640 results match your criteria living transparent


Engaging Sexual and Gender Minority Youth in HIV Interventions Through Gay Dating Apps: Recruitment Protocol.

JMIR Res Protoc 2021 06 22;10(6):e28864. Epub 2021 Jun 22.

Department of Pediatrics, School of Medicine, Tulane University, New Orleans, LA, United States.

Background: HIV continues to disproportionately impact sexual and gender minority youth (SGMY) in the United States. Public health efforts have increasingly focused on developing efficacious interventions to curb the spread of HIV among SGMY and help those living with HIV achieve and sustain viral suppression. However, recruiting and engaging SGMY in prevention and care interventions is challenging. Read More

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Pupil-modulation ghost phase imaging.

Appl Opt 2021 May;60(15):4398-4403

Computational ghost imaging (CGI) allows us to reconstruct images under a low signal-to-noise-ratio condition. However, CGI cannot retrieve phase information; it is unsuitable for observation of transparent objects such as living cells. A phase imaging method with CGI architecture is proposed. Read More

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An in vivo reporter for tracking lipid droplet dynamics in transparent zebrafish.

Elife 2021 Jun 11;10. Epub 2021 Jun 11.

Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, United States.

Lipid droplets are lipid storage organelles found in nearly all cell types from adipocytes to cancer cells. Although increasingly implicated in disease, current methods to study lipid droplets in vertebrate models rely on static imaging or the use of fluorescent dyes, limiting investigation of their rapid in vivo dynamics. To address this, we created a lipid droplet transgenic reporter in whole animals and cell culture by fusing tdTOMATO to Perilipin-2 (PLIN2), a lipid droplet structural protein. Read More

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Occurrence and characterization of microplastics in white shrimp, Metapenaeus affinis, living in a habitat highly affected by anthropogenic pressures, northwest Persian Gulf.

Mar Pollut Bull 2021 Jun 7;169:112581. Epub 2021 Jun 7.

Shrimp Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bushehr 75169-89177, Iran. Electronic address:

Microplastics (MPs) are a modern societal concern and recognized as a growing environmental hazard by careless disposal. This study aimed to assess the MPs content in white shrimp (Metapenaeus affinis) inhabiting in a natural habitat affected by high anthropogenic pressures, and recognize if the shrimp could be a suitable bioindicator for MPs pollution. To assess spatial changes of MPs presence in shrimps, sampling was carried out by a trawl net from 13 stations across the entire Musa Bay. Read More

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Epidemiology and burden of progressive familial intrahepatic cholestasis: a systematic review.

Orphanet J Rare Dis 2021 Jun 3;16(1):255. Epub 2021 Jun 3.

, Roboleo & Co, Leeds, UK.

Background: Progressive familial intrahepatic cholestasis is a rare, heterogeneous group of liver disorders of autosomal recessive inheritance, characterised by an early onset of cholestasis with pruritus and malabsorption, which rapidly progresses, eventually culminating in liver failure. For children and their parents, PFIC is an extremely distressing disease. Significant pruritus can lead to severe cutaneous mutilation and may affect many activities of daily living through loss of sleep, irritability, poor attention, and impaired school performance. Read More

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Morphology and sexual dimorphism of living mature adults of (Marenzeller, 1875) (Annelida, Hesionidae, Amphidurine), first reported for the Iberian Peninsula.

Biodivers Data J 2021 19;9:e66020. Epub 2021 May 19.

Centre d'Estudis Avancats de Blanes (CEAB - CSIC), Blanes, Spain Centre d'Estudis Avancats de Blanes (CEAB - CSIC) Blanes Spain.

Background: To date, the genus (Annelida: Hesionidae: Amphidurine) is considered as monotypic. Its single species, (Marenzeller, 1875), is well characterised by lacking proboscideal papillae and emerging acicular chaetae, as well as by having three antennae, eight pairs of tentacular cirri and inflated dorsal cirri with characteristic alternating length and colour (transparent, with median orange band and white tips) in live animals.

New Information: Three specimens, one male and two females, were found below boulders at 5-7 m depth in Punta Santa Anna, Blanes and Cala Maset, Sant Feliu de Guixols (Catalan Sea, NW Mediterranean, Iberian Peninsula). Read More

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New perspectives on the calculation of bioaccumulation metrics for active substances in living organisms.

Integr Environ Assess Manag 2021 May 12. Epub 2021 May 12.

Laboratoire de Biométrie et Biologie Evolutive, Université de Lyon, Villeurbanne, France.

Today, only few ready-to-use and convenient decision-making tools are available in ecotoxicology concerning accumulation and effects of chemical substances on organisms, accounting for exposure situations that are known to be complex (routes of exposure, metabolism, mixtures, etc.). This paper presents new perspectives on the generic calculation of bioaccumulation metrics via the innovative web tool MOSAIC (http://mosaic. Read More

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Predicting falls in community-dwelling older adults: a systematic review of prognostic models.

BMJ Open 2021 05 4;11(5):e044170. Epub 2021 May 4.

Department of Geriatric Medicine, Aalborg University Hospital, Aalborg, Denmark.

Objective: To systematically review and critically appraise prognostic models for falls in community-dwelling older adults.

Eligibility Criteria: Prospective cohort studies with any follow-up period. Studies had to develop or validate multifactorial prognostic models for falls in community-dwelling older adults (60+ years). Read More

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Diffusion of LLPS Droplets Consisting of Poly(PR) Dipeptide Repeats and RNA on Chemically Modified Glass Surface.

Langmuir 2021 05 30;37(18):5635-5641. Epub 2021 Apr 30.

Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.

The liquid-liquid phase separation (LLPS) of proteins and RNA molecules has emerged in recent years as an important physicochemical process to explain the organization of membrane-less organelles in living cells and cellular functions and even some fatal neurodegenerative diseases, such as Amyotrophic Lateral Sclerosis (ALS) due to the spontaneous condensation and growth of LLPS droplets. In general, the characterization of LLPS droplets has been performed by optical microscopy, where we need transparent substrates. By virtue of the liquid and wetting properties of LLPS droplets on a glass surface, there have been some technical protocols recommended to immobilize droplets on the surfaces. Read More

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Stretchable and Transparent Electrochemical Sensor Based on Nanostructured Au on Carbon Nanotube Networks for Real-Time Analysis of HO Release from Cells.

Anal Chem 2021 05 23;93(17):6723-6730. Epub 2021 Apr 23.

School of Public Health, Nantong University, Nantong 226019, P. R. China.

Various electrochemical biosensors have been developed for direct and real-time recording of biomolecules released from living cells. However, since these traditional electrodes are commonly rigid and nonflexible, in situ monitoring of biochemical signals while cell deformation occurs remains a great challenge. Herein, we report a facile approach for the development of a stretchable and transparent electrochemical cell-sensing platform based on Au nanostructures (nano-Au) and carbon nanotube (CNT) films embedded in PDMS (nano-Au/CNTs/PDMS). Read More

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Monitoring Anomalies in 3D Bioprinting with Deep Neural Networks.

ACS Biomater Sci Eng 2021 Apr 21. Epub 2021 Apr 21.

Department of Mechanical Engineering, University of California, Berkeley, California 94720, United States.

Additive manufacturing technologies have progressed in the past decades, especially when used to print biofunctional structures such as scaffolds and vessels with living cells for tissue engineering applications. Part quality and reliability are essential to maintaining the biocompatibility and structural integrity needed for engineered tissue constructs. As a result, it is critical to detect for any anomalies that may occur in the 3D-bioprinting process that can cause a mismatch between the desired designs and printed shapes. Read More

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Optically transparent electrodes to study living cells: A mini review.

Biotechnol Bioeng 2021 Jul 23;118(7):2393-2400. Epub 2021 Apr 23.

Intensive Care Unit, N. V. Sklifosovsky Research Institute for Emergency Medicine, Moscow, Russia.

The use of electrochemical methods to study living systems, including cells, has been of interest to researchers for a long time. Thus, controlling the polarization of the electrode contacting living cells, one can influence, for example, their proliferation or the synthesis of specific proteins. Moreover, the electrochemical approach formed the basis of the biocompatibility improvement of the materials contacting with body tissues that use in carbon hemosorbents and implants development. Read More

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Observation of Cells on a Simulated Titanium Surface with Transparency.

J Dent Res 2021 Mar 23:220345211000272. Epub 2021 Mar 23.

National Institute of Advanced Industrial Science and Technology, GaN Advanced Device Open Innovation Laboratory, Nagoya, Aichi, Japan.

The main driving force of osseointegration on titanium implants is believed to be the calcification caused by cellular activity. However, owing to the opacity of bulk titanium, live cells on titanium surfaces cannot be observed using an inverted microscope. To overcome this limitation, this study proposes a transparent titanium thin layer as a simulated titanium surface that allows live-cell observation from below. Read More

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Marine Debris and Trace Metal (Cu, Cd, Pb, and Zn) Pollution in the Stranded Green Sea Turtles (Chelonia mydas).

Arch Environ Contam Toxicol 2021 Apr 22;80(3):634-644. Epub 2021 Mar 22.

Department of Fisheries, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Province of Sistan dan Baluchestan, Iran.

Marine debris and trace metals are among the common environmental contaminants known to affect marine organisms. In this study, the quantitative and qualitative aspects of marine debris levels and bioaccumulation of trace metals (Cadmium: Cd; Copper: Cu; Lead: Pb; and Zinc: Zn) were investigated in 42 green sea turtles (Chelonia mydas) stranded on the northern coast of the Sea of Oman. The greatest quantity of debris was found in the intestine, stomach, and esophagus of the animals, respectively. Read More

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Toward improved homecare of frail older adults: A focus group study synthesizing patient and caregiver perspectives.

Aging Med (Milton) 2021 Mar 21;4(1):4-11. Epub 2021 Jan 21.

Health Sciences and Innovation Surrey Memorial Hospital Fraser Health Surrey British Columbia Canada.

Background: Adopting a better understanding of how both older adults and health care providers view the community management of frailty is necessary for improving home health, especially facing the coronavirus disease 2019 (COVID-19) pandemic. We conducted a qualitative focus group study to assess how both older adults and health care providers view frailty and virtual health care in home health.

Methods: Two focus groups enrolled home-living older adults and health care professionals, respectively (n = 15). Read More

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Robust Hybrid Omniphobic Surface for Stain Resistance.

ACS Appl Mater Interfaces 2021 Mar 4;13(12):14562-14568. Epub 2021 Mar 4.

State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, PR China.

Inspired by natural living surfaces, researchers have developed many excellent anti-smudge coatings, but there remain some critical challenges such as complex or expensive fabrications, poor long-term stability, non-transparency, etc., which may limit their large-area application. In this work, we designed a robust and transparent omniphobic coating with a one-step dip coating method. Read More

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Live-cell Imaging and Quantitative Analysis of Meiotic Divisions in Males.

Bio Protoc 2020 Oct 20;10(20):e3785. Epub 2020 Oct 20.

Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.

Live-imaging of meiotic cell division has been performed in extracted spermatocytes of a number of species using phase-contrast microscopy. For the nematode , removal of spermatocytes from gonads has damaging effects, as most of the extracted spermatocytes show a high variability in the timing of meiotic divisions or simply arrest during the experiment. Therefore, we developed a live-cell imaging approach for filming of spermatocyte meiosis in whole immobilized males, thus allowing an observation of male germ cells within an unperturbed environment. Read More

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October 2020

Open Science and Intervention Research: a Program Developer's and Researcher's Perspective on Issues and Concerns.

Authors:
John E Lochman

Prev Sci 2021 Mar 2. Epub 2021 Mar 2.

The University of Alabama, Tuscaloosa, AL, USA.

Open Science practices bear great promise for making research in general more reproducible and transparent, and these goals are very important for preventive intervention research. From my perspective as a program co-developer, I note potential concerns and issues of how open science practices can be used in intervention research. Key issues considered are in the realms of pre-registration (making pre-registration a living document; providing rewards for hypothesis-generating research, in addition to hypothesis-testing research), data archiving (resources for data archiving of large datasets; ethical issues related to need for strong de-identification), and research materials (intervention manuals and materials, and characteristics, training and supervision of intervention staff). Read More

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Birefringent Silk Fibroin Hydrogel Constructed via Binary Solvent-Exchange-Induced Self-Assembly.

Biomacromolecules 2021 May 1;22(5):1955-1965. Epub 2021 Mar 1.

School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, China.

Birefringent hydrogels have a strong potential for applications in biomedicine and optics as they can modulate the optical and mechanical anisotropy in confined two-dimensional geometries. However, production of birefringent hydrogels with hierarchical structures, mechanical properties, and biorelated behavior that are analogous to biological tissues is still challenging. Starting from the silk fibroin (SF)-ionic liquid solution system, this study aimed to rationally design a "binary solvent-exchange-induced self-assembly (BSEISA)" strategy to produce birefringent SF hydrogels (SFHs). Read More

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Innovative fluorescent probes for in vivo visualization of biomolecules in living Caenorhabditis elegans.

Cytometry A 2021 Jun 27;99(6):560-574. Epub 2021 Feb 27.

Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China.

Caenorhabditis elegans (C. elegans) as a well-established multicellular model organism has been widely used in the biological field for half a century. Its numerous advantages including small body size, rapid life cycle, high-reproductive rate, well-defined anatomy, and conserved genome, has made C. Read More

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Rolled-Up Metal Oxide Microscaffolds to Study Early Bone Formation at Single Cell Resolution.

Small 2021 Mar 18;17(12):e2005527. Epub 2021 Feb 18.

Institute for Integrative Nanosciences, Leibniz IFW Dresden e.V., Helmholtzstraße 20, Dresden, 01069, Germany.

Titanium and its alloys are frequently used to replace structural components of the human body due to their high mechanical strength, low stiffness, and biocompatibility. In particular, the use of porous materials has improved implant stabilization and the promotion of bone. However, it remains unclear which material properties and geometrical cues are optimal for a proper osteoinduction and osseointegration. Read More

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Normalising life at risk of Huntington's disease. A qualitative study of backgrounds and coping strategies of fears of genetic discrimination.

Eur J Hum Genet 2021 Jun 16;29(6):940-948. Epub 2021 Feb 16.

Life Sciences & Society Lab, Centre for Sociological Research, KU Leuven, 45 - 3601, Parkstraat, Leuven, 3000, Belgium.

Studies in the sociology of genetics have shown how living with a predisposition to a genetic disorder often comes with significant psycho-social burdens and struggles. One of these struggles is the fear of genetic discrimination. Despite genetic non-discrimination regulations, research shows people still worry about being subjected to genetic discrimination. Read More

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Fostering Engagement With Health and Housing Innovation: Development of Participant Personas in a Social Housing Cohort.

JMIR Public Health Surveill 2021 02 16;7(2):e25037. Epub 2021 Feb 16.

European Centre for Environment and Human Health, University of Exeter, Truro, Cornwall, United Kingdom.

Background: Personas, based on customer or population data, are widely used to inform design decisions in the commercial sector. The variety of methods available means that personas can be produced from projects of different types and scale.

Objective: This study aims to experiment with the use of personas that bring together data from a survey, household air measurements and electricity usage sensors, and an interview within a research and innovation project, with the aim of supporting eHealth and eWell-being product, process, and service development through broadening the engagement with and understanding of the data about the local community. Read More

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February 2021

The risk from SARS-CoV-2 to bat species in england and mitigation options for conservation field workers.

Transbound Emerg Dis 2021 Feb 11. Epub 2021 Feb 11.

Institute of Zoology, Zoological Society of London, London, UK.

The newly evolved coronavirus, SARS-CoV-2, which has precipitated a global COVID-19 pandemic among the human population, has been shown to be associated with disease in captive wild animals. Bats (Chiroptera) have been shown to be susceptible to experimental infection and therefore may be at risk from disease when in contact with infected people. Numerous conservation fieldwork activities are undertaken across the United Kingdom bringing potentially infected people into close proximity with bats. Read More

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February 2021

A mega-aggregation framework synthesis of the barriers and facilitators to linkage, adherence to ART and retention in care among people living with HIV.

Syst Rev 2021 02 11;10(1):54. Epub 2021 Feb 11.

Centre for Evidence-Based Health Care, Division Epidemiology and Biostatistics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Background: People living with human immunodeficiency virus (PLHIV) struggle with the challenges of living with a chronic disease and integrating antiretroviral treatment (ART) and care into their daily lives. The aims of this study were as follows: (1) to undertake the first mega-aggregation of qualitative evidence syntheses using the methods of framework synthesis and (2) make sense of existing qualitative evidence syntheses that explore the barriers and facilitators of adherence to antiretroviral treatment, linkage to care and retention in care for PLHIV to identify research gaps.

Methods: We conducted a comprehensive search and did all screening, data extraction and critical appraisal independently and in duplicate. Read More

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February 2021

3D-Printed Biomimetic Systems with Synergetic Color and Shape Responses Based on Oblate Cholesteric Liquid Crystal Droplets.

Adv Mater 2021 Mar 1;33(10):e2006361. Epub 2021 Feb 1.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Living organisms in nature have amazing control over their color, shape, and morphology in response to environmental stimuli for camouflage, communication, or reproduction. Inspired by the camouflage of the octopus via the elongation or contraction of its pigment cells, oblate cholesteric liquid crystal droplets are dispersed in a polymer matrix, serving as the role of pigment cells and showing structural color due to selective Bragg reflection by their periodic helical structure. The color of 3D-printed biomimetic systems can be tuned by changing the helical pitch via the chiral dopant concentration or temperature. Read More

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Potential of blockchain approach on development and security of microbial databases.

Biol Proced Online 2021 Feb 1;23(1). Epub 2021 Feb 1.

Department of Computer Science, School of Mathematics, Statistics and Computer Science, College of Science, University of Tehran, Tehran, 14155-6455, Iran.

Approaches developed based on the blockchain concept can provides a framework for the realization of open science. The traditional centralized way of data collection and curation is a labor-intensive work that is often not updated. The fundamental contribution of developing blockchain format of microbial databases includes: 1. Read More

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February 2021

Early Perceptions of COVID-19 Contact Tracing Apps in German-Speaking Countries: Comparative Mixed Methods Study.

J Med Internet Res 2021 02 8;23(2):e25525. Epub 2021 Feb 8.

Institute of History and Ethics in Medicine, Technical University Munich, Munich, Germany.

Background: The main German-speaking countries (Germany, Austria, and Switzerland) have implemented digital contact tracing apps to assist the authorities with COVID-19 containment strategies. Low user rates for these apps can affect contact tracing and, thus, its usefulness in controlling the spread of the novel coronavirus.

Objective: This study aimed to assess the early perceptions of people living in the German-speaking countries and compare them with the frames portrayed in the newspapers during the first wave of the COVID-19 pandemic. Read More

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February 2021

Recent Advances on Bioprinted Gelatin Methacrylate-Based Hydrogels for Tissue Repair.

Tissue Eng Part A 2021 Jun 9;27(11-12):679-702. Epub 2021 Mar 9.

Department of Mechanical and Industrial Engineering, Norwegian University of Science and Technology, Trondheim, Norway.

Bioprinting of body tissues has gained great attention in recent years due to its unique advantages, including the creation of complex geometries and printing the patient-specific tissues with various drug and cell types. The most momentous part of the bioprinting process is bioink, defined as a mixture of living cells and biomaterials (especially hydrogels). Among different biomaterials, natural polymers are the best choices for hydrogel-based bioinks due to their intrinsic biocompatibility and minimal inflammatory response in body condition. Read More

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A guide to representing variability and uncertainty in biodiversity indicators.

Conserv Biol 2021 Jan 24. Epub 2021 Jan 24.

Centre of Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, 3216, Australia.

Biodiversity indicators are used to inform decisions and measure progress toward global targets, such as the United Nations Sustainable Development Goals. Indicators aggregate and simplify complex information, so underlying information influencing its reliability and interpretation (e.g. Read More

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January 2021