4,175 results match your criteria lipolytic activity


Technological characterization of indigenous lactic acid bacteria from Moroccan camel milk for their potential use as starter or adjunct culture.

Folia Microbiol (Praha) 2021 Jun 10. Epub 2021 Jun 10.

RU Animal Production and Forage, Food Technology Laboratory, INRA, RCAR-Rabat, Institutes Rabat, 6570, 10101, Rabat, PB, Morocco.

This study aimed to isolate lactic acid bacteria (LABs) of technological interest from Moroccan camel milk and select starter or adjunct culture for dairy product manufacturing. The phenotypic and biochemical identification of 47 isolates revealed the existence of ten Lactococcus lactis, eleven Lactobacillus plantarum, three Lactobacillus brevis, two Lactobacillus paracasei, eleven Enterococcus spp., seven Lactococcus spp. Read More

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A case of tamoxifen-induced hypertriglyceridemia monitoring the changes in lipoprotein fractions over time.

BMC Endocr Disord 2021 Jun 9;21(1):115. Epub 2021 Jun 9.

Division of Diabetes, Metabolism and Endocrinology, Kawasaki Medical School, 577 Matsushima, Kurashiki, 701-0192, Japan.

Background: Tamoxifen, which is one of the selective estrogen receptor modulators (SERMs), can bring out life-threatening complication, e.g. hypertriglyceridemia-induced acute pancreatitis, although it is rare. Read More

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Removal of overpainting from an historical painting of the XVIII Century: A yeast enzymatic approach.

J Biotechnol 2021 Jun 4;335:55-64. Epub 2021 Jun 4.

Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia; Caravella, s.r.o., Tupolevova 2, 851 01 Bratislava, Slovakia. Electronic address:

Biocleaning of cultural heritage items is mainly performed using living microorganisms. Approaches utilizing the enzymes of isolated microorganisms have not been frequently investigated. To find an enzymatic alternative for the removal of an oil-based overpainting, we focused on the characterization and use of a yeast Extracellular Enzymatic Mixture (EEM). Read More

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Proteolytic and Lipolytic of Dairy Origin as Meat Functional Starter Cultures.

Food Technol Biotechnol 2021 Mar;59(1):63-73

Department of Microbiology, University of Zagreb Faculty of Agriculture, Svetošimunska cesta 25, Zagreb, Croatia.

Research Background: As fermentation is an integral feature of both, dry sausage and cheese production, this has led to the evaluation of bacterial cultures ssp. (LL8307) and (ED0207) originally isolated from artisanal Croatian hard type cheese to diversify the range of flavours of dry fermented sausages and to increase their microbiological safety. Both strains were chosen for their high or medium acidifying, proteolytic and/or lipolytic activity and bioprotective potential after step-by-step selection of wild isolates. Read More

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Improving the Bioremediation and in situ Production of Biocompounds of a Biodiesel-Contaminated Soil.

Environ Manage 2021 Jun 2. Epub 2021 Jun 2.

Faculty of Engineering and Architecture (FEAR), Postgraduate Program in Civil and Environmental Engineering (PPGEng), University of Passo Fundo (UPF), Passo Fundo, RS, Brazil.

We aimed to produce simultaneously biosurfactants and lipases in solid state fermentation (SSF) using Aspergillus niger, followed by the use of the fermented media on the bioremediation of oily contaminated soil, in order to valuate agro industrial residuals and reduce the contamination. The biocompounds were produced using wheat bran and corncob (80:20), 5% of soybean oil and 0.5% of sugar cane molasses in SSF for 4 d, producing 4. Read More

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P2Y2R Deficiency Ameliorates Hepatic Steatosis by Reducing Lipogenesis and Enhancing Fatty Acid β-Oxidation through AMPK and PGC-1α Induction in High-Fat Diet-Fed Mice.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

Department of Pharmacology, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea.

Non-alcoholic fatty liver disease (NAFLD) is a chronic metabolic liver disease associated with obesity and insulin resistance. Activation of the purinergic receptor P2Y2R has been reported to promote adipogenesis, inflammation and dyslipidemia in adipose tissues in obese mice. However, the role of P2Y2R and its mechanisms in NAFLD remain unknown. Read More

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A Milk Foodomics Investigation into the Effect of Growth under Cold Chain Conditions.

Foods 2021 May 24;10(6). Epub 2021 May 24.

Department for Sustainable Food Process (DiSTAS), Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy.

is a psychrotrophic species associated with milk spoilage because of its lipolytic and proteolytic activities. Consequently, monitoring or its antecedent activity in milk is critical to preventing quality defects of the product and minimizing food waste. Therefore, in this study, untargeted metabolomics and peptidomics were used to identify the changes in milk related to activity by simulating the low-temperature conditions usually found in milk during the cold chain. Read More

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SIRT5 Inhibition Induces Brown Fat-Like Phenotype in 3T3-L1 Preadipocytes.

Cells 2021 May 7;10(5). Epub 2021 May 7.

Laboratory of Cardiovascular Endocrinology, IRCCS San Raffaele Pisana, 00163 Rome, Italy.

Brown adipose tissue (BAT) activity plays a key role in regulating systemic energy. The activation of BAT results in increased energy expenditure, making this tissue an attractive pharmacological target for therapies against obesity and type 2 diabetes. Sirtuin 5 (SIRT5) affects BAT function by regulating adipogenic transcription factor expression and mitochondrial respiration. Read More

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Identification and characterization of a novel carboxylesterase EstQ7 from a soil metagenomic library.

Arch Microbiol 2021 May 31. Epub 2021 May 31.

Key Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

A novel lipolytic gene, estq7, was identified from a fosmid metagenomic library. The recombinant enzyme EstQ7 consists of 370 amino acids with an anticipated molecular mass of 42 kDa. Multiple sequence alignments showed that EstQ7 contained a pentapeptide motif GHSMG, and a putative catalytic triad Ser174-Asp306-His344. Read More

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Lipolysis drives expression of the constitutively active receptor GPR3 to induce adipose thermogenesis.

Cell 2021 May 22. Epub 2021 May 22.

Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

Thermogenic adipocytes possess a therapeutically appealing, energy-expending capacity, which is canonically cold-induced by ligand-dependent activation of β-adrenergic G protein-coupled receptors (GPCRs). Here, we uncover an alternate paradigm of GPCR-mediated adipose thermogenesis through the constitutively active receptor, GPR3. We show that the N terminus of GPR3 confers intrinsic signaling activity, resulting in continuous Gs-coupling and cAMP production without an exogenous ligand. Read More

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Mechanical overload-induced muscle-derived extracellular vesicles promote adipose tissue lipolysis.

FASEB J 2021 Jun;35(6):e21644

Center for Muscle Biology, University of Kentucky, Lexington, KY, USA.

How regular physical activity is able to improve health remains poorly understood. The release of factors from skeletal muscle following exercise has been proposed as a possible mechanism mediating such systemic benefits. We describe a mechanism wherein skeletal muscle, in response to a hypertrophic stimulus induced by mechanical overload (MOV), released extracellular vesicles (EVs) containing muscle-specific miR-1 that were preferentially taken up by epidydimal white adipose tissue (eWAT). Read More

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Adipocyte P2Y14 receptors play a key role in regulating whole-body glucose and lipid homeostasis.

JCI Insight 2021 May 24;6(10). Epub 2021 May 24.

Molecular Recognition Section and.

Obesity is the major driver of the worldwide epidemic in type 2 diabetes (T2D). In the obese state, chronically elevated plasma free fatty acid levels contribute to peripheral insulin resistance, which can ultimately lead to the development of T2D. For this reason, drugs that are able to regulate lipolytic processes in adipocytes are predicted to have considerable therapeutic potential. Read More

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Effect of olive leaves feeding on phenolic composition and lipolytic volatile profile in goat milk.

J Dairy Sci 2021 May 20. Epub 2021 May 20.

Faculty of BioScience and Technology for Food, Agriculture, and Environment, University of Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy. Electronic address:

The aim of this study was to evaluate phenolic composition, antioxidant potential, and lipolytic events in raw milk obtained from goat fed a dietary supplementation with olive leaves (OL), a by-product of the olive oil production chain. For this purpose, 30 Saanen goats were randomly allocated into 2 groups of 15 goats each: the control group received a standard diet that was prepared by taking into account the nutritional needs of lactating goats, whereas the experimental group (EG) was fed with an OL-supplemented diet (10% on a dry matter basis). At the end of the 30 d of the trial, the individual milk samples were collected and immediately analyzed for total phenolic content and antioxidant activity (AOA). Read More

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The lipolytic activity of LipJ, a stress-induced enzyme, is regulated by its C-terminal adenylate cyclase domain.

Future Microbiol 2021 May 7;16:487-507. Epub 2021 May 7.

Department of Biotechnology, BMS Block-1, South Campus, Panjab University, Chandigarh, India.

The confirmation of lipolytic activity and role of Rv1900c in the physiology /N-terminus domain () were cloned in pET28a/, purified by affinity chromatography and characterized. A zone of clearance on tributyrin-agar and activity with NP-decanoate confirmed the lipolytic activity of Rv1900c. The Rv1900NT demonstrated higher enzyme specific activity, V and , but Rv1900c was more thermostable. Read More

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Modest sleep restriction does not influence steps, physical activity intensity or glucose tolerance in obese adults.

J Sleep Res 2021 May 5:e13381. Epub 2021 May 5.

Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO, USA.

Sleep restriction (SR) (<6 h) and physical activity (PA) are risk factors for obesity, but little work has examined the inter-related influences of both risk factors. In a free-living environment, 13 overweight/obese adults were sleep restricted for five nights to 6 h time-in-bed each night, with and without regular exercise (45 min/65% VO max; counterbalanced design). Two days of recovery sleep followed SR. Read More

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The 2020 ESPEN Arvid Wretlind lecture: Metabolic response in bariatric surgery - Mechanisms and clinical implications.

Authors:
Anders Thorell

Clin Nutr 2021 May 20;40(5):2602-2608. Epub 2021 Apr 20.

Karolinska Institutet, Department of Clinical Science, Danderyd Hospital & Department of Surgery, Ersta Hospital, 116 91 Stockholm, Sweden. Electronic address:

Background & Aims: Owing to the "obesity-pandemic", an increasing number of individuals are in need of treatment for obesity and obesity-related disorders. For patients with severe disease, results with conventional treatment modalities such as diet regimens, physical activity, and pharmacologic agents most often lack satisfactory efficacy and/or sustainability. In contrast, bariatric surgery has been demonstrated to be associated with marked, long-term weight loss as well as resolution or improvement of co-morbid disease, in particular metabolic aberrations such as insulin resistance and type 2 diabetes. Read More

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Structure-Guided Rational Design of a Mono- and Diacylglycerol Lipase from : A Single Residue Mutant Increases the Hydrolysis Ability.

J Agric Food Chem 2021 May 30;69(18):5344-5352. Epub 2021 Apr 30.

School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China.

Engineering of enzymes on the basis of protein structures are rational and efficient approaches to acquire biocatalysts of desired performances. In this study, we focused on a special mono- and diacylglycerol lipase (MDGL) isolated from the lipolytic enzyme-enriched fungus and discovered improved variants based on its crystal structure. We first solved the crystal structure of lipase (AOL) at 1. Read More

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Application of Lipid Class Ratios for Sample Stability Monitoring-Evaluation of Murine Tissue Homogenates and SDS as a Stabilizer.

Metabolites 2021 Apr 27;11(5). Epub 2021 Apr 27.

Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany.

Lipids are a ubiquitous class of structurally complex molecules involved in various biological processes. In the fast-growing field of lipidomics, preanalytical issues are frequently neglected. Here, we investigated the stability of lipid profiles of murine liver, brain, lung, heart, and spleen homogenates by quantitative flow injection analysis using tandem mass spectrometry and high-resolution mass spectrometry. Read More

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Anti-Obesity Effect of Pine Needle Extract on High-Fat Diet-Induced Obese Mice.

Plants (Basel) 2021 Apr 21;10(5). Epub 2021 Apr 21.

Department of Physiology and Convergence Medical Science, Institute of Health Sciences, Gyeongsang National University Medical School, Jinju 52727, Korea.

Background: Obesity due to an excessive intake of nutrient disturbs the hypothalamus-mediated energy metabolism subsequently develops metabolic disorders. In this study, we investigated the effect of pine needle extract (PNE) on the hypothalamic proopiomelanocortin (POMC) neurons involved in the regulation of energy balance via melanocortin system and fat tissue metabolism.

Methods: We performed electrophysiological and immunohistochemical analyses to determine the effect of PNE on POMC neurons. Read More

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Reduction of litter size during lactation in rats greatly influences fatty acid profiles in dams.

J Physiol Biochem 2021 Apr 28. Epub 2021 Apr 28.

Department of Chemistry and Biochemistry, Universidad San Pablo CEU, Madrid, Spain.

This study aimed to determine in lactating rats how fatty acid profiles are affected by litter size. On day 2 after parturition, litters of lactating rats were adjusted to a normal litter size of 9 pups/dam (NL) or to a small litter of 4 pups/dam (SL), and dams were studied at day 21 of lactation. Plasma glucose, insulin, and docosahexaenoic acid (DHA) concentrations were higher in SL than in NL dams, whereas the concentrations of most other fatty acids, triacylglycerols (TAG), and non-esterified fatty acids were lower in the SL dams. Read More

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Carboxylesterase 2 proteins are efficient diglyceride and monoglyceride lipases possibly implicated in metabolic disease.

J Lipid Res 2021 Apr 17;62:100075. Epub 2021 Apr 17.

Institute of Molecular Biosciences, University of Graz, Graz, Austria. Electronic address:

Carboxylesterase 2 (CES2/Ces2) proteins exert established roles in (pro)drug metabolism. Recently, human and murine CES2/Ces2c have been discovered as triglyceride (TG) hydrolases implicated in the development of obesity and fatty liver disease. The murine Ces2 family consists of seven homologous genes as opposed to a single CES2 gene in humans. Read More

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Identification and Heterologous Production of a Lipase from Geobacillus kaustophilus DSM 7263 and Tailoring Its N-Terminal by a His-Tag Epitope.

Protein J 2021 Apr 15. Epub 2021 Apr 15.

Department of Molecular Biology and Genetics, Gebze Technical University, Gebze, 41400, Kocaeli, Turkey.

Lipases are versatile biocatalysts with many biotechnological applications and the necessity of screening, production and characterization of new lipases from diverse microbial strains to meet industrial needs is constantly emerging. In this study, the lipase gene (gklip) from a thermophilic bacterium, Geobacillus kaustophilus DSM 7263 was cloned into the pET28a ( +) vector with N-terminal 6xHis-tag. The recombinant gklip gene was heterologously expressed in host E. Read More

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The Occurrence of Triple Catalytic Characteristics of Yeast Lipases and Their Application Prospects in Biodiesel Production from Non-Edible Jatropha curcas Oil in a Solvent-Free System.

Curr Microbiol 2021 May 9;78(5):1914-1925. Epub 2021 Apr 9.

Molecular Biotechnology Laboratory, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.

Extracellular and cell-bound lipase-producing yeasts were isolated from the palm oil mill wastes and investigated for their potential uses as biocatalysts in biodiesel production. Twenty-six yeast strains were qualitatively screened as lipase producers. From those yeast strains, only six were selected and screened further for quantitative lipase production. Read More

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Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification.

J Genet Eng Biotechnol 2021 Apr 7;19(1):55. Epub 2021 Apr 7.

Department of Biotechnology Research and Development, PT Wilmar Benih Indonesia, Bekasi, 17530, Indonesia.

Background: Lipases are promising biocatalysts for industrial applications and attract attention to be explored. A novel acidic lipase has been isolated from the lipolytic bacteria Micrococcus luteus EMP48-D (LipEMP48-D) screened from tempeh. The lipase gene had previously been overexpressed in Escherichia coli BL21, but the expression level obtained was relatively low. Read More

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Lipase production by microorganisms isolated from the Serra de Ouro Branco State Park.

An Acad Bras Cienc 2021 31;93(1):e20190672. Epub 2021 Mar 31.

Universidade Federal de São João Del-Rei (UFSJ/ DEQUI), Departamento de Engenharia Química, Campus Alto Paraopeba, Rodovia MG 443, Km 07, 36420-000 Ouro Branco, MG, Brazil.

Lipases are hydrolases used in various sectors such as the food, pharmaceutical and chemical synthesis industries. In this study, epiphytic microorganisms were isolated from the Serra of Ouro Branco State Park (Minas Gerais, Brazil) and were subsequently evaluated for their ability to produce extracellular lipases. Among the 46 isolated strains, 25 presented positive results for lipase production in the agar plate screening assay. Read More

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Lipolytic Activity of a Carboxylesterase from Bumblebee () Venom.

Toxins (Basel) 2021 03 26;13(4). Epub 2021 Mar 26.

College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea.

Bee venom is a complex mixture composed of peptides, proteins with enzymatic properties, and low-molecular-weight compounds. Although the carboxylesterase in bee venom has been identified as an allergen, the enzyme's role as a venom component has not been previously elucidated. Here, we show the lipolytic activity of a bumblebee () venom carboxylesterase (BivCaE). Read More

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Technological and Safety Attributes of Lactic Acid Bacteria and Yeasts Isolated from Spontaneously Fermented Greek Wheat Sourdoughs.

Microorganisms 2021 Mar 24;9(4). Epub 2021 Mar 24.

Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, 11855 Athens, Greece.

The aim of the present study was to assess the technological and safety potential of 207 lactic acid bacteria (LAB) and 195 yeast strains isolated from spontaneously fermented Greek wheat sourdoughs. More accurately, the amylolytic, proteolytic, lipolytic, phytase and amino acid decarboxylase activities, along with the production of exopolysaccharides and antimicrobial compounds by the LAB and yeast isolates, were assessed. A well diffusion assay revealed seven proteolytic LAB and eight yeast strains; hydrolysis of tributyrin was evident only in 11 LAB strains. Read More

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Zymography for Picogram Detection of Lipase and Esterase Activities.

Molecules 2021 Mar 11;26(6). Epub 2021 Mar 11.

[email protected] Corporate Laboratory, Wilmar International Limited, Centre for Translational Medicine, 14 Medical Drive, Singapore 117599, Singapore.

Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we report a lipase detection assay which is 1000-fold more sensitive than previously reported methods. Read More

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Pancreatic fat cells of humans with type 2 diabetes display reduced adipogenic and lipolytic activity.

Am J Physiol Cell Physiol 2021 Jun 31;320(6):C1000-C1012. Epub 2021 Mar 31.

German Center for Diabetes Research (DZD e.V.), Tübingen, Germany.

Obesity, especially visceral fat accumulation, increases the risk of type 2 diabetes (T2D). The purpose of this study was to investigate the impact of T2D on the pancreatic fat depot. Pancreatic fat pads from 17 partial pancreatectomized patients (PPP) were collected, pancreatic preadipocytes isolated, and in vitro differentiated. Read More

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Optimization, purification, and biochemical characterization of thermoalkaliphilic lipase from a novel Geobacillus stearothermophilus FMR12 for detergent formulations.

Int J Biol Macromol 2021 Jun 22;181:125-135. Epub 2021 Mar 22.

Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box: 21934, Alexandria, Egypt. Electronic address:

This study was aimed to produce a high compatible thermoalkaliphilic lipase (TA) with detergents from new thermophilic bacterial strains utilizing fish wastes for industrial application. Among bacterial isolates, a new Geobacillus stearothermophilus FMR12 efficiently utilized fish wastes at a concentration of 20% (w/v), exhibiting highly lipolytic activity at extreme thermal and alkaline pH conditions. Optimized fermentation parameters of TA lipase production were ascertained, promoting the productivity of the TA lipase from 424 to 1038 U/ml. Read More

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