103 results match your criteria localization incenp


Goldberg-Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis.

J Cell Sci 2021 Jun 8;134(11). Epub 2021 Jun 8.

Neuroscience Institute Cavalieri Ottolenghi, Turin 10123, Italy.

Goldberg-Shprintzen disease (GOSHS) is a rare microcephaly syndrome accompanied by intellectual disability, dysmorphic facial features, peripheral neuropathy and Hirschsprung disease. It is associated with recessive mutations in the gene encoding kinesin family member 1-binding protein (KIF1BP, also known as KIFBP). The encoded protein regulates axon microtubules dynamics, kinesin attachment and mitochondrial biogenesis, but it is not clear how its loss could lead to microcephaly. Read More

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Goldberg-Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis.

J Cell Sci 2021 May 20. Epub 2021 May 20.

Neuroscience Institute Cavalieri Ottolenghi, Turin, Italy.

Goldberg-Shprintzen disease (GOSHS) is a rare microcephaly syndrome accompanied by intellectual disability, dysmorphic facial features, peripheral neuropathy and Hirschsprung disease. It is associated with recessive mutations in the gene encoding kinesin family member 1-binding protein (KIF1BP). The encoded protein regulates axon microtubules dynamics, kinesin attachment and mitochondrial biogenesis, but it is not clear how its loss could lead to microcephaly. Read More

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An ATM-CHK2-INCENP pathway prevents chromatin breakage by regulating the abscission checkpoint.

Mol Cell Oncol 2021 8;8(2):1877999. Epub 2021 Feb 8.

Department of Biology, University of Crete, Heraklion, Greece.

In response to chromatin bridges, the chromosomal passenger complex (CPC) delays completion of cytokinesis (abscission) to prevent chromosome breakage. Here, we discuss recent findings from our lab showing that an ATM-CHK2-INCENP pathway imposes the abscission checkpoint in human cells by regulating CPC midbody-localization. Read More

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February 2021

Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules.

J Cell Biol 2021 Jun;220(6)

Waksman Institute and Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, NJ.

The chromosomes in the oocytes of many animals appear to promote bipolar spindle assembly. In Drosophila oocytes, spindle assembly requires the chromosome passenger complex (CPC), which consists of INCENP, Borealin, Survivin, and Aurora B. To determine what recruits the CPC to the chromosomes and its role in spindle assembly, we developed a strategy to manipulate the function and localization of INCENP, which is critical for recruiting the Aurora B kinase. Read More

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An ATM-Chk2-INCENP pathway activates the abscission checkpoint.

J Cell Biol 2021 Feb;220(2)

Department of Biology, University of Crete, Heraklion, Greece.

During cell division, in response to chromatin bridges, the chromosomal passenger complex (CPC) delays abscission to prevent chromosome breakage or tetraploidization. Here, we show that inhibition of ATM or Chk2 kinases impairs CPC localization to the midbody center, accelerates midbody resolution in normally segregating cells, and correlates with premature abscission and chromatin breakage in cytokinesis with trapped chromatin. In cultured human cells, ATM activates Chk2 at late midbodies. Read More

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February 2021

Functional Analysis of the Plant Chromosomal Passenger Complex.

Plant Physiol 2020 08 27;183(4):1586-1599. Epub 2020 May 27.

University of Hamburg, Institute for Plant Sciences and Microbiology, Department of Developmental Biology, D-22609 Hamburg, Germany

The Aurora B kinase, encoded by the () gene in Arabidopsis (), is a key regulator of cell division in all eukaryotes. Aurora B has at least two central functions during cell division; it is essential for the correct, i.e. Read More

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Molecular basis of MKLP2-dependent Aurora B transport from chromatin to the anaphase central spindle.

J Cell Biol 2020 07;219(7)

Department of Biochemistry, University of Oxford, Oxford, UK.

The Aurora B chromosomal passenger complex (CPC) is a conserved regulator of mitosis. Its functions require localization first to the chromosome arms and then centromeres in mitosis and subsequently the central spindle in anaphase. Here, we analyze the requirements for core CPC subunits, survivin and INCENP, and the mitotic kinesin-like protein 2 (MKLP2) in targeting to these distinct localizations. Read More

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Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.

J Cell Biol 2020 03;219(3)

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO.

Aurora B kinase has a critical role in regulating attachments between kinetochores and spindle microtubules during mitosis. Early in mitosis, kinase activity at kinetochores is high to promote attachment turnover, and in later mitosis, activity decreases to ensure attachment stabilization. Aurora B localizes prominently to inner centromeres, and a population of the kinase is also detected at kinetochores. Read More

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Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions.

Cell Cycle 2019 09 15;18(17):2006-2025. Epub 2019 Jul 15.

a Sars International Centre for Marine Molecular Biology, University of Bergen , Bergen , Norway.

A single inner centromere protein (INCENP) found throughout eukaryotes modulates Aurora B kinase activity and chromosomal passenger complex (CPC) localization, which is essential for timely mitotic progression. It has been proposed that INCENP might act as a rheostat to regulate Aurora B activity through mitosis, with successively higher activity threshold levels for chromosome alignment, the spindle checkpoint, anaphase spindle transfer and finally spindle elongation and cytokinesis. It remains mechanistically unclear how this would be achieved. Read More

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September 2019

Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast.

Curr Biol 2019 05 18;29(9):1536-1544.e4. Epub 2019 Apr 18.

Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK. Electronic address:

For proper chromosome segregation in mitosis, sister kinetochores must interact with microtubules from opposite spindle poles (chromosome bi-orientation) [1, 2]. To promote bi-orientation, Aurora B kinase disrupts aberrant kinetochore-microtubule interactions [3-6]. It has long been debated how Aurora B halts this action when bi-orientation is established and tension is applied across sister kinetochores. Read More

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Point centromere activity requires an optimal level of centromeric noncoding RNA.

Proc Natl Acad Sci U S A 2019 03 8;116(13):6270-6279. Epub 2019 Mar 8.

School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong

In budding yeast, which possesses simple point centromeres, we discovered that all of its centromeres express long noncoding RNAs (cenRNAs), especially in S phase. Induction of cenRNAs coincides with CENP-A loading time and is dependent on DNA replication. Centromeric transcription is repressed by centromere-binding factor Cbf1 and histone H2A variant H2A. Read More

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Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion.

J Biol Chem 2019 02 6;294(6):2021-2035. Epub 2018 Dec 6.

From the Ministry of Education (MOE) Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China

The chromosomal passenger complex (CPC) is a master regulator of mitosis. CPC consists of inner centromere protein (INCENP), Survivin, Borealin, and the kinase Aurora B and plays key roles in regulating kinetochore-microtubule attachments and spindle assembly checkpoint signaling. However, the role of CPC in sister chromatid cohesion, mediated by the cohesin complex, remains incompletely understood. Read More

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February 2019

Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle: a prerequisite for productive endodyogeny.

Cell Mol Life Sci 2018 Dec 26;75(23):4417-4443. Epub 2018 Jul 26.

Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, INSERM, Université de Montpellier, Montpellier, France.

The phylum Apicomplexa encompasses deadly pathogens such as malaria and Cryptosporidium. Apicomplexa cell division is mechanistically divergent from that of their mammalian host, potentially representing an attractive source of drug targets. Depending on the species, apicomplexan parasites can modulate the output of cell division, producing two to thousands of daughter cells at once. Read More

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December 2018

Kinesin 6 Regulation in Female Meiosis by the Non-conserved N- and C- Terminal Domains.

G3 (Bethesda) 2018 05 4;8(5):1555-1569. Epub 2018 May 4.

Waksman Institute, Rutgers, the State University of New Jersey, NJ-08854

Bipolar spindle assembly occurs in the absence of centrosomes in the oocytes of most organisms. In the absence of centrosomes in oocytes, we have proposed that the kinesin 6 Subito, a MKLP-2 homolog, is required for establishing spindle bipolarity and chromosome biorientation by assembling a robust central spindle during prometaphase I. Although the functions of the conserved motor domains of kinesins is well studied, less is known about the contribution of the poorly conserved N- and C- terminal domains to motor function. Read More

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Haspin inhibition reveals functional differences of interchromatid axis-localized AURKB and AURKC.

Mol Biol Cell 2017 Aug 28;28(17):2233-2240. Epub 2017 Jun 28.

Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854

Aneuploidy is the leading genetic abnormality contributing to infertility, and chromosome segregation errors are common during female mammalian meiosis I (MI). Previous results indicate that haspin kinase regulates resumption of meiosis from prophase arrest, chromosome condensation, and kinetochore-microtubule attachments during early prometaphase of MI. Here we report that haspin inhibition in late prometaphase I causes acceleration of MI, bypass of the spindle assembly checkpoint (SAC), and loss of interchromatid axis-localized Aurora kinase C. Read More

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Dual recognition of chromatin and microtubules by INCENP is important for mitotic progression.

J Cell Biol 2017 04 17;216(4):925-941. Epub 2017 Mar 17.

Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065

The chromosomal passenger complex (CPC), composed of inner centromere protein (INCENP), Survivin, Borealin, and the kinase Aurora B, contributes to the activation of the mitotic checkpoint. The regulation of CPC function remains unclear. Here, we reveal that in addition to Survivin and Borealin, the single α-helix (SAH) domain of INCENP supports CPC localization to chromatin and the mitotic checkpoint. Read More

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Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.

Methods Mol Biol 2017 ;1510:47-59

Centre for Medical Biotechnology (ZMB), Institute for Molecular Biology II, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany.

The chromosomal passenger complex (CPC) is a key regulator of cell division. Its proper localization during the different phases of mitosis and cytokinesis is crucial for the exertion of its various functions. HDACi treatment has been demonstrated to disturb the centromeric localization of the CPC in tumor cells, thus leading to severe mitotic defects often followed by apoptosis. Read More

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January 2018

Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex.

PLoS One 2016 22;11(6):e0157305. Epub 2016 Jun 22.

Department of Translational Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, Texas, United States of America.

Aurora-C, a member of the Aurora kinase family that can complement Aurora-B function in mitosis is either moderately expressed or repressed in most adult somatic tissues but is active in early embryonic development and expressed at elevated levels in multiple human cancers. Aurora-C overexpression reportedly plays a role in tumorigenic transformation. We performed detailed characterization of Aurora-C interactions with members of the Chromosome Passenger Complex (CPC), Survivin and Inner Centromere Protein (INCENP) in reference to known Aurora-B interactions to understand the functional significance of Aurora-C overexpression in human cancer cells. Read More

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Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis.

Open Biol 2016 Mar;6(3)

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK

Cytokinesis culminates in the final separation, or abscission, of the two daughter cells at the end of cell division. Abscission relies on an organelle, the midbody, which forms at the intercellular bridge and is composed of various proteins arranged in a precise stereotypic pattern. The molecular mechanisms controlling midbody organization and function, however, are obscure. Read More

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The Inner Centromere Protein (INCENP) Coil Is a Single α-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis.

J Biol Chem 2015 Aug 14;290(35):21460-72. Epub 2015 Jul 14.

From The Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Max Born Crescent, Edinburgh EH9 3BF, Scotland, United Kingdom and

The chromosome passenger complex (CPC) is a master regulator of mitosis. Inner centromere protein (INCENP) acts as a scaffold regulating CPC localization and activity. During early mitosis, the N-terminal region of INCENP forms a three-helix bundle with Survivin and Borealin, directing the CPC to the inner centromere where it plays essential roles in chromosome alignment and the spindle assembly checkpoint. Read More

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Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules.

Cell Rep 2015 Jul 9;12(3):380-7. Epub 2015 Jul 9.

Department of Medical Oncology, Department of Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG Utrecht, the Netherlands. Electronic address:

The chromosomal passenger complex is essential for error-free chromosome segregation and proper execution of cytokinesis. To coordinate nuclear division with cytoplasmic division, its enzymatic subunit, Aurora B, relocalizes from centromeres in metaphase to the spindle midzone in anaphase. In budding yeast, this requires dephosphorylation of the microtubule-binding (MTB) domain of the INCENP analog Sli15. Read More

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Spatial Compartmentalization Specializes the Function of Aurora A and Aurora B.

J Biol Chem 2015 Jul 18;290(28):17546-58. Epub 2015 May 18.

Ministry of Education Key Laboratory of Cell Proliferation and Differentiation and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China

Aurora kinase A and B share great similarity in sequences, structures, and phosphorylation motif, yet they show different localizations and play distinct crucial roles. The factors that determine such differences are largely unknown. Here we targeted Aurora A to the localization of Aurora B and found that Aurora A phosphorylates the substrate of Aurora B and substitutes its function in spindle checkpoint. Read More

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The chromosomal passenger complex (CPC) as a key orchestrator of orderly mitotic exit and cytokinesis.

Front Cell Dev Biol 2015 5;3:14. Epub 2015 Mar 5.

Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School Singapore Singapore.

Understanding the molecular network of orderly mitotic exit to re-establish a functional interphase nucleus is critical because disordered mitotic exit inevitably leads to genomic instability. In contrast to the mechanisms of the entrance to mitosis, however, little is known about what controls the orderly exit from mitosis, particularly in mammalian cells. The chromosomal passenger complex (CPC), which is composed of Aurora B, INCENP, Borealin and Survivin, is one of the most widely studied and highly conserved hetero-tetrameric complexes. Read More

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Disruption of a conserved CAP-D3 threonine alters condensin loading on mitotic chromosomes leading to chromosome hypercondensation.

J Biol Chem 2015 Mar 20;290(10):6156-67. Epub 2015 Jan 20.

From the Murdoch Childrens Research Institute, Royal Children's Hospital, Melbourne, Victoria 3052 and the Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Melbourne, Victoria 3052, Australia

The condensin complex plays a key role in organizing mitotic chromosomes. In vertebrates, there are two condensin complexes that have independent and cooperative roles in folding mitotic chromosomes. In this study, we dissect the role of a putative Cdk1 site on the condensin II subunit CAP-D3 in chicken DT40 cells. Read More

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Targeting the INCENP IN-box-Aurora B interaction to inhibit CPC activity in vivo.

Open Biol 2014 Nov;4(11):140163

Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Michael Swann Building, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, UK

The chromosome passenger complex (CPC) is an essential regulator of mitosis and cytokinesis. The CPC consists of Aurora B kinase, inner centromere protein (INCENP), and the targeting subunits survivin and borealin/Dasra B. INCENP is a scaffolding subunit for the CPC and activates Aurora B via its conserved IN-box domain. Read More

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November 2014

Polo kinase regulates the localization and activity of the chromosomal passenger complex in meiosis and mitosis in Drosophila melanogaster.

Open Biol 2014 Nov;4(11):140162

The Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK

Cell cycle progression is regulated by members of the cyclin-dependent kinase (CDK), Polo and Aurora families of protein kinases. The levels of expression and localization of the key regulatory kinases are themselves subject to very tight control. There is increasing evidence that crosstalk between the mitotic kinases provides for an additional level of regulation. Read More

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November 2014

Chromatin protein HP1 interacts with the mitotic regulator borealin protein and specifies the centromere localization of the chromosomal passenger complex.

J Biol Chem 2014 Jul;289(30):20638-49

Accurate mitosis requires the chromosomal passenger protein complex (CPC) containing Aurora B kinase, borealin, INCENP, and survivin, which orchestrates chromosome dynamics. However, the chromatin factors that specify the CPC to the centromere remain elusive. Here we show that borealin interacts directly with heterochromatin protein 1 (HP1) and that this interaction is mediated by an evolutionarily conserved PXVXL motif in the C-terminal borealin with the chromo shadow domain of HP1. Read More

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Involvement of satellite I noncoding RNA in regulation of chromosome segregation.

Genes Cells 2014 Jun 21;19(6):528-38. Epub 2014 Apr 21.

Department of Biological Sciences, Graduate School of Science Technology, Kumamoto University, Kumamoto, 860-8555, Japan.

Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Read More

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Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B.

Cell Death Dis 2014 Mar 6;5:e1106. Epub 2014 Mar 6.

1] Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan [2] Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan [3] Infectious Disease and Signaling Research Center, National Cheng Kung University, Tainan, Taiwan [4] Institute for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.

The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Read More

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Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiae.

PLoS One 2014 18;9(2):e89399. Epub 2014 Feb 18.

Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Scotland, United Kingdom.

The chromosomal passenger complex (CPC) is a key regulator of eukaryotic cell division, consisting of the protein kinase Aurora B/Ipl1 in association with its activator (INCENP/Sli15) and two additional proteins (Survivin/Bir1 and Borealin/Nbl1). Here we have identified multiple sites of CPC autophosphorylation on yeast Sli15 that are located within its central microtubule-binding domain and examined the functional significance of their phosphorylation by Ipl1 through mutation of these sites, either to non-phosphorylatable alanine (sli15-20A) or to acidic residues to mimic constitutive phosphorylation (sli15-20D). Both mutant sli15 alleles confer chromosome instability, but this is mediated neither by changes in the capacity of Sli15 to activate Ipl1 kinase nor by decreased efficiency of chromosome biorientation, a key process in cell division that requires CPC function. Read More

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October 2014