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LINC01232 Promotes Metastasis and EMT by Regulating miR-506-5p/PAK1 Axis in Gastric Cancer.

Cancer Manag Res 2022 13;14:1729-1740. Epub 2022 May 13.

Digestive System Department, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, People's Republic of China.

Background: Long non-coding RNA LINC01232 plays an important role in the progression of metastasis in several cancers. However, the function of LINC01232 in gastric cancer is limited. Authors aimed to investigate the role and mechanism of LINC01232 in the metastasis of gastric cancer. Read More

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CircLIFR suppresses hepatocellular carcinoma progression by sponging miR-624-5p and inactivating the GSK-3β/β-catenin signaling pathway.

Cell Death Dis 2022 May 17;13(5):464. Epub 2022 May 17.

Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 510120, Guangzhou, China.

Circular RNAs have been reported to play essential roles in the tumorigenesis and progression of various cancers. However, the biological processes and mechanisms involved in hepatocellular carcinoma (HCC) remain unclear. Initial RNA-sequencing data and qRT-PCR results in our cohort showed that hsa_circ_0072309 (also called circLIFR) was markedly downregulated in HCC tissues. Read More

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Functional connectivity maps of theta/alpha and beta coherence within the subthalamic nucleus region.

Neuroimage 2022 May 14:119320. Epub 2022 May 14.

Department of Neurology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

The subthalamic nucleus (STN) is a primary target for deep brain stimulation in Parkinson's disease (PD). Although small in size, the STN is commonly partitioned into sensorimotor, cognitive/associative, and limbic subregions based on its structural connectivity profile to cortical areas. We investigated whether such a regional specialization is also supported by functional connectivity between local field potential recordings and simultaneous magnetoencephalography. Read More

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The WKRY transcription factor MdWRKY75 regulates anthocyanins accumulation in apples (Malus domestica).

Funct Plant Biol 2022 May 17. Epub 2022 May 17.

Anthocyanins play important roles in plant secondary metabolism. Although previous studies have identified many transcription factors (TFs) that participate in the synthetic pathway of anthocyanins, the regulation mechanism of the pathway remain poorly understood. In this study, we identified a WRKY Group IIc TF, MdWRKY75, which contained a typical WRKYGQK heptapeptide sequence and a C2H2-zinc finger structure. Read More

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The Role of Extracellular Matrix Components in the Spreading of Pathological Protein Aggregates.

Front Cell Neurosci 2022 29;16:844211. Epub 2022 Apr 29.

UK Dementia Research Institute, University College London, London, United Kingdom.

Several neurodegenerative diseases are characterized by the accumulation of aggregated misfolded proteins. These pathological agents have been suggested to propagate in the brain mechanisms similar to that observed for the prion protein, where a misfolded variant is transferred from an affected brain region to a healthy one, thereby inducing the misfolding and/or aggregation of correctly folded copies. This process has been characterized for several proteins, such as α-synuclein, tau, amyloid beta (Aβ) and less extensively for huntingtin and TDP-43. Read More

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Ribosomal DNA transcription is increased in the left nucleus accumbens of heroin-dependent males.

Eur Arch Psychiatry Clin Neurosci 2022 May 14. Epub 2022 May 14.

Department of Forensic Medicine, Medical University of Gdańsk, ul. Dębowa 23, 80-204, Gdańsk, Poland.

Opioid addiction is a worldwide problem accentuated in the USA and European countries by the COVID-19 pandemic. The nucleus accumbens (NAc) plays an outstanding neurobiological role in opioid addiction as a part of the striatum and key component of brain reward system. The striatal GABAergic medium spiny projection neurons (MSNs) are the main neuronal type in the NAc where addiction-specific synaptic plasticity occurs. Read More

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The brain of the tree pangolin (Manis tricuspis). VII. The amygdaloid body.

J Comp Neurol 2022 May 14. Epub 2022 May 14.

School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Republic of South Africa.

Here, we describe the cytoarchitecture and chemoarchitecture of the amygdaloid body of the tree pangolin. Our definition of the amygdaloid body includes the pallial portions of the amygdala, and the centromedial group that is a derivative of the subpallium and part of the extended amygdala. The remainder of the extended amygdala is not described herein. Read More

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Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Nodulation.

Int J Mol Sci 2022 May 9;23(9). Epub 2022 May 9.

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca 62210, Mexico.

The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes place by inducing cortical cell division (CCD) at the infection site. Read More

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The Nature of Noradrenergic Volume Transmission From Locus Coeruleus to Brainstem Mesencephalic Trigeminal Sensory Neurons.

Front Cell Neurosci 2022 26;16:841239. Epub 2022 Apr 26.

Department of Brain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.

Noradrenergic neurons in the locus coeruleus (LC) release noradrenaline (NA) that acts volume transmission to activate extrasynaptic G-protein coupled receptors (GPCRs) in target cells throughout the brain. As the closest projection, the dorsal LC laterally adjoins the mesencephalic trigeminal nucleus (MTN), in which proprioceptive primary sensory neurons innervating muscle spindles of jaw-closing muscles are exceptionally located. MTN neurons express α-adrenergic receptors (α-ARs) and display hyperpolarization-activated cyclic nucleotide-gated (HCN) currents (Ihs), which is downregulated by α-AR activation. Read More

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BcWRKY1 confers salt sensitivity via inhibiting Reactive oxygen species scavenging.

Plant Mol Biol 2022 May 12. Epub 2022 May 12.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.

WRKY transcription factors play important roles in abiotic stress by directly regulating stress-related genes. However, the molecular mechanism of its involvement in salt stress in pak-choi is still poorly understood. In this study, we elucidated the function of BcWRKY1 from pak-choi (Brassica rapa ssp. Read More

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Identification of , a QTL Coordinately Controls Grain Size, Grain Number per Panicle, and Grain Weight in Rice.

Front Plant Sci 2022 25;13:880919. Epub 2022 Apr 25.

Chinese National Center for Rice Improvement and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.

Grain size, grain number per panicle, and grain weight are key agronomic traits that determine grain yield in rice. However, the molecular mechanisms coordinately controlling these traits remain largely unknown. In this study, we identified a major QTL, , that is responsible for grain size, grain number per panicle, and grain weight in rice, which encodes a MYB-like protein. Read More

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Sweet spots of standard and directional leads in patients with refractory essential tremor: white matter pathways associated with maximal tremor improvement.

J Neurosurg 2022 Apr 29:1-10. Epub 2022 Apr 29.

5Department of Neurosurgery, Cantonal Hospital St. Gallen, Switzerland; and.

Objective: In patients with essential tremor (ET) treated with standard deep brain stimulation (sDBS) whose ET had progressed and who no longer received optimal benefit from sDBS, directional deep brain stimulation (dDBS) may provide better tremor control. Current steering may provide better coverage of subcortical structures related to tremor control in patients with ET and significant progression without optimal response to sDBS.

Methods: This study included 6 patients with ET initially treated with sDBS whose tremor later progressed and who then underwent reimplantation with dDBS to optimize their tremor control. Read More

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Halting ErbB-2 isoforms retrograde transport to the nucleus as a new theragnostic approach for triple-negative breast cancer.

Cell Death Dis 2022 May 9;13(5):447. Epub 2022 May 9.

Laboratory of Molecular Mechanisms of Carcinogenesis and Molecular Endocrinology, Instituto de Biología y Medicina Experimental (IBYME), CONICET, Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina.

Triple-negative breast cancer (TNBC) is clinically defined by the absence of estrogen and progesterone receptors and the lack of membrane overexpression or gene amplification of receptor tyrosine kinase ErbB-2/HER2. Due to TNBC heterogeneity, clinical biomarkers and targeted therapies for this disease remain elusive. We demonstrated that ErbB-2 is localized in the nucleus (NErbB-2) of TNBC cells and primary tumors, from where it drives growth. Read More

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Control of Behavioral Arousal and Defense by a Glutamatergic Midbrain-Amygdala Pathway in Mice.

Front Neurosci 2022 18;16:850193. Epub 2022 Apr 18.

Department of Epidemiology and Health Statistics, The School of Public Health, Fujian Medical University, Fuzhou, China.

In response to external threatening signals, animals evolve a series of defensive behaviors that depend on heightened arousal. It is believed that arousal and defensive behaviors are coordinately regulated by specific neurocircuits in the central nervous system. The ventral tegmental area (VTA) is a key structure located in the ventral midbrain of mice. Read More

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Circadian clocks, cognition, and Alzheimer's disease: synaptic mechanisms, signaling effectors, and chronotherapeutics.

Mol Neurodegener 2022 May 7;17(1):35. Epub 2022 May 7.

Department of Neuroscience, Ohio State University, Graves Hall, 333 W. 10th Ave, Columbus, OH, 43210, USA.

Modulation of basic biochemical and physiological processes by the circadian timing system is now recognized as a fundamental feature of all mammalian organ systems. Within the central nervous system, these clock-modulating effects are reflected in some of the most complex behavioral states including learning, memory, and mood. How the clock shapes these behavioral processes is only now beginning to be realized. Read More

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Functional and Structural Changes in the Membrane-Bound O-Acyltransferase Family Member 7 (MBOAT7) Protein: The Pathomechanism of a Novel Variant in Patients With Intellectual Disability.

Front Neurol 2022 18;13:836954. Epub 2022 Apr 18.

Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.

The membrane-bound O-acyltransferase domain-containing 7 gene is associated with intellectual disability, early onset seizures, and autism spectrum disorders. This study aimed to determine the pathogenetic mechanism of the missense variant via molecular modeling. Three patients from a consanguineous family were found to have a homozygous c. Read More

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Analysis of collateral projections from the lateral orbitofrontal cortex to nucleus accumbens and basolateral amygdala in rats.

J Neurophysiol 2022 May 4. Epub 2022 May 4.

Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan.

The orbitofrontal cortex (OFC) is an important brain area for executive functions. The OFC projects to both the nucleus accumbens (NAc) and the basolateral nucleus of the amygdala (BLA). These two pathways share some similar behavioral functions, but their anatomical and physiological properties have not been compared before. Read More

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rhEPO Upregulates the PPARγ Pathway in Long-term Cultured Primary Nerve Cells via PI3K/Akt to Delay Cell Senescence.

J Mol Neurosci 2022 May 3. Epub 2022 May 3.

Department of Neurology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.

Previous studies have confirmed that both recombinant human erythropoietin (rhEPO) and peroxisome proliferator-activated receptors γ (PPARγ) activator pioglitazone can protect senescent nerve cells, and their mechanisms involve enhancing cell antioxidant capacity and reducing cell apoptosis. However, whether the PPARγ pathway is involved in the rhEPO anti-aging process in neuronal cells is still unclear. In this study, to explore the relationship between rhEPO and the PPARγ pathway at the cellular level, primary nerve cells cultured for 22 days were used to simulate the natural aging process of nerve cells. Read More

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Tightly Focused Femtosecond Laser Radiation Induces DNA Double-Strand Breaks in Human Tumor Cells.

Bull Exp Biol Med 2022 Apr 2;172(6):743-746. Epub 2022 May 2.

N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.

We studied the formation of double-strand DNA breaks (DNA DSB) induced by femtosecond laser radiation in A549 human lung adenocarcinoma cells using immunocytochemical staining of the resulting tracks of a specific DSB marker protein phosphorylated ATM kinase (phospho-ATM). Additionally, colocalization of phospho-ATM tracks with γH2AX protein tracks was studied. The results of immunocytochemical analysis showed that 30 min after irradiation of cells with femtosecond pulses with energies of 1 and 2 nJ (radiation power density 2×10 and 4×10 W×cm, respectively), the formation of tracks consisting of phospho-ATM and γH2AX proteins located in sites where the laser beam passes through the cell nuclei was observed. Read More

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Development of neurochemical labeling in the intermediolateral nucleus of cats' spinal cord.

Anat Rec (Hoboken) 2022 May 2. Epub 2022 May 2.

Laboratory of Neuromorphology, Pavlov Institute of Physiology RAS, Saint-Petersburg, Russia.

NeuN is a neuron-specific nuclear protein expressed in most mature neuronal cell types, with some exceptions. These exceptions are known mainly for the brain but not for the spinal cord or the spinal visceral networks for which only scarce information is available. One of the most defined visceral structures in the spinal cord is the sympathetic intermediolateral nucleus located within the thoracolumbar segments. Read More

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General regulatory factor 3 regulates the expression of alternative oxidase 1a and the biosynthesis of glucosinolates in cytoplasmic male sterile Brassica juncea.

Plant Sci 2022 Jun 8;319:111244. Epub 2022 Mar 8.

Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China; Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Yazhou District, Sanya 572025, China. Electronic address:

Mitochondrial retrograde signaling (MRS) plays an essential role in sensing and responding to internal and external stimuli to optimize growth to adapt to the prevailing environmental conditions. Previously studies showed alterations on MRS in cytoplasmic male sterile (CMS) plant. However, the regulators involved in MRS in CMS plants remain largely unknown. Read More

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The PV2 cluster of parvalbumin neurons in the murine periaqueductal gray: connections and gene expression.

Brain Struct Funct 2022 Apr 29. Epub 2022 Apr 29.

Anatomy and Program in Neuroscience, Faculty of Science and Medicine, University of Fribourg, CH-1700, Fribourg, Switzerland.

The PV2 (Celio 1990), a cluster of parvalbumin-positive neurons located in the ventromedial region of the distal periaqueductal gray (PAG) has not been previously described as its own entity, leading us to study its extent, connections, and gene expression. It is an oval, bilateral, elongated cluster composed of approximately 475 parvalbumin-expressing neurons in a single mouse hemisphere. In its anterior portion it impinges upon the paratrochlear nucleus (Par4) and in its distal portion it is harbored in the posterodorsal raphe nucleus (PDR). Read More

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Essential Bromodomain BDF2 as a Drug Target against Chagas Disease.

ACS Infect Dis 2022 May 28;8(5):1062-1074. Epub 2022 Apr 28.

Instituto de Biología Molecular y Celular de Rosario, CONICET, 2000 Rosario, Santa Fe, Argentina.

is a unicellular parasite that causes Chagas disease, which is endemic in the American continent but also worldwide, distributed by migratory movements. A striking feature of trypanosomatids is the polycistronic transcription associated with post-transcriptional mechanisms that regulate the levels of translatable mRNA. In this context, epigenetic regulatory mechanisms have been revealed to be of great importance, since they are the only ones that would control the access of RNA polymerases to chromatin. Read More

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Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera).

BMC Plant Biol 2022 Apr 28;22(1):217. Epub 2022 Apr 28.

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities.

Result: In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. Read More

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Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development.

BMC Plant Biol 2022 Apr 28;22(1):219. Epub 2022 Apr 28.

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.

Background: Mentha canadensis L. has important economic value for the production of essential oils, which are synthesised, secreted and stored in peltate glandular trichomes. As a typical multicellular secretory trichome, glandular trichomes are important biological factories for the synthesis of some specialised metabolites. Read More

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Large-scale mapping of positional changes of hypoxia-responsive genes upon activation.

Mol Biol Cell 2022 Apr 27:mbcE21110593. Epub 2022 Apr 27.

Cell Biology of Genomes Group, Center for Cancer Research, National Cancer Institute NIH, Bethesda, 20892.

Chromosome structure and nuclear organization are important factors in the regulation of gene expression. Transcription of a gene is influenced by local and global chromosome features such as chromatin condensation status. The relationship between the 3D position of a gene in the nucleus and its activity is less clear. Read More

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Accurate and highly interpretable prediction of gene expression from histone modifications.

BMC Bioinformatics 2022 Apr 26;23(1):151. Epub 2022 Apr 26.

Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.

Background: Histone Mark Modifications (HMs) are crucial actors in gene regulation, as they actively remodel chromatin to modulate transcriptional activity: aberrant combinatorial patterns of HMs have been connected with several diseases, including cancer. HMs are, however, reversible modifications: understanding their role in disease would allow the design of 'epigenetic drugs' for specific, non-invasive treatments. Standard statistical techniques were not entirely successful in extracting representative features from raw HM signals over gene locations. Read More

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Circadian clock, diurnal glucose metabolic rhythm, and dawn phenomenon.

Trends Neurosci 2022 Jun 21;45(6):471-482. Epub 2022 Apr 21.

Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, Baylor College of Medicine, Houston, TX, USA; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA. Electronic address:

The circadian clock provides cue-independent anticipatory signals for diurnal rhythms of baseline glucose levels and glucose tolerance. The central circadian clock is located in the hypothalamic suprachiasmatic nucleus (SCN), which comprises primarily GABAergic neurons. The SCN clock regulates physiological diurnal rhythms of endogenous glucose production (EGP) and hepatic insulin sensitivity through neurohumoral mechanisms. Read More

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Diagnosis of Subcortical Ischemic Vascular Cognitive Impairment With No Dementia Using Radiomics of Cerebral Cortex and Subcortical Nuclei in High-Resolution T1-Weighted MR Imaging.

Front Oncol 2022 8;12:852726. Epub 2022 Apr 8.

Department of Radiology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: To investigate whether the combination of radiomics derived from brain high-resolution T1-weighted imaging and automatic machine learning could diagnose subcortical ischemic vascular cognitive impairment with no dementia (SIVCIND) accurately.

Methods: A total of 116 right-handed participants involving 40 SIVCIND patients and 76 gender-, age-, and educational experience-matched normal controls (NM) were recruited. A total of 7,106 quantitative features from the bilateral thalamus, hippocampus, globus pallidus, amygdala, nucleus accumbens, putamen, caudate nucleus, and 148 areas of the cerebral cortex were automatically calculated from each subject. Read More

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Comparative Analysis of Transposable Elements and the Identification of Candidate Centromeric Elements in the Subgenus and Its Relatives.

Genes (Basel) 2022 04 2;13(4). Epub 2022 Apr 2.

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

The subgenus and its relatives include many crucial economic drupe fruits and ornamental plants. Repetitive elements make up a large part of complex genomes, and some of them play an important role in gene regulation that can affect phenotypic variation. However, the variation in their genomes remains poorly understood. Read More

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