6,388 results match your criteria levels zebrafish


A reversible coumarin-based sensor for intracellular monitoring cysteine level changes during Cu-induced redox imbalance.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 14;263:120173. Epub 2021 Jul 14.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

Biological thiols are crucial small molecule amino acids widely existing in cells, which play indispensable roles in maintaining redox homeostasis of living systems. Owing to their abnormal levels have close relation with many diseases, thus, developing more convenient, rapid and practical in-vivo detection tools is imminent. Herein, a reversible coumarin-based probe (HNA) was successfully constructed through a simple two-step synthesis. Read More

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Effects of Sex and Group Size on Behavior and Brain Biogenic Amines in Short-Lived Turquoise Killifish ().

Zebrafish 2021 Jul 29. Epub 2021 Jul 29.

Federal Research Center Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.

Short-lived turquoise killifish () becomes a popular model species for neuroscience. However, the effects of sex and rearing conditions on behavior and brain monoamines in are unknown. In this article, we study the body mass, behavior in the novel tank diving test, levels of noradrenaline (NA), dopamine (DA), serotonin (5-HT), 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) in the brain of 108 day-old males and females reared in small (one male and one or two females in 4-L tanks) and large (four males and four females in 25-L tanks) groups. Read More

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PAH SORPTION TO NANOPLASTICS AND THE TROJAN HORSE EFFECT AS DRIVERS OF MITOCHONDRIAL TOXICITY AND PAH LOCALIZATION IN ZEBRAFISH.

Front Environ Sci 2020 Jul 24;8. Epub 2020 Jul 24.

Nicholas School of the Environment, Duke University, Durham, NC, USA.

Plastics are world-wide pollutants that pose a potential threat to wildlife and human health. Small plastic particles, such as microplastics and nanoplastics, are easily ingested, and can act as a Trojan Horse by carrying microorganisms and pollutants. This study investigated the potential role of the Trojan Horse effect in the toxicity of nanoplastics to the vertebrate model organism, zebrafish (Danio rerio). Read More

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Role of the cardiovascular system in ammonia excretion in early life stages of zebrafish ().

Am J Physiol Regul Integr Comp Physiol 2021 07 28. Epub 2021 Jul 28.

Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL, United States.

The purpose of this study was to investigate if the cardiovascular system is important for ammonia excretion in the early life stages of zebrafish. Morpholino knockdowns of cardiac troponin T (TNNT2) or vascular endothelial growth factor (VEGFA) provided morphants with non-functional circulation. At the embryonic stage (30-36 hpf), ammonia excretion was not constrained by lack of cardiovascular function. Read More

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Knockdown screening of chromatin binding and regulatory proteins in zebrafish identified Suz12b as a regulator of tfpia and an antithrombotic drug target.

Sci Rep 2021 Jul 27;11(1):15238. Epub 2021 Jul 27.

Department of Biological Sciences, University of North Texas, 1511 West Sycamore Street, Denton, TX, 76203, USA.

Tissue factor pathway inhibitor (TFPI) is an anticoagulant protein that inhibits factor VIIa and Xa in the coagulation cascade. It has been shown that forkhead box P3 protein is a TFPI transcriptional repressor. However, there are no studies on chromatin remodeling that control TFPI expression. Read More

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The anti-melanogenic effects of 3-O-ethyl ascorbic acid via Nrf2-mediated α-MSH inhibition in UVA-irradiated keratinocytes and autophagy induction in melanocytes.

Free Radic Biol Med 2021 Jul 24. Epub 2021 Jul 24.

Institute of Nutrition, College of Health Care, China Medical University, Taichung, 406040, Taiwan. Electronic address:

3-O-ethyl ascorbic acid (EAA) is an ether-derivative of ascorbic acid, known to inhibit tyrosinase activity, and is widely used in skincare formulations. Nevertheless, the molecular mechanisms underlying the EAA's effects are poorly understood. Here, the anti-melanogenic activity of EAA was demonstrated through Nrf2-mediated α-MSH inhibition in UVA-irradiated keratinocytes (HaCaT) and autophagy induction and inhibition of α-MSH-stimulated melanogenesis in melanocytes (B16F10). Read More

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NV14 from serine O-acetyltransferase of cyanobacteria influences the antioxidant enzymes in vitro cells, gene expression against H O and other responses in vivo zebrafish larval model.

Cell Biol Int 2021 Jul 27. Epub 2021 Jul 27.

SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603 203, Chennai, Tamil Nadu, India.

In this study, we have identified a novel peptide NV14 with anti-oxidative functions from serine O-acetyltransferase (SAT) of Artrospira platensis (Ap). The full sequence of ApSAT and its derived NV14 peptide 'NVRIGAGSVVLRDV' (141-154) was characterized using bioinformatics tools. To address the transcriptional activity of ApSAT in response to induce generic oxidative stress, the spirulina culture was exposed to H O (10mM). Read More

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Propolis Can Improve Caudal Fin Regeneration in Zebrafish () Induced by The Combined Administration of Alloxan and Glucose.

Zebrafish 2021 Jul 23. Epub 2021 Jul 23.

Parasitology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

Hyperglycemia, a primary symptom in diabetes mellitus, is associated with difficulties in wound healing and regeneration. This condition is due to the length of the inflammatory phase and free radicals. Furthermore, there is evidence that molecular pathogenesis is involved in impaired wound healing in diabetics. Read More

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Developmental phenotypic and transcriptomic effects of exposure to nanomolar levels of metformin in zebrafish.

Environ Toxicol Pharmacol 2021 Jul 24;87:103716. Epub 2021 Jul 24.

Institute of Environmental Health Sciences, Wayne State University, 6135 Woodward Ave, Detroit, MI, 48202, USA; Department of Pharmacology, Wayne State University, 540 E Canfield, Detroit, MI, 28201, USA. Electronic address:

Metformin is found in the majority of lakes and streams in the United States, leading to widespread environmental exposure. Results of the present study indicate that extended duration metformin exposure at critical developmental periods leads to decreased survival rates in zebrafish (danio rerio), an NIH approved human model. Significant abnormalities are seen with extended duration metformin exposure from 4 h post fertilization up to 5 days post fertilization, although short term metformin exposure for 24 h at 4-5 days post fertilization did not lead to any significant abnormalities. Read More

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Zebrafish as a new model for rhododendrol-induced leukoderma.

Pigment Cell Melanoma Res 2021 Jul 26. Epub 2021 Jul 26.

Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan.

Idiopathic leukoderma is a skin disorder characterized by patchy loss of skin pigmentation due to melanocyte dysfunction or deficiency. Rhododendrol (RD) was approved as a cosmetic ingredient in Japan in 2008. However, it was shown to induce leukoderma in approximately 20,000 customers. Read More

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Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio).

Neurochem Res 2021 Jul 26. Epub 2021 Jul 26.

Programa de Pós-Graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brazil.

Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Read More

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Joint toxicity of lead and cadmium on the behavior of zebrafish larvae: An antagonism.

Aquat Toxicol 2021 Jul 16;238:105912. Epub 2021 Jul 16.

Department of Occupational Health and Occupational Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, China. Electronic address:

Although the individual toxicity of lead (Pb) and cadmium (Cd) was intensively studied, little is known about their joint toxicity on the development of circadian behavioral rhythm. Therefore, we co-exposed zebrafish to Pb and Cd to investigate the alterations of behavioral rhythm and the potential mechanism. Inductively coupled plasma mass spectrometry analysis was used to detect the internal exposure level of heavy metals. Read More

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A novel zebrafish model for intermediate type spinal muscular atrophy demonstrates importance of Smn for maintenance of mature motor neurons.

Hum Mol Genet 2021 Jul 23. Epub 2021 Jul 23.

Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, Singapore 117543, Singapore.

A deficiency in Survival Motor Neuron (SMN) protein results in motor neuron loss in spinal muscular atrophy (SMA) patients. Human SMN is encoded by SMN1 and SMN2 that differ by a single C6T transition in a splice regulatory region of exon 7. In SMN2, exon 7 is skipped leading to an unstable protein, which cannot compensate for SMN1 loss in SMA patients. Read More

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A connexin/ifi30 pathway bridges HSCs with their niche to dampen oxidative stress.

Nat Commun 2021 07 23;12(1):4484. Epub 2021 Jul 23.

University of Geneva, Faculty of Medicine, Department of Pathology and Immunology, Geneva 4, Switzerland.

Reactive oxygen species (ROS) represent a by-product of metabolism and their excess is toxic for hematopoietic stem and progenitor cells (HSPCs). During embryogenesis, a small number of HSPCs are produced from the hemogenic endothelium, before they colonize a transient organ where they expand, for example the fetal liver in mammals. In this study, we use zebrafish to understand the molecular mechanisms that are important in the caudal hematopoietic tissue (equivalent to the mammalian fetal liver) to promote HSPC expansion. Read More

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Study on the Joint Toxicity of BPZ, BPS, BPC and BPF to Zebrafish.

Molecules 2021 Jul 9;26(14). Epub 2021 Jul 9.

School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China.

Bisphenol Z (BPZ), bisphenol S (BPS), bisphenol C (BPC), and bisphenol F (BPF) had been widely used as alternatives to bisphenol A (BPA), but the toxicity data of these bisphenol analogues were very limited. In this study, the joint toxicity of BPZ, BPS, BPC, and BPF to zebrafish () was investigated. The median half lethal concentrations (LC50) of BPZ, BPS, BPC, and BPF to zebrafish for 96 h were 6. Read More

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Flavonoid Glycosides from var. (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis.

Int J Mol Sci 2021 Jul 19;22(14). Epub 2021 Jul 19.

Department of Marine Life Science, Jeju National University, Jeju 63243, Korea.

extracts possess a broad spectrum of biological activities, such as antioxidant and anticancer activities in melanoma cancers. Nevertheless, the compounds contain high antioxidant capacities and anticancer activities in melanoma cells, shown to be effective in hyperpigmentation disorders, but whether flavonoid glycosides from . regulate anti-melanogenesis remains unclear. Read More

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Oxytocin Signaling Acts as a Marker for Environmental Stressors in Zebrafish.

Int J Mol Sci 2021 Jul 12;22(14). Epub 2021 Jul 12.

Department of Life Science, National Taiwan University, Taipei 10617, Taiwan.

The oxytocin system plays a role in stress responses and behavior modulation. However, the effects of oxytocin signaling on stress adaptation remain unclear. Here, we demonstrated the roles of oxytocin signaling as a biomarker under stress conditions in the peripheral tissues (the gills) and central nervous system (the brain). Read More

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Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser-induced pigment ablation.

Lasers Surg Med 2021 Jul 23. Epub 2021 Jul 23.

Department of Dermatology, College of Medicine, Korea University, Seoul, Republic of Korea.

Background And Objectives: Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post-inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. Read More

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Altered redox homeostasis in steroid-depleted follicles attenuates hCG regulation of follicular events: Cross-talk between endocrine and IGF axis in maturing oocytes.

Free Radic Biol Med 2021 Jul 18;172:675-687. Epub 2021 Jul 18.

Molecular and Cellular Endocrinology Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, 731235, India. Electronic address:

Steroids and insulin-like growth factors (Igfs) are indispensable for folliculogenesis and reproductive fitness in the vertebrate ovary. The intrafollicular redox balance is also of immense importance for ovarian follicles wherein low levels of ROS are being utilized for cell signalling and regulation of gene expression; its excess may interfere with normal physiological processes leading to ovotoxicity. However, the functional relevance of ovarian steroidogenesis in maintaining the follicular microenvironment with coordinated redox homeostasis and intra-ovarian growth factors axis is relatively less understood. Read More

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Hif-1α is not required for the development of cardiac adrenergic control in zebrafish (Danio rerio).

J Exp Zool A Ecol Integr Physiol 2021 Aug;335(7):623-631

Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.

Adrenergic regulation, acting via the sympathetic nervous system, provides a major mechanism to control cardiac function. It has recently been shown that hypoxia inducible factor-1α (Hif-1α) is necessary for normal development of sympathetic innervation and control of cardiac function in the mouse. To investigate whether this may represent a fundamental trait shared across vertebrates, we assessed adrenergic regulation of the heart in wild-type and Hif-1α knockout (hif-1α ) zebrafish (Danio rerio). Read More

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Sublethal Effect Concentrations for Non-Polar Narcosis in the Zebrafish Embryo.

Environ Toxicol Chem 2021 Jul 20. Epub 2021 Jul 20.

Zebrafishlab, Veterinary, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610, Wilrijk, Belgium.

Non-polar narcosis, also known as baseline toxicity, has been described as the minimal toxicity that an organic chemical may elicit based on its lipophilicity. While lethal effects of narcosis-inducing chemicals (NICs) have been thoroughly investigated, knowledge of sublethal effects is still very limited. We investigated the effects of three well-known NICs (phenanthrene, 1,3,5-trichlorobenzene and pentachlorobenzene) on a variety of organismal endpoints (malformations, swim bladder inflation, respiration, heart rate, swimming activity and turning angles), which can be plausibly linked to narcosis in zebrafish embryos. Read More

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Short-term dietary restriction maintains synaptic plasticity whereas short-term overfeeding alters cellular dynamics in the aged brain: evidence from the zebrafish model organism.

Neurobiol Aging 2021 Jun 19;106:169-182. Epub 2021 Jun 19.

Interdisciplinary Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University, Ankara, Turkey; National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey; Department of Molecular Biology and Genetics, Zebrafish Facility, Bilkent University, Ankara, Turkey; Department of Psychology, Bilkent University, Ankara, Turkey. Electronic address:

Increased caloric intake (OF) impairs quality of life causing comorbidities with other diseases and cognitive deficits, whereas dietary restriction (DR) increases healthspan by preventing age-related deteriorations. To understand the effects of these opposing dietary regimens on the cellular and synaptic dynamics during brain aging, the zebrafish model, which shows gradual aging like mammals, was utilized. Global changes in cellular and synaptic markers with respect to age and a 12 week dietary regimen of OF and DR demonstrated that aging reduces the levels of the glutamate receptor subunits, GLUR2/3, inhibitory synaptic clustering protein, GEP, synaptic vesicle protein, SYP, and early-differentiated neuronal marker, HuC. Read More

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Effects of 4-epianhydrotetracycline on oxidative stress in zebrafish (Danio rerio) embryos.

Sci Total Environ 2021 Jul 14;796:149047. Epub 2021 Jul 14.

College of Biology and Environmental Science, Jishou University, Jishou 416000, PR China.

4-Epianhydrotetracycline (4-EATC) is a major intermediate product of tetracycline during its degradation progress in the natural environment, which is frequently detected in aquatic environments and poses a potential threat to aquatic organisms. In the present study, the ecotoxicity of 4-EATC have been studied from the perspective of oxidative stress by using zebrafish embryos. After 96 h exposure, the reactive oxygen species (ROS) levels, malondialdehyde (MDA) concentrations and protein carbonyl (PC) contents in zebrafish embryos in the lower-concentration 4-EATC treatment groups (1. Read More

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Cortisol and glucocorticoid receptor 2 regulate acid secretion in medaka (Oryzias latipes) larvae.

J Comp Physiol B 2021 Jul 17. Epub 2021 Jul 17.

Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 11529, Taiwan.

Freshwater fish live in environments where pH levels fluctuate more than those in seawater. During acidic stress, the acid-base balance in these fish is regulated by ionocytes in the gills, which directly contact water and function as an external kidney. In ionocytes, apical acid secretion is largely mediated by H-ATPase and the sodium/hydrogen exchanger (NHE). Read More

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Myelination induces axonal hotspots of synaptic vesicle fusion that promote sheath growth.

Curr Biol 2021 Jul 8. Epub 2021 Jul 8.

Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK. Electronic address:

Myelination of axons by oligodendrocytes enables fast saltatory conduction. Oligodendrocytes are responsive to neuronal activity, which has been shown to induce changes to myelin sheaths, potentially to optimize conduction and neural circuit function. However, the cellular bases of activity-regulated myelination in vivo are unclear, partly due to the difficulty of analyzing individual myelinated axons over time. Read More

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Bioaccumulation, Metabolism and the Toxic Effects of Chlorfenapyr in Zebrafish ().

J Agric Food Chem 2021 Jul 16;69(29):8110-8119. Epub 2021 Jul 16.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Sciences, China Agricultural University, Beijing 100193, China.

Chlorfenapyr is widely used as an insecticide/miticide. Tralopyril, the active metabolite of chlorfenapyr, is used as an antifouling biocide in antifouling systems, and negatively affects aquatic environments. However, it is unclear whether tralopyril is a metabolite of chlorfenapyr in aquatic vertebrates, and there is little data on the bioaccumulation and toxicity of chlorfenapyr to aquatic vertebrates. Read More

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The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects.

Front Cell Dev Biol 2021 29;9:702969. Epub 2021 Jun 29.

The Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Objective: Congenital eye diseases are multi-factorial and usually cannot be cured. Therefore, proper preventive strategy and understanding the pathomechanism underlying these diseases become important. Deficiency in folate, a water-soluble vitamin B, has been associated with microphthalmia, a congenital eye disease characterized by abnormally small and malformed eyes. Read More

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Loss of Deacetylation Enzymes Hdac6 and Sirt2 Promotes Acetylation of Cytoplasmic Tubulin, but Suppresses Axonemal Acetylation in Zebrafish Cilia.

Front Cell Dev Biol 2021 28;9:676214. Epub 2021 Jun 28.

The Bateson Centre, The University of Sheffield, Sheffield, United Kingdom.

Cilia are evolutionarily highly conserved organelles with important functions in many organs. The extracellular component of the cilium protruding from the plasma membrane comprises an axoneme composed of microtubule doublets, arranged in a 9 + 0 conformation in primary cilia or 9 + 2 in motile cilia. These microtubules facilitate transport of intraflagellar cargoes along the axoneme. Read More

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SP1-Mediated Upregulation of Long Noncoding RNA ZFAS1 Involved in Non-syndromic Cleft Lip and Palate Inactivating WNT/β-Catenin Signaling Pathway.

Front Cell Dev Biol 2021 29;9:662780. Epub 2021 Jun 29.

ENT Institute, Department of Facial Plastic and Reconstructive Surgery, Eye & ENT Hospital, Fudan University, Shanghai, China.

Non-syndromic cleft lip and palate (NSCLP) is one of the most common congenital malformations with multifactorial etiology. Although long non-coding RNAs (lncRNAs) have been implicated in the development of lip and palate, their roles in NSCLP are not fully elucidated. This study aimed to investigate how dysregulated lncRNAs contribute to NSCLP. Read More

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Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model.

Sci Rep 2021 Jul 12;11(1):14289. Epub 2021 Jul 12.

School of Pharmacy, Southwest University, Chongqing, China.

Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Read More

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