90 results match your criteria level actinobacterial


sp. nov., an endophytic actinomycete isolated from the root of (L.).

Int J Syst Evol Microbiol 2021 Jun;71(6)

Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

An endophytic actinobacterium, designated strain CA1R205, was isolated from the surface-sterilized root of L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. Read More

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Diversity and prevalence of ANTAR RNAs across actinobacteria.

BMC Microbiol 2021 May 29;21(1):159. Epub 2021 May 29.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bellary Road, Bangalore, 560065, India.

Background: Computational approaches are often used to predict regulatory RNAs in bacteria, but their success is limited to RNAs that are highly conserved across phyla, in sequence and structure. The ANTAR regulatory system consists of a family of RNAs (the ANTAR-target RNAs) that selectively recruit ANTAR proteins. This protein-RNA complex together regulates genes at the level of translation or transcriptional elongation. Read More

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Green Synthesized Silver Nanoparticles: Antibacterial and Anticancer Activities, Biocompatibility, and Analyses of Surface-Attached Proteins.

Front Microbiol 2021 22;12:632505. Epub 2021 Apr 22.

Department of Microbiology, Nicolaus Copernicus University, Toruń, Poland.

The increasing number of multi-drug-resistant bacteria and cancer cases, that are a real threat to humankind, forces research world to develop new weapons to deal with it. Biogenic silver nanoparticles (AgNPs) are considered as a solution to this problem. Biosynthesis of AgNPs is regarded as a green, eco-friendly, low-priced process that provides small and biocompatible nanostructures with antimicrobial and anticancer activities and potential application in medicine. Read More

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Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert.

Front Microbiol 2021 15;12:604999. Epub 2021 Mar 15.

Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. Read More

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Biodegradation and Characterization of Streptomyces sp. (JMCACA3) from Acid Corroded Iron Plate.

Curr Microbiol 2021 Apr 25;78(4):1245-1255. Epub 2021 Feb 25.

Department of Comprehensive Dentistry, University of Texas Health Science Center, San Antonio, TX, USA.

From acid corroded iron plates five different types of actinobacteria were isolated. Among the five, JMCACA3 strain was selected for the present study. In ISP media, JMCACA3 strain showed well-developed aerial and substrate mycelia were observed. Read More

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Genomes of the " Actinomarinales" Order: Highly Streamlined Marine Epipelagic Actinobacteria.

mSystems 2020 Dec 15;5(6). Epub 2020 Dec 15.

Evolutionary Genomics Group, División de Microbiología, Universidad Miguel Hernández, San Juan, Alicante, Spain

" Actinomarinales" was defined as a subclass of exclusively marine with small cells and genomes. We have collected all the available genomes in databases to assess the diversity included in this group and analyzed it by comparative genomics. We have found the equivalent of five genera and 18 genomospecies. Read More

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December 2020

Grazing pressure-induced shift in planktonic bacterial communities with the dominance of acIII-A1 actinobacterial lineage in soda pans.

Sci Rep 2020 11 16;10(1):19871. Epub 2020 Nov 16.

Department of Microbiology, ELTE Eötvös Loránd University, Pázmány Péter stny. 1/C, Budapest, 1117, Hungary.

Astatic soda pans of the Pannonian Steppe are unique environments with respect to their multiple extreme physical and chemical characteristics (high daily water temperature fluctuation, high turbidity, alkaline pH, salinity, polyhumic organic carbon concentration, hypertrophic state and special ionic composition). However, little is known about the seasonal dynamics of the bacterial communities inhabiting these lakes and the role of environmental factors that have the main impact on their structure. Therefore, two soda pans were sampled monthly between April 2013 and July 2014 to reveal changes in the planktonic community. Read More

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November 2020

Streamlined and Abundant Bacterioplankton Thrive in Functional Cohorts.

mSystems 2020 Sep 29;5(5). Epub 2020 Sep 29.

Department of Ecology and Genetics, Limnology, Uppsala University, Uppsala, Sweden.

While fastidious microbes can be abundant and ubiquitous in their natural communities, many fail to grow axenically in laboratories due to auxotrophies or other dependencies. To overcome auxotrophies, these microbes rely on their surrounding cohort. A cohort may consist of kin (ecotypes) or more distantly related organisms (community) with the cooperation being reciprocal or nonreciprocal and expensive (Black Queen hypothesis) or costless (by-product). Read More

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September 2020

Single-Cell Genomics of Novel Actinobacteria With the Wood-Ljungdahl Pathway Discovered in a Serpentinizing System.

Front Microbiol 2020 9;11:1031. Epub 2020 Jun 9.

Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo, Japan.

Serpentinite-hosted systems represent modern-day analogs of early Earth environments. In these systems, water-rock interactions generate highly alkaline and reducing fluids that can contain hydrogen, methane, and low-molecular-weight hydrocarbons-potent reductants capable of fueling microbial metabolism. In this study, we investigated the microbiota of Hakuba Happo hot springs (∼50°C; pH∼10. Read More

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Biogenic Silver Nanoparticles: Assessment of Their Cytotoxicity, Genotoxicity and Study of Capping Proteins.

Molecules 2020 Jul 2;25(13). Epub 2020 Jul 2.

Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87100 Torun, Poland.

The development of nanotechnology in the last two decades has led to the use of silver nanoparticles (AgNPs) in various biomedical applications, including antimicrobial, anti-inflammatory, and anticancer therapies. However, the potential of the medical application of AgNPs depends on the safety of their use. In this work, we assessed the in vitro cytotoxicity and genotoxicity of silver nanoparticles and identified biomolecules covering AgNPs synthesized from actinobacterial strain SH11. Read More

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gen. nov., sp. nov., a new member of the family isolated from a cave soil.

Int J Syst Evol Microbiol 2020 May;70(5):3340-3347

Jeju Biological Resource Co., Ltd, CTC business Incubator, Jeju 63242, Republic of Korea.

A novel Gram-stain-positive, actinobacterial strain, designated C5-26, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach. The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters. Colonies of the cells were circular, convex, entire and white. Read More

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Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA.

Cell Microbiol 2020 07 24;22(7):e13199. Epub 2020 Mar 24.

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.

Campylobacter jejuni is a bacterial pathogen that is generally acquired as a zoonotic infection from poultry and animals. Adhesion of C. jejuni to human colorectal epithelial cells is weakened after loss of its cj0588 gene. Read More

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Environmentally relevant concentrations of silver nanoparticles diminish soil microbial biomass but do not alter enzyme activities or microbial diversity.

J Hazard Mater 2020 06 4;391:122224. Epub 2020 Feb 4.

CEBAS-CSIC. Department of Soil and Water Conservation, Campus Universitario de Espinardo, 30100, Murcia, Spain.

The increasing use of silver nanoparticles (AgNPs) due to their well-known antimicrobial activity, has led to their accumulation in soil ecosystems. However, the impact of environmental realistic concentrations of AgNPs on the soil microbial community has been scarcely studied. In this work, we have assessed the impact of AgNPs, that mimic real concentrations in nature, on tropical soils cultivated with Coffea arabica under conventional and organic management systems. Read More

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Genome based characterization of Kitasatospora sp. MMS16-BH015, a multiple heavy metal resistant soil actinobacterium with high antimicrobial potential.

Gene 2020 Apr 20;733:144379. Epub 2020 Jan 20.

Department of Microbiology and Molecular Biology, 99 Daehak-Ro, Yuseong, Daejeon 34134, Chungnam National University, Daejeon, Republic of Korea. Electronic address:

An actinobacterial strain designated Kitasatospora sp. MMS16-BH015, exhibiting high level of heavy metal resistance, was isolated from soil of an abandoned metal mining site, and its potential for metal resistance and secondary metabolite production was studied. The strain was resistant to multiple heavy metals including zinc (up to 100 mM), nickel (up to 2 mM) and copper (up to 0. Read More

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Effect of "ribosome engineering" on the transcription level and production of S. albus indigenous secondary metabolites.

Appl Microbiol Biotechnol 2019 Sep 19;103(17):7097-7110. Epub 2019 Jul 19.

Department Pharmacy, Actinobacteria Metabolic Engineering Group, Saarland University, Uni Campus C2.3, 66123, Saarbrücken, Germany.

Significant resources are invested into efforts to improve the production yields of natural products from Actinobacteria, a well-recognized source of leads for several industries, most notably pharmaceutical one. Introduction of changes into genes for ribosomal protein S12 (rpsL) and/or 16S rRNA methylation (rsmG) is one of traditional approaches (referred to as ribosomal engineering) towards actinobacterial strain improvement. Yet, true potential of ribosome engineering remains unknown as it is currently coupled to empirical selection for aminoglycoside-resistance; rpsL mutations without such phenotypic expression could not be isolated. Read More

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September 2019

Identification of Surf1 as an assembly factor of the cytochrome bc-aa supercomplex of Actinobacteria.

Biochim Biophys Acta Bioenerg 2019 10 19;1860(10):148033. Epub 2019 Jun 19.

Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425 Jülich, Germany. Electronic address:

Respiration in aerobic Actinobacteria involves a cytochrome bc-aa supercomplex with a diheme cytochrome c, first isolated from Corynebacterium glutamicum. Synthesis of a functional cytochrome c oxidase requires incorporation of Cu, Cu, heme a, and heme a. In contrast to eukaryotes and α-proteobacteria, this process is poorly understood in Actinobacteria. Read More

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October 2019

Gene miaA for post-transcriptional modification of tRNA is important for morphological and metabolic differentiation in Streptomyces.

Mol Microbiol 2019 07 6;112(1):249-265. Epub 2019 May 6.

Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st., Lviv, 79005, Ukraine.

Members of actinobacterial genus Streptomyces possess a sophisticated life cycle and are the deepest source of bioactive secondary metabolites. Although morphogenesis and secondary metabolism are subject to transcriptional co-regulation, streptomycetes employ an additional mechanism to initiate the aforementioned processes. This mechanism is based on delayed translation of rare leucyl codon UUA by the only cognate tRNA (encoded by bldA). Read More

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Ms1 RNA increases the amount of RNA polymerase in Mycobacterium smegmatis.

Mol Microbiol 2019 02 11;111(2):354-372. Epub 2018 Dec 11.

Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic.

Ms1 is a sRNA recently found in mycobacteria and several other actinobacterial species. Ms1 interacts with the RNA polymerase (RNAP) core devoid of sigma factors, which differs from 6S RNA that binds to RNAP holoenzymes containing the primary sigma factor. Here we show that Ms1 is the most abundant non-rRNA transcript in stationary phase in Mycobacterium smegmatis. Read More

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February 2019

Diversity of Bacteria and the Characteristics of Actinobacteria Community Structure in Badain Jaran Desert and Tengger Desert of China.

Front Microbiol 2018 23;9:1068. Epub 2018 May 23.

Peking Union Medical College, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing, China.

To assess the diversity of actinobacterial taxa in desert sands and obtain the novel microbial resources, 79 and 50 samples were collected from the Badain Jaran (BJD) and Tengger Deserts (TGD) of China, respectively. High-throughput sequencing (HTS) of environmental 16S rRNA genes within these samples was conducted on an Illumina Miseq platform, using universal bacterial primers targeting the V3-V4 hypervariable region. Based on the HTS analyses, cultivation-dependent (CULD) techniques were optimized to identify the cultivable members. Read More

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Isolation and Identification of an Anthracimycin Analogue from , a Halophile from a Saltern, by Genomic Mining Strategy.

J Genomics 2018 21;6:63-73. Epub 2018 May 21.

Bioinformatics Institute, Agency for Science, Technology and Research (ASTAR), 30 Biopolis Street, #07-01 Matrix, Singapore 138671, Republic of Singapore.

Modern medicine is unthinkable without antibiotics; yet, growing issues with microbial drug resistance require intensified search for new active compounds. Natural products generated by Actinobacteria have been a rich source of candidate antibiotics, for example anthracimycin that, so far, is only known to be produced by species. Based on sequence similarity with the respective biosynthetic cluster, we sifted through available microbial genome data with the goal to find alternative anthracimycin-producing organisms. Read More

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Extracellular Synthesis and Characterization of Gold Nanoparticles Using sp. BRS2A-AR2 Isolated from the Aerial Roots of the Ghanaian Mangrove Plant, .

Indian J Microbiol 2018 Jun 22;58(2):214-221. Epub 2018 Jan 22.

2Department of Chemistry, School of Physical and Mathematical Sciences, University of Ghana, P.O. Box LG 56, Legon-Accra, Ghana.

Through the use of genomes that have undergone millions of years of evolution, marine are known to have adapted to rapidly changing environmental pressures. The result is a huge chemical and biological diversity among marine . It is gradually becoming a known fact that, marine have the capability to produce nanoparticles which have reasonable sizes and structures with possible applications in biotechnology and pharmacology. Read More

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High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits.

Antibiotics (Basel) 2018 Mar 21;7(2). Epub 2018 Mar 21.

InBioS-Centre for Protein Engineering, Institut de Chimie B6a, University of Liège, B-4000 Liège, Belgium.

Moonmilk are cave carbonate deposits that host a rich microbiome, including antibiotic-producing Actinobacteria, making these speleothems appealing for bioprospecting. Here, we investigated the taxonomic profile of the actinobacterial community of three moonmilk deposits of the cave "Grotte des Collemboles" via high-throughput sequencing of 16S rRNA amplicons. Actinobacteria was the most common phylum after Proteobacteria, ranging from 9% to 23% of the total bacterial population. Read More

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Genomic Insights into Evolution of AdpA Family Master Regulators of Morphological Differentiation and Secondary Metabolism in Streptomyces.

J Mol Evol 2018 04 13;86(3-4):204-215. Epub 2018 Mar 13.

Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho St. 4, Lviv, 79005, Ukraine.

The AdpA protein from a streptomycin producer Streptomyces griseus is a founding member of the AdpA family of pleiotropic regulators, known to be ubiquitously present in streptomycetes. Functional genomic approaches revealed a huge number of AdpA targets, leading to the claim that the AdpA regulon is the largest one in bacteria. The expression of adpA is limited at the level of translation of the rare leucyl UUA codon. Read More

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Microbial cycling of isoprene, the most abundantly produced biological volatile organic compound on Earth.

ISME J 2018 04 20;12(4):931-941. Epub 2018 Feb 20.

School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.

Isoprene (2-methyl-1,3-butadiene), the most abundantly produced biogenic volatile organic compound (BVOC) on Earth, is highly reactive and can have diverse and often detrimental atmospheric effects, which impact on climate and health. Most isoprene is produced by terrestrial plants, but (micro)algal production is important in aquatic environments, and the relative bacterial contribution remains unknown. Soils are a sink for isoprene, and bacteria that can use isoprene as a carbon and energy source have been cultivated and also identified using cultivation-independent methods from soils, leaves and coastal/marine environments. Read More

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Contrasting patterns of genome-level diversity across distinct co-occurring bacterial populations.

ISME J 2018 03 8;12(3):742-755. Epub 2017 Dec 8.

Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.

To understand the forces driving differentiation and diversification in wild bacterial populations, we must be able to delineate and track ecologically relevant units through space and time. Mapping metagenomic sequences to reference genomes derived from the same environment can reveal genetic heterogeneity within populations, and in some cases, be used to identify boundaries between genetically similar, but ecologically distinct, populations. Here we examine population-level heterogeneity within abundant and ubiquitous freshwater bacterial groups such as the acI Actinobacteria and LD12 Alphaproteobacteria (the freshwater sister clade to the marine SAR11) using 33 single-cell genomes and a 5-year metagenomic time series. Read More

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Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG29 isolated from a Saharan soil.

J Mycol Med 2018 Mar 20;28(1):150-160. Epub 2017 Nov 20.

Laboratoire de biologie des systèmes microbiens (LBSM), école normale supérieure de Kouba, Alger, Algeria.

An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99. Read More

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High altitude, hyper-arid soils of the Central-Andes harbor mega-diverse communities of actinobacteria.

Extremophiles 2018 Jan 3;22(1):47-57. Epub 2017 Nov 3.

School of Biology, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.

The data reported in this paper are among the first relating to the microbiology of hyper-arid, very high altitude deserts and they provide base line information on the structure of actinobacterial communities. The high mountain Cerro Chajnantor landscape of the Central Andes in northern Chile is exposed to the world's most intense levels of solar radiation and its impoverished soils are severely desiccated. The purpose of this research was to define the actinobacterial community structures in soils at altitudes ranging from 3000 to 5000 m above sea level. Read More

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January 2018

Biocompatible silver, gold and silver/gold alloy nanoparticles for enhanced cancer therapy: in vitro and in vivo perspectives.

Nanoscale 2017 Nov;9(43):16773-16790

Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.

The aims of nano oncology are to detect, target and treat cancer cells without any side effects. The present study describes the microbial synthesis of biocompatible nanoparticles of silver (AgNPs), gold (AuNPs) and their alloy (Ag/AuNPs) for hepatoprotective activity against diethylnitrosamine (DEN)-induced liver cancer in a Sprague Dawley (SD) rat model. The crystalline nature and physicochemical features of the nanoparticles were identified by Fourier transform infra-red (FT-IR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and selected area electron diffraction (SAED) analysis. Read More

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November 2017

De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii.

BMC Genomics 2017 Oct 16;18(1):790. Epub 2017 Oct 16.

Department of Food and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.

Background: Propionibacterium freudenreichii is an industrially important bacterium granted the Generally Recognized as Safe (the GRAS) status, due to its long safe use in food bioprocesses. Despite the recognized role in the food industry and in the production of vitamin B12, as well as its documented health-promoting potential, P. freudenreichii remained poorly characterised at the genomic level. Read More

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October 2017

Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline.

Appl Environ Microbiol 2017 Dec 1;83(24). Epub 2017 Dec 1.

Instituto de Investigación de la Viña y el Vino, Universidad de León, León, Spain

Endophytic and rhizosphere actinobacteria isolated from the root system of 1-year-old grafted plants were evaluated for their activities against fungi that cause grapevine trunk diseases. A total of 58 endophytic and 94 rhizosphere isolates were tested. Based on an bioassay, 15. Read More

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December 2017