464 results match your criteria leptospira isolates


Application of simplified MLST scheme for direct typing of clinical samples from human leptospirosis cases in a tertiary hospital in the Philippines.

PLoS One 2021 20;16(10):e0258891. Epub 2021 Oct 20.

Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.

Despite the major threat of leptospirosis to public health in the Philippines, its epidemiologic data remain scarce. Multilocus sequence typing (MLST) is a method often used for identification of circulating Leptospira species and disease surveillance. Unfortunately, molecular typing of Leptospira isolates is not routinely done in most hospital settings. Read More

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October 2021

Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients.

Genomics Inform 2021 Sep 30;19(3):e31. Epub 2021 Sep 30.

Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. Read More

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September 2021

Complete Genome Sequencing of Isolates from Malaysia Reveals Massive Genome Rearrangement but High Conservation of Virulence-Associated Genes.

Pathogens 2021 Sep 15;10(9). Epub 2021 Sep 15.

Research Group Biomarkers for Infectious Diseases, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

The ability of Leptospirae to persist in environments and animal hosts but to cause clinically highly variable disease in humans has made leptospirosis the most common zoonotic disease. Considering the paucity of data on variation in complete genomes of human pathogenic Leptospirae, we have used a combination of Single Molecule Real-Time (SMRT) and Illumina sequencing to obtain complete genome sequences of six human clinical isolates from Malaysia. All six contained the larger (4. Read More

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September 2021

Shedding and Genetic Diversity of Leptospira spp. From Urban Stray Dogs in Klang Valley, Malaysia.

Top Companion Anim Med 2021 Jul 13;45:100562. Epub 2021 Jul 13.

Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Leptospirosis is an endemic zoonoses of global proportions. Stray dogs have been postulated to play a role in disease transmission; however, supporting information are still limited. Roaming behavior may not only predispose the dogs to infection, but could also contribute to disease spread. Read More

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New Insights on Infections in a Canine Population from North Sardinia, Italy: A Sero-Epidemiological Study.

Biology (Basel) 2021 Jun 7;10(6). Epub 2021 Jun 7.

Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy.

Leptospirosis is a widespread zoonosis recognized as a re-emerging infectious disease in a wide variety of animal species, including humans and dogs. No data exist regarding the presence of species in the canine population of Sardinia Island. This study reports the first sero-survey for leptospirosis in kennel and owned dogs from six areas of the north of Sardinia. Read More

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Molecular and serological characterization of pathogenic Leptospira spp. isolated from symptomatic dogs in a highly endemic area, Brazil.

BMC Vet Res 2021 Jun 21;17(1):221. Epub 2021 Jun 21.

Universidade Santo Amaro, São Paulo, SP, Brazil.

Background: Leptospirosis is an endemic zoonosis in Brazil, with a great impact on human and animal health. Although dogs are frequently infected by pathogenic Leptospira, the current epidemiological understanding of canine leptospirosis is mainly based on serological tests that predict the infecting serogroup/serovar. Thus, the present study aimed at identifying the causative agent for severe cases of canine leptospirosis in a highly endemic area through the isolation and characterization of the isolated strains. Read More

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Detection and characterization of spp. in dogs diagnosed with kidney and/or liver disease in Selangor, Malaysia.

J Vet Diagn Invest 2021 Sep 19;33(5):834-843. Epub 2021 Jun 19.

Departments of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Read More

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September 2021

Multidisciplinary approach in the diagnosis of acute leptospirosis in dogs naturally infected by Leptospira interrogans serogroup Icterohaemorrhagiae: A prospective study.

Comp Immunol Microbiol Infect Dis 2021 Aug 7;77:101664. Epub 2021 May 7.

Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil. Electronic address:

Leptospirosis, a zoonotic disease with worldwide distribution, is caused by spirochetes of the genus Leptospira. In dogs, this disease is frequently misdiagnosed. Few studies have attempted to associate the detection of Leptospira spp. Read More

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Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan.

Sci Rep 2021 05 14;11(1):10365. Epub 2021 May 14.

Department of Bacteriology I, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.

Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Cats have been reported to be infected with Leptospira spp. and shed the bacteria in the urine. Read More

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Molecular Typing of Pathogenic Species Isolated from Wild Mammal Reservoirs in Sardinia.

Animals (Basel) 2021 Apr 13;11(4). Epub 2021 Apr 13.

Laboratory of Sieroimmunology, Animal Health Department, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy.

Leptospirosis is a global zoonosis caused by pathogenic species of that infect a large spectrum of domestic and wild animals. This study is the first molecular identification, characterization, and phylogeny of strains with veterinary and zoonotic impact in Sardinian wild hosts. All samples collected were cultured and analyzed by multiplex real time polymerase chain reaction (qPCR). Read More

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Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs () in Tuscany (Italy).

Animals (Basel) 2021 Apr 16;11(4). Epub 2021 Apr 16.

Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.

Domestic dogs () used for wild boar () hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti- antibodies and the occurrence, antimicrobial resistance, and virulence of spp., , and in sera and rectal swabs collected from 42 domestic hunting dogs in the Tuscany region (Italy). Read More

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Molecular epidemiology of Leptospira spp. among wild mammals and a dog in Amami Oshima Island, Japan.

PLoS One 2021 22;16(4):e0249987. Epub 2021 Apr 22.

Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.

Leptospirosis is a worldwide zoonosis caused by the pathogenic Leptospira spp. Canine and human leptospirosis sometimes occur on Amami Oshima Island, located in the Nansei Archipelago, southwestern Japan; however, information on the causative Leptospira spp. on this island is quite limited. Read More

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September 2021

Leptospira spp in Rodents from Peridomestic Sites in Endemic Regions of Nicaragua.

Ecohealth 2020 12 8;17(4):469-476. Epub 2021 Apr 8.

Centro Veterinario de Diagnóstico e Investigación (CEVEDI), Escuela de Ciencias Agrarias y Veterinarias, Universidad Nacional Autónoma de Nicaragua-León, UNAN-León), Edificio Central, Contiguo a la Iglesia La Merced, Apartado Postal 68, León, Nicaragua.

Leptospirosis is one of the most important zoonoses in tropical countries, including Nicaragua, where it is considered endemic. The aim of this study was to determine the frequency of Leptospira spp in rodents captured from peridomestic sites in leptospirosis endemic regions of Nicaragua. Using live traps, 191 rodents were captured in 2012 and 2013 between April and December. Read More

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December 2020

Detected in Testicles and Epididymis of Wild Boar ().

Biology (Basel) 2021 Mar 4;10(3). Epub 2021 Mar 4.

Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.

Leptospirosis is a re-emerging and worldwide diffused zoonosis. Recently, the high importance of their epidemiology was explained by the intermediate strains. Among these strains, was the first intermediate strain detected in domestic and wild swine. Read More

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Rat trade and leptospirosis: Molecular epidemiology of Leptospira species in rats exported from Cambodia to Vietnam.

Transbound Emerg Dis 2021 Mar 18. Epub 2021 Mar 18.

Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Rats are an important maintenance host of Leptospira spp., the causative agents of leptospirosis, a zoonotic disease with worldwide distribution. Rats are traded as food in the Mekong Delta, where Cambodia exports tons of rats to Vietnam. Read More

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12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka.

PLoS Negl Trop Dis 2021 03 18;15(3):e0009272. Epub 2021 Mar 18.

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America.

Leptospirosis is a ubiquitous zoonotic disease and a major clinical challenge owing to the multitude of clinical presentations and manifestations that are possibly attributable to the diversity of Leptospira, the understanding of which is key to study the epidemiology of this emerging global disease threat. Sri Lanka is a hotspot for leptospirosis with high levels of endemicity as well as annual epidemics. We carried out a prospective study of Leptospira diversity in Sri Lanka, covering the full range of climatic zones, geography, and clinical severity. Read More

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Leptospira interrogans serogroup Pomona strains isolated from river buffaloes.

Trop Anim Health Prod 2021 Mar 5;53(2):194. Epub 2021 Mar 5.

Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.

At present, little is known regarding the prevalence of buffalo leptospirosis worldwide, especially with respect to which Leptospira strains may infect this animal species. Furthermore, most investigations into this disease in buffaloes have only been performed with serological studies. In Brazil, particularly in the Amazon, buffalo production is growing and is just as important as cattle production, although few studies have been performed on buffalo compared to cattle. Read More

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The Effect of Environmental Stresses on Gene Expression in Pathogenic spp. through Real-Time PCR.

Pol J Microbiol 2020 Sep 8;69(3):301-310. Epub 2020 Sep 8.

Department of Genetic, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.

Leptospirosis is a worldwide infectious and zoonotic disease. The incidence of this disease is high in temperate regions, especially in northern Iran. The aim of this study was to investigate the effects of temperature, pH, and plant extract on the gene expression in pathogenic spp. Read More

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September 2020

Identification of Leptospira spp. in the animal-environment interface (swine-water) in pig production cycle.

Trop Anim Health Prod 2021 Feb 8;53(1):155. Epub 2021 Feb 8.

Department of Basics Sciences. Molecular Biology and Immunogenetics Research Group (BIOMIGEN), Universidad de La Salle, Carrera 2 No. 10 - 70, Bogotá, Colombia.

Introduction: Leptospirosis, caused by Leptospira spp., is a zoonosis with multiple hosts and reservoirs. Thus, the "One Health" approach must be addressed in order to guide prevention and control measures regarding this microorganism. Read More

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February 2021

Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy.

PLoS Negl Trop Dis 2020 12 28;14(12):e0008982. Epub 2020 Dec 28.

Department of Veterinary Science, University of Pisa, Pisa, Italy.

Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. Read More

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December 2020

Potentially Pathogenic in the Environment of an Elephant Camp in Thailand.

Trop Med Infect Dis 2020 Dec 6;5(4). Epub 2020 Dec 6.

The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.

is the causative agent of leptospirosis, a globally emerging zoonotic disease. The infection is commonly acquired through contact with the contaminated environment. To extend the knowledge on environmental source of leptospirosis, we investigated the presence of in an elephant camp setting where the interaction between humans, animals, and the shared environment occur particularly when engaging in recreational activities. Read More

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December 2020

Leptospira strains isolated from cattle in the Amazon region, Brazil, evidence of a variety of species and serogroups with a high frequency of the Sejroe serogroup.

Comp Immunol Microbiol Infect Dis 2021 Feb 15;74:101579. Epub 2020 Nov 15.

Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address:

In Brazil, there have been few leptospires isolated from cattle, especially in the Amazon, implying that the epidemiology of the disease in this region is still largely unclear. In a previous study, 52 Leptospira isolates were obtained from urine of cattle raised in the Brazilian Amazon and, to achieve a greater understanding of Leptospira infection in cattle of this region, the present study aimed to serologically and molecularly characterizes all these isolates. The laboratory assays used were the microscopic agglutination test (MAT) adopting a panel of polyclonal antisera against Leptospira spp. Read More

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February 2021

Characterization of a Novel Peptide from Pathogenic and Its Cytotoxic Effect.

Pathogens 2020 Oct 30;9(11). Epub 2020 Oct 30.

Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.

Leptospirosis is a zoonotic infectious disease caused by pathogenic species. Virulence proteins have been shown to be key determinants of the pathogenesis of pathogenic . A specific peptide at a mass-to-charge ratio of 7000 Da was identified in whole cells using matrix-assisted laser/desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Read More

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October 2020

Cats shedding pathogenic Leptospira spp.-An underestimated zoonotic risk?

PLoS One 2020 22;15(10):e0239991. Epub 2020 Oct 22.

Medizinische Kleintierklinik, Ludwig-Maximilians-Universität Munich, Munich, Germany.

Shedding of DNA of pathogenic Leptospira spp. has been documented in naturally infected cats in several countries, but urinary shedding of infectious Leptospira spp. has only recently been proven. Read More

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December 2020

Whole genome sequencing and de novo assembly of three virulent Indian isolates of Leptospira.

Infect Genet Evol 2020 11 2;85:104579. Epub 2020 Oct 2.

Gujarat State Biotechnology Mission, Department of Science & Technology, Government of Gujarat, Gandhinagar 382011, India; Gujarat Biotechnology Research Centre, Department of Science & Technology, Government of Gujarat, Gandhinagar 382011, India; Centre for Genomics and Biomedical Informatics, IMS and SUM Hospital, Siksha "O" Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003, India. Electronic address:

Leptospirosis is a re-emerging bacterial zoonosis caused by pathogenic Leptospira, with a worldwide distribution and becoming a major public health concern. Prophylaxis of this disease is difficult due to several factors such as non-specific variable clinical manifestation, presence of a large number of serovar, species and asymptomatic reservoir hosts, lack of proper diagnostics and vaccines. Despite its global importance and severity of the disease, knowledge about the molecular mechanism of pathogenesis and evolution of pathogenic species of Leptospira remains limited. Read More

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November 2020

Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua.

Trop Med Infect Dis 2020 Sep 18;5(3). Epub 2020 Sep 18.

Centro Veterinario de Diagnóstico e Investigación (CEVEDI), Departamento de Veterinaria y Zootecnia, Escuela de Ciencias Agrarias y Veterinarias, Universidad Nacional Autónoma de Nicaragua-León (UNAN-León), Carretera a La Ceiba 1 Km al Este, León 21000, Nicaragua.

In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases. Read More

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September 2020

Multilocus sequence typing reveals diverse known and novel genotypes of Leptospira spp. circulating in Sri Lanka.

PLoS Negl Trop Dis 2020 08 25;14(8):e0008573. Epub 2020 Aug 25.

Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Background: Leptospirosis has gained much attention in Sri Lanka since its large outbreak in 2008. However, most of the cases were clinically diagnosed and information on Leptospira genotypes and serotypes currently prevailing in the country is lacking.

Methodology/principal Findings: We retrospectively analyzed 24 Leptospira strains from human patients as well as isolated and characterized three Leptospira strains from black rats using the microscopic agglutination test with antisera for 19 serovars and multilocus sequence typing. Read More

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Case Report: Recovery of Pathogenic spp. from Routine Aerobic Blood Culture Bottles.

Am J Trop Med Hyg 2020 11;103(5):1834-1837

Department of Medicine, Mahidol University, Bangkok, Thailand.

spp. are fastidious and slow-growing bacteria, making recovery difficult and diagnostic sensitivity in the clinical setting low. However, collection of isolates is valuable for epidemiological and laboratory research. Read More

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November 2020

Genetic relatedness of Leptospira interrogans serogroup Autumnalis isolated from humans, dogs, and mice in Japan.

BMC Res Notes 2020 Aug 3;13(1):369. Epub 2020 Aug 3.

Department of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.

Objective: Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of Leptospira spp., and peridomiciliary rodents are the most important reservoir animals for human infection. Dogs are known to be the reservoir animal of L. Read More

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[Isolation and identification of in patients with fever of unknown origin in Guizhou province].

Zhonghua Liu Xing Bing Xue Za Zhi 2020 Jul;41(7):1110-1114

Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.

To understand the etiological characteristics of the patients with fever of unknown origin in Guizhou province through the isolation and identification of and provide evidence for the control, prevention and treatment of human leptospirosis. Blood and urine samples were collected from patients with fever symptoms in Qiandongnan, an epidemic area, in Guizhou. The suspected strains were primarily identified using pathogenic specific G1/G2-PCR, and subsequently identified by using serogroups specific PCR. Read More

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