21,492 results match your criteria liver donors

Virtual noncontrast images derived from dual-energy CT for assessment of hepatic steatosis in living liver donors.

Eur J Radiol 2021 Mar 27;139:109687. Epub 2021 Mar 27.

Department of Radiology, Seoul National University Hospital, Seoul, South Korea; Department of Radiology, Seoul National University College of Medicine, Seoul, South Korea; Institute of Radiation Medicine, Seoul National University Medical Research Center, Seoul, South Korea.

Purpose: This study aimed to investigate the correlation of attenuation between virtual noncontrast (VNC) and true noncontrast (TNC) CT images and compare the diagnostic performance for hepatic steatosis using MR spectroscopy (MRS) as the reference standard.

Methods: A total of 131 consecutive hepatic donor candidates who underwent dual-source dual-energy CT and MRS within one month from January 2018 to April 2019 were included. An MRS value > 5. Read More

View Article and Full-Text PDF

Generation of Murine Bone Marrow and Fetal Liver Chimeras.

Curr Protoc 2021 Apr;1(4):e79

Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia.

The generation of radiation chimeras allows researchers to substitute the hematopoietic system of a mouse with that of one or more donors. A suspension of hematopoietic stem cells (HSCs) is prepared from the bone marrow (BM) or the fetal liver (FL) of a donor mouse and adoptively transferred into an irradiated recipient. Within days, the donor's HSCs will engraft, and their progeny will quickly replace the blood cells of the recipient. Read More

View Article and Full-Text PDF

The role of ex-situ hypothermic oxygenated machine perfusion and cold preservation time in extended criteria DCD and DBD.

Liver Transpl 2021 Apr 9. Epub 2021 Apr 9.

General and Liver Transplant Surgery Unit - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Milan.

Hypothermic-oxygenated machine perfusion (HOPE) has the potential to counterbalance the detrimental consequences of cold and warm ischemia time in both brain death donors (DBD) and donors after circulatory death (DCD). Herein, we investigated the protective effects of HOPE in extended criteria (ECD) DBD and over-extended warm ischemia time (WIT) DCD grafts. The present retrospective case-series study included 50 livers subjected to end-ischemic HOPE or dual (D)HOPE in two liver transplant (LT) Centers from January 2018 to December 2019. Read More

View Article and Full-Text PDF

Reduction of Visceral Adiposity as a Predictor for Resolution of Nonalcoholic Fatty Liver in Potential Living Liver Donors.

Liver Transpl 2021 Apr 9. Epub 2021 Apr 9.

Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

This study aimed to determine the factors associated with resolution of nonalcoholic fatty liver (NAFL) after lifestyle intervention in potential living liver donors, as assessed by gold standards in a longitudinal setting. This retrospective study included 115 potential living liver donors (mean age, 30.5 ± 7. Read More

View Article and Full-Text PDF

Co-expression of drug metabolizing cytochrome P450 enzymes and estrogen receptor alpha (ESR1) in human liver: racial differences and the regulatory role of ESR1.

Drug Metab Pers Ther 2021 Apr 6. Epub 2021 Apr 6.

Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, College of Pharmacy, University of Florida, Gainesville, FL, USA.

Objectives: The function and expression of cytochrome P450 (CYP) drug metabolizing enzymes is highly variable, greatly affecting drug exposure, and therapeutic outcomes. The expression of these enzymes is known to be controlled by many transcription factors (TFs), including ligand-free estrogen receptor alpha (ESR1, in the absence of estrogen). However, the relationship between the expression of ESR1, other TFs, and CYP enzymes in human liver is still unclear. Read More

View Article and Full-Text PDF

The CCL28 levels are elevated in the serum of patients with irritable bowel syndrome and associated with the clinical symptoms.

Am J Clin Exp Immunol 2021 15;10(1):23-29. Epub 2021 Feb 15.

Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences Sanandaj, Iran.

Background: Inflammation and inflammatory mediators have been proposed to be key players in the pathobiology of Irritable bowel syndrome (IBS. The chemokine CCL28 plays a role in the trafficking of inflammatory cells into mucosal tissues. However, its levels in patients with IBS has not been yet elucidated. Read More

View Article and Full-Text PDF
February 2021

Ex-Vivo Pharmacological Defatting of the Liver: A Review.

J Clin Med 2021 Mar 18;10(6). Epub 2021 Mar 18.

Department of Hepatobiliary Surgery and Liver Transplantation, Sorbonne Université, Hôpital Pitié-Salpêtrière, Assistance Publique-Hopitaux de Paris, 75013 Paris, France.

The ongoing organ shortage has forced transplant teams to develop alternate sources of liver grafts. In this setting, ex-situ machine perfusion has rapidly developed as a promising tool to assess viability and improve the function of organs from extended criteria donors, including fatty liver grafts. In particular, normothermic machine perfusion represents a powerful tool to test a liver in full 37 °C metabolism and add pharmacological corrections whenever needed. Read More

View Article and Full-Text PDF

Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation.

Int J Mol Sci 2021 Mar 10;22(6). Epub 2021 Mar 10.

Institute of Surgical Research, University of Szeged, 6724 Szeged, Hungary.

Allograft ischemia during liver transplantation (LT) adversely affects the function of mitochondria, resulting in impairment of oxidative phosphorylation and compromised post-transplant recovery of the affected organ. Several preservation methods have been developed to improve donor organ quality; however, their effects on mitochondrial functions have not yet been compared. This study aimed to summarize the available data on mitochondrial effects of graft preservation methods in preclinical models of LT. Read More

View Article and Full-Text PDF

A Successful Urgent Liver Retransplant From a Donor With a Left Ventricular Assist Device.

Exp Clin Transplant 2021 Mar 31. Epub 2021 Mar 31.

From the the Department of General Surgery and Transplantation, ASST Grande Ospedale Metropolitano Niguarda, Milan.

Organ shortage is one of the major limitations in the field of liver transplantation, which has led to the consideration of extended criteria donors as a way to expand the donor pool. The use of extended criteria donors in cases of high Model for End-Stage Liver Disease scores or urgent recipients could be complicated by increased postoperative mortality. Donors on left ventricular assist devices could be considered extended criteria donors because of the mechanical circulatory support itself and the potential of chronic liver damage due to right ventricular failure, but experiences in the literature are limited. Read More

View Article and Full-Text PDF

Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario.

Front Surg 2021 16;8:621525. Epub 2021 Mar 16.

Transplantation and Immunology Section, Department of Surgery, Yale University, New Haven, CT, United States.

Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Read More

View Article and Full-Text PDF

The impact of hepatitis B surface antigen on natural killer cells in patients with chronic hepatitis B infection.

Liver Int 2021 Apr 1. Epub 2021 Apr 1.

Department of Gastroenterology and Hepatology, Essen University Hospital, University of Duisburg-Essen, Essen, Germany.

Background & Aims: During chronic hepatitis B virus (HBV) infection, suppressed functionality of natural killer (NK) cells might contribute to HBV persistence but the underlying mechanisms remain elusive. A peculiar feature of HBV is the secretion of large amount of hepatitis B surface antigen (HBsAg). However, the effect of HBsAg quantities on NK cells is unclear. Read More

View Article and Full-Text PDF

The First Two Liver Transplantations in Syria.

Fadi Rayya

Case Rep Gastroenterol 2021 Jan-Apr;15(1):296-304. Epub 2021 Mar 11.

General and HPB Surgery, Al Assad University Hospital, Faculty of Medicine, Damascus University, Damascus, Syrian Arab Republic.

Liver transplantation (LT) is the only curative therapy for the end-stage liver diseases and some metabolic disorders which affect the hepatic cell like the Crigler-Najjar syndrome type 1 (CNSI). Although the LT is a routine procedure in many centers worldwide, the postoperative complications such as rejection, arterial thrombosis, and infection remain serious challenges even in big centers. In our paper, we demonstrate the first two LTs in Syria. Read More

View Article and Full-Text PDF

Synthesis, characterization and in vivo distribution of intracellular delivered macrolide SCFA derivatives.

ChemMedChem 2021 Mar 31. Epub 2021 Mar 31.

Synovo GmbH, None, Paul-Ehrlich-Str., 15, 72076, Deutschland, Tübingen, GERMANY.

SCFA have a range of effects in metabolism and immune regulation. We have observed that delivery of SCFA to lysosomes, has potent immune regulatory effects, possibly as a surrogate signal for the presence of anaerobic organisms. To better understand the pharmacology of lysosomal SCFA donors, we investigated the distribution and metabolism of propionate and butyrate donors. Read More

View Article and Full-Text PDF

Peginterferon Lambda-1a for treatment of outpatients with uncomplicated COVID-19: a randomized placebo-controlled trial.

Nat Commun 2021 03 30;12(1):1967. Epub 2021 Mar 30.

Department of Medicine, Stanford University, Stanford, CA, USA.

Type III interferons have been touted as promising therapeutics in outpatients with coronavirus disease 2019 (COVID-19). We conducted a randomized, single-blind, placebo-controlled trial (NCT04331899) in 120 outpatients with mild to moderate COVID-19 to determine whether a single, 180 mcg subcutaneous dose of Peginterferon Lambda-1a (Lambda) within 72 hours of diagnosis could shorten the duration of viral shedding (primary endpoint) or symptoms (secondary endpoint). In both the 60 patients receiving Lambda and 60 receiving placebo, the median time to cessation of viral shedding was 7 days (hazard ratio [HR] = 0. Read More

View Article and Full-Text PDF

Microbiota engraftment after faecal microbiota transplantation in obese subjects with type 2 diabetes: a 24-week, double-blind, randomised controlled trial.

Gut 2021 Mar 30. Epub 2021 Mar 30.

Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

Objective: The impact of faecal microbiota transplantation (FMT) on microbiota engraftment in patients with metabolic syndrome is uncertain. We aimed to study whether combining FMT with lifestyle modification could enhance the engraftment of favourable microbiota in obese patients with type 2 diabetes mellitus (T2DM).

Design: In this double-blind, randomised, placebo-controlled trial, 61 obese subjects with T2DM were randomly assigned to three parallel groups: FMT plus lifestyle intervention (LSI), FMT alone, or sham transplantation plus LSI every 4 weeks for up to week 12. Read More

View Article and Full-Text PDF

Conditioning the Liver into a Favourable Niche for Pancreatic Islet Engraftment.

Am J Transplant 2021 Mar 30. Epub 2021 Mar 30.

Department of Surgery, University of Alberta, Edmonton, Canada.

Despite marked progress in clinical islet and pancreas transplantation, these approaches are restricted to patients with life-threatening hypoglycemic unawareness, due to the need for lifelong immunosuppression to prevent organ-rejection and shortage of donor organ availability. In the case of islet transplantation the need for multiple donors and suboptimal long-term outcomes are often due to the heterogeneity of islet preparations. For decades intrahepatic infusion remains the only clinical route of islet delivery to routinely yield in post-transplant insulin independence. Read More

View Article and Full-Text PDF

Impact of the COVID-19 pandemic on liver donation and transplantation: A review of the literature.

World J Gastroenterol 2021 Mar;27(10):928-938

Department of General Surgery and Transplantation, ASST Grande Ospedale Metropolitano Niguarda, Milan 20162, Italy.

The coronavirus disease 2019 (COVID-19) pandemic has upended healthcare systems worldwide and led to an inevitable decrease in liver transplantation (LT) activity. During the first pandemic wave, administrators and clinicians were obliged to make the difficult decision of whether to suspend or continue a life-saving procedure based on the scarce available evidence regarding the risk of transmission and mortality in immunosuppressed patients. Those centers where the activity continued or was heavily restricted were obliged to screen donors and recipients, design COVID-safe clinical pathways, and promote telehealth to prevent nosocomial transmission. Read More

View Article and Full-Text PDF

Single-cell atlas of hepatic T cells reveals expansion of liver-resident naive-like CD4+ T cells in primary sclerosing cholangitis.

J Hepatol 2021 Mar 24. Epub 2021 Mar 24.

I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg 20246 Germany; Hamburg Center for Translational Immunology, University Medical Center Hamburg-Eppendorf, Hamburg 20246 Germany; Martin Zeitz Center for Rare Diseases, University Medical Center Hamburg-Eppendorf, Hamburg 20246 Germany. Electronic address:

Background And Aims: Little is known on the composition of intrahepatic immune cells and their contribution to the pathogenesis of primary sclerosing cholangitis (PSC). We here aimed to create an atlas of intrahepatic T cells and thereby in detail characterize T cells in human inflamed liver.

Methods: Different single-cell RNA sequencing methods were combined with in silico analyses on intrahepatic and peripheral T cells from patients with PSC (n=11) and healthy donors (HD, n=4). Read More

View Article and Full-Text PDF

High-throughput transcriptomic analysis of human primary hepatocyte spheroids exposed to per- and polyfluoroalkyl substances (PFAS) as a platform for relative potency characterization.

Toxicol Sci 2021 Mar 27. Epub 2021 Mar 27.

Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch (HECSB) Health Canada.

Per- and poly-fluoroalkyl substances (PFAS) are widely found in the environment because of their extensive use and persistence. Although several PFAS are well studied, most lack toxicity data to inform human health hazard and risk assessment. This study focussed on four model PFAS: perfluorooctanoic acid (PFOA; 8 carbon), perfluorobutane sulfonate (PFBS; 4 carbon), perfluorooctane sulfonate (PFOS; 8 carbon), and perfluorodecane sulfonate (PFDS; 10 carbon). Read More

View Article and Full-Text PDF

Successful auxiliary two-staged partial resection liver transplantation (ASPIRE-LTx) for end-stage liver disease to avoid small-for-size situations.

BMC Surg 2021 Mar 26;21(1):166. Epub 2021 Mar 26.

Department of Surgery, University Medical Center Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.

Background: Risks for living-liver donors are lower in case of a left liver donation, however, due to lower graft volume, the risk for small-for-size situations in the recipients increases. This study aims to prevent small-for-size situations in recipients using an auxiliary two-staged partial resection liver transplantation (LTX) of living-donated left liver lobes.

Case Presentation: Two patients received a two-stage auxiliary LTX using living-donated left liver lobes after left lateral liver resection. Read More

View Article and Full-Text PDF

Change in hepatic volume profile in potential live liver donors after lifestyle modification for reduction of hepatic steatosis.

Abdom Radiol (NY) 2021 Mar 25. Epub 2021 Mar 25.

Division of Liver Transplantation and Hepatobiliary Surgery, Departments of Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea.

Purpose: We aimed to evaluate changes in hepatic volume and hemiliver volume percentage in potential liver donors after hepatic steatosis (HS) reduction through lifestyle modification.

Methods: Fifty liver donor candidates with HS (macrovesicular fat [MaF] ≥ 20%) underwent abdominal computed tomography (CT) and liver biopsy before (baseline) and after (follow-up) lifestyle modification. According to the change in MaF, subjects were classified as group A (MaF reduction ≥ 20%, n = 25), and group B (MaF reduction < 20%, n = 25). Read More

View Article and Full-Text PDF

An evaluation of the safety and preliminary efficacy of peri- and post- operative treprostinil in preventing ischemia and reperfusion injury in adult orthotopic liver transplant recipients.

Clin Transplant 2021 Mar 25:e14298. Epub 2021 Mar 25.

Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh.

Orthotopic liver transplantation (OLT) is the only treatment option for various end-stage liver diseases. Ischemia and reperfusion (I/R) injury is one of the unavoidable complications/conditions in OLT. In 2019, a total of 8896 livers were transplanted of which >94% organs were procured from deceased donors. Read More

View Article and Full-Text PDF

[Machine Perfusion for Liver Transplantation - What is Possible and Where Do We Stand in Germany? Review of the Literature and Results of a National Survey].

Zentralbl Chir 2021 Mar 24. Epub 2021 Mar 24.

Allgemein-, Viszeral- und Transplantationschirurgie, Medizinische Hochschule Hannover, Deutschland.

Machine perfusion of donor livers is currently regarded as the most important innovation in transplant surgery to address the continuing shortage of organs in liver transplantation. Hypothermic machine perfusion (HMP) is safe to use and appears to reduce the risk of biliary complications and improve the long-term survival of transplanted organs following preservation by cold static storage - even in donors after cardiac death. A potential functional test of donor organs during HMP uses flavin mononucleotide and is still under clinical investigation. Read More

View Article and Full-Text PDF

Commentary on "Making Every Liver Count".

Ann Surg 2020 09;272(3):402

The Johns Hopkins Hospital, Baltimore, Maryland.

View Article and Full-Text PDF
September 2020

Circulating CXCL10 and IL-6 in solid organ donors after brain death predict graft outcomes.

Sci Rep 2021 Mar 23;11(1):6624. Epub 2021 Mar 23.

Transplant Coordination Unit, Fondazione Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy.

We tested the hypothesis that circulating CXCL10 and IL-6 in donor after brain death provide independent additional predictors of graft outcome. From January 1, 2010 to June 30, 2012 all donors after brain death managed by the NITp (n = 1100) were prospectively included in this study. CXCL10 and IL-6 were measured on serum collected for the crossmatch at the beginning of the observation period. Read More

View Article and Full-Text PDF

Acellular matrix derived from rat liver improves the functionality of rat pancreatic islets before or after vitrification.

Cryobiology 2021 Mar 20. Epub 2021 Mar 20.

Graduate School of Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan; Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan. Electronic address:

Cryopreservation of pancreatic islets can overcome the severe shortage of islet donors in clinical islet transplantation, but the impaired quality of post-warm islets need improvement. This present study was conducted to investigate whether the pre- or post-treatment of rat islets with liver decellularized matrix (LDM) for vitrification can improve the viability (FDA/PI double staining) and the functionality (glucose-stimulated insulin secretion [GSIS] assay). Rat LDM was prepared by high-hydrostatic pressure, lyophilization, and re-suspension in saline. Read More

View Article and Full-Text PDF

Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid enhanced magnetic resonance imaging-guided risk assessment in living donor liver transplant patients with postoperative complications: a pilot study.

Eur Rev Med Pharmacol Sci 2021 Mar;25(5):2199-2205

Department of Radiology, Harran University Faculty of Medicine, Sanliurfa, Turkey.

Objective: To evaluate whether gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) enhanced magnetic resonance imaging (MRI), the measurements of quantitative and qualitative parameters on hepatobiliary phase images can predict the risk of postoperative complications in patients underwent living donor liver transplantation (LDLT) PATIENTS AND METHODS: We obtained Gd-EOB-DTPA-enhanced 3 Tesla MRI before living donor hepatectomy in donors (donor group; n=30) and after LDLT in their recipients (recipient group; n=30). MRIs were evaluated in terms of quantitative and qualitative variables. Quantitative parameters included relative liver enhancement value, biliary signal intensity value, and muscle signal index value. Read More

View Article and Full-Text PDF

Current review of machine perfusion in liver transplantation from the Japanese perspective.

Surg Today 2021 Mar 22. Epub 2021 Mar 22.

Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.

In light of the present evidence, machine perfusion is opening up new horizons in the field of liver transplantation. Although many advances have been made in liver transplantation, organ preservation methods have so far changed very little. Static cold storage is universally used for graft preservation in liver transplantation; however, there is a need for better preservation methods, such as ex vivo machine perfusion, to improve the outcomes by decreasing warm ischemic damage. Read More

View Article and Full-Text PDF

Anatomical variations of the hepatic artery: a closer view of rare unclassified variants.

Folia Morphol (Warsz) 2021 Mar 22. Epub 2021 Mar 22.

Liver Transplantation Department, Koc University Hospital, Istanbul, Turkey.

Background: Defining the hepatic artery anatomy is of great importance for both surgeons and radiologists. Michel classification was designed to classify hepatic artery variations. Nevertheless, there are variations that do not fit into this classification. Read More

View Article and Full-Text PDF

Systematic Assessment of Safety Threshold for Donor Age in Cadaveric Liver Transplantation.

Front Med (Lausanne) 2021 4;8:596552. Epub 2021 Mar 4.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Donor age affects allograft quality and the prognosis of recipients after liver transplantation (LT). Clinicians have assessed the quality of grafts from older donors based on their appearance and texture, with no reliable quantitative evidence. Our study aimed to assess the quantitative impact of donor age on post-transplant outcomes and its safety threshold for LT, based on the published literature. Read More

View Article and Full-Text PDF