2,049 results match your criteria leishmania tropica

Bioactive levan type exopolysaccharide produced by ZMR7: characterization and optimization for enhanced production.

J Microbiol Biotechnol 2021 Apr 5. Epub 2021 Apr 5.

Department of Botany and Microbiology, Faculty of Science, Cairo University, 12613, Giza, Egypt.

Levan is an industrially important functional biopolymer with considerable applications in food and pharmaceutical fields owing to its safety and biocompatibility. Herein, levan type exopolysaccharide (EPS) produced by ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, H, and C NMR spectroscopy. The maximum production of levan (28. Read More

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Novel coumarin-isatin hybrids as potent antileishmanial agents: Synthesis, in silico and in vitro evaluations.

Bioorg Chem 2021 Mar 10;110:104816. Epub 2021 Mar 10.

Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan. Electronic address:

Leishmaniasis being one of the six major tropical diseases that affects nearly 0.7-1.3 million people annually, has so far limited and high toxic therapeutic options. Read More

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Identification of species by high-resolution melting analysis in newly emerged foci in Sabzevar, northeast of Iran.

J Parasit Dis 2021 Mar 24;45(1):191-196. Epub 2020 Oct 24.

Leishmaniasis Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.

Cutaneous leishmaniasis (CL) is a zoonotic disease with 1 to 1.5 million annual incidences. Microscopic examination of the Giemsa stained slides is the most common diagnostic method for CL. Read More

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Correction to: The leishmanicidal efect of Lucilia sericata larval saliva and hemolymph on in vitro Leishmania tropica.

Parasit Vectors 2021 Mar 15;14(1):155. Epub 2021 Mar 15.

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

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Epidemiological and genetic characteristics of asymptomatic canine leishmaniasis and implications for human Leishmania infections in Egypt.

Zoonoses Public Health 2021 Mar 14. Epub 2021 Mar 14.

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Leishmaniasis is a neglected zoonotic disease that poses significant veterinary and public health risks in developing countries. Dogs act as a reservoir host for leishmaniasis transmitted to humans. A total of 108 human cases of cutaneous leishmaniasis (CL) were identified in the Al-Houd Al-Marsoud Hospital in Cairo, Egypt, during 2018. Read More

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Genetic diversity of Leishmania tropica: Unexpectedly complex distribution pattern.

Acta Trop 2021 Mar 11;218:105888. Epub 2021 Mar 11.

Life Science Research Centre, Faculty of Science, University of Ostrava, 702 00 Ostrava, Czech Republic; Zoological Institute of the Russian Academy of Sciences, 199034 St. Petersburg, Russia. Electronic address:

In this study, we characterized a collection of clinical samples obtained from Syrian and Turkish patients with cutaneous leishmaniasis using internal transcribed spacer 1 (ITS1) sequences. All obtained sequences belonged to Leishmania tropica. Combining them with those available from GenBank allowed us performing a broad-scale analysis of genetic diversity for this species. Read More

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Entomological study in an anthroponotic cutaneous leishmaniasis focus in Morocco: Fauna survey, Leishmania infection screening, molecular characterization and MALDI-TOF MS protein profiling of relevant Phlebotomus species.

Transbound Emerg Dis 2021 Mar 8. Epub 2021 Mar 8.

Laboratory of Parasitology and Vector-Borne-Diseases, Institut Pasteur du Maroc, Casablanca, Morocco.

In Morocco, leishmaniases are a major public health problem due to their genetic diversity and geographical distribution. Cutaneous leishmaniasis (CL) is an infectious disease caused by various species of Leishmania and transmitted typically by bite of phlebotomine sand flies. This study identifies sand fly fauna in Ibaraghen village, province of Azilal, which is a focus of CL, by combination of morphological and molecular methods (sequencing of COI gene, MALDI-TOF MS protein profiling). Read More

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Complicated cutaneous leishmaniasis caused by an imported case of Leishmania tropica in Japan: a case report.

Trop Med Health 2021 Mar 6;49(1):20. Epub 2021 Mar 6.

Department of Infectious Diseases, Hiroshima University Hospital, 1-2-3 Minamiku Kasumi, Minamiku, Hiroshima City, Hiroshima, 734-8551, Japan.

Background: Leishmaniasis is not endemic in Japan, and imported cases are rare. However, there are increasing concerns regarding imported cases of cutaneous leishmaniasis from endemic countries to Japan. This report describes a case of imported cutaneous leishmaniasis that was diagnosed and treated in Japan. Read More

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Chemical and Bioinformatics Analyses of the Anti-Leishmanial and Anti-Oxidant Activities of Hemp Essential Oil.

Biomolecules 2021 Feb 12;11(2). Epub 2021 Feb 12.

Department of Pharmacy, University of Lahore, Islamabad Campus, Islamabad 54590, Pakistan.

Industrial hemp is a multiuse crop that has been widely cultivated to produce fibers and nutrients. The capability of the essential oil (EO) from inflorescences as antimicrobial agent has been reported. However, literature data are still lacking about the hemp EO antiprotozoal efficacy in vivo. Read More

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February 2021

Updates on Geographical Dispersion of Parasites Causing Cutaneous Affections in Algeria.

Pathogens 2021 Feb 25;10(3). Epub 2021 Feb 25.

Parasitology-Mycology Department, Avicenne Hospital, AP-HP, 93009 Bobigny, France.

Leishmaniases are neglected tropical diseases of public health concern in Algeria. To update the geographical distribution of spp. causing cutaneous affection, we examined a set of Giemsa-stained smears prepared from skin lesions of the patients suspected to have cutaneous leishmaniasis (CL) in various geographical areas in Algeria. Read More

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February 2021

Stage-Specific Differential Gene Expression of Glutathione Peroxidase in and .

Rep Biochem Mol Biol 2020 Oct;9(3):324-330

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: and are the etiological agents of cutaneous leishmaniosis. Leishmania species cause a board spectrum of phenotypes. A small number of genes are differentially expressed between them that have likely an important role in the disease phenotype. Read More

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October 2020

A long-lasting emerging epidemic of anthroponotic cutaneous leishmaniasis in southeastern Iran: population movement and peri-urban settlements as a major risk factor.

Parasit Vectors 2021 Feb 24;14(1):122. Epub 2021 Feb 24.

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Background: Epidemics of cutaneous leishmaniasis (CL) are occurring more frequently and spreading faster and farther than before in many areas of the world. The present study aimed to assess a long-lasting emerging epidemic (2005-2019) of 5532 cases with anthroponotic CL (ACL) in peri-urban areas of Kerman city in southeastern Iran.

Methods: This descriptive-analytical study was carried out for 15 years in Kerman province, southeastern Iran. Read More

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February 2021

Identification of differential protein expression and putative drug target in metacyclic stage of Leishmania major and Leishmania tropica: A quantitative proteomics and computational view.

Comp Immunol Microbiol Infect Dis 2021 Apr 27;75:101617. Epub 2021 Jan 27.

Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Cutaneous leishmaniasis (CL) is an infectious disease that commonly caused by Leishmania (L.) major and L.tropica. Read More

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The potential role and apoptotic profile of three medicinal plant extracts on by MTT assay, macrophage model and flow cytometry analysis.

Parasite Epidemiol Control 2021 Feb 10;12:e00201. Epub 2021 Jan 10.

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Introduction: Treatment of leishmaniasis with conventional synthetic drugs is a major global challenge. This study was designed to explore the leishmanicidal activity and apoptotic profile of three leaf extracts on stages.

Methods: The plants of and were gathered from Anbarabbad county, in the southeastern part of Kerman province and extracted by maceration method using methanol alcohol. Read More

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February 2021

Effectiveness of miltefosine in cutaneous leishmaniasis caused by Leishmania tropica in Pakistan after antimonial treatment failure or contraindications to first line therapy-A retrospective analysis.

PLoS Negl Trop Dis 2021 Jan 28;15(1):e0008988. Epub 2021 Jan 28.

Médecins Sans Frontières, Amsterdam, the Netherlands.

Background: Cutaneous leishmaniasis (CL) is a neglected tropical skin disease, caused by Leishmania protozoa. In Pakistan, where CL caused by L. tropica is highly endemic, therapy with pentavalent antimonials is the standard of care, but has significant toxicity when used in systemic therapy, while are no evidence-based safer alternative treatment options for L. Read More

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January 2021

A Nuclear Magnetic Resonance-Based Metabolomic Study to Identify Metabolite Differences between Iranian Isolates of major and .

Iran J Med Sci 2021 Jan;46(1):43-51

Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Cutaneous leishmaniasis caused by species () is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between , we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of and by proton nuclear magnetic resonance (H-NMR).

Methods: and were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. Read More

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January 2021

Design, Synthesis and In Vitro Investigation of Novel Basic Celastrol Carboxamides as Bio-Inspired Leishmanicidal Agents Endowed with Inhibitory Activity against Hsp90.

Biomolecules 2021 Jan 5;11(1). Epub 2021 Jan 5.

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.

The natural triterpene celastrol () is here used as lead compound for the design and synthesis of a panel of eleven carboxamides that were tested in vitro for their growth inhibitory activity against and parasites. Among them, in vitro screening identified four basic carboxamides endowed with nanomolar leishmanicidal activity, against both the promastigotes and the intramacrophage amastigotes forms. These compounds also showed low toxicity toward two human (HMEC-1 and THP-1) and one murine (BMDM) cell lines. Read More

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January 2021

The leishmanicidal effect of Lucilia sericata larval saliva and hemolymph on in vitro Leishmania tropica.

Parasit Vectors 2021 Jan 11;14(1):40. Epub 2021 Jan 11.

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: Leishmaniasis is a major parasitic disease worldwide, except in Australia and Antarctica, and it poses a significant public health problem. Due to the absence of safe and effective vaccines and drugs, researchers have begun an extensive search for new drugs. The aim of the current study was to investigate the in vitro leishmanicidal activity of larval saliva and hemolymph of Lucilia sericata on Leishmania tropica. Read More

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January 2021

Investigation of Phlebotominae (Diptera: Psychodidae) Fauna, Seasonal Dynamics, and Natural Leishmania spp. Infection in Muğla, Southwest of Turkey.

Acta Trop 2021 Apr 9;216:105827. Epub 2021 Jan 9.

Department of Parasitology, Faculty of Medicine, Ege University, Izmir, Turkey.

Due to its geographical location, Muğla province is one of the most frequently used places by refugees. Although leishmaniasis have been previously reported in this region, there is a lack of information on the etiological agent and possible vectors. The main objectives of this study were; i) to investigate the sand fly fauna, ii) to reveal the natural Leishmania spp. Read More

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First molecular identification of Leishmania major in Phlebotomus papatasi in an outbreak cutaneous leishmaniasis area in Iraq.

Acta Trop 2021 Mar 29;215:105807. Epub 2020 Dec 29.

Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan. Electronic address:

Cutaneous leishmaniasis (CL) is transmitted by Phlebotomine sand fly vectors, among which Phlebotomus papatasi is prevalent in Western Asia, Northern Africa and Southern Europe, and it is known as a vector for Leishmania major parasite in the world. However, in Iraq, morphological studies showed that P. papatasi is a predominant sand fly species and hypothesised to transmit CL causing Leishmania species including L. Read More

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Epidemiological and pathological characteristics of Cutaneous Leishmaniasis from Baluchistan Province of Pakistan.

Parasitology 2021 Apr 23;148(5):591-597. Epub 2020 Dec 23.

Department of Biosciences, COMSATS University Islamabad, Park Road, Chak Shahzad, Islamabad, Pakistan.

Cutaneous Leishmaniasis (CL) is considered a neglected tropical disease which in Pakistan can now be considered as a growing public health problem. The exact figures on the magnitude of the disease are lacking both at the national and regional level and only a few health centres are available for diagnosis of CL. The present study was designed to identify the epidemiology of CL infection from August 2018 to December 2019 and to assess clinical aspects of CL in Baluchistan Province of Pakistan. Read More

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Evolution of antigen-specific immune responses in cutaneous leishmaniasis patients.

Parasite Immunol 2021 Apr 15;43(4):e12814. Epub 2021 Feb 15.

Center for Research & Training in Skin Diseases & Leprosy (CRTSDL), Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Aims: Despite immunization appearing to be the most appropriate strategy for long-term control of the vector-borne leishmaniases, no sustainable vaccine is currently available against any form of leishmaniasis. We therefore evaluated, in the context of vaccine antigen candidates, antigen-specific immune response at various stages of cutaneous leishmaniasis (CL).

Methods And Results: Peripheral blood mononuclear cells (PBMC) isolated from healthy volunteers and CL patients (caused by either Leishmania major or L tropica) were incubated with crude Leishmania proteins (soluble Leishmania antigen; SLA), single recombinant proteins (TSA, LeIF, LmSTI1) or chimeric fusion proteins (LEISH-F2 and LEISH-F3). Read More

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Evaluation of a kDNA-Based qPCR Assay for the Detection and Quantification of Old World Species.

Microorganisms 2020 Dec 16;8(12). Epub 2020 Dec 16.

Department of Biomolecular Sciences, University of Urbino "Carlo Bo", 61029 Urbino, PU, Italy.

The parasite protozoan , the causative agent of leishmaniasis, includes two subgenera of medical interest: () and (). Parasite species detection and characterization is crucial to choose treatment protocols and to monitor the disease evolution. Molecular approaches can speed up and simplify the diagnostic process. Read More

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December 2020

Phytofabrication of cobalt oxide nanoparticles from Rhamnus virgata leaves extract and investigation of different bioactivities.

Microsc Res Tech 2021 Feb 17;84(2):192-201. Epub 2020 Dec 17.

Department of Plant Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.

The tunable cobalt oxide nanoparticles (CoONPs) are produced due to the phytochemicals present in Rhamnus virgata (RhV) leaf extract which functions as reducing and stabilization agents. The synthesis of CoONPs was confirmed using different analytical techniques: UV-Vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamics light scatterings (DLS), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray, and Raman spectroscopy analyses. Furthermore, multiple biological activities were performed. Read More

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February 2021

The potential therapeutic role of PTR1 gene in non-healing anthroponotic cutaneous leishmaniasis due to Leishmania tropica.

J Clin Lab Anal 2021 Mar 7;35(3):e23670. Epub 2020 Dec 7.

Leishmaniasis Research Centre, Kerman University of Medical Sciences, Kerman, Iran.

Background: Drug resistance is a common phenomenon frequently observed in countries where leishmaniasis is endemic. Due to the production of the pteridine reductase enzyme (PTR1), drugs lose their efficacy, and consequently, the patient becomes unresponsive to treatment. This study aimed to compare the in vitro effect of meglumine antimoniate (MA) on non- healing Leishmania tropica isolates and on MA transfected non-healing one to PTR1. Read More

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Leishmaniasis and phlebotomine sand flies in Oman Sultanate.

Parasite 2020 27;27:68. Epub 2020 Nov 27.

Université de Reims Champagne-Ardenne, EA7510, Université de Reims Champagne-Ardenne, Faculté de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims cedex, France - ANSES, USC Transmission Vectorielle et Épidémiosurveillance de Maladies Parasitaires (VECPAR), 51100 Reims, France - Laboratoire de Parasitologie, Pôle de Biologie, Centre Hospitalier Universitaire de Reims, 51100 Reims, France.

There are few data on leishmaniases and sandflies in Oman Sultanate. We carried out an eco-epidemiological study in 1998 in the two main mountains of the country, the Sharqiyah and the Dhofar. This study allowed us to isolate and identify three Leishmania strains from patients exhibiting cutaneous leishmaniasis. Read More

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February 2021

Comparison of Three Different Therapies for Cutaneous Leishmaniasis and Identification of the Etiologic Isolates in Isfahan, Iran.

Arch Iran Med 2020 11 1;23(11):740-748. Epub 2020 Nov 1.

Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Background: In Iran, zoonotic and anthroponotic cutaneous leishmaniasis (CL) are caused by Leishmania major and L. tropica respectively. Despite extensive studies, no effective therapies have ever been reported for CL. Read More

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November 2020

In vitro anti-leishmanial activity of Prunus armeniaca fractions on Leishmania tropica and molecular docking studies.

J Photochem Photobiol B 2020 Dec 3;213:112077. Epub 2020 Nov 3.

Department of Zoology, Faculty of Biological Science, Quaid-i-Azam University Islamabad, 45320, Pakistan.

Prunus armeniaca (L.) is a member of the Rosaceae, subfamily Prunoideae, shows anticancer, antitubercular, antimutagenic, antimicrobial, antioxidant, and cardioprotective activities. Here we fractionated the leaves extract of this highly medicinally important plant for antileishmanial activity. Read More

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December 2020

Cutaneous Leishmaniasis Treated with Miltefosine: A Case Series of 10 Paediatric Patients.

Acta Derm Venereol 2020 Nov;100(18):adv00322

Department of Dermatology, Rabin Medical Center, Beilinson Hospital Petach Tikva, Petach Tikva 49100, Israel. E-mail:

Cutaneous leishmaniasis poses a therapeutic challenge in the paediatric population. The aim of this study was to assess the efficacy and safety of miltefosine treatment for Old World cutaneous leishmaniasis in paediatric patients. A multicentre retrospective review of 10 children (≤ 18 years of age) with cutaneous leishmaniasis treated with miltefosine in Israel was performed. Read More

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November 2020