9,184 results match your criteria liquid biopsies

Blood biomarkers for differential diagnosis and early detection of pancreatic cancer.

Cancer Treat Rev 2021 Mar 24;96:102193. Epub 2021 Mar 24.

Division of Functional Genome Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

Pancreatic cancer is currently the most lethal tumor entity and case numbers are rising. It will soon be the second most frequent cause of cancer-related death in the Western world. Mortality is close to incidence and patient survival after diagnosis stands at about five months. Read More

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Detection of activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted next-generation sequencing: a pilot study.

J Egypt Natl Canc Inst 2021 Apr 17;33(1):10. Epub 2021 Apr 17.

Clinical Oncology Department, School of Medicine, Cairo University, Cairo, Egypt.

Background: Breast cancer (BC) is the 2 most prevalent malignancy worldwide and is the most prevalent cancer among Egyptian women. The number of newly described cancer-associated genes has grown exponentially since the emergence of next-generation sequencing (NGS) technology. We aim to identify activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted NGS technology. Read More

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A verified genomic reference sample for assessing performance of cancer panels detecting small variants of low allele frequency.

Genome Biol 2021 Apr 16;22(1):111. Epub 2021 Apr 16.

Division of Bioinformatics and Biostatistics, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, 72079, USA.

Background: Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially in clinical trials. Currently, there is a paucity of reliable genomic reference samples having a suitably large number of pre-identified variants for properly assessing oncopanel assay analytical quality and performance. The FDA-led Sequencing and Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually and their pool, termed Sample A, to develop a reference sample with suitably large numbers of coding positions with known (variant) positives and negatives for properly evaluating oncopanel analytical performance. Read More

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Comparison between two cell collecting methods for liquid-based brush biopsies: a consecutive and retrospective study.

BMC Oral Health 2021 Apr 16;21(1):195. Epub 2021 Apr 16.

Department of Oral, Maxillofacial and Facial Plastic Surgery, Section of Clinical and Experimental Oral Medicine, Leipzig University Hospital, Liebigstraße 10-14, 04103, Leipzig, Germany.

Background: This study compares two different cell collectors, the Orcellex Brush (rigid brush) and the Cytobrush GT (nylon brush), using liquid-based cytology. A comparison of their obtainment procedures was also considered. The aim was to determine the diagnostic accuracy for detection of malignancy in oral brush biopsies. Read More

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Circulating Tumor Cell Detection Methods in Renal Cell Carcinoma: A Systematic Review.

Crit Rev Oncol Hematol 2021 Apr 13:103331. Epub 2021 Apr 13.

Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028, Lisboa, Portugal; Oncology Department, Centro Hospitalar Universitário Lisboa Norte, Av. Prof. Egas Moniz, 1649-028, Lisboa, Portugal; Instituto de Medicina Molecular João Lobo Antunes, Av. Prof. Egas Moniz, 1649-028, Lisboa, Portugal.

Circulating tumor cells (CTCs) have a potential role as the missing renal cell carcinoma (RCC) biomarker. However, the available evidence is limited, and detection methods lack standardization, hindering clinical use. We performed a systematic review on CTC enrichment and detection methods, and its role as a biomarker in RCC. Read More

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Serum Bile Acid, Vitamin E, and Serotonin Metabolites Are Associated With Future Liver-Related Events in Nonalcoholic Fatty Liver Disease.

Hepatol Commun 2021 Apr 5;5(4):608-617. Epub 2021 Jan 5.

Division of Gastroenterology Department of Medicine Duke University Hospital Durham NC USA.

Identifying patients at higher risk for poor outcomes from nonalcoholic fatty liver disease (NAFLD) remains challenging. Metabolomics, the comprehensive measurement of small molecules in biological samples, has the potential to reveal novel noninvasive biomarkers. The aim of this study was to determine if serum metabolite profiles in patients with NAFLD associate with future liver-related events. Read More

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Digital next-generation sequencing of cell-free DNA for pancreatic cancer.

JGH Open 2021 Apr 22;5(4):508-516. Epub 2021 Mar 22.

First Department of Internal Medicine, Faculty of Medicine University of Yamanashi Chuo Japan.

Background And Aim: The clinical applicability of digital next-generation sequencing (dNGS), which eliminates polymerase chain reaction (PCR) and sequencing error-derived noise by using molecular barcodes (MBs), has not been fully evaluated. We evaluated the utility of dNGS of cell-free DNA (cfDNA) in liquid biopsies obtained from patients with pancreatic cancer.

Methods: Fifty-eight patients with pancreatic cancer undergoing endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) were included. Read More

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A combination of surgery, theranostics, and liquid biopsy - a personalised oncologic approach to treatment of patients with advanced metastatic neuroendocrine neoplasms.

Int J Med Sci 2021 19;18(10):2166-2175. Epub 2021 Mar 19.

CURANOSTICUM Wiesbaden-Frankfurt at DKD Helios Klinik, Wiesbaden, Germany.

Neuroendocrine neoplasia (NEN) of small bowel (SBNEN) frequently present with metastatic disease. Theranostics (molecular imaging followed by targeting therapy) allow for personalised medicine. Liquid biopsies enable precise identification of residual disease and real-time monitoring of therapeutic response. Read More

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The role of extracellular DNA (exDNA) in cellular processes.

Cancer Biol Ther 2021 Apr 16:1-12. Epub 2021 Apr 16.

Division of Basic Research, Instituto Nacional de Cancerología, México City.

Nowadays, extracellular DNA or circulating cell-free DNA is considered to be a molecule with clinical applications (diagnosis, prognosis, monitoring of treatment responses, or patient follow-up) in diverse pathologies, especially in cancer. Nevertheless, because of its molecular characteristics, it can have many other functions. This review focuses on the participation of extracellular DNA (exDNA) in fundamental processes such as cell signaling, coagulation, immunity, evolution through horizontal transfer of genetic information, and adaptive response to inflammatory processes. Read More

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KRAS Mutational Regression Is Associated With Oligo-Metastatic Status and Good Prognosis in Metastatic Colorectal Cancer.

Front Oncol 2021 29;11:632962. Epub 2021 Mar 29.

Department of Precision Medicine, University of Campania "L. Vanvitelli", Naples, Italy.

Background: We previously reported that loss of mutations ("regressive" mutational trajectories) from primary tumors to metastases associated with the oligo-metastatic status in colorectal cancer (CRC). The present study was undertaken in order to analyze the mutational trajectories of in a well-characterized cohort of CRC patients who developed poly- or oligo-metastatic disease.

Material And Methods: Patients were treated and followed-up according to European Society of Medical Oncology guidelines. Read More

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Cellular Mechanism of Gene Mutations and Potential Therapeutic Targets in Ovarian Cancer.

Cancer Manag Res 2021 8;13:3081-3100. Epub 2021 Apr 8.

Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.

Ovarian cancer is a common and complex malignancy with poor prognostic outcome. Most women with ovarian cancer are diagnosed with advanced stage disease due to a lack of effective detection strategies in the early stage. Traditional treatment with cytoreductive surgery and platinum-based combination chemotherapy has not significantly improved prognosis and 5-year survival rates are still extremely poor. Read More

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Role of hepatitis D virus infection in development of hepatocellular carcinoma among chronic hepatitis B patients treated with nucleotide/nucleoside analogues.

Sci Rep 2021 Apr 14;11(1):8184. Epub 2021 Apr 14.

Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Tzyou Road, Kaohsiung, 807, Taiwan.

Hepatitis D virus (HDV) infection increases the risk of hepatocellular carcinoma (HCC) in the natural course of chronic hepatitis B (CHB) patients. Its role in patients treated with nucleotide/nucleoside analogues (NAs) is unclear. We aimed to study the role of hepatitis D in the development of HCC in CHB patients treated with NAs. Read More

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The IMPACT trial: human papillomavirus, cervical cytology and histopathological results from the baseline and 1-year follow-up phase.

Am J Obstet Gynecol 2021 Apr 11. Epub 2021 Apr 11.

Ventana Medical Systems Inc./Roche Tissue Diagnostics, Tucson, AZ.

Background: An increase in human papillomavirus (HPV) test volumes is expected in the near future as HPV-based screening protocols are expected to become more broadly adopted.

Objective: The IMproving Primary screening And Colposcopy Triage (IMPACT) trial, a prospective, multicenter US cervical cancer screening trial, was conducted to obtain FDA approvals for the new high-throughput cobas HPV for use on the cobas 6800/8800 Systems (cobas HPV) for detecting cervical precancer and cancer (cervical intraepithelial neoplasia of grade 2 or worse [≥CIN2] and grade 3 or worse, [≥CIN3]). Here the baseline demographics, HPV, cervical cytology and histolpathologic results are presented. Read More

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Anti-Tim4 Grafting Strongly Hydrophilic Metal-Organic Frameworks Immunoaffinity Flake for High-Efficiency Capture and Separation of Exosomes.

Anal Chem 2021 Apr 14. Epub 2021 Apr 14.

College of Pharmaceutical Sciences, Key Laboratory of Public Health Safety of Hebei Province, Hebei University, Baoding 071002, China.

Exosomes have become the most ideal analysis target for liquid biopsy since they carry a large amount of genetic materials. The study on exosomes has great significance for cancer diagnosis and prognosis. However, the extremely low concentration renders the development of a robust exosomes enrichment technique, with the merits of low nonspecific cell adhesion, high-capture efficiency, and easy nondestructive release of captured exosomes, of vital significance. Read More

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Detection of circulating tumor cells: Advances and critical concerns.

Oncol Lett 2021 May 29;21(5):422. Epub 2021 Mar 29.

Clinical Medical Laboratory, Huai'an Maternity and Child Health Care Hospital, Huai'an, Jiangsu 223002, P.R. China.

Metastasis is the main cause of cancer-related death and the major challenge in cancer treatment. Cancer cells in circulation are termed circulating tumor cells (CTCs). Primary tumor metastasis is likely due to CTCs released into the bloodstream. Read More

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A multiparametric extraction method for Vn96-isolated plasma extracellular vesicles and cell-free DNA that enables multi-omic profiling.

Sci Rep 2021 Apr 13;11(1):8085. Epub 2021 Apr 13.

Atlantic Cancer Research Institute, 35 Providence St., Moncton, NB, E1C 8X3, Canada.

Extracellular vesicles (EVs) have been recognized as a rich material for the analysis of DNA, RNA, and protein biomarkers. A remaining challenge for the deployment of EV-based diagnostic and prognostic assays in liquid biopsy testing is the development of an EV isolation method that is amenable to a clinical diagnostic lab setting and is compatible with multiple types of biomarker analyses. We have previously designed a synthetic peptide, known as Vn96 (ME kit), which efficiently isolates EVs from multiple biofluids in a short timeframe without the use of specialized lab equipment. Read More

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Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids.

Sci Rep 2021 Apr 13;11(1):8034. Epub 2021 Apr 13.

William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA.

Extracellular vesicles (EVs) derived from tumor cells have the potential to provide a much-needed source of non-invasive molecular biomarkers for liquid biopsies. However, current methods for EV isolation have limited specificity towards tumor-derived EVs that limit their clinical use. Here, we present an approach called immunomagnetic sequential ultrafiltration (iSUF) that consists of sequential stages of purification and enrichment of EVs in approximately 2 h. Read More

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Integrated approaches for precision oncology in colorectal cancer: the more you know, the better.

Semin Cancer Biol 2021 Apr 10. Epub 2021 Apr 10.

Department of Oncology, University of Torino, Str. Prov.le 142, km. 3,95, 10060, Candiolo, TO, Italy; Candiolo Cancer Institute, FPO - IRCCS, Str. Prov.le 142, km. 3,95, Candiolo, TO, 10060, Italy. Electronic address:

Colorectal cancer (CRC) is one of the most common human malignancies accounting for approximately 10% of worldwide cancer incidence and mortality. While early-stage CRC is mainly a preventable and curable disease, metastatic colorectal cancer (mCRC) remains an unmet clinical need. Moreover, about 25% of CRC cases are diagnosed only at the metastatic stage. Read More

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The role and potential application of extracellular vesicles in liver cancer.

Sci China Life Sci 2021 Apr 9. Epub 2021 Apr 9.

Cancer Research Center, The First Affiliated Hospital, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China.

Liver cancer is one of the most common causes of cancer-related death worldwide and mainly includes hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Extracellular vesicles (EVs) are membrane-derived nanometer-sized vesicles that can be released by different cell types under normal and pathological conditions and thus play important roles in the transmission of biological information between cells. Increasing evidence suggests that liver cancer cell-derived EVs may help establish a favorable microenvironment to support the proliferation, invasion and metastasis of cancer cells. Read More

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Mutations as a Molecular Target for Hormone Receptor-Positive, HER2-Negative Metastatic Breast Cancer.

Front Oncol 2021 25;11:644737. Epub 2021 Mar 25.

Division of Pathology, IEO, European Institute of Oncology IRCCS, Milan, Italy.

Despite the significant achievements in the diagnosis and treatment of metastatic breast cancer (MBC), this condition remains substantially an incurable disease. In recent years, several clinical studies have aimed to identify novel molecular targets, therapeutic strategies, and predictive biomarkers to improve the outcome of women with MBC. Overall, ~40% of hormone receptor (HR)/HER2 MBC cases harbor alterations affecting the (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway. Read More

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High-Throughput and Automated Acoustic Trapping of Extracellular Vesicles to Identify microRNAs With Diagnostic Potential for Prostate Cancer.

Front Oncol 2021 25;11:631021. Epub 2021 Mar 25.

Department of Laboratory Medicine, Lund University, Lund, Sweden.

Molecular profiling of extracellular vesicles (EVs) offers novel opportunities for diagnostic applications, but the current major obstacle for clinical translation is the lack of efficient, robust, and reproducible isolation methods. To bridge that gap, we developed a microfluidic, non-contact, and low-input volume compatible acoustic trapping technology for EV isolation that enabled downstream small RNA sequencing. In the current study, we have further automated the acoustic microfluidics-based EV enrichment technique that enables us to serially process 32 clinical samples per run. Read More

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The feasibility of circulating tumor DNA analysis as a marker of recurrence in triple-negative breast cancer.

Oncol Lett 2021 May 28;21(5):420. Epub 2021 Mar 28.

Respiratory Center, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.

Triple-negative breast cancer (TNBC) has a poorer prognosis than other breast cancer subtypes; therefore, identifying markers of early recurrence is important. The present study aimed to establish a liquid biopsy protocol for droplet digital PCR-based detection of frequently mutated genes in patients with TNBC. Tumor DNA from 36 patients with TNBC who relapsed within 2 years after surgical resection was retrospectively analyzed. Read More

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Narrative review of the influence of diabetes mellitus and hyperglycemia on colorectal cancer risk and oncological outcomes.

Transl Oncol 2021 Apr 7;14(7):101089. Epub 2021 Apr 7.

Division of Colorectal Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, No. 100 Tzyou 1st Road, Kaohsiung City 807, Kaohsiung, Taiwan; Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Taiwan; Graduate Institute of Medicine, Kaohsiung Medical University, Taiwan; Center for Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan; Center for Liquid Biopsy and Cohort Research, Taiwan. Electronic address:

Diabetes mellitus (DM) and hyperglycemia have been shown to have significant effects on the incidence, chemoresistance, and prognosis of colorectal cancer (CRC), as well as the outcomes of localized and metastatic CRC. Inflammation and endocrine effects may act as central mechanisms of DM and cancer and stimulate the insulin-like growth factor 1-phosphoinositide 3-kinase-Akt-mammalian target of rapamycin (IGF-1-PI3K-AKT-mTOR) pathway. Dysregulation of the AMP-activated protein kinase (AMPK) pathway leads to metabolic imbalance and indicates cancer risk. Read More

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Hexokinase 2 discerns a novel circulating tumor cell population associated with poor prognosis in lung cancer patients.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Institute for Systems Biology, Seattle, WA 98109;

Unlike other epithelial cancer types, circulating tumor cells (CTCs) are less frequently detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients using epithelial marker-based detection approaches despite the aggressive nature of NSCLC. Here, we demonstrate hexokinase-2 (HK2) as a metabolic function-associated marker for the detection of CTCs. In 59 NSCLC patients bearing cytokeratin-positive (CK) primary tumors, HK2 enables resolving cytokeratin-negative (HK2/CK) CTCs as a prevalent population in about half of the peripheral blood samples with positive CTC counts. Read More

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Changing Pattern of Chemsex Drug Use Among Newly Diagnosed HIV-Positive Taiwanese from 2015 to 2020 in the Era of Treat-All Policy.

AIDS Patient Care STDS 2021 04;35(4):134-143

Department of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Chemsex drug use (CDU) is a frequent, yet neglected issue in the era of treat-all policy. We evaluated the temporal pattern of CDU, factors associated with CDU, and drug-drug interactions (DDIs) between chemsex drugs and initial antiretroviral therapy (ART) by surveying 621 Taiwanese individuals (mean age: 29.7 years; 99. Read More

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Micro-RNA Quantification, Target Gene Identification, and Pathway Analysis.

Methods Mol Biol 2021 ;2284:207-229

Department of Biology, University of Padova, Padova, Italy.

RNA sequencing has become a powerful tool for profiling the expression level of small RNAs from both solid tissues and liquid biopsies. In conjunction with pathway analysis, it offers exciting possibilities for the identification of disease specific biomarkers. In this chapter, we describe a workflow for processing this type of sequencing data. Read More

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January 2021

Horizons in Veterinary Precision Oncology: Fundamentals of Cancer Genomics and Applications of Liquid Biopsy for the Detection, Characterization, and Management of Cancer in Dogs.

Front Vet Sci 2021 23;8:664718. Epub 2021 Mar 23.

PetDx, La Jolla, CA, United States.

Cancer is the leading cause of death in dogs, in part because many cases are identified at an advanced stage when clinical signs have developed, and prognosis is poor. Increased understanding of cancer as a disease of the genome has led to the introduction of liquid biopsy testing, allowing for detection of genomic alterations in cell-free DNA fragments in blood to facilitate earlier detection, characterization, and management of cancer through non-invasive means. Recent discoveries in the areas of genomics and oncology have provided a deeper understanding of the molecular origins and evolution of cancer, and of the "one health" similarities between humans and dogs that underlie the field of comparative oncology. Read More

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Circulating miR-320a Acts as a Tumor Suppressor and Prognostic Factor in Non-small Cell Lung Cancer.

Front Oncol 2021 23;11:645475. Epub 2021 Mar 23.

Department of Zoology, Central University of Punjab, Bathinda, India.

Dysregulated expression profiles of microRNAs (miRNAs) have been observed in several types of cancer, including non-small cell lung cancer (NSCLC); however, the diagnostic and prognostic potential of circulating miRNAs in NSCLC remains largely undefined. Here we found that circulating miR-320a was significantly down-regulated (~5.87-fold; < 0. Read More

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Serum Exosomal miRNA Might Be a Novel Liquid Biopsy to Identify Leptomeningeal Metastasis in Non-Small Cell Lung Cancer.

Onco Targets Ther 2021 1;14:2327-2335. Epub 2021 Apr 1.

Department of Respiratory and Critical Care Medicine, Affiliated Jinling Hospital, Southeast University, Sch Med, Nanjing, Jiangsu, People's Republic of China.

Purpose: The survival time of patients with leptomeningeal metastasis (LM) of lung cancer is very short, and the clinical characteristics of LM are varied, making the clinical diagnosis difficult. At present, a positive CSF fluid (CSF) cytology result remains the gold standard for diagnosing LM in lung cancer; however, the process of collecting CSF is traumatic and far less convenient than blood collection. With the development in technology, an increasing number of studies prefer to use liquid biopsy to diagnose or predict the occurrence of the disease. Read More

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Epigenetics, fragmentomics, and topology of cell-free DNA in liquid biopsies.

Science 2021 04;372(6538)

Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.

Liquid biopsies that analyze cell-free DNA in blood plasma are used for noninvasive prenatal testing, oncology, and monitoring of organ transplant recipients. DNA molecules are released into the plasma from various bodily tissues. Physical and molecular features of cell-free DNA fragments and their distribution over the genome bear information about their tissues of origin. Read More

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