Cell Calcium 2021 Apr 8;96:102406. Epub 2021 Apr 8.
Department of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street 16/10, Moscow, 117997, Russia; Department of Physiology, Jiaxing University College of Medicine, Zhejiang, 314033 China; Sechenov First Moscow State Medical University, Bolshaya Pirogovskaya Str 19с1, Moscow, 119146, Russia. Electronic address:
The effect of brain extracellular matrix (ECM) on synaptic plasticity remains controversial. Here, we show that targeted enzymatic attenuation with chondroitinase ABC (ChABC) of ECM triggers the appearance of new glutamatergic synapses on hippocampal pyramidal neurons, thereby increasing the amplitude of field EPSPs while decreasing both the mean miniature EPSC amplitude and AMPA/NMDA ratio. Although the increased proportion of 'unpotentiated' synapses caused by ECM attenuation should promote long-term potentiation (LTP), surprisingly, LTP was suppressed. Read More