Italy Department of Surgery, Southampton University Hospital NHS Foundation Trust, Southampton, United Kingdom.

Objective: To compare short-term clinical outcomes after Kimura and Warshaw minimally invasive distal pancreatectomy (MIDP).

Background: Spleen preservation during distal pancreatectomy can be achieved by either preservation (Kimura) or resection (Warshaw) of the splenic vessels. Multicenter studies reporting outcomes of Kimura and Warshaw spleen-preserving MIDP are scarce. Read More

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Auricular reconstruction after Mohs micrographic surgery: analysis of 101 cases.

An Bras Dermatol 2021 Jun 2. Epub 2021 Jun 2.

Dermatology Service, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil; Postgraduate Program in Internal Medicine and Health Sciences, Universidade Federal do Paraná, Curitiba, PR, Brazil; Private Practice, Curitiba, PR, Brazil.

Background: The ear is a region that has a high prevalence of cutaneous carcinomas and several guidelines indicate Mohs micrographic surgery as the first-choice treatment in such cases. Although the technique allows maximum preservation of healthy tissue, many auricular surgical wounds constitute a challenge due to the peculiar local anatomy, with evident curves and reliefs. Auricular reconstruction should prioritize function before aesthetics, but without leaving the latter aside, since postoperative distortions can have a significant psychological impact. Read More

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Current Awareness and Attitude toward Fertility Preservation in Indonesia: A Nationwide Survey Among Health-care Providers.

J Hum Reprod Sci 2021 Jan-Mar;14(1):81-86. Epub 2021 Mar 30.

Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Context: Fertility preservation (FP) is necessary for cancer patients who develop infertility due to chemotherapy and radiotherapy. In Indonesia, many systematic steps and long-term continued actions must be taken to establish FP units since there has been an increasing incidence of cancer among people under 45 years old.

Aims: This study aimed at a comprehensive evaluation on the awareness and practices of health-care providers (HCPs) toward FP. Read More

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The Role of Quantic Molecular Resonance (QMR) in the Treatment of Inferior Turbinate Hypertrophy (ITH): Our Experience With Long-Term Follow-Up in Allergic and Nonallergic Rhinitis Refractory to Medical Therapy. Preliminary Results.

Ear Nose Throat J 2021 Jun 3:1455613211001599. Epub 2021 Jun 3.

Ear Nose and Throat Unit, Azienda 18617Ospedaliera di Rilievo Nazionale Antonio Cardarelli, vi Antonio Caradelli, Napoli, Italy.

Objective: The aim of this study was to assess the long-term effectiveness of quantic molecular resonance (QMR) in the treatment of inferior turbinate hypertrophy (ITH) in allergic and nonallergic rhinitis refractory to medical therapy.

Methods: This study enrolled 281 patients, 160 males (56.9%) and 121 females (43. Read More

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Comparing the Gonadotoxicity of Multiple Breast Cancer Regimens: Important Understanding for Managing Breast Cancer in Pre-Menopausal Women.

Breast Cancer (Dove Med Press) 2021 24;13:341-351. Epub 2021 May 24.

Department of Medical Oncology, U.O.C Clinica Di Oncologia Medica, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Over the last several decades, improvements in breast cancer treatment have contributed to increased cure rates for women diagnosed with this malignancy. Consequently, great importance should be paid to the long-term side effects of systemic therapies. For young women (defined as per guideline ≤40 years at diagnosis) who undergo chemotherapy, one of the most impactful side effects on their quality of life is premature ovarian insufficiency (POI) leading to fertility-related problems and the side effects of early menopause. Read More

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Long-term preservation of pharyngeal swallowing function in MM2-cortical-type sporadic Creutzfeldt-Jakob disease.

Prion 2021 Dec;15(1):82-86

Department of Neurology, Gifu University Graduate School of Medicine, Gifu, Japan.

Swallowing function in long-term survivors of Creutzfeldt-Jakob disease (CJD) has not been elucidated. Herein, we report a patient with MM2-cortical-type sporadic CJD (MM2C-type sCJD) with long-term preservation of pharyngeal swallowing function using videofluoroscopic (VF) examination of swallowing. A 55-year-old woman was admitted to hospital because of dyscalculia and memory disturbance 3 years after the onset of these symptoms. Read More

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December 2021

Surface functionalized inorganic phosphor by grafting organic antenna for long term preservation of latent fingerprints and data-security applications.

J Colloid Interface Sci 2021 May 11;600:887-897. Epub 2021 May 11.

Prof. C.N.R. Rao Centre for Advanced Materials, Tumkur University, Tumkur 572 103, India. Electronic address:

Creative advancements are enormously sought for the advanced forensic and data security in modern era. Herein, fabrication of Bovine Serum Albumin (BSA) functionalized GdO:Eu (5 mol %) nanopowders dispersed in a poly(vinyl alcohol) (PVA) matrix for long term preservation and visualization of latent fingerprints, as well as printing. Efficient intramolecular energy transfers from coordinated ligand to the doped Eu ions, called the antenna effect was precisely organized by grafting organic molecule, resultant to an enhanced photoluminescence emission. Read More

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Day-Time Declamping Is Associated with Better Outcomes in Kidney Transplantation: The Circarein Study.

J Clin Med 2021 May 26;10(11). Epub 2021 May 26.

CHU Lille, 59000 Lille, France.

Despite improvements in organ preservation techniques and efforts to minimize the duration of cold ischemia, ischemia-reperfusion (IR) injury remains associated with poor graft function and long-term survival in kidney transplantation. We recently demonstrated a clinically significant day-time variation in myocardial tolerance to IR, transcriptionally orchestrated by the circadian clock. Patient and graft post-transplant survival were studied in a cohort of 10,291 patients first transplanted between 2006 and 2017 to test whether kidney graft tolerance to IR depends on the time-of-the-day of clamping/declamping, and thus impacts graft and patient survival. Read More

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Donor Heart Preservation with Hydrogen Sulfide: A Systematic Review and Meta-Analysis.

Int J Mol Sci 2021 May 27;22(11). Epub 2021 May 27.

University Medical Centre Groningen, Department of Cardiothoracic Surgery, University of Groningen, 9700 RB Groningen, The Netherlands.

Preclinical studies have shown that postconditioning with hydrogen sulfide (HS) exerts cardioprotective effects against myocardial ischemia-reperfusion injury (IRI). The aim of this study was to appraise the current evidence of the cardioprotective effects of HS against IRI in order to explore the future implementation of HS in clinical cardiac transplantation. The current literature on HS postconditioning in the setting of global myocardial ischemia was systematically reviewed and analyzed, performing meta-analyses. Read More

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Long-Term Preservation and Storage of Faecal Samples in Whatman Cards for PCR Detection and Genotyping of and .

Animals (Basel) 2021 May 12;11(5). Epub 2021 May 12.

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28220 Madrid, Spain.

Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, and . Three commercially available Whatman Filter Cards were comparatively assessed: the FTA Classic Card, the FTA Elute Micro Card, and the 903 Protein Saver Card. Read More

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Autolysis and Cell Death Is Affected by pH in DSM 20016 Cells.

Foods 2021 May 9;10(5). Epub 2021 May 9.

Food Microbiology and Biotechnology Laboratory, Food and Nutritional Sciences Program, 163 Carver Hall, North Carolina A&T State University, 1601 Market St., Greensboro, NC 27411, USA.

A key obstacle to the successful delivery of a probiotic to the consumer is maintaining viability of the live cells during storage, a challenge for the beneficial . Three processes play a role in the reduction of viability: autolysis, cell death, and cell weakening. Using a phosphate induction model of autolysis, the initial aim of this project was to discover novel molecular determinants of autolysis in , with the long -term goal of elucidating new strategies for increasing viability. Read More

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The Kynurenic Acid Analog SZR72 Enhances Neuronal Activity after Asphyxia but Is Not Neuroprotective in a Translational Model of Neonatal Hypoxic Ischemic Encephalopathy.

Int J Mol Sci 2021 May 1;22(9). Epub 2021 May 1.

Department of Physiology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary.

Hypoxic-ischemic encephalopathy (HIE) remains to be a major cause of long-term neurodevelopmental deficits in term neonates. Hypothermia offers partial neuroprotection warranting research for additional therapies. Kynurenic acid (KYNA), an endogenous product of tryptophan metabolism, was previously shown to be beneficial in rat HIE models. Read More

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Multidisciplinary Clinical Considerations in the Treatment of Pediatric Epithelial Ovarian Cancer.

J Pediatr Adolesc Gynecol 2021 May 29. Epub 2021 May 29.

University of Michigan Department of Obstetrics and Gynecology.

Background: Epithelial ovarian cancers are a rare subset of the <1% of ovarian cancers diagnosed in children. This case highlights considerations when caring for these patients.

Case: Evaluation of a 12-year-old post-menarchal girl presenting with suprapubic pain revealed a solid/cystic pelvic mass involving bilateral adnexa and elevated CA-125. Read More

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Materials engineering of violin soundboards by Stradivari and Guarneri.

Angew Chem Int Ed Engl 2021 Jun 1. Epub 2021 Jun 1.

National Taiwan University, Chemistry, 1 Roosevelt Road Sec 4, 106, Taipei, TAIWAN.

Leading violinists today still prefer violins made three centuries ago by Stradivari and Guarneri in Cremona, Italy. Their unique qualities remain an unsolved puzzle, including the long-term durability of soundboards at reduced thickness. Here, we investigated the material properties of Cremonese soundboards using a wide range of spectroscopic, microscopic, and chemical techniques. Read More

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Amelioration of lipopolysaccharide-induced memory impairment in equilibrative nucleoside transporter-2 knockout mice is accompanied by the changes in glutamatergic pathways.

Brain Behav Immun 2021 May 28. Epub 2021 May 28.

School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan. Electronic address:

Neuroinflammation has been implicated in cognitive deficits in neurological and neurodegenerative diseases. Lipopolysaccharide (LPS)-induced neuroinflammation and the breakdown of the blood-brain barrier can be attenuated in mice with equilibrative nucleoside transporter-2 (ENT2/Ent2) deletion. The present study was aimed to investigate the role of ENT2 in cognitive and neuronal functions under physiological and inflammatory conditions, in terms of behavioral performance and synaptic plasticity in saline- and LPS-treated Ent2 knockout (KO) mice and their wild-type (WT) littermate controls. Read More

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Contact X-ray brachytherapy for rectal cancer: Past, present, and future.

Cancer Radiother 2021 May 26. Epub 2021 May 26.

Centre Antoine-Lacassagne, 33, avenue de Valombrose, 06000 Nice, France.

The Papillon experience and the Lyon R96-02 trial have shown that contact X-ray brachytherapy of 50kV is efficient and safe to achieve long term local control and organ preservation for cT1 and early cT2-3 rectal cancers. The OPERA trial, using the Papillon 50™ machine, brings further support to this preservation strategy for selected T2T3ab lesions. Future trials using a contact X-ray boost will try to consolidate and enlarge its place in organ preservation for rectal cancers. Read More

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Long-term (16-26 years) follow-up outcome of steroid therapy in refractory autoimmune sensorineural hearing loss.

J Autoimmun 2021 Jul 25;121:102664. Epub 2021 May 25.

Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, 634-8522, Japan.

Autoimmune sensorineural hearing loss (ASHL) is a rare disease of uncertain etiology, with no established treatment strategy. The duration of morbidity is increased in refractory cases; and therefore, the preservation of hearing and the prevention of adverse effects with steroid therapy are serious long term issues to consider. Long-term follow up of patients treated for ASHL was performed retrospectively in order to elucidate the pathogenesis of ASHL, evaluate the consequences of steroid therapy, and determine a promising treatment course. Read More

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A Roadmap to Cardiac Tissue-Engineered Construct Preservation: Insights from Cells, Tissues, and Organs.

Adv Mater 2021 May 28:e2008517. Epub 2021 May 28.

Department of Cardiology, Experimental Cardiology Laboratory, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands.

Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue-engineered (cTE) constructs. These patient-specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. Read More

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Trehalose Attenuates Detrimental Effects of Freeze-Drying on Human Sperm Parameters.

Biopreserv Biobank 2021 May 27. Epub 2021 May 27.

Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Freeze-drying is one of the sperm preservation methods leading to the long-term preservation of sperm genetic material. Our main goal of this study was to evaluate the effect of the trehalose freeze-drying method on sperm motility, viability, morphology, acrosome, and DNA integrity compared with a standard protocol without trehalose. Twenty-five normozoospermic samples were included in this prospective study. Read More

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Primary outcomes of a femoral neck-preserving stem: a multicentre clinical-radiological analysis at 5-year follow-up.

Eur J Orthop Surg Traumatol 2021 May 26. Epub 2021 May 26.

Orthopedic and Traumatology Service , Hospital Italiano de Buenos Aires, Buenos Aires, Argentina.

Purpose: The aim of this prospective study was to evaluate the clinical-radiological outcomes of the Alteon Neck Preserving Stem (ANPS) (Exactech, Gainesville, FL, USA) implanted in four different centres by five senior hip surgeons with a minimum of 5 years of follow-up.

Material And Method: We conducted a multicentre prospective study that analysed 155 consecutive total hip replacements during 2014. We performed clinical-radiological analysis measuring Harris Hip Score (HHS), subjective satisfaction evaluation, radiolucencies, position of components, limb length discrepancy, heterotopic ossification, medical and surgical complications and a survival analysis at 5 years follow-up. Read More

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[Long-term Storage and Rapid Activity Recovery of ANAMMOX Granular Sludge].

Huan Jing Ke Xue 2021 Jun;42(6):2957-2965

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, China.

At 4℃ and with no substrate, the activity recovery of ANAMMOX granular sludge was examined after 230 days of storage, and the effect of adding two organic carbon sources (glucose and sodium propionate) on the recovery was explored. After 230 days of long-term storage, the activity of ANAMMOX bacteria was 0.013 g·(g·d), which was just 6. Read More

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Bone fragment or bone powder? ATR-FTIR spectroscopy-based comparison of chemical composition and DNA preservation of bones after 10 years in a freezer.

Int J Legal Med 2021 May 24. Epub 2021 May 24.

National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia.

Freezing bone samples to preserve their biomolecular properties for various analyses at a later time is a common practice. Storage temperature and freeze-thaw cycles are well-known factors affecting degradation of molecules in the bone, whereas less is known about the form in which the tissue is most stable. In general, as little intervention as possible is advised before storage. Read More

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