3,231 results match your criteria leptospira interrogans


Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis.

Acta Trop 2021 Oct 20:106214. Epub 2021 Oct 20.

Instituto de Patobiología- UEDD IPVET INTA CONICET, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Argentina.

Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA. Read More

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October 2021

Application of simplified MLST scheme for direct typing of clinical samples from human leptospirosis cases in a tertiary hospital in the Philippines.

PLoS One 2021 20;16(10):e0258891. Epub 2021 Oct 20.

Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.

Despite the major threat of leptospirosis to public health in the Philippines, its epidemiologic data remain scarce. Multilocus sequence typing (MLST) is a method often used for identification of circulating Leptospira species and disease surveillance. Unfortunately, molecular typing of Leptospira isolates is not routinely done in most hospital settings. Read More

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October 2021

Role of the major determinant of polar flagellation FlhG in the endoflagella-containing spirochete Leptospira.

Mol Microbiol 2021 Oct 17. Epub 2021 Oct 17.

Institut Pasteur, Biology of Spirochetes Unit, Paris, France.

Spirochetes can be distinguished from other bacteria by their spiral-shaped morphology and subpolar periplasmic flagella. This study focused on FlhF and FlhG, which control the spatial and numerical regulation of flagella in many exoflagellated bacteria, in the spirochete Leptospira. In contrast to flhF which seems to be essential in Leptospira, we demonstrated that flhG mutants in both the saprophyte L. Read More

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October 2021

Comparative genome characterization of Leptospira interrogans from mild and severe leptospirosis patients.

Genomics Inform 2021 Sep 30;19(3):e31. Epub 2021 Sep 30.

Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Leptospirosis is a zoonotic disease caused by spirochetes from the genus Leptospira. In Thailand, Leptospira interrogans is a major cause of leptospirosis. Leptospirosis patients present with a wide range of clinical manifestations from asymptomatic, mild infections to severe illness involving organ failure. Read More

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September 2021

Expression and preliminary characterization of the potential vaccine candidate LipL32 of leptospirosis.

Appl Nanosci 2021 Sep 30:1-15. Epub 2021 Sep 30.

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu India.

Leptospirosis is a globally re-emerging infectious disease mainly for mammals. The infection is caused by the spirochete Gram-negative bacterium , which affects animals and humans worldwide. In our previous studies, recombinant protein production has been obtained from the bacterial expression system. Read More

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September 2021

Seroprevalence and Associated Risk Factors of Serogroup Serovar in Dairy Farms in and around Jimma Town, Southwestern Ethiopia.

Vet Med Int 2021 17;2021:6061685. Epub 2021 Sep 17.

Jimma University, School of Veterinary Medicine, Jimma, Ethiopia.

A cross-sectional study was conducted on selected dairy farms in and around Jimma town, Oromia, southwestern Ethiopia from November 2019 to May 2020 to determine the seroprevalence of (). Furthermore, information was gathered on individual animal and herd level by using pretested semistructured questionnaire to assess associated risk factors. A stratified and simple random sampling procedure was used for the selection of dairy farms and individual animal's, respectively. Read More

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September 2021

Gut microbiota involved in leptospiral infections.

ISME J 2021 Sep 29. Epub 2021 Sep 29.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, 130062, PR China.

Leptospirosis is a re-emerging zoonotic disease worldwide. Intestinal bleeding is a common but neglected symptom in severe leptospirosis. The regulatory mechanism of the gut microbiota on leptospirosis is still unclear. Read More

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September 2021

Complete Genome Sequencing of Isolates from Malaysia Reveals Massive Genome Rearrangement but High Conservation of Virulence-Associated Genes.

Pathogens 2021 Sep 15;10(9). Epub 2021 Sep 15.

Research Group Biomarkers for Infectious Diseases, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

The ability of Leptospirae to persist in environments and animal hosts but to cause clinically highly variable disease in humans has made leptospirosis the most common zoonotic disease. Considering the paucity of data on variation in complete genomes of human pathogenic Leptospirae, we have used a combination of Single Molecule Real-Time (SMRT) and Illumina sequencing to obtain complete genome sequences of six human clinical isolates from Malaysia. All six contained the larger (4. Read More

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September 2021

High Diversity of Species Infecting Bats Captured in the Urabá Region (Antioquia-Colombia).

Microorganisms 2021 Sep 7;9(9). Epub 2021 Sep 7.

Basic Science Research Group, Graduate School-CES University, Medellín 50021, Colombia.

Leptospirosis is a globally distributed zoonotic disease caused by pathogenic bacteria of the genus . This zoonotic disease affects humans, domestic animals and wild animals. Colombia is considered an endemic country for leptospirosis; Antioquia is the second department in Colombia, with the highest number of reported leptospirosis cases. Read More

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September 2021

Exposure of Free-Ranging Wild Animals to Zoonotic Sensu Stricto in Slovenia.

Animals (Basel) 2021 Sep 17;11(9). Epub 2021 Sep 17.

Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia.

A total of 249 serum samples from 13 wild animal species namely fallow deer (, = 1), roe deer (, = 80), red deer (, = 22), chamois (, = 21), mouflon ( = 4), brown hare (, = 2), nutria (, = 1), red fox (, = 97), stone marten (, = 12), European badger (, = 2), golden jackal (, = 2) Eurasian lynx (, = 2) and grey wolf (, = 3) were analysed for the presence of antibodies against sensu stricto. Serum samples were examined via the microscopic agglutination test for the presence of specific antibodies against serovars Icterohaemorrhagiae, Bratislava, Pomona, Grippotyphosa, Hardjo, Sejroe, Australis, Autumnalis, Canicola, Saxkoebing and Tarassovi. Antibodies to at least one of the pathogenic serovars were detected in 77 (30. Read More

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September 2021

Influence of Season, Population and Individual Characteristics on the Prevalence of spp. in Bank Voles in North-West Germany.

Biology (Basel) 2021 Sep 18;10(9). Epub 2021 Sep 18.

Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, Germany.

Leptospirosis is a worldwide zoonotic disease with more than 1 million human cases annually. Infections are associated with direct contact to infected animals or indirect contact to contaminated water or soil. As not much is known about the prevalence and host specificity of spp. Read More

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September 2021

Antigen-Specific Urinary Immunoglobulin in Reservoir Hosts of Leptospirosis.

Vet Sci 2021 Sep 1;8(9). Epub 2021 Sep 1.

Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA 50010, USA.

Domestic and wildlife animal species act as reservoir hosts of leptospirosis, a global zoonotic disease affecting more than 1 million people annually and causing significant morbidity and mortality in domestic animals. In contrast to incidental hosts which present with an array of clinical manifestations, reservoir hosts are typically asymptomatic and can shed leptospires from chronically infected kidneys via urine for extended periods of time. Renal excretion of leptospires occurs despite evidence of a humoral and cellular immune response and is reflective of the unique biological equilibrium that exists between certain animal species and specific serovars of . Read More

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September 2021

Are infectious reproductive pathogens of large ruminants a threat to improving food security? An investigation from Cambodia.

Trop Anim Health Prod 2021 Sep 24;53(5):480. Epub 2021 Sep 24.

The University of Sydney, Sydney School of Veterinary Science, 425 Werombi Road, Camden, NSW, 2570, Australia.

In Cambodia, information on common pathogens causing reproductive losses in cattle and buffalo are lacking, despite there being a need to address livestock health to enhance food security. We analysed stored buffalo (n = 29) and cattle (n = 471) serum samples collected in 2016 using commercially available enzyme-linked immunosorbent assay kits. Antibodies to Neospora caninum, bovine viral diarrhoea virus (BVDV), Leptospira interrogans serovar Hardjo and Brucella abortus were detected in buffalo samples at 79. Read More

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September 2021

Draft Genome Sequences of Four Different Strains Belonging to Leptospira interrogans Serovar Pomona Isolated from Mammals in the Island of Sardinia, Italy.

Microbiol Resour Announc 2021 Sep 23;10(38):e0069821. Epub 2021 Sep 23.

Istituto Zooprofilattico Sperimentale of Sardinia, Animal Health Department, Sassari, Italy.

Leptospirosis is a zoonotic disease of global importance caused by a pathogenic group of bacteria belonging to the genus . Here, we report four draft genome sequences of Leptospira interrogans serovar Pomona isolated in Sardinia (Italy) from four different species of mammals (i.e. Read More

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September 2021

Identification of in vivo expressed proteins in live attenuated lipopolysaccharide mutant that mediates heterologous protection against Leptospira spp.

Vet Microbiol 2021 Aug 30;262:109220. Epub 2021 Aug 30.

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Chula Vaccine Research Center (Chula VRC), Center of Excellence in Vaccine Research and Development, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:

Leptospirosis vaccines that elicit broad protection against a range of pathogenic Leptospira spp. would overcome a major drawback of currently licensed bacterin vaccines. Live attenuated vaccine produced from a lipopolysaccharide (LPS) mutant strain of L. Read More

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Leptospira interrogans biofilm formation in Rattus norvegicus (Norway rats) natural reservoirs.

PLoS Negl Trop Dis 2021 Sep 8;15(9):e0009736. Epub 2021 Sep 8.

Institute of Biology, Federal University of Bahia, Salvador, Brazil.

Rattus norvegicus (Norway rat) is the main reservoir host of pathogenic Leptospira, the causative agent of leptospirosis, in urban environments. Pathogenic Leptospira forms biofilms in the environment, possibly contributing for bacterial survival and maintenance. Nonetheless, biofilms have not yet been studied in natural animal reservoirs presenting leptospiral renal carriage. Read More

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September 2021

Bovine Genital Leptospirosis and reproductive disorders of live subfertile cows under field conditions.

Vet Microbiol 2021 Oct 23;261:109213. Epub 2021 Aug 23.

Laboratory of Veterinary Bacteriology, Biomedical Institute, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address:

Bovine genital leptospirosis (BGL) is characterized by silent chronic reproductive disorders, most related to early embryonic death leading to estrus repetition, subfertility and abortions. However, most studies were conducted in slaughterhouses, which lacks reproductive and sanitary history of the studied animals. This study aimed to evaluate the occurrence of Leptospira sp. Read More

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October 2021

Historical Spatial Distribution of Zoonotic Diseases in Domestic, Synanthropic, and Wild Animals in the Mexican Territory of the Yucatan Peninsula.

J Trop Med 2021 7;2021:8699455. Epub 2021 Aug 7.

Laboratorio de Parasitología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Mérida, Mexico.

The Mexican territory of the Yucatan Peninsula has a tropical climate and harbors a wide variety of domestic, synanthropic, and wild animals, as well as disease vectors. To determine the distribution of recorded zoonotic diseases in the Yucatan Peninsula, scientific publications referring to these diseases in animals and containing geographic coordinates of disease occurrence, were studied. The epidemiological bulletins of the national government were also consulted to obtain information on zoonotic diseases reported in humans in the territory. Read More

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Protective efficacy of whole-cell inactivated Leptospira vaccines made using virulent or avirulent strains in a hamster model.

Vaccine 2021 09 13;39(39):5626-5634. Epub 2021 Aug 13.

Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

Whole-cell inactivated vaccines remain the only licensed vaccines used to control human and animal leptospirosis worldwide. Although they are protective against lethal infections, the efficacy of these vaccines has been divergent. The manufacturing process often involves the use of standard bacterial strains subjected to serial in vitro passages, with a risk of loss of virulence, and may affect the immunogenicity and consequently decrease protection. Read More

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September 2021

Pathology and One Health implications of fatal Leptospira interrogans infection in an urbanized, free-ranging, black-tufted marmoset (Callithrix penicillata) in Brazil.

Transbound Emerg Dis 2021 Aug 13. Epub 2021 Aug 13.

Veterinary Pathology Laboratory, University of Brasília, Brasília, Brazil.

Leptospirosis is a zoonotic neglected disease of worldwide public health concern. Leptospira species can infect a wide range of wild and domestic mammals and lead to a spectrum of disease, including severe and fatal forms. Herein, we report for the first time a fatal Leptospira interrogans infection in a free-ranging nonhuman primate (NHP), a black-tufted marmoset. Read More

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Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein in Grown in EMJH Medium.

Pathogens 2021 Jul 6;10(7). Epub 2021 Jul 6.

Regional Medical Research Centre Port Blair, Indian Council of Medical Research, Port Blair 744103, Andaman and Nicobar Islands, India.

Leptospirosis is a re-emerging form of zoonosis that is caused by the spirochete pathogen . Extracellular proteins play critical roles in the pathogenicity and survival of this pathogen in the host and environment. Extraction and analysis of extracellular proteins is a difficult task due to the abundance of enrichments like serum and bovine serum albumin in the culture medium, as is distinguishing them from the cellular proteins that may reach the analyte during extraction. Read More

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Presence of spp. and absence of spp. in urban rodents of Buenos Aires province, Argentina.

Pathog Glob Health 2021 Aug 2:1-8. Epub 2021 Aug 2.

KB One Health LLC, Fort Collins.

Big cities of Argentina are characterized by a strong social and economic fragmentation. This context enables the presence of urban rodents in close contact to the human population, mostly in the peripheral areas of the cities. Urban rodents can harbor a large variety of zoonotic pathogens. Read More

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Detection and distribution of zoonotic pathogens in wild Norway rats () from Tehran, Iran.

New Microbes New Infect 2021 Jul 24;42:100908. Epub 2021 Jun 24.

Pediatric Infection Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

This is the first study on the prevalence of vector-borne zoonotic pathogens found in () in urban areas of Tehran, Iran. Serological tests were used to detect IgG antibodies against () and spp. using a commercial qualitative rat ELISA kit. Read More

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APRANK: Computational Prioritization of Antigenic Proteins and Peptides From Complete Pathogen Proteomes.

Front Immunol 2021 15;12:702552. Epub 2021 Jul 15.

Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde" (IIB), Universidad de San Martín (UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

Availability of highly parallelized immunoassays has renewed interest in the discovery of serology biomarkers for infectious diseases. Protein and peptide microarrays now provide a rapid, high-throughput platform for immunological testing and validation of potential antigens and B-cell epitopes. However, there is still a need for tools to prioritize and select relevant probes when designing these arrays. Read More

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Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of serovar Pomona in canine leptospirosis.

Iran J Vet Res 2021 ;22(2):120-128

Ph.D. Student in Biotechnology, Institute of Chemistry, Academia Sinica University, Nankang, Taipei, Taiwan.

Background: Canine leptospirosis is a serious public health concern.

Aims: This study aims to investigate the feasibility of conserved first to fifth domains of recombinant immunoglobulin like protein B antigen (rLigBCon1-5) as a serodiagnostic marker for detecting canine leptospirosis.

Methods: A total of 340 unvaccinated canine serum samples were screened using microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent assay (I-ELISA). Read More

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January 2021

Genomic data of HP358 isolated from rodent captured from the human leptospirosis suspected areas of Selangor state, Malaysia.

Data Brief 2021 Aug 10;37:107261. Epub 2021 Jul 10.

Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

The data provided in this article is the genomic sequence of new isolate, strain HP358 ( HP358) isolated from rodent, , captured from the human leptospirosis suspected area, in forest environment, Hulu Perdik, Selangor. The kidney of the rodent was cultured, and the genomic DNA of pure isolate was extracted and sequenced. The assembly of genome generated 118 contigs with N50 of 133,176bp. Read More

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Acute kidney injury with partial Fanconi syndrome in a patient with leptospirosis: a case report.

J Med Case Rep 2021 Jul 23;15(1):358. Epub 2021 Jul 23.

Department of General Internal Medicine, Geneva University Hospital, Rue Gabrielle-Perret-Gentil 4, 1205, Geneva, Switzerland.

Background: Leptospirosis is an underdiagnosed bacterial infection with nonspecific symptoms, hence, a diagnostic challenge. Identifying a case of leptospirosis in Switzerland is uncommon. Although kidney complications are frequent in severe forms, including tubular dysfunction, observing this complication is rare in our country. Read More

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A Novel Protein LIC13086 Inhibits Fibrin Clot Formation and Interacts With Host Components.

Front Cell Infect Microbiol 2021 1;11:708739. Epub 2021 Jul 1.

Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil.

Leptospirosis is a neglected zoonosis, caused by pathogenic spirochetes bacteria of the genus . The molecular mechanisms of leptospirosis infection are complex, and it is becoming clear that leptospires express several functionally redundant proteins to invade, disseminate, and escape the host's immune response. Here, we describe a novel leptospiral protein encoded by the gene LIC13086 as an outer membrane protein. Read More

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Shedding and Genetic Diversity of Leptospira spp. From Urban Stray Dogs in Klang Valley, Malaysia.

Top Companion Anim Med 2021 Jul 13;45:100562. Epub 2021 Jul 13.

Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Leptospirosis is an endemic zoonoses of global proportions. Stray dogs have been postulated to play a role in disease transmission; however, supporting information are still limited. Roaming behavior may not only predispose the dogs to infection, but could also contribute to disease spread. Read More

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Molecular detection and characterization of pathogenic Leptospira species in bats (Chiroptera) roosting in human habitats in Nigeria, West Africa.

Zoonoses Public Health 2021 Jul 12. Epub 2021 Jul 12.

Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.

Leptospirosis is a neglected zoonosis with a nearly global distribution. In order to determine the role of bats in the epidemiology of leptospirosis in Nigeria, a total of 231 bats belonging to three families, Pteropodidae (n = 117), Molossidae (n = 107) and Nycteridae (n = 17), roosting in human habitats were screened by PCR and sequencing for the detection of pathogenic Leptospira species. DNA extracted from the kidneys of bats were subjected to conventional PCR targeting the rrs1, rrs2, flaB and secY genes for the detection of pathogenic Leptospira spp. Read More

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