4,768 results match your criteria lipase production

Ternary biogenic silica/magnetite/graphene oxide composite for the hyperactivation of Candida rugosa lipase in the esterification production of ethyl valerate.

Enzyme Microb Technol 2021 Aug 24;148:109807. Epub 2021 Apr 24.

Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Johor, Malaysia; Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Johor, Malaysia.

Oil palm leaves (OPL) silica (SiO) can replace the energy-intensive, commercially produced SiO. Moreover, the agronomically sourced biogenic SiO is more biocompatible and cost-effective enzyme support, which properties could be improved by the addition of magnetite (FeO) and graphene oxide (GO) to yield better ternary support to immobilize enzymes, i.e. Read More

View Article and Full-Text PDF

Functional Annotation of circRNAs in Tea Leaves after Infection by the Tea Leaf Spot Pathogen, Lasiodiplodia theobromae.

Phytopathology 2021 Jun 10. Epub 2021 Jun 10.

Guizhou University, 71206, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Huaxi District, Guiyang, Guizhou Province of China, Guiyang, China, 550025;

Tea leaf spot, caused by Lasiodiplodia theobromae, is an important disease that can seriously decrease the production and quality of tea (Camellia sinensis [L.] Kuntze) leaves. The analysis of circular RNA (circRNA) in tea leaves after infection by the pathogen could improve understanding about the mechanism of host-pathogen interactions. Read More

View Article and Full-Text PDF

Microbial halophilic lipases: A review.

J Basic Microbiol 2021 Jun 7. Epub 2021 Jun 7.

College of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.

Microbial lipases are commercially significant due to their versatile catalytic function of hydrolysis triacylglycerol. Among these, lipases from extremophiles are optimal for industrial application. Halophilic microorganisms living in a high salinity environment, such as the ocean, salt lakes, salt wells, and so on, produce halophilic lipases. Read More

View Article and Full-Text PDF

Interactive Gene Expression Between JEF-290 and Longhorned Tick at Early Stage of Infection.

Front Physiol 2021 19;12:643389. Epub 2021 May 19.

Department of Agricultural Biology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, South Korea.

The longhorned tick, (Acari: Ixodidae), is a hard tick and a vector for severe fever with thrombocytopenia syndrome (SFTS) virus. The number of patients infected with SFTS is rapidly increasing. Recently, the invertebrate pathogen JEF-290 was reported to be useful to control the tick as an alternative to chemical acaricides, which are not easily applicable in human living areas where the tick is widely spread. Read More

View Article and Full-Text PDF

Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor.

Bioprocess Biosyst Eng 2021 Jun 5. Epub 2021 Jun 5.

Department of Chemical Engineering, School of Engineering of Lorena, University of São Paulo (USP), 12.602-810, Lorena, SP, Brazil.

This study investigated the glycerolysis of babassu oil by Burkholderia cepacia lipase immobilized on SiO-PVA particles in a continuous packed bed reactor. Experiments were conducted in a solvent-free system at 273.15 K either in an inert atmosphere or in the presence of cocoa butter to prevent lipid oxidation. Read More

View Article and Full-Text PDF

Effects of oregano essential oil as an antibiotic growth promoter alternative on growth performance, antioxidant status, and intestinal health of broilers.

Poult Sci 2021 Mar 26;100(7):101163. Epub 2021 Mar 26.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China. Electronic address:

This experiment was conducted to assess the comparative effects of dietary antibiotics and oregano essential oil (OEO) addition on growth performance, antioxidant status and intestinal health of broilers. A total of 384 one-day-old broilers were randomly allocated to 4 treatments with 6 replicates of 16 broilers each. The 4 treatments were: an antibiotic-free control diet (control), control + 20 mg/kg colistin sulfate and 20 mg/kg virginiamycin (antibiotics), control + 200 mg/kg natural oregano essential oil (NOEO), and control + 200 mg/kg synthetic oregano essential oil (SOEO). Read More

View Article and Full-Text PDF

1,3-Dioleoyl-2-palmitoyl glycerol (OPO)-Enzymatic synthesis and use as an important supplement in infant formulas.

J Food Biochem 2021 Jun 2:e13799. Epub 2021 Jun 2.

Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

1,3-dioleolyl-2-palmitate (OPO) is an important component of the human milk fat. Its unique fatty acid composition and distribution play an important role in proper infant growth and development. Owing to this, it has been attracting researchers and manufacturers to synthesize and commercialize OPO as an important human milk fat substitute added to infant formulas. Read More

View Article and Full-Text PDF

Improving the Bioremediation and in situ Production of Biocompounds of a Biodiesel-Contaminated Soil.

Environ Manage 2021 Jun 2. Epub 2021 Jun 2.

Faculty of Engineering and Architecture (FEAR), Postgraduate Program in Civil and Environmental Engineering (PPGEng), University of Passo Fundo (UPF), Passo Fundo, RS, Brazil.

We aimed to produce simultaneously biosurfactants and lipases in solid state fermentation (SSF) using Aspergillus niger, followed by the use of the fermented media on the bioremediation of oily contaminated soil, in order to valuate agro industrial residuals and reduce the contamination. The biocompounds were produced using wheat bran and corncob (80:20), 5% of soybean oil and 0.5% of sugar cane molasses in SSF for 4 d, producing 4. Read More

View Article and Full-Text PDF

Significance of two intracellular triacylglycerol lipases of Aspergillus oryzae in lipid mobilization: A perspective in industrial implication for microbial lipid production.

Gene 2021 May 30;793:145745. Epub 2021 May 30.

Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand. Electronic address:

Microbial lipid production of oleaginous strains involves in a complex cellular metabolism controlling lipid biosynthesis, accumulation and degradation. Particular storage lipid, triacylglycerol (TAG), contributes to dynamic traits of intracellular lipids and cell growth. To explore a basis of TAG degradation in the oleaginous strain of Aspergillus oryzae, the functional role of two intracellular triacylglycerol lipases, AoTgla and AoTglb, were investigated by targeted gene disruption using CRISPR/Cas9 system. Read More

View Article and Full-Text PDF

Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Promoter and Signal Peptide Shuffling System.

ACS Synth Biol 2021 Jun 2. Epub 2021 Jun 2.

Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.

Fungal peroxygenases (UPOs) have emerged as oxyfunctionalization catalysts of tremendous interest in recent years. However, their widespread use in the field of biocatalysis is still hampered by their challenging heterologous production, substantially limiting the panel of accessible enzymes for investigation and enzyme engineering. Building upon previous work on UPO production in yeast, we have developed a combined promoter and signal peptide shuffling system for episomal high throughput UPO production in the industrially relevant, methylotrophic yeast . Read More

View Article and Full-Text PDF

An overview to process design, simulation and sustainability evaluation of biodiesel production.

Biotechnol Biofuels 2021 Jun 1;14(1):129. Epub 2021 Jun 1.

Department of Chemical Engineering, Key Laboratory for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing, 100084, China.

The overwhelming concerns due to over exploitation of fossil resources necessitate the utilization of alternative energy resources. Biodiesel has been considered as one of the most adaptable alternative to fossil-derived diesel with similar properties and numerous environmental benefits. Although there are various approaches for biodiesel production, development of cost-effective and robust catalyst with efficient production methods and utilization of a variety of feedstock could be the optimum solution to bring down the production cost. Read More

View Article and Full-Text PDF

Rational Design Method Based on Techno-Economic Principles for Integration of Organic/Organic Pervaporation with Lipase Catalyzed Transesterification.

Membranes (Basel) 2021 May 28;11(6). Epub 2021 May 28.

Business Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium.

An engineering foundation is developed in this manuscript to allow the rational design of enzymatic transesterifications integrated with organic-organic pervaporation for the removal of methanol. In the first part, enzyme kinetics are elucidated for the solventless transesterification of two monoterpene alcohols with methyl acetate catalyzed by Novozym 435. Nonlinear regression revealed that three parameters (enzyme loading, forward and backward second-order reaction rate) sufficed to describe the entire conversion as a function of time. Read More

View Article and Full-Text PDF

Diversity and Oil Degradation Potential of Culturable Microbes Isolated from Chronically Contaminated Soils in Trinidad.

Microorganisms 2021 May 28;9(6). Epub 2021 May 28.

Biochemistry Research Laboratory (Rm216), Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, Trinidad and Tobago, West Indies.

Trinidad and Tobago is the largest producer of oil and natural gas in Central America and the Caribbean. Natural crude oil seeps, in addition to leaking petroleum pipelines, have resulted in chronic contamination of the surrounding terrestrial environments since the time of petroleum discovery, production, and refinement in Trinidad. In this study, we isolated microbes from soils chronically contaminated with crude oil using a culture-dependent approach with enrichment. Read More

View Article and Full-Text PDF

In Vivo Characterization and Application of the PHA Synthase from for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate.

Polymers (Basel) 2021 May 14;13(10). Epub 2021 May 14.

School of Biological Sciences, Universiti Sains Malaysia, Gelugor 11800, Malaysia.

Polyhydroxyalkanoate (PHA) is a biodegradable thermoplastic naturally synthesized by many microorganisms, and the PHA synthase (PhaC) is known to be the key enzyme involved in determining the material properties and monomer composition of the produced PHA. The ability to exploit widely distributed, commonly found soil microorganisms such as to synthesize PHA containing the lipase-degradable 4-hydroxybutyrate (4HB) monomer will allow for convenient production of biocompatible and flexible PHA. Comparisons between the wild type and mutant strains, with and without a surface layer (S-layer), respectively, in terms of gene or amino acid sequences, synthase activity, granule morphology, and PHA productivity, revealed that the S-layer is the sole factor affecting PHA biosynthesis by . Read More

View Article and Full-Text PDF

Phytochemical Profile and In Vitro Antioxidant, Antimicrobial, Vital Physiological Enzymes Inhibitory and Cytotoxic Effects of Leaves Essential Oil from Palestine.

Nidal Jaradat

Molecules 2021 May 10;26(9). Epub 2021 May 10.

Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus 00970, Palestine.

(AJ) is one of the folkloric medicinal plants and grows in the arid condition used by Palestinian Bedouins in the Al-Naqab desert for the treatment of diabetes and gastrointestinal infections. The current investigation aimed, for the first time, to characterize the (AJ) essential oil (EO) components and evaluate EO's antioxidant, anti-obesity, antidiabetic, antimicrobial, anti-inflammatory, and cytotoxic activities. The gas chromatography-mass spectrometer (GC-MS) technique was utilized to characterize the chemical ingredients of (AJ) EO, while validated biochemical approaches were utilized to evaluate the antioxidant, anti-obesity and antidiabetic. Read More

View Article and Full-Text PDF

Lipase-Catalyzed Production of Sorbitol Laurate in a "2-in-1" Deep Eutectic System: Factors Affecting the Synthesis and Scalability.

Molecules 2021 May 7;26(9). Epub 2021 May 7.

Technical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.

Surfactants, such as glycolipids, are specialty compounds that can be encountered daily in cleaning agents, pharmaceuticals or even in food. Due to their wide range of applications and, more notably, their presence in hygiene products, the demand is continuously increasing worldwide. The established chemical synthesis of glycolipids presents several disadvantages, such as lack of specificity and selectivity. Read More

View Article and Full-Text PDF

Goji berry (Lycium barbarum L.) juice reduces lifespan and premature aging of Caenorhabditis elegans: Is it safe to consume it?

Food Res Int 2021 Jun 9;144:110297. Epub 2021 Mar 9.

Federal University of Pampa (Unipampa), BR 472, Km 585, Caixa Postal 118, Uruguaiana CEP 97501-970, RS, Brazil. Electronic address:

Goji berry fruit is considered a healthy food. However, studies on its effects on aging and safety are rare. This study is the first to evaluate the effects of goji berry juice (GBJ) on oxidative stress, metabolic markers, and lifespan of Caenorhabditis elegans. Read More

View Article and Full-Text PDF

Cellular Signaling Mechanisms of Hypocretin/Orexin.

Front Neurol Neurosci 2021 28;45:91-102. Epub 2021 May 28.

Department of Pharmacology and Department of Physiology, Institute of Biomedicine, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

Orexin receptors (OXRs) are promiscuous G-protein-coupled receptors that signal via several G-proteins and, putatively, via other proteins. On which basis the signal pathways are selected and orchestrated is largely unknown. We also have an insufficient understanding of the kind of signaling that is important for specific types of cellular responses. Read More

View Article and Full-Text PDF

Proteinous pancreatic lipase inhibitor is responsible for the antiobesity effect of young barley (Hordeum vulgare L.) leaf extract.

Biosci Biotechnol Biochem 2021 May 28. Epub 2021 May 28.

Nissei Bio Co., Ltd., Megumino-kita, Eniwa, Hokkaido 061-1374, Japan.

Young barley leaves (Hordeum vulgare L.) have various health effects and are employed as an ingredient in the production of health-promoting foods. Promoting antiobesity is one such health effect; however, the mechanism and bioactive compounds are unclear. Read More

View Article and Full-Text PDF

Computational and experimental analysis on the preferential selectivity of lipases for triglycerides in Licuri oil.

Bioprocess Biosyst Eng 2021 May 26. Epub 2021 May 26.

Tiradentes University, Av. Murilo Dantas 300, Farolândia, Aracaju, SE, 49032-490, Brazil.

In the present study, we demonstrated the use of molecular docking as an efficient in silico screening tool for lipase-triglyceride interactions. Computational simulations using the crystal structures from Burkholderia cepacia lipase (BCL), Thermomyces lanuginosus lipase (TLL), and pancreatic porcine lipase (PPL) were performed to elucidate the catalytic behavior with the majority triglycerides present in Licuri oil, as follows: caprilyl-dilauryl-glycerol (CyLaLa), capryl-dilauryl-glycerol (CaLaLa), capryl-lauryl-myristoyl-glycerol (CaLaM), and dilauryl-myristoyl-glycerol (LaLaM). The computational simulation results showed that BCL has the potential to preferentially catalyze the major triglycerides present in Licuri oil, demonstrating that CyLaLa, (≈25. Read More

View Article and Full-Text PDF

Profiling Active Enzymes for Polysorbate Degradation in Biotherapeutics by Activity-Based Protein Profiling.

Anal Chem 2021 Jun 25;93(23):8161-8169. Epub 2021 May 25.

Analytical Research & Development Mass Spectrometry, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, New Jersey 07033, United States.

Polysorbate is widely used to maintain stability of biotherapeutic proteins in pharmaceutical formulation development. Degradation of polysorbate can lead to particle formation in drug products, which is a major quality concern and potential patient risk factor. Enzymatic activity from residual host cell enzymes such as lipases and esterases plays a major role for polysorbate degradation. Read More

View Article and Full-Text PDF

Increased mRNA stability and expression level of lipase gene developed through molecular evolution process.

J Microbiol Biotechnol 2021 May 19;31(6). Epub 2021 May 19.

Division of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Republic of Korea.

In order to use an enzyme industrially, it is necessary to increase the activity of the enzyme and optimize the reaction characteristics through molecular evolution techniques. We used the error-prone PCR method to improve the reaction characteristics of LipCA lipase discovered in Antarctic . Recombinant colonies showing large halo zones were selected in tributyrin-containing medium. Read More

View Article and Full-Text PDF

Distal Pancreatic Resection with Splenectomy in the Rat: A Pancreatic Fistula Model to Investigate Postsurgical Damage?

Eur Surg Res 2021 May 21:1-8. Epub 2021 May 21.

Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Center of Operative Medicine, Medical University of Innsbruck, Innsbruck, Austria.

Background: Postoperative pancreatic fistula (POPF) is a major complication in pancreatic surgery and can cause considerable postoperative morbidity. Advanced surgical-technical approaches to prevent POPF did not yield a substantial improvement. To investigate innovative treatments, experimental animal models of distal pancreatic resection and pancreaticoduodenectomy are of fundamental importance. Read More

View Article and Full-Text PDF

Production of a fermented solid containing lipases from Penicillium roqueforti ATCC 10110 and its direct employment in organic medium in ethyl oleate synthesis.

Biotechnol Appl Biochem 2021 May 22. Epub 2021 May 22.

Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Brazil.

The production and direct employment in organic medium in the ethyl-oleate synthesis of a fermented solid (FS) containing lipases by Penicillium roqueforti ATCC 10110 (PR10110) was investigated. For the production of this FS, the solid-state fermentation of different agroindustrial waste was used, such as: cocoa shell, sugarcane bagasse, sugarcane bagasse with cocoa shell, and cocoa shell with soybean oil and nutrient solution. The response surface methodology was used to study the effect of independent variables of initial moisture content and inductor concentration, as carbon source and inducer on lipase production. Read More

View Article and Full-Text PDF

Effects of temperature, dissolved oxygen, and their interaction on the growth performance and condition of rainbow trout (Oncorhynchus mykiss).

J Therm Biol 2021 May 3;98:102928. Epub 2021 Apr 3.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, Shandong Province, 266100, China.

The individual effects of temperature and dissolved oxygen (DO) on rainbow trout (Oncorhynchus mykiss), an important aquaculture species, are clearly established; however, little is known about the interactive effects of these parameters. In this study, the effects of temperature, DO, and their interaction on the growth, antioxidant status, digestive enzyme activity, serum biochemical parameters, and liver IGF-1 expression in rainbow trout were evaluated. Fish (initial weight, 109. Read More

View Article and Full-Text PDF

Recombinant Protein Production and Purification Using Eukaryotic Cell Factories.

Asmaa Missoum

Methods Mol Biol 2021 ;2290:215-228

Paris-Saclay University, Orsay, France.

Cloning proteins enables their production and characterization for further studies. This requires inserting the gene of the studied protein to be inserted in a vector, which then will be transformed to the host cell used as "factory." Consequently, the "biomass" of host cells will be produced using bioreactors. Read More

View Article and Full-Text PDF
January 2021

Triglyceride metabolism and classification of hypertriglyceridemias.

Clin Investig Arterioscler 2021 May;33 Suppl 2:1-6

Unitat de Medicina Vascular i Metabolisme, Hospital Universitari Sant Joan, Reus, Tarragona, España; Unitat de Reçerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, IISPV, Reus, Tarragona, España; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid, España.

Triglycerides (TG) are the most important molecules for the energy reserve of our body. After their hepatic or intestinal synthesis from fatty acids, they are carried by chylomicrons (QM (intestinal origin) or VLDL (hepatic origin) in plasma. Their catabolism is determined by the action of the lipoprotein lipase protein complex (LPL) and the hepatic receptors (RLDL and LRP-1) are responsible for their clearance are. Read More

View Article and Full-Text PDF

Sex-dependent changes in the microbiota profile, serum metabolism, and hormone levels of growing pigs after dietary supplementation with Lactobacillus.

Appl Microbiol Biotechnol 2021 Jun 18;105(11):4775-4789. Epub 2021 May 18.

Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Dietary supplementation with Lactobacillus can improve the intestinal microbial balance and exerts beneficial effects on pig health. However, whether these effects in pigs show differences between the sexes remains poorly understood. Therefore, we analyzed the sex-dependent patterns in the fecal microbiota after dietary supplementation with Lactobacillus acidophilus ZLA012 through high-throughput sequencing, determined the metabolomic profile of serum in barrows (immature castrated males) and gilts (sexually immature females) through nontargeted liquid chromatography-mass spectrometry, and measured the levels of various hormones, such as insulin, growth hormone, serotonin, triiodothyronine, and thyroxine. Read More

View Article and Full-Text PDF

The Effect of Lactococcus lactis subsp. lactis PTCC 1403 on the Growth Performance, Digestive Enzymes Activity, Antioxidative Status, Immune Response, and Disease Resistance of Rainbow Trout (Oncorhynchus mykiss).

Probiotics Antimicrob Proteins 2021 May 18. Epub 2021 May 18.

Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.

The effect of Lactococcus lactis subsp. lactis strain PTCC 1403 as a potential probiotic was investigated on the growth, hematobiochemical, immune responses, and resistance to Yersinia ruckeri infection in rainbow trout. A total of 240 fish were distributed into 12 fiberglass tanks representing four groups (× 3 replicates). Read More

View Article and Full-Text PDF

A novel anti-infective molecule nesfactin identified from sponge associated bacteria Nesterenkonia sp. MSA31 against multidrug resistant Pseudomonas aeruginosa.

Microb Pathog 2021 May 14;157:104923. Epub 2021 May 14.

Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India. Electronic address:

Overuse of antibiotics coupled with biofilm-forming ability has led to the emergence of multi-drug P. aeruginosa strains worldwide. Quorum sensing is a bacterial cell-cell communication system that regulates the expression of genes, including virulence factors, through production of acyl-homoserine lactones (AHLs) in Pseudomonas aeruginosa. Read More

View Article and Full-Text PDF