203,610 results match your criteria linked immunosorbent


Georgian Med News 2021 Mar(312):42-46

Bogomolets National Medical University, Institute of Postgraduate Education, Department of Dentistry, Kyiv, Ukraine.

In recent years, according to the WHO, more than 1,9 billion people over the age of 18 are overweight, of whom more than 650 million are obese, with an annual trend. Studies in recent decades have shown that obese young people with generalized periodontitis have abnormalities in protein, fat and carbohydrate metabolism, which contributes to the early destruction of the periodontal tissue complex and rapid tooth loss. The aim of our study was to examine changes in serum leptin and adiponectin in patients with generalized periodontitis on the background of obesity. Read More

View Article and Full-Text PDF

Effects of a combination of Mass and Dutch variant as an inactivated vaccine against variant 2 avian infectious bronchitis virus challenge.

Microb Pathog 2021 May 5:104937. Epub 2021 May 5.

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

The present study aimed to evaluate the effects of inactivated vaccines combining Mass and Dutch variants as vaccine boosters after H120 priming on inhibiting variant 2 viral load in the kidneys (as the target organ) and reducing fecal shedding. Ciliostasis score and antibody response were investigated as well. A total of 150 specific-pathogen-free (SPF) chicken were divided into six groups. Read More

View Article and Full-Text PDF

Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis.

Gene 2021 May 5:145699. Epub 2021 May 5.

Animal Science and Technology College, Gansu Agricultural University, Lanzhou, China. Electronic address:

Progesterone (P4) is an anti-androgen compound whose role in sperm maturation and functionality remains unclear in sheep. Here, we aimed to investigate the regulation mechanism of P4 on the epididymal secretion of dihydrotestosterone (DHT). To this end, we performed enzyme-linked immunosorbent assays, immunohistochemical staining, western blotting, and quantitative real-time polymerase chain reaction to detect P4 concentration as well as StAR, P450scc, and 3β-HSD expression in sheep epididymis. Read More

View Article and Full-Text PDF

Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice.

Acta Biochim Biophys Sin (Shanghai) 2021 May 8. Epub 2021 May 8.

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

The major etiologic agent that causes acute gastroenteritis worldwide in young animals and children is Group A rotavirus. Currently, commercially available vaccines do not often prevent porcine rotavirus (PRV) infection. In this study, we evaluated the efficacy of oral recombinant Lactobacillus vaccine against PRV in a mouse model. Read More

View Article and Full-Text PDF

Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers.

Sci Rep 2021 May 7;11(1):9808. Epub 2021 May 7.

Department of Plastics Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA, 01854, USA.

Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). Read More

View Article and Full-Text PDF

An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay.

Immunohematology 2021 Mar;37(1):33-43

Center for Transfusion Medicine and Cellular Therapies, and Department of Pathology and Laboratory Medicine, Emory University School of Medicine , 201 Dowman Drive, Atlanta, GA 30322 , and Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School , 630E New Research Building, 77 Avenue Louis Pasteur, Boston, MA 02115.

While a variety of therapeutic options continue to emerge for COVID-19 treatment, convalescent plasma (CP) has been used as a possible treatment option early in the pandemic. One of the most significant challenges with CP therapy, however, both when defining its efficacy and implementing its approach clinically, is accurately and efficiently characterizing an otherwise heterogenous therapeutic treatment. Given current limitations, our goal is to leverage a SARS antibody testing platform with a newly developed automated endpoint titer analysis program to rapidly define SARS-CoV-2 antibody levels in CP donors and hospitalized patients. Read More

View Article and Full-Text PDF

Combining urine antigen and blood polymerase chain reaction for the diagnosis of disseminated histoplasmosis in hospitalized patients with advanced HIV disease.

Med Mycol 2021 May 7. Epub 2021 May 7.

Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, São Paulo 01246-000, Brazil.

Disseminated histoplasmosis (DH) is endemic in Latin America and the Caribbean where diagnostic tools are restricted. We carried-out a 1-year prospective cohort study at a referral hospital in São Paulo, Brazil. Participants had > or =18 years old, were hospitalized due to any indication and had CD4+ < 200 cells/µl. Read More

View Article and Full-Text PDF

Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium.

Arch Oral Biol 2021 Apr 24;127:105134. Epub 2021 Apr 24.

Department of Physiology and Pathology, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP, Brazil.

Objective: This study aimed to assess the influence of the bisphosphonates zoledronic acid and sodium alendronate on MMP-2 and MMP-9 synthesis and activity by gingival fibroblasts seeded onto titanium substrate.

Design: Titanium discs were placed in 24-well cell culture plates and gingival fibroblasts were seeded (1 × 10 cells/discs) on them using Dulbecco's Modified Eagle's Medium (DMEM) + 10 % fetal bovine serum (FBS) for 24 h. After this period, a fresh serum-free DMEM containing zoledronic acid or sodium alendronate at 0. Read More

View Article and Full-Text PDF

Establishment of a novel double-monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA): Tool for human B7-H4 detection in autoimmune diseases.

Clin Exp Immunol 2021 May 7. Epub 2021 May 7.

Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, China.

B7-H4, one of the immunoregulatory proteins, plays an inhibitory role by inhibiting T cell proliferation and cytokine production. Nevertheless, the significance of soluble B7-H4 (sB7-H4) in autoimmune diseases is unclear. In our study, we developed two novel mouse anti-human B7-H4 monoclonal antibodies (mAbs) (clone 8D4 and 7E1) with utilities for flow cytometry, immunoblotting and immunofluorescence. Read More

View Article and Full-Text PDF

Effects of the Maillard reaction on the epitopes and immunoreactivity of tropomyosin, a major allergen in .

Food Funct 2021 May 7. Epub 2021 May 7.

College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Scallop (Chlamys nobilis) causes an IgE-mediated food allergy; however, studies of the allergens in its musculus are not sufficiently comprehensive. In this context, the target protein was purified from scallops and confirmed to be the major allergen tropomyosin (TM) using proteomic technology and serological testing. Subsequently, seven potential IgE epitopes of TM were obtained using phage display technology with IgE enrichment from the serum of scallop-sensitized patients and identified via inhibition enzyme-linked immunosorbent assays. Read More

View Article and Full-Text PDF

Clinicians' pearls and myths in pemphigus.

Ital J Dermatol Venerol 2021 Apr;156(2):142-146

Department of Dermatology, Center for Blistering Diseases, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.

Pemphigus comprises a heterogeneous group of autoimmune blistering diseases, which can affect both skin and mucous membranes, especially oral mucosa. This group of diseases shows usually a chronic-relapsing course. Since pemphigus is a rare disease, the diagnosis is often delayed, because it is based upon the recognition of consistent clinical, histologic, and direct immunofluorescence findings, as well as indirect immunofluorescence, and/or enzyme-linked immunosorbent assay. Read More

View Article and Full-Text PDF

Cord blood levels of interleukin-10 decrease in neonates with increased birth weight: novel implications of the cytokine network in early obesity.

Eur J Pediatr 2021 May 7. Epub 2021 May 7.

Laboratory of Immunometabolism, Research Division, General Hospital of Mexico "Dr. Eduardo Liceaga", 06720, Mexico City, Mexico.

Interleukin-10 (IL-10) and interferon-gamma (IFN-gamma) are associated with body weight alterations in children, adolescents, and adults. However, little is known regarding the role of IL-10 and IFN-gamma in birth weight of neonates. One hundred eighty-two infants were enrolled and divided in groups of normal birth weight (< 95th percentile) or increased birth weight (> 95th percentile) for gestational age. Read More

View Article and Full-Text PDF

Evidence for Transmission of Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda.

Front Vet Sci 2021 20;8:645076. Epub 2021 Apr 20.

Área de Parasitología del Departamento de Agroquímica y Medioambiente, Universidad Miguel Hernández de Elche, Alicante, Spain.

Cysticercosis is a parasitic infection caused by the metacestode larval stage (cysticercus) of . In humans, cysticercosis may infect the central nervous system and cause neurocysticercosis, which is responsible for over 50,000 deaths per year worldwide and is the major cause of preventable epilepsy cases, especially in low-income countries. Cysticercosis infection is endemic in many less developed countries where poor hygiene conditions and free-range pig management favor their transmission. Read More

View Article and Full-Text PDF

The Prevalence of Hepatitis B Virus Markers among Students of Shiraz University of Medical Sciences.

Adv Biomed Res 2021 26;10. Epub 2021 Feb 26.

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Protection against hepatitis B virus (HBV) is based on the presence of antibodies against hepatitis B surface antigen (HBsAg). Vaccination of newborns is the most effective means of prevention. This study aimed to evaluate the frequency of anti-HBs antibody (anti-HBsAb), anti-HB core Ab (anti-HBcAb), HBsAg, and HBV DNA among university students in Fars province, Southern Iran. Read More

View Article and Full-Text PDF
February 2021

The Circulating Midkine in the Newly Diagnosed Celiac Disease: Clinical Implications.

Adv Biomed Res 2021 27;10. Epub 2021 Jan 27.

Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Celiac disease (CeD) is a chronic inflammatory small intestine disorder caused by an abnormal immune response to an array of the epitopes of the wheat gluten and related proteins of rye and barley in genetically susceptible individuals. Midkine (MK) is an angiogenic cytokine, chemotactic in the direction of polymorphonuclear neutrophils and macrophages, and a T-regulatory cell suppressor. So far, a possible relationship with CeD has not yet been explored. Read More

View Article and Full-Text PDF
January 2021

Arousing Effects of Electroacupuncture on the "Shuigou Point" in Rats with Disorder of Consciousness after Traumatic Brain Injury.

Evid Based Complement Alternat Med 2021 19;2021:6611461. Epub 2021 Apr 19.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Orexin is an important neuropeptide that stimulates cortical activation and arousal and is involved in the regulation of wakefulness and arousal. Our previous meta-analysis showed that acupuncture fared well in the treatment of TBI-induced DOC in which "shuigou (DU 26)" was the most important and frequent point targeted. In the present study, we investigated whether electroacupuncture (EA) promotes TBI-induced unconsciousness wakefulness via orexin pathway. Read More

View Article and Full-Text PDF

Soluble BTN2A1 Is a Potential Prognosis Biomarker in Pre-Treated Advanced Renal Cell Carcinoma.

Front Immunol 2021 20;12:670827. Epub 2021 Apr 20.

Team Immunity and Cancer, Centre de Recherche en Cancérologie de Marseille (CRCM), Inserm, U1068, CNRS, UMR7258, Institut Paoli-Calmettes, Aix-Marseille University, UM105, Marseille, France.

The development of immune checkpoint inhibitors (ICI) has dramatically changed the landscape of therapies for metastatic renal cell carcinoma. However, many patients do not benefit from such therapy and prognostic or predictive validated biomarker validated for ICI are still needed to better select and treat patient. Plasmatic soluble immune checkpoints have been described as potential immune biomarkers in hematological malignancies and solids tumors, then, we would like to explore the prognostic value of different soluble immune checkpoints in patients with mRCC treated with nivolumab after TKI. Read More

View Article and Full-Text PDF

Development of Vasoinhibin-Specific Monoclonal Antibodies.

Front Endocrinol (Lausanne) 2021 20;12:645085. Epub 2021 Apr 20.

Institute for Clinical Chemistry, Laboratory Medicine and Transfusion Medicine, Nuremberg General Hospital & Paracelsus Medical University, Nuremberg, Germany.

Vasoinhibin is a protein hormone with antiangiogenic, antivasodilatatory, and antivasopermeability effects generated by the proteolytic cleavage of prolactin. The discovery of its role in diabetic retinopathy and peripartum cardiomyopathy led to the evaluation of new pharmacological treatments in clinical interventional trials. However, the quantitative evaluation of vasoinhibin in biological samples from patients has not been possible due to the lack of vasoinhibin-specific antibodies. Read More

View Article and Full-Text PDF

The Predictive Value of Myeloperoxidase for Contrast-Induced Nephropathy After Percutaneous Coronary Intervention in Patients with Acute Myocardial Infarction.

Int J Gen Med 2021 30;14:1621-1629. Epub 2021 Apr 30.

Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, 210009, People's Republic of China.

Background: Higher serum myeloperoxidase (MPO) in patients with acute coronary syndrome is associated with adverse cardiovascular outcomes. Contrast-induced nephropathy (CIN) is associated with worse prognosis in patients with coronary artery disease following angiography. We have no idea whether patients with higher serum myeloperoxidase have a higher risk of CIN in acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Read More

View Article and Full-Text PDF

Rotavirus gastroenteritis in Pakistan, 2018: updated disease burden.

BMC Infect Dis 2021 May 6;21(1):426. Epub 2021 May 6.

Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.

Objective: Rotavirus A (RVA) is a significant cause of severe diarrheal illness and one of the common causes of death in children under the age of five. This study was aimed at detecting the prevalence of RVA in Pakistan after rotavirus vaccines were introduced. Fecal samples were obtained from 813 children from different hospitals in Rawalpindi and Islamabad, Pakistan, from January 2018 to December 2018. Read More

View Article and Full-Text PDF

Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth.

BMC Immunol 2021 May 6;22(1):28. Epub 2021 May 6.

Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Polo III - Health Sciences Campus, Azinhaga Santa Comba, Celas, 3000-548, Coimbra, Portugal.

Background: Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveries. Read More

View Article and Full-Text PDF

A biolabel research based on metabonomics reveals the therapeutic potentials of Herba Lysimachiae in synovial diseases: The dual effects on synovial platelet aggregation by prostaglandin E1/E2.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Apr 20;1174:122726. Epub 2021 Apr 20.

The Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161006, PR China.

Previous research has demonstrated that Herba Lysimachiae (HL) exerts the dual effects on platelet aggregation in the synovium, which may contribute to its protection against synovial lesions under different situations. However, the mechanism is unclear. In the present experiment, a biolabel research based on metabonomics was used to mine the information about the intervention of HL on synovium at the metabolite level, which may help to analyze the regulation of HL on synovial platelet aggregation and its possible treatment in synovial diseases. Read More

View Article and Full-Text PDF

Alarmins (IL-33, sST2, HMGB1, and S100B) as potential biomarkers for schizophrenia.

J Psychiatr Res 2021 Apr 29;138:380-387. Epub 2021 Apr 29.

Department of Experimental Immunology, Medical University of Lodz, Lodz, Poland.

There is growing evidence that immune/inflammatory processes are related to the etiology of schizophrenia. Danger-/damage-associated molecular patterns (DAMPs), also called alarmins, are recognized as inflammatory mediators that play an important role in the development of many infection-induced or sterile inflammatory diseases. The importance of DAMPs particles in various mental disorders is still not clear. Read More

View Article and Full-Text PDF

Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker.

Anal Biochem 2021 May 3:114215. Epub 2021 May 3.

Universidade Federal Do Pampa, Campus Caçapava Do Sul, RS, Brazil. Electronic address:

Immunoassays have been extensively applied in the medical diagnostic field. Enzyme-Linked Immunosorbent Assay (ELISA) and Lateral Flow Immunochemical Assay (LFIA) are methods that have been well established to analysis of clinical substances such as protein, hormones, drugs, identification of antibodies and in the quantification of antigen. Over the past years, the application of these methods has been extended to assess the clinical oxidative stress condition based on monitoring of the 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) biomarker levels. Read More

View Article and Full-Text PDF

A longitudinal study of antibody responses to selected host antigens in rheumatic fever and rheumatic heart disease.

J Med Microbiol 2021 May;70(5)

Department of Microbiology, Seth G S Medical College and King Edward Memorial Hospital, Mumbai, India.

Group A streptococci can trigger autoimmune responses that lead to acute rheumatic fever (ARF) and rheumatic heart disease (RHD). Some autoantibodies generated in ARF/RHD target antigens in the S2 subfragment region of cardiac myosin. However, little is known about the kinetics of these antibodies during the disease process. Read More

View Article and Full-Text PDF

Can renalase be a novel candidate biomarker for distinguishing renal tumors?

Biotech Histochem 2020 Oct 14:1-6. Epub 2020 Oct 14.

Department of Medical Biochemistry and Clinical Biochemistry, Firat Hormones Research Group, School of Medicine, Firat University Elazig, Elazig, Turkey.

Renalase (RNLS) is synthesized mainly in renal tissues. The function of RNLS in cancerous renal tissues has not been investigated. We investigated the synthesis of RNLS in chromophobe renal cell carcinoma, papillary renal cell carcinoma and clear cell renal cell carcinoma with Fuhrman grades (FG): FG1, nucleoli are absent or inconspicuous and basophilic; FG2, nucleoli are conspicuous and eosinophilic and visible but not prominent; FG3, nucleoli are conspicuous and eosinophilic; FG4, extreme nuclear pleomorphism, multinucleate giant cells, and/or rhabdoid and/or sarcomatoid differentiation. Read More

View Article and Full-Text PDF
October 2020

Numerical Modeling for Sensitive and Rapid Molecular Detection by Membrane-Based Immunosensors.

Anal Chem 2021 May 6. Epub 2021 May 6.

Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.

Rapid, simple, and sensitive point-of-care testing (POCT) has attracted attention in recent years due to its excellent potential for early disease diagnosis and health monitoring. The flow-through biosensor design is a candidate for POCT that utilizes the small-sized pores of a porous membrane as a recognition space where it emits a signal comparable to that of a conventional enzyme-linked immunosorbent assay within 35 min of detection time. In this paper, we present a numerical model for this immunosensing technology to systematically design an improved recognition system. Read More

View Article and Full-Text PDF

Detection and inhibition of IgE antibodies reactive with cross-reactive carbohydrate determinants in an ELISA for allergen-specific IgE in horses.

Vet Dermatol 2021 May 6. Epub 2021 May 6.

Montesano & Tallarico, LLP, PO Box 1396, Smithtown, NY, USA.

Background: It has been demonstrated that immunoglobulin (Ig)E specific for cross-reactive carbohydrate determinants (CCD) is present in the serum of sensitized humans, dogs and cats, and that these CCD-specific antibodies might confound serological testing.

Hypothesis/objective: The objective was to determine whether or not CCD-reactive antibodies occur in horses and to investigate the prevalence of CCD-reactive IgE antibodies in equine sera using a monoclonal cocktail-based enzyme-linked immunosorbent assay designed to detect allergen-specific IgE in horses, and to evaluate a means for successful inhibition of these CCD.

Methods And Materials: Sera from 28 horses suspected of clinical allergy were evaluated, with and without a proprietary inhibitor which contains carbohydrates derived from bromelain (BROM-CCD), using a panel of 72 allergens that include 15 grasses, 17 trees, nine weeds, five mites, 12 fungi, 12 insects and two environmental allergens. Read More

View Article and Full-Text PDF

Simultaneous determination of ochratoxin A and enterotoxin A in milk by magnetic nanoparticles based fluorescent immunoassay.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 May 6:1-19. Epub 2021 May 6.

Laboratory of Experimental Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

Ochratoxin A (OTA) and staphylococcus enterotoxin A (SEA) are highly toxic contaminants and have induced human health problems. They commonly occur in milk and milk products. A competitive fluorescent immunoassay was developed for rapid and simultaneous determination of these toxins in milk samples. Read More

View Article and Full-Text PDF

Seroprevalence of Infection in Patients With Chronic Obstructive Pulmonary Disease in England: Analysis of the AERIS Cohort.

COPD 2021 May 6:1-14. Epub 2021 May 6.

GSK, Wavre, Belgium.

Pertussis is underdiagnosed and underreported in adults and patients with underlying conditions. Patients with chronic obstructive pulmonary disease (COPD) may be at increased risk of severe pertussis. Understanding the true prevalence of pertussis infections in such patients is important. Read More

View Article and Full-Text PDF